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Fusion proteins composed of the histone methyltransferase mixed-lineage leukemia ( MLL ) and a variety of unrelated fusion partners are highly leukemogenic . Despite their prevalence , particularly in pediatric acute leukemia , many molecular details of their transforming mechanism are unknown . Here , we provide mecha...
The expression level of a gene needs to be precisely adjusted to ensure proper function . Adjustments can be imposed at different stages during the overall process of gene expression , including transcription initiation , transcript elongation , and transcript processing . If control of one of these mechanisms fails , ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology", "hematology/acute", "lymphoblastic", "leukemia", "hematology/pediatric", "hematology", "molecular", "biology/transcription", "elongation", "oncology/hematological", "malignancies", "hematology/acute", "myeloid", "leukemia" ]
2009
Misguided Transcriptional Elongation Causes Mixed Lineage Leukemia
The pancreatic islets of Langerhans are multicellular micro-organs integral to maintaining glucose homeostasis through secretion of the hormone insulin . β-cells within the islet exist as a highly coupled electrical network which coordinates electrical activity and insulin release at high glucose , but leads to global ...
As science has successfully broken down the elements of many biological systems , the network dynamics of large-scale cellular interactions has emerged as a new frontier . One way to understand how dynamical elements within large networks behave collectively is via mathematical modeling . Diabetes , which is of increas...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biotechnology", "medicine", "and", "health", "sciences", "engineering", "and", "technology", "membrane", "potential", "electrophysiology", "endocrine", "physiology", "diabetes", "mellitus", "biological", "systems", "engineering", "bioengineering", "computer", "and", "infor...
2014
Phase Transitions in the Multi-cellular Regulatory Behavior of Pancreatic Islet Excitability
Detailed modeling and simulation of biochemical systems is complicated by the problem of combinatorial complexity , an explosion in the number of species and reactions due to myriad protein-protein interactions and post-translational modifications . Rule-based modeling overcomes this problem by representing molecules a...
Rule-based modeling is a modeling paradigm that addresses the problem of combinatorial complexity in biochemical systems . The key idea is to specify only those components of a biological macromolecule that are directly involved in a biochemical transformation . Until recently , this “pattern-based” approach greatly si...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "systems", "biology", "biochemistry", "biochemical", "simulations", "computer", "and", "information", "sciences", "computer", "modeling", "biology", "and", "life", "sciences", "computational", "biology" ]
2014
Exact Hybrid Particle/Population Simulation of Rule-Based Models of Biochemical Systems
Immunizing human volunteers by mosquito bite with radiation-attenuated Plasmodium falciparum sporozoites ( RAS ) results in high-level protection against infection . Only two volunteers have been similarly immunized with P . vivax ( Pv ) RAS , and both were protected . A phase 2 controlled clinical trial was conducted ...
Despite the advances in Plasmodium falciparum ( Pf ) vaccine development , progress in developing P . vivax ( Pv ) vaccines lags far behind . Immunization via mosquito bites with Pf radiation-attenuated sporozoites ( RAS ) has been the gold standard model for induction of sterile protection against malaria infection an...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "immunology", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "animals", "parasitology", "vaccines", "preventive", "medicine", "parasitemia", "bacterial", ...
2016
Protective Efficacy of Plasmodium vivax Radiation-Attenuated Sporozoites in Colombian Volunteers: A Randomized Controlled Trial
Ideal decision-makers should constantly assess all sources of information about opportunities and threats , and be able to redetermine their choices promptly in the face of change . However , perpetual monitoring and reassessment impose inordinate sensing and computational costs , making them impractical for animals an...
Animals should ideally be able to monitor all relevant aspects of their environment constantly and be ever prepared to alter their course of action in the face of unexpected change . However , the impractically high costs of continual monitoring and deliberation mean that a more realistic strategy is required . Here , ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "ecology", "and", "environmental", "sciences", "neurochemistry", "chemical", "compounds", "decision", "making", "predator-prey", "dynamics", "population", "dynamics", "social", "sciences", "neuroscience", "organic", "compounds", "hormones", "cognitive", "psychology", "anima...
2018
Interrupting behaviour: Minimizing decision costs via temporal commitment and low-level interrupts
The role of stochasticity on gene expression is widely discussed . Both potential advantages and disadvantages have been revealed . In some systems , noise in gene expression has been quantified , in among others the lac operon of Escherichia coli . Whether stochastic gene expression in this system is detrimental or be...
Gene expression is a process that is inherently stochastic because of the low number of molecules that are involved . In recent years it has become possible to measure the amount of stochasticity in gene expression , which has inspired a debate about the importance of stochasticity in gene expression . Little attention...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology", "cell", "biology", "eubacteria", "computational", "biology" ]
2007
The Effect of Stochasticity on the Lac Operon: An Evolutionary Perspective
Microsatellite expansions cause a number of dominantly-inherited neurological diseases . Expansions in coding-regions cause protein gain-of-function effects , while non-coding expansions produce toxic RNAs that alter RNA splicing activities of MBNL and CELF proteins . Bi-directional expression of the spinocerebellar at...
We describe several lines of evidence that RNA gain-of-function effects play a significant role in spinocerebellar ataxia type 8 ( SCA8 ) and has broader implications for understanding the CNS effects of other trinucleotide expansion disorders including myotonic dystrophy type 1 , Huntington disease like-2 , and spinoc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/behavioral", "neuroscience", "neuroscience/motor", "systems", "neurological", "disorders/neuroimaging", "neurological", "disorders/movement", "disorders", "neurological", "disorders", "genetics", "and", "genomics/disease", "models", "genetics", "and", "genomics/geneti...
2009
RNA Gain-of-Function in Spinocerebellar Ataxia Type 8
Plant volatiles play important roles in attraction of certain pollinators and in host location by herbivorous insects . Virus infection induces changes in plant volatile emission profiles , and this can make plants more attractive to insect herbivores , such as aphids , that act as viral vectors . However , it is unkno...
Cucumber mosaic virus , an important pathogen of tomato , causes plants to emit volatile chemicals that attract bumblebees . Bumblebees are important tomato pollinators , but do not transmit this virus . We propose that under natural conditions , helping host reproduction by encouraging bee visitation might represent a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "plant", "anatomy", "chemical", "compounds", "brassica", "animals", "organic", "compounds", "plant", "science", "model", "organisms", "crops", "volatile", "organic", "compounds", "plants", "flowering", "plants", "bees", "arabidopsis", "thaliana", "resea...
2016
Virus Infection of Plants Alters Pollinator Preference: A Payback for Susceptible Hosts?
To assess the burden of neurocysticercosis ( NCC ) in California we examined statewide hospital discharge data for 2009 . There were 304 cases hospitalized with NCC identified ( incidence = 0 . 8 per 100 , 000 ) . Cases were mostly Latino ( 84 . 9% ) , slightly more likely to be male than female ( men 57 . 6% , women 4...
Neurocysticercosis ( NCC ) is considered one of the major neglected infections of poverty in the United States , with mortality studies indicating that California bears the highest burden of this disease . Although NCC is a reportable disease in California , studies indicate that this disease goes largely under-reporte...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "mathematical", "computing", "mathematics", "neurological", "disorders", "neurology", "mathematical", "economics" ]
2012
The Impact of Neurocysticercosis in California: A Review of Hospitalized Cases
In China , dengue remains an important public health issue with expanded areas and increased incidence recently . Accurate and timely forecasts of dengue incidence in China are still lacking . We aimed to use the state-of-the-art machine learning algorithms to develop an accurate predictive model of dengue . Weekly den...
Dengue epidemics have posed a great burden expanding of disease , with areas expanding and incidence increasing in China recently . It has remained challenging to develop a robust and accurate forecast model and enhance predictability of dengue incidence . Several state-of-the-art machine learning algorithms , includin...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "machine", "learning", "algorithms", "medicine", "and", "health", "sciences", "china", "atmospheric", "science", "applied", "mathematics", "geographical", "locations", "simulation", "and", "modeling", "algorithms", "regression", "analysis", "mathematics", "forecasting", "...
2017
Developing a dengue forecast model using machine learning: A case study in China
Profiling amino acids and acylcarnitines in whole blood spots is a powerful tool in the laboratory diagnosis of several inborn errors of metabolism . Emerging data suggests that altered blood levels of amino acids and acylcarnitines are also associated with common metabolic diseases in adults . Thus , the identificatio...
Human metabolite levels differ between individuals due to environmental and genetic factors . In the present work , we analyzed whole blood levels of amino acids and acylcarnitines , reflecting disease relevant metabolic pathways , in a cohort of 2 , 107 individuals . We then performed a genome wide association analysi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Integration of Genome-Wide SNP Data and Gene-Expression Profiles Reveals Six Novel Loci and Regulatory Mechanisms for Amino Acids and Acylcarnitines in Whole Blood
Epidemiological studies suggest that allergy risk is preferentially transmitted through mothers . This can be due to genomic imprinting , where the phenotype effect of an allele depends on its parental origin , or due to maternal effects reflecting the maternal genome's influence on the child during prenatal developmen...
Most human diseases are caused by a combination of multiple environmental and genetic influences . The widely used case/control approach aims to identify disease risk genes by comparing the genetic constitution of affected and healthy individuals . Although successful , this approach ignores additional mechanisms influ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Maternal Filaggrin Mutations Increase the Risk of Atopic Dermatitis in Children: An Effect Independent of Mutation Inheritance
The two available drugs for treatment of T . cruzi infection , nifurtimox and benznidazole ( BZ ) , have potential toxic side effects and variable efficacy , contributing to their low rate of use . With scant economic resources available for antiparasitic drug discovery and development , inexpensive , high-throughput a...
The treatment of Trypanosoma cruzi infection ( the cause of human Chagas disease ) remains a significant challenge . Only two drugs , both with substantial toxicity , are available and the efficacy of these dugs is often questioned – in many cases due to the limitations of the methods for assessing efficacy rather than...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/protozoal", "infections", "infectious", "diseases/neglected", "tropical", "diseases", "infectious", "diseases/antimicrobials", "and", "drug", "resistance" ]
2010
In Vitro and In Vivo High-Throughput Assays for the Testing of Anti-Trypanosoma cruzi Compounds
Vibrio cholerae , the causative agent of the cholera disease , is commonly used as a model organism for the study of bacteria with multipartite genomes . Its two chromosomes of different sizes initiate their DNA replication at distinct time points in the cell cycle and terminate in synchrony . In this study , the time-...
Most bacteria encode their genetic information on a single chromosome . The pathogenic bacterium Vibrio cholerae is an exception to this rule and carries two chromosomes of different sizes , each having one origin of replication . A very basic research question is how the replication of the two chromosomes is timed sta...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "bacteriology", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "vibrio", "cell", "processes", "cell", "cycle", "and", "cell", "division", "microbiology", "operons", "vibrio", "cholerae", "rrna", "operons", "dna", ...
2018
Synchronous termination of replication of the two chromosomes is an evolutionary selected feature in Vibrionaceae
Bacterial extracellular polysaccharides are a key constituent of the extracellular matrix material of biofilms . Pseudomonas aeruginosa is a model organism for biofilm studies and produces three extracellular polysaccharides that have been implicated in biofilm development , alginate , Psl and Pel . Significant work ha...
Most bacteria live within biofilm communities , which are a complex population of microorganisms that attach to surfaces and produce copious amounts of extracellular matrix material . Exopolysaccharides are a key feature of the extracellular matrix and are found in many forms , ranging from structurally simple linear h...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "microbiology/microbial", "growth", "and", "development", "microbiology/medical", "microbiology" ]
2011
The Pel Polysaccharide Can Serve a Structural and Protective Role in the Biofilm Matrix of Pseudomonas aeruginosa
There is considerable debate as to the nature of the primary parasite-derived moieties that activate innate pro-inflammatory responses during malaria infection . Microparticles ( MPs ) , which are produced by numerous cell types following vesiculation of the cellular membrane as a consequence of cell death or immune-ac...
Although parasite materials are responsible for the activation of the immune system during malaria infection , exactly how the immune response is initiated during infection is extremely unclear . In this study we demonstrate that sub micron particles ( microparticles ) are produced by malaria infected red blood cells d...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/protozoal", "infections", "pathology/immunology", "immunology/immunity", "to", "infections", "immunology/innate", "immunity" ]
2010
Parasite-Derived Plasma Microparticles Contribute Significantly to Malaria Infection-Induced Inflammation through Potent Macrophage Stimulation
Constitutive heterochromatin is enriched in repetitive sequences and histone H3-methylated-at-lysine 9 . Both components contribute to heterochromatin's ability to silence euchromatic genes . However , heterochromatin also harbors hundreds of expressed genes in organisms such as Drosophila . Recent studies have provide...
The chromosomal domain “heterochromatin” was first defined at the cytological level by its deeply staining appearance compared to more lightly stained domains called “euchromatin . ” Abnormal juxtaposition of these two domains by chromosome rearrangements results in silencing of the nearby euchromatic genes . This effe...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "drosophila", "genetics", "and", "genomics" ]
2008
Molecular Landscape of Modified Histones in Drosophila Heterochromatic Genes and Euchromatin-Heterochromatin Transition Zones
Neuronal information processing is regulated by fast and localized fluctuations of brain states . Brain states reliably switch between distinct spatiotemporal signatures at a network scale even though they are composed of heterogeneous and variable rhythms at a cellular scale . We investigated the mechanisms of this ne...
Brain information processing involves electrophysiological signals at multiple temporal and spatial timescales , from the single neuron level to whole brain areas . A fast and local control of these signals by neurochemicals called neuromodulators is essential in complex tasks such as movement initiation and attentiona...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "action", "potentials", "neural", "networks", "engineering", "and", "technology", "nervous", "system", "electrical", "circuits", "membrane", "potential", "electrophysiology", "neuroscience", "ion", "channels", "co...
2018
Switchable slow cellular conductances determine robustness and tunability of network states
The ability to screen compounds in a high-throughput manner is essential in the process of small molecule drug discovery . Critical to the success of screening strategies is the proper design of the assay , often implying a compromise between ease/speed and a biologically relevant setting . Leishmaniasis is a major neg...
Leishmaniasis , a disease caused by protozoan parasites of the genus Leishmania , is a poverty-related disease threatening 350 million people throughout the world . Drugs currently available to treat this disease are toxic to the patient and drug-resistant parasites are emerging . New therapeutics are therefore needed ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "small", "molecules", "microbiology", "host-pathogen", "interaction", "parasitology", "parastic", "protozoans", "leishmania", "neglected", "tropical", "diseases", "infectious", "diseases", "microbial", "pathogens", "biology", "drug", "discovery", "biochemistry", ...
2011
A Screen against Leishmania Intracellular Amastigotes: Comparison to a Promastigote Screen and Identification of a Host Cell-Specific Hit
The Neurospora crassa mitochondrial tyrosyl-tRNA synthetase ( mtTyrRS; CYT-18 protein ) evolved a new function as a group I intron splicing factor by acquiring the ability to bind group I intron RNAs and stabilize their catalytically active RNA structure . Previous studies showed: ( i ) CYT-18 binds group I introns by ...
The acquisition of new modes of post-transcriptional gene regulation played an important role in the evolution of eukaryotes and was achieved by an increase in the number of RNA-binding proteins with new functions . RNA-binding proteins bind directly to double- or single-stranded RNA and regulate many cellular processe...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "rna-binding", "proteins", "rna", "rna", "structure", "rna", "folding", "protein", "structure", "determination", "rna", "processing", "molecular", "complexes", "proteins", "enzymes", "protein", "structure", "biology", "and", "life", "sciences", "enzymolo...
2014
Evolution of RNA-Protein Interactions: Non-Specific Binding Led to RNA Splicing Activity of Fungal Mitochondrial Tyrosyl-tRNA Synthetases
Neutrophils are known to play a major role in the egg granulomatous lesions caused by Schistosoma japonicum , but the precise mechanism by which eggs recruit or active neutrophil is unknown . Here we report S . japonicum egg specific EF-hand protein-SjE16 . 7 is a potent neutrophil recruiter and initiates the egg assoc...
As a neglected disease , schistosomiasis continues to be a significant cause of parasitic morbidity and mortality worldwide . Schistosoma japonicum is one of the major causative agents of human schistosomiasis . Trapped in the liver or intestinal tissue , S . japonicum eggs are the main cause of pathology following inf...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "parasitology", "biology", "microbiology" ]
2014
Schistosoma japonicum Egg Specific Protein SjE16.7 Recruits Neutrophils and Induces Inflammatory Hepatic Granuloma Initiation
Bolivia has the highest prevalence of Chagas disease ( CD ) in the world ( 6 . 1% ) , with more than 607 , 186 people with Trypanosoma cruzi infection , most of them adults . In Bolivia CD has been declared a national priority . In 2009 , the Chagas National Program ( ChNP ) had neither a protocol nor a clear directive...
Bolivia has the highest prevalence of Chagas disease ( CD ) in the world ( 6 . 1% ) , with more than 607 , 186 people with Trypanosoma cruzi infection . In Bolivia , the management of CD has been declared a national priority . In 2009 , the Chagas National Program ( ChNP ) had neither a protocol nor a clear directive f...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "geographical", "locations", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "health", "care", "organisms", "age", "groups", "adults", "protozoans", "pharmaceutics", "neglected", "tropical", "diseases", ...
2017
A strategy for scaling up access to comprehensive care in adults with Chagas disease in endemic countries: The Bolivian Chagas Platform
Depolarization of presynaptic terminals stimulates calcium influx , which evokes neurotransmitter release and activates phosphorylation-based signalling . Here , we present the first global temporal profile of presynaptic activity-dependent phospho-signalling , which includes two KCl stimulation levels and analysis of ...
Neurobiological processes are altered by linking neuronal activity to regulated changes in protein phosphorylation levels that influence protein function . Although some of the major targets of activity-dependent phospho-signalling have been identified , a large number of substrates remain unknown . Here , we have scre...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "medicine", "and", "health", "sciences", "vesicles", "nervous", "system", "enzymes", "protein", "interaction", "networks", "enzymology", "electrophysiology", "neuroscience", "phosphatases", "synaptic", "vesicles", "synaptosomes", "network", "analysis", "c...
2019
The temporal profile of activity-dependent presynaptic phospho-signalling reveals long-lasting patterns of poststimulus regulation
In recent years different types of structural variants ( SVs ) have been discovered in the human genome and their functional impact has become increasingly clear . Inversions , however , are poorly characterized and more difficult to study , especially those mediated by inverted repeats or segmental duplications . Here...
Inversions have been an evolutionary biology model for almost a century , and recently the discovery of a high amount of structural variation in multiple organisms , including humans , has renewed the interest in them . Since early on , it was shown that they were adaptive and that they were involved in human diseases ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genomics", "genetic", "polymorphism", "genome", "evolution", "genetics", "population", "genetics", "comparative", "genomics", "biology", "human", "genetics", "evolutionary", "biology", "population", "biology", "structural", "genomics" ]
2014
Validation and Genotyping of Multiple Human Polymorphic Inversions Mediated by Inverted Repeats Reveals a High Degree of Recurrence
The vascular wilt fungi Verticillium dahliae and V . albo-atrum infect over 200 plant species , causing billions of dollars in annual crop losses . The characteristic wilt symptoms are a result of colonization and proliferation of the pathogens in the xylem vessels , which undergo fluctuations in osmolarity . To gain i...
Vascular wilts are chronic and very often severe plant diseases that cause billions of dollars in annual crop losses . The characteristic wilt symptom is a result of water blockage caused by the colonization and proliferation of pathogenic microbes in the plant xylem , a water-conducting system . We sequenced genomes o...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "genome", "evolution", "plant", "biology", "microbiology", "genome", "sequencing", "plant", "science", "fungal", "evolution", "microbial", "evolution", "plant", "pathology", "genome", "complexity", "mycology", "microbial", "pathogens", "comparative", "genomics", "biology"...
2011
Comparative Genomics Yields Insights into Niche Adaptation of Plant Vascular Wilt Pathogens
In developing brain neuronal migration , dendrite outgrowth and dendritic spine outgrowth are controlled by Cdc42 , a small GTPase of the Rho family , and its activators . Cdc42 function in promoting actin polymerization is crucial for glutamatergic synapse regulation . Here , we focus on GABAergic synapse-specific act...
GABAergic inhibition regulates distinct stages of brain development; however , cellular mechanisms downstream of GABAA receptors ( GABAARs ) that influence neuronal migration , maturation and synaptogenesis are less clear . ArfGEF9 encodes for RhoGEF with Cdc42 and TC10 GTPase as its substrates . Interestingly , ArhGEF...
[ "Abstract", "Introduction", "Results", "Discussion", "Conclusions", "Materials", "and", "methods" ]
[ "cell", "motility", "medicine", "and", "health", "sciences", "neurogenesis", "nervous", "system", "electrophysiology", "neuroscience", "developmental", "biology", "cellular", "structures", "and", "organelles", "neuronal", "dendrites", "cytoskeleton", "contractile", "protein...
2017
RhoGEF9 splice isoforms influence neuronal maturation and synapse formation downstream of α2 GABAA receptors
Plasmodium yoelii YM asexual blood stage parasites express multiple members of the py235 gene family , part of the super-family of genes including those coding for Plasmodium vivax reticulocyte binding proteins and Plasmodium falciparum RH proteins . We previously identified a Py235 erythrocyte binding protein ( Py235E...
Malaria parasites invade erythrocytes where they develop and multiply before bursting out and invading fresh cells . There are sequential steps to invasion; early in the process , specific parasite proteins bind to molecules on the surface of the erythrocyte . Tight binding forms a junction between parasite and host ce...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "expression", "microbiology/parasitology", "infectious", "diseases/protozoal", "infections", "cell", "biology/cell", "adhesion" ]
2011
Targeted Disruption of py235ebp-1: Invasion of Erythrocytes by Plasmodium yoelii Using an Alternative Py235 Erythrocyte Binding Protein
A crucial step in the development of muscle cells in all metazoan animals is the assembly and anchorage of the sarcomere , the essential repeat unit responsible for muscle contraction . In Caenorhabditis elegans , many of the critical proteins involved in this process have been uncovered through mutational screens focu...
Muscular diseases affect many people worldwide . While we have learned much about the sarcomere , the basic building block of muscle cells , there are still numerous questions that remain to be answered . We must learn more about proteins expressed in muscle and how they interact so that better treatments for myopathie...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "discovery", "cell", "biology/gene", "expression", "cell", "biology", "cell", "biology/cell", "adhesion", "genetics", "and", "genomics", "genetics", "and", "genomics/bioinformatics" ]
2009
An Integrated Strategy to Study Muscle Development and Myofilament Structure in Caenorhabditis elegans
Schistosome parasites cause schistosomiasis , one of the most important infectious diseases worldwide . For decades Praziquantel ( PZQ ) is the only drug widely used for controlling schistosomiasis . The absence of a vaccine and fear of PZQ resistance have motivated the search for alternatives . Studies on protein kina...
Schistosomiasis is an infectious disease caused by schistosome parasites , affecting millions of people worldwide . The pathogenic consequences of schistosomiasis are caused by the eggs inducing severe organ inflammations . Praziquantel is widely used to treat schistosomiasis; however , there is fear of resistance deve...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "genome", "expression", "analysis", "medicine", "and", "health", "sciences", "functional", "genomics", "animal", "genetics", "pathology", "and", "laboratory", "medicine", "tropical", "diseases", "microbiology", "parasitic", "diseases", "parasitology", "parasite", "physiol...
2014
Imatinib Treatment Causes Substantial Transcriptional Changes in Adult Schistosoma mansoni In Vitro Exhibiting Pleiotropic Effects
Following injury , axons of the peripheral nervous system have retained the capacity for regeneration . While it is well established that injury signals require molecular motors for their transport from the injury site to the nucleus , whether kinesin and dynein motors play additional roles in peripheral nerve regenera...
Nerve regeneration requires coordinated responses from multiple cell types after injury . Axons must extend from the neuronal cell body back towards their targets , while surrounding Schwann cells enter a repair cell state in which they promote regeneration . While nerves of the peripheral nervous system can regrow , i...
[ "Abstract", "Introduction", "Results", "Materials", "and", "methods", "Discussion" ]
[ "medicine", "and", "health", "sciences", "microtubules", "nervous", "system", "cell", "processes", "neuroscience", "macroglial", "cells", "motor", "neurons", "nerve", "regeneration", "developmental", "biology", "dyneins", "molecular", "motors", "organism", "development", ...
2019
Dynein promotes sustained axonal growth and Schwann cell remodeling early during peripheral nerve regeneration
While C57BL/6 mice infected in the ear with L . major mount a vigorous Th1 response and resolve their lesions , the Th1 response in C57BL/6 mice infected with L . mexicana is more limited , resulting in chronic , non-healing lesions . The aim of this study was to determine if the limited immune response following infec...
Leishmaniasis , caused by protozoan parasites belonging to the genus , Leishmania , exhibits clinical symptoms ranging from mild cutaneous lesions to more severe cutaneous or visceral disease . Here , we focus on L . major and L . mexicana , two species that lead to self-resolving and chronic cutaneous lesions , respec...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "adaptive", "immunity", "immune", "cells", "monocytes", "immunity", "innate", "immunity", "antigen-presenting", "cells", "parasitology", "immunology", "biology", "microbiology", "host-pathogen", "interaction", "immune", "response" ]
2012
Leishmania mexicana Induces Limited Recruitment and Activation of Monocytes and Monocyte-Derived Dendritic Cells Early during Infection
Allele-specific DNA methylation ( ASM ) is well studied in imprinted domains , but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci , where the ASM is dictated not by parent-of-origin but instead by the local haplotype . We identified loci with strong ASM in human tissues from met...
Allele-specific DNA methylation ( ASM ) is a central mechanism of gene regulation in humans , which can influence inter-individual differences in physical and mental traits and disease susceptibility . ASM is mediated either by parental imprinting , in which the repressed copy ( allele ) of the gene is determined by wh...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "developmental", "biology", "genetics", "biology", "genomics", "evolutionary", "biology", "clinical", "genetics" ]
2013
Comparative Anatomy of Chromosomal Domains with Imprinted and Non-Imprinted Allele-Specific DNA Methylation
The unfolded protein response ( UPR ) in the endoplasmic reticulum ( ER ) and the cytoplasmic heat stress response are two major stress response systems necessary for maintaining proteostasis for cellular health . Failure of either of these systems , such as in sustained UPR activation or in insufficient heat shock res...
Tauopathy including Alzheimer’s disease ( AD ) is characterized by a build-up of tau aggregates in the brain , highly associated with failure of cellular protein homeostasis . Proteostasis can be achieved by protein quality control system to cope with numerous stresses such as proteotoxic stress from misfolded proteins...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "medicine", "and", "health", "sciences", "cellular", "stress", "responses", "neurodegenerative", "diseases", "cell", "processes", "endoplasmic", "reticulum", "brain", "neuroscience", "animal", "models", "protein", "expression", "model", "organisms", "ex...
2017
Bidirectional interplay of HSF1 degradation and UPR activation promotes tau hyperphosphorylation
The tick-borne flavivirus , Kyasanur Forest disease virus ( KFDV ) causes seasonal infections and periodic outbreaks in south-west India . The current vaccine offers poor protection with reported issues of coverage and immunogenicity . Since there are no approved prophylactic therapeutics for KFDV , type I IFN-α/β subt...
Since 1957 Kyasanur Forest disease virus ( KFDV ) has caused seasonal infections and periodic outbreaks in south-west India . It is estimated that nearly 500 people acquire KFDV annually and 3–5% of those infected succumb to the disease . The vaccine strategy is complicated by the lack of coverage , compliance and effi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "japanese", "encephalitis", "virus", "dengue", "virus", "medicine", "and", "health", "sciences", "vesicular", "stomatitis", "virus", "pathology", "and", "laboratory", "medicine", "pathogens", "biological", "cultures", "microbiology", "toxicology", "viruses", "rna", "vir...
2016
Limited Effects of Type I Interferons on Kyasanur Forest Disease Virus in Cell Culture
Recent comprehensive sequence analysis of the maize genome now permits detailed discovery and description of all transposable elements ( TEs ) in this complex nuclear environment . Reiteratively optimized structural and homology criteria were used in the computer-assisted search for retroelements , TEs that transpose b...
Although TEs are a major component of all studied plant genomes , and are the most significant contributors to genome structure and evolution in almost all eukaryotes that have been investigated , their properties and reasons for existence are not well understood in any eukaryotic genome . In order to begin a comprehen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genome", "projects", "genetics", "and", "genomics", "genetics", "and", "genomics/plant", "genomes", "and", "evolution", "genetics", "and", "genomics/genomics" ]
2009
Exceptional Diversity, Non-Random Distribution, and Rapid Evolution of Retroelements in the B73 Maize Genome
Human cytomegalovirus ( HCMV ) infections are life-threating to people with a compromised or immature immune system . Upon adhesion , fusion of the virus envelope with the host cell is initiated . In this step , the viral glycoprotein gB is considered to represent the major fusogen . Here , we present for the first tim...
Human cytomegalovirus ( HCMV ) belongs to the family of β-herpes viruses . HCMV infections are not only life threatening to people with a compromised immune system but also the most common viral cause of congenital defects in newborns . Hence , the development of HCMV vaccines was ranked top priority by the US Institut...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases", "structural", "characterization", "medicine", "and", "health", "sciences", "cell", "biology", "macromolecular", "crystallography", "genetics", "x-ray", "crystallography", "biology", "and", "life", "sciences", "immunology", "congenital", "disorders"...
2014
Structural Basis for the Recognition of Human Cytomegalovirus Glycoprotein B by a Neutralizing Human Antibody
Selective IgA deficiency ( IgAD; serum IgA<0 . 07 g/l ) is the most common form of human primary immune deficiency , affecting approximately 1∶600 individuals in populations of Northern European ancestry . The polygenic nature of IgAD is underscored by the recent identification of several new risk genes in a genome-wid...
The human leukocyte antigen ( HLA ) locus is robustly associated with many immune-mediated conditions . However , identification of the genetic variants contributing to the disease pathophysiology has been greatly hampered by the extensive chromosomal conservation within this genomic region . To better understand the a...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "immunity", "genetics", "population", "genetics", "biology", "immunology", "genetics", "and", "genomics" ]
2012
High-Density SNP Mapping of the HLA Region Identifies Multiple Independent Susceptibility Loci Associated with Selective IgA Deficiency
Nucleoporins build the nuclear pore complex ( NPC ) , which , as sole gate for nuclear-cytoplasmic exchange , is of outmost importance for normal cell function . Defects in the process of nucleocytoplasmic transport or in its machinery have been frequently described in human diseases , such as cancer and neurodegenerat...
Fetal movement is a prerequisite for normal fetal development and growth . Fetal akinesia deformation sequence ( FADS ) is the result of decreased fetal movement coinciding with congenital malformations related to impaired fetal movement . FADS may be caused by heterogenous defects at any point along the motor system p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "fish", "neuromuscular", "junctions", "medicine", "and", "health", "sciences", "hela", "cells", "nervous", "system", "biological", "cultures", "vertebrates", "electrophysiology", "neuroscience", "animals", "biological", "locomotion", "animal", "models", "osteichthyes", "d...
2018
Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence
River blindness ( onchocerciasis ) causes severe itching , skin lesions , and vision impairment including blindness . More than 99% of all current cases are found in sub-Saharan Africa . Fortunately , vector control and community-directed treatment with ivermectin have significantly reduced morbidity . Studies in Mali ...
River blindness ( onchocerciasis ) is transmitted by blackflies and causes severe itching , skin lesions , and vision impairment including blindness . More than 99% of all current cases are found in sub-Saharan Africa where the disease has historically hindered socioeconomic development in endemic areas . The treatment...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Control, Elimination, and Eradication of River Blindness: Scenarios, Timelines, and Ivermectin Treatment Needs in Africa
We introduce a computational model to simulate chromatin structure and dynamics . Starting from one-dimensional genomics and epigenomics data that are available for hundreds of cell types , this model enables de novo prediction of chromatin structures at five-kilo-base resolution . Simulated chromatin structures recapi...
Three-dimensional genome organization is expected to play crucial roles in regulating gene expression and establishing cell fate , and has inspired the development of numerous innovative experimental techniques for its characterization . Though significant progress has been made , it remains challenging to construct ch...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "chromosome", "structure", "and", "function", "histone", "modification", "chromosome", "mapping", "mathematics", "materials", "science", "algebra", "epigenetics", "molecular", "biology", "techniques", "macromolecules", "structural", "genomics", "chromatin", "research", "and...
2019
Predicting three-dimensional genome organization with chromatin states
Gaining insights into genetic predisposition to age-related diseases and lifespan is a challenging task complicated by the elusive role of evolution in these phenotypes . To gain more insights , we combined methods of genome-wide and candidate-gene studies . Genome-wide scan in the Atherosclerosis Risk in Communities (...
Biomedical research and medical care are traditionally focused on individual health conditions in order to postpone , ameliorate , or prevent the accumulation of morbidities in late life . An attractive idea is to find factors , which could reduce burden of not just one disease but a major subset of them to efficiently...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "neurodegenerative", "diseases", "biomarkers", "diabetes", "mellitus", "endocrine", "disorders", "coronary", "heart", "disease", "mathematics", "statistics", "(mathematics)", "alzheimer", "disease", "cardiology", "research", "and", ...
2016
Pleiotropic Associations of Allelic Variants in a 2q22 Region with Risks of Major Human Diseases and Mortality
Transcription factor ( TF ) proteins recognize a small number of DNA sequences with high specificity and control the expression of neighbouring genes . The evolution of TF binding preference has been the subject of a number of recent studies , in which generalized binding profiles have been introduced and used to impro...
Transcription factors are primary regulators of gene expression . They usually recognize short DNA sequences in gene promoters and subsequently alter their transcription rate . It is known that structurally related transcription factors often recognize similar DNA-binding patterns ( or motifs ) . Comparison of these mo...
[ "Abstract", "Introduction", "Results", "Discussion", "Supporting", "Information" ]
[ "eukaryotes", "computational", "biology" ]
2007
DNA Familial Binding Profiles Made Easy: Comparison of Various Motif Alignment and Clustering Strategies
Invertebrate stages of Leishmania are capable of genetic exchange during their extracellular growth and development in the sand fly vector . Here we explore two variables: the ability of diverse L . major strains from across its natural range to undergo mating in pairwise tests; and the timing of the appearance of hybr...
Leishmania are pathogenic protozoa characterized by substantial diversity in the sand fly species that can transmit them , in the mammalian species that can serve as their reservoir hosts , and in the disease forms and severity of the clinical outcomes they can produce in humans . The possibility that this diversity ha...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "biology" ]
2013
The Mating Competence of Geographically Diverse Leishmania major Strains in Their Natural and Unnatural Sand Fly Vectors
Stem cells reside in a particular microenvironment known as a niche . The interaction between extrinsic cues originating from the niche and intrinsic factors in stem cells determines their identity and activity . Maintenance of stem cell identity and stem cell self-renewal are known to be controlled by chromatin factor...
Tissue maintenance and repair rely on adult stem cells , which can divide to generate new stem cells as well as cells committed for becoming specific cell types . Stem cell activity needs to be tightly controlled because insufficient or unlimited stem cell division may lead to tissue degeneration or tumorigenesis . Thi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "reproductive", "system", "rna", "interference", "animals", "cell", "differentiation", "germ", "cells", "animal", "models", "developmental", "biology", "drosophila", "melanogaster", "model", "organisms", "stem", ...
2017
Enhancer of polycomb coordinates multiple signaling pathways to promote both cyst and germline stem cell differentiation in the Drosophila adult testis
Chromosomal structural change triggers carcinogenesis and the formation of other genetic diseases . The breakpoint junctions of these rearrangements often contain small overlapping sequences called “microhomology , ” yet the genetic pathway ( s ) responsible have yet to be defined . We report a simple genetic system to...
Cancer results from an accumulation of mutations that transform a normal cell into one that proliferates uncontrollably . DNA double-strand breaks ( DSBs ) can lead to genetic mutations and chromosome rearrangements , underscoring the importance of functional DNA DSB repair pathways in the maintenance of chromosome int...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetics", "biology", "molecular", "cell", "biology", "genetics", "and", "genomics" ]
2012
Microhomology Directs Diverse DNA Break Repair Pathways and Chromosomal Translocations
Simulation of biomolecular networks is now indispensable for studying biological systems , from small reaction networks to large ensembles of cells . Here we present a novel approach for stochastic simulation of networks embedded in the dynamic environment of the cell and its surroundings . We thus sample trajectories ...
Simulation algorithms have become indispensable tools in modern quantitative biology , providing deep insight into many biochemical systems , including gene regulatory networks . However , current stochastic simulation approaches handle the effects of fluctuating extracellular signals and upstream processes poorly , ei...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "signaling", "networks", "cell", "differentiation", "circadian", "oscillators", "simulation", "and", "modeling", "developmental", "biology", "mathematics", "network", "analysis", "chronobiology", "research", "and", "analysis", "methods", "computer", "and", "information", ...
2016
Stochastic Simulation of Biomolecular Networks in Dynamic Environments
The Caenorhabditis elegans class A and B synthetic multivulva ( synMuv ) genes redundantly antagonize an EGF/Ras pathway to prevent ectopic vulval induction . We identify a class A synMuv mutation in the promoter of the lin-3 EGF gene , establishing that lin-3 is the key biological target of the class A synMuv genes in...
Extracellular signals that drive cells to divide must be carefully restricted so that only the correct cells receive those signals . Failure to properly control the expression of signaling molecules can lead to aberrant development and cancer . Studies of vulval development in the nematode Caenorhabditis elegans have h...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "animal", "genetics", "gene", "regulation", "cell", "differentiation", "animal", "models", "developmental", "biology", "caenorhabditis", "elegans", "model", "organisms", "organism", "development", "molecular", "development", "molecular", "genetics", "pattern", "formation", ...
2011
The Caenorhabditis elegans Synthetic Multivulva Genes Prevent Ras Pathway Activation by Tightly Repressing Global Ectopic Expression of lin-3 EGF
In most sexually reproducing organisms , the fundamental process of meiosis is implemented concurrently with two differentiation programs that occur at different rates and generate distinct cell types , sperm and oocytes . However , little is known about how the meiotic program is influenced by such contrasting develop...
Sperm and oocytes contribute equal but unique complements of DNA to each new life . Both types of cells arise from meiosis , a multi-step program during which chromosomes replicate , pair and recombine , then divide to generate haploid gametes . Simultaneously , each cell type also differentiates via distinct developme...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology/germ", "cells", "cell", "biology/nuclear", "structure", "and", "function", "cell", "biology/cell", "signaling", "cell", "biology/cell", "growth", "and", "division", "genetics", "and", "genomics/nuclear", "structure", "and", "function", "cell", ...
2009
Spermatogenesis-Specific Features of the Meiotic Program in Caenorhabditis elegans
In this study we compared the utility of two molecular biology techniques , cloning of the mitochondrial 12S ribosomal RNA gene and hydrolysis probe-based qPCR , to identify blood meal sources of sylvatic Chagas disease insect vectors collected with live-bait mouse traps ( also known as Noireau traps ) . Fourteen T . g...
The World Health Organization ( WHO ) estimates that 7 to 8 million people are currently infected with Trypanosoma cruzi , the parasite that causes Chagas disease . The WHO recommends insect vector control as the primary prevention method; and insecticide spraying is the most commonly used intervention technique . Sylv...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "medicine", "and", "health", "sciences", "chagas", "disease", "neglected", "tropical", "diseases", "tropical", "diseases", "protozoan", "infections", "parasitic", "diseases" ]
2014
Sources of Blood Meals of Sylvatic Triatoma guasayana near Zurima, Bolivia, Assayed with qPCR and 12S Cloning
Diverse sex-chromosome systems are found in vertebrates , particularly in teleost fishes , where different systems can be found in closely related species . Several mechanisms have been proposed for the rapid turnover of sex chromosomes , including the transposition of an existing sex-determination gene , the appearanc...
Sex chromosomes have independently evolved many times in animals , plants , and fungi . Although some sex chromosomes have been maintained across different species for over a hundred million years , there are other systems in which sex chromosomes appear to turn over within a few million years . Because sex determinati...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/chromosome", "biology", "evolutionary", "biology/evolutionary", "and", "comparative", "genetics" ]
2009
Turnover of Sex Chromosomes in the Stickleback Fishes (Gasterosteidae)
Efficient cognitive decisions should be adjustable to incoming novel information . However , most current models of decision making have so far neglected any potential interaction between intentional and stimulus-driven decisions . We report here behavioral results and a new model on the interaction between a perceptua...
The topic of our study is the interaction between intentional and externally-driven actions . The contemporary literature on motor control in primates clearly distinguishes two neural mechanisms for these two types of actions . We believe that this distinction is artefactual and comes in part from the fact that intenti...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "psychology", "cognitive", "psychology", "social", "and", "behavioral", "sciences", "mental", "health", "computational", "neuroscience", "biology", "neuroscience" ]
2013
Dual Process for Intentional and Reactive Decisions
Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer , cell fusion , and iPS . Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division . We show here that , after nuclear transfer to amphibian oocytes , mi...
Cells are dividing very actively at a time in development when new gene expression and new cell lineages arise . At mitosis , most transcription factors are temporarily displaced from chromosomes . We show that , after transplantation to oocytes , somatic cell nuclei that have been synchronized in mitosis can be reprog...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "and", "life", "sciences" ]
2014
Mitosis Gives a Brief Window of Opportunity for a Change in Gene Transcription
Adaptive collective systems are common in biology and beyond . Typically , such systems require a task allocation algorithm: a mechanism or rule-set by which individuals select particular roles . Here we study the performance of such task allocation mechanisms measured in terms of the time for individuals to allocate t...
Many complex systems have to allocate their units to different functions: cells in an embryo develop into different tissues , servers in a computer cluster perform different calculations , and insect workers choose particular tasks , such as brood care or foraging . Here we demonstrate that this process does not automa...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "honey", "bees", "applied", "mathematics", "sociology", "social", "sciences", "animals", "simulation", "and", "modeling", "social", "systems", "algorithms", "systems", "science", "mathematics", "animal", "behavior", "probability", "distribution", "zoology...
2017
Costs of task allocation with local feedback: Effects of colony size and extra workers in social insects and other multi-agent systems
Mutations of the Wnt5a gene , encoding a ligand of the non-canonical Wnt pathway , and the Ror2 gene , encoding its receptor , have been found in patients with cardiac outflow tract defects . We found that Wnt5a is expressed in the second heart field ( SHF ) , a population of cardiac progenitor cells destined to popula...
We have demonstrated a novel interaction between the Tbx1 gene , the mutation of which causes DiGeorge syndrome , and Wnt5a , another human disease gene , which is important for oriented cell migration and cell polarity . We found that , in mice , reduced dosage of each of the two genes enhances the phenotype caused by...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "developmental", "biology", "model", "organisms", "genetics", "biology", "molecular", "cell", "biology", "genetics", "and", "genomics" ]
2012
Transcriptional Control in Cardiac Progenitors: Tbx1 Interacts with the BAF Chromatin Remodeling Complex and Regulates Wnt5a
Enteropathogenic and enterohemorrhagic E . coli ( EPEC and EHEC ) are closely related extracellular pathogens that reorganize host cell actin into “pedestals” beneath the tightly adherent bacteria . This pedestal-forming activity is both a critical step in pathogenesis , and it makes EPEC and EHEC useful models for stu...
Microbial pathogens that rearrange the host actin cytoskeleton have made valuable contributions to our understanding of cell signaling and movement . The assembly and organization of the actin cytoskeleton is driven by proteins called nucleators , which can be manipulated by bacteria including enteropathogenic Escheric...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "hela", "cells", "gene", "regulation", "pathogens", "biological", "cultures", "cell", "processes", "condensed", "matter", "physics", "cell", "cultures", "research",...
2018
Enteropathogenic E. coli relies on collaboration between the formin mDia1 and the Arp2/3 complex for actin pedestal biogenesis and maintenance
MicroRNAs ( miRNAs ) and trans-acting siRNAs ( ta-siRNAs ) are essential to the establishment of adaxial–abaxial ( dorsoventral ) leaf polarity . Tas3-derived ta-siRNAs define the adaxial side of the leaf by restricting the expression domain of miRNA miR166 , which in turn demarcates the abaxial side of leaves by restr...
Small RNAs regulate many key developmental processes . Consistent with a prominent role in development , miRNAs exhibit complex and distinctive expression patterns . In this study , we identify regulatory mechanisms that allow for the precise spatial accumulation of developmentally important small RNAs in plants . Plan...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "plant", "biology/plant", "genetics", "and", "gene", "expression", "developmental", "biology/plant", "growth", "and", "development", "developmental", "biology/pattern", "formation" ]
2009
Regulation of Small RNA Accumulation in the Maize Shoot Apex
In systems and computational biology , much effort is devoted to functional identification of systems and networks at the molecular-or cellular scale . However , similarly important networks exist at anatomical scales such as the tendon network of human fingers: the complex array of collagen fibers that transmits and d...
In science and medicine alike , one of the critical steps to understand the working of organisms is to identify how a given individual is similar or different from others . Only then can the specific features of an individual be distinguished from the general properties of that species . However , doing enough input-ou...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biotechnology", "bioengineering", "computer", "science", "computer", "modeling", "biology", "computational", "biology", "engineering" ]
2012
Functional Inference of Complex Anatomical Tendinous Networks at a Macroscopic Scale via Sparse Experimentation
The multifactorial nature of disease motivates the use of systems-level analyses to understand their pathology . We used a systems biology approach to study tau aggregation , one of the hallmark features of Alzheimer's disease . A mathematical model was constructed to capture the current state of knowledge concerning t...
Neurodegenerative disorders , particularly the tauopathy Alzheimer's disease , affect millions of people and cost billions of dollars a year in healthcare costs . Although effective treatments to delay or reverse cognitive decline are still unavailable , several approaches to address this medical need are being pursued...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/systems", "biology", "neurological", "disorders" ]
2010
Vulnerabilities in the Tau Network and the Role of Ultrasensitive Points in Tau Pathophysiology
Leishmania donovani , a protozoan parasite is the major causative agent of visceral leishmaniasis . Increased toxicity and resistance to the existing repertoire of drugs has been reported . Hence , an urgent need exists for identifying newer drugs and drug targets . Previous reports have shown sirtuins ( Silent Informa...
Sirtuins are present in most organisms , including plants , bacteria , and animals . They play a vital role in promoting an organism’s health and survival . These proteins are involved in the regulation of several functions in eukaryotic cells , including transcriptional repression , recombination , cell cycle , cellul...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "parasitic", "cell", "cycles", "split-decomposition", "method", "microbiology", "parasitic", "diseases", "protozoan", "life", "cycles", "parasitic", "protozoans", "parasitology", "multiple", "alignment", "calculation", "developmental...
2017
The mitochondrial SIR2 related protein 2 (SIR2RP2) impacts Leishmania donovani growth and infectivity
Cotrimoxazole prevents opportunistic infections including falciparum malaria in HIV-infected individuals but there are concerns of cross-resistance to other antifolate drugs such as sulphadoxine-pyrimethamine ( SP ) . In this study , we investigated the prevalence of antifolate-resistance mutations in Plasmodium falcip...
Cotrimoxazole , an antifolate , is a fixed-dose trimethoprim-sulfamethoxazole used to prevent opportunistic infections including malaria in HIV-infected individuals . There are concerns that widespread use of cotrimoxazole for prophylaxis may result in selection of P . falciparum parasites with cross-resistance to othe...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "parasite", "groups", "plasmodium", "tropical", "diseases", "parasitic", "diseases", "parasitic", "protozoans", "genetic", "mapping", "parasitology", "preventive", "medicine", "mutation", "apicomplexa", "protozoans", "public", "and...
2019
The prevalence and antifolate drug resistance profiles of Plasmodium falciparum in study participants randomized to discontinue or continue cotrimoxazole prophylaxis
Bovine tuberculosis ( bTB ) caused by Mycobacterium bovis is an important re-emerging disease affecting livestock , wildlife and humans . Epidemiological studies are crucial to identifying the source of bTB infection , and its transmission dynamics and host preference , and thus to the implementation of effective strat...
Bovine tuberculosis is a widespread infectious disease affecting both domestic and wild animals , as well as humans . In addition to being of public health concern , the disease , caused mainly by Mycobacterium bovis , has a significant economic impact on the farming industry due to the costs of eradication efforts . I...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biogeography", "livestock", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "ruminants", "population", "genetics", "vertebrates", "animals", "mammals", "farms", "population", "biology", "bacteria", "veterinary", "science", "genetic...
2019
Genotype diversity and distribution of Mycobacterium bovis from livestock in a small, high-risk area in northeastern Sicily, Italy
Rabies virus ( RABV ) is a neurotropic virus that depends on long distance axonal transport in order to reach the central nervous system ( CNS ) . The strategy RABV uses to hijack the cellular transport machinery is still not clear . It is thought that RABV interacts with membrane receptors in order to internalize and ...
Rabies virus ( RABV ) is a neurotropic virus that depends on long distance axonal transport in order to reach the central nervous system ( CNS ) . The strategy RABV uses to hijack the cellular transport machinery is unknown . Here we use live cell imaging to track RABV entry at nerve terminals and study its retrograde ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience", "cell", "biology", "biology", "and", "life", "sciences", "molecular", "biology" ]
2014
Rabies Virus Hijacks and Accelerates the p75NTR Retrograde Axonal Transport Machinery
Quantification of gene expression levels at the single cell level has revealed that gene expression can vary substantially even across a population of homogeneous cells . However , it is currently unclear what genomic features control variation in gene expression levels , and whether common genetic variants may impact ...
Common genetic variation can alter the level of average gene expression in human tissues , and through changes in gene expression have downstream consequences on cell function , human development , and human disease . However , human tissues are composed of many cells , each with its own level of gene expression . With...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[]
2019
Discovery and characterization of variance QTLs in human induced pluripotent stem cells
Stimulus-specific adaptation ( SSA ) in single neurons of the auditory cortex was suggested to be a potential neural correlate of the mismatch negativity ( MMN ) , a widely studied component of the auditory event-related potentials ( ERP ) that is elicited by changes in the auditory environment . However , several aspe...
Sensory systems automatically detect salient events in a monotonous ambient background . In humans , this change detection process is indexed by the mismatch negativity ( MMN ) , a mid-late component of the auditory-evoked potentials that has become a central tool in cognitive and clinical neuroscience over the last 40...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "auditory", "cortex", "medicine", "and", "health", "sciences", "action", "potentials", "engineering", "and", "technology", "signal", "processing", "topographic", "maps", "membrane", "potential", "brain", "brain", "electrophysiology", "electrophysiology", "neuroscience", "...
2016
Topographic Distribution of Stimulus-Specific Adaptation across Auditory Cortical Fields in the Anesthetized Rat
Collective motion phenomena in large groups of social organisms have long fascinated the observer , especially in cases , such as bird flocks or fish schools , where large-scale highly coordinated actions emerge in the absence of obvious leaders . However , the mechanisms involved in this self-organized behavior are st...
Swarms of insects , schools of fish and flocks of birds display an impressive variety of collective patterns that emerge from local interactions among group members . These puzzling phenomena raise a variety of questions about the behavioral rules that govern the coordination of individuals' motions and the emergence o...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "statistical", "mechanics", "neuroscience", "animal", "behavior", "animal", "management", "phase", "transformation", "biology", "agriculture", "behavioral", "ecology", "physics", "systems", "biology", "computer", "science", "condensed-matter", "physics", "computer", "modeli...
2012
Deciphering Interactions in Moving Animal Groups
Enterotoxigenic Escherichia coli ( ETEC ) is an endemic health threat in underdeveloped nations . Despite the significant effort extended to vaccine trials using ETEC colonization factors , these approaches have generally not been especially effective in mediating cross-protective immunity . We used quantitative proteo...
Diarrheal disease is an endemic health threat in underdeveloped nations . One of the major causative agents of diarrheal disease is a group of bacteria collectively known as enterotoxigenic Escherichia coli ( ETEC ) . These organisms can cause disease symptoms ranging from mild diarrhea to a more severe , cholera-like ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Protective Enterotoxigenic Escherichia coli Antigens in a Murine Intranasal Challenge Model
Psoriasis is a common inflammatory skin disease characterized by thickened scaly red plaques . Previously we have performed a genome-wide association study ( GWAS ) on psoriasis with 1 , 359 cases and 1 , 400 controls , which were genotyped for 447 , 249 SNPs . The most significant finding was for SNP rs12191877 , whic...
Psoriasis ( Ps ) is a chronic inflammatory disease of the skin , affecting approximately 2% of Europeans . The HLA-C gene , located within the major histocompatibility complex ( MHC ) region on chromosome 6 , is the major genetic determinant of psoriasis . However , multiple susceptibility genes within MHC are also hyp...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "discovery", "dermatology/psoriasis", "and", "other", "inflammatory", "diseases", "genetics", "and", "genomics/complex", "traits", "genetics", "and", "genomics/genetics", "of", "disease", "genetics", "and", "genomics/medical", "genetics" ...
2009
Multiple Loci within the Major Histocompatibility Complex Confer Risk of Psoriasis
One-third of the human population is infected with parasitic worms . To avoid being eliminated , these parasites actively dampen the immune response of their hosts . This immune modulation also suppresses immune responses to third-party antigens such as vaccines . Here , we used Litomosoides sigmodontis-infected BALB/c...
Parasitic worms , called helminths , infect one-third of the world population . Despite exposure to their host's immune system many helminths establish chronic infections and survive several years within their host . They avoid elimination by dampening the immune response of their hosts . This immune suppression also a...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "antibody-producing", "cells", "white", "blood", "cells", "immune", "evasion", "immune", "cells", "cell", "biology", "animal", "cells", "b", "cells", "parasitology", "biology", "and", "life", "sciences", "cellular", "types", "immunology", "immune",...
2014
Nematode-Induced Interference with Vaccination Efficacy Targets Follicular T Helper Cell Induction and Is Preserved after Termination of Infection
A population of dynamic apical actin filaments is required for rapid polarized pollen tube growth . However , the cellular mechanisms driving their assembly remain incompletely understood . It was postulated that formin is a major player in nucleating apical actin assembly , but direct genetic and cytological evidence ...
Actin polymerization has been implicated in the regulation of rapid polarized pollen tube growth . The important role of actin polymerization is well appreciated , but the mechanisms that regulate rapid actin polymerization in pollen tubes remain incompletely understood . It was postulated that one of the major actin p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "anatomy", "cell", "motility", "actin", "filaments", "vesicles", "cell", "processes", "pollen", "plant", "science", "cellular", "structures", "and", "organelles", "dynamic", "actin", "filaments", "contractile", "proteins", "actins", "actin", "polymerization", ...
2018
Arabidopsis class I formins control membrane-originated actin polymerization at pollen tube tips
Dietary restriction extends longevity in organisms ranging from bacteria to mice and protects primates from a variety of diseases , but the contribution of each dietary component to aging is poorly understood . Here we demonstrate that glucose and specific amino acids promote stress sensitization and aging through the ...
Calorie restriction ( CR ) , but also the restriction of specific components of the diet , has been known for decades to affect longevity . However , the understanding of how each component of the macronutrients affects longevity and stress resistance is poorly understood , in part because of the complexity of many of ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "signal", "transduction", "signaling", "in", "cellular", "processes", "model", "organisms", "cellular", "stress", "responses", "adenylyl", "cyclase", "signaling", "pathway", "mechanisms", "of", "signal", "transduction", "genetics", "tor", "signaling", "ras", "signaling"...
2014
Serine- and Threonine/Valine-Dependent Activation of PDK and Tor Orthologs Converge on Sch9 to Promote Aging
Accuracy of transcript quantification with RNA-Seq is negatively affected by positional fragment bias . This article introduces Mix2 ( rd . “mixquare” ) , a transcript quantification method which uses a mixture of probability distributions to model and thereby neutralize the effects of positional fragment bias . The pa...
RNA-Seq is a powerful tool for detecting and quantifying genes and gene isoforms . However , accurate quantification in genomic loci with multiple isoforms has proven difficult . This is due to the fact that the transcript generating an RNA-Seq fragment cannot be identified if multiple transcripts share the fragment se...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "sequencing", "techniques", "engineering", "and", "technology", "molecular", "probe", "techniques", "probability", "distribution", "mathematics", "statistics", "(mathematics)", "industrial", "engineering", "quality", "control", "forms", "of", "dna", "bioassays", "and", "p...
2017
Mixture models reveal multiple positional bias types in RNA-Seq data and lead to accurate transcript concentration estimates
Oral cholera vaccination is an approach to preventing outbreaks in at-risk settings and controlling cholera in endemic settings . However , vaccine-derived herd immunity may be short-lived due to interactions between human mobility and imperfect or waning vaccine efficacy . As the supply and utilization of oral cholera...
Cholera vaccination can be a relatively quick means to temporarily prevent cholera from spreading in an at-risk population . In order to understand how long this temporary protection remains and therefore the timeline for when we need to install longer-term water and sanitation solutions , we must know how long we can ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "immunology", "tropical", "diseases", "geographical", "locations", "south", "sudan", "vaccines", "preventive", "medicine", "bacterial", "diseases", "animal", "behavior", "neglected", "tropical", "diseases", "infectious", "disease",...
2018
Prolonging herd immunity to cholera via vaccination: Accounting for human mobility and waning vaccine effects
Translesion DNA synthesis ( TLS ) is a DNA damage tolerance mechanism in which specialized low-fidelity DNA polymerases bypass replication-blocking lesions , and it is usually associated with mutagenesis . In Saccharomyces cerevisiae a key event in TLS is the monoubiquitination of PCNA , which enables recruitment of th...
DNA damage can block replication and lead to mutations , genomic instability , and cancer . In cases when the removal of DNA damage and restoration of the original sequence prior to replication is impossible , cells utilize DNA damage tolerance mechanisms , which help replication to bypass the lesions . A major univers...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2011
PCNA Ubiquitination Is Important, But Not Essential for Translesion DNA Synthesis in Mammalian Cells
The genus Paracoccidioides consists of thermodymorphic fungi responsible for Paracoccidioidomycosis ( PCM ) , a systemic mycosis that has been registered to affect ~10 million people in Latin America . Biogeographical data subdivided the genus Paracoccidioides in five divergent subgroups , which have been recently clas...
The genus Paracoccidioides comprises fungi responsible for Paracoccidioidomycosis ( PCM ) , a neglected tropical disease prevalent in South America that has been shown to affect approximately 10 million people and has great medical/social impact , since available treatments are poorly effective , frequently leading to ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "fungal", "genetics", "fungi", "genome", "analysis", "genome", "annotation", "research", "and", "analysis", "methods", "paracoccidioides", "mycology", "genomics", "biological", "databases", "comparative", "genomics", "fungal", "genomics", "eukaryota", "database", "and", ...
2019
ParaDB: A manually curated database containing genomic annotation for the human pathogenic fungi Paracoccidioides spp.
Previous game-theoretic studies of vaccination behavior typically have often assumed that populations are homogeneously mixed and that individuals are fully rational . In reality , there is heterogeneity in the number of contacts per individual , and individuals tend to imitate others who appear to have adopted success...
Both infectious diseases and behavioral traits can spread via social contacts . Using network-based mathematical models , our study addresses the interplay between these two processes , as disease spreads through a population and individuals copy their social contacts when making vaccination decisions . Imitation can p...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "game", "theory", "medicine", "public", "health", "and", "epidemiology", "mathematics", "epidemiology", "infectious", "disease", "epidemiology", "applied", "mathematics", "biology", "computational", "biology", "public", "health" ]
2012
The Impact of Imitation on Vaccination Behavior in Social Contact Networks
Hybrid incompatibilities ( HIs ) cause reproductive isolation between species and thus contribute to speciation . Several HI genes encode adaptively evolving proteins that localize to or interact with heterochromatin , suggesting that HIs may result from co-evolution with rapidly evolving heterochromatic DNA . Little i...
Sister species capable of mating often produce hybrids that are sterile or die during development . This reproductive isolation is caused by incompatibilities between the two sister species' genomes . Some hybrid incompatibilities involve genes that encode rapidly evolving proteins that localize to heterochromatin . He...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "biology", "evolutionary", "biology", "genomic", "evolution", "evolutionary", "genetics" ]
2014
The Hmr and Lhr Hybrid Incompatibility Genes Suppress a Broad Range of Heterochromatic Repeats
Functional connectivity concerns the correlated activity between neuronal populations in spatially segregated regions of the brain , which may be studied using functional magnetic resonance imaging ( fMRI ) . This coupled activity is conveniently expressed using covariance , but this measure fails to distinguish betwee...
Significant neuroscientific effort is devoted to elucidating functional connectivity between spatially segregated brain regions . This requires that we are able to quantify the degree of dependence between the signals of different areas . Yet how this must be accomplished—using which measures , each with their own limi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Bayesian Estimation of Conditional Independence Graphs Improves Functional Connectivity Estimates
Fibrillin-1 is a ubiquitous extracellular matrix molecule that sequesters latent growth factor complexes . A role for fibrillin-1 in specifying tissue microenvironments has not been elucidated , even though the concept that fibrillin-1 provides extracellular control of growth factor signaling is currently appreciated ....
The microenvironment is specified by cell-surface molecules , growth factors , and the extracellular matrix . Here we report genetic evidence that implicates fibrillin-1 , a ubiquitous extracellular matrix molecule that sequesters latent growth factor complexes , as a key determinant in the local control of musculoskel...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "biochemistry", "histology", "dermatology", "clinical", "genetics", "model", "organisms", "ophthalmology", "genetics", "biology", "molecular", "cell", "biology", "genetics", "and", "genomics", "cardiovascular" ]
2012
Microenvironmental Regulation by Fibrillin-1
Theileria annulata is an apicomplexan parasite that infects and transforms bovine macrophages that disseminate throughout the animal causing a leukaemia-like disease called tropical theileriosis . Using deep RNAseq of T . annulata-infected B cells and macrophages we identify a set of microRNAs induced by infection , wh...
Theileria annulata-infected bovine macrophages lose their hyper-disseminating virulent phenotype during long-term culture and are used as attenuated live vaccines to fight tropical theileriosis . Deep microRNA sequencing revealed that infection of both B cells and macrophages alters the expression of a large number of ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "transfection", "phosphorylation", "medicine", "and", "health", "sciences", "immune", "cells", "chemical", "compounds", "gene", "regulation", "immunology", "organic", "compounds", "micrornas", "tyrosine", "immunoprecipitation", "amino", "acids", "molecul...
2018
miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence
Resistance and susceptibility to Leishmania major infection in the murine model is determined by the capacity of the host to mount either a protective Th1 response or a Th2 response associated with disease progression . Previous reports involving the use of cysteine cathepsin inhibitors indicated that cathepsins B ( Ct...
The emergence of resistance to the available drugs against cutaneous leishmaniasis emphasizes the need of new chemotherapeutic approaches . Cysteine proteases from Leishmania are important virulence factors and , therefore , interesting drug targets . Studies on inhibitors against these enzymes during Leishmania major ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "dendritic", "cells", "white", "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "protozoans", "cell", "biology", "animal", "cells", "t", "cells", "antigen-presenting", "cells", "biology", "and", "life", "sciences", "c...
2014
Cathepsin B in Antigen-Presenting Cells Controls Mediators of the Th1 Immune Response during Leishmania major Infection
In tropical Africa , where the spectrum of the bacterial pathogens that cause fevers is poorly understood and molecular-based diagnostic laboratories are rare , the time lag between test results and patient care is a critical point for treatment of disease . We implemented POC laboratory in rural Senegal to resolve the...
In tropical Africa , clinical laboratories capable of performing complicated diagnostic studies like PCR are rare and are almost always found in large cities . Moreover , a number of infectious diseases , many of them are emerging and neglected , may be quickly and reliably diagnosed only by molecular biology . This is...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "borrelia", "infection", "public", "health", "and", "epidemiology", "infectious", "disease", "epidemiology", "microbiology", "rickettsia", "relapsing", "fever", "bartonellosis", "bacterial", "diseases", "emerging", "infectious", "diseases", "neglected", "tropica...
2013
Point-of-Care Laboratory of Pathogen Diagnosis in Rural Senegal
Genomic tools have revealed genetically diverse pathogens within some hosts . Within-host pathogen diversity , which we refer to as “complex infection” , is increasingly recognized as a determinant of treatment outcome for infections like tuberculosis . Complex infection arises through two mechanisms: within-host mutat...
Within-host heterogeneity of an infection can arise through two distinct mechanisms: within-host mutation and reinfection . While current genotyping techniques based on MLVA ( multiple loci variable-number tandem repeat analysis ) can identify within-host diversity , standard methods for classifying the mechanism drivi...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "optimization", "mathematics", "fungal", "evolution", "molecular", "biology", "techniques", "genotyping", "bacteria", "bacterial", "pathogens", "research", ...
2016
ClassTR: Classifying Within-Host Heterogeneity Based on Tandem Repeats with Application to Mycobacterium tuberculosis Infections
The genetics of lymphoma susceptibility reflect the marked heterogeneity of diseases that comprise this broad phenotype . However , multiple subtypes of lymphoma are observed in some families , suggesting shared pathways of genetic predisposition to these pathologically distinct entities . Using a two-stage GWAS , we t...
B-cell lymphomas comprise several diseases representing aberrant proliferations of immune cells at various stages of maturation . It might be expected that dissimilar subtypes of lymphoma will have different etiologic and pathogenic mechanisms , reflecting the distinct histologic and clinical characteristics of these d...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genome-wide", "association", "studies", "cancer", "genetics", "genome", "scans", "population", "genetics", "genome", "analysis", "tools", "trait", "locus", "analysis", "population", "biology", "genetic", "polymorphism", "biology", "genetics", "genomics", "genetics", "o...
2013
Susceptibility Loci Associated with Specific and Shared Subtypes of Lymphoid Malignancies
Germline stem cell ( GSC ) self-renewal and differentiation into gametes is regulated by both intrinsic factors in the germ line as well as extrinsic factors from the surrounding somatic niche . dWnt4 , in the escort cells of the adult somatic niche promotes GSC differentiation using the canonical β-catenin-dependent t...
Germ line association with the somatic cells is critical for various aspects of germ cell biology , including migration , self-renewal and differentiation . In Drosophila females , soma–germ line association begins during embryogenesis and continues until the mature egg is formed . In the adult , the somatic escort cel...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "reproductive", "system", "rna", "interference", "gonads", "cell", "cycle", "and", "cell", "division", "cell", "processes", "cell", "differentiation", "developmental", "biology", "stem", "cells", "epigenetics", "research", "and...
2018
A switch in the mode of Wnt signaling orchestrates the formation of germline stem cell differentiation niche in Drosophila
The apical complex is the definitive cell structure of phylum Apicomplexa , and is the focus of the events of host cell penetration and the establishment of intracellular parasitism . Despite the importance of this structure , its molecular composition is relatively poorly known and few studies have experimentally test...
Apicomplexan parasites comprise major human pathogens , including the malaria-causing parasites Plasmodium spp . , and Toxoplasma gondii that causes birth defects and neurological disorders . Key to the success of this group was the evolution of the apical complex , a structure at the focus of the events of host cell i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "signal", "transduction", "cell", "biology", "cell", "cycle", "and", "cell", "division", "biology", "and", "life", "sciences", "cell", "processes", "molecular", "cell", "biology", "cellular", "structures", "and", "organelles", "cell", "signaling", "cytoskeleton", "p...
2014
The Apical Complex Provides a Regulated Gateway for Secretion of Invasion Factors in Toxoplasma
The Global Trachoma Mapping Project ( GTMP ) was implemented with the aim of completing the baseline map of trachoma globally . Over 2 . 6 million people were examined in 1 , 546 districts across 29 countries between December 2012 and January 2016 . The aim of the analysis was to estimate the unit cost and to identify ...
There are currently few data sets available to aid programmes in planning and budgeting for population-based surveys in low- and middle- income countries . With the objective of identifying cost drivers and key variables influencing prevalence survey costs , the authors collected expenses incurred during the Global Tra...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "united", "states", "medicine", "and", "health", "sciences", "engineering", "and", "technology", "transportation", "tropical", "diseases", "geographical", "locations", "social", "sciences", "north", "america", "bacterial", "diseases", "research", "design", "data", "mana...
2017
The cost of mapping trachoma: Data from the Global Trachoma Mapping Project
Like many organisms the fungal pathogen Candida albicans senses changes in the environmental CO2 concentration . This response involves two major proteins: adenylyl cyclase and carbonic anhydrase ( CA ) . Here , we demonstrate that CA expression is tightly controlled by the availability of CO2 and identify the bZIP tra...
Skin infection , oral and vaginal thrush , or bloodstream candidiasis are some of the diseases caused by the human pathogen Candida albicans . The high versatility of infection niches reflects the capacity of this yeast to respond to strong variations in its environment such as CO2 concentration . This molecule initiat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "biology" ]
2012
The bZIP Transcription Factor Rca1p Is a Central Regulator of a Novel CO2 Sensing Pathway in Yeast
Schistosomiasis has decreased significantly in prevalence and intensity of infection in China , thus more accurate and sensitive methods are desperately needed for the further control of schistosomiasis . The present work aimed to assess the utility of the loop-mediated isothermal amplification ( LAMP ) for detection o...
Accurate diagnostic tests play a key role in patient management and control of schistosomiasis , especially in China where the prevalence and intensity of Schistosoma japonicum infection is low in recent years . The present study aimed to assess the utility of the loop-mediated isothermal amplification ( LAMP ) assay f...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
DNA Detection of Schistosoma japonicum: Diagnostic Validity of a LAMP Assay for Low-Intensity Infection and Effects of Chemotherapy in Humans
Sensing extracellular changes initiates signal transduction and is the first stage of cellular decision-making . Yet relatively little is known about why one form of sensing biochemistry has been selected over another . To gain insight into this question , we studied the sensing characteristics of one of the biochemica...
Sensing environmental changes is the first step in the process of cellular decision-making , but many different biochemical sensors exist and why one sensor is selected for a particular task over another is not known . Here we study the sensing properties of a simple and generic allosteric sensor to understand the effe...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "systems", "biology", "biochemical", "simulations", "theoretical", "biology", "biology", "computational", "biology", "signaling", "networks" ]
2011
Trade-Offs and Constraints in Allosteric Sensing
Wolbachia are vertically transmitted , obligatory intracellular bacteria that infect a great number of species of arthropods and nematodes . In insects , they are mainly known for disrupting the reproductive biology of their hosts in order to increase their transmission through the female germline . In Drosophila melan...
Many symbiotic bacteria confer fitness benefits to the organisms that they infect . Wolbachia are one of the most widespread intracellular bacteria , infecting a great number of species of insects . Here we show that in the fruit fly Drosophila melanogaster , infection with Wolbachia increases resistance to a natural p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "infectious", "diseases", "virology", "immunology" ]
2008
The Bacterial Symbiont Wolbachia Induces Resistance to RNA Viral Infections in Drosophila melanogaster
Biofilm formation is an important virulence trait of the pathogenic yeast Candida albicans . We have combined gene overexpression , strain barcoding and microarray profiling to screen a library of 531 C . albicans conditional overexpression strains ( ∼10% of the genome ) for genes affecting biofilm development in mixed...
Candida albicans is the most prevalent human fungal pathogen . Its ability to cause disease relies , in part , on the formation of biofilms , a protective structure of highly adherent cells tolerant to antifungal agents and the host immune response . The biofilm is considered as a persistent root of infection , dissemi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biofilms", "cell", "walls", "ecology", "and", "environmental", "sciences", "genomic", "library", "screening", "genomic", "library", "construction", "model", "organisms", "dna", "construction", "molecular", "biology", "techniques", "cellular", "structures", "and", "orga...
2014
Targeted Changes of the Cell Wall Proteome Influence Candida albicans Ability to Form Single- and Multi-strain Biofilms
The vaccinia virus ( VACV ) A41L gene encodes a secreted 30 kDa glycoprotein that is nonessential for virus replication but affects the host response to infection . The A41 protein shares sequence similarity with another VACV protein that binds CC chemokines ( called vCKBP , or viral CC chemokine inhibitor , vCCI ) , a...
As part of the innate immune response ( for example to virus infection ) , the body produces proteins called chemokines , which act by directing white blood cells ( leukocytes ) to the areas of infection and inflammation . Viruses have evolved mechanisms to fight this immune response . Indeed , so important is this nee...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "viruses", "biophysics", "virology" ]
2008
Structure and Function of A41, a Vaccinia Virus Chemokine Binding Protein
Most organisms are simply diamagnetic , while magnetotactic bacteria and migratory animals are among organisms that exploit magnetism . Biogenic magnetization not only is of fundamental interest , but also has industrial potential . However , the key factor ( s ) that enable biogenic magnetization in coordination with ...
Most organisms do not respond to magnetic fields . However , “magnetotactic” bacteria and migratory animals can sense geomagnetic fields and alter their behavior accordingly . These organisms often contain small magnetic particles that may be responsible for sensing magnetic fields . In magnetotactic bacteria , specifi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "organismal", "evolution", "evolutionary", "biology", "synthetic", "biology", "microbiology", "neuroscience", "model", "organisms", "molecular", "genetics", "environmental", "biotechnology", "forms", "of", "evolution", "signaling", "in", "cellular", "proces...
2012
Induction of Biogenic Magnetization and Redox Control by a Component of the Target of Rapamycin Complex 1 Signaling Pathway
Targeting of permissive entry sites is crucial for bacterial infection . The targeting mechanisms are incompletely understood . We have analyzed target-site selection by S . Typhimurium . This enteropathogenic bacterium employs adhesins ( e . g . fim ) and the type III secretion system 1 ( TTSS-1 ) for host cell bindin...
The animal body is protected by physical , chemical and immunological barriers . Identification of “promising” target sites is therefore of importance for any pathogen . This crucial step of the infection is still poorly understood . Here , we have studied target site selection by the flagellated Gram-negative gut path...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "gram", "negative", "biology", "microbiology", "host-pathogen", "interaction", "bacterial", "pathogens" ]
2012
Near Surface Swimming of Salmonella Typhimurium Explains Target-Site Selection and Cooperative Invasion
Power laws , that is , power spectral densities ( PSDs ) exhibiting behavior for large frequencies f , have been observed both in microscopic ( neural membrane potentials and currents ) and macroscopic ( electroencephalography; EEG ) recordings . While complex network behavior has been suggested to be at the root of th...
The common observation of power laws in nature and society , that is , quantities or probabilities that follow distributions , has for long intrigued scientists . In the brain , power laws in the power spectral density ( PSD ) have been reported in electrophysiological recordings , both at the microscopic ( single-neur...
[ "Abstract", "Introduction", "Models", "Results", "Discussion" ]
[ "physics", "computational", "neuroscience", "single", "neuron", "function", "biophysics", "theory", "biology", "and", "life", "sciences", "physical", "sciences", "computational", "biology", "biophysics", "neuroscience", "biophysical", "simulations" ]
2014
Power Laws from Linear Neuronal Cable Theory: Power Spectral Densities of the Soma Potential, Soma Membrane Current and Single-Neuron Contribution to the EEG
Newborn granule cells become functionally integrated into the synaptic circuitry of the adult dentate gyrus after a morphological and electrophysiological maturation process . The molecular mechanisms by which immature neurons and the neurites extending from them find their appropriate position and target area remain l...
Neural stem cells divide and generate new neurons throughout life in the mammalian hippocampus . After a distinct maturation process , newborn neurons become functionally integrated into the preexisting circuitry and appear to participate in hippocampal function , which is critically involved in certain forms of learni...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience" ]
2008
Cdk5 Regulates Accurate Maturation of Newborn Granule Cells in the Adult Hippocampus
The human intestinal microbiota is essential to the health of the host and plays a role in nutrition , development , metabolism , pathogen resistance , and regulation of immune responses . Antibiotics may disrupt these coevolved interactions , leading to acute or chronic disease in some individuals . Our understanding ...
The intestinal microbiota is essential to human health , with effects on nutrition , metabolism , pathogen resistance , and other processes . Antibiotics may disrupt these interactions and cause acute disease , as well as contribute to chronic health problems , although technical challenges have hampered research on th...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "ecology", "microbiology" ]
2008
The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA Sequencing
In natural environments , bacteria often adhere to surfaces where they form complex multicellular communities . Surface adherence is determined by the biochemical composition of the cell envelope . We describe a novel regulatory mechanism by which the bacterium , Caulobacter crescentus , integrates cell cycle and nutri...
Bacteria predominantly exist within surface-attached communities that facilitate metabolic cooperation , sharing of genetic information , and protect cells against stress . The freshwater bacterium , Caulobacter crescentus elaborates an adhesive structure known as the holdfast , which enables surface attachment . We ha...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "gene", "regulation", "microbiology", "bacterial", "biochemistry", "gene", "function", "prokaryotic", "models", "model", "organisms", "molecular", "genetics", "microbial", "growth", "and", "development", "microbial", "physiology", "cell", "adhesion", "caul...
2014
A Cell Cycle and Nutritional Checkpoint Controlling Bacterial Surface Adhesion
The Fanconi Anemia ( FA ) pathway is important for repairing interstrand crosslinks ( ICLs ) between the Watson-Crick strands of the DNA double helix . An initial and essential stage in the repair process is the detection of the ICL . Here , we report the identification of UHRF2 , a paralogue of UHRF1 , as an ICL senso...
Fanconi Anemia is a genetic disease where patients typically have congenital abnormalities , develop bone marrow failure and suffer from cancer predisposition . The cells in patients have a reduced ability to repair a type of DNA damage where the two strands of the DNA double helix are physically linked together , and ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "chemical", "characterization", "protein", "interactions", "hela", "cells", "enzymes", "cell", "cycle", "and", "cell", "division", "biological", "cultures", "cell", "processes", "enzymology", "in", "vivo", "imaging", "dna", "damage", "immunoprecipitation", "cell", "cu...
2018
Identification of UHRF2 as a novel DNA interstrand crosslink sensor protein
We sought to evaluate the relationship between onchocerciasis prevalence and that of epilepsy using available data collected at community level . We conducted a systematic review and meta-regression of available data . Electronic and paper records on subject area ever produced up to February 2008 . We searched for popu...
Epilepsy is particularly common in tropical areas . One main reason is that many endemic infections have neurological consequences . In addition , the medical , social and demographic burden of epilepsy remains substantial in these countries where it is often seen as a contagious condition and where the aetiology is of...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/helminth", "infections", "neurological", "disorders/epilepsy", "infectious", "diseases/neglected", "tropical", "diseases" ]
2009
Epilepsy in Onchocerciasis Endemic Areas: Systematic Review and Meta-analysis of Population-Based Surveys