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DNA- and RNA-processing pathways are integrated and interconnected in the eukaryotic nucleus to allow efficient gene expression and to maintain genomic stability . The recruitment of DNA Topoisomerase I ( Topo I ) , an enzyme controlling DNA supercoiling and acting as a specific kinase for the SR-protein family of spli...
DNA Topoisomerase I ( Topo I ) is a very well known enzyme capable of removing DNA topological constrains during transcription . In mammals , Topo I also harbours an intrinsic protein kinase activity required to achieve specific phosphorylation of factors in charge of maturating the transcript and exporting it from the...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "developmental", "biology", "cell", "biology/nuclear", "structure", "and", "function", "cell", "biology/developmental", "molecular", "mechanisms", "developmental", "biology/cell", "differentiation", "biochemistry/transcription", "and", "translation", "developmenta...
2010
The SR Protein B52/SRp55 Is Required for DNA Topoisomerase I Recruitment to Chromatin, mRNA Release and Transcription Shutdown
The role of the host immunity in determining leprosy clinical forms and complications is well recognized , implying that changes in the immune status may interfere with several aspects of the disease . Therefore , we hypothesized that the presence of viral co-infections and associated immunological changes will have a ...
The clinical and social impact of leprosy , a disease caused by Mycobacterium leprae , is due to the deformities and disabilities that are consequences of skin and peripheral nerve inflammation . We believe that leprosy patients who also have an associated viral infection will have a worse outcome . This can be due to ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Viral Co-infection and Leprosy Outcomes: A Cohort Study
Retroviruses encode cis-acting RNA nuclear export elements that override nuclear retention of intron-containing viral mRNAs including the full-length , unspliced genomic RNAs ( gRNAs ) packaged into assembling virions . The HIV-1 Rev-response element ( RRE ) recruits the cellular nuclear export receptor CRM1 ( also kno...
How cellular and viral mRNAs are trafficked inside the cell is poorly understood . In this study , we use fluorescence microscopy to visualize HIV-1 mRNA nuclear export , translation , trafficking , and virus particle assembly in single living cells , focusing on the full-length viral genomic RNAs ( gRNAs ) that are pa...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "centrosomes", "pathology", "and", "laboratory", "medicine", "microtubules", "pathogens", "messenger", "rna", "microbiology", "viral", "structure", "retroviruses", "viruses", "immunodeficiency", "viruses", "rna", "viruses", "cell",...
2016
HIV-1 and M-PMV RNA Nuclear Export Elements Program Viral Genomes for Distinct Cytoplasmic Trafficking Behaviors
Spermatogenesis is the process by which male gametes are formed from a self-renewing population of spermatogonial stem cells ( SSCs ) residing in the testis . SSCs represent less than 1% of the total testicular cell population in adults , but must achieve a stable balance between self-renewal and differentiation . Once...
Spermatogenesis is the process by which male gametes–mature spermatozoa–are produced in the testis . This process requires exquisite control over many developmental transitions , including the self-renewal of the germline stem cell population , commitment to meiosis , and ultimately , spermiogenesis . While much is kno...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "meiosis", "medicine", "and", "health", "sciences", "spermatocytes", "cell", "cycle", "and", "cell", "division", "cell", "processes", "marker", "genes", "spermatogonia", "reproductive", "physiology", "germ", "cells", "protein", "expression", "molecular", "biology", "t...
2019
Dynamic transcriptome profiles within spermatogonial and spermatocyte populations during postnatal testis maturation revealed by single-cell sequencing
While primarily a mosquito-borne virus , Zika virus ( ZIKV; genus Flavivirus in the Flaviviridae family ) is capable of being sexually transmitted . Thirty to fifty percent of men with confirmed ZIKV infection shed ZIKV RNA in their semen , and prolonged viral RNA shedding in semen can occur for more than 6 months . Th...
While Zika virus ( ZIKV ) is primarily a mosquito-borne virus , there are now confirmed sexual transmission cases of ZIKV from infected males to their partners . Using a previously established mouse model of sexual transmission , ZIKV was herein demonstrated to infect the testes and epididymides concurrently , suggesti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "reproductive", "system", "immune", "cells", "body", "fluids", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "microbiology", "germ", "cells", "viruses", "rna", "viruses", "sexually",...
2018
Infection of epididymal epithelial cells and leukocytes drives seminal shedding of Zika virus in a mouse model
Rift Valley fever ( RVF ) is endemic in most parts of Africa and has also been reported to occur in the Arabian Peninsula . It is responsible for significant morbidity and mortality , particularly in livestock , but also in humans . During the last two decades several outbreaks of RVF have been reported in countries in...
Rift Valley fever ( RVF ) is a mosquito-borne disease that results in severe negative impact on human and animal health and the economy . Outbreaks of RVF occur sporadically when heavy rains favour the breeding and emergence of mosquito vectors of the virus . Rift Valley fever has been reported in many African countrie...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "animal", "types", "veterinary", "diseases", "veterinary", "epidemiology", "small", "animals", "veterinary", "virology", "zoonotic", "diseases", "veterinary", "science" ]
2013
Serological Evidence of Rift Valley Fever Virus Circulation in Sheep and Goats in Zambézia Province, Mozambique
Next-generation sequencing of DNA provides an unprecedented opportunity to discover rare genetic variants associated with complex diseases and traits . However , the common practice of first calling underlying genotypes and then treating the called values as known is prone to false positive findings , especially when g...
In next-generation sequencing studies , there are typically systematic differences in sequencing qualities ( e . g . , depth ) between cases and controls , because the entire studies are rarely sequenced in exactly the same way . It has long been appreciated that , in the presence of such differences , the standard gen...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "sequencing", "techniques", "body", "weight", "medicine", "and", "health", "sciences", "applied", "mathematics", "variant", "genotypes", "genetic", "mapping", "simulation", "and", "modeling", "algorithms", "next-generation", "sequencing", "mathematics", "physiological", "...
2016
Testing Rare-Variant Association without Calling Genotypes Allows for Systematic Differences in Sequencing between Cases and Controls
The formation and stability of synapses are key questions in neuroscience . Post-synaptic domains have been classically conceived as resulting from local insertion and turnover of proteins at the synapse . However , insertion is likely to occur outside the post-synaptic domains and advances in single-molecule imaging h...
Synapses mediate information transmission between neurons and are the physical support of memory . It has been realized that synapses are dynamic biological structures . Neurotransmitter receptors diffuse in the neuron membrane and synaptic scaffolding proteins are constantly renewed . We propose a biophysical model th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "nervous", "system", "electrophysiology", "neuroscience", "membrane", "proteins", "cellular", "structures", "and", "organelles", "animal", "cells", "proteins", "biophysics", "cell", "membranes", "cytoplasm", "physics", "biochemistr...
2017
An aggregation-removal model for the formation and size determination of post-synaptic scaffold domains
Leptospirosis , caused by Leptospira , is one of the most important of neglected emerging zoonotic diseases that has important impacts on public health worldwide . Polyclonal antibody ( pcAb ) therapy is a potential method to process a series of pathogens for which there are limited determination of treatment , such as...
Leptospirosis , caused by Leptospira , is one of the most important neglected emerging zoonotic diseases that has important impacts on public health worldwide . An urgent need exists for novel therapeutic agents directed against Leptospira without severe side-effects . This study demonstrates the efficacy of a rabbit p...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "antimicrobials", "medicine", "and", "health", "sciences", "liver", "leptospira", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "enzyme-linked", "immunoassays", "pathogens", "drugs", "immunology", "tropical", "diseases", "microbiology", "vertebrates"...
2016
Efficacy of the Rabbit Polyclonal Anti-leptospira Antibody against Homotype or Heterotype Leptospira Infection in Hamster
Simian varicella virus ( SVV ) , the etiologic agent of naturally occurring varicella in primates , is genetically and antigenically closely related to human varicella zoster virus ( VZV ) . Early attempts to develop a model of VZV pathogenesis and latency in nonhuman primates ( NHP ) resulted in persistent infection ....
Varicella zoster virus ( VZV ) is a ubiquitous herpesvirus that causes chickenpox ( varicella ) and establishes a life-long latent infection in humans . VZV can then reactivate causing herpes zoster , commonly known as shingles , a painful and debilitating disease that can be life-threatening in elderly and immunocompr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/infectious", "diseases", "of", "the", "nervous", "system", "immunology/immunity", "to", "infections", "immunology/immune", "response", "infectious", "diseases/viral", "infections" ]
2009
Simian Varicella Virus Infection of Rhesus Macaques Recapitulates Essential Features of Varicella Zoster Virus Infection in Humans
The synaptonemal complex ( SC ) is an intricate structure that forms between homologous chromosomes early during the meiotic prophase , where it mediates homolog pairing interactions and promotes the formation of genetic exchanges . In Drosophila melanogaster , C ( 3 ) G protein forms the transverse filaments ( TFs ) o...
Meiosis is a specialized type of cell division that is needed to produce sperm and egg cells , which carry only half the number of chromosomes of other cells in the body . Meiosis is required for reproduction , but abnormalities in chromosome number caused by errors in the process of meiosis are responsible for many bi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/recombination", "cell", "biology/cell", "growth", "and", "division", "molecular", "biology/chromosome", "structure", "genetics", "and", "genomics/chromosome", "biology" ]
2008
corona Is Required for Higher-Order Assembly of Transverse Filaments into Full-Length Synaptonemal Complex in Drosophila Oocytes
Recent steep declines in honey bee health have severely impacted the beekeeping industry , presenting new risks for agricultural commodities that depend on insect pollination . Honey bee declines could reflect increased pressures from parasites and pathogens . The incidence of the microsporidian pathogen Nosema ceranae...
Honey bee colonies are in decline in many parts of the world , in part due to pressures from a diverse assemblage of parasites and pathogens . The range and prevalence of the microsporidian pathogen Nosema ceranae has increased significantly in the past decade . Here we describe the N . ceranae genome , presenting geno...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "genetics", "and", "genomics/microbial", "evolution", "and", "genomics", "evolutionary", "biology/microbial", "evolution", "and", "genomics", "infectious", "diseases", "evolutionary", "biology/evolutionary", "and", "comparative", "genetics", "evolutionary", "biology/genomics", ...
2009
Genomic Analyses of the Microsporidian Nosema ceranae, an Emergent Pathogen of Honey Bees
Understanding cooperation in animal social groups remains a significant challenge for evolutionary theory . Observed behaviours that benefit others but incur some cost appear incompatible with classical notions of natural selection; however , these behaviours may be explained by concepts such as inclusive fitness , rec...
One of the key challenges facing evolutionary theory is understanding how cooperation and communication evolve in social systems . In many situations cooperation leads to higher net benefits to all , but a population of cooperators is vulnerable to invasion from exploitative strategies . When foraging , aiding others t...
[ "Abstract", "Introduction", "Model", "Results", "Discussion" ]
[ "evolutionary", "ecology", "mathematical", "computing", "mathematics", "ecology", "evolutionary", "biology", "biology", "evolutionary", "theory", "behavioral", "ecology" ]
2011
Signalling and the Evolution of Cooperative Foraging in Dynamic Environments
The bristle sensillum of the imago of Drosophila is made of four cells that arise from a sensory organ precursor cell ( SOP ) . This SOP is selected within proneural clusters ( PNC ) through a mechanism that involves Notch signalling . PNCs are defined through the expression domains of the proneural genes , whose activ...
The sensory organ precursor cell ( SOP ) that forms the mechanosensory bristles of the adult PNS of Drosophila is a paradigm to study neural precursor determination . The current model states that the SOP is selected in proneural clusters ( PNCs ) defined through the expression of the proneural genes . The selection oc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "and", "life", "sciences" ]
2015
A Re-examination of the Selection of the Sensory Organ Precursor of the Bristle Sensilla of Drosophila melanogaster
Despite antiretroviral therapy ( ART ) , some HIV-infected persons maintain lower than normal CD4+ T-cell counts in peripheral blood and in the gut mucosa . This incomplete immune restoration is associated with higher levels of immune activation manifested by high systemic levels of biomarkers , including sCD14 and D-d...
HIV infected people who receive antiretroviral therapy ( ART ) remain at higher risk of non-infectious complications such as cardiovascular disease . This risk is linked to persistent inflammation and immune activation and is higher in those with lower circulating CD4+ T-cell counts . IL-7 therapy can increase CD4+ and...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "infectious", "diseases", "clinical", "immunology", "immunology", "biology" ]
2014
Decreases in Colonic and Systemic Inflammation in Chronic HIV Infection after IL-7 Administration
Quorum sensing ( QS ) signaling allows bacteria to control gene expression once a critical population density is achieved . The Gram-negative human pathogen Pseudomonas aeruginosa uses N-acylhomoserine lactones ( AHL ) as QS signals , which coordinate the production of virulence factors and biofilms . These bacterial s...
The human pathogen Pseudomonas aeruginosa and other bacteria communicate with each other using quorum sensing ( QS ) . This is important for their growth , virulence , motility and the formation of biofilms . Furthermore , eukaryotic cells “listen and respond” to QS signaling , but the exact mechanisms and receptors on...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "mechanisms", "of", "signal", "transduction", "small", "molecules", "microbiology", "host-pathogen", "interaction", "bacterial", "diseases", "bacterial", "pathogens", "infectious", "diseases", "lipids", "medical", "microbiology", "microbial", "pathogens", "biolo...
2012
The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration
In many cell types , release of calcium ions is controlled by inositol 1 , 4 , 5-trisphosphate ( ) receptor channels . Elevations in concentration after intracellular release through receptors ( R ) can either propagate in the form of waves spreading through the entire cell or produce spatially localized puffs . The ap...
Calcium signals are important for a host of cellular processes such as neurotransmitter release , cell contraction and gene expression . While the principles of activation and spreading of calcium signals have been largely understood , it is much less clear how their spatio-temporal appearance is shaped . This issue is...
[ "Abstract", "Introduction", "Model", "Results", "Discussion" ]
[ "mechanisms", "of", "signal", "transduction", "signaling", "networks", "biophysics", "simulations", "calcium", "signaling", "signaling", "in", "cellular", "processes", "biochemistry", "simulations", "biophysics", "theory", "biology", "biophysics", "molecular", "biology", ...
2012
Termination of Ca2+ Release for Clustered IP3R Channels
The ability to examine the behavior of biological systems in silico has the potential to greatly accelerate the pace of discovery in diseases , such as stroke , where in vivo analysis is time intensive and costly . In this paper we describe an approach for in silico examination of responses of the blood transcriptome t...
Computational modeling aims to use mathematical and algorithmic principles to link components of biological systems to predict system behavior . In the past such models have described a small set of carefully studied molecular interactions ( proteins in signal transduction pathways ) or larger abstract components ( cel...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "neurobiology", "of", "disease", "and", "regeneration", "immunology", "computerized", "simulations", "neuroscience", "animal", "models", "model", "organisms", "mathematics", "regulatory", "networks", "biology", "differential", "equations", "mouse", "immune", "response", "...
2012
Modeling Dynamic Regulatory Processes in Stroke
The prevalence of rheumatic heart disease ( RHD ) in the Aboriginal population of the Australian Northern Territory is high , and Streptococcus pyogenes skin infections likely contribute to this . A promising candidate S . pyogenes “30mer” vaccine is composed of 30 pharyngitis associated type-specific antigens from the...
The bacterium Streptococcus pyogenes causes throat and skin infections . A danger from such infections is an immune response that attacks human heart tissue , leading to rheumatic heart disease , which is difficult to treat and potentially deadly . Disadvantaged populations such as the Indigenous people in remote tropi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "skin", "dermatology", "medicine", "and", "health", "sciences", "body", "fluids", "integumentary", "system", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "microbiology", "vaccines", "throat", "bacterial", "diseases", "streptococcus", "pyogenes"...
2019
Concerns for efficacy of a 30-valent M-protein-based Streptococcus pyogenes vaccine in regions with high rates of rheumatic heart disease
The Pacific region is an area unique in the world , composed of thousands of islands with differing climates and environments . The spreading and establishment of the mosquito Aedes aegypti in these islands might be linked to human migration . Ae . aegypti is the major vector of arboviruses ( dengue , chikungunya and Z...
Aedes aegypti is the major arbovirus vector in the Pacific region . The spread of this mosquito in the different islands seems to be linked to human activities at the beginning of the twentieth century . Since 2010 , occurrence of arbovirus outbreaks increased in this region , with the co-circulation of dengue , chikun...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2016
Genetic Diversity and Phylogeny of Aedes aegypti, the Main Arbovirus Vector in the Pacific
As the nervous system develops , there is an inherent variability in the connections formed between differentiating neurons . Despite this variability , neural circuits form that are functional and remarkably robust . One way in which neurons deal with variability in their inputs is through compensatory , homeostatic c...
As the nervous system develops , an intricate web of connections forms between nerve cells , leading to the assembly of signalling networks that are capable of complex computations . However , the number and strength of connections formed between nerve cells varies . We ask how nerve cells deal with this variability so...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience", "developmental", "biology" ]
2008
Structural Homeostasis: Compensatory Adjustments of Dendritic Arbor Geometry in Response to Variations of Synaptic Input
Mycobacterium tuberculosis ( Mtb ) resides in a long-lived phagosomal compartment that resists maturation . The manner by which Mtb antigens are processed and presented on MHC Class I molecules is poorly understood . Using human dendritic cells and IFN-γ release by CD8+ T cell clones , we examined the processing and pr...
Major Histocompatibility Complex Class I ( MHC-I ) generally serves to present peptides derived from cytosolic proteins to CD8+ T lymphocytes , thereby alerting the immune system that the cell is infected . The machinery required for MHC-I antigen processing and presentation is localized to the cytosol and endoplasmic ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/bacterial", "infections", "cell", "biology", "immunology/immunity", "to", "infections", "immunology/antigen", "processing", "and", "recognition" ]
2009
The Mycobacterium tuberculosis Phagosome Is a HLA-I Processing Competent Organelle
Numerous microbial pathogens modulate or interfere with cell death pathways in cultured cells . However , the precise role of host cell death during in vivo infection remains poorly understood . Macrophages infected by pathogenic species of Yersinia typically undergo an apoptotic cell death . This is due to the activit...
The ability of bacterial pathogens to modulate death of infected host cells is an important virulence determinant . For pathogenic members of the genus Yersinia , the type III secreted effector protein YopJ/YopP is required for Yersinia-induced macrophage death . The YopJ protein is expressed by Y . pseudotuberculosis ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "microbiology/cellular", "microbiology", "and", "pathogenesis", "microbiology/innate", "immunity" ]
2008
Reduced Secretion of YopJ by Yersinia Limits In Vivo Cell Death but Enhances Bacterial Virulence
Defining the exact mechanisms by which the brain processes visual objects and scenes remains an unresolved challenge . Valuable clues to this process have emerged from the demonstration that clusters of neurons ( “modules” ) in inferior temporal cortex apparently respond selectively to specific categories of visual sti...
Many reports suggest that different categories of visual stimuli are processed in correspondingly specific “modules” in the visual cortex . For instance , images of faces are processed in one cortical module ( the “fusiform face area” ) , while images of scenes are processed in an adjacent module ( the “parahippocampal...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/cognitive", "neuroscience", "neuroscience/sensory", "systems", "neuroscience" ]
2011
The “Parahippocampal Place Area” Responds Preferentially to High Spatial Frequencies in Humans and Monkeys
Adaptation of the chemotaxis sensory pathway of the bacterium Escherichia coli is integral for detecting chemicals over a wide range of background concentrations , ultimately allowing cells to swim towards sources of attractant and away from repellents . Its biochemical mechanism based on methylation and demethylation ...
Bacterial chemotaxis is a paradigm for sensory systems , and thus has attracted immense interest from biologists and modelers alike . Using this pathway , cells can sense chemical molecules in their environment , and bias their movement towards nutrients and away from toxins . To avoid over- or understimulation of the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/cell", "signaling", "biophysics/biomacromolecule-ligand", "interactions", "computational", "biology/signaling", "networks", "microbiology/cellular", "microbiology", "and", "pathogenesis", "computational", "biology/systems", "biology" ]
2010
Chemotactic Response and Adaptation Dynamics in Escherichia coli
Rift Valley fever virus ( RVFV ) is an important mosquito-borne veterinary and human pathogen that can cause severe disease including acute-onset hepatitis , delayed-onset encephalitis , retinitis and blindness , or a hemorrhagic syndrome . Currently , no licensed vaccine or therapeutics exist to treat this potentially...
Rift Valley fever ( RVF ) is an important neglected tropical disease that has caused severe epidemics and epizootics throughout Africa and the Arabian Peninsula . Severe outbreaks have involved tens of thousands of both human and livestock cases for which no effective , commercially available human vaccines or antivira...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "animal", "models", "of", "infection", "antivirals", "emerging", "infectious", "diseases", "virology", "emerging", "viral", "diseases", "biology", "microbiology", "host-pathogen", "interaction", "pathogenesis" ]
2013
Aerosol Exposure to Rift Valley Fever Virus Causes Earlier and More Severe Neuropathology in the Murine Model, which Has Important Implications for Therapeutic Development
There is still much unknown regarding the computational role of inhibitory cells in the sensory cortex . While modeling studies could potentially shed light on the critical role played by inhibition in cortical computation , there is a gap between the simplicity of many models of sensory coding and the biological compl...
Cortical function is a result of coordinated interactions between excitatory and inhibitory neural populations . In previous theoretical models of sensory systems , inhibitory neurons are often ignored or modeled too simplistically to contribute to understanding their role in cortical computation . In biophysical reali...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Modeling Inhibitory Interneurons in Efficient Sensory Coding Models
Mass drug administration ( MDA ) of praziquantel has been the intervention of choice against schistosomiasis but with limited success in interrupting the transmission . The development of anti-Schistosoma vaccines is underway . Our objective is to quantify the population-level impact of anti-Schistosoma vaccines when a...
A number of experimental anti-schistosomiasis vaccines are under development . Our results have implications on the vaccines’ design and implementation . Design: For optimal impact at the population-level we found that the best vaccines are the ones that substantially reduce the acquisition of new worms and that kill e...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "death", "rates", "invertebrates", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "demography", "immunology", "tropical", "diseases", "parasitic", "diseases", "animals", "vaccines", "preventive", "medicine", "gastropods", "neglected...
2017
Quantitative assessment of the impact of partially protective anti-schistosomiasis vaccines
The interactions of legumes with symbiotic nitrogen-fixing bacteria cause the formation of specialized lateral root organs called root nodules . It has been postulated that this root nodule symbiosis system has recruited factors that act in early signaling pathways ( common SYM genes ) partly from the ancestral mycorrh...
Legumes produce nodules in roots as the endosymbiotic organs for nitrogen-fixing bacteria , collectively called rhizobia . The symbiotic relationship enables legumes to survive on soil with poor nitrogen sources . The rhizobial infection triggers cell division in the cortex to generate root nodule primordia . The root ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "science", "plant", "microbiology", "plant", "evolution", "plant", "growth", "and", "development", "plant", "biology", "plant", "genetics", "biology" ]
2013
NODULE INCEPTION Directly Targets NF-Y Subunit Genes to Regulate Essential Processes of Root Nodule Development in Lotus japonicus
Lipid rafts are membrane microdomains specialized in the regulation of numerous cellular processes related to membrane organization , as diverse as signal transduction , protein sorting , membrane trafficking or pathogen invasion . It has been proposed that this functional diversity would require a heterogeneous popula...
Cellular membranes organize proteins related to signal transduction , protein sorting and membrane trafficking into the so-called lipid rafts . It has been proposed that the functional diversity of lipid rafts would require a heterogeneous population of raft domains with varying compositions . However , a mechanism for...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Spatio-temporal Remodeling of Functional Membrane Microdomains Organizes the Signaling Networks of a Bacterium
During antagonistic coevolution between viruses and their hosts , viruses have a major advantage by evolving more rapidly . Nevertheless , viruses and their hosts coexist and have coevolved , although the processes remain largely unknown . We previously identified Tm-1 that confers resistance to Tomato mosaic virus ( T...
Viruses rapidly evolve and adapt to their host organisms , and the evolutionary processes can be reproduced in the laboratory ( experimental evolution ) . In contrast , cellular organisms ( that can be viral hosts ) evolve much more slowly than viruses , but the fact that they have antiviral systems suggests that virus...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "protein", "interactions", "plant", "biology", "microbiology", "host-pathogen", "interaction", "mechanisms", "of", "resistance", "and", "susceptibility", "plant", "science", "plant", "pathology", "viral", "nucleic", "acid", "proteins", "plant", "genetics", "biology", "v...
2012
Coevolution and Hierarchical Interactions of Tomato mosaic virus and the Resistance Gene Tm-1
Lavender Foal Syndrome ( LFS ) is a lethal inherited disease of horses with a suspected autosomal recessive mode of inheritance . LFS has been primarily diagnosed in a subgroup of the Arabian breed , the Egyptian Arabian horse . The condition is characterized by multiple neurological abnormalities and a dilute coat col...
Genetic disorders affect many domesticated species , including the horse . In this study we have focused on Lavender Foal Syndrome , a seizure disorder that leads to suffering and death in foals soon after birth . A recessively inherited disorder , its occurrence is often unpredictable and difficult for horse breeders ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/animal", "genetics", "genetics", "and", "genomics/disease", "models", "genetics", "and", "genomics/genetics", "of", "disease", "genetics", "and", "genomics/bioinformatics", "genetics", "and", "genomics/medical", "genetics" ]
2010
Whole-Genome SNP Association in the Horse: Identification of a Deletion in Myosin Va Responsible for Lavender Foal Syndrome
Neural morphology and membrane properties vary greatly between cell types in the nervous system . The computations and local circuit connectivity that neurons support are thought to be the key factors constraining the cells’ biophysical properties . Nevertheless , additional constraints can be expected to further shape...
Cellular design varies widely across neurons . Evolution is thought to have adapted the biophysical properties of each cell type to better support their specific function . The most influential among these neuronal parameters include ion channels and morphology . At the same time , we know that the brain is disproporti...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "acoustics", "medicine", "and", "health", "sciences", "action", "potentials", "ears", "membrane", "potential", "electrophysiology", "neuroscience", "nerve", "fibers", "neuronal", "dendrites", "excitatory", "postsynaptic", "potentials", "animal", "cells", "axons", "head", ...
2018
Function and energy consumption constrain neuronal biophysics in a canonical computation: Coincidence detection
It is unclear whether dengue serotypes differ in their propensity to cause severe disease . We analyzed differences in serotype-specific disease severity in children presenting for medical attention in Bangkok , Thailand . Prospective studies were conducted from 1994 to 2006 . Univariate and multivariate logistic and m...
The four dengue viruses ( DENV ) represent the most common human arbovirus infections in the world and are currently a challenging problem , particularly in the tropical and subtropical regions of Asia and the Americas . Infection with DENV may produce symptoms of varying severity . While access to care , appropriate i...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "mathematics/statistics", "infectious", "diseases", "public", "health", "and", "epidemiology", "virology" ]
2010
Serotype-Specific Differences in the Risk of Dengue Hemorrhagic Fever: An Analysis of Data Collected in Bangkok, Thailand from 1994 to 2006
The Th-inducing POK ( Th-POK , also known as ZBTB7B or cKrox ) transcription factor is a key regulator of lineage commitment of immature T cell precursors . It is yet unclear the physiological functions of Th-POK besides helper T cell differentiation . Here we show that Th-POK is restrictedly expressed in the luminal e...
Th-POK , aka cKrox or ZBTB7B , is a zinc finger transcription factor that specifies the cell fate of immature T cell precursors towards the CD4 lineage . It is yet largely unknown if Th-POK participates in the regulation of other biological processes . It is also not known if Th-POK functions beyond cell fate determina...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "milk", "reproductive", "system", "body", "fluids", "maternal", "health", "obstetrics", "and", "gynecology", "diet", "epithelial", "cells", "reproductive", "physiology", "endocrine", "physiology", "animal", "models", "model", "...
2018
Th-POK regulates mammary gland lactation through mTOR-SREBP pathway
Much of the eukaryotic genome is known to be mobile , largely due to the movement of transposons and other parasitic elements . Recent work in plants and Drosophila suggests that mobility is also a feature of many nontransposon genes and gene families . Indeed , analysis of the Arabidopsis genome suggested that as many...
Repetitive DNA , such as satellite repeats and transposons , is ubiquitous throughout the genome . Such repeats have been associated with DNA loss , circle formation , and gene transposition in plants and Drosophila . In this work we suggest that , in plants , one mechanism of gene mobility is intrachromosomal recombin...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetics", "and", "genomics", "genetics", "and", "genomics/comparative", "genomics", "evolutionary", "biology/plant", "genomes", "and", "evolution", "evolutionary", "biology/genomics" ]
2010
Transposed Genes in Arabidopsis Are Often Associated with Flanking Repeats
Natural killer ( NK ) cells and CD8+ T cells play vital roles in containing and eliminating systemic cytomegalovirus ( CMV ) . However , CMV has a tropism for the salivary gland acinar epithelial cells and persists in this organ for several weeks after primary infection . Here we characterize a distinct NK cell populat...
Human cytomegalovirus ( HCMV ) is a herpesvirus that infects 50–95% of human populations . In immunocompetent individuals , a primary infection often goes unnoticed and when resolved by the adaptive immune response , HCMV enters into a latent phase . The natural mouse pathogen murine CMV ( MCMV ) is a well-characterize...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/leukocyte", "development", "infectious", "diseases", "immunology/immune", "response", "immunology/innate", "immunity", "infectious", "diseases/viral", "infections", "immunology/immunity", "to", "infections" ]
2011
Salivary Gland NK Cells Are Phenotypically and Functionally Unique
Patients suffering from Epidermodysplasia verruciformis ( EV ) , a rare inherited skin disease , display a particular susceptibility to persistent infection with cutaneous genus beta-human papillomavirus ( beta-HPV ) , such as HPV type 8 . They have a high risk to develop non-melanoma skin cancer at sun-exposed sites ....
Cutaneous genus beta-HPV types infect skin keratinocytes . Their potential role in skin carcinogenesis , particularly in immunosuppressed patients , has become a major field of interest . Patients suffering from the rare genetic disorder Epidermodysplasia verruciformis ( EV ) are highly susceptible to persistent genus ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "transfection", "keratinocytes", "medicine", "and", "health", "sciences", "gene", "regulation", "regulatory", "proteins", "dna-binding", "proteins", "epithelial", "cells", "oncology", "protein", "expression", "transcription", "factors", "molecular", "biology", "techniques",...
2017
Identification of C/EBPα as a novel target of the HPV8 E6 protein regulating miR-203 in human keratinocytes
Mutations occur at vastly different rates across the genome , and populations , leading to differences in the spectrum of segregating polymorphisms . Here , we investigate variation in the rare variant spectrum in a sample of human genomes representing all major world populations . We find at least two distinct signatu...
Genetic variation among humans is built up by a constant stream of new mutations . New mutations appear every generation because of unrepaired DNA damage , or copying errors in DNA replication . Differences between populations in the rate or types of mutations that appear are one of several factors that affect patterns...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "demography", "ethnicities", "dna", "damage", "mutation", "epigenetics", "dna", "dna", "methylation", "chromatin", "chromosome", "biology", "native", "americans", "gene", "expression", "chromatin", "modification", "dna", "modification", "somatic", "mutation", "people", ...
2017
Differences in the rare variant spectrum among human populations
Typhoid fever is a major cause of death worldwide with a major part of the disease burden in developing regions such as the Indian sub-continent . Bangladesh is part of this highly endemic region , yet little is known about the spatial and temporal distribution of the disease at a regional scale . This research used a ...
This research studies the spatial and temporal distribution of typhoid infections in the Dhaka metropolitan area of Bangladesh in the period 2005 to 2009 . Data from hospital admission records was analysed together with a range of demographic , environmental and climatic data , in what is believed to be the first study...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion", "Conclusions" ]
[ "medicine", "public", "health", "and", "epidemiology", "spatial", "epidemiology", "geoinformatics", "global", "health", "neglected", "tropical", "diseases", "spatial", "analysis", "human", "geography", "infectious", "diseases", "geography", "environmental", "epidemiology", ...
2013
Typhoid Fever and Its Association with Environmental Factors in the Dhaka Metropolitan Area of Bangladesh: A Spatial and Time-Series Approach
Surveillance data indicate that most circulating A ( H1N1 ) pdm09 influenza viruses have remained antigenically similar since they emerged in humans in 2009 . However , antigenic drift is likely to occur in the future in response to increasing population immunity induced by infection or vaccination . In this study , se...
Infection with influenza virus leads to significant morbidity and mortality . Annual vaccination may prevent subsequent disease by inducing neutralizing antibodies to currently circulating strains in the human population . To escape this antibody response , influenza A viruses undergo continuous genetic variation as th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "influenza", "immunology", "biology", "viral", "diseases", "immune", "response" ]
2013
Antigenic Drift of the Pandemic 2009 A(H1N1) Influenza Virus in a Ferret Model
Many positive-strand RNA viruses encode genes that can function in trans , whereas other genes are required in cis for genome replication . The mechanisms underlying trans- and cis-preferences are not fully understood . Here , we evaluate this concept for hepatitis C virus ( HCV ) , an important cause of chronic liver ...
RNA viruses encode one or more proteins that form an RNA replicase , which serves to propagate the viral RNA genome . Some replication genes can function in trans , meaning that proteins expressed by one viral genome can complement and assist in the replication of a defective mutant virus within the same cell . Other v...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Hepatitis C Virus RNA Replication Depends on Specific Cis- and Trans-Acting Activities of Viral Nonstructural Proteins
Circadian rhythms are controlled by a system of negative and positive genetic feedback loops composed of clock genes . Although many genes have been implicated in these feedback loops , it is unclear whether our current list of clock genes is exhaustive . We have recently identified Chrono as a robustly cycling transcr...
The circadian clock has a fundamental role in regulating biological temporal rhythms in organisms , and it is tightly controlled by a molecular circuit consisting of positive and negative regulatory feedback loops . Although many of the clock genes comprising this circuit have been identified , there are still some cri...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "physiological", "processes", "medicine", "and", "health", "sciences", "physiology", "chronobiology", "biology", "and", "life", "sciences", "metabolism" ]
2014
A Novel Protein, CHRONO, Functions as a Core Component of the Mammalian Circadian Clock
In central Nigeria Anopheles mosquitoes transmit malaria and lymphatic filariasis ( LF ) . The strategy used for interrupting LF transmission in this area is annual mass drug administration ( MDA ) with albendazole and ivermectin , but after 8 years of MDA , entomological evaluations in sentinel villages showed continu...
In Plateau and Nasarawa states in central Nigeria , 4 million persons are threatened by a mosquito-transmitted parasitic disease called lymphatic filariasis ( LF ) . LF can lead to elephantiasis , a crippling condition in which the limbs and genitals often are grotesquely swollen or enlarged . In communities afflicted ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2013
Long-Lasting Insecticidal Nets Are Synergistic with Mass Drug Administration for Interruption of Lymphatic Filariasis Transmission in Nigeria
IFN-α/β plays a critical role in limiting viral spread , restricting viral tropism and protecting mice from neurotropic coronavirus infection . However , the IFN-α/β dependent mechanisms underlying innate anti-viral functions within the CNS are poorly understood . The role of RNase L in viral encephalomyelitis was expl...
Initial spread of viruses is controlled by type I interferon induced antiviral molecules . Early intervention with viral replication is especially critical in central nervous system infections to reduce loss of non-renewable cells and mitigate immune pathology . One of the best characterized anti-viral mechanisms is me...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/infectious", "diseases", "of", "the", "nervous", "system", "virology/host", "antiviral", "responses", "pathology/neuropathology", "immunology/innate", "immunity" ]
2009
RNase L Mediated Protection from Virus Induced Demyelination
Protein-protein interactions ( PPIs ) are essential to most fundamental cellular processes . There has been increasing interest in reconstructing PPIs networks . However , several critical difficulties exist in obtaining reliable predictions . Noticeably , false positive rates can be as high as >80% . Error correction ...
Protein interactions are the basic units in almost all biological processes . It is thus vitally important to reconstruct protein-protein interactions ( PPIs ) before we can fully understand biological processes . However , critical difficulties exist . Particularly the rate of wrongly predicting PPIs to be true ( fals...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "systems", "biology", "biochemistry", "probability", "distribution", "mathematics", "protein", "interactions", "biology", "computational", "biology", "proteomics", "bayes", "theorem", "probability", "theory" ]
2011
Bayesian Inference for Genomic Data Integration Reduces Misclassification Rate in Predicting Protein-Protein Interactions
Scabies is a disease of worldwide significance , causing considerable morbidity in both humans and other animals . The scabies mite Sarcoptes scabiei burrows into the skin of its host , obtaining nutrition from host skin and blood . Aspartic proteases mediate a range of diverse and essential physiological functions suc...
Scabies is an infectious disease of the skin caused by infestation with the parasitic mite Sarcoptes scabiei . It is a disease that has a considerable impact on humans and other animals , including livestock , wildlife and companion animals . Scabies mites burrow into the skin of their host , consuming host skin and bl...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2013
An Aspartic Protease of the Scabies Mite Sarcoptes scabiei Is Involved in the Digestion of Host Skin and Blood Macromolecules
Pooled DNA from multiple unknown organisms arises in a variety of contexts , for example microbial samples from ecological or human health research . Determining the composition of pooled samples can be difficult , especially at the scale of modern sequencing data and reference databases . Here we propose a novel metho...
Pooled DNA from multiple unknown organisms arises in a variety of contexts . Determining the composition of pooled samples can be difficult , especially at the scale of modern data . Here we propose the novel method Karp , designed to perform taxonomic profiling in pooled DNA . Karp combines the speed and low-memory re...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "taxonomy", "microbiome", "applied", "mathematics", "split-decomposition", "method", "microbiology", "simulation", "and", "modeling", "algorithms", "multiple", "alignment", "calculation", "data", "management", "mathematics", "microbial", "genomics", "research", "and", "anal...
2018
Using pseudoalignment and base quality to accurately quantify microbial community composition
Herpesviruses infect the majority of the human population and can cause significant morbidity and mortality . Herpes simplex virus ( HSV ) type 1 causes cold sores and herpes simplex keratitis , whereas HSV-2 is responsible for genital herpes . Human cytomegalovirus ( HCMV ) is the most common viral cause of congenital...
Herpesviruses are large DNA viruses that are carried by almost 100% of the adult human population . Herpesviruses include several important human pathogens , such as herpes simplex viruses ( HSV ) type 1 and 2 ( causing cold sores and genital herpes , respectively ) , human cytomegalovirus ( HCMV; the most common viral...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biotechnology", "genome", "engineering", "herpes", "simplex", "virus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "engineering", "and", "technology", "gene", "regulation", "pathogens", "synthetic", "biology", "microbiology", ...
2016
CRISPR/Cas9-Mediated Genome Editing of Herpesviruses Limits Productive and Latent Infections
Cancer is a disease driven by a combination of inherited risk alleles coupled with the acquisition of somatic mutations , including amplification and deletion of genomic DNA . Potential relationships between the inherited and somatic aspects of the disease have only rarely been examined on a genome-wide level . Applyin...
Cancer is a disease of two distinct , but related , genomes: the inherited genome and the tumor genome . Despite the fact that the tumor genome arises from the germline , the genomes are typically studied as separate entities . For example , germline genetic studies focus on how inherited variation is related to a part...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genomics", "genetics", "and", "genomics/cancer", "genetics", "computational", "biology/genomics" ]
2010
Allelic Selection of Amplicons in Glioblastoma Revealed by Combining Somatic and Germline Analysis
During the development of the spinal cord , proliferative neural progenitors differentiate into postmitotic neurons with distinct fates . How cells switch from progenitor states to differentiated fates is poorly understood . To address this question , we studied the differentiation of progenitors in the zebrafish spina...
During tissue formation , progenitor cells generate both differentiated cells and progenitor cells . It is poorly understood how this balance between self-renewal and differentiation generates the correct number of different cell types . Here , we use zebrafish spinal cord development as a model system to investigate h...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "model", "organisms", "genetics", "biology", "molecular", "cell", "biology", "neuroscience", "genetics", "and", "genomics" ]
2012
Attenuation of Notch and Hedgehog Signaling Is Required for Fate Specification in the Spinal Cord
Hepatitis C virus ( HCV ) enters hepatocytes following a complex set of receptor interactions , culminating in internalization via clathrin-mediated endocytosis . However , aside from receptors , little is known about the cellular molecular requirements for infectious HCV entry . Therefore , we analyzed a siRNA library...
Hepatitis C virus ( HCV ) chronically infects 130 million people and is a major cause of cirrhosis and liver cancer . The current antiviral therapy of pegylated interferon-2 alfa + ribavirin is successful in only half of treated patients . This has led to an intensive effort to design improved therapeutic strategies . ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/host", "invasion", "and", "cell", "entry" ]
2009
RNA Interference and Single Particle Tracking Analysis of Hepatitis C Virus Endocytosis
The reduced efficacy of current Anopheline mosquito control methods underscores the need to develop new methods of control that exploit unique target sites and/or utilizes novel deployment methods . Autodissemination methodologies using insect growth regulators ( IGRs ) is growing in interest and has been shown to be e...
Efforts to control the mosquito vector of malaria , Anopheles gambiae , have been dominated by the use of insecticide-treated bednets or residual spraying efforts for the previous 2–3 decades . The persistent use of these methods has led to a decline in control efficacy and has highlighted the need to 1 ) identify nove...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "liquid", "chromatography", "animals", "toxicology", "reproductive", "physiology", "toxicity", "developmental", "biology", "population", "biology", "i...
2018
Development of an autodissemination strategy for the deployment of novel control agents targeting the common malaria mosquito, Anopheles quadrimaculatus say (Diptera: Culicidae)
Co-localization of networks of genes in the nucleus is thought to play an important role in determining gene expression patterns . Based upon experimental data , we built a dynamical model to test whether pure diffusion could account for the observed co-localization of genes within a defined subnuclear region . A simpl...
Transcription is a fundamental step in gene expression , yet it remains poorly understood at cellular level . Textbooks are full of descriptions of promoter-bound transcription factors recruiting RNA polymerase , which initiates transcription before sliding along the transcription unit . However , increasing evidence s...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "systems", "biology", "biology", "computational", "biology", "genetics", "and", "genomics" ]
2011
A Dynamical Model Reveals Gene Co-Localizations in Nucleus
One of the most remarkable features of the human brain is its ability to adapt rapidly and efficiently to external task demands . Novel and non-routine tasks , for example , are implemented faster than structural connections can be formed . The neural underpinnings of these dynamics are far from understood . Here we de...
As we go about the day , our brains must quickly adapt in accordance with our internal goals . These modifications generally occur within the constraints of a fixed structural architecture , thus manifesting as rapid changes in the recruitment of and integration between brain regions conforming to task demands . For ex...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
A Functional Cartography of Cognitive Systems
Spinster homolog 2 ( Spns2 ) acts as a Sphingosine-1-phosphate ( S1P ) transporter in zebrafish and mice , regulating heart development and lymphocyte trafficking respectively . S1P is a biologically active lysophospholipid with multiple roles in signalling . The mechanism of action of Spns2 is still elusive in mammals...
Progressive hearing loss is common in the human population but we know very little about the molecular mechanisms involved . Mutant mice are useful for investigating these mechanisms and have revealed a wide range of different abnormalities that can all lead to the same outcome: deafness . We report here our findings o...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "genetics", "phenotypes", "physiology", "genetics", "biology", "and", "life", "sciences", "sensory", "perception", "electrophysiology", "mammalian", "genetics", "neuroscience", "hearing" ]
2014
Spinster Homolog 2 (Spns2) Deficiency Causes Early Onset Progressive Hearing Loss
Recent studies have greatly increased understanding of how the immune system of insects responds to infection , whereas much less is known about how pathogens subvert immune defenses . Key regulators of the insect immune system are Rel proteins that form Nuclear Factor-κB ( NF-κB ) transcription factors , and inhibitor...
Central to the study of host-pathogen interactions is understanding how the immune system of hosts responds to infection , and reciprocally how pathogens subvert host defenses . In the case of insects , understanding of how the immune system responds to infection greatly exceeds understanding of pathogen counterstrateg...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "dna", "viruses", "virology", "viral", "classification", "biology", "microbiology", "parasitology", "parasite", "physiology" ]
2012
Polydnavirus Ank Proteins Bind NF-κB Homodimers and Inhibit Processing of Relish
We present a hidden Markov model ( HMM ) for inferring gradual isolation between two populations during speciation , modelled as a time interval with restricted gene flow . The HMM describes the history of adjacent nucleotides in two genomic sequences , such that the nucleotides can be separated by recombination , can ...
Next-generation sequencing technology has enabled the generation of whole-genome data for many closely related species . For population genetic inference we have sequenced many loci , but only in a few individuals . We present a new method that allows inference of the divergence process based on two closely related gen...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genome", "evolution", "population", "genetics", "genome", "analysis", "tools", "effective", "population", "size", "speciation", "population", "biology", "biology", "evolutionary", "genetics", "genetics", "gene", "flow", "genomics", "evolutionary", "biology", "genomic", ...
2012
A New Isolation with Migration Model along Complete Genomes Infers Very Different Divergence Processes among Closely Related Great Ape Species
Following DNA damage or replication stress , budding yeast cells activate the Rad53 checkpoint kinase , promoting genome stability in these challenging conditions . The DNA damage and replication checkpoint pathways are partially overlapping , sharing several factors , but are also differentiated at various levels . Th...
The maintenance of genome stability is an essential process which needs a careful control . Indeed , the checkpoints are surveillance mechanisms sensing alterations in the integrity of the genome and preventing the replication and segregation of defective DNA molecules . The DNA integrity checkpoint is a signal transdu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cellular", "stress", "responses", "cell", "biology", "nucleic", "acids", "genetics", "dna", "biology", "molecular", "cell", "biology", "dna", "repair", "genetics", "and", "genomics", "molecular", "biology", "dna", "synthesis" ]
2011
Sensing of Replication Stress and Mec1 Activation Act through Two Independent Pathways Involving the 9-1-1 Complex and DNA Polymerase ε
Venezuelan equine encephalitis virus ( VEEV ) has been responsible for hundreds of thousands of human and equine cases of severe disease in the Americas . A passive surveillance study was conducted in Peru , Bolivia and Ecuador to determine the arboviral etiology of febrile illness . Patients with suspected viral-assoc...
Venezuelan equine encephalitis virus ( VEEV ) has been responsible for hundreds of thousands of human and equine cases of severe disease in the Americas . In 2005–2007 , cases of Venezuelan equine encephalitis ( VEE ) were diagnosed for the first time in residents of Bolivia; the patients did not report traveling , sug...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "virology/emerging", "viral", "diseases", "virology" ]
2009
Genetic Characterization of Venezuelan Equine Encephalitis Virus from Bolivia, Ecuador and Peru: Identification of a New Subtype ID Lineage
Chlamydophila psittaci is found worldwide , but is particularly common among psittacine birds in tropical and subtropical regions . While investigating a human psittacosis outbreak that was associated with avian chlamydiosis in Hong Kong , we identified a novel adenovirus in epidemiologically linked Mealy Parrots , whi...
Chlamydophila psittaci is a bacterial pathogen which can cause avian chlamydiosis and human psittacosis . Although C . psittaci is frequently detected in birds from the tropical and subtropical regions , large outbreaks of human infections rarely occur . During the investigation of a human psittacosis outbreak that was...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "biology", "and", "life", "sciences", "microbiology", "medicine", "and", "health", "sciences" ]
2014
A Novel Psittacine Adenovirus Identified During an Outbreak of Avian Chlamydiosis and Human Psittacosis: Zoonosis Associated with Virus-Bacterium Coinfection in Birds
We are reporting qualitative and quantitative changes of the extracellular matrix ( ECM ) and associated receptor proteomes , occurring during the transition from liver fibrosis and steatohepatitis to hepatocellular carcinoma ( HCC ) . We compared two mouse models relevant to human HCC: PDGFC transgenic ( Tg ) and Pten...
The microenvironment can have a profound influence on cellular behavior and survival and on growth of developing tumor cells . We present the first comprehensive analysis of the extracellular matrix ( ECM ) and associated receptor proteomes , applied here to the study of hepatocellular carcinoma ( HCC ) . This study de...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "cancer", "detection", "and", "diagnosis", "medicine", "cancers", "and", "neoplasms", "basic", "cancer", "research", "gastrointestinal", "tumors", "liver", "diseases", "animal", "models", "oncology", "model", "organisms", "gastroenterology", "and", "hepatology", "proteo...
2011
Extracellular Matrix Dynamics in Hepatocarcinogenesis: a Comparative Proteomics Study of PDGFC Transgenic and Pten Null Mouse Models
It has recently been noted that the relative prevalence of the various kinds of epistasis varies along an adaptive walk . This has been explained as a result of mean regression in NK model fitness landscapes . Here we show that this phenomenon occurs quite generally in fitness landscapes . We propose a simple and gener...
The main result concerns the changing geometry along an adaptive walk in a fitness landscape . An adaptive walk is described by a sequence of genotypes of increasing fitness , where two consecutive genotypes differ by a point mutation . We compare patterns of epistasis , or gene interactions , along adaptive walks . Ro...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mathematics", "biology", "and", "life", "sciences", "physical", "sciences" ]
2014
The Changing Geometry of a Fitness Landscape Along an Adaptive Walk
Clostridium difficile , a Gram positive , anaerobic , spore-forming bacterium is an emergent pathogen and the most common cause of nosocomial diarrhea . Although transmission of C . difficile is mediated by contamination of the gut by spores , the regulatory cascade controlling spore formation remains poorly characteri...
Clostridium difficile , a major cause of antibiotic-associated diarrhea , produces resistant spores that facilitate the persistence of this bacterium in the environment including hospitals . Its transmission is mediated by contamination of gut by spores . Understanding how this complex developmental process is regulate...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Genome-Wide Analysis of Cell Type-Specific Gene Transcription during Spore Formation in Clostridium difficile
Iron is essential for photosynthesis and is often a limiting nutrient for plant productivity . Plants respond to conditions of iron deficiency by increasing transcript abundance of key genes involved in iron homeostasis , but only a few regulators of these genes have been identified . Using genome-wide expression analy...
Iron deficiency is the most common human nutritional disorder , afflicting more than three billion people worldwide . Most of these people rely on plants for their dietary iron . Iron is also one of the three nutrients that most commonly limit plant growth . Despite progress in tracing how Fe moves throughout the plant...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
MYB10 and MYB72 Are Required for Growth under Iron-Limiting Conditions
A major tenet in theoretical neuroscience is that cognitive and behavioral processes are ultimately implemented in terms of the neural system dynamics . Accordingly , a major aim for the analysis of neurophysiological measurements should lie in the identification of the computational dynamics underlying task processing...
Computational processes in the brain are often assumed to be implemented in terms of nonlinear neural network dynamics . However , experimentally we usually do not have direct access to this underlying dynamical process that generated the observed time series , but have to infer it from a sample of noisy and mixed meas...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "diagnostic", "radiology", "functional", "magnetic", "resonance", "imaging", "applied", "mathematics", "random", "variables", "neuroscience", "covariance", "magnetic", "resonance", "imaging", "algorithms", "simulation", "and", "mod...
2019
Identifying nonlinear dynamical systems via generative recurrent neural networks with applications to fMRI
Unlike most other fungi , molds of the genus Trichoderma ( Hypocreales , Ascomycota ) are aggressive parasites of other fungi and efficient decomposers of plant biomass . Although nutritional shifts are common among hypocrealean fungi , there are no examples of such broad substrate versatility as that observed in Trich...
Individual fungi rely on particular host organisms or substrates for their nutrition . Therefore , the genomes of fungi feeding on plant biomass necessarily contain genes encoding plant cell wall-degrading enzymes , while animal parasites may depend on proteolytic activity . Molds in the genus Trichoderma ( Ascomycota ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "taxonomy", "fungal", "genetics", "parasite", "evolution", "parasitology", "fungi", "phylogenetics", "data", "management", "phylogenetic", "analysis", "fungal", "evolution", "mycology", "computer", "and", "information", "sciences", "ascomycetes", "evolutionary", "systematic...
2018
Massive lateral transfer of genes encoding plant cell wall-degrading enzymes to the mycoparasitic fungus Trichoderma from its plant-associated hosts
Sex ratio distortion ( sex-ratio for short ) has been reported in numerous species such as Drosophila , where distortion can readily be detected in experimental crosses , but the molecular mechanisms remain elusive . Here we characterize an autosomal sex-ratio suppressor from D . simulans that we designate as not much ...
Genetic conflicts among genes happen when their modes of transmission differ . Genes in the heterogametic ( XY ) sex can be grouped as X-linked , Y-linked , autosomal , or cytoplasmic . Sex ratio in the progeny greatly affects the transmission advantage of each of the four types of genes , with the optimal sex ratio fo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "developmental", "biology", "evolutionary", "biology", "molecular", "biology", "genetics", "and", "genomics" ]
2007
A sex-ratio Meiotic Drive System in Drosophila simulans. I: An Autosomal Suppressor
Cryptococcus neoformans is a pathogenic basidiomycetous yeast responsible for more than 600 , 000 deaths each year . It occurs as two serotypes ( A and D ) representing two varieties ( i . e . grubii and neoformans , respectively ) . Here , we sequenced the genome and performed an RNA-Seq-based analysis of the C . neof...
Cryptococcus neoformans var . grubii is a major human pathogen responsible for deadly meningoencephalitis in immunocompromised patients . Here , we report the sequencing and annotation of its genome . Evidence for extensive intron splicing , antisense transcription , non-coding RNAs , and alternative polyadenylation in...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "sequencing", "techniques", "genome", "expression", "analysis", "organismal", "evolution", "genome", "evolution", "microbiology", "genome", "sequencing", "fungal", "evolution", "genome", "analysis", "microbial", "evolution", "molecular", "biology", "techniq...
2014
Analysis of the Genome and Transcriptome of Cryptococcus neoformans var. grubii Reveals Complex RNA Expression and Microevolution Leading to Virulence Attenuation
MicroRNAs ( miRNAs ) inhibit expression of target genes by binding to their RNA transcripts . It has been recently shown that RNA transcripts targeted by the same miRNA could “compete” for the miRNA molecules and thereby indirectly regulate each other . Experimental evidence has suggested that the aberration of such mi...
CeRNA interaction is a post-transcriptional gene regulation that involves interactions between RNAs competing for common miRNA regulators . Dysregulation of ceRNA interactions have been implicated in multiple diseases including cancer . Here we propose a computational pipeline called Cancerin that infers genome-wide ce...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "gene", "regulation", "cancer", "risk", "factors", "long", "non-coding", "rnas", "oncology", "regulator", "genes", "micrornas", "gene", "types", "epigenetics", "dna", "chromatin", "dna", "methylation", "research", "and", "ana...
2018
Cancerin: A computational pipeline to infer cancer-associated ceRNA interaction networks
Since the first discovery of deep-sea hydrothermal vents along the Galápagos Rift in 1977 , numerous vent sites and endemic faunal assemblages have been found along mid-ocean ridges and back-arc basins at low to mid latitudes . These discoveries have suggested the existence of separate biogeographic provinces in the At...
Deep-sea hydrothermal vents are mainly associated with seafloor spreading at mid-ocean ridges and in basins near volcanic island arcs . They host animals found nowhere else that derive their energy not from the sun but from bacterial oxidation of chemicals in the vent fluids , particularly hydrogen sulphide . Hydrother...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biogeography", "plate", "tectonics", "evolutionary", "ecology", "astrobiology", "biota", "extremophiles", "microbiology", "biodiversity", "oceans", "marine", "biology", "animal", "phylogenetics", "biogeochemistry", "marine", "technology", "geology", "spreading", "centers", ...
2012
The Discovery of New Deep-Sea Hydrothermal Vent Communities in the Southern Ocean and Implications for Biogeography
Combinatorial gene perturbations provide rich information for a systematic exploration of genetic interactions . Despite successful applications to bacteria and yeast , the scalability of this approach remains a major challenge for higher organisms such as humans . Here , we report a novel experimental and computationa...
Synthetic genetic interactions estimated from combinatorial gene perturbation screens provide systematic insights into synergistic interactions of genes in a biological process . However , this approach lacks scalability for large-scale genetic interaction profiling in metazoan organisms such as humans . We contribute ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "systems", "biology", "functional", "genomics", "biology", "genomics", "genetics", "and", "genomics" ]
2012
Posterior Association Networks and Functional Modules Inferred from Rich Phenotypes of Gene Perturbations
Acute kidney injury ( AKI ) is a major complication of snake envenoming , but early diagnosis remains problematic . We aimed to investigate the time course of novel renal biomarkers in AKI following Russell’s viper ( Daboia russelii ) bites . We recruited a cohort of patients with definite Russell’s viper envenoming an...
Snake envenoming is a neglected tropical disease that causes death and severe effects in the rural tropics . Acute kidney injury is a major cause of morbidity in snake envenoming , but continues to be poorly recognised and early diagnosis is difficult . We aimed to investigate the time course of renal biomarkers in acu...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "toxins", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "vertebrates", "biomarkers", "animals", "toxicology", "toxic", "agents", "urine", "reptiles", "neglected", "tropical", "diseases", ...
2019
Early identification of acute kidney injury in Russell’s viper (Daboia russelii) envenoming using renal biomarkers
Identifying naturally-occurring neutralizing antibodies ( NAb ) that are cross-reactive against all global subtypes of HIV-1 is an important step toward the development of a vaccine . Establishing the host and viral determinants for eliciting such broadly NAbs is also critical for immunogen design . NAb breadth has pre...
A broad and potent antibody response is considered essential for an effective HIV-1 vaccine that will protect against diverse circulating strains . Consequently , there is great interest in both the host and viral factors that impact the development of the neutralizing antibody ( NAb ) response in natural HIV-1 infecti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunity", "virology", "immunology", "biology", "microbiology" ]
2012
HIV-1 Superinfection in Women Broadens and Strengthens the Neutralizing Antibody Response
Chikungunya virus ( CHIKV ) is an emerging mosquito-borne pathogen circulating in tropical and sub-tropical regions . Although autochthonous transmission has not been reported in Australia , there is a potential risk of local CHIKV outbreaks due to the presence of suitable vectors , global trade , frequent internationa...
Chikungunya virus ( CHIKV ) is mosquito-borne virus circulating in tropical and sub-tropical areas of the globe . Infected travellers from CHIKV-affected areas can initiate outbreaks and epidemics in countries where vector mosquitoes are present . Greater understanding of the pattern of imported cases is required to fa...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "togaviruses", "chikungunya", "infection", "atmospheric", "science", "pathogens", "population", "dynamics", "tropical", "diseases", "microbiology", "geographical", "locat...
2019
El Niño Southern Oscillation, overseas arrivals and imported chikungunya cases in Australia: A time series analysis
Receptor tyrosine kinases ( RTKs ) and Notch ( N ) proteins are different types of transmembrane receptors that transduce extracellular signals and control cell fate . Here we examine cell fate specification in the Drosophila retina and ask how N acts together with the RTKs Sevenless ( Sev ) and the EGF receptor ( DER ...
Cells are often directed to their developmental fates by the signals they receive from other cells . The Drosophila eye has become a classic paradigm for studying such signaling , and in this system direct neighbor-to-neighbor signaling plays a large role . The R7 photoreceptor is directed to its fate by signals derive...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2011
Three Distinct Roles for Notch in Drosophila R7 Photoreceptor Specification
Traditional Chinese medicine ( TCM ) has been practiced for thousands of years , but only within the last few decades has its use become more widespread outside of Asia . Concerns continue to be raised about the efficacy , legality , and safety of many popular complementary alternative medicines , including TCMs . Ingr...
Chemicals derived from plants and animals are widely used in traditional Chinese medicine ( TCM ) , and it is commonplace for remedies to contain a complex list of ingredients . Due to their heterogeneous origins , and subsequent processing into pills and powders , it can be difficult for the biological origin of ingre...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "medicine", "animal", "genetics", "complementary", "and", "alternative", "medicine", "genetics", "plant", "genetics", "molecular", "genetics", "biology", "genetics", "and", "genomics" ]
2012
Deep Sequencing of Plant and Animal DNA Contained within Traditional Chinese Medicines Reveals Legality Issues and Health Safety Concerns
We propose a novel method for detecting sites of molecular recombination in multiple alignments . Our approach is a compromise between previous extremes of computationally prohibitive but mathematically rigorous methods and imprecise heuristic methods . Using a combined algorithm for estimating tree structure and hidde...
In viral and bacterial pathogens , recombination has the ability to combine fitness-enhancing mutations . Accurate characterization of recombinant breakpoints in newly sequenced strains can provide information about the role of this process in evolution , for example , in immune evasion . Of particular interest are sit...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/evolutionary", "modeling", "computational", "biology/genomics" ]
2009
Accurate Detection of Recombinant Breakpoints in Whole-Genome Alignments
Hookworms survive for several years ( 5 to 7 years ) in the host lumen , inducing a robust but largely ineffective immune response . Among the most striking aspects of the immune response to hookworm ( as with many other helminths ) is the ablation of parasite-specific T cell proliferative response ( hyporesponsiveness...
Hookworms survive for several years in the host lumen , inducing a robust but ineffective immune response . While the role of the adaptive response in human helminth infection has been well investigated , the role of the innate immune responses remains to be elucidated . We report on the development of dendritic cells ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "immunology/immunomodulation", "infectious", "diseases/helminth", "infections", "immunology/immunity", "to", "infections", "immunology/innate", "immunity" ]
2009
Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
Altruistic behavior is considered a key feature of the human cooperative makeup , with deep ontogenetic roots . The tendency to engage in altruistic behavior varies between individuals and has been linked to differences in responding to fearful faces . The current study tests the hypothesis that this link exists from e...
Altruistic behavior such as helping an unfamiliar person in need is considered a key feature of cooperation in human societies . Yet our propensity to engage in altruistic acts varies considerably among individuals , ranging from extraordinarily altruistic kidney donors to highly antisocial psychopaths . We examined th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "children", "medicine", "and", "health", "sciences", "face", "social", "sciences", "neuroscience", "age", "groups", "fear", "cognitive", "psychology", "infants", "altruistic", "behavior", "eyes", "families", "social", "cognition", "emotions", "prosocial", "behavior", ...
2018
The neurodevelopmental precursors of altruistic behavior in infancy
Protozoan parasites , such as Leishmania , still pose an enormous public health problem in many countries throughout the world . Current measures are outdated and have some associated drug resistance , prompting the search into novel therapies . Several innovative approaches are under investigation , including the util...
Protozoan parasites are the causative agent of much disease in tropical areas of the world . Currently , the control of these diseases is dependent on outdated drug treatment , with associated high toxicity and drug resistance . There is an urgent need for novel anti-parasitic therapies . One emerging anti-parasitic th...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "leishmaniasis", "neglected", "tropical", "diseases", "biology", "microbiology" ]
2011
Effect of BMAP-28 Antimicrobial Peptides on Leishmania major Promastigote and Amastigote Growth: Role of Leishmanolysin in Parasite Survival
The occurrences of many environmentally-persistent and zoonotic infections are driven by ecosystem changes , which in turn are underpinned by land-use modifications that alter the governance of pathogen , biodiversity and human interactions . Our current understanding of these ecological changes on disease emergence ho...
This study provides the first ever recorded extraction of Mycobacterium ulcerans DNA from the environment in South America , specifically from French Guiana an ultra-peripheral French territory . M . ulcerans is the causative agent responsible for the devastating necrotic skin infection Buruli ulcer , which is prevalen...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "ecological", "environments", "freshwater", "environments", "aquatic", "environments", "ecology", "emerging", "infectious", "diseases", "genetics", "microbial", "pathogens", "molecular", "genetics", "biology", "microbiology", "freshwater", "ecology", "genetics", "of", "dise...
2014
First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America
Dicer is a key component of RNA interference ( RNAi ) and well-known for its role in biogenesis of micro ( mi ) RNA in the cytoplasm . Increasing evidence suggests that mammalian Dicer is also present and active in the nucleus . We have previously shown that phosphorylated human Dicer associates with chromatin in respo...
Cytoplasmic Dicer is a key component of the canonical micro ( mi ) RNA biogenesis pathway . However , a growing body of evidence points toward localisation and activity of mammalian Dicer in the nucleus . A recent study by Much et al . , employed an endogenously HA-tagged Dicer knock-in mouse cell line to show that Dic...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "nuclear", "staining", "molecular", "probe", "techniques", "immunoblotting", "cytoplasmic", "staining", "dna", "damage", "immunoprecipitation", "dna", "molecular", "biology", "techniques", "cellular", "structures", "and", "organelles", "research", "and", ...
2018
Nuclear re-localization of Dicer in primary mouse embryonic fibroblast nuclei following DNA damage
Eumycetoma is a chronic infectious disease characterized by a large subcutaneous mass , often caused by the fungus Madurella mycetomatis . A combination of surgery and prolonged medication is needed to treat this infection with a success rate of only 30% . There is , therefore , an urgent need to find more effective dr...
Mycetoma is a poverty-associated disease that was recently recognised as a neglected tropical disease by the World Health Organisation ( WHO ) . This disease can be caused by either bacteria ( actinomycetoma ) or fungi ( eumycetoma ) . The most common causative agent of mycetoma is the fungus Madurella mycetomatis . Ac...
[ "Abstract", "Introduction", "Material", "and", "methods", "Results", "Discussion" ]
[ "antimicrobials", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "mycetoma", "pathogens", "drugs", "tropical", "diseases", "microbiology", "antifungals", "developmental", "biology", "fungi", "neglected", "tropical", "diseases", "pha...
2018
Addressing the most neglected diseases through an open research model: The discovery of fenarimols as novel drug candidates for eumycetoma
A key challenge in the production of second generation biofuels is the conversion of lignocellulosic substrates into fermentable sugars . Enzymes , particularly those from fungi , are a central part of this process , and many have been isolated and characterised . However , relatively little is known of how fungi respo...
The aim of second generation biofuels is to produce fuels from non-food crops and agricultural wastes such as straw . A key , and often limiting , step is the extraction of simple sugars ( saccharification ) from the complex plant materials . This typically requires the use of fungal enzymes . Many such enzymes have be...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "biotechnology", "genome", "expression", "analysis", "microbiology", "fungal", "physiology", "genome", "analysis", "tools", "fungi", "applied", "microbiology", "environmental", "biotechnology", "mycology", "biodegradation", "biology", "transcriptomes", "genomics", "computatio...
2012
Uncovering the Genome-Wide Transcriptional Responses of the Filamentous Fungus Aspergillus niger to Lignocellulose Using RNA Sequencing
Elevated aerobic glycolysis in cancer cells ( the Warburg effect ) may be attributed to respiration injury or mitochondrial dysfunction , but the underlying mechanisms and therapeutic significance remain elusive . Here we report that induction of mitochondrial respiratory defect by tetracycline-controlled expression of...
Glycolysis is a cytoplasmic metabolic process that produces energy from glucose . In normal cells , the rate of glycolysis is low , and glycolysis products are further processed in the mitochondria via oxidative phosphorylation , a very efficient energy-producing process . Cancer cells , however , display higher levels...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "biology" ]
2012
Novel Role of NOX in Supporting Aerobic Glycolysis in Cancer Cells with Mitochondrial Dysfunction and as a Potential Target for Cancer Therapy
Telomere capture , a rare event that stabilizes chromosome breaks , is associated with certain genetic abnormalities in humans . Studies pertaining to the generation , maintenance , and biological effects of telomere formation are limited in metazoans . A mutation , mu2a , in Drosophila melanogaster decreases the rate ...
Telomeres are structures at the ends of eukaryotic chromosomes required for chromosome stability . If unrepaired , a single chromosome end without a telomere is sufficient to kill a cell , but new telomere formation is rare . Previously , we described a gene in Drosophila whose mutants , after irradiation , produced ma...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/chromosome", "biology", "cell", "biology/cellular", "death", "and", "stress", "responses" ]
2009
Recognition of Double Strand Breaks by a Mutator Protein (MU2) in Drosophila melanogaster
Bats are recognized as a major reservoir of lyssaviruses; however , no bat lyssavirus has been isolated in Asia except for Aravan and Khujand virus in Central Asia . All Chinese lyssavirus isolates in previous reports have been of species rabies virus , mainly from dogs . Following at least two recent bat-associated hu...
The Lyssavirus genus presently comprises 12 species and two unapproved species with different antigenic characteristics . Rabies virus is detectable worldwide; Lagos bat virus , Mokola virus , Duvenhage virus , Shimoni bat virus , and Ikoma lyssavirus circulate in Africa; European bat lyssavirus types 1 and 2 , Irkut v...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "public", "health", "and", "epidemiology", "veterinary", "epidemiology", "veterinary", "microbiology", "epidemiology", "microbiology", "veterinary", "science" ]
2013
Isolation of Irkut Virus from a Murina leucogaster Bat in China
For patients infected with hepatitis C virus ( HCV ) , the combination of the direct-acting antiviral agent telaprevir , pegylated-interferon alfa ( Peg-IFN ) , and ribavirin ( RBV ) significantly increases the chances of sustained virologic response ( SVR ) over treatment with Peg-IFN and RBV alone . If patients do no...
Hepatitis C virus ( HCV ) chronically infects approximately 170 million people worldwide . The goal of HCV treatment is viral eradication ( sustained virologic response; SVR ) . Telaprevir directly inhibits viral replication by inhibiting the HCV protease , leading to high SVR rates when combined with pegylated-interfe...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biotechnology", "antimicrobials", "organismal", "evolution", "medicine", "and", "health", "sciences", "population", "dynamics", "applied", "mathematics", "microbiology", "liver", "diseases", "algorithms", "infectious", "hepatitis", "systems", "science", "mathematics", "gas...
2014
Modeling Viral Evolutionary Dynamics after Telaprevir-Based Treatment
In the Niayes area , located in the west of Senegal , only one tsetse species , Glossina palpalis gambiensis Vanderplank ( Diptera: Glossinidae ) was present . The Government of Senegal initiated and implemented an elimination programme in this area that included a sterile insect technique ( SIT ) component . The G . p...
Senegal is one of the many African countries infested by tsetse flies responsible for the transmission of trypanosomes to humans and animals , causing health and economic losses . In the Niayes area , located in the west of Senegal , only one tsetse species , Glossina palpalis gambiensis Vanderplank ( Diptera: Glossini...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "death", "rates", "invertebrates", "ecology", "and", "environmental", "sciences", "geographical", "locations", "parasitic", "protozoans", "animals", "glossina", "developmental", "biology", "protozoans", "pupae", "population", "biology", "africa", "senegal", "ecosystems", ...
2017
Competitiveness and survival of two strains of Glossina palpalis gambiensis in an urban area of Senegal
Nematode parasites cause substantial morbidity to billions of people and considerable losses in livestock and food crops . The repertoire of effective anthelmintic compounds for treating these parasitoses is very limited , as drug development has been delayed for decades . Moreover , resistance has become a global conc...
Intestinal helminth infections affect approximately one-third of the world’s population , particularly in developing countries . Paradoxically , drug development in this area has been delayed for years . In addition , resistance to currently available drugs is also an emerging global concern . Therefore , there is an u...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "bacteriology", "invertebrates", "medicine", "and", "health", "sciences", "chemical", "compounds", "pathology", "and", "laboratory", "medicine", "caenorhabditis", "microbiology", "animals", "organic", "compounds", "animal", "models", "developmental", "biology", "caenorhabdi...
2018
Diisopropylphenyl-imidazole (DII): A new compound that exerts anthelmintic activity through novel molecular mechanisms
In Saccharomyces cerevisiae , splicing is critical for expression of ribosomal protein genes ( RPGs ) , which are among the most highly expressed genes and are tightly regulated according to growth and environmental conditions . However , knowledge of the precise mechanisms by which RPG pre-mRNA splicing is regulated o...
Ribosomes are responsible for protein production in all living cells , serving as the grounds for the translation of genetic information from RNA to protein . Given the vital role of the ribosome in protein assembly , ribosome components are highly expressed and are subject to tight regulation . Some ribosomal proteins...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "reverse", "transcriptase-polymerase", "chain", "reaction", "molecular", "probe", "techniques", "rna", "extraction", "northern", "blot", "fungi", "molecular", "biology", "techniques", "gel", "electrophoresis", "extraction", "techniques", "cellular", "structures", "and", "...
2016
Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae
The endosymbiont Wolbachia is known to block replication of several important arboviruses , including dengue virus ( DENV ) , in the mosquito vector Aedes aegypti . So far , the exact mechanism of this viral inhibition is not fully understood . A recent study in Drosophila melanogaster has demonstrated an interaction b...
Dengue infection along with its related disease conditions poses a significant threat to human populations . The pathogen responsible for this infection is dengue virus ( DENV ) , which is primarily transmitted to humans through the bites of Ae . aegypti mosquitoes . Unavailability of vaccines has recently sparked rese...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "dengue", "virus", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "microbiology", "animals", "wolbachia", "animal", "models", "viruses", "micrornas", "model", "organisms", "droso...
2018
Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti
Despite doubts about methods used and the association between vector density and dengue transmission , routine sampling of mosquito vector populations is common in dengue-endemic countries worldwide . This study examined the evidence from published studies for the existence of any quantitative relationship between vect...
Routine sampling of mosquito vector populations is common in dengue-endemic countries worldwide despite doubts about methods used or the correlation between vector density and dengue transmission . This systematic review examined the published evidence investigating associations between vector indices and dengue cases ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "medicine", "and", "health", "sciences", "dengue", "fever", "neglected", "tropical", "diseases", "tropical", "diseases", "viral", "diseases", "infectious", "disease", "control" ]
2014
Assessing the Relationship between Vector Indices and Dengue Transmission: A Systematic Review of the Evidence
We present the AGEMAP ( Atlas of Gene Expression in Mouse Aging Project ) gene expression database , which is a resource that catalogs changes in gene expression as a function of age in mice . The AGEMAP database includes expression changes for 8 , 932 genes in 16 tissues as a function of age . We found great heterogen...
This work studies the aging process in mice using DNA microarrays to identify genes that change expression during aging . The entire set of age-regulated genes constitutes a transcriptional profile that can be used to measure ages of different individuals . Furthermore , the aging expression profile highlights genetic ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "caenorhabditis", "mus", "(mouse)", "drosophila", "homo", "(human)", "genetics", "and", "genomics" ]
2007
AGEMAP: A Gene Expression Database for Aging in Mice
The remarkable maneuverability of flying animals results from precise movements of their highly specialized wings . Bats have evolved an impressive capacity to control their flight , in large part due to their ability to modulate wing shape , area , and angle of attack through many independently controlled joints . Bat...
Bats demonstrate remarkable agility in flight , reorienting from a horizontal flying position to a heels-over-head roosting position and recovering from aerial stumbles with ease . In this paper , we demonstrate that bats are able to execute these elegant maneuvers using primarily inertial forces , by controlled articu...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "and", "Discussion", "Conclusions" ]
[]
2015
Falling with Style: Bats Perform Complex Aerial Rotations by Adjusting Wing Inertia
Type IV pili are polymeric fibers which protrude from the cell surface and play a critical role in adhesion and invasion by pathogenic bacteria . The secretion of pili across the periplasm and outer membrane is mediated by a specialized secretin protein , PilQ , but the way in which this large channel is formed is unkn...
Many bacteria which cause infectious disease in humans use large fibers , called pili , to attach to the surfaces of the cells of the host . Pili are also involved in a particular type of movement of bacteria , termed twitching motility , and the uptake of DNA into the bacterial cell . They are made up of thousands of ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "gram", "negative", "proteins", "macromolecular", "assemblies", "biology", "microbiology", "bacterial", "pathogens" ]
2012
Structure and Assembly of a Trans-Periplasmic Channel for Type IV Pili in Neisseria meningitidis
The cytosolic glutathione transferase ( cytGST ) superfamily comprises more than 13 , 000 nonredundant sequences found throughout the biosphere . Their key roles in metabolism and defense against oxidative damage have led to thousands of studies over several decades . Despite this attention , little is known about the ...
Cytosolic glutathione transferases ( cytGSTs ) are a large and diverse superfamily of enzymes that have important roles in metabolism and defense against oxidative damage . They have been studied for several decades but because of the synthetic nature of the chemicals used to test these proteins to determine if they ha...
[ "Abstract", "Introduction", "Methods", "Results", "and", "Discussion" ]
[ "biochemistry", "biological", "data", "management", "proteins", "enzymes", "biology", "and", "life", "sciences", "enzymology", "computational", "biology" ]
2014
Large-Scale Determination of Sequence, Structure, and Function Relationships in Cytosolic Glutathione Transferases across the Biosphere
Damage initiates a pleiotropic cellular response aimed at cellular survival when appropriate . To identify genes required for damage survival , we used a cell-based RNAi screen against the Drosophila genome and the alkylating agent methyl methanesulphonate ( MMS ) . Similar studies performed in other model organisms re...
Cellular damage is known to elicit a pleiotropic response , but the relative importance of the constituent components in cell survival is poorly understood . To provide an unbiased identification of the proteins utilized in damage survival , we performed an RNAi survival screen in fly cells with methyl methanesulfonate...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetics", "and", "genomics/genomics", "genetics", "and", "genomics/comparative", "genomics", "genetics", "and", "genomics/functional", "genomics", "genetics", "and", "genomics", "genetics", "and", "genomics/bioinformatics" ]
2009
A Network of Conserved Damage Survival Pathways Revealed by a Genomic RNAi Screen
A precise and rapid adjustment of fluxes through metabolic pathways is crucial for organisms to prevail in changing environmental conditions . Based on this reasoning , many guiding principles that govern the evolution of metabolic networks and their regulation have been uncovered . To this end , methods from dynamic o...
Understanding the guiding principles behind the evolution of metabolic networks and their regulation is of fundamental importance in a broad range of disciplines reaching from the identification of novel targets to treat infections to the utilization of microbial organisms in biotechnological production processes . In ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "enzymes", "gene", "regulation", "enzymology", "toxicology", "optimization", "toxicity", "mathematics", "enzyme", "metabolism", "enzyme", "kinetics", "enzyme", "chemistry", "proteins", ...
2017
Optimality principles reveal a complex interplay of intermediate toxicity and kinetic efficiency in the regulation of prokaryotic metabolism