true_scfv_var_s1_sequences.json

{
  "candidate_id": "var_s1",
  "parent_id": "WT_SCFV",
  "representation": "true_scfv",
  "chain_B_scfv": "EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGNTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIQMTQSPSSLSASVGDRVTITCRASQDVHTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIK",
  "chain_C_HER2_ECD": "TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLSPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTATQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDSNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSIWKFPDEEGACQPCPIN",
  "linker_sequence": "GGGGSGGGGSGGGGS",
  "mutations_vs_wt": ["B:57 Y->N", "A:30 N->H"],
  "mutations_vs_d70": [],
  "scfv_aware_remap_splitter_required": true,
  "linker_aware_contact_mask_required": true,
  "source": "ProteinMPNN true scFv A30/B57 variation smoke (job 6a50b64d6d2b10c09d677a7b), sample 1",
  "validation_status": "UNVALIDATED_PENDING_BOLTZ2",
  "downstream_validation_required": true,
  "allowed_archive_update": false,
  "note": "ProteinMPNN-designed true scFv candidate; VH-(G4S)3-VL; 242 aa; linker intact; A30/B57 allowed mutations only; UNVALIDATED until Boltz2 + HER2 remap + scFv split + linker mask + C:591 + interface-quality validation passes"
}