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The p53 family activates many of the same genes in response to DNA damage . Because p63 and p73 have structural differences from p53 and play distinct biological functions in development and metastasis , it is likely that they activate a unique transcriptional network . Therefore , we performed a genome-wide analysis u...
p63 and p73 have been identified as important suppressors of tumorigenesis and metastasis . Although they are structurally similar to p53 , they have many functions that are unique including roles in development and metastasis . Here we show , using a genome-wide analysis of cells lacking p63 and p73 individually and i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/animal", "genetics", "genetics", "and", "genomics/cancer", "genetics", "genetics", "and", "genomics/bioinformatics" ]
2009
p63 and p73 Transcriptionally Regulate Genes Involved in DNA Repair
Accurate chromosome segregation during meiosis requires that homologous chromosomes pair and become physically connected so that they can orient properly on the meiosis I spindle . These connections are formed by homologous recombination closely integrated with the development of meiosis-specific , higher-order chromos...
Meiosis is the specialized cell division that gives rise to reproductive cells such as sperm and eggs . During meiosis in most organisms , genetic information is exchanged between homologous maternal and paternal chromosomes through the process of homologous recombination . This recombination forms connections between ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology/germ", "cells", "genetics", "and", "genomics/nuclear", "structure", "and", "function", "genetics", "and", "genomics/chromosome", "biology", "biochemistry/replication", "and", "repair", "genetics", "and", "genomics/gene", "function" ]
2010
Mouse TRIP13/PCH2 Is Required for Recombination and Normal Higher-Order Chromosome Structure during Meiosis
The parameters that modulate the functional capacity of secondary Th1 effector cells are poorly understood . In this study , we employ a serial adoptive transfer model system to show that the functional differentiation and secondary memory potential of secondary CD4+ effector T cells are dependent on the inflammatory e...
A key to the development of strategies for manipulating immune responses is the identification of the factors that regulate the generation of memory T cells . Many vaccination strategies rely on multiple injections to boost memory cell numbers , yet the factors that regulate the function and survival of memory T cells ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "activation", "immunity", "to", "infections", "immunology", "vaccination", "and", "immunization", "white", "blood", "cells", "inflammation", "animal", "cells", "t", "cells", "imm...
2014
Dynamic Functional Modulation of CD4+ T Cell Recall Responses Is Dependent on the Inflammatory Environment of the Secondary Stimulus
BICD2 is one of the two mammalian homologues of the Drosophila Bicaudal D , an evolutionarily conserved adaptor between microtubule motors and their cargo that was previously shown to link vesicles and mRNP complexes to the dynein motor . Here , we identified a G2-specific role for BICD2 in the relative positioning of ...
Bidirectional microtubule-based transport is responsible for the positioning of a large variety of cellular organelles , but the molecular mechanisms underlying the recruitment of microtubule-based motors to their cargoes and their activation remain poorly understood . In particular , the molecular players involved in ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/cell", "growth", "and", "division", "cell", "biology", "cell", "biology/cytoskeleton" ]
2010
Bicaudal D2, Dynein, and Kinesin-1 Associate with Nuclear Pore Complexes and Regulate Centrosome and Nuclear Positioning during Mitotic Entry
A diverse subset of pattern recognition receptors ( PRRs ) detects pathogen-associated nucleic acids to initiate crucial innate immune responses in host organisms . Reflecting their importance for host defense , pathogens encode various countermeasures to evade or inhibit these immune effectors . PRRs directly engaged ...
A pathogen’s ability to infect new individuals within and across species is largely driven by its capacity to hijack cellular machinery and overcome the immune system . Pathogens have evolved multiple means to evade and shut down host immunity . Typically , mechanisms of inactivation involve direct interactions between...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods", "and", "Materials" ]
[]
2015
Overlapping Patterns of Rapid Evolution in the Nucleic Acid Sensors cGAS and OAS1 Suggest a Common Mechanism of Pathogen Antagonism and Escape
Yaws and trachoma are targeted for eradication and elimination as public health problems . In trachoma-endemic populations mass administration of azithromycin can simultaneously treat yaws . We conducted a population-based prevalence survey in the five northernmost provinces of Vanuatu , where trachoma and yaws are sus...
Yaws and trachoma are infectious diseases targeted by the World Health Organization ( WHO ) for eradication and elimination as a public health problem , respectively . Both diseases are found in the rural , isolated and underserved communities of the world’s poorest countries . The WHO strategy for trachoma elimination...
[ "Abstract", "Introduction", "Methods", "Household", "Risk", "factors", "Survey", "Statistical", "Analysis", "Ethical", "Considerations", "Results", "Clinical", "Yaws", "Treponemal", "RDT", "Results", "Risk", "Factors", "Associated", "with", "TF", "Discussion" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "chlamydia", "trachomatis", "pathogens", "immunology", "tropical", "diseases", "geographical", "locations", "vanuatu", "microbiology", "health", "care", "treponematoses", "bacterial", "...
2017
Integrated Mapping of Yaws and Trachoma in the Five Northern-Most Provinces of Vanuatu
Snake bite is a major neglected public health issue within poor communities living in the rural areas of several countries throughout the world . An estimated 2 . 5 million people are bitten by snakes each year and the cost and lack of efficacy of current anti-venom therapy , together with the lack of detailed knowledg...
Snake bite is a major neglected public health issue causing an estimated 125 , 000 deaths each year , predominantly within poor communities living in rural areas of countries in South East Asia and Africa . Current treatments for snake bites are costly and have limited effectiveness , thus there is a need to develop no...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biochemistry", "public", "health", "and", "epidemiology", "chemical", "biology/biocatalysis", "infectious", "diseases/neglected", "tropical", "diseases", "biochemistry/protein", "chemistry", "biochemistry/macromolecular", "chemistry", "molecular", "biology/bioinformatics", "comput...
2010
Purification and Functional Characterisation of Rhiminopeptidase A, a Novel Aminopeptidase from the Venom of Bitis gabonica rhinoceros
Nervous systems are information processing networks that evolved by natural selection , whereas very large scale integrated ( VLSI ) computer circuits have evolved by commercially driven technology development . Here we follow historic intuition that all physical information processing systems will share key organizati...
Brains are often compared to computers but , apart from the trivial fact that both process information using a complex physical pattern of connections , it has been unclear whether this is more than just a metaphor . In our work , we rigorously uncover novel quantitative organizational principles that underlie the netw...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "physics/interdisciplinary", "physics", "neuroscience/theoretical", "neuroscience", "computational", "biology/computational", "neuroscience" ]
2010
Efficient Physical Embedding of Topologically Complex Information Processing Networks in Brains and Computer Circuits
RNA silencing , mediated by small RNAs including microRNAs ( miRNAs ) and small interfering RNAs ( siRNAs ) , is a potent antiviral or antibacterial mechanism , besides regulating normal cellular gene expression critical for development and physiology . To gain insights into host small RNA metabolism under infections b...
Small RNA-mediated RNA silencing is a widespread antiviral or antibacterial mechanism in different organisms . Although the host and pathogen factors involved in this mode of host defense and pathogen counter-defense have been extensively investigated , much less is known about how a pathogen alters the small RNA metab...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mechanisms", "of", "resistance", "and", "susceptibility", "plant", "science", "virology", "plant", "pathogens", "plant", "biology", "plant", "pathology", "biology", "microbiology" ]
2011
Viral Infection Induces Expression of Novel Phased MicroRNAs from Conserved Cellular MicroRNA Precursors
Quantitative models of cis-regulatory activity have the potential to improve our mechanistic understanding of transcriptional regulation . However , the few models available today have been based on simplistic assumptions about the sequences being modeled , or heuristic approximations of the underlying regulatory mecha...
The development of complex multicellular organisms requires genes to be expressed at specific stages and in specific tissues . Regulatory DNA sequences , often called cis-regulatory modules , drive the desired gene expression patterns by integrating information about the environment in the form of the activities of tra...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "genetics", "and", "genomics/gene", "expression", "computational", "biology/transcriptional", "regulation", "biophysics/theory", "and", "simulation", "computational", "biology/macromolecular", "sequence", "analysis", "biophysics/transcription", "and", "translation" ]
2010
Thermodynamics-Based Models of Transcriptional Regulation by Enhancers: The Roles of Synergistic Activation, Cooperative Binding and Short-Range Repression
The N-methyl-D-aspartate receptor ( NMDAR ) , a major excitatory ligand-gated ion channel in the central nervous system ( CNS ) , is a principal mediator of synaptic plasticity . Here we report that neuropilin tolloid-like 1 ( Neto1 ) , a complement C1r/C1s , Uegf , Bmp1 ( CUB ) domain-containing transmembrane protein ...
The fundamental unit for information processing in the brain is the synapse , a highly specialized site of communication between the brain's multitude of individual neurons . The strength of the communication at each synapse changes in response to neuronal activity—a process called synaptic plasticity—allowing networks...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neurological", "disorders", "neuroscience", "genetics", "and", "genomics" ]
2009
Neto1 Is a Novel CUB-Domain NMDA Receptor–Interacting Protein Required for Synaptic Plasticity and Learning
The dose response curve is the gold standard for measuring the effect of a drug treatment , but is rarely used in genomic scale transcriptional profiling due to perceived obstacles of cost and analysis . One barrier to examining transcriptional dose responses is that existing methods for microarray data analysis can id...
Transcriptional profiling is arguably the most powerful hypothesis-free method for investigating biological effects of drugs—so why do the experiments typically use outmoded single-dose designs ? Such single-dose experiments will co-mingle effects that can occur with different potency ( e . g . , effects on the known t...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biochemistry/drug", "discovery", "genetics", "and", "genomics/gene", "expression", "biotechnology/chemical", "biology", "of", "the", "cell", "pharmacology/drug", "development", "molecular", "biology/bioinformatics", "computational", "biology/systems", "biology" ]
2009
Transcriptional Profiling of the Dose Response: A More Powerful Approach for Characterizing Drug Activities
The epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell . Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium . Listeria monocyto...
All body surfaces are covered by a single layer of epithelial cells . Epithelial cells form a physical barrier to separate the underlying sterile tissue from the environment . In addition , epithelial cells actively sense bacterial and viral infection . The recognition of pathogenic microorganisms results in cell stimu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/cellular", "microbiology", "and", "pathogenesis", "immunology/innate", "immunity" ]
2010
Potentiation of Epithelial Innate Host Responses by Intercellular Communication
Temporal lobe epilepsy strongly affects hippocampal dentate gyrus granule cells morphology . These cells exhibit seizure-induced anatomical alterations including mossy fiber sprouting , changes in the apical and basal dendritic tree and suffer substantial dendritic spine loss . The effect of some of these changes on th...
Neurogenesis is currently a well known phenomenon in the adult brain , in special in some areas such as the subventricular zone and the dentate gyrus in the hippocampus , in which different endogenous and exogenous factors provoke cell proliferation . In the specific case of the dentate gyrus , granule cells proliferat...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "circuit", "models", "neurobiology", "of", "disease", "and", "regeneration", "medicine", "and", "health", "sciences", "computational", "neuroscience", "neurology", "biology", "and", "life", "sciences", "computational", "biology", "neuroscience" ]
2014
Combined Role of Seizure-Induced Dendritic Morphology Alterations and Spine Loss in Newborn Granule Cells with Mossy Fiber Sprouting on the Hyperexcitability of a Computer Model of the Dentate Gyrus
Plant infection by pathogenic fungi involves the differentiation of appressoria , specialized infection structures , initiated by fungal sensing and responding to plant surface signals . How plant fungal pathogens control infection-related morphogenesis in response to plant-derived signals has been unclear . Here we sh...
Phytopathogenic fungi cause many of the most serious crop diseases . Many fungal pathogens form specialized infection structures , called appressoria in response to plant surface signals . Networks of protein kinase-based signaling pathways regulate a wide variety of key morphological processes including appressorium d...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "anatomy", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "plant", "growth", "and", "development", "enzymes", "distillation", "plant", "embryo", "anatomy", "enzymology", "fungal", "structure", "developmental", "biology", ...
2017
The morphogenesis-related NDR kinase pathway of Colletotrichum orbiculare is required for translating plant surface signals into infection-related morphogenesis and pathogenesis
Biogenesis of ribosomes is an essential cellular process conserved across all eukaryotes and is known to require >170 genes for the assembly , modification , and trafficking of ribosome components through multiple cellular compartments . Despite intensive study , this pathway likely involves many additional genes . Her...
Ribosomes are the extremely complex cellular machines responsible for constructing new proteins . In eukaryotic cells , such as yeast , each ribosome contains more than 80 protein or RNA components . These complex machines must themselves be assembled by an even more complex machinery spanning multiple cellular compart...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/rna-protein", "interactions", "genetics", "and", "genomics/functional", "genomics", "molecular", "biology/nucleolus", "and", "nuclear", "bodies", "molecular", "biology/translation", "mechanisms", "computational", "biology/molecular", "genetics", "genetics", ...
2009
Rational Extension of the Ribosome Biogenesis Pathway Using Network-Guided Genetics
In host and cancer tissues , drug metabolism and susceptibility to drugs vary in a circadian ( 24 h ) manner . In particular , the efficacy of a cell cycle specific ( CCS ) cytotoxic agent is affected by the daily modulation of cell cycle activity in the target tissues . Anti-cancer chronotherapy , in which treatments ...
Chronotherapy of cancers aims at exploiting daily physiological rhythms to improve anti-cancer efficacy and tolerance to drugs by administering treatments at a specific time of the day . Recent clinical trials have shown that chronotherapy can be beneficial in improving quality of life and median life span in patients ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "mathematics/statistics", "cell", "biology/cell", "growth", "and", "division", "computational", "biology/systems", "biology", "oncology/gastrointestinal", "cancers" ]
2010
Tumor Growth Rate Determines the Timing of Optimal Chronomodulated Treatment Schedules
Mosquito-borne diseases are increasingly being recognized as global threats , with increased air travel accelerating their occurrence in travelers and their spread to new locations . Since the early days of aviation , concern over the possible transportation of infected mosquitoes has led to recommendations to disinsec...
Vector-borne diseases such as malaria , dengue , yellow fever , and Zika are global challenges to public health . International policies , such as the International Health Regulations , call for controlling mosquitoes on aircraft to prevent the introduction of mosquito-borne pathogens and invasive mosquito species . Th...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "dengue", "virus", "medicine", "and", "health", "sciences", "parasite", "groups", "pathology", "and", "laboratory", "medicine", "plasmodium", "engineering", "and", "technology", "transportation", "pathogens", "vector-borne", "diseases", "microbiology", "t...
2017
Mosquitoes on a plane: Disinsection will not stop the spread of vector-borne pathogens, a simulation study
The main objective of the study was to determine the degree of sensitization to Anisakis spp . antigens in healthy coastal population of Dalmatia given the high thermally unprocessed fish intake rate present in this area , suggested as a significant risk factor for anisakiasis . We performed a monocenter , cross-sectio...
Anisakiasis is a zoonosis induced by infection with the Anisakis third-stage larvae , contracted through consumption of thermally unprocessed or lightly processed seafood . Its diagnosis is difficult to suspect in countries where the illness was not previously reported , where it is infrequent , or in the cases of subc...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "veterinary", "diseases", "clinical", "immunology", "zoonotic", "diseases", "allergy", "and", "hypersensitivity", "parasitic", "diseases", "veterinary", "science", "helminth", "infection", "foodborne", "diseases" ]
2014
Anti-Anisakis IgE Seroprevalence in the Healthy Croatian Coastal Population and Associated Risk Factors
Robustness of organisms is widely observed although difficult to precisely characterize . Performance can remain nearly constant within some neighborhood of the normal operating regime , leading to homeostasis , but then abruptly break down with pathological consequences beyond this neighborhood . Currently , there is ...
The ability of organisms to survive under a multitude of conditions is readily apparent . This robustness in performance is difficult to precisely characterize and quantify . At a biochemical level , it leads to physiological behavior when the parameters of the system remain within some neighborhood of their normal val...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "hematology/disorders", "of", "red", "cell", "metabolism", "mathematics", "computational", "biology/metabolic", "networks", "biochemistry/theory", "and", "simulation", "computational", "biology/systems", "biology" ]
2009
Quantifying Global Tolerance of Biochemical Systems: Design Implications for Moiety-Transfer Cycles
The cable equation is a proper framework for modeling electrical neural signalling that takes place at a timescale at which the ionic concentrations vary little . However , in neural tissue there are also key dynamic processes that occur at longer timescales . For example , endured periods of intense neural signaling m...
When neurons generate electrical signals they release potassium ions ( K+ ) into the extracellular space . During periods of intense neural activity , the local extracellular K+ may increase drastically . If it becomes too high , it can lead to neural dysfunction . Astrocytes ( a kind of glial cells ) are involved in p...
[ "Abstract", "Introduction", "Model", "Results", "Discussion" ]
[]
2013
Electrodiffusive Model for Astrocytic and Neuronal Ion Concentration Dynamics
Schistosomiasis ( or bilharzia ) , a major parasitic disease , affects more than 260 million people worldwide . In chronic cases of intestinal schistosomiasis caused by trematodes of the Schistosoma genus , hepatic fibrosis develops as a host immune response to the helminth eggs , followed by potentially lethal portal ...
Schistosomiasis ( or bilharzia ) , a major helminth disease , affects more than 260 million people worldwide . While the adult worms survive for years within veins of the gastrointestinal system , symptoms are due to inflammatory reactions to their eggs in several organs . Hepatic fibrosis may develop in chronic cases ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
In Vivo MRI Assessment of Hepatic and Splenic Disease in a Murine Model of Schistosmiasis
Cerebellar Purkinje cells display complex intrinsic dynamics . They fire spontaneously , exhibit bistability , and via mutual network interactions are involved in the generation of high frequency oscillations and travelling waves of activity . To probe the dynamical properties of Purkinje cells we measured their phase ...
By observing how brief current pulses injected at different times between spikes change the phase of spiking of a neuron ( and thus obtaining the so-called phase response curve ) , it should be possible to predict a full spike train in response to more complex stimulation patterns . When we applied this traditional pro...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/neuronal", "signaling", "mechanisms", "neuroscience/theoretical", "neuroscience" ]
2010
A New Approach for Determining Phase Response Curves Reveals that Purkinje Cells Can Act as Perfect Integrators
A significant current challenge in human genetics is the identification of interacting genetic loci mediating complex polygenic disorders . One of the best characterized polygenic diseases is Down syndrome ( DS ) , which results from an extra copy of part or all of chromosome 21 . A short interval near the distal tip o...
A large fraction of human genes may contribute to polygenic disorders , yet few experimental methods for identifying such genes are currently available . For example , with regard to congenital heart defects ( CHD ) caused by extra copies of genes on chromosome 21 in Down syndrome patients , it is not known which genes...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "genome", "expression", "analysis", "cardiovascular", "animal", "models", "model", "organisms", "chromosomal", "disorders", "biology", "down", "syndrome", "congenital", "heart", "disease", "clinical", "genetics", "genetic", "screens", "genetics", "genomics", ...
2011
Over-Expression of DSCAM and COL6A2 Cooperatively Generates Congenital Heart Defects
Infectious disease treatments , both pharmaceutical and vaccine , face three universal challenges: the difficulty of targeting treatments to high-risk ‘superspreader’ populations who drive the great majority of disease spread , behavioral barriers in the host population ( such as poor compliance and risk disinhibition ...
We introduce a proposed intervention against infectious diseases that extends and optimizes the recognized benefit of ‘transmissible immunization’ that occurs with live-attenuated vaccines such as Oral Polio Vaccine ( OPV ) , the vaccine chosen for the worldwide polio eradication campaign . The intervention proposed he...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "immunodeficiency", "viruses", "viruslike", "particles", "population", "modeling", "evolutionary", "modeling", "virology", "synthetic", "biology", "biology", "computational", "biology", "infectious", "disease", "modeling", "microbiology", "molecular", "biology" ]
2011
Autonomous Targeting of Infectious Superspreaders Using Engineered Transmissible Therapies
The Sonic hedgehog ( Shh ) signaling pathway is crucial for pattern formation in early central nervous system development . By systematically analyzing high-throughput in situ hybridization data of E11 . 5 mouse brain , we found that Shh and its receptor Ptch1 define two adjacent mutually exclusive gene expression doma...
Recent large-scale projects of high-throughput in situ hybridization ( ISH ) have generated a wealth of spatiotemporal information on gene expression patterns in the early mouse brain . We have developed a computational approach that combines publicly available high-throughput ISH data with our own experimental data to...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "developmental", "biology", "biology", "and", "life", "sciences", "gene", "regulatory", "networks", "computational", "biology" ]
2014
Reconstruction of the Gene Regulatory Network Involved in the Sonic Hedgehog Pathway with a Potential Role in Early Development of the Mouse Brain
During mismatch repair ( MMR ) MSH proteins bind to mismatches that form as the result of DNA replication errors and recruit MLH factors such as Mlh1-Pms1 to initiate excision and repair steps . Previously , we identified a negative epistatic interaction involving naturally occurring polymorphisms in the MLH1 and PMS1 ...
In nature , bacterial populations with high mutation rates can adapt faster to new environments by acquiring beneficial mutations . However , such populations also accumulate harmful mutations that reduce their fitness . We show that the model eukaryote baker’s yeast can use a similar mutator strategy to adapt to new e...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
A Genetic Incompatibility Accelerates Adaptation in Yeast
Studies on the Bin-Amphiphysin-Rvs ( BAR ) domain have advanced a fundamental understanding of how proteins deform membrane . We previously showed that a BAR domain in tandem with a Pleckstrin Homology ( PH domain ) underlies the assembly of ACAP1 ( Arfgap with Coil-coil , Ankryin repeat , and PH domain I ) into an unu...
Membrane remodeling is needed for a wide range of cellular events . Our current understanding of how proteins bend membrane to achieve such remodeling has come , in large part , from studies on proteins that contain the BAR domain . These studies have elucidated that the BAR domain dimerize to form a crescent-shaped st...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "vesicles", "classical", "mechanics", "protein", "interactions", "membrane", "proteins", "materials", "science", "damage", "mechanics", "protein", "structure", "oligomers", "cellular", "structures", "and", "organelles", "polymer", "chemistry", "proteins", "deformation", "...
2019
ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages
Random Item Generation tasks ( RIG ) are commonly used to assess high cognitive abilities such as inhibition or sustained attention . They also draw upon our approximate sense of complexity . A detrimental effect of aging on pseudo-random productions has been demonstrated for some tasks , but little is as yet known abo...
It has been unclear how this ability evolves over a person’s lifetime and it had not been possible to be assessed with previous classical tools for statistical randomness . To better understand how age impacts behavior , we have assessed more than 3 , 400 people aged 4 to 91 years old . Each participant performed a ser...
[ "Abstract", "Introduction", "Methods", "Results", "and", "discussion" ]
[ "computer", "applications", "education", "decision", "making", "sociology", "social", "sciences", "neuroscience", "age", "groups", "adults", "cognitive", "psychology", "cognition", "computer", "and", "information", "sciences", "behavior", "educational", "attainment", "peo...
2017
Human behavioral complexity peaks at age 25
Transposon mutagenesis , in combination with parallel sequencing , is becoming a powerful tool for en-masse mutant analysis . A probability generating function was used to explain observed miniHimar transposon insertion patterns , and gene essentiality calls were made by transposon insertion frequency analysis ( TIFA )...
Metabolic modeling techniques play a central role in rational design of industrial strains , personalized medicine , and automated network reconstruction . However , due to the large size of models , very few have been comprehensively tested using single gene knockout mutants for every gene in the model . Such a geneti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "biology", "and", "life", "sciences", "microbiology", "computational", "biology" ]
2014
Genome-Scale Metabolic Network Validation of Shewanella oneidensis Using Transposon Insertion Frequency Analysis
Dengue has emerged as a significant public health problem in Sri Lanka . Historically surveillance was passive , with mandatory dengue notifications based on clinical diagnosis with only limited laboratory confirmation . To obtain more accurate data on the disease burden of dengue , we set up a laboratory-based enhance...
The dramatic spread of epidemic dengue underscores the urgent need for better surveillance and control of this disease . The main purpose of surveillance is to provide timely and more accurate information to institute preventive or control measures for epidemic dengue . Dengue has emerged as a major public health probl...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "clinical", "laboratory", "sciences", "geographical", "locations", "tropical", "diseases", "neglected", "tropical", "diseases", "infectious", "disease", "control", "research", "facilities", "research", "and", "analysis", "methods",...
2016
Laboratory-Enhanced Dengue Sentinel Surveillance in Colombo District, Sri Lanka: 2012-2014
The female Aedes aegypti salivary gland plays a pivotal role in bloodmeal acquisition and reproduction , and thereby dengue virus ( DENV ) transmission . It produces numerous immune factors , as well as immune-modulatory , vasodilatory , and anti-coagulant molecules that facilitate blood-feeding . To assess the impact ...
Dengue virus ( DENV ) is transmitted between humans through the bite of infected Aedes aegypti mosquitoes . Since the virus is inoculated in saliva , infection of the mosquito salivary gland is an essential requirement for transmission . In addition , the gland also produces numerous biologically active compounds that ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunity", "virology", "biology", "microbiology" ]
2012
Dengue Virus Infection of the Aedes aegypti Salivary Gland and Chemosensory Apparatus Induces Genes that Modulate Infection and Blood-Feeding Behavior
Enterocytozoon bieneusi is a widespread parasite with high genetic diversity among hosts . Its natural reservoir remains elusive and data on population structure are available only in isolates from primates . Here we describe a population genetic study of 101 E . bieneusi isolates from pigs using sequence analysis of t...
This study explored the genetic characteristics of the ITS and four mini- and microsatellite markers and assessed the population structure and substructures in 101 E . bieneusi isolates from pigs in China . The measures of LD and recombination events supported the occurrence of clonal evolution among the isolates from ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biogeography", "livestock", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "pathology", "and", "laboratory", "medicine", "population", "genetics", "vertebrates", "animals", "mammals", "primates", "population", "biology", "infectio...
2016
Clonal Evolution of Enterocytozoon bieneusi Populations in Swine and Genetic Differentiation in Subpopulations between Isolates from Swine and Humans
Recent advances in sleep neurobiology have allowed development of physiologically based mathematical models of sleep regulation that account for the neuronal dynamics responsible for the regulation of sleep-wake cycles and allow detailed examination of the underlying mechanisms . Neuronal systems in general , and those...
All biological systems are inherently noisy and heterogeneous . Disorder is mostly expected to disturb proper functioning of a system , like it can be the case with noise in a radio signal . However , it has been demonstrated by numerous studies that noise can actually improve signal encoding – the so-called stochastic...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "physics", "computational", "neuroscience", "interdisciplinary", "physics", "biology", "biophysics", "neuroscience" ]
2012
Diversity and Noise Effects in a Model of Homeostatic Regulation of the Sleep-Wake Cycle
Movement interactions and the underlying social structure in groups have relevance across many social-living species . Collective motion of groups could be based on an “egalitarian” decision system , but in practice it is often influenced by underlying social network structures and by individual characteristics . We in...
How does a group of family dogs decide the direction of their collective movements ? Is there a leader , or is decision-making based on an egalitarian system ? Is leadership related to social dominance status ? We collected GPS trajectory data from an owner and her six dogs during several walks . We found that dogs adj...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mathematics", "animal", "behavior", "statistics", "biology", "biostatistics", "zoology" ]
2014
Leadership and Path Characteristics during Walks Are Linked to Dominance Order and Individual Traits in Dogs
Functional characterization of causal variants present on risk haplotypes identified through genome-wide association studies ( GWAS ) is a primary objective of human genetics . In this report , we evaluate the function of a pair of tandem polymorphic dinucleotides , 42 kb downstream of the promoter of TNFAIP3 , ( rs148...
A key objective of human genetics is the identification and characterization of variants responsible for association with complex diseases . A pair of single nucleotide polymorphisms ( rs148314165 , rs200820567 ) 42 kb downstream from the promoter of TNFAIP3 , have been proposed as the variants responsible for associat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
Leprosy is characterized by polar clinical , histologic and immunological presentations . Previous immunologic studies of leprosy polarity were limited by the repertoire of cytokines known at the time . We used a candidate gene approach to measure mRNA levels in skin biopsies from leprosy lesions . mRNA from 24 chemoki...
Leprosy presents with a polarized spectrum , with lepromatous leprosy having high bacillary numbers and TH2 dermal cytokines , versus tuberculoid leprosy showing very few bacilli and TH1 cytokines . The mechanism underlying this polarized presentation is largely unknown . In the following study , we isolated mRNA from ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "bacterial", "diseases", "infectious", "diseases", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immunity", "neglected", "tropical", "diseases", "biology", "and", "life", "sciences", "immunology", "microbiology", "tropical", "diseases", "lepros...
2014
Differential Dermal Expression of CCL17 and CCL18 in Tuberculoid and Lepromatous Leprosy
In oocytes , where centrosomes are absent , the chromosomes direct the assembly of a bipolar spindle . Interactions between chromosomes and microtubules are essential for both spindle formation and chromosome segregation , but the nature and function of these interactions is not clear . We have examined oocytes lacking...
In acentrosomal oocytes , spindle assembly depends on the chromosomes . The nature of the chromosome-microtubule interactions in oocytes that organize spindle bipolarity and orientation of the homologs has been unclear . We have found that several types of functional chromosome-microtubule interactions exist in oocytes...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes
Many algorithms for sequence analysis rely on word matching or word statistics . Often , these approaches can be improved if binary patterns representing match and don’t-care positions are used as a filter , such that only those positions of words are considered that correspond to the match positions of the patterns . ...
We propose a fast algorithm to generate spaced seeds for database searching , read mapping and alignment-free sequence comparison . Spaced seeds—i . e . patterns of match and don’t-care positions—are used by many algorithms for sequence analysis; designing optimal seeds is therefore an active field of research . In seq...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "sequencing", "techniques", "taxonomy", "applied", "mathematics", "split-decomposition", "method", "database", "searching", "simulation", "and", "modeling", "algorithms", "multiple", "alignment", "calculation", "optimization", "phylogenetics", "data", "management", "mathemati...
2016
rasbhari: Optimizing Spaced Seeds for Database Searching, Read Mapping and Alignment-Free Sequence Comparison
Bacillus anthracis produces a binary toxin composed of protective antigen ( PA ) and one of two subunits , lethal factor ( LF ) or edema factor ( EF ) . Most studies have concentrated on induction of toxin-specific antibodies as the correlate of protective immunity , in contrast to which understanding of cellular immun...
Anthrax is of concern with respect to human exposure in endemic regions , concerns about bioterrorism and the considerable global burden of livestock infections . The immunology of this disease remains poorly understood . Vaccination has been based on B . anthracis filtrates or attenuated spore-based vaccines , with mo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "and", "life", "sciences", "medicine", "and", "health", "sciences" ]
2014
Anthrax Lethal Factor as an Immune Target in Humans and Transgenic Mice and the Impact of HLA Polymorphism on CD4+ T Cell Immunity
Zoonotic pathogens such as Ebola and rabies pose a major health risk to humans . One proven approach to minimizing the impact of a pathogen relies on reducing its prevalence within animal reservoir populations using mass vaccination . However , two major challenges remain for vaccination programs that target free-rangi...
Zoonotic pathogens pose a significant health risk to humans . Mass vaccination programs have shown promise for controlling zoonoses in reservoir populations and , in turn , lessening the health burden posed to neighboring human populations . Despite some significant successes , major logistical challenges remain for pr...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "animal", "types", "medicine", "and", "health", "sciences", "zoonotic", "pathogens", "animal", "pathogens", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "tropical", "diseases", "microbiology", "vertebrates", "animals", "mammals", "vaccines", ...
2019
A little goes a long way: Weak vaccine transmission facilitates oral vaccination campaigns against zoonotic pathogens
During development , signaling networks control the formation of multicellular patterns . To what extent quantitative fluctuations in these complex networks may affect multicellular phenotype remains unclear . Here , we describe a computational approach to predict and analyze the phenotypic diversity that is accessible...
The diversity of metazoan life forms that we experience today arose as multicellular systems continually sampled new phenotypes that withstood ever changing selective pressures . This phenotypic diversification is driven by variations in the underlying regulatory network that instructs cells to form multicellular patte...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology/developmental", "evolution", "computational", "biology/signaling", "networks", "computational", "biology/systems", "biology" ]
2009
Predicting Phenotypic Diversity and the Underlying Quantitative Molecular Transitions
Toxoplasma gondii is found worldwide , but distribution of its genotypes as well as clinical expression of human toxoplasmosis varies across the continents . Several studies in Europe , North America and South America argued for a role of genotypes in the clinical expression of human toxoplasmosis . Genetic data concer...
Prevalence of human toxoplasmosis in tropical African countries usually exceeds 50% . Its role as a major opportunistic infection of AIDS patients is regularly described . Due to the lack of investigation , congenital infection is certainly underestimated in Africa . Incidence of Toxoplasma ocular disease is higher in ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/neglected", "tropical", "diseases", "microbiology/parasitology", "infectious", "diseases/protozoal", "infections", "ecology/population", "ecology", "genetics", "and", "genomics/population", "genetics" ]
2010
Additional Haplogroups of Toxoplasma gondii out of Africa: Population Structure and Mouse-Virulence of Strains from Gabon
Respiratory syncytial virus ( RSV ) is the major cause of viral lower respiratory tract illness in children . In contrast to the RSV prototypic strain A2 , clinical isolate RSV 2–20 induces airway mucin expression in mice , a clinically relevant phenotype dependent on the fusion ( F ) protein of the RSV strain . Epider...
Respiratory syncytial virus ( RSV ) is responsible for severe lower respiratory disease in infants and young children . Overabundant airway mucus contributes to airway obstruction in RSV bronchiolitis , and a better understanding of RSV pathogenesis may contribute to needed therapies and vaccines . We reported previous...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "flow", "cytometry", "cell", "physiology", "medicine", "and", "health", "sciences", "respiratory", "infections", "293t", "cells", "biological", "cultures", "egfr", "signaling", "pulmonology", "epithelial", "cells", "animal", "models", "model", "organisms", "research", ...
2016
EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression
Beetle horns are attractive models for studying the evolution of novel traits , as they display diverse shapes , sizes , and numbers among closely related species within the family Scarabaeidae . Horns radiated prolifically and independently in two distant subfamilies of scarabs , the dung beetles ( Scarabaeinae ) , an...
Goliath and Hercules beetles include some of the largest insects known , and the horns they wield are spectacular . These ‘rhinoceros’ beetles form a subfamily within the Scarabaeidae , a clade containing ~35 , 000 primarily hornless species . The other subfamily of horned scarabs , dung beetles , is distantly related ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "rna", "interference", "animals", "dung", "beetles", "animal", "models", "developmental", "biology", "drosophila", "melanogaster", "model", "organisms", "experimental", "organism", "systems", "epigenetics", "drosop...
2018
Rhinoceros beetle horn development reveals deep parallels with dung beetles
Most tissues in multicellular organisms are maintained by continuous cell renewal processes . However , high turnover of many cells implies a large number of error-prone cell divisions . Hierarchical organized tissue structures with stem cell driven cell differentiation provide one way to prevent the accumulation of mu...
We investigate the average stem cell driven dynamics of cell counts in an abstract multi compartment model . Within this framework one can represent different tissue structures , as for example hematopoiesis , the skin or the colonic crypt . Our analysis is based on an individual cell model in which cells can different...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "theoretical", "biology", "disease", "dynamics", "population", "dynamics", "biology", "population", "biology" ]
2011
Dynamics of Mutant Cells in Hierarchical Organized Tissues
Salmonella enterica subspecies can establish persistent , systemic infections in mammals , including human typhoid fever . Persistent S . enterica disease is characterized by an initial acute infection that develops into an asymptomatic chronic infection . During both the acute and persistent stages , the bacteria gene...
Microbes that establish persistent infections present serious problems for world health but are not well understood . The bacteria Salmonella enterica cause asymptomatic chronic infection in humans . Carriers shed the bacteria into the environment , leading to periodic acute typhoid fever epidemics . Antibiotics are ef...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "infectious", "diseases", "microbiology", "mus", "(mouse)", "homo", "(human)", "eubacteria" ]
2007
Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection
Viruses diversify over time within hosts , often undercutting the effectiveness of host defenses and therapeutic interventions . To design successful vaccines and therapeutics , it is critical to better understand viral diversification , including comprehensively characterizing the genetic variants in viral intra-host ...
New sequencing technologies provide unprecedented resolution to study pathogen populations , such as the single stranded RNA viruses HIV , dengue ( DENV ) , and West Nile ( WNV ) , and how they evolve within infected individuals in response to immune , therapeutic , and vaccine pressures . While these new technologies ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genomics", "evolutionary", "biology", "population", "modeling", "population", "genetics", "biology", "computational", "biology", "microbiology", "genetics", "and", "genomics" ]
2012
Highly Sensitive and Specific Detection of Rare Variants in Mixed Viral Populations from Massively Parallel Sequence Data
Evolutionary outcomes depend not only on the selective forces acting upon a species , but also on the genetic background . However , large timescales and uncertain historical selection pressures can make it difficult to discern such important background differences between species . Experimental evolution is one tool t...
Both comparative genomics and experimental evolution are powerful tools that can be used to make inferences about evolutionary processes . Together , these approaches provide the opportunity to observe evolutionary adaptation over millions of years where selective history is largely unknown , and over short timescales ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome", "evolution", "chemical", "compounds", "salts", "fungi", "model", "organisms", "fungal", "evolution", "experimental", "organism", "systems", "saccharomyces", "research", "and", "analysis", "methods", "mycology", "sulfates", "molecular", "evolution", "chemistry", ...
2017
Differential paralog divergence modulates genome evolution across yeast species
Factor H binding protein ( fHbp ) is an important antigen for vaccines against meningococcal serogroup B disease . The protein binds human factor H ( fH ) , which enables the bacteria to resist serum bactericidal activity . Little is known about the vaccine-potential of fHbp for control of meningococcal epidemics in Af...
Epidemics of meningococcal meningitis are common in sub-Saharan Africa . Most are caused by encapsulated serogroup A strains , which rarely cause disease in industrialized countries . A serogroup A polysaccharide protein conjugate vaccine recently was introduced in some countries in sub-Saharan Africa . The antibodies ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "vaccines", "medicine", "vaccination", "clinical", "immunology", "immunity", "vaccine", "development", "biology", "microbiology", "immunology", "bacterial", "pathogens" ]
2011
Meningococcal Factor H Binding Proteins in Epidemic Strains from Africa: Implications for Vaccine Development
Trinucleotide hereditary diseases such as Huntington disease and Friedreich ataxia are cureless diseases associated with inheriting an abnormally large number of DNA trinucleotide repeats in a gene . The genes associated with different diseases are unrelated and harbor a trinucleotide repeat in different functional reg...
Trinucleotide diseases are a broad family of hereditary diseases characterized genetically by an expanded DNA region consisting of a repeated three-letter code . Patients inheriting such an abnormal DNA region experience sudden disease onset at an age that inversely depends on the size of the expanded region , followed...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "homo", "(human)", "genetics", "and", "genomics", "computational", "biology" ]
2007
A Universal Mechanism Ties Genotype to Phenotype in Trinucleotide Diseases
Early malacological literature suggests that the outbreak of schistosomiasis , a parasitic disease transmitted by aquatic snails , in the Senegal River basin occurred due to ecological changes resulting from the construction of the Diama dam . The common treatment , the drug praziquantel , does not protect from the hig...
Schistosomiasis is a chronic parasitic disease that infects millions of people , especially in Africa . Schistosomes are transmitted by direct contact with water sources infested by freshwater snails , which are intermediate hosts for the parasite . The cure in humans is a drug , praziquantel , that kills the mature pa...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biotechnology", "developmental", "biology", "fisheries", "science", "ecology", "agriculture", "ecosystems", "coastal", "ecosystems", "aquaculture", "life", "cycles", "biology", "and", "life", "sciences", "intestinal", "parasites", "freshwater", "ecology", "marine", "biol...
2014
The Prawn Macrobrachium vollenhovenii in the Senegal River Basin: Towards Sustainable Restocking of All-Male Populations for Biological Control of Schistosomiasis
MITA ( also called STING ) is a central adaptor protein in innate immune response to cytosolic DNA . Cellular trafficking of MITA from the ER to perinuclear microsomes after DNA virus infection is critical for MITA activation and onset of innate antiviral response . Here we found that SNX8 is a component of DNA-trigger...
Infection by virus , such as the DNA virus herpes simplex virus 1 , induces the host cells to express proteins that mediate antiviral immune response . The protein called MITA plays an essential role in the process of antiviral immune response . After viral infection , MITA traffics from the endoplasmic reticulum ( ER ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "luciferase", "assay", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "physiology", "cytokines", "hela", "cells", "molecular", "probe", "techniques", "biological", "cultures", "immunology", "biochemical", "analysis", "developmental", "b...
2018
SNX8 modulates innate immune response to DNA virus by mediating trafficking and activation of MITA
The obligate intracellular pathogen Chlamydia trachomatis replicates within a membrane-bound inclusion that acquires host sphingomyelin ( SM ) , a process that is essential for replication as well as inclusion biogenesis . Previous studies demonstrate that SM is acquired by a Brefeldin A ( BFA ) -sensitive vesicular tr...
C . trachomatis is the leading cause of non-congenital blindness in developing countries and is the number one cause of sexually transmitted disease and non-congenital infertility in Western countries . The capacity of Chlamydia infections to lead to infertility and blindness , their association with chronic diseases ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "women's", "health", "biology", "microbiology", "molecular", "cell", "biology" ]
2011
Chlamydia trachomatis Co-opts GBF1 and CERT to Acquire Host Sphingomyelin for Distinct Roles during Intracellular Development
Cytokinins and gibberellins ( GAs ) play antagonistic roles in regulating reproductive meristem activity . Cytokinins have positive effects on meristem activity and maintenance . During inflorescence meristem development , cytokinin biosynthesis is activated via a KNOX-mediated pathway . Increased cytokinin activity le...
Grain number per panicle , a valuable agronomic trait for rice yield improvement , is profoundly affected by reproductive meristem activity . This activity , in turn , is controlled by transcriptional and plant hormone regulators , especially KNOX proteins and cytokinins . However , little is known about the roles of G...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "anatomy", "panicles", "floral", "meristem", "plant", "physiology", "hormones", "cereal", "crops", "plant", "science", "rice", "model", "organisms", "plant", "hormones", "crops", "inflorescences", "plants", "research", "and", "analysis", "methods", "cytokinin...
2016
The QTL GNP1 Encodes GA20ox1, Which Increases Grain Number and Yield by Increasing Cytokinin Activity in Rice Panicle Meristems
Visceral leishmaniasis ( VL ) is the most severe form of leishmaniasis and is potentially fatal if not diagnosed and treated . Accurate and timely diagnosis is considered one of the pillars needed for the reduction in disease-related lethality . Brazil is currently one of the three eco-epidemiological hotspots for this...
Visceral leishmaniasis ( VL ) is a tropical disease distributed worldwide . In the Americas , Brazil reports about 96% of VL cases , which has been highlighted by the increase in lethality in last years . Accurate VL diagnosis is of utmost importance . Despite this , the performance of some commercial tests currently a...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "geographical", "locations", "parasitic", "diseases", "parasitic", "protozoans", "protozoans", "leishmania", "neglected", "tropica...
2019
Performance of serological tests available in Brazil for the diagnosis of human visceral leishmaniasis
Ciliopathies represent a broad class of disorders that affect multiple organ systems . The craniofacial complex is among those most severely affected when primary cilia are not functional . We previously reported that loss of primary cilia on cranial neural crest cells , via a conditional knockout of the intraflagellar...
Primary cilia are ubiquitous organelles that serve to transduce molecular signals within a cell . Loss of functional primary cilia results in a disease class called ciliopathies . Ciliopathies have a broad range of phenotypes; however , severe facial anomalies are commonly associated with this disease class . The facia...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "safranin", "staining", "gene", "regulation", "regulatory", "proteins", "dna-binding", "proteins", "cytoplasmic", "staining", "developmental", "biology", "transcription", "factors", "embryos", "cellular", "structures", "and", "organelles", "research", "and", "analysis", "...
2016
Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline
Cellular and viral S-adenosylmethionine-dependent methyltransferases are involved in many regulated processes such as metabolism , detoxification , signal transduction , chromatin remodeling , nucleic acid processing , and mRNA capping . The Severe Acute Respiratory Syndrome coronavirus nsp16 protein is a S-adenosylmet...
A novel coronavirus emerged in 2003 and was identified as the etiological agent of the deadly disease called Severe Acute Respiratory Syndrome . This coronavirus replicates and transcribes its giant genome using sixteen non-structural proteins ( nsp1-16 ) . Viral RNAs are capped to ensure stability , efficient translat...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biomacromolecule-ligand", "interactions", "biochemistry", "antivirals", "proteins", "virology", "enzymes", "protein", "structure", "emerging", "viral", "diseases", "viral", "enzymes", "biology", "microbiology" ]
2011
Crystal Structure and Functional Analysis of the SARS-Coronavirus RNA Cap 2′-O-Methyltransferase nsp10/nsp16 Complex
Mammalian genomes harbor millions of retrotransposon copies , some of which are transpositionally active . In mouse prospermatogonia , PIWI-interacting small RNAs ( piRNAs ) combat retrotransposon activity to maintain the genomic integrity . The piRNA system destroys retrotransposon-derived RNAs and guides de novo DNA ...
Retrotransposons are a class of transposable elements , of which mobility has mutagenic potential . Therefore , it is important to regulate the expression of retrotransposons for maintaining the genomic integrity . In male germ cells , DNA methylation and the piRNA system are thought to play roles in retrotransposon si...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "sequencing", "techniques", "medicine", "and", "health", "sciences", "reproductive", "system", "spermatocytes", "retrotransposons", "spermatogonia", "germ", "cells", "genetic", "elements", "epigenetics", "dna", "molecular", "biology", "techniques", "rna", "sequencing", "d...
2017
Switching of dominant retrotransposon silencing strategies from posttranscriptional to transcriptional mechanisms during male germ-cell development in mice
DNA methylation is an epigenetic modification involved in regulatory processes such as cell differentiation during development , X-chromosome inactivation , genomic imprinting and susceptibility to complex disease . However , the dynamics of DNA methylation changes between humans and their closest relatives are still p...
Differences in protein coding sequences between humans and their closest relatives are too small to account for their phenotypic differences . It has been hypothesized that these differences may be explained by alterations of gene regulation rather than primary genome sequence . DNA methylation is an important epigenet...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2013
Dynamics of DNA Methylation in Recent Human and Great Ape Evolution
The rate of germline mutation varies widely between species but little is known about the extent of variation in the germline mutation rate between individuals of the same species . Here we demonstrate that an allele that increases the rate of germline mutation can result in a distinctive signature in the genomic regio...
Each time a genome is replicated there is the possibility of error resulting in the incorporation of an incorrect base or bases in the genome sequence . When these errors occur in cells that lead to the production of gametes they can be incorporated into the germline . Such germline mutations are the basis of evolution...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "mutation", "deletion", "mutation", "haplotypes", "nucleic", "acids", "heredity", "germline", "mutation", "gene", "identification", "and", "analysis", "genetics", "mutation", "detection", "biology", "and", "life", "sciences", "dna", "dna", "repair", "a...
2017
Inference of Candidate Germline Mutator Loci in Humans from Genome-Wide Haplotype Data
We performed a Phenome-wide association study ( PheWAS ) utilizing diverse genotypic and phenotypic data existing across multiple populations in the National Health and Nutrition Examination Surveys ( NHANES ) , conducted by the Centers for Disease Control and Prevention ( CDC ) , and accessed by the Epidemiological Ar...
The Epidemiological Architecture for Genes Linked to Environment ( EAGLE ) study performed a Phenome-Wide Association Study ( PheWAS ) to investigate comprehensive associations between a wide range of phenotypes and single-nucleotide polymorphisms using the diverse genotypic and phenotypic data that exists across multi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome-wide", "association", "studies", "statistical", "analysis", "of", "genetic", "association", "genetic", "networks", "genomics", "genome", "analysis", "trait", "locus", "analysis", "genetics", "biology", "and", "life", "sciences", "computational", "biology", "geno...
2014
Detection of Pleiotropy through a Phenome-Wide Association Study (PheWAS) of Epidemiologic Data as Part of the Environmental Architecture for Genes Linked to Environment (EAGLE) Study
Paracoccidioides spp . , a dimorphic pathogenic fungus , is the etiologic agent of paracoccidioidomycosis ( PCM ) . PCM is an endemic disease that affects at least 10 million people in Latin America , causing severe public health problems . The drugs used against pathogenic fungi have various side effects and limited e...
Paracoccidioidomycosis ( PCM ) is a neglected human systemic mycosis caused by Paracoccidioides spp . fungus that invades the host’s lungs and can disseminate to many other organs . Treatment usually involves amphotericin B , sulfadiazine , trimethoprim-sulfamethoxazole , itraconazole , ketoconazole or fluconazole for ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2016
Effects of Argentilactone on the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides spp.
The brain exhibits temporally complex patterns of activity with features similar to those of chaotic systems . Theoretical studies over the last twenty years have described various computational advantages for such regimes in neuronal systems . Nevertheless , it still remains unclear whether chaos requires specific cel...
Cortical circuits exhibit complex temporal patterns of spiking and are exquisitely sensitive to small perturbations in their ongoing activity . These features are all suggestive of an underlying chaotic dynamics . Theoretical works have indicated that a rich dynamical reservoir can endow neuronal circuits with remarkab...
[ "Abstract", "Introduction", "Results", "Discussion", "Models", "Dynamical", "Mean-Field", "Theory", "of", "the", "Single", "Inhibitory", "Population", "Two-population", "networks", "Numerical", "simulations" ]
[]
2015
Asynchronous Rate Chaos in Spiking Neuronal Circuits
Sex determination in mammals is controlled by the presence or absence of the Y-linked gene SRY . In the developing male ( XY ) gonad , sex-determining region of the Y ( SRY ) protein acts to up-regulate expression of the related gene , SOX9 , a transcriptional regulator that in turn initiates a downstream pathway of te...
In mammals , whether an individual develops as a male or female depends on its sex chromosome constitution: those with a Y chromosome become males because of the development of the embryonic gonad into a testis . The Y-linked sex determining gene SRY regulates this process by initiating a pathway of gene and protein ex...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology/germ", "cells", "developmental", "biology/embryology", "genetics", "and", "genomics/animal", "genetics", "developmental", "biology", "genetics", "and", "genomics/functional", "genomics", "genetics", "and", "genomics/disease", "models", "genetics", "a...
2009
Loss of Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) Reveals a Requirement for MAPK Signalling in Mouse Sex Determination
The treatment of Buruli ulcer ( BU ) that is caused by Mycobacterium ulcerans , is currently based on a daily administration of rifampin and streptomycin ( RIF-STR ) . A fully oral intermittent regimen would greatly simplify its treatment on the field . The objective of this study was to assess the bactericidal and ste...
Buruli ulcer , caused by Mycobacterium ulcerans , is the third most frequent disease due to a bacteria of the mycobacteria family , after tuberculosis and leprosy . The disease consists of a cutaneous infection leading to skin ulcerations that can cause severe and debilitating scars . It occurs mostly in Africa where a...
[ "Abstract", "Introduction", "Material", "and", "methods", "Results", "Discussion" ]
[ "antimicrobials", "body", "weight", "medicine", "and", "health", "sciences", "animal", "models", "of", "disease", "drugs", "tropical", "diseases", "microbiology", "animal", "models", "bacterial", "diseases", "model", "organisms", "physiological", "parameters", "pharmace...
2016
Sterilizing Activity of Fully Oral Intermittent Regimens against Mycobacterium Ulcerans Infection in Mice
Riboviruses ( RNA viruses without DNA replication intermediates ) are the most abundant pathogens infecting animals and plants . Only a few riboviral infections can be controlled with antiviral drugs , mainly because of the rapid appearance of resistance mutations . Little reliable information is available concerning i...
Viral disease is a subject of major concern in public health . Diseases produced by riboviruses ( RNA viruses sensu stricto ) represent a special urgency , because these viruses display an exceptional capability to generate resistance mutations against antiviral drugs . Unfortunately , little is known about the rate an...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "model", "organisms", "genetics", "biology", "microbiology", "genetics", "and", "genomics" ]
2012
The Three Faces of Riboviral Spontaneous Mutation: Spectrum, Mode of Genome Replication, and Mutation Rate
β-defensin peptides are a family of antimicrobial peptides present at mucosal surfaces , with the main site of expression under normal conditions in the male reproductive tract . Although they kill microbes in vitro and interact with immune cells , the precise role of these genes in vivo remains uncertain . We show her...
β-defensins are small molecules , considered primarily to be antimicrobials and important in the first defence response to invading organisms . They are predominantly produced at surfaces in contact with the outside environment and these include skin , airway and reproductive tract . We show here that when we delete fr...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Partial Deletion of Chromosome 8 β-defensin Cluster Confers Sperm Dysfunction and Infertility in Male Mice
A community-based longitudinal study was performed in the Eastern Province of Zambia , in which repeated serological samplings were done to determine the incidence of human cysticercosis . Three sampling rounds were carried out at six months intervals . A total of 867 participants presented for all three samplings . Al...
Human neurocysticercosis is an infection of the central nervous system caused by the larval stage of the pork tapeworm ( Taenia solium ) . The infection occurs mainly in developing countries and is associated with poverty , poor sanitation and free-range pig management . It is estimated to be responsible for 30% of cas...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "taeniasis", "medicine", "infectious", "diseases", "public", "health", "and", "epidemiology", "zoonoses", "epidemiology", "infectious", "disease", "epidemiology", "cysticercosis", "neglected", "tropical", "diseases", "parasitic", "diseases" ]
2013
The Incidence of Human Cysticercosis in a Rural Community of Eastern Zambia
The p12 protein is a cleavage product of the Gag precursor of the murine leukemia virus ( MLV ) . Specific mutations in p12 have been described that affect early stages of infection , rendering the virus replication-defective . Such mutants showed normal generation of genomic DNA but no formation of circular forms , wh...
All retroviruses reverse transcribe their RNA genome to a DNA copy in the cytoplasm of the infected cell . To be expressed , the viral genomic DNA has to travel to the cell nucleus and to integrate into the cellular chromosomes . This trafficking is governed by cellular and viral proteins that associate with the viral ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/virion", "structure,", "assembly,", "and", "egress", "virology/effects", "of", "virus", "infection", "on", "host", "gene", "expression", "virology/immunodeficiency", "viruses", "virology/viral", "replication", "and", "gene", "regulation" ]
2010
The Gag Cleavage Product, p12, is a Functional Constituent of the Murine Leukemia Virus Pre-Integration Complex
Following envelope mediated fusion , the HIV-1 core is released into the cytoplasm of the target cell and undergoes a series of trafficking and replicative steps that result in the nuclear import of the viral genome , which ultimately leads to the integration of the proviral DNA into the host cell genome . Previous stu...
Fusion of viral and target cell membranes releases the HIV-1 viral capsid , which houses the viral RNA and proteins necessary for viral reverse transcription and integration , into the cytoplasm of target cells . To complete infection , the viral capsid must ultimately traffic to the nucleus and undergo a process known...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "nuclear", "import", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "hela", "cells", "gene", "regulation", "pathogens", "biological", "cultures", "cell", "processes", "microbiology", "viral", "structure", "retroviruses", "viruses"...
2016
KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection
Protozoan pathogens of the genus Leishmania have evolved unique signaling mechanisms that can sense changes in the host environment and trigger adaptive stage differentiation essential for host cell infection . The signaling mechanisms underlying parasite development remain largely elusive even though Leishmania mitoge...
Leishmaniasis is an important human disease caused by Leishmania parasites . A crucial aspect of Leishmania infectivity is its capacity to sense different environments and adapt for survival inside insect vector and vertebrate host by stage differentiation . This process is triggered by environmental changes encountere...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "mutagenesis", "signal", "transduction", "kinetoplastids", "cell", "biology", "molecular", "biology", "genetics", "biology", "and", "life", "sciences", "molecular", "genetics", "microbiology", "protozoology", "microbial", "genetics", "cell", "signaling", "mapk", "signalin...
2014
Transgenic Analysis of the Leishmania MAP Kinase MPK10 Reveals an Auto-inhibitory Mechanism Crucial for Stage-Regulated Activity and Parasite Viability
Aedes albopictus is a very invasive and aggressive insect vector that causes outbreaks of dengue fever , chikungunya disease , and yellow fever in many countries . Vector ecology and disease epidemiology are strongly affected by environmental changes . Urbanization is a worldwide trend and is one of the most ecological...
Aedes albopictus has expanded its ecological habitat range throughout the world . Although Ae . albopictus was previously considered a rural vector , this species has adapted well to suburban and urban environments , and it has become the most important and sometimes the sole vector of dengue virus transmission in urba...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "medicine", "and", "health", "sciences", "ecology", "epidemiology", "vector", "biology", "dengue", "fever", "neglected", "tropical", "diseases", "biology", "and", "life", "sciences", "vector-borne", "diseases", "viral", "diseases", "tropical", ...
2014
Urbanization Increases Aedes albopictus Larval Habitats and Accelerates Mosquito Development and Survivorship
Plasma insulin oscillations are known to have physiological importance in the regulation of blood glucose . In insulin-secreting β-cells of pancreatic islets , K ( ATP ) channels play a key role in regulating glucose-dependent insulin secretion . In addition , they convey oscillations in cellular metabolism to the memb...
Pulsatile insulin secretion is important for the proper regulation of blood glucose , and disruption of this pulsatility is a hallmark of type II diabetes . An ion channel was discovered more than three decades ago that conveys the metabolic state of insulin-secreting β-cells to the plasma membrane because it is blocke...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "action", "potentials", "body", "fluids", "chemical", "compounds", "diabetic", "endocrinology", "membrane", "potential", "electrophysiology", "carbohydrates", "neuroscience", "organic", "compounds", "glucose", "hormones", "ion", "c...
2017
Upregulation of an inward rectifying K+ channel can rescue slow Ca2+ oscillations in K(ATP) channel deficient pancreatic islets
Vesicle trafficking systems play essential roles in the communication between the organelles of eukaryotic cells and also between cells and their environment . Endocytosis and the late secretory route are mediated by clathrin-coated vesicles , while the COat Protein I and II ( COPI and COPII ) routes stand for the bidi...
Vesicle trafficking systems are fundamental among cellular transport mechanisms; various cargo molecules are transported via different coated vesicles to their specific destinations in every eukaryotic cell . Clathrin-coated vesicles mediate endocytosis and the late secretory route , while the COat Protein I and II ( C...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biochemistry", "proteins", "macromolecular", "assemblies", "biology", "computational", "biology", "molecular", "cell", "biology" ]
2013
Structural Disorder Provides Increased Adaptability for Vesicle Trafficking Pathways
HIV-1 is transmitted primarily across mucosal surfaces and rapidly spreads within the intestinal mucosa during acute infection . The type I interferons ( IFNs ) likely serve as a first line of defense , but the relative expression and antiviral properties of the 12 IFNα subtypes against HIV-1 infection of mucosal tissu...
The therapeutic potential of recombinant IFNα against HIV-1 infection has been explored for 25 years , but its effectiveness was inconsistent . However , these clinical trials administered IFNα2 , which is only one member of a 12-protein family of IFNα subtypes . More recently , IFNα was found to activate ‘restriction ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Interferon-α Subtypes in an Ex Vivo Model of Acute HIV-1 Infection: Expression, Potency and Effector Mechanisms
There is a growing interest in developing novel brain stimulation methods to control disease-related aberrant neural activity and to address basic neuroscience questions . Conventional methods for manipulating brain activity rely on open-loop approaches that usually lead to excessive stimulation and , crucially , do no...
Brain stimulation is being used to ease symptoms in several neurological disorders in cases where pharmacological treatment is not effective ( anymore ) . The most common way for stimulation so far has been to apply a fixed , predetermined stimulus irrespective of the actual state of the brain or the condition of the p...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "action", "potentials", "medicine", "and", "health", "sciences", "neural", "networks", "population", "dynamics", "membrane", "potential", "electrophysiology", "neuroscience", "systems", "science", "mathematics", "algebra", "computational", "neuroscience", "population", "bio...
2016
Recovery of Dynamics and Function in Spiking Neural Networks with Closed-Loop Control
Biological sensory systems react to changes in their surroundings . They are characterized by fast response and slow adaptation to varying environmental cues . Insofar as sensory adaptive systems map environmental changes to changes of their internal degrees of freedom , they can be regarded as computational devices ma...
The ability to process information is a ubiquitous feature of living organisms . Indeed , in order to survive , every living being , from the smallest bacterium to the biggest mammal , has to gather and process information about its surrounding environment . In the same way as our everyday computers need power to funct...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "physics", "thermodynamics", "biophysics", "theory", "biology", "and", "life", "sciences", "physical", "sciences", "biophysics" ]
2014
Thermodynamic Costs of Information Processing in Sensory Adaptation
Yra1 is an essential nuclear factor of the evolutionarily conserved family of hnRNP-like export factors that when overexpressed impairs mRNA export and cell growth . To investigate further the relevance of proper Yra1 stoichiometry in the cell , we overexpressed Yra1 by transforming yeast cells with YRA1 intron-less co...
Yra1 is an essential nuclear RNA-binding protein that plays a role in mRNA export in Saccharomyces cerevisiae . The cellular levels of Yra1 are tightly auto-regulated by splicing of an unusual intron in its pre-mRNA , removal of which causes Yra1 overexpression that results in a dominant-negative growth defect and mRNA...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetic", "networks", "chromosome", "structure", "and", "function", "messenger", "rna", "telomeres", "dna", "replication", "network", "analysis", "genome", "analysis", "protein", "structure", "dna", "molecular", "biology", "techniques", "research", "and", "analysis", ...
2016
Excess of Yra1 RNA-Binding Factor Causes Transcription-Dependent Genome Instability, Replication Impairment and Telomere Shortening
Echinoderms , which are phylogenetically related to vertebrates and produce large numbers of transparent embryos that can be experimentally manipulated , offer many advantages for the analysis of the gene regulatory networks ( GRN ) regulating germ layer formation . During development of the sea urchin embryo , the ect...
Echinoderms ( sea urchins , starfish , etc . ) are marine invertebrates that share a close ancestry with vertebrates . Their embryos offer many advantages for the analysis of transcriptional circuits that control developmental programs . During early development of the common sea urchin Paracentrotus lividus , a signal...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology/morphogenesis", "and", "cell", "biology", "genetics", "and", "genomics/functional", "genomics", "genetics", "and", "genomics/gene", "expression", "developmental", "biology/developmental", "evolution", "developmental", "biology/pattern", "formation", "g...
2010
Ancestral Regulatory Circuits Governing Ectoderm Patterning Downstream of Nodal and BMP2/4 Revealed by Gene Regulatory Network Analysis in an Echinoderm
Access to nutrients is a key factor governing development , reproduction and ultimately fitness . Within social groups , contest-competition can fundamentally affect nutrient access , potentially leading to reproductive asymmetry among individuals . Previously , agent-based models have been combined with the Geometric ...
Getting enough nutrients and at the right balance is among the primary challenges that an animal has to overcome . Animals that live in groups have the added complexity of competition among individuals over foods . We used an evolutionary simulation to explore how the intensity of such competition interacts with the co...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[]
2015
Evolving Nutritional Strategies in the Presence of Competition: A Geometric Agent-Based Model
Homologous recombination ( HR ) is initiated by DNA double-strand breaks ( DSB ) . However , it remains unclear whether single-strand lesions also initiate HR in genomic DNA . Chicken B lymphocytes diversify their Immunoglobulin ( Ig ) V genes through HR ( Ig gene conversion ) and non-templated hypermutation . Both typ...
An important class of chemotherapeutic drugs used in the treatment of cancer induces DNA damage that interferes with DNA replication . The resulting block to replication results in the formation of single-strand gaps in DNA . These gaps can be filled by specialized DNA polymerases , a process associated with the introd...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "immunology", "molecular", "biology/dna", "repair" ]
2009
Genetic Evidence for Single-Strand Lesions Initiating Nbs1-Dependent Homologous Recombination in Diversification of Ig V in Chicken B Lymphocytes
Transient Receptor Potential ( TRP ) channels serve as temperature receptors in a wide variety of animals and must have played crucial roles in thermal adaptation . The TRP vanilloid ( TRPV ) subfamily contains several temperature receptors with different temperature sensitivities . The TRPV3 channel is known to be hig...
Evolution of temperature perception is crucial for adaptation to thermal environments; however , this process is poorly understood . Here we investigated the evolution of the vertebrate TRPV subfamily which contains several mammalian temperature receptors . We identified several novel TRPV genes that have not been foun...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "signal", "transduction", "molecular", "cell", "biology", "molecular", "biology", "genetics", "genetics", "and", "genomics", "biology", "evolutionary", "biology", "comparative", "genomics", "neuroscience", "evolutionary", "processes", "sensory", "perception", "gene", "fun...
2011
Evolution of Vertebrate Transient Receptor Potential Vanilloid 3 Channels: Opposite Temperature Sensitivity between Mammals and Western Clawed Frogs
Affinity maturation and class switching of antibodies requires activation-induced cytidine deaminase ( AID ) -dependent hypermutation of Ig V ( D ) J rearrangements and Ig S regions , respectively , in activated B cells . AID deaminates deoxycytidine bases in Ig genes , converting them into deoxyuridines . In V ( D ) J...
To generate highly specific antibodies in response to an immune challenge , the antibody genes in activated B cells mutate at a very high rate over a period of several days . The enzyme that initiates antibody gene mutation is activation-induced cytidine deaminase ( AID ) , the first protein recognized to directly edit...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "in", "vitro", "immunology", "molecular", "biology" ]
2007
DNA-Dependent Protein Kinase Inhibits AID-Induced Antibody Gene Conversion
The soil-dwelling saprophyte bacterium Burkholderia pseudomallei is the cause of melioidosis , a severe disease of humans and animals in southeast Asia and northern Australia . Despite the detection of B . pseudomallei in various soil and water samples from endemic areas , the environmental habitat of B . pseudomallei ...
Melioidosis is a severe disease affecting humans and animals in the tropics . It is caused by the bacterium Burkholderia pseudomallei , which lives in tropical soil and especially occurs in southeast Asia and northern Australia . Despite the recognition that melioidosis is an emerging infectious disease , little is kno...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/bacterial", "infections", "ecology/environmental", "microbiology", "microbiology/medical", "microbiology", "infectious", "diseases/tropical", "and", "travel-associated", "diseases" ]
2009
Landscape Changes Influence the Occurrence of the Melioidosis Bacterium Burkholderia pseudomallei in Soil in Northern Australia
The JAK/STAT pathway is a key signaling pathway in the regulation of development and immunity in metazoans . In contrast to the multiple combinatorial JAK/STAT pathways in mammals , only one canonical JAK/STAT pathway exists in Drosophila . It is activated by three secreted proteins of the Unpaired family ( Upd ) : Upd...
Innate immunity acts as the primary line of defense to overcome invading organisms . This response starts through sensing of microbe-specific molecules such as lipopolysaccharide and peptidoglycan by host receptors . Metazoans can also recognize signals that are associated with tissue damage and wounding , to then acti...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "animals", "animal", "models", "c-jun", "n-terminal", "kinase", "signaling", "cascade", "bacterial", "diseases", "drosophila", "melanogaste...
2016
Remote Control of Intestinal Stem Cell Activity by Haemocytes in Drosophila
In human cells and in Saccharomyces cerevisiae , BLAP75/Rmi1 acts together with BLM/Sgs1 and TopoIIIα/Top3 to maintain genome stability by limiting crossover ( CO ) formation in favour of NCO events , probably through the dissolution of double Holliday junction intermediates ( dHJ ) . So far , very limited data is avai...
Recombination is a process by which cells can repair DNA damage . Such repair can either be crossovers ( CO ) , in which DNA molecules are submitted to major exchanges , or non-crossover ( NCO ) events . Eukaryotic cells have developed several mechanisms to maintain genome stability during vegetative development by lim...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/recombination", "molecular", "biology/chromosome", "structure", "genetics", "and", "genomics/chromosome", "biology", "cell", "biology/plant", "genetics", "and", "gene", "expression", "molecular", "biology/dna", "repair" ]
2008
The Arabidopsis BLAP75/Rmi1 Homologue Plays Crucial Roles in Meiotic Double-Strand Break Repair
Lepidopterans ( butterflies and moths ) are a rich and diverse order of insects , which , despite their economic impact and unusual biological properties , are relatively underrepresented in terms of genomic resources . The genome of the silkworm Bombyx mori has been fully sequenced , but comparative lepidopteran genom...
Butterflies and moths ( called the Lepidoptera ) are a large and diverse group of insects that has long captured the attention of biologists and laymen . The colorful patterns on the wings of butterflies , in particular , offer an ideal system to investigate which genes and developmental mechanisms contribute to evolut...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "discovery", "genetics", "and", "genomics/comparative", "genomics", "evolutionary", "biology/evolutionary", "and", "comparative", "genetics", "evolutionary", "biology/genomics", "evolutionary", "biology/developmental", "evolution" ]
2009
A Gene-Based Linkage Map for Bicyclus anynana Butterflies Allows for a Comprehensive Analysis of Synteny with the Lepidopteran Reference Genome
It is an assumption of large , population-based datasets that samples are annotated accurately whether they correspond to known relationships or unrelated individuals . These annotations are key for a broad range of genetics applications . While many methods are available to assess relatedness that involve estimates of...
High-density microarrays measuring single nucleotide polymorphisms ( SNPs ) provide information about the genotypes across many loci . SNP genotypes observed for any two individuals can be compared in terms of identity-by-state ( IBS ) , in which two individuals are observed to have 0 , 1 , or 2 alleles in common at a ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "haplotypes", "chromosomal", "inheritance", "heredity", "genetics", "population", "genetics", "biology", "human", "genetics", "genetics", "and", "genomics" ]
2011
Inference of Relationships in Population Data Using Identity-by-Descent and Identity-by-State
The fungal pathogen Candida glabrata has risen from an innocuous commensal to a major human pathogen that causes life-threatening infections with an associated mortality rate of up to 50% . The dramatic rise in the number of immunocompromised individuals from HIV infection , tuberculosis , and as a result of immunosupp...
Candida glabrata is a major human pathogen due to its low susceptibility to conventional antifungal drugs and the dramatic increase in the number of immunocompromised individuals suffering from HIV AIDS , cancer , and diabetes . Iron overload is one of the most common genetically inherited diseases and reports suggest ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/nosocomial", "and", "healthcare-associated", "infections", "microbiology/immunity", "to", "infections", "infectious", "diseases/fungal", "infections", "microbiology/innate", "immunity", "cell", "biology/microbial", "growth", "and", "development", "microbio...
2011
Host Iron Withholding Demands Siderophore Utilization for Candida glabrata to Survive Macrophage Killing
Pulmonary infections are a major global cause of morbidity , exacerbated by an increasing threat from antibiotic-resistant pathogens . In this context , therapeutic interventions aimed at protectively modulating host responses , to enhance defence against infection , take on ever greater significance . Pseudomonas aeru...
Lung infections are a common cause of death worldwide . As the threat of antibiotic-resistance becomes realised , new approaches are needed to treat disease-causing bacteria , such as multidrug-resistant Pseudomonas aeruginosa . Treatments that could enhance the body’s most effective natural defences can overcome antib...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2019
Cathelicidin is a “fire alarm”, generating protective NLRP3-dependent airway epithelial cell inflammatory responses during infection with Pseudomonas aeruginosa
Repurposing existing proteins for new cellular functions is recognized as a main mechanism of evolutionary innovation , but its role in organelle evolution is unclear . Here , we explore the mechanisms that led to the evolution of the centrosome , an ancestral eukaryotic organelle that expanded its functional repertoir...
The centrosome helps cells to divide , and is important for the development of animals . It has its evolutionary origins in the basal body , which was present in the last common ancestor of all eukaryotes . Here , we study how the evolution of novel proteins helped the formation of the centrosome . Coiled-coil proteins...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "sequencing", "techniques", "sequence", "analysis", "biochemistry", "organismal", "evolution", "evolutionary", "modeling", "animal", "evolution", "biology", "and", "life", "sciences", "molecular", "biology", "techniques", "proteomics", "computational", "biology", "evolution...
2014
Coiled-Coil Proteins Facilitated the Functional Expansion of the Centrosome
Cationic amino acid transporters ( CAT ) are important regulators of NOS2 and ARG1 activity because they regulate L-arginine availability . However , their role in the development of Th1/Th2 effector functions following infection has not been investigated . Here we dissect the function of CAT2 by studying two infectiou...
Recent studies conducted with amino transporter Slc7a2-deficient mice ( CAT2 ) demonstrated that NOS2 activity in macrophages is regulated by CAT2 . NOS2 , which synthesizes nitric oxide , regulates numerous important activities , including resistance to infectious organisms , tumor development , and autoimmune disease...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/cellular", "microbiology", "and", "pathogenesis", "infectious", "diseases/neglected", "tropical", "diseases", "gastroenterology", "and", "hepatology/hepatology", "immunology/immune", "response", "infectious", "diseases/helminth", "infections", "infectious", "diseases/pr...
2008
Cationic Amino Acid Transporter-2 Regulates Immunity by Modulating Arginase Activity
FAS-associated factor-1 ( FAF1 ) is a component of the death-inducing signaling complex involved in Fas-mediated apoptosis . It regulates NF-κB activity , ubiquitination , and proteasomal degradation . Here , we found that FAF1 positively regulates the type I interferon pathway . FAF1gt/gt mice , which deficient in FAF...
Type I interferon-mediated antiviral response is critical for controlling virus infections . However , interferon-mediated immune responses need to be tightly regulated to maintain host immune homeostasis . Recently , molecules involved in regulating interferon-mediated innate immune response are the subject of much re...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "innate", "immune", "system", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "immune", "physiology", "cytokines", "antiviral", "immune", "response", "immunology", "microbiology", "light", "microscopy", "viruses", "developmental", "biology", "rna...
2017
FAS-associated factor-1 positively regulates type I interferon response to RNA virus infection by targeting NLRX1
Mucociliary clearance is one of the major lines of defense of the human respiratory system . The mucus layer coating the airways is constantly moved along and out of the lung by the activity of motile cilia , expelling at the same time particles trapped in it . The efficiency of the cilia motion can experimentally be a...
Mucociliary clearance is the first line of defense mechanisms of the human airways . The mucus transporting debris , particles , microorganisms and pollutants is carried away by the coordinated motion of cilia beating at the surface of the airway epithelium . We present here a mathematical and numerical model aiming at...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "velocity", "medicine", "and", "health", "sciences", "body", "fluids", "classical", "mechanics", "fluid", "mechanics", "mechanical", "stress", "cellular", "structures", "and", "organelles", "mucus", "flow", "field", "fluid", "dynamics", "continuum", "mechanics", "flui...
2017
A new index for characterizing micro-bead motion in a flow induced by ciliary beating: Part II, modeling
Trypanosoma cruzi infection is associated with severe T cell unresponsiveness to antigens and mitogens and is characterized by decreased IL-2 synthesis . In addition , the acquisition of the anergic phenotype is correlated with upregulation of “gene related to anergy in lymphocytes” ( GRAIL ) protein in CD4 T cells . W...
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and is endemic in Central and South America , where it affects about 10 million people . In addition , migration has led to the disease being established in non-endemic countries . Infection involves an acute stage that evolves to a chronic stage wher...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "t", "helper", "cells", "flow", "cytometry", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "spleen", "immunology", "parasitic", "diseases", "parasitic", "protozoans", "protozoans", "anergy", "research", "and", ...
2017
GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection
There is a global need for cost-effective and environmentally friendly tools for control of mosquitoes and mosquito-borne diseases . One potential way to achieve this is to combine already available tools to gain synergistic effects to reduce vector mosquito populations . Another possible way to improve mosquito contro...
Traditionally , a key intervention in mosquito control is the use of insecticides against the adult stage . However , various factors limit the long-term use of these control methods , including the development of insecticide resistance , changes in mosquito biting behaviour , and concerns regarding potential negative ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "death", "rates", "larvicides", "invertebrates", "emulsions", "medicine", "and", "health", "sciences", "demography", "colloids", "animals", "reproductive", "physiology", "developmental", "biology", "waxes", "materials", "science", "pest", "control", "population", "biology...
2016
Combining Attractants and Larvicides in Biodegradable Matrices for Sustainable Mosquito Vector Control
Histamine is an important chemical messenger that regulates multiple physiological processes in both vertebrate and invertebrate animals . Even so , how glial cells and neurons recycle histamine remains to be elucidated . Drosophila photoreceptor neurons use histamine as a neurotransmitter , and the released histamine ...
Neurotransmitter transporters that remove neurotransmitters and recycle them after their release have particular importance at visual synapses , which must signal at high frequencies and therefore required rapid clearance of neurotransmitters from the synaptic cleft . In this study , we identified a SLC22 family transp...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
The countries of West Africa are largely portrayed as cholera endemic , although the dynamics of outbreaks in this region of Africa remain largely unclear . To understand the dynamics of cholera in a major portion of West Africa , we analyzed cholera epidemics from 2009 to 2015 from Benin to Mauritania . We conducted a...
We analyzed cholera epidemics from Benin to Mauritania , during 2009 to 2015 , and performed a series of field visits as well as molecular epidemiology analyses of V . cholerae isolates from most recent epidemics throughout West Africa . We found that at least 54% of cases concerned populations living in the three urba...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "guinea", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "vibrio", "tropical", "diseases", "geographical", "locations", "microbiology", "bacterial", "diseases", "vibrio", "cholerae", "benin", "neglected", "tropical", ...
2018
Dynamics of cholera epidemics from Benin to Mauritania
Forecasting the emergence and spread of influenza viruses is an important public health challenge . Timely and accurate estimates of influenza prevalence , particularly of severe cases requiring hospitalization , can improve control measures to reduce transmission and mortality . Here , we extend a previously published...
In the United States , seasonal influenza causes thousands of deaths and hundreds of thousands of hospitalizations . The annual timing and burden of the flu season vary considerably with the severity of the circulating viruses . Epidemic forecasting can inform early and effective countermeasures to limit the human toll...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "influenza", "sociology", "social", "sciences", "social", "media", "mathematics", "forecasting", "statistics", "(mathematics)", "internet", "network", "analysis", "social", "networks", "distribution", "curves", "social", "communica...
2018
Optimal multi-source forecasting of seasonal influenza