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APOBEC3G ( A3G ) is a host cytidine deaminase that , in the absence of Vif , restricts HIV-1 replication and reduces the amount of viral DNA that accumulates in cells . Initial studies determined that A3G induces extensive mutation of nascent HIV-1 cDNA during reverse transcription . It has been proposed that this trig...
APOBEC proteins are cell-encoded factors that inhibit the replication of numerous retroviruses , such as HIV-1 , and retrotransposons . In many cases , inhibition is clearly associated with cytidine-to-uridine editing of viral or transposon DNA . On the other hand , a number of studies with particular APOBEC protein/su...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/mechanisms", "of", "resistance", "and", "susceptibility,", "including", "host", "genetics", "virology/immunodeficiency", "viruses", "virology/host", "antiviral", "responses" ]
2008
APOBEC3G Inhibits Elongation of HIV-1 Reverse Transcripts
Clostridium perfringens is a major cause of food poisoning ( FP ) in developed countries . C . perfringens isolates usually induce the gastrointestinal symptoms of this FP by producing an enterotoxin that is encoded by a chromosomal ( cpe ) gene . Those typical FP strains also produce spores that are extremely resistan...
Spores made by pathogenic Bacillus and Clostridium spp . contribute to disease transmission . Clostridium perfringens food poisoning ( FP ) isolates typically produce spores with exceptional resistance to heat and sodium nitrite . This spore resistance probably facilitates FP strain survival in improperly cooked/held f...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "microbiology/applied", "microbiology", "microbiology/microbial", "physiology", "and", "metabolism", "microbiology/medical", "microbiology" ]
2008
A Novel Small Acid Soluble Protein Variant Is Important for Spore Resistance of Most Clostridium perfringens Food Poisoning Isolates
When taking a bloodmeal from humans , tsetse flies can transmit the trypanosomes responsible for sleeping sickness , or human African trypanosomiasis . While it is commonly assumed that humans must enter the normal woodland habitat of the tsetse in order to have much chance of contacting the flies , recent studies sugg...
To explore the nature of houses as venues for the contact between humans and tsetse flies , and hence for the transmission of sleeping sickness , we studied the sex and species composition and physiological condition of samples of tsetse caught in various types of house throughout the day and at different seasons . The...
[ "Abstract", "Introduction", "General", "Methods", "Experiments", "and", "Results", "Discussion" ]
[ "medicine", "biology" ]
2013
A Neglected Aspect of the Epidemiology of Sleeping Sickness: The Propensity of the Tsetse Fly Vector to Enter Houses
The study of the concerted action of hormones and transcription factors is fundamental to understand cell differentiation and pattern formation during organ development . The root apical meristem of Arabidopsis thaliana is a useful model to address this . It has a stem cell niche near its tip conformed of a quiescent o...
In multicellular development , signaling molecules are essential for the organization of cells into complex differentiated tissues . It is widely acknowledged that tissue or cell context is instructive for the specificity of cell behavior responses , but the underlying system-level mechanisms remain unresolved . The dy...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "gene", "regulation", "regulatory", "proteins", "brassica", "dna-binding", "proteins", "hormones", "regulator", "genes", "plant", "science", "model", "organisms", "plant", "hormones", "membrane", "receptor", "signaling", "hormone", "receptor", "signaling", "transcription"...
2017
A dynamic genetic-hormonal regulatory network model explains multiple cellular behaviors of the root apical meristem of Arabidopsis thaliana
The regulatory mechanisms governing the cell cycle progression of hematopoietic stem cells ( HSCs ) are well characterized , but those responsible for the return of proliferating HSCs to a quiescent state remain largely unknown . Here , we present evidence that CD81 , a tetraspanin molecule acutely responsive to prolif...
Hematopoietic stem cells ( HSCs ) remain dormant in the bone marrow until needed to replenish the hematopoietic system , at which point they are stimulated to proliferate extensively , undergoing both regeneration ( self-renewal ) and differentiation . Self-renewal is key to maintaining an adequate HSC reserve , and re...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "molecular", "cell", "biology" ]
2011
CD81 Is Essential for the Re-entry of Hematopoietic Stem Cells to Quiescence following Stress-Induced Proliferation Via Deactivation of the Akt Pathway
Plasmids are extrachromosomal DNA elements of microorganisms encoding beneficial genetic information . They were thought to be equally distributed to daughter cells during cell division . Here we use mathematical modeling to investigate the evolutionary stability of plasmid segregation for high-copy plasmids—plasmids t...
In the last years , it becomes more and more clear that heterogeneity in isogenic bacterial populations is rather the rule than the exception . This observation is interesting as it reveals the complex social life of bacteria , and also because of tremendous practical implications in medicine , biotechnology , and ecol...
[ "Abstract", "Introduction", "Results", "Discussion", "Models" ]
[ "antimicrobials", "cell", "physiology", "medicine", "and", "health", "sciences", "evolutionary", "biology", "cell", "cycle", "and", "cell", "division", "plasmids", "cell", "processes", "drugs", "microbiology", "applied", "mathematics", "cell", "metabolism", "plasmid", ...
2019
Evolutionary model for the unequal segregation of high copy plasmids
Though evidence is mounting that a major function of sleep is to maintain brain plasticity and consolidate memory , little is known about the molecular pathways by which learning and sleep processes intercept . Anaplastic lymphoma kinase ( Alk ) , the gene encoding a tyrosine receptor kinase whose inadvertent activatio...
Animal and human studies suggest that sleep has a profound impact on learning and memory . However , little is known about the molecular pathways linking these phenomena . We report that mutations in the Drosophila Anaplastic lymphoma kinase ( Alk ) gene , an ortholog of a human oncogene ALK , cause increased sleep . A...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Anaplastic Lymphoma Kinase Acts in the Drosophila Mushroom Body to Negatively Regulate Sleep
Protein structure can provide new insight into the biological function of a protein and can enable the design of better experiments to learn its biological roles . Moreover , deciphering the interactions of a protein with other molecules can contribute to the understanding of the protein's function within cellular proc...
Gene expression in all living organisms is regulated by a complex set of events at both transcriptional and posttranscriptional levels . RNA-binding proteins play a key role in posttranscriptional events including splicing , stability , transport , and translation . Nowadays , there is increasing evidence that many oth...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/bioinformatics", "computational", "biology/macromolecular", "structure", "analysis", "molecular", "biology/rna-protein", "interactions" ]
2008
Classifying RNA-Binding Proteins Based on Electrostatic Properties
Eukaryotic adaptation pathways operate within wide-ranging environmental conditions without stimulus saturation . Despite numerous differences in the adaptation mechanisms employed by bacteria and eukaryotes , all require energy consumption . Here , we present two minimal models showing that expenditure of energy by th...
Adaptation is a common feature in sensory systems , well familiar to us from light and dark adaptation of our visual system . Biological cells , ranging from bacteria to complex eukaryotes , including single-cell organisms and human sensory receptors , adopt different strategies to fulfill this property . However , all...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2013
Unraveling Adaptation in Eukaryotic Pathways: Lessons from Protocells
Oropouche Virus is the etiological agent of an arbovirus febrile disease that affects thousands of people and is widespread throughout Central and South American countries . Although isolated in 1950’s , still there is scarce information regarding the virus biology and its prevalence is likely underestimated . In order...
Oropouche Virus causes typical arboviral febrile illness and is widely distributed in tropical region of Americas , mainly Amazon region , associated with cases of encephalitis . 500 , 000 people are estimated to be infected with Oropouche worldwide and some states in Brazil detected higher number of cases among other ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "liver", "respiratory", "infections", "gene", "regulation", "immunology", "rna", "extraction", "microbiology", "pulmonology", "micrornas", "extraction", "techniques", "research", "and", "analysis", "methods", "animal", "cells", "...
2018
MicroRNA and cellular targets profiling reveal miR-217 and miR-576-3p as proviral factors during Oropouche infection
African trypanosomiasis is a chronic debilitating disease affecting the health and economic well-being of many people in developing countries . The pathogenicity associated with this disease involves a persistent inflammatory response , whereby M1-type myeloid cells , including Ly6Chigh inflammatory monocytes , are cen...
Uncontrolled inflammation is a major contributor to pathogenicity development during many chronic parasitic infections , including African trypanosome infections . Hence , therapies should aim at re-establishing the balance between pro- and anti-inflammatory responses to reduce tissue damage . Our experiments uncovered...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "parasitology", "biology", "and", "life", "sciences", "immunology" ]
2014
MIF Contributes to Trypanosoma brucei Associated Immunopathogenicity Development
Sexual transmission of Ebola virus disease ( EVD ) 6 months after onset of symptoms has been recently documented , and Ebola virus RNA has been detected in semen of survivors up to 9 months after onset of symptoms . As countries affected by the 2013–2015 epidemic in West Africa , by far the largest to date , are declar...
Researchers have recently raised suspicion that the Ebola virus can be transmitted sexually from survivors after recovering from the life-threatening acute phase characteristic of Ebola virus disease ( EVD ) . However , the nature of the impact sexual transmission from convalescent survivors may have on disease dynamic...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "infectious", "disease", "epidemiology", "pathogens", "spatial", "epidemiology", "microbiology", "geographical", "locations", "tropical", "diseases", "ebola", "hemorrh...
2016
Potential Impact of Sexual Transmission on Ebola Virus Epidemiology: Sierra Leone as a Case Study
Inference of interaction rules of animals moving in groups usually relies on an analysis of large scale system behaviour . Models are tuned through repeated simulation until they match the observed behaviour . More recent work has used the fine scale motions of animals to validate and fit the rules of interaction of an...
The collective movement of animals in a group is an impressive phenomenon whereby large scale spatio-temporal patterns emerge from simple interactions between individuals . Theoretically , much of our understanding of animal group motion comes from models inspired by statistical physics . In these models , animals are ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "statistical", "mechanics", "applied", "mathematics", "bayes", "theorem", "probability", "distribution", "animal", "behavior", "mathematics", "theoretical", "ecology", "stochastic", "processes", "zoology", "complex", "systems", "theoretical", "biology", "probability", "dens...
2012
Multi-scale Inference of Interaction Rules in Animal Groups Using Bayesian Model Selection
Salmonella enterica serovar Enteritidis is a cause of both poultry- and egg-associated enterocolitis globally and bloodstream-invasive nontyphoidal Salmonella ( iNTS ) disease in sub-Saharan Africa ( sSA ) . Distinct , multi-drug resistant genotypes associated with iNTS disease in sSA have recently been described , oft...
Salmonella enterica serovar Enteritidis is both a prominent global cause of zoonotic gastroenteritis , in association with the industrial production of eggs and poultry , and of bloodstream-invasive infection in sub-Saharan Africa , a clinical syndrome referred to as invasive nontyphoidal Salmonella ( iNTS ) disease . ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "taxonomy", "livestock", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "pathogens", "geographical", "locations", "microbiology", "vertebrates", "diet", "animals", "animal", "products", "bacterial", "diseases", "...
2019
Emergence of phylogenetically diverse and fluoroquinolone resistant Salmonella Enteritidis as a cause of invasive nontyphoidal Salmonella disease in Ghana
Leprosy control is achieved through a fine-tuning of TH1 and TH2 immune response pattern balance . Given the increasing epidemiological overlay of HIV and M . leprae infections , immune response in co-infected patients consists in an important contemporary issue . Here we describe for the first time the innate lymphoid...
Mycobacterium leprae is a clinical relevant pathogen that can lead to leprosy upon infection . This chronic infectious disease is characterized by the appearance of skin and peripheral nerve lesions . Normally , a healthy immune system is able to control the infection and impede the generation of lesions . However , im...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
New Players in the Same Old Game: Disturbance of Group 2 Innate Lymphoid Cells in HIV-1 and Mycobacterium leprae Co-infected Patients
Hosts encounter an ever-changing array of pathogens , so there is continual selection for novel ways to resist infection . A powerful way to understand how hosts evolve resistance is to identify the genes that cause variation in susceptibility to infection . Using high-resolution genetic mapping we have identified a na...
Hosts and their pathogens are engaged in a never-ending arms race , and hosts must continually evolve new defences to protect themselves from infection . In the fruit fly Drosophila melanogaster we show that virus resistance can evolve through a single mutation . In flies that are highly resistant to a naturally occurr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2016
A Polymorphism in the Processing Body Component Ge-1 Controls Resistance to a Naturally Occurring Rhabdovirus in Drosophila
Resistance to allopurinol in zoonotic canine leishmaniasis has been recently shown to be associated with disease relapse in naturally-infected dogs . However , information regarding the formation of resistance and its dynamics is lacking . This study describes the successful in-vitro induction of allopurinol resistance...
Visceral leishmaniasis caused by the parasite Leishmania infantum is a neglected tropical disease transmitted from animal hosts to humans by sand fly bites . This potentially fatal disease affects thousands of people annually and threatens millions who live in disease risk areas . Domestic dogs are considered as the ma...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "microbiology", "vertebrates", "cloning", "parasitic", "diseases", "animals", "protozoan", "life", "cycles", "mammals", "dogs", "parasitic", "protozoans", "developmental", "biology", "protozoans", "leishmania", "genome", "analysis"...
2017
Induction of allopurinol resistance in Leishmania infantum isolated from dogs
A published study used a stochastic branching process to derive equations for the mean and variance of the probability of , and time to , extinction in population of tsetse flies ( Glossina spp ) as a function of adult and pupal mortality , and the probabilities that a female is inseminated by a fertile male . The orig...
We derive equations for the mean and variance of the probability of , and time to , extinction in population of tsetse flies ( Glossina spp ) , the vectors of trypanosomiasis in sub-Saharan Africa . In so doing we provide the complete proofs for all results , which were not provided in a previously published study . We...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[]
2019
Improved estimates for extinction probabilities and times to extinction for populations of tsetse (Glossina spp)
Transition metal ions ( Zn ( II ) , Cu ( II ) / ( I ) , Fe ( III ) / ( II ) , Mn ( II ) ) are essential for life and participate in a wide range of biological functions . Cellular Zn ( II ) levels must be high enough to ensure that it can perform its essential roles . Yet , since Zn ( II ) binds to ligands with high av...
Zinc ( Zn ( II ) ) is often considered to be a “first among equals” in metal ion homeostasis . Zn ( II ) is critically important to the proper function of many cellular processes , yet is toxic at high levels . The molecular basis for Zn ( II ) intoxication is poorly understood . Using a forward genetic approach in B ....
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "heme", "b", "vitamins", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "chemical", "compounds", "gene", "regulation", "pathogens", "bacillus", "microbiology", "organic", "compounds", "toxicology", "toxicity", "prokaryotic", "mode...
2016
Intracellular Zn(II) Intoxication Leads to Dysregulation of the PerR Regulon Resulting in Heme Toxicity in Bacillus subtilis
As obligate blood-feeding arthropods , ticks transmit pathogens to humans and domestic animals more often than other arthropod vectors . Livestock farming plays a vital role in the rural economy of Pakistan , and tick infestation causes serious problems with it . However , research on tick species diversity and tick-bo...
Ticks are known for their negative impact on animal and human health through infestation and are capable of transmitting a wide range of pathogens including protozoan , viruses , and bacteria such as the spirochetes and rickettsiae . Ticks are widely distributed in different ecological , and geographical regions of Pak...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "livestock", "medicine", "and", "health", "sciences", "parasite", "groups", "ixodes", "pathology", "and", "laboratory", "medicine", "ruminants", "pathogens", "geographical", "locations", "microbiology", "vertebrates", "buffaloes", "animals", "mammals", "p...
2017
A study of ticks and tick-borne livestock pathogens in Pakistan
In plants , each male meiotic product undergoes mitosis , and then one of the resulting cells divides again , yielding a three-celled pollen grain comprised of a vegetative cell and two sperm cells . Several genes have been found to act in this process , and DUO1 ( DUO POLLEN 1 ) , a transcription factor , plays a key ...
For all eukaryotes , gamete formation is an essential aspect of sexual reproduction . Unlike in animals , where meiotic products directly become gametes , the germline in plants is established by two consecutive mitotic divisions after meiosis is completed . The first mitosis is asymmetric , forming a larger vegetative...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "pollen", "developmental", "biology", "plant", "anatomy", "plant", "science", "cell", "biology", "fertilization", "gene", "expression", "genetics", "biology", "and", "life", "sciences", "molecular", "cell", "biology", "flowering", "plants", "plants", "organisms" ]
2014
An ARID Domain-Containing Protein within Nuclear Bodies Is Required for Sperm Cell Formation in Arabidopsis thaliana
The life cycle of the mammalian pathogen Trypanosoma brucei involves commuting between two markedly different environments: the homeothermic mammalian host and the poikilothermic invertebrate vector . The ability to resist temperature and other stresses is essential for trypanosome survival . Trypanosome gene expressio...
Like other organisms , the mammalian pathogen Trypanosoma brucei is able to sense environmental changes and to change its gene expression accordingly . In contrast with other organisms , however , trypanosomes and related kinetoplastids effect these changes almost exclusively by controlling the translation of mRNAs int...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "Methods" ]
[ "phosphorylation", "rna-binding", "proteins", "cellular", "stress", "responses", "rna", "interference", "messenger", "rna", "cell", "processes", "polyribosomes", "immunoprecipitation", "epigenetics", "cellular", "structures", "and", "organelles", "research", "and", "analysi...
2016
Regulating a Post-Transcriptional Regulator: Protein Phosphorylation, Degradation and Translational Blockage in Control of the Trypanosome Stress-Response RNA-Binding Protein ZC3H11
The fungus Paracoccidioides lutzii was recently included as a new causative species of paracoccidioidomycosis ( PCM ) and most cases have been reported from Brazil . According to available epidemiological information , P . lutzii is concentrated in the Middle-West region in Brazil , mainly in the state of Mato Grosso ....
Paracoccidioidomycosis ( PCM ) is an endemic mycosis in Latin America with high incidence in Brazil . The fungi Paracoccidioides brasiliensis ( including genetic groups S1 , PS2 , PS3 and PS4 ) and Paracoccidioides lutzii are the etiological agents , but little is known about the clinical manifestations of PCM caused b...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "respiratory", "infections", "pathogens", "tropical", "diseases", "microbiology", "geographical", "locations", "pulmonology", "fungi", "lymph", "nodes", "signs", "and", "symptoms", "l...
2019
Clinical and epidemiological features of paracoccidioidomycosis due to Paracoccidioides lutzii
Cellular receptors can act as molecular switches , regulating the sensitivity of microbial proteins to conformational changes that promote cellular entry . The activities of these receptor-based switches are only partially understood . In this paper , we sought to understand the mechanism that underlies the activity of...
The bacterium that causes anthrax produces a toxin called anthrax toxin that is largely responsible for causing disease symptoms . The first step in anthrax intoxication involves binding of the toxin to a specific protein , called a receptor , on the cell surface . Receptor-binding acts like a switch to prevent the tox...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "protein", "interactions", "proteins", "biology" ]
2011
A Receptor-based Switch that Regulates Anthrax Toxin Pore Formation
Networks of interacting transcription factors are central to the regulation of cellular responses to abiotic stress . Although the architecture of many such networks has been mapped , their dynamic function remains unclear . Here we address this challenge in archaea , microorganisms possessing transcription factors tha...
Complex circuits of genes rather than a single gene underlie many important processes such as disease , development , and cellular damage repair . Although the wiring of many of these circuits has been mapped , how circuits operate in real time to carry out their functions is poorly understood . Here we address these q...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "genetics", "biology", "and", "life", "sciences", "microbiology", "computational", "biology", "molecular", "biology" ]
2015
A Regulatory Hierarchy Controls the Dynamic Transcriptional Response to Extreme Oxidative Stress in Archaea
The hemolytic phospholipase C ( PlcHR ) expressed by Pseudomonas aeruginosa is the original member of a Phosphoesterase Superfamily , which includes phosphorylcholine-specific phospholipases C ( PC-PLC ) produced by frank and opportunistic pathogens . PlcHR , but not all its family members , is also a potent sphingomye...
Pseudomonas aeruginosa is a major bacterial opportunistic pathogen responsible for acute ( e . g . , sepsis ) and chronic infections ( e . g . , pulmonary ) . While it expresses assorted extracellular toxins that in one way or another contribute to pathogenesis , the precise cellular and molecular mechanisms by which t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/nosocomial", "and", "healthcare-associated", "infections", "cell", "biology/cell", "signaling", "biochemistry/protein", "chemistry", "genetics", "and", "genomics/disease", "models", "infectious", "diseases/bacterial", "infections", "microbiology/cellular", ...
2009
A Complex Extracellular Sphingomyelinase of Pseudomonas aeruginosa Inhibits Angiogenesis by Selective Cytotoxicity to Endothelial Cells
ClinicalTrials . gov NCT03173742 . The control of Neglected Tropical Diseases ( NTDs ) has in ivermectin ( IVM ) the most significant tool among all the drugs used for morbidity control and interruption of transmision . Due to its impact on onchocerciasis and lymphatic filariasis ( LF ) , this macrocyclic lactone has b...
Current efforts for the control of poverty-related diseases provide drug treatments through mass drug administration ( MDA ) as a key component . Ivermectin is an antiparasitary drug which has been used to fight some of these diseases , and millions of treatments have been distributed with a favorable toxicity profile ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "pharmacologic", "analysis", "body", "weight", "medicine", "and", "health", "sciences", "body", "fluids", "clinical", "research", "design", "diet", "research", "design", "physiological", "parameters", "nutrition", "pharmaceutics", "drug", "administration", "pharmacology",...
2018
Safety and pharmacokinetic profile of fixed-dose ivermectin with an innovative 18mg tablet in healthy adult volunteers
The murine model of experimental cerebral malaria ( ECM ) has been utilised extensively in recent years to study the pathogenesis of human cerebral malaria ( HCM ) . However , it has been proposed that the aetiologies of ECM and HCM are distinct , and , consequently , no useful mechanistic insights into the pathogenesi...
Cerebral malaria ( HCM ) is the most severe complication of malaria infection . Despite this , we have an incomplete understanding of the cause ( pathogenesis ) of the syndrome . To improve our understanding of HCM pathogenesis , animal models of the syndrome have been developed . The most commonly used model is the mu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "cell", "death", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "cardiovascular", "anatomy", "immunology", "tropical", "diseases", "cell", "processes", "parasitic", "diseases", "capillaries", ...
2017
A quantitative brain map of experimental cerebral malaria pathology
Plasmodesmata provide the cytoplasmic conduits for cell-to-cell communication throughout plant tissues and participate in a diverse set of non–cell-autonomous functions . Despite their central role in growth and development and defence , resolving their modus operandi remains a major challenge in plant biology . Featur...
In plants , cylindrical , microscopic channels called plasmodesmata provide intracellular connections between cells for communication and material transport , and are important for many aspects of plant growth and defence . We identify a novel family of plasmodesmata-located proteins ( called PDLP1 ) with features of t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "biology" ]
2008
Specific Targeting of a Plasmodesmal Protein Affecting Cell-to-Cell Communication
The Drosophila eye is a mosaic that results from the stochastic distribution of two ommatidial subtypes . Pale and yellow ommatidia can be distinguished by the expression of distinct rhodopsins and other pigments in their inner photoreceptors ( R7 and R8 ) , which are implicated in color vision . The pale subtype conta...
Most sensory systems follow the rule “one receptor molecule per receptor cell . ” For example , photoreceptors in the fly eye and cones in the human eye each express only one light-sensitive rhodopsin . Rhodopsins are G-coupled protein receptors , a class of ancient signaling molecules that mediate not just vision but ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience" ]
2008
Iroquois Complex Genes Induce Co-Expression of rhodopsins in Drosophila
Epoxyeicosatrienoic acids ( EETs ) confer vasoactive and cardioprotective functions . Genetic analysis of the contributions of these short-lived mediators to pathophysiology has been confounded to date by the allelic expansion in rodents of the portion of the genome syntenic to human CYP2J2 , a gene encoding one of the...
In mice and humans , the CYP2J class of cytochrome P450 epoxygenases metabolizes arachidonic acid ( AA ) to epoxyeicosatrienoic acids ( EETs ) , short-lived mediators with effects on both the pulmonary and systemic vasculature . Genetic dissection of CYP2J function to date has been complicated by allelic expansion in t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Deletion of the Murine Cytochrome P450 Cyp2j Locus by Fused BAC-Mediated Recombination Identifies a Role for Cyp2j in the Pulmonary Vascular Response to Hypoxia
The neural precursor cell expressed developmentally down-regulated gene 4–2 , Nedd4-2 , is an epilepsy-associated gene with at least three missense mutations identified in epileptic patients . Nedd4-2 encodes a ubiquitin E3 ligase that has high affinity toward binding and ubiquitinating membrane proteins . It is curren...
Many patients with neurological disorders suffer from an imbalance in neuronal and circuit excitability and present with seizure or epilepsy as the common comorbidity . Human genetic studies have identified many epilepsy-associated genes , but the pathways by which those genes are connected to brain circuit excitabilit...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "action", "potentials", "medicine", "and", "health", "sciences", "hek", "293", "cells", "membrane", "potential", "biological", "cultures", "electrophysiology", "neuroscience", "animal", "models", "mutation", "model", "organisms", "immunoprecipitation", "experimental", "or...
2017
Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors
Ecological speciation is the process by which reproductively isolated populations emerge as a consequence of divergent natural or ecologically-mediated sexual selection . Most genomic studies of ecological speciation have investigated allopatric populations , making it difficult to infer reproductive isolation . The fe...
Ecological speciation can be defined as the evolution of new , reproductively isolated , species driven by natural selection and ecologically-mediated sexual selection . Its genomic signature has mainly been studied in ecotypes and emerging species that started diverging hundreds to thousands of generations ago , while...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "ecology", "and", "environmental", "sciences", "population", "genetics", "vertebrates", "animals", "osteichthyes", "aquatic", "environments", "habitats", "speciation", "bodies", "of", "water", "dna", "recombination", "dna", "population", "biology", "fishes", "chromosome",...
2016
Genomics of Rapid Incipient Speciation in Sympatric Threespine Stickleback
Several low-grade persistent viral infections induce and sustain very large numbers of virus-specific effector T cells . This was first described as a response to cytomegalovirus ( CMV ) , a herpesvirus that establishes a life-long persistent/latent infection , and sustains the largest known effector T cell populations...
Herpesviruses persist for the life of the host and must be continuously controlled by a robust immune surveillance effort . In the case of the cytomegalovirus ( CMV ) , this ongoing immune surveillance promotes the accumulation of CMV-specific T cells in a process known as “memory inflation” . We and others have propos...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "animal", "models", "of", "infection", "white", "blood", "cells", "immune", "cells", "cell", "biology", "animal", "cells", "immunity", "viral", "persistence", "and", "latency", "virology", "t", "cells", "biology", "and", "life", "sciences", "ce...
2014
Systemic Hematogenous Maintenance of Memory Inflation by MCMV Infection
Our ability to identify genes that participate in cell growth and division is limited because their loss often leads to lethality . A solution to this is to isolate conditional mutants where the phenotype is visible under restrictive conditions . Here , we capitalize on the haploid growth-phase of the moss Physcomitrel...
Genes important for cell growth are difficult to identify because their disruption often results in the death of the organism . A solution to this problem is to isolate temperature-sensitive mutants where growth is blocked only at high temperatures . Here , we used the moss Physcomitrella patens , a simple model plant ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "microtubules", "microtubule", "dynamics", "cell", "processes", "organisms", "mutation", "chromosome", "mapping", "microtubule", "polymerization", "molecular", "biology", "techniques", "plants", "cellular", "structures", "and", "organelles", "nonvascular", "plants", "cytosk...
2018
Conditional genetic screen in Physcomitrella patens reveals a novel microtubule depolymerizing-end-tracking protein
Although a combination of genomic and epigenetic alterations are implicated in the multistep transformation of normal squamous esophageal epithelium to Barrett esophagus , dysplasia , and adenocarcinoma , the combinatorial effect of these changes is unknown . By integrating genome-wide DNA methylation , copy number , a...
The incidence of esophageal adenocarcinoma ( EA ) is increasing at an alarming pace in the United States . Distinct pathological stages of Barrett's metaplasia and low- and high-grade dysplasia can be seen preceding malignant transformation . These precursor lesions provide a unique in vivo model for deepening our unde...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "gastroenterology", "and", "hepatology/gastrointestinal", "cancers", "genetics", "and", "genomics/epigenetics", "gastroenterology", "and", "hepatology/esophagus", "oncology/gastrointestinal", "cancers" ]
2011
Widespread Hypomethylation Occurs Early and Synergizes with Gene Amplification during Esophageal Carcinogenesis
Recent studies have identified broadband phenomena in the electric potentials produced by the brain . We report the finding of power-law scaling in these signals using subdural electrocorticographic recordings from the surface of human cortex . The power spectral density ( PSD ) of the electric potential has the power-...
For a very long time , the measurement of the large scale potentials produced by the brain from outside of the head , using electroencephalography and magnetoencephalography , and from inside the head , using electrocorticography , has fixated on changes in specific rhythms and frequency ranges . This fixation presuppo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/motor", "systems", "neuroscience", "neuroscience/theoretical", "neuroscience", "physics/condensed", "matter" ]
2009
Power-Law Scaling in the Brain Surface Electric Potential
To protect germ cells from genomic instability , surveillance mechanisms ensure meiosis occurs properly . In mammals , spermatocytes that display recombination defects experience a so-called recombination-dependent arrest at the pachytene stage , which relies on the MRE11 complex—ATM—CHK2 pathway responding to unrepair...
Meiosis is a specialized cell division that generates haploid gametes by halving chromosome content through two consecutive rounds of chromosome segregation . At the onset of the first meiotic division , SPO11 protein introduces double-strand breaks ( DSBs ) throughout the genome . These DSBs are repaired through homol...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "death", "spermatocytes", "nuclear", "staining", "cell", "processes", "germ", "cells", "oocytes", "dna", "sperm", "research", "and", "analysis", "methods", "specimen", "preparation", "and", "treatment", "staining", "animal", "cells", "proteins", "recombinant",...
2017
p53 and TAp63 participate in the recombination-dependent pachytene arrest in mouse spermatocytes
Toxoplasma gondii is the most common protozoan parasitic infection in man . Gamma interferon ( IFNγ ) activates haematopoietic and non-haematopoietic cells to kill the parasite and mediate host resistance . IFNγ-driven host resistance pathways and parasitic virulence factors are well described in mice , but a detailed ...
Toxoplasma gondii is an intracellular parasite that can invade nucleated cells of any warm-blooded animal into a compartment known as a parasitophorous vacuole ( PV ) . The production of gamma interferon ( IFNγ ) drives the restriction and killing of Toxoplasma . It is not fully known how the parasite inside the PV is ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "cell", "death", "medicine", "and", "health", "sciences", "autophagic", "cell", "death", "vacuoles", "parasite", "replication", "pathology", "and", "laboratory", "medicine", "hela", "cells", "cell", "processes", "biological", "cultures", "parasitic", "diseases", "para...
2016
K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFNγ-Stimulated Human Cells
The emergence and re-emergence of pathogens remains a major public health concern . Unfortunately , when and where pathogens will ( re- ) emerge is notoriously difficult to predict , as the erratic nature of those events is reinforced by the stochastic nature of pathogen evolution during the early phase of an epidemic ...
The probability that an epidemic will break out is highly dependent on the ability of the pathogen to acquire new adaptive mutations and to induce evolutionary emergence . Forecasting pathogen emergence thus requires a good understanding of the interplay between the epidemiology and evolution taking place at the onset ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome", "engineering", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "pathology", "and", "laboratory", "medicine", "engineering", "and", "technology", "bacteriophages", "pathogens", "synthetic", "biology", "synthetic", "bioengin...
2018
Evolutionary emergence of infectious diseases in heterogeneous host populations
Nonalcoholic fatty liver disease ( NAFLD ) clusters in families , but the only known common genetic variants influencing risk are near PNPLA3 . We sought to identify additional genetic variants influencing NAFLD using genome-wide association ( GWA ) analysis of computed tomography ( CT ) measured hepatic steatosis , a ...
NAFLD is a spectrum of disease that ranges from steatosis to steatohepatitis ( nonalcoholic steatohepatitis or NASH: inflammation around the fat ) to fibrosis/cirrhosis . Hepatic steatosis can be measured non-invasively using computed tomography ( CT ) whereas NASH/fibrosis is assessed histologically . The genetic unde...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/complex", "traits", "diabetes", "and", "endocrinology/obesity", "gastroenterology", "and", "hepatology/hepatology" ]
2011
Genome-Wide Association Analysis Identifies Variants Associated with Nonalcoholic Fatty Liver Disease That Have Distinct Effects on Metabolic Traits
Persistent activity has been reported in many brain areas and is hypothesized to mediate working memory and emotional brain states and to rely upon network or biophysical feedback . Here , we demonstrate a novel mechanism by which persistent neuronal activity can be generated without feedback , relying instead on the s...
The accessory olfactory system is essential for chemical communication in animals during social interactions . During this process , the principle cells of the accessory olfactory bulb ( AOB ) may respond to transient stimulation with prolonged activity , sometimes lasting for minutes—a property known as persistent act...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Prolonged Intracellular Na+ Dynamics Govern Electrical Activity in Accessory Olfactory Bulb Mitral Cells
The interactions between membrane receptors and extracellular ligands control cell-cell and cell-substrate adhesion , and environmental responsiveness by representing the initial steps of cell signaling pathways . These interactions can be spatial-temporally regulated when different extracellular ligands are tethered ....
In order to adapt to surrounding environments , multiple signaling pathways have been evolved in cells . The first step of these pathways is to detect external stimuli , which is conducted by the dynamic interactions between cell surface receptors and extracellular ligands . As a result , recognition of extracellular l...
[ "Abstract", "Introduction", "Model", "and", "method", "Results", "Discussion" ]
[ "cell", "binding", "biomacromolecule-ligand", "interactions", "cell", "physiology", "chemical", "characterization", "simulation", "and", "modeling", "membrane", "proteins", "membrane", "receptor", "signaling", "receptor-ligand", "binding", "assay", "cellular", "structures", ...
2017
General principles of binding between cell surface receptors and multi-specific ligands: A computational study
The species-specific phenolic glycolipid 1 ( PGL-1 ) is suspected to play a critical role in the pathogenesis of leprosy , a chronic disease of the skin and peripheral nerves caused by Mycobacterium leprae . Based on studies using the purified compound , PGL-1 was proposed to mediate the tropism of M . leprae for the n...
Mycobacterium leprae , the causative agent of leprosy , is a chronic human disease responsible for irreversible peripheral nerve damage and deformities . Lepromatous leprosy , the most severe form of the disease , is accompanied by T-cell unresponsiveness , suggesting that M . leprae has evolved strategies to modulate ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/bacterial", "infections", "microbiology/cellular", "microbiology", "and", "pathogenesis", "microbiology/innate", "immunity", "genetics", "and", "genomics/functional", "genomics" ]
2010
Mycobacterium leprae Phenolglycolipid-1 Expressed by Engineered M. bovis BCG Modulates Early Interaction with Human Phagocytes
In all models , but especially in those used to predict uncertain processes ( e . g . , climate change and nonnative species establishment ) , it is important to identify and remove any sources of bias that may confound results . This is critical in models designed to help support decisionmaking . The geometry used to ...
Many different areas of science try to simulate and predict ( model ) how processes act across virtual landscapes . Sometimes these models are abstract , but often they are based on real-world landscapes and are used to make real-world planning or management decisions . We considered two separate issues: how movement o...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "none", "ecology", "science", "policy" ]
2007
Landscape as a Model: The Importance of Geometry
Genetic causes for autosomal recessive forms of dilated cardiomyopathy ( DCM ) are only rarely identified , although they are thought to contribute considerably to sudden cardiac death and heart failure , especially in young children . Here , we describe 11 young patients ( 5–13 years ) with a predominant presentation ...
Idiopathic dilated cardiomyopathy ( DCM ) is estimated to be of genetic origin in 20%–48% of the patients . Almost all currently known genetic defects show dominant inheritance , although especially in younger children recessive causes have been proposed to contribute considerably to DCM . Knowledge of the genetic caus...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "autosomal", "recessive", "diagnostic", "medicine", "pediatric", "cardiology", "pediatrics", "and", "child", "health", "genetics", "and", "genomics", "metabolic", "disorders", "pediatrics", "clinical", "genetics" ]
2011
Autosomal Recessive Dilated Cardiomyopathy due to DOLK Mutations Results from Abnormal Dystroglycan O-Mannosylation
Mechanisms for highly efficient chromosome-associated equal segregation , and for maintenance of steady state copy number , are at the heart of the evolutionary success of the 2-micron plasmid as a stable multi-copy extra-chromosomal selfish DNA element present in the yeast nucleus . The Flp site-specific recombination...
Plasmids of budding yeasts , exemplified by the 2-micron plasmid of Saccharomyces cerevisiae , and mammalian papilloma and gammaherpes viruses typify eukaryotic extra-chromosomal selfish DNA elements . The plasmid and the viral episomes , despite the long evolutionary divergence of their hosts , share striking similari...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "recombination", "reactions", "monomers", "plasmids", "dna-binding", "proteins", "plasmid", "construction", "sumoylation", "dna", "replication", "genetic", "elements", "forms", "of", "dna", "dna", "construction", "dna", "molecular", "biology", "techniques", "research", ...
2019
A Flp-SUMO hybrid recombinase reveals multi-layered copy number control of a selfish DNA element through post-translational modification
Imprinted genes undergo epigenetic modifications during gametogenesis , which lead to transcriptional silencing of either the maternally or the paternally derived allele in the subsequent generation . Previous work has suggested an association between imprinting and the products of retrotransposition , but the nature o...
The conventional view is that DNA carries all of our heritable information and our genes control development into adulthood . The discovery of epigenetics , a term coined to describe effects that are not coded for by DNA sequence , but can nonetheless affect our development and well-being , has added another layer of c...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "cell", "biology", "evolutionary", "biology", "homo", "(human)", "genetics", "and", "genomics", "mus", "(mouse)" ]
2007
A Screen for Retrotransposed Imprinted Genes Reveals an Association between X Chromosome Homology and Maternal Germ-Line Methylation
Genome-wide association ( GWA ) is gaining popularity as a means to study the architecture of complex quantitative traits , partially due to the improvement of high-throughput low-cost genotyping and phenotyping technologies . Glucosinolate ( GSL ) secondary metabolites within Arabidopsis spp . can serve as a model sys...
Understanding how genetic variation can control phenotypic variation is a fundamental goal of modern biology . A major push has been made using genome-wide association mapping in all organisms to attempt and rapidly identify the genes contributing to phenotypes such as disease and nutritional disorders . But a number o...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome-wide", "association", "studies", "functional", "genomics", "plant", "biology", "population", "genetics", "metabolic", "networks", "plant", "science", "genome", "complexity", "genetic", "polymorphism", "plant", "genetics", "biology", "systems", "biology", "plant", ...
2011
Combining Genome-Wide Association Mapping and Transcriptional Networks to Identify Novel Genes Controlling Glucosinolates in Arabidopsis thaliana
Neurocysticercosis is a leading cause of preventable epilepsy in the developing world . Sustainable community-based interventions are urgently needed to control transmission of the causative parasite , Taenia solium . We examined the geospatial relationship between live pigs with visible cysticercotic cysts on their to...
Taenia solium , aka the pork tapeworm , is an important cause of epilepsy in developing nations . People with intestinal tapeworms , a condition known as taeniasis , pass infectious eggs in their feces which contaminate the environment . These eggs can cause serious disease in both humans and pigs if they are ingested ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "public", "health", "and", "epidemiology", "neurocysticercosis", "infectious", "disease", "epidemiology", "parasitic", "diseases", "global", "health", "neglected", "tropical", "diseases", "infectious", "disease", "control", "veterinary", "science", "infectious",...
2012
Geographic Correlation between Tapeworm Carriers and Heavily Infected Cysticercotic Pigs
Kaposi's sarcoma-associated herpesvirus ( KSHV ) is causally linked to several human cancers , including Kaposi's sarcoma , primary effusion lymphoma and multicentric Castleman's disease , malignancies commonly found in HIV-infected patients . While KSHV encodes diverse functional products , its mechanism of oncogenesi...
Kaposi's sarcoma-associated herpesvirus ( KSHV ) is the causal agent of several human cancers . KSHV encodes over two dozen genes that regulate diverse cellular pathways . However , the molecular mechanism of KSHV-induced oncogenesis remains unknown . In this study , we determined the roles of KSHV microRNAs ( miRs ) i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
KSHV MicroRNAs Mediate Cellular Transformation and Tumorigenesis by Redundantly Targeting Cell Growth and Survival Pathways
Prions are proteinaceous infectious agents responsible for fatal neurodegenerative diseases in animals and humans . They are essentially composed of PrPSc , an aggregated , misfolded conformer of the ubiquitously expressed host-encoded prion protein ( PrPC ) . Stable variations in PrPSc conformation are assumed to enco...
Prions are infectious agents causing irremediably fatal neurodegenerative diseases in human and in farmed or wild animals . They are thought to be formed from abnormally folded assemblies ( PrPSc ) of the host-encoded prion protein ( PrPC ) . Different PrPSc conformational variants associated with distinct biological p...
[ "Abstract", "Introduction", "Material", "and", "Methods", "Results", "Discussion" ]
[]
2013
Quaternary Structure of Pathological Prion Protein as a Determining Factor of Strain-Specific Prion Replication Dynamics
Alternative mRNA splicing adds a layer of regulation to the expression of thousands of genes in Drosophila melanogaster . Not all alternative splicing results in functional protein; it can also yield mRNA isoforms with premature stop codons that are degraded by the nonsense-mediated mRNA decay ( NMD ) pathway . This co...
A gene can be processed into multiple mRNAs through alternative splicing . Alternative splicing increases the number of proteins encoded by the genome , but not all alternative mRNAs produce protein . Instead , some are degraded by nonsense-mediated mRNA decay ( NMD ) , a surveillance system that was originally identif...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/post-translational", "regulation", "of", "gene", "expression", "genetics", "and", "genomics/gene", "expression", "computational", "biology/alternative", "splicing", "molecular", "biology/mrna", "stability", "molecular", "biology/rna", "splicing", "genetics...
2009
Genome-Wide Identification of Alternative Splice Forms Down-Regulated by Nonsense-Mediated mRNA Decay in Drosophila
Buruli ulcer [BU] is a chronic and debilitating neglected tropical skin disease caused by Mycobacterium ulcerans . The treatment of moderate to severe BU affects the well-being of entire households and places a strain on both gender relations within households and social relations with kin asked for various types of su...
In this gender-focused study of the neglected tropical disease Buruli ulcer ( BU ) in Benin , West Africa , we document how seeking care for BU is influenced by broad-based concerns about the household production of health and the availability of resources women can mobilize from their social support networks . Women a...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2019
The gendered impact of Buruli ulcer on the household production of health and social support networks: Why decentralization favors women
Long after a new language has been learned and forgotten , relearning a few words seems to trigger the recall of other words . This “free-lunch learning” ( FLL ) effect has been demonstrated both in humans and in neural network models . Specifically , previous work proved that linear networks that learn a set of associ...
If you learn a skill , then partially forget it , does relearning part of that skill induce recovery of other parts of the skill ? More generally , if you learn a set of associations , then partially forget them , does relearning a subset induce recovery of the remaining associations ? In previous work , in which parti...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/evolutionary", "modeling", "computational", "biology/computational", "neuroscience" ]
2008
Falling towards Forgetfulness: Synaptic Decay Prevents Spontaneous Recovery of Memory
The genus Paracoccidioides comprises human thermal dimorphic fungi , which cause paracoccidioidomycosis ( PCM ) , an important mycosis in Latin America . Adaptation to environmental conditions is key to fungal survival during human host infection . The adaptability of carbon metabolism is a vital fitness attribute duri...
The species of the Paracoccidioides genus , a neglected human pathogen , represent the causative agents of paracoccidioidomycosis ( PCM ) , one of the most frequent systemic mycoses in Latin America . Despite being phagocytosed , the fungus conidia differentiate into the parasitic yeast form that subverts the normally ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biochemistry", "cell", "biology", "biology", "and", "life", "sciences", "proteomics", "microbiology", "molecular", "cell", "biology" ]
2014
Transcriptional and Proteomic Responses to Carbon Starvation in Paracoccidioides
Recent heritability analyses have indicated that genome-wide association studies ( GWAS ) have the potential to improve genetic risk prediction for complex diseases based on polygenic risk score ( PRS ) , a simple modelling technique that can be implemented using summary-level data from the discovery samples . We herei...
Large GWAS have identified tens or even hundreds of common SNPs significantly associated with individual complex diseases; however , these SNPs typically explain a small proportion of phenotypic variance . Recently , heritability analyses based on GWAS data suggest that common SNPs have the potential to explain substan...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genome-wide", "association", "studies", "urology", "medicine", "and", "health", "sciences", "body", "fluids", "sociology", "cancers", "and", "neoplasms", "dna-binding", "proteins", "genitourinary", "tract", "tumors", "social", "sciences", "oncology", "bladder", "cancer...
2016
Winner's Curse Correction and Variable Thresholding Improve Performance of Polygenic Risk Modeling Based on Genome-Wide Association Study Summary-Level Data
Oral transmission of Chagas disease has been documented in Latin American countries . Nevertheless , significant studies on the pathophysiology of this form of infection are largely lacking . The few studies investigating oral route infection disregard that inoculation in the oral cavity ( Oral infection , OI ) or by g...
Chagas disease caused by the protozoan Trypanosoma cruzi is endemic in Latin America and a neglected tropical disease , which affects 6–7 million people worldwide . Currently , oral transmission is the most frequent pathway of infection in Brazil but also occurs in other endemic countries . This important infection rou...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?
The influenza A viruses genome comprises eight single-stranded RNA segments of negative polarity . Each one is included in a ribonucleoprotein particle ( vRNP ) containing the polymerase complex and a number of nucleoprotein ( NP ) monomers . Viral RNA replication proceeds by formation of a complementary RNP of positiv...
The influenza A viruses produce annual epidemics and occasional pandemics of respiratory disease . There is great concern about a potential new pandemic being caused by presently circulating avian influenza viruses , and hence increasing interest in understanding how the virus replicates its genome . This comprises eig...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "molecular", "biology/rna-protein", "interactions", "virology/viral", "replication", "and", "gene", "regulation", "infectious", "diseases/respiratory", "infections" ]
2009
Genetic trans-Complementation Establishes a New Model for Influenza Virus RNA Transcription and Replication
Albinism is a genetic defect characterized by a loss of pigmentation . The neurosensory retina , which is not pigmented , exhibits pathologic changes secondary to the loss of pigmentation in the retina pigment epithelium ( RPE ) . How the loss of pigmentation in the RPE causes developmental defects in the adjacent neur...
Albinism is the loss of pigmentation caused by mutations in one of several different genes that alter pigment synthesis by different mechanisms . In the eye , albinism impairs sensory retina development and causes significant vision problems . Regardless of the genetic mutation that causes albinism , the associated vis...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "developmental", "biology", "cell", "biology", "ophthalmology" ]
2008
L-DOPA Is an Endogenous Ligand for OA1
Cryptococcus neoformans is a facultative intracellular pathogen and its interaction with macrophages is a key event determining the outcome of infection . Urease is a major virulence factor in C . neoformans but its role during macrophage interaction has not been characterized . Consequently , we analyzed the effect of...
Cryptococcus neoformans is a relatively frequent cause of life-threatening infection in severely immunocompromised patients , especially those with AIDS . Persistence of infection involves residence within macrophages , where C . neoformans can survive and replicate while residing in the phagolysosome . New treatments ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "cryptococcus", "neoformans", "medicine", "and", "health", "sciences", "urea", "cryptococcus", "immune", "cells", "pathology", "and", "laboratory", "medicine", "chemical", "compounds", "ureases", "yeast", "infections", "enzymes", "pathogens", "immunolo...
2018
Cryptococcus neoformans urease affects the outcome of intracellular pathogenesis by modulating phagolysosomal pH
Human rabies is a significant public health concern in mainland China . However , the neglect of rabies expansion and scarce analyses of the dynamics have made the spatiotemporal spread pattern of human rabies and its determinants being poorly understood . We collected geographic locations and timeline of reported huma...
Although the number of human rabies cases has slightly decreased since 2008 in mainland China , the rabies seemed to be gradually expanding to the low-incidence or non-epidemic areas . The neglect of rabies expansion and scarce analyses of the dynamics have made the spatiotemporal spread pattern of human rabies and its...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
The Spatiotemporal Expansion of Human Rabies and Its Probable Explanation in Mainland China, 2004-2013
Group VI Ca2+-independent phospholipase A2 ( iPLA2 ) is a water-soluble enzyme that is active when associated with phospholipid membranes . Despite its clear pharmaceutical relevance , no X-ray or NMR structural information is currently available for the iPLA2 or its membrane complex . In this paper , we combine homolo...
The Ca2+-independent phospholipase A2 ( iPLA2 ) enzyme is a potential target for the development of medicinal agents against heart and neurological diseases , multiple sclerosis , arthritis , and cancer . However , no structural information is currently available for the iPLA2 . The binding of the enzyme to human membr...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "physics", "biochemistry", "enzyme", "structure", "computational", "chemistry", "molecular", "dynamics", "biophysic", "al", "simulations", "enzyme", "regulation", "enzymes", "macromolecular", "assemblies", "chemistry", "theoretical", "chemistry", "biology", "computational", ...
2013
Insertion of the Ca2+-Independent Phospholipase A2 into a Phospholipid Bilayer via Coarse-Grained and Atomistic Molecular Dynamics Simulations
Horses belong to the order Perissodactyla and bear the majority of their weight on their third toe; therefore , tremendous force is applied to each hoof . An inherited disease characterized by a phenotype restricted to the dorsal hoof wall was identified in the Connemara pony . Hoof wall separation disease ( HWSD ) man...
Inherited diseases affecting only the nails in humans are rare; however , humans do not support themselves entirely on one appendage . Horses bear their entire weight on their third toe , resulting in a large amount of force on each hoof . An inherited disease characterized by a phenotype restricted to separation and b...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
SERPINB11 Frameshift Variant Associated with Novel Hoof Specific Phenotype in Connemara Ponies
Yersinia pestis is the causative agent of human plague and is endemic in various African , Asian and American countries . In Madagascar , the disease represents a significant public health problem with hundreds of human cases a year . Unfortunately , poor infrastructure makes outbreak investigations challenging . DNA w...
Yersinia pestis is a highly pathogenic bacterium and the causative agent of human plague . It has caused three recognized pandemics and is a current human health problem in many countries of Africa , Asia and the Americas , including Madagascar . The pathogen cannot be eradicated from natural plague foci as it persists...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusions" ]
[]
2015
Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007
We describe a new syndrome of young onset diabetes , short stature and microcephaly with intellectual disability in a large consanguineous family with three affected children . Linkage analysis and whole exome sequencing were used to identify the causal nonsense mutation , which changed an arginine codon into a stop at...
The inherited predisposition to type 2 diabetes is attributed to common variants in over 60 loci . Among these risk variants is CDKAL1 , which has recently been shown to be a tRNA modifying enzyme ( methylthiotransferase ) . Genetic variants of different severity can generate a spectrum of monogenic and polygenic forms...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
tRNA Methyltransferase Homolog Gene TRMT10A Mutation in Young Onset Diabetes and Primary Microcephaly in Humans
Helminth infections are prevalent in rural areas of developing countries and have in some studies been negatively associated with allergic disorders and atopy . In this context little is known of the molecular mechanisms of modulation involved . We have characterized the innate immune responses , at the molecular level...
Inflammatory diseases such as atopic disorders are a major health problem in the Western world , but their prevalence is also increasing in developing countries , especially in urban centres . There is increasing evidence that exposure to a rural environment with high burden of compounds derived from parasites and micr...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "immunology/immunomodulation", "immunology/leukocyte", "signaling", "and", "gene", "expression", "immunology/allergy", "and", "hypersensitivity", "infectious", "diseases/helminth", "infections" ]
2008
Lower Expression of TLR2 and SOCS-3 Is Associated with Schistosoma haematobium Infection and with Lower Risk for Allergic Reactivity in Children Living in a Rural Area in Ghana
Despite the success of genome-wide association studies in medical genetics , the underlying genetics of many complex diseases remains enigmatic . One plausible reason for this could be the failure to account for the presence of genetic interactions in current analyses . Exhaustive investigations of interactions are typ...
Many of our common diseases are driven by complex interactions between multiple genetic factors . Disease-specific , genome-wide association studies have been the prominent tool for cataloging such factors , by studying the genetic variation of a gene in a population and its association with the disease . However , the...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Discovering Genetic Interactions in Large-Scale Association Studies by Stage-wise Likelihood Ratio Tests
Organisms have to continuously adapt to changing environmental conditions or undergo developmental transitions . To meet the accompanying change in metabolic demands , the molecular mechanisms of adaptation involve concerted interactions which ultimately induce a modification of the metabolic state , which is character...
Insight into the functioning of metabolic control to meet changing demands is a first step in rational engineering of biological systems towards a desired behavior . Metabolic control analysis provides the means to examine the impact of change of reaction fluxes on a specific target flux based on kinetic modeling , but...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "complex", "systems", "systems", "biology", "game", "theory", "mathematics", "theoretical", "biology", "applied", "mathematics", "biology", "computational", "biology" ]
2013
Structural Control of Metabolic Flux
We describe the development and evaluation of a novel method for targeted amplification and Next Generation Sequencing ( NGS ) -based identification of viral hemorrhagic fever ( VHF ) agents and assess the feasibility of this approach in diagnostics . An ultrahigh-multiplex panel was designed with primers to amplify al...
Viral hemorrhagic fever is a severe and potentially lethal disease , characterized by fever , malaise , vomiting , mucosal and gastrointestinal bleeding , and hypotension , in which multiple organ systems are affected . Due to modern transportation and global trade , outbreaks of viral hemorrhagic fevers have the poten...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "sequencing", "techniques", "medicine", "and", "health", "sciences", "rift", "valley", "fever", "virus", "pathology", "and", "laboratory", "medicine", "togaviruses", "pathogens", "tropical", "diseases", "microbiology", "alphaviruses", "viruses", "next-generation", "sequen...
2017
Development and preliminary evaluation of a multiplexed amplification and next generation sequencing method for viral hemorrhagic fever diagnostics
HIV-1 particles assemble and bud from the plasma membrane of infected T lymphocytes . Infected macrophages , in contrast , accumulate particles within an apparent intracellular compartment known as the virus-containing compartment or VCC . Many aspects of the formation and function of the VCC remain unclear . Here we d...
T lymphocytes and macrophages are the two major cell types involved in HIV replication and transmission events . When a T cell is infected , virus particles assemble and bud from the plasma membrane of the cell . In contrast , infected macrophages develop an intracellular collection of viruses termed the virus-containi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "immunology", "microbiology", "viral", "structure", "retroviruses", "viruses", "immunodeficiency", "viruses", "...
2017
Siglec-1 initiates formation of the virus-containing compartment and enhances macrophage-to-T cell transmission of HIV-1
Meiotic recombination is initiated by large numbers of developmentally programmed DNA double-strand breaks ( DSBs ) , ranging from dozens to hundreds per cell depending on the organism . DSBs formed in single-copy sequences provoke recombination between allelic positions on homologous chromosomes , but DSBs can also fo...
Meiosis is the cell division that generates gametes for sexual reproduction . During meiosis , homologous recombination occurs frequently , initiated by DNA double-strand breaks ( DSBs ) made by Spo11 . Meiotic recombination usually occurs between sequences at allelic positions on homologous chromosomes , but a DSB wit...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Meiotic Recombination Initiation in and around Retrotransposable Elements in Saccharomyces cerevisiae
Tumorigenesis requires the re-organization of metabolism to support malignant proliferation . We examine how the altered metabolism of cancer cells is reflected in the rewiring of co-expression patterns among metabolic genes . Focusing on breast and clear-cell kidney tumors , we report the existence of key metabolic ge...
The metabolism of malignant tumors is deranged . The transition from healthy to cancerous state involves , among other factors , the transcriptional coordination of genes spread throughout the cell’s metabolic pathways . An examination of this multivariate regulatory effort can offer insights which may remain hidden fr...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Extensive Decoupling of Metabolic Genes in Cancer
Multiple GWAS studies have reported strong association of cardiac QT-interval to a region on HSA17 . Interestingly , a rat locus homologous to this region is also linked to QT-intervals . The high resolution positional mapping study located the rat QT-interval locus to a <42 . 5kb region on RNO10 . This region containe...
Diseases of the cardiovascular system such as essential hypertension do not have a clear cause , but are known to run in families . The inheritance patterns of essential hypertension and other cardiac diseases suggest that they are not due to a single defective gene but instead are caused by multiple genetic defects th...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "quantitative", "trait", "loci", "diet", "long", "non-coding", "rnas", "electrocardiography", "nutrition", "bioassays", "and", "physiological", "analysis", "molecular", "biology", "techniques", "research", "and", "analysis", "met...
2017
Positional cloning of quantitative trait nucleotides for blood pressure and cardiac QT-interval by targeted CRISPR/Cas9 editing of a novel long non-coding RNA
Introduced transinfections of the inherited bacteria Wolbachia can inhibit transmission of viruses by Aedes mosquitoes , and in Ae . aegypti are now being deployed for dengue control in a number of countries . Only three Wolbachia strains from the large number that exist in nature have to date been introduced and chara...
Mosquito-borne viral diseases represent an increasing threat to human and animal health globally . The mosquito species Aedes aegypti , a primary vector of the most significant human arboviral infections including the dengue , Zika and Chikungunya viruses , is highly invasive and is almost ubiquitous in tropical urban ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "dengue", "virus", "medicine", "and", "health", "sciences", "reproductive", "system", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "animals", "wolbachia", "viruses", "developmental", "biology", "rna", "viruses", "insect", "...
2018
The Wolbachia strain wAu provides highly efficient virus transmission blocking in Aedes aegypti
Prion proteins can adopt self-propagating alternative conformations that account for the infectious nature of transmissible spongiform encephalopathies ( TSEs ) and the epigenetic inheritance of certain traits in yeast . Recent evidence suggests a similar propagation of misfolded proteins in the spreading of pathology ...
Alzheimer's , Parkinson's , Huntington's , frontotemporal lobar degeneration ( FTLD ) , amyotrophic lateral sclerosis ( ALS ) , and prion diseases are all age-related , fatal neurodegenerative disorders . Hallmarks of these diseases include the expression of toxic protein species . The ability to spread and infect naiv...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "biochemistry", "infectious", "diseases", "model", "organisms", "neurological", "disorders", "neurology", "genetics", "biology", "molecular", "cell", "biology", "neuroscience", "genetics", "and", "genomics" ]
2013
Spreading of a Prion Domain from Cell-to-Cell by Vesicular Transport in Caenorhabditis elegans
Neuritogenesis is a critical early step in the development and maturation of neurons and neuronal circuits . While extracellular directional cues are known to specify the site and orientation of nascent neurite formation in vivo , little is known about the genetic pathways that block inappropriate neurite emergence in ...
Neurons are among the most morphologically complex cells in the body . Early in development , newly born neurons project one or more processes called neurites that will eventually mature into axons and dendrites . While the genetic determinants that promote neurite emergence along specific trajectories are beginning to...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "model", "organisms", "genetics", "biology", "neuroscience", "genetics", "and", "genomics" ]
2011
VANG-1 and PRKL-1 Cooperate to Negatively Regulate Neurite Formation in Caenorhabditis elegans
Genome-wide association studies ( GWAS ) have generated sufficient data to assess the role of selection in shaping allelic diversity of disease-associated SNPs . Negative selection against disease risk variants is expected to reduce their frequencies making them overrepresented in the group of minor ( <50% ) alleles . ...
We reviewed several thousand genome wide association studies that were conducted to identify genetic variants influencing risk of human diseases . We tested the hypothesis that single nucleotide polymorphisms ( SNPs ) that influence disease risk undergo positive or negative selection more frequently than an average SNP...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Allelic Spectra of Risk SNPs Are Different for Environment/Lifestyle Dependent versus Independent Diseases
Identifying the genes that influence levels of pro-inflammatory molecules can help to elucidate the mechanisms underlying this process . We first conducted a two-stage genome-wide association scan ( GWAS ) for the key inflammatory biomarkers Interleukin-6 ( IL-6 ) , the general measure of inflammation erythrocyte sedim...
Inflammation is a protective response of our organism to harmful stimuli—such as germs , damaged cells , or irritants—and to initiate the healing process . It has also been implicated , with both protective and predisposing effects , in a number of different diseases; but many important details of this complex phenomen...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "mathematics", "statistics", "genetics", "biology", "genetics", "and", "genomics" ]
2012
A Genome-Wide Association Scan on the Levels of Markers of Inflammation in Sardinians Reveals Associations That Underpin Its Complex Regulation
Although host genetics influences susceptibility to tuberculosis ( TB ) , few genes determining disease outcome have been identified . We hypothesized that macrophages from individuals with different clinical manifestations of Mycobacterium tuberculosis ( Mtb ) infection would have distinct gene expression profiles and...
Although TB is a leading cause of death worldwide , the vast majority of infected individuals are asymptomatic and contains the bacillus in a latent form . Among those with active disease , 80% have localized pulmonary disease and 20% have disseminated forms . TB meningitis ( TBM ) is the most severe form of TB with 20...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/infectious", "diseases", "of", "the", "nervous", "system", "respiratory", "medicine/respiratory", "infections", "genetics", "and", "genomics/gene", "expression", "genetics", "and", "genomics/genetics", "of", "disease", "infectious", "diseases/bacterial...
2008
Identification of Tuberculosis Susceptibility Genes with Human Macrophage Gene Expression Profiles
The theoretical setting of hierarchical Bayesian inference is gaining acceptance as a framework for understanding cortical computation . In this paper , we describe how Bayesian belief propagation in a spatio-temporal hierarchical model , called Hierarchical Temporal Memory ( HTM ) , can lead to a mathematical model fo...
Understanding the computational and information processing roles of cortical circuitry is one of the outstanding problems in neuroscience . In this paper , we work from a theory of neocortex that models it as a spatio-temporal hierarchical system to derive a biological cortical circuit . This is achieved by combining t...
[ "Abstract", "Introduction", "Model", "Results", "Discussion" ]
[ "neuroscience/natural", "and", "synthetic", "vision", "neuroscience/theoretical", "neuroscience" ]
2009
Towards a Mathematical Theory of Cortical Micro-circuits
The rhoptry of the malaria parasite Plasmodium falciparum is an unusual secretory organelle that is thought to be related to secretory lysosomes in higher eukaryotes . Rhoptries contain an extensive collection of proteins that participate in host cell invasion and in the formation of the parasitophorous vacuole , but l...
The malaria parasite Plasmodium falciparum is a eukaryotic organism with multiple membrane bound organelles with discrete functions . The rhoptry is an unusual secretory organelle that participates in host cell invasion and the formation of a specialised vacuole that the parasite occupies during the intracellular part ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology/membranes", "and", "sorting", "infectious", "diseases/protozoal", "infections" ]
2009
Identification of Rhoptry Trafficking Determinants and Evidence for a Novel Sorting Mechanism in the Malaria Parasite Plasmodium falciparum
To investigate the DDT and deltamethrin susceptibility of Phlebotomus argentipes , the vector of Leishmania donovani , responsible for visceral leishmaniasis ( VL ) , in two countries ( India and Nepal ) with different histories of insecticide exposure . Standard WHO testing procedures were applied using 4% DDT and 0 ....
Visceral leishmaniasis ( VL ) , also know as kala azar , is one of the major public health concerns India , Nepal and Bangladesh . In the Indian subcontinent , VL is caused by Leishmania donovani which is transmitted by Phlebotomus argentipes . To date , Indoor Residual Spraying ( IRS ) campaigns have been unable to co...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "public", "health", "and", "epidemiology/infectious", "diseases" ]
2010
Insecticide Susceptibility of Phlebotomus argentipes in Visceral Leishmaniasis Endemic Districts in India and Nepal
What is the underlying mechanism behind the fat-tailed statistics observed for species abundance distributions ? The two main hypotheses in the field are the adaptive ( niche ) theories , where species abundance reflects its fitness , and the neutral theory that assumes demographic stochasticity as the main factor dete...
One purchases 100 wineglasses and 100 pairs of pants . After one year , 10 glasses and 10 pants survive . What can be said about the relative quality of the survivors ? Well , clothes “die” as a result of accumulated wear; the surviving items are of better quality . The breaking of a wineglass is an external , random e...
[ "Abstract", "Introduction", "Results/Discussion", "Methods" ]
[ "ecology/theoretical", "ecology", "ecology/population", "ecology", "ecology/evolutionary", "ecology", "genetics", "and", "genomics/population", "genetics" ]
2009
Polymorphism Data Can Reveal the Origin of Species Abundance Statistics
During sensory deprivation , the barrel cortex undergoes expansion of a functional column representing spared inputs ( spared column ) , into the neighboring deprived columns ( representing deprived inputs ) which are in turn shrunk . As a result , the neurons in a deprived column simultaneously increase and decrease t...
Plasticity in the adult neocortex is the basis of our learning and memory . However , its molecular mechanisms are still unclear . In the sensory barrel cortex of rodents , a well-characterized model for neocortical plasticity , neurons directly code for whisker displacement—neurons within a given barrel will fire when...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Cofilin1 Controls Transcolumnar Plasticity in Dendritic Spines in Adult Barrel Cortex
Ectopic heartbeats can trigger reentrant arrhythmias , leading to ventricular fibrillation and sudden cardiac death . Such events have been attributed to perturbed Ca2+ handling in cardiac myocytes leading to spontaneous Ca2+ release and delayed afterdepolarizations ( DADs ) . However , the ways in which perturbation o...
Arrhythmias are electrical abnormalities of the heart that can degenerate into fibrillation , thus preventing normal heartbeats and leading to sudden cardiac death . The mechanisms leading to ventricular arrhythmias and the unexpected nature of sudden cardiac death are not fully understood . One hypothesis is that a gr...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "cell", "physiology", "medicine", "and", "health", "sciences", "action", "potentials", "muscle", "tissue", "nervous", "system", "membrane", "potential", "condensed", "matter", "physics", "junctional", "complexes", "electrophysiology", "neuroscience", "simulation", "and", ...
2017
Estimating the probabilities of rare arrhythmic events in multiscale computational models of cardiac cells and tissue
An understanding of the factors driving the distribution of pathogens is useful in preventing disease . Often we achieve this understanding at a local microhabitat scale; however the larger scale processes are often neglected . This can result in misleading inferences about the distribution of the pathogen , inhibiting...
Many pathogens persist in the environment , and an understanding of where they are can assist in disease control , allowing us to identify areas of risk to local human populations . Herein , we use general linear models to describe the distribution of a particular environmental pathogen , Mycobacterium ulcerans , descr...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "ecology", "and", "environmental", "sciences", "medicine", "and", "health", "sciences", "ecological", "niches", "ecology", "neglected", "tropical", "diseases", "biology", "and", "life", "sciences", "tropical", "diseases" ]
2014
Topography and Land Cover of Watersheds Predicts the Distribution of the Environmental Pathogen Mycobacterium ulcerans in Aquatic Insects
The phosphoinositide-3 kinase ( PI3K ) pathway regulates diverse cellular activities related to cell growth , migration , survival , and vesicular trafficking . It is known that Ebola virus requires endocytosis to establish an infection . However , the cellular signals that mediate this uptake were unknown for Ebola vi...
Each year , filoviruses such as Ebola virus claim many human lives and decimate gorilla populations in Africa . Infection results in an acute fever often associated with profuse internal and external bleeding and death rates of up to 90% . Due to these symptoms and high pathogenicity , these viruses have been heavily p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/animal", "models", "of", "infection", "virology/antivirals,", "including", "modes", "of", "action", "and", "resistance", "virology", "virology/effects", "of", "virus", "infection", "on", "host", "gene", "expression", "virology/host", "invasion", "and", "cell",...
2008
Phosphoinositide-3 Kinase-Akt Pathway Controls Cellular Entry of Ebola Virus
Although plasma leakage is the hallmark of severe dengue infections , the factors that cause increased vascular permeability have not been identified . As platelet activating factor ( PAF ) is associated with an increase in vascular permeability in other diseases , we set out to investigate its role in acute dengue inf...
Although plasma leakage is the hallmark of severe dengue infections , the factors that cause increased vascular permeability have not been identified . As platelet activating factor ( PAF ) is associated with an increase in vascular permeability in other diseases , we set out to investigate its role in acute dengue inf...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Platelet Activating Factor Contributes to Vascular Leak in Acute Dengue Infection
Over time , a population acquires neutral genetic substitutions as a consequence of random drift . A famous result in population genetics asserts that the rate , K , at which these substitutions accumulate in the population coincides with the mutation rate , u , at which they arise in individuals: K = u . This identity...
Evolution is driven by genetic mutations . While some mutations affect an organism’s ability to survive and reproduce , most are neutral and have no effect . Neutral mutations play an important role in the study of evolution because they generally accrue at a consistent rate over time . This result , first discovered 5...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
The Molecular Clock of Neutral Evolution Can Be Accelerated or Slowed by Asymmetric Spatial Structure
Fatal Ebola virus infection is characterized by a systemic inflammatory response similar to septic shock . Ebola glycoprotein ( GP ) is involved in this process through activating dendritic cells ( DCs ) and macrophages . However , the mechanism is unclear . Here , we showed that LSECtin ( also known as CLEC4G ) plays ...
Ebola virus ( EBOV ) , a highly virulent pathogen , causes a severe hemorrhagic fever syndrome . The fatal infection is characterized by a systemic inflammatory response similar to septic shock . Ebola glycoprotein ( GP ) is thought to contribute to disease pathogenesis , as high amounts of shed GP from virus-infected ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "phosphorylation", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "physiology", "cytokines", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "molecular", "probe", "techniques", "gene", "regulation", "pathoge...
2016
The Myeloid LSECtin Is a DAP12-Coupled Receptor That Is Crucial for Inflammatory Response Induced by Ebola Virus Glycoprotein
Clonorchis sinensis ( C . sinensis ) is considered to be an important parasitic zoonosis because it infects approximately 35 million people , while approximately 15 million were distributed in China . Hepatitis B virus ( HBV ) infection is a major public health issue . Two types of pathogens have the potential to cause...
Clonorchiasis and hepatitis B infection are infectious diseases that affect millions of people worldwide , especially in China . These two diseases are caused by two different pathogens , C . sinensis and hepatitis B virus , respectively . Concurrent infection between HBV and C . sinensis is often observed in some area...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "physiology", "cytokines", "pathology", "and", "laboratory", "medicine", "china", "antiviral", "therapy", "pathogens", "immunology", "tropical", "diseases", "microbiology", "geographical", ...
2016
Clonorchis sinensis Co-infection Could Affect the Disease State and Treatment Response of HBV Patients
Information on crop pedigrees can be used to help maximise genetic gain in crop breeding and allow efficient management of genetic resources . We present a pedigree resource of 2 , 657 wheat ( Triticum aestivum L . ) genotypes originating from 38 countries , representing more than a century of breeding and variety deve...
Breeding activities undertaken in the world’s most important crop species have resulted in large increases in yield potential over the last century . Bread wheat is a key crop for both human and animal nutrition worldwide . To help inform future breeding and research activities , we have developed a pedigree resource o...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "ecology", "and", "environmental", "sciences", "variant", "genotypes", "crop", "genetics", "genetic", "mapping", "cereal", "crops", "plant", "science", "crops", "molecular", "biology", "techniques", "molecular", "genetics", "plants", "research", "and", "analysis", "me...
2019
A large-scale pedigree resource of wheat reveals evidence for adaptation and selection by breeders
The C-type lectin DC-SIGN ( CD209 ) is known to be the major dengue receptor on human dendritic cells , and a single nucleotide polymorphism ( SNP ) in the promoter region of CD209 ( −336 A/G; rs4804803 ) is susceptible to many infectious diseases . We reason that variations in the DC-SIGN gene might have a broad influ...
Dengue fever ( DF ) is an arthropod-borne disease that is prevalent in tropical and subtropical regions of the world . DC-SIGN [dendritic cell-specific intercellular adhesion molecule 3 ( ICAM-3 ) -grabbing non-integrin] is a major receptor for dengue infection . DC-SIGN , also called CD209 , expresses on dendritic cel...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "genetics", "and", "genomics/genetics", "of", "the", "immune", "system", "immunology/genetics", "of", "the", "immune", "system", "infectious", "diseases/viral", "infections", "infectious", "diseases/tropical", "and", "travel-associated", "diseases", "immunology/immunity", "...
2011
DC-SIGN (CD209) Promoter −336 A/G Polymorphism Is Associated with Dengue Hemorrhagic Fever and Correlated to DC-SIGN Expression and Immune Augmentation
Mouse sex determination provides an attractive model to study how regulatory genetic networks and signaling pathways control cell specification and cell fate decisions . This study characterizes in detail the essential role played by the insulin receptor ( INSR ) and the IGF type I receptor ( IGF1R ) in adrenogenital d...
Congenital disorders of sexual differentiation are rare diseases in which there is discordance between chromosomal , gonadal , and phenotypic sex . Unfortunately , only a minority of patients clinically diagnosed with disorders of sex development ( DSD ) obtains a molecular diagnosis , indicating that our understanding...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "cell", "fate", "determination", "functional", "genomics", "gene", "expression", "genetics", "biology", "genomics", "genetics", "and", "genomics", "cell", "differentiation", "dna", "transcription", "gene", "function" ]
2013
Insulin and IGF1 Receptors Are Essential for XX and XY Gonadal Differentiation and Adrenal Development in Mice
The orderly packing and precise arrangement of epithelial cells is essential to the functioning of many tissues , and refinement of this packing during development is a central theme in animal morphogenesis . The mechanisms that determine epithelial cell shape and position , however , remain incompletely understood . H...
Many tissues and organs , including sensory organs like the vertebrate retina and inner ear , are built from sheets of connected cells called epithelia . The precise arrangement of different types of cells within these epithelia can be essential to their function . ( For example , photoreceptor cells in eyes must be pr...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "statistical", "mechanics", "neuroscience", "biomechanics", "animal", "models", "developmental", "biology", "model", "organisms", "cell", "mechanics", "morphogenesis", "pattern", "formation", "developmental", "neuroscience", "theoretical", "biology", "biophysics", "theory", ...
2012
Coupling Mechanical Deformations and Planar Cell Polarity to Create Regular Patterns in the Zebrafish Retina
Zika virus infection is associated with the development of Guillain-Barré syndrome ( GBS ) , a neurological autoimmune disorder caused by immune recognition of gangliosides and other components at nerve membranes . Using a high-throughput ELISA , we have analyzed the anti-glycolipid antibody profile , including ganglio...
Zika virus infection can trigger the development of Guillain Barré syndrome ( GBS ) , a neurological autoimmune disorder mediated by antibodies recognizing gangliosides in nerve membranes . Mechanisms such as molecular mimicry have been identified as a cause for GBS development in certain infections , such as Campyloba...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "glycolipids", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "chikungunya", "infection", "sphingolipids", "pathogens", "immunology", "tropical", "diseases", "microbiology", "vi...
2019
Anti-ganglioside antibodies in patients with Zika virus infection-associated Guillain-Barré Syndrome in Brazil
Morphogenetic gradients are essential to allocate cell fates in embryos of varying sizes within and across closely related species . We previously showed that the maternal NF-κB/Dorsal ( Dl ) gradient has acquired different shapes in Drosophila species , which result in unequally scaled germ layers along the dorso-vent...
Embryo size can vary greatly among closely related species . How tissue specification either scales or is modified in the developing embryo in different species is an ongoing investigation in developmental biology . Here we asked how embryo morphology and specific molecular pathways influence tissue specification by al...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "morphogens", "developmental", "biology", "molecular", "development", "biology", "and", "life", "sciences", "cell", "fate", "determination", "evolutionary", "developmental", "biology" ]
2014
Modeling of the Dorsal Gradient across Species Reveals Interaction between Embryo Morphology and Toll Signaling Pathway during Evolution
Rift Valley fever ( RVF ) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula . A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures . A first-line l...
Rift Valley fever ( RVF ) is a viral disease that affects a wide range of animals and human beings in Africa and the Arabian Peninsula involving low case fatality rates . A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures . Here , we rep...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Discussion" ]
[ "medicine", "and", "health", "sciences", "rift", "valley", "fever", "virus", "enzyme-linked", "immunoassays", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "tropical", "diseases", "microbiology", "viruses", "rift", "val...
2019
Development and validation of a pen side test for Rift Valley fever
Engineered synthetic biological devices have been designed to perform a variety of functions from sensing molecules and bioremediation to energy production and biomedicine . Notwithstanding , a major limitation of in vivo circuit implementation is the constraint associated to the use of standard methodologies for circu...
Synthetic biological circuits have been built for different purposes . Nevertheless , the way these devices have been designed so far present several limitations: complex genetic engineering is required to implement complex circuits , and once the parts are built , they are not reusable . We proposed to distribute the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "engineering", "and", "technology", "pathogens", "sociology", "microbiology", "social", "sciences", "light", "microscopy", "logic", "circuits", "fungi", "model", "org...
2016
Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia