| { | |
| "schema_version": "1.0", | |
| "level": "species", | |
| "genus": "Comamonas", | |
| "species": "testosteroni", | |
| "overview": { | |
| "short": "Comamonas testosteroni is a Gram-negative, aerobic, non-fermentative rod primarily associated with environmental sources.", | |
| "clinical_context": "Rare opportunistic pathogen occasionally isolated from bloodstream, respiratory, or wound infections." | |
| }, | |
| "expected_fields": { | |
| "Gram Stain": "Negative", | |
| "Shape": "Rods", | |
| "Motility": "Positive", | |
| "Capsule": "Negative", | |
| "Spore Formation": "Negative", | |
| "Haemolysis": "Negative", | |
| "Haemolysis Type": "None", | |
| "Media Grown On": [ | |
| "Blood Agar", | |
| "Nutrient Agar" | |
| ], | |
| "Colony Morphology": [ | |
| "Smooth", | |
| "Grey", | |
| "Non-pigmented" | |
| ], | |
| "Oxygen Requirement": "Aerobic", | |
| "Growth Temperature": "20//42", | |
| "Catalase": "Positive", | |
| "Oxidase": "Positive", | |
| "Indole": "Negative", | |
| "Urease": "Negative", | |
| "Citrate": "Positive", | |
| "Methyl Red": "Negative", | |
| "VP": "Negative", | |
| "H2S": "Negative", | |
| "DNase": "Negative", | |
| "ONPG": "Negative", | |
| "Coagulase": "Negative", | |
| "Gelatin Hydrolysis": "Variable", | |
| "Esculin Hydrolysis": "Negative", | |
| "Nitrate Reduction": "Positive", | |
| "NaCl Tolerant (>=6%)": "Negative", | |
| "Lipase Test": "Negative", | |
| "Lysine Decarboxylase": "Negative", | |
| "Ornithine Decarboxylase": "Negative", | |
| "Ornitihine Decarboxylase": "Negative", | |
| "Arginine dihydrolase": "Negative", | |
| "Glucose Fermentation": "Negative", | |
| "Lactose Fermentation": "Negative", | |
| "Sucrose Fermentation": "Negative", | |
| "Maltose Fermentation": "Negative", | |
| "Mannitol Fermentation": "Negative", | |
| "Sorbitol Fermentation": "Negative", | |
| "Xylose Fermentation": "Negative", | |
| "Rhamnose Fermentation": "Negative", | |
| "Arabinose Fermentation": "Negative", | |
| "Raffinose Fermentation": "Negative", | |
| "Trehalose Fermentation": "Negative", | |
| "Inositol Fermentation": "Negative", | |
| "Gas Production": "Negative", | |
| "TSI Pattern": "Unknown", | |
| "Colony Pattern": "Unknown", | |
| "Pigment": "None", | |
| "Motility Type": "Polar" | |
| }, | |
| "species_markers": [ | |
| { | |
| "field": "Citrate", | |
| "value": "Positive", | |
| "importance": "high", | |
| "notes": "Major discriminator from Comamonas kerstersii." | |
| }, | |
| { | |
| "field": "Nitrate Reduction", | |
| "value": "Positive", | |
| "importance": "high", | |
| "notes": "Supports identification when combined with citrate positivity." | |
| }, | |
| { | |
| "field": "Environmental Source", | |
| "value": "Common", | |
| "importance": "medium", | |
| "notes": "Frequently isolated from soil and water." | |
| } | |
| ], | |
| "common_confusions": [ | |
| { | |
| "species": "Comamonas kerstersii", | |
| "reason": "Overlapping morphology and non-fermentative biochemistry." | |
| }, | |
| { | |
| "genus": "Achromobacter", | |
| "reason": "Shared oxidase-positive, non-fermentative profile." | |
| } | |
| ], | |
| "when_to_question_identification": [ | |
| "Citrate negativity suggests Comamonas kerstersii.", | |
| "Clinical isolation from intra-abdominal infections favors C. kerstersii.", | |
| "Glucose fermentation excludes Comamonas species." | |
| ], | |
| "recommended_next_tests": [ | |
| { | |
| "test": "Citrate utilization", | |
| "reason": "Primary biochemical discriminator.", | |
| "api_kit": "API 20NE" | |
| }, | |
| { | |
| "test": "Nitrate reduction", | |
| "reason": "Supports species-level separation.", | |
| "api_kit": "API 20NE" | |
| }, | |
| { | |
| "test": "MALDI-TOF MS", | |
| "reason": "Definitive species identification.", | |
| "api_kit": "Not applicable" | |
| } | |
| ] | |
| } |