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Glycans play important roles in host-microbe interactions . Tissue-specific expression patterns of the blood group glycosyltransferase β-1 , 4-N-acetylgalactosaminyltransferase 2 ( B4galnt2 ) are variable in wild mouse populations , and loss of B4galnt2 expression is associated with altered intestinal microbiota . We h...
Human blood groups are among the oldest known genetic polymorphisms . It has been proposed that blood group variation is a byproduct of pathogen-driven selection , including in the gastrointestinal tract where blood-group-related genes are often variably expressed . The B4galnt2 gene is responsible for the synthesis of...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "Methods" ]
[]
2015
Expression of the Blood-Group-Related Gene B4galnt2 Alters Susceptibility to Salmonella Infection
DNA double-strand breaks ( DSB ) are very harmful lesions that can generate genome rearrangements . In this study , we used intrachromosomal reporters to compare both the efficiency and accuracy of end-joining occurring with close ( 34 bp apart ) vs . distant DSBs ( 3200 bp apart ) in human fibroblasts . We showed that...
A DNA double-strand break is a very toxic lesion that can be repaired by rejoining DNA ends . This repair process can have deleterious consequences on the genome by joining DNA ends that were not originally fused ( translocations ) or modifying the DNA sequence with deletions or insertions . Here , we show that rejoini...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "sequencing", "techniques", "meiosis", "medicine", "and", "health", "sciences", "gene", "regulation", "cell", "cycle", "and", "cell", "division", "cell", "processes", "surgical", "and", "invasive", "medical", "procedures", "dna", "replication", "dna", "molecular", "...
2016
53BP1 Protects against CtIP-Dependent Capture of Ectopic Chromosomal Sequences at the Junction of Distant Double-Strand Breaks
A developing plant organ exhibits complex spatiotemporal patterns of growth , cell division , cell size , cell shape , and organ shape . Explaining these patterns presents a challenge because of their dynamics and cross-correlations , which can make it difficult to disentangle causes from effects . To address these pro...
Organ morphogenesis involves two coordinated processes: growth of tissue and increase in cell number through cell division . Both processes have been analysed individually in many systems and shown to exhibit complex patterns in space and time . However , it is unclear how these patterns of growth and cell division are...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "skin", "cell", "physiology", "plant", "anatomy", "medicine", "and", "health", "sciences", "integumentary", "system", "cell", "division", "analysis", "cell", "cycle", "and", "cell", "division", "cell", "processes", "brassica", "cell", "polarity", "plant", "science",...
2018
Spatiotemporal coordination of cell division and growth during organ morphogenesis
The allosteric mechanism plays a key role in cellular functions of several PDZ domain proteins ( PDZs ) and is directly linked to pharmaceutical applications; however , it is a challenge to elaborate the nature and extent of these allosteric interactions . One solution to this problem is to explore the dynamics of PDZs...
PDZ domain proteins ( PDZs ) act as adapters in organizing functional protein complexes . Through dynamic interactions , PDZs play a key role in mediating key cellular functions in the cell , and they are linked to currently challenging diseases including Alzheimer's , Parkinson's and cancer . Moreover , they are assoc...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "biology", "computational", "biology" ]
2011
Change in Allosteric Network Affects Binding Affinities of PDZ Domains: Analysis through Perturbation Response Scanning
Polyketides , a diverse group of heteropolymers with antibiotic and antitumor properties , are assembled in bacteria by multiprotein chains of modular polyketide synthase ( PKS ) proteins . Specific protein–protein interactions determine the order of proteins within a multiprotein chain , and thereby the order in which...
Biomolecular interactions can be extraordinarily specific . In many instances , a protein can select its single correct binding partner from among a large array of closely related candidates . For polyketide synthases ( PKSs ) , a family of bacterial enzymes , such specificity is essential . Like workers on an assembly...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "evolutionary", "biology", "eubacteria", "computational", "biology" ]
2007
The Origins of Specificity in Polyketide Synthase Protein Interactions
Depression is characterized by a marked decrease in social interactions and blunted sensitivity to rewards . Surprisingly , despite the importance of social deficits in depression , non-social aspects have been disproportionally investigated . As a consequence , the cognitive mechanisms underlying atypical decision-mak...
Blunted sensitivity to rewards is at the core of depression . However , studies that investigated the influence of depression on decision-making have often done so in asocial contexts , thereby providing only partial insights into the way depressive disorders impact the underlying cognitive processes . Indeed , atypica...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "learning", "medicine", "and", "health", "sciences", "decision", "making", "statistics", "metaanalysis", "social", "sciences", "neuroscience", "learning", "and", "memory", "simulation", "and", "modeling", "cognitive", "psychology", "mathematics", "anxiety", "cognition", ...
2019
Depressive symptoms are associated with blunted reward learning in social contexts
The ascending modulatory systems of the brain stem are powerful regulators of global brain state . Disturbances of these systems are implicated in several major neuropsychiatric disorders . Yet , how these systems interact with specific neural computations in the cerebral cortex to shape perception , cognition , and be...
The human brain is equipped with a number of modulatory neurotransmitter systems , which have widespread projections and regulate global brain states . Disturbances of these systems are implicated in several neuropsychiatric disorders , but how they modulate specific neural computations to shape perception , cognition ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "chemical", "compounds", "acetylcholine", "social", "sciences", "neuroscience", "organic", "compounds", "hormones", "mathematics", "brain", "mapping", "amines", "vision", "neurotransmitters", "catecholamines", "disc...
2018
Catecholamines alter the intrinsic variability of cortical population activity and perception
Various types of neural-based signals , such as EEG , local field potentials and intracellular synaptic potentials , integrate multiple sources of activity distributed across large assemblies . They have in common a power-law frequency-scaling structure at high frequencies , but it is still unclear whether this scaling...
Intracellular recording of neocortical neurons provides an opportunity of characterizing the statistical signature of the synaptic bombardment to which it is submitted . Indeed the membrane potential displays intense fluctuations which reflect the cumulative activity of thousands of input neurons . In sensory cortical ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/theoretical", "neuroscience", "neuroscience/sensory", "systems" ]
2009
Network-State Modulation of Power-Law Frequency-Scaling in Visual Cortical Neurons
We recently described a new , live-attenuated vaccine candidate for chikungunya ( CHIK ) fever , CHIKV/IRES . This vaccine was shown to be well attenuated , immunogenic and efficacious in protecting against CHIK virus ( CHIKV ) challenge of mice and nonhuman primates . To further evaluate its preclinical safety , we co...
Chikungunya fever is a reemerging , mosquito-borne viral disease that causes severe , debilitating , and often chronic arthralgia . The virus reemerged from Africa in 2004 and has since caused disease in millions of persons , including in over one million in the Americas since it arrived for the first time in modern sc...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
Extended Preclinical Safety, Efficacy and Stability Testing of a Live-attenuated Chikungunya Vaccine Candidate
The predominant mechanism of drug resistance in African trypanosomes is decreased drug uptake due to loss-of-function mutations in the genes for the transporters that mediate drug import . The role of transporters as determinants of drug susceptibility is well documented from laboratory-selected Trypanosoma brucei muta...
Human African Trypanosomiasis , or sleeping sickness , is a fatal disease restricted to sub-Saharan Africa , caused by Trypanosoma brucei gambiense and T . b . rhodesiense . The treatment relies on chemotherapy exclusively . Drug resistance in T . brucei was investigated mainly in laboratory-selected lines and found to...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2013
Aquaporin 2 Mutations in Trypanosoma brucei gambiense Field Isolates Correlate with Decreased Susceptibility to Pentamidine and Melarsoprol
Tsetse flies transmit trypanosomes that cause nagana in cattle , and sleeping sickness in humans . Therefore , optimising visual baits to control tsetse is an important priority . Tsetse are intercepted at visual baits due to their initial attraction to the bait , and their subsequent contact with it due to landing or ...
Tsetse flies transmit trypanosomes that cause sleeping sickness . Visual baits to attract and kill tsetse are an important method of vector control , and the rational improvement of these baits depends on a mechanistic understanding of tsetse behaviour . Visual baits are often panels of insecticide-treated cloth which ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2015
A Receptor-Based Explanation for Tsetse Fly Catch Distribution between Coloured Cloth Panels and Flanking Nets
Many genetic and physiological treatments that extend lifespan also confer resistance to a variety of stressors , suggesting that cytoprotective mechanisms underpin the regulation of longevity . It has not been established , however , whether the induction of cytoprotective pathways is essential for lifespan extension ...
Many mutations that increase animal lifespan also confer stress tolerance , suggesting that cytoprotective mechanisms underpin the regulation of longevity . It has not been established , however , whether the induction of individual cytoprotective pathways is essential for lifespan extension , or merely correlated . To...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cellular", "stress", "responses", "animal", "genetics", "gene", "regulation", "gene", "function", "animal", "models", "caenorhabditis", "elegans", "model", "organisms", "molecular", "genetics", "gene", "expression", "biology", "molecular", "biology", "signal", "transdu...
2012
Induction of Cytoprotective Pathways Is Central to the Extension of Lifespan Conferred by Multiple Longevity Pathways
Attribution of biological robustness to the specific structural properties of a regulatory network is an important yet unsolved problem in systems biology . It is widely believed that the topological characteristics of a biological control network largely determine its dynamic behavior , yet the actual mechanism is sti...
Living organisms exert very complicated control on the functionality of their components . Such control systems can often operate in a surprisingly robust manner , in spite of constant perturbations from fluctuating internal conditions and a volatile external environment . What feature makes such control mechanisms rob...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "computational", "biology/signaling", "networks", "computer", "science/systems", "and", "control", "theory", "computational", "biology/systems", "biology", "computational", "biology" ]
2009
Identification of a Topological Characteristic Responsible for the Biological Robustness of Regulatory Networks
The threadworm , Strongyloides stercoralis , endemic in tropical and temperate climates , is a neglected tropical disease . Its diagnosis requires specific methods , and accurate information on its geographic distribution and global burden are lacking . We predicted prevalence , using Bayesian geostatistical modeling ,...
Data on the prevalence and distribution of Strongyloides stercoralis ( threadworm ) is scarce in many resource-poor countries . We carried out a cross-sectional study during the dry season among 2 , 396 rural Cambodians of all ages . We used a rigorous diagnostic approach , involving two stool samples per person and tw...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "public", "and", "occupational", "health", "infectious", "diseases", "helminth", "infections", "medicine", "and", "health", "sciences", "primary", "care", "environmental", "epidemiology", "epidemiology", "global", "health", "strongyloidiasis", "neglected", "tropical", "di...
2014
High Prevalence and Spatial Distribution of Strongyloides stercoralis in Rural Cambodia
Formation of spatial gene expression patterns in development depends on transcriptional responses mediated by gene control regions , enhancers . Here , we explore possible responses of enhancers to overlapping gradients of antagonistic transcriptional regulators in the Drosophila embryo . Using quantitative models base...
The early development of the fruit fly embryo depends on an intricate but well-studied gene regulatory network . In fly eggs , maternally deposited gene products—morphogenes—form spatial concentration gradients . The graded distribution of the maternal morphogenes initiates a cascade of gene interactions leading to emb...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "drosophila", "developmental", "biology", "computational", "biology" ]
2007
Enhancer Responses to Similarly Distributed Antagonistic Gradients in Development
MicroRNAs ( miRNAs ) are small regulatory RNAs processed from primary miRNA transcripts , and plant miRNAs play important roles in plant growth , development , and response to infection by microbes . Microbial infections broadly alter miRNA biogenesis , but the underlying mechanisms remain poorly understood . In this s...
MicroRNAs ( miRNAs ) regulate gene expression at the transcriptional or post-transcriptional level and have emerged as key players in regulating plant growth , development and response to biotic and abiotic stresses . Accumulating evidences suggest that miRNAs are pivotal modulators of host–virus interactions , but how...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[]
2017
Rice stripe virus NS3 protein regulates primary miRNA processing through association with the miRNA biogenesis factor OsDRB1 and facilitates virus infection in rice
Snake bite causes greater mortality than most of the other neglected tropical diseases . Snake antivenom , although effective in minimizing mortality in developed countries , is not equally so in developing countries due to its poor availability in remote snake infested areas as , and when , required . An alternative a...
Antivenom , the only effective therapy against snake bite in practice , is successful in controlling mortality in developed countries , but not in developing countries . Unavailability of antivenom at the proper time and place of snake bite in developing countries is a major factor in this account , which results not o...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "clinical", "medicine", "primary", "care", "biology", "and", "life", "sciences", "critical", "care", "and", "emergency", "medicine", "toxicology", "health", "care" ]
2014
Viper and Cobra Venom Neutralization by Alginate Coated Multicomponent Polyvalent Antivenom Administered by the Oral Route
Invasion of erythrocytes by Plasmodium falciparum involves a complex cascade of protein-protein interactions between parasite ligands and host receptors . The reticulocyte binding-like homologue ( PfRh ) protein family is involved in binding to and initiating entry of the invasive merozoite into erythrocytes . An impor...
The malaria parasite invades red blood cells by binding to proteins on the surface of this host cell . A family of proteins called P . falciparum reticulocyte binding-like homologue ( PfRh ) proteins are important for recognition of the red blood cell and activation of the invasion process . An important member of the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2011
An EGF-like Protein Forms a Complex with PfRh5 and Is Required for Invasion of Human Erythrocytes by Plasmodium falciparum
To cause disease , Clostridioides ( Clostridium ) difficile must resist killing by innate immune effectors in the intestine , including the host antimicrobial peptide , cathelicidin ( LL-37 ) . The mechanisms that enable C . difficile to adapt to the intestine in the presence of antimicrobial peptides are unknown . Exp...
C . difficile is a major nosocomial pathogen that causes severe diarrheal disease . Though C . difficile is known to inhabit the human gastrointestinal tract , the mechanisms that allow this pathogen to adapt to the intestine and survive host defenses are not known . In this work , we investigated the response of C . d...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "gut", "bacteria", "toxins", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "operons", "vertebrates", "animals", "mammals", "toxicology", "toxic", "agents", "analysis", "of", "variance", "regulator", "genes...
2018
The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37
The flipping-out of a DNA base from the double helical structure is a key step of many cellular processes , such as DNA replication , modification and repair . Base pair opening is the first step of base flipping and the exact mechanism is still not well understood . We investigate sequence effects on base pair opening...
The DNA double helix , a molecule that stores biological information , has become an iconic image of biomedical research . In order to use or repair the information it carries , the bases that are stacked in the helix need to be chemically exposed . This can happen either by separating the two strands in the helix or b...
[ "Abstract", "Introduction", "Methods", "Results", "and", "discussion" ]
[ "protons", "chemical", "bonding", "molecular", "dynamics", "geometry", "simulation", "and", "modeling", "mathematics", "dna", "dna", "structure", "thermodynamics", "hydrogen", "bonding", "physical", "chemistry", "research", "and", "analysis", "methods", "chemistry", "nu...
2017
Sequence dependency of canonical base pair opening in the DNA double helix
We contribute a new methodological approach to the ongoing efforts towards evaluating public health surveillance . Specifically , we apply a descriptive framework , grounded in prospect theory ( PT ) , for the evaluation of decisions on disease surveillance deployment . We focus on two attributes of any surveillance sy...
In this paper we contribute a new methodological approach to the ongoing efforts towards evaluating public health surveillance . Specifically , we apply a descriptive framework for the evaluation of decisions on disease surveillance deployment . We focus on two attributes of any surveillance system: timeliness and fals...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "decision", "making", "tropical", "diseases", "social", "sciences", "neuroscience", "arithmetic", "research", "design", "rabies", "cognitive", "psychology", "mathematics", "cognition", "neglected", "tropical", "diseases", "question...
2019
Unbiased assessment of disease surveillance utilities: A prospect theory application
Current understanding of adaptive immune , particularly T cell , responses to human rhinoviruses ( RV ) is limited . Memory T cells are thought to be of a primarily T helper 1 type , but both T helper 1 and T helper 2 memory cells have been described , and heightened T helper 2/ lessened T helper 1 responses have been ...
Rhinovirus infections cause the common cold and a high proportion of exacerbations of asthma and chronic obstructive airway disease . Little is understood of the roles of T cells in causing or limiting rhinovirus disease . Rhinovirus-specific memory cells are primarily of a T helper 1 type in healthy people , but rhino...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "t", "helper", "cells", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "respiratory", "infections", "cytokines", "enzyme-linked", "immunoassays", "immunology", "pulmonology", "developme...
2016
Tbet Deficiency Causes T Helper Cell Dependent Airways Eosinophilia and Mucus Hypersecretion in Response to Rhinovirus Infection
We introduce in this paper a new method for reducing neurodynamical data to an effective diffusion equation , either experimentally or using simulations of biophysically detailed models . The dimensionality of the data is first reduced to the first principal component , and then fitted by the stationary solution of a m...
We introduce a novel methodology that allows for an effective description of a neurodynamical system in a data-driven fashion . In particular , no knowledge of the dynamics operating at the neuronal or synaptic level is required . The idea is to fit the underlying dynamics of the data using a stochastic differential eq...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "neuroscience/theoretical", "neuroscience" ]
2009
Effective Reduced Diffusion-Models: A Data Driven Approach to the Analysis of Neuronal Dynamics
Trypanosoma ( T . ) evansi is a dyskinetoplastic variant of T . brucei that has gained the ability to be transmitted by all sorts of biting flies . T . evansi can be divided into type A , which is the most abundant and found in Africa , Asia and Latin America and type B , which has so far been isolated only from Kenyan...
Trypanosoma ( T . ) evansi causes surra in various animal species in Africa , Latin America and Asia . Despite inducing important animal suffering , economic losses and being a World Animal Health Organisation ( OIE ) notifiable disease , surra is severely neglected in terms of awareness , control interventions and res...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "body", "fluids", "vertebrates", "cloning", "parasitic", "diseases", "parasitic", "protozoans", "mammals", "animals", "protozoans", "molecular", "biology", "techniques", "cellular", "structures", "and", "organelles", "extraction", ...
2016
New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels
Mass drug administration ( MDA ) , targeted at school-aged children ( SAC ) is the method recommended by the World Health Organization for the control of morbidity induced by soil-transmitted helminth ( STH ) infection in endemic countries . However , MDA does not prevent reinfection between treatment rounds and resear...
Mass drug administration ( MDA ) , treating either whole communities or targeted groups without a prior diagnosis , is used as a control strategy for many neglected tropical diseases , including soil-transmitted helminth ( STH ) infection . MDA takes place at set intervals , aiming to reduce morbidity caused by the tar...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "helminths", "tropical", "diseases", "hookworms", "geographical", "locations", "parasitic", "diseases", "animals", "health", "care", "age", "groups", "ascaris", "ascaris", "lumbricoides", "myanmar", "neglected", ...
2019
Soil-transmitted helminth reinfection four and six months after mass drug administration: results from the delta region of Myanmar
Interleukin 17 ( IL-17 ) –producing γδ T cells ( γδ17 T cells ) have been recently found to promote tumor growth and metastasis formation . How such γδ17 T-cell responses may be regulated in the tumor microenvironment remains , however , largely unknown . Here , we report that tumor-associated neutrophils can display a...
Tumors are infiltrated by many immune cells that influence many aspects of cancer progression and outcome , including tumor growth , invasion of healthy surrounding tissues , formation of metastasis , and response to treatments . Among tumor-infiltrating lymphocytes , γδ T cells play dual functions in the tumor milieu;...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "reactive", "oxygen", "species", "immune", "cells", "chemical", "compounds", "oxides", "oxidative", "stress", "immunology", "animal", "models", "model", "organisms", "experimental", "organism", "systems", "cyt...
2018
Tumor-associated neutrophils suppress pro-tumoral IL-17+ γδ T cells through induction of oxidative stress
Chromatin-modifying enzymes and ATP-dependent remodeling complexes have been intensely studied individually , yet how these activities are coordinated to ensure essential cell functions such as transcription , replication , and repair of damage is not well understood . In this study , we show that the critical loss of ...
In eukaryotes , essential processes such as transcription , replication , and repair of damage occur in the context of chromatin . The structure of chromatin is tightly regulated during the cell cycle by chromatin-modifying enzymes , including acetyltransferases , and ATP-dependent remodeling complexes . Although there...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "gene", "expression", "genetics", "epigenetics", "biology", "chromatin", "genetics", "and", "genomics", "dna", "transcription", "histone", "modification" ]
2012
Functional Antagonism between Sas3 and Gcn5 Acetyltransferases and ISWI Chromatin Remodelers
Epidemics of meningococcal meningitis ( MM ) recurrently strike the African Meningitis Belt . This study aimed at investigating factors , still poorly understood , that influence annual incidence of MM serogroup A , the main etiologic agent over 2004–2010 , at a fine spatial scale in Niger . To take into account data d...
Meningococcal meningitis ( MM ) is a severe infection of the meninges caused by a bacterium transmitted through respiratory droplets . During January–May , epidemics of MM recurrently strike sub-Saharan countries , including Niger . Understanding why epidemics occur in a particular place at a particular time would help...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "atmospheric", "science", "spatial", "epidemiology", "geoinformatics", "bacterial", "diseases", "mathematics", "statistics", "(mathematics)", "population", "modeling", "spatial", "analysis", "human", "geography", "public", "and", "...
2014
Spatio-Temporal Factors Associated with Meningococcal Meningitis Annual Incidence at the Health Centre Level in Niger, 2004–2010
Leishmania is a digenetic protozoan parasite causing leishmaniasis in humans . The different clinical forms of leishmaniasis are caused by more than twenty species of Leishmania that are transmitted by nearly thirty species of phlebotomine sand flies . Pentavalent antimonials ( such as Pentostam or Glucantime ) are the...
The degree of response to antimonial drugs varies widely between species and even among strains of the same species of the protozoan parasite Leishmania . However , the molecular mechanism ( s ) is unknown . In this study , we show that Leishmania aquaglyceroporin AQP1 drives this species-specific antimonial resistance...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Species-Specific Antimonial Sensitivity in Leishmania Is Driven by Post-Transcriptional Regulation of AQP1
Schmallenberg virus ( SBV ) is an emerging orthobunyavirus of ruminants associated with outbreaks of congenital malformations in aborted and stillborn animals . Since its discovery in November 2011 , SBV has spread very rapidly to many European countries . Here , we developed molecular and serological tools , and an ex...
Schmallenberg virus ( SBV ) was discovered in Germany ( near the town of Schmallenberg ) in November 2011 and since then has been found to be the cause of malformations and stillbirths in ruminants . SBV has spread very rapidly to many European countries including the Netherlands , Belgium , France and the United Kingd...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "rna", "viruses", "virology", "viral", "classification", "biology", "microbiology", "veterinary", "science" ]
2013
Schmallenberg Virus Pathogenesis, Tropism and Interaction with the Innate Immune System of the Host
In metazoans , the majority of mRNAs coding for secreted and membrane-bound proteins are translated on the surface of the endoplasmic reticulum ( ER ) . Although the targeting of these transcripts to the surface of the ER can be mediated by the translation of a signal sequence and their maintenance is mediated by inter...
Messenger RNAs ( mRNAs ) that encode secreted or membrane-bound proteins must be delivered to , and then maintained on , the surface of the endoplasmic reticulum ( ER ) . These mRNAs encode a short polypeptide that targets the mRNA/ribosome/nascent protein complexes to the ER surface during translation; however , recen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "rna", "cellular", "structures", "subcellular", "organelles", "rna", "transport", "cell", "biology", "nucleic", "acids", "protein", "translation", "gene", "expression", "membranes", "and", "sorting", "biology", "molecular", "cell", "biology", "molecular", "biology" ]
2012
p180 Promotes the Ribosome-Independent Localization of a Subset of mRNA to the Endoplasmic Reticulum
The contribution of basal and luminal cells to cancer progression and metastasis is poorly understood . We report generation of reporter systems driven by either keratin-14 ( K14 ) or keratin-8 ( K8 ) promoter that not only express a fluorescent protein but also an inducible suicide gene . Transgenic mice express the r...
Most , if not all , cancer-related deaths result from metastasis . The differentiation states of the cancer epithelial cells are thought to be a critical determinant of metastasis . Epithelial cancer cells with a basal cell type are more aggressive in forming metastasis than cancer cells with luminal cell type . Very l...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "breast", "tumors", "methods", "and", "resources", "toxins", "pathology", "and", "laboratory", "medicine", "respiratory", "infections", "cancers", "and", "neoplasms", "basic", "cancer", "research", "green", "fluorescent", "prot...
2018
Reporters to mark and eliminate basal or luminal epithelial cells in culture and in vivo
Eggs of the helminth Schistosoma mansoni accumulate in the colon following infection and generate Th2-biassed inflammatory granulomas which become down- modulated in size as the infection proceeds to chronicity . However , although CD4+CD25+FoxP3+regulatory T cells ( Tregs ) are known to suppress Th1-mediated colitis ,...
Schistosomiasis is an important parasitic helminth disease afflicting more than 200 million people worldwide . Infections are typically chronic and in the case of Schistosoma mansoni and S . japonicum the majority give rise to an intestinal form of disease caused by the deposition of parasite eggs in the colon and term...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "colon", "medicine", "infectious", "diseases", "schistosomiasis", "gastroenterology", "and", "hepatology", "neglected", "tropical", "diseases", "parasitic", "diseases" ]
2011
CD4+CD25+ Regulatory Cells Contribute to the Regulation of Colonic Th2 Granulomatous Pathology Caused by Schistosome Infection
Rift Valley fever virus is an arthropod-borne human and animal pathogen responsible for large outbreaks of acute and febrile illness throughout Africa and the Arabian Peninsula . Reverse genetics technology has been used to develop deletion mutants of the virus that lack the NSs and/or NSm virulence genes and have been...
Rift Valley fever virus is transmitted mainly by mosquitoes and causes disease in humans and animals throughout Africa and the Arabian Peninsula . The impact of disease is large in terms of human illness and mortality , and economic impact on the livestock industry . For these reasons , and because there is a risk of t...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "viral", "vaccines", "viral", "classification", "immunology", "microbiology", "vaccines", "rna", "viruses", "vaccination", "infectious", "diseases", "biology", "vectors", "and", "hosts", "mosquitoes", "clinical", "immunology", "immunity", "vector", "biology", ...
2012
Infection and Transmission of Rift Valley Fever Viruses Lacking the NSs and/or NSm Genes in Mosquitoes: Potential Role for NSm in Mosquito Infection
Understanding the pathogenesis of infection by neurotropic viruses represents a major challenge and may improve our knowledge of many human neurological diseases for which viruses are thought to play a role . Borna disease virus ( BDV ) represents an attractive model system to analyze the molecular mechanisms whereby a...
Neurotropic viruses have evolved diverse strategies to persist in their host , with variable consequences for brain function . The investigation of these mechanisms of persistence and associated disease represent a major issue in viral pathogenesis , as it may also improve our understanding of human neurological diseas...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/infectious", "diseases", "of", "the", "nervous", "system", "virology/persistence", "and", "latency", "virology/virulence", "factors", "and", "mechanisms", "infectious", "diseases/viral", "infections" ]
2009
Mutation of the Protein Kinase C Site in Borna Disease Virus Phosphoprotein Abrogates Viral Interference with Neuronal Signaling and Restores Normal Synaptic Activity
The generation and resolution of joint molecule recombination intermediates is required to ensure bipolar chromosome segregation during meiosis . During wild type meiosis in Caenorhabditis elegans , SPO-11-generated double stranded breaks are resolved to generate a single crossover per bivalent and the remaining recomb...
Meiotic recombination generates joint molecules that ensure chromosomes segregate correctly . Failure to generate or resolve joint molecules can have profound effects on fertility and on the viability of resulting progeny . The generation and resolution of joint molecules is carefully regulated . Generation of joint mo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2013
Joint Molecule Resolution Requires the Redundant Activities of MUS-81 and XPF-1 during Caenorhabditis elegans Meiosis
Sheep are a key source of meat , milk and fibre for the global livestock sector , and an important biomedical model . Global analysis of gene expression across multiple tissues has aided genome annotation and supported functional annotation of mammalian genes . We present a large-scale RNA-Seq dataset representing all ...
Sheep are ruminant mammals kept as livestock for the production of meat , milk and wool in agricultural industries across the globe . Genetic and genomic information can be used to improve production traits such as growth rate and health and fitness traits including disease resilience . The sheep genome is , however , ...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Methods" ]
[ "blood", "cells", "cdna", "libraries", "livestock", "medicine", "and", "health", "sciences", "reproductive", "system", "immune", "cells", "ruminants", "immunology", "vertebrates", "animals", "mammals", "forms", "of", "dna", "dna", "libraries", "dna", "mammalian", "g...
2017
A high resolution atlas of gene expression in the domestic sheep (Ovis aries)
MicroRNAs ( miRNA ) have emerged as key regulators of cell lineage differentiation and cancer . We used precursor miRNA profiling by a novel real-time QPCR method ( i ) to define progressive stages of endothelial cell transformation cumulating in Kaposi sarcoma ( KS ) and ( ii ) to identify specific miRNAs that serve a...
MicroRNAs are key regulators of cancer and development . We can use their pattern of expression to classify different cancers or in our case different stages in cancer development . We used a novel method to define progressive stages for Kaposi sarcoma ( KS ) , which is a cancer of the endothelial cells . We identified...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology/sarcomas", "oncology/skin", "cancers", "virology/viruses", "and", "cancer", "virology/effects", "of", "virus", "infection", "on", "host", "gene", "expression", "oncology/hematological", "malignancies" ]
2009
Pre-Micro RNA Signatures Delineate Stages of Endothelial Cell Transformation in Kaposi Sarcoma
How do bacteria regulate their cellular physiology in response to starvation ? Here , we present a detailed characterization of Escherichia coli growth and starvation over a time-course lasting two weeks . We have measured multiple cellular components , including RNA and proteins at deep genomic coverage , as well as l...
Bacteria frequently experience starvation conditions in their natural environments . Yet how they modify their physiology in response to these conditions remains poorly understood . Here , we performed a detailed , two-week starvation experiment in E . coli . We exhaustively monitored changes in cellular components , s...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Controlled Measurement and Comparative Analysis of Cellular Components in E. coli Reveals Broad Regulatory Changes in Response to Glucose Starvation
A new approach for the segregation of monaural sound mixtures is presented based on the principle of temporal coherence and using auditory cortical representations . Temporal coherence is the notion that perceived sources emit coherently modulated features that evoke highly-coincident neural response patterns . By clus...
Humans and many animals can effortlessly navigate complex sensory environments , segregating and attending to one desired target source while suppressing distracting and interfering others . In this paper , we present an algorithmic model that can accomplish this task with no prior information or training on complex si...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "auditory", "cortex", "machine", "learning", "algorithms", "neural", "networks", "engineering", "and", "technology", "noise", "control", "audio", "signal", "processing", "signal", "processing", "brain", "neuroscience", "hearing", "noise", "reduction", "artificial", "neu...
2014
Segregating Complex Sound Sources through Temporal Coherence
Visceral leishmaniasis ( VL ) is a major problem worldwide and causes significant morbidity and mortality . Existing drugs against VL have limitations , including their invasive means of administration long duration of treatment regimens . There are also concerns regarding increasing treatment relapses as well as the i...
Visceral Leishmaniasis remains a serious health problem in many developing countries with thousands of new cases recorded annually . Novel oral therapies are required as existing drugs are limited by their invasive means of administration long duration of treatment regimens . Moreover , with miltefosine as the sole ora...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "spleen", "immunology", "tropical", "diseases", "microbiology", "parasitic", "diseases", "protozoan", "life", "cycles", "parasitic", "protozoans", "developmental", "bio...
2019
Oral activity of the antimalarial endoperoxide 6-(1,2,6,7-tetraoxaspiro[7.11]nonadec-4-yl)hexan-1-ol (N-251) against Leishmania donovani complex
Rift Valley fever virus ( RVFV ) is a zoonotic arbovirus affecting livestock and people . This study was conducted in western Kenya where RVFV outbreaks have not previously been reported . The aims were to document the seroprevalence and risk factors for RVFV antibodies in a community-based sample from western Kenya an...
Rift Valley fever virus ( RVFV ) is a zoonotic virus affecting livestock and people . Periodic outbreaks in Kenya are associated with greater than average rainfall , although outbreaks have not previously been reported in western Kenya . The virus is spread between animals and to people by mosquitos . Contact with infe...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusion" ]
[ "livestock", "medicine", "and", "health", "sciences", "rift", "valley", "fever", "virus", "enzyme-linked", "immunoassays", "pathology", "and", "laboratory", "medicine", "ruminants", "pathogens", "geographical", "locations", "microbiology", "vertebrates", "animals", "mamma...
2017
The sero-epidemiology of Rift Valley fever in people in the Lake Victoria Basin of western Kenya
Podoconiosis , also known as mossy foot or endemic non-filarial elephantiasis , is a preventable form of lower-leg lymphoedema caused by prolonged ( typically barefoot ) exposure to soil derived from volcanic rocks . Acute adenolymphangitis ( also called ‘acute attack’ ) is a serious complication of podoconiosis result...
Podoconiosis is a foot condition , common in the highlands of Ethiopia , caused by exposure to volcanic soil . It can be prevented by wearing shoes and adhering to foot hygiene practices . Podoconiosis causes swelling of the lower legs and is a disabling and stigmatised condition . It is made worse by ‘acute attacks’ ,...
[ "Abstract", "Introduction", "Methods", "Discussion" ]
[ "children", "medicine", "and", "health", "sciences", "legs", "feet", "tropical", "diseases", "geographical", "locations", "social", "sciences", "parasitic", "diseases", "health", "care", "age", "groups", "neglected", "tropical", "diseases", "ethiopia", "body", "limbs"...
2019
The impact of acute adenolymphangitis in podoconiosis on caregivers: A case study in Wayu Tuka woreda, Oromia, Western Ethiopia. ‘If she was healthy, I would be free.’
Zika virus ( ZIKV ) , a member of the Flaviviridae family , is the most recent emerging arbovirus with pandemic potential . During infection , viruses trigger the host cell stress response , leading to changes in RNA translation and the assembly of large aggregates of stalled translation preinitiation complexes , terme...
Zika virus ( ZIKV ) is transmitted to humans primarily through mosquito bites , but there have also been cases of sexual , perinatal , and suspected blood transfusion transmission . It has been associated with fetal malformations and neurological disorders in adults . The rising concern about this pathogen led the Worl...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "phosphorylation", "infographics", "vero", "cells", "medicine", "and", "health", "sciences", "cellular", "stress", "responses", "pathology", "and", "laboratory", "medicine", "viral", "transmission", "and", "infection", "pathogens", "cell", "processes", "biological", "cu...
2017
Zika virus inhibits eIF2α-dependent stress granule assembly
Impaired nitric oxide ( NO˙ ) -cyclic guanosine 3' , 5'-monophosphate ( cGMP ) signaling has been observed in many cardiovascular disorders , including heart failure and pulmonary arterial hypertension . There are several enzymatic determinants of cGMP levels in this pathway , including soluble guanylyl cyclase ( sGC )...
Developing drugs for a well-defined biochemical or molecular pathway has conventionally been approached by optimizing the inhibition ( or activation ) of a single target by a single pharmacologic agent . On occasion , drug combinations have been used that generally target multiple pathways affecting a common phenotype ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "chemical", "compounds", "oxides", "pathology", "and", "laboratory", "medicine", "oxidative", "stress", "cardiovascular", "anatomy", "cgmp", "signaling", "neuroscience", "toxicology", "cell", "signaling", "toxicit...
2016
Systems Pharmacology and Rational Polypharmacy: Nitric Oxide−Cyclic GMP Signaling Pathway as an Illustrative Example and Derivation of the General Case
The accuracy of machine learning tasks critically depends on high quality ground truth data . Therefore , in many cases , producing good ground truth data typically involves trained professionals; however , this can be costly in time , effort , and money . Here we explore the use of crowdsourcing to generate a large nu...
Food security is a growing global concern . Farmers , plant breeders , and geneticists are hastening to address the challenges presented to agriculture by climate change , dwindling arable land , and population growth . Scientists in the field of plant phenomics are using satellite and drone images to understand how cr...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "machine", "learning", "algorithms", "engineering", "and", "technology", "applied", "mathematics", "signal", "processing", "crop", "genetics", "simulation", "and", "modeling", "algorithms", "research", "design", "plant", "science", "model", "organisms", "mathematics", "...
2018
Crowdsourcing image analysis for plant phenomics to generate ground truth data for machine learning
Heritability estimation provides important information about the relative contribution of genetic and environmental factors to phenotypic variation , and provides an upper bound for the utility of genetic risk prediction models . Recent technological and statistical advances have enabled the estimation of additive heri...
Heritability of a trait refers to the proportion of phenotypic variation that is due to genetic variation among individuals . It provides important information about the genetic basis of complex traits and indicates whether a phenotype is an appropriate target for more specific statistical and molecular genetic analyse...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome-wide", "association", "studies", "dermatology", "medicine", "and", "health", "sciences", "population", "genetics", "sociology", "social", "sciences", "neuroscience", "learning", "and", "memory", "skin", "neoplasms", "cognition", "genome", "analysis", "memory", "...
2017
Phenome-wide heritability analysis of the UK Biobank
Cockayne syndrome ( CS ) is a photosensitive , DNA repair disorder associated with progeria that is caused by a defect in the transcription-coupled repair subpathway of nucleotide excision repair ( NER ) . Here , complete inactivation of NER in Csbm/m/Xpa−/− mutants causes a phenotype that reliably mimics the human pro...
Normal metabolism routinely produces reactive oxygen species that damage DNA and other cellular components and is thought to contribute to the ageing process . Although DNA damage is typically kept in check by a variety of enzymes , several premature ageing disorders result from failure to remove damage from active gen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "geriatrics", "mammals", "diabetes", "and", "endocrinology", "computational", "biology", "mus", "(mouse)", "genetics", "and", "genomics" ]
2007
Impaired Genome Maintenance Suppresses the Growth Hormone–Insulin-Like Growth Factor 1 Axis in Mice with Cockayne Syndrome
Plants produce cytokinin ( CK ) hormones for controlling key developmental processes like source/sink distribution , cell division or programmed cell-death . Some plant pathogens have been shown to produce CKs but the function of this mimicry production by non-tumor inducing pathogens , has yet to be established . Here...
The role of plant-like hormonal compounds produced by fungal pathogens during infection has not been elucidated . Here we identified a conserved gene in most fungi , required for cytokinin production by the rice blast fungus and for its full virulence . Fungal-derived cytokinins are likely potent inhibitors of plant im...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "anatomy", "fungal", "genetics", "plant", "physiology", "hormones", "cereal", "crops", "fungi", "plant", "science", "rice", "model", "organisms", "plant", "hormones", "crops", "plant", "pathology", "plants", "research", "and", "analysis", "methods", "cytoki...
2016
Cytokinin Production by the Rice Blast Fungus Is a Pivotal Requirement for Full Virulence
Regulatory T ( Treg ) cells are known for their role in maintaining self-tolerance and balancing immune reactions in autoimmune diseases and chronic infections . However , regulatory mechanisms can also lead to prolonged survival of pathogens in chronic infections like leprosy and tuberculosis ( TB ) . Despite high hum...
Leprosy is a curable infectious disease caused by Mycobacterium leprae ( M . leprae ) that affects the skin and peripheral nerves . It is manifested in different forms ranging from self-healing , tuberculoid leprosy ( TT ) with low bacillary load and high cellular immunity against M . leprae , to lepromatous leprosy ( ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "medicine", "and", "health", "sciences", "biology", "and", "life", "sciences", "immunology", "microbiology" ]
2014
T-Cell Regulation in Lepromatous Leprosy
TNFα overexpression has been associated with several chronic inflammatory diseases , including psoriasis , lichen planus , rheumatoid arthritis , and inflammatory bowel disease . Paradoxically , numerous studies have reported new-onset psoriasis and lichen planus following TNFα antagonist therapy . Here , we show that ...
Psoriasis and lichen planus are chronic , debilitating skin diseases that affect millions of people worldwide . TNFα is a multifunctional cytokine that mediates acute and chronic inflammation . While TNFα antagonist therapy is used for autoimmune or chronic inflammatory diseases , such as inflammatory bowel disease ( I...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "dermatology", "inflammatory", "diseases", "innate", "immune", "system", "cellular", "stress", "responses", "medicine", "and", "health", "sciences", "immune", "cells", "cytokines", "cell", "processes", "immunology", "vertebrates", "animals", "epithelial", "cells", "oste...
2014
Tnfa Signaling Through Tnfr2 Protects Skin Against Oxidative Stress–Induced Inflammation
Rift Valley fever ( RVF ) , a mosquito-borne disease affecting ruminants and humans , is one of the most important viral zoonoses in Africa . The objective of the present study was to develop a geographic knowledge-based method to map the areas suitable for RVF amplification and RVF spread in four East African countrie...
Rift Valley fever ( RVF ) is a zoonotic disease affecting ruminants and humans . It occurs mostly in Africa , causing human deaths and important economic losses in the livestock sector . The RVF virus ( RVFV ) is transmitted from ruminant to ruminant by mosquitoes . Different climatic , environmental , and socio-econom...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion", "Conclusion" ]
[ "livestock", "medicine", "and", "health", "sciences", "rift", "valley", "fever", "virus", "pathology", "and", "laboratory", "medicine", "ruminants", "pathogens", "tropical", "diseases", "microbiology", "vertebrates", "geographical", "locations", "animals", "mammals", "v...
2016
Development and Assessment of a Geographic Knowledge-Based Model for Mapping Suitable Areas for Rift Valley Fever Transmission in Eastern Africa
F-box proteins share the F-box domain to connect substrates of E3 SCF ubiquitin RING ligases through the adaptor Skp1/A to Cul1/A scaffolds . F-box protein Fbx15 is part of the general stress response of the human pathogenic mold Aspergillus fumigatus . Oxidative stress induces a transient peak of fbx15 expression , re...
The opportunistic human fungal pathogen Aspergillus fumigatus is the most prevalent cause for severe fungal infections in immunocompromised hosts . A major virulence factor of A . fumigatus is its ability to rapidly adapt to host conditions during infection . The rapid response to environmental changes underlies a well...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "phosphorylation", "complement", "system", "aspergillus", "fumigatus", "medicine", "and", "health", "sciences", "immune", "physiology", "pathology", "and", "laboratory", "medicine", "aspergillus", "oxidative", "stress", "pathogens", "immunology", "microbiology", "aspergillu...
2016
SCF Ubiquitin Ligase F-box Protein Fbx15 Controls Nuclear Co-repressor Localization, Stress Response and Virulence of the Human Pathogen Aspergillus fumigatus
Covalent linkage to members of the small ubiquitin-like ( SUMO ) family of proteins is an important mechanism by which the functions of many cellular proteins are regulated . Sumoylation has roles in the control of protein stability , activity and localization , and is involved in the regulation of transcription , gene...
Proteins are subject to many types of modification that regulate their functions and which are applied after their initial synthesis in the cell . One such modification is known as sumoylation , the covalent linkage of a small ubiquitin-like protein to a wide variety of substrate proteins . Sumoylation is involved in t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Analysis of the SUMO2 Proteome during HSV-1 Infection
Among other effects , post-translational modifications ( PTMs ) have been shown to exert their function via the modulation of protein-protein interactions . For twelve different main PTM-types and associated subtypes and across 9 diverse species , we investigated whether particular PTM-types are associated with protein...
The function of proteins is frequently modulated by chemical modifications introduced after translation from RNA . These post-translational modifications ( PTMs ) have been shown to also influence the interaction between proteins carrying them . We tested whether specific PTM-types characterized by attaching different ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
The Roles of Post-translational Modifications in the Context of Protein Interaction Networks
Perception of microbe-associated molecular patterns ( MAMPs ) elicits transcriptional reprogramming in hosts and activates defense to pathogen attacks . The molecular mechanisms underlying plant pattern-triggered immunity remain elusive . A genetic screen identified Arabidopsis poly ( ADP-ribose ) glycohydrolase 1 ( at...
Fine-tuning of gene expression is a key feature of successful immune responses . However , the underlying mechanisms are not fully understood . Through a genetic screen in model plant Arabidopsis , we reveal that protein poly ( ADP-ribosyl ) ation ( PARylation ) post-translational modification plays a pivotal role in c...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry", "plant", "science", "genetics", "biology", "and", "life", "sciences", "immunology", "molecular", "biology" ]
2015
Protein Poly(ADP-ribosyl)ation Regulates Arabidopsis Immune Gene Expression and Defense Responses
The virulence of intracellular pathogens such as Leishmania major ( L . major ) relies largely on their ability to undergo cycles of replication within phagocytes , release , and uptake into new host cells . While all these steps are critical for successful establishment of infection , neither the cellular niche of eff...
Infection with Leishmania parasites can result in chronic disease of several months duration , often accompanied with disfiguring and disabling pathologies . Central to Leishmania virulence is the capability to survive and multiply within professional phagocytes . While it is assumed that the parasites at some point ha...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "blood", "cells", "fluorescence", "imaging", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "viral", "transmission", "and", "infection", "pathogens", "antigen-presenting", "cells", "immunology", "microbiology", ...
2018
CD11c-expressing Ly6C+CCR2+ monocytes constitute a reservoir for efficient Leishmania proliferation and cell-to-cell transmission
Snake bite is a neglected public health problem in communities in rural areas of several countries . Bothrops jararaca causes many snake bites in Brazil and previous studies have demonstrated that the pharmacological activities displayed by its venom undergo a significant ontogenetic shift . Similarly , the venom prote...
Bothrops jararaca is one of the most abundant venomous snake species in Brazil . It is primarily a nocturnal and generalist animal , however , it exhibits a notable ontogenetic shift in diet , feeding mainly on arthropods , lizards , and amphibians ( ectothermic prey ) through its juvenile phase and on small mammals ( ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biochemistry", "genetics", "biology", "proteomics", "genetics", "and", "genomics" ]
2012
A Transcriptomic View of the Proteome Variability of Newborn and Adult Bothrops jararaca Snake Venoms
Cyclic paroxysm and high fever are hallmarks of malaria and are associated with high levels of pyrogenic cytokines , including IL-1β . In this report , we describe a signature for the expression of inflammasome-related genes and caspase-1 activation in malaria . Indeed , when we infected mice , Plasmodium infection was...
Together Plasmodium falciparum and P . vivax infect approximately 250 million individuals , reaping life of near one million children every year . Extensive research on malaria pathogenesis has funneled into the consensus that the clinical manifestations are often a consequence of the systemic inflammation . Importantl...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunopathology", "inflammation", "immune", "cells", "cytokines", "monocytes", "immunity", "innate", "immunity", "immunology", "biology", "immune", "system" ]
2014
Malaria-Induced NLRP12/NLRP3-Dependent Caspase-1 Activation Mediates Inflammation and Hypersensitivity to Bacterial Superinfection
Enteropathogenic Escherichia coli ( EPEC ) disease depends on the transfer of effector proteins into epithelia lining the human small intestine . EPEC E2348/69 has at least 20 effector genes of which six are located with the effector-delivery system genes on the Locus of Enterocyte Effacement ( LEE ) Pathogenicity Isla...
Enteropathogenic Escherichia coli ( EPEC ) remain an important cause of infant diarrhoea and death in developing countries . Undoubtedly , a key pathogenic event relates to the bacteria's ability to inhibit the expression of anti-microbial and inflammation-inducing molecules regulated by the NF-κB transcription factor ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology", "microbiology", "host-pathogen", "interaction", "escherichia", "coli", "immunomodulation", "bacterial", "pathogens", "medical", "microbiology", "microbial", "pathogens", "biology", "pathogenesis", "molecular", "biology", "immune", "response", "signal", "transduc...
2011
The Enteropathogenic E. coli (EPEC) Tir Effector Inhibits NF-κB Activity by Targeting TNFα Receptor-Associated Factors
Even when antiretroviral therapy ( ART ) is started early after infection , HIV DNA might persist in the central nervous system ( CNS ) , possibly contributing to inflammation , brain damage and neurocognitive impairment . Paired blood and cerebrospinal fluid ( CSF ) were collected from 16 HIV-infected individuals on s...
Human Immunodeficiency virus ( HIV ) enters the central nervous system ( CNS ) early after infection and provides the basis for the development of neurocognitive impairment and potentially the establishment of latent reservoirs . Early initiation of antiretroviral therapy reduces HIV reservoir size in the periphery , b...
[ "Abstract", "Introduction", "Results", "Discussion", "Material", "and", "Methods" ]
[ "hiv", "infections", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "nervous", "system", "antiviral", "therapy", "pathogens", "immunology", "microbiology", "retroviruses", "viruses", "immunodeficiency", "viruses", ...
2017
Early Antiretroviral Therapy Is Associated with Lower HIV DNA Molecular Diversity and Lower Inflammation in Cerebrospinal Fluid but Does Not Prevent the Establishment of Compartmentalized HIV DNA Populations
Whole-exome or gene targeted resequencing in hundreds to thousands of individuals has shown that the majority of genetic variants are at low frequency in human populations . Rare variants are enriched for functional mutations and are expected to explain an important fraction of the genetic etiology of human disease , t...
Recent resequencing of the whole genome or the coding part of the genome ( the exome ) in thousands of individuals has described a large excess of low frequency variants in humans , probably arising as a consequence of recent rapid growth in human population sizes . Most rare variants are private to specific population...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Whole-Exome Sequencing Reveals a Rapid Change in the Frequency of Rare Functional Variants in a Founding Population of Humans
The influence of the mammalian retinal circadian clock on retinal physiology and function is widely recognized , yet the cellular elements and neural regulation of retinal circadian pacemaking remain unclear due to the challenge of long-term culture of adult mammalian retina and the lack of an ideal experimental measur...
The circadian clock in the mammalian retina regulates many retinal functions , and its output modulates the central circadian clock in the brain . Details about the cellular location and neural regulation of the mammalian retinal circadian clock remain unclear , however , largely due to the difficulty of maintaining lo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience" ]
2008
An Autonomous Circadian Clock in the Inner Mouse Retina Regulated by Dopamine and GABA
A major goal of contemporary studies of embryonic development is to understand large sets of regulatory changes that accompany the phenomenon of embryonic induction . The highly resolved sea urchin pregastrular endomesoderm–gene regulatory network ( EM-GRN ) provides a unique framework to study the global regulatory in...
In recent years , “gene regulatory networks” ( GRNs ) have provided integrated views of gene interactions that control biological processes . One of the earliest networks to be activated in the developing zygotes is the one controlling endomesoderm development . In the sea urchin , this network includes several subnetw...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "cell", "biology", "developmental", "biology" ]
2009
Gene Regulatory Network Interactions in Sea Urchin Endomesoderm Induction
A comparative analysis of cavities enclosed in a tertiary structure of proteins and interfaces formed by the interaction of two protein subunits in obligate and non-obligate categories ( represented by homodimeric molecules and heterocomplexes , respectively ) is presented . The total volume of cavities increases with ...
During protein folding a polypeptide chain takes up a three-dimensional structure that is characterized by close packing of atoms . For cellular processes proteins need to interact , and the binding is also characterized by packing of complementary surfaces . Two types of binding can be envisaged—obligate and non-oblig...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/macromolecular", "structure", "analysis" ]
2008
Cavities and Atomic Packing in Protein Structures and Interfaces
African trypanosomes constrain livestock and human health in Sub-Saharan Africa , and aggravate poverty and hunger of these otherwise largely livestock-keeping communities . To solve this , there is need to develop and use effective and cheap tsetse control methods . To this end , we aimed at determining the smallest p...
Poverty , hunger and human ill-health aggravated by trypanosomiasis in Sub-Saharan Africa can only be reduced by developing and using cheap and effective tsetse control methods . To further reduce the cost of tsetse control by restricting insecticides to the legs , belly and ears ( RAP ) we set out to determine the low...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biotechnology", "research", "design", "infectious", "diseases", "electrophoretic", "techniques", "veterinary", "science", "medicine", "and", "health", "sciences", "epidemiology", "mathematical", "and", "statistical", "techniques", "molecular", "biology", "techniques", "bio...
2014
Improvements on Restricted Insecticide Application Protocol for Control of Human and Animal African Trypanosomiasis in Eastern Uganda
Intellectual disability ( ID ) , one of the most common human developmental disorders , can be caused by genetic mutations in Cullin 4B ( Cul4B ) and cereblon ( CRBN ) . CRBN is a substrate receptor for the Cul4A/B-DDB1 ubiquitin ligase ( CRL4 ) and can target voltage- and calcium-activated BK channel for ER retention ...
Our study first demonstrates intellectual disability associated proteins , CRBN and BK channel pore-forming α subunit , are targeted by SCFFbxo7 for ubiquitination and proteolysis , which can be further regulated by their binding to DDB1 and the expression of CRL4 complex . We also first find DDB1 or CRBN deletion in m...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "calcium-activated", "potassium", "channels", "medicine", "and", "health", "sciences", "293t", "cells", "gene", "regulation", "enzymes", "biological", "cultures", "enzymology", "vertebrates", "electrophysiology", "mice", "neuroscience", "animals", "mammals", "learning", "...
2018
CRL4 antagonizes SCFFbxo7-mediated turnover of cereblon and BK channel to regulate learning and memory
A longstanding question in molecular biology is the extent to which the behavior of macromolecules observed in vitro accurately reflects their behavior in vivo . A number of sophisticated experimental techniques now allow the behavior of individual types of macromolecule to be studied directly in vivo; none , however ,...
The interior of a typical bacterial cell is a highly crowded place in which molecules must jostle and compete with each other in order to carry out their biological functions . The conditions under which such molecules are typically studied in vitro , however , are usually quite different: one or a few different types ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/molecular", "dynamics", "biophysics/macromolecular", "assemblies", "and", "machines", "biophysics/theory", "and", "simulation", "biophysics/protein", "folding" ]
2010
Diffusion, Crowding & Protein Stability in a Dynamic Molecular Model of the Bacterial Cytoplasm
The disc-large ( DLG ) –membrane-associated guanylate kinase ( MAGUK ) family of proteins forms a central signaling hub of the glutamate receptor complex . Among this family , some proteins regulate developmental maturation of glutamatergic synapses , a process vulnerable to aberrations , which may lead to neurodevelop...
During development , there are restricted time windows in which the brain’s cortex is reorganized; these changes depend on experience and serve to establish functionality . In immature brains , silent synapses—newly born synapses that are inactivated due to a lack of normal receptor-mediated signaling—are frequent . Re...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "nervous", "system", "brain", "social", "sciences", "electrophysiology", "neuroscience", "visual", "acuity", "vision", "neurotransmitters", "eyes", "animal", "cells", "visual", "cortex", "head", "glutamate", "bi...
2018
An opposing function of paralogs in balancing developmental synapse maturation
Drosophila provides an inexpensive and quantitative platform for measuring whole animal drug response . A complementary approach is virtual screening , where chemical libraries can be efficiently screened against protein target ( s ) . Here , we present a unique discovery platform integrating structure-based modeling w...
Effective and safe treatment of multigenic diseases often involves drugs that address multiple points along disease networks , i . e . , polypharmacology . Polypharmacology is increasingly appreciated as a potentially desirable property of kinase drugs . However , most known drugs that interact with multiple targets ha...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "chemical", "bonding", "enzymes", "enzymology", "animals", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "experimental", "organism", "systems", "gene", "types", "molecular", "biology", "techniques", "enzyme", "inhibitors", "droso...
2019
Integrated computational and Drosophila cancer model platform captures previously unappreciated chemicals perturbing a kinase network
The composition and structure of microbial communities associated with mosquitoes remain poorly understood despite their important role in host biology and potential to be harnessed as novel strategies for mosquito-borne disease control . We employed MiSeq sequencing of the 16S rRNA gene amplicons to characterize the b...
Knowledge of the microbial communities associated with disease vectors can be exploited for symbiotic control of vector-borne diseases . Here , we characterized and compared the bacterial communities of field-caught populations of Aedes mcintoshi and Aedes ochraceus , the primary vectors of Rift Valley fever ( RVF ) vi...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "rift", "valley", "fever", "virus", "microbiome", "pathology", "and", "laboratory", "medicine", "pathogens", "tropical", "diseases", "microbiology", "geographical", ...
2019
Host species and site of collection shape the microbiota of Rift Valley fever vectors in Kenya
South America has a complex demographic history shaped by multiple migration and admixture events in pre- and post-colonial times . Settled over 14 , 000 years ago by Native Americans , South America has experienced migrations of European and African individuals , similar to other regions in the Americas . However , th...
South America is home to over 400 million people who share a rich demographic history , including settlement by Native Americans , European colonization , and the African slave trade . We use genomic data to infer which populations from Europe and the Americas contributed to these admixture events . We provide evidence...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Genomic Insights into the Ancestry and Demographic History of South America
Type VI secretion ( T6S ) is a cell-to-cell injection system that can be used as a microbial weapon . T6S kills vulnerable cells , and is present in close to 25% of sequenced Gram-negative bacteria . To examine the ecological role of T6S among bacteria , we competed self-immune T6S+ cells and T6S-sensitive cells in sim...
Type VI secretion ( T6S ) is a cell-to-cell injection system that can be used as a microbial weapon . T6S kills vulnerable cells , and is present in a significant fraction of bacteria . Given the tactical advantage conferred by T6S , the system’s lack of universality suggests limits to its effectiveness relative to its...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Established Microbial Colonies Can Survive Type VI Secretion Assault
Publication of accurate and detailed descriptions of methods in research articles involving animals is essential for health scientists to accurately interpret published data , evaluate results and replicate findings . Inadequate reporting of key aspects of experimental design may reduce the impact of studies and could ...
There is a growing concern about animal use for scientific purposes . In order to maximize impact of results obtained and avoid unnecessary experiment repetition , certain strategies have been implemented such as publication of guidelines to encourage detailed reporting of animal studies . We contrasted information rep...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Quality of Reporting and Adherence to ARRIVE Guidelines in Animal Studies for Chagas Disease Preclinical Drug Research: A Systematic Review
β-Catenin , the core element of the Wnt/β-catenin pathway , is a multifunctional and evolutionarily conserved protein which performs essential roles in a variety of developmental and homeostatic processes . Despite its crucial roles , the mechanisms that control its context-specific functions in time and space remain l...
The Wnt signaling pathway is essential for proper intercellular communication in every developmental process since it controls basic cellular events as cell fate or proliferation . The key element of the Wnt signaling is β-catenin , which controls the transcription of multiple genes in the Wnt receiving cell . A main l...
[ "Abstract", "Introduction", "Results", "Discussion", "Conclusions", "Materials", "and", "methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "rna", "interference", "social", "sciences", "wnt", "signaling", "cascade", "neuroscience", "animals", "pigments", "signal", "inhibition", "materials", "science", "epigenetics", "transactivation", "eyes", "planaria...
2017
A C-terminally truncated form of β-catenin acts as a novel regulator of Wnt/β-catenin signaling in planarians
Toll-like receptor ( TLR ) -mediated signaling are critical for host defense against pathogen invasion . However , excessive responses would cause harmful damages to the host . Here we show that deficiency of the E3 ubiquitin ligase TRIM32 increases poly ( I:C ) - and LPS-induced transcription of downstream genes such ...
TLR3/4-mediated signaling needs to be effectively terminated to avoid excessive immune responses and harmful damages to the host . In this study , we provide genetic evidence to show that the E3 ubiquitin ligase TRIM32 negatively regulates TLR3/4-mediated innate immune and inflammatory responses . Trim32-/- mice are mo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "death", "medicine", "and", "health", "sciences", "autophagic", "cell", "death", "pathology", "and", "laboratory", "medicine", "molecular", "probe", "techniques", "pathogens", "immunology", "cell", "processes", "immunoblotting", "microbiology", "dna", "transcrip...
2017
TRIM32-TAX1BP1-dependent selective autophagic degradation of TRIF negatively regulates TLR3/4-mediated innate immune responses
Pseudomonas aeruginosa is a nearly ubiquitous human pathogen , and infections can be lethal to patients with impaired respiratory and immune systems . Prior studies have established that strong loss-of-function mutations in the egl-9 gene protect the nematode C . elegans from P . aeruginosa PAO1 fast killing . EGL-9 in...
Pseudomonas aeruginosa is a common bacterial pathogen that can infect a wide range of animals . In some conditions , P . aeruginosa produces cyanide , a toxin that limits cellular capacity to metabolize oxygen and produce energy . The nematode Caenorhabditis elegans is a powerful genetic model system for understanding ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/gene", "expression", "cell", "biology/cellular", "death", "and", "stress", "responses", "cell", "biology/developmental", "molecular", "mechanisms", "genetics", "and", "genomics/disease", "models", "infectious", "diseases/bacterial", "infections", ...
2010
C. elegans SWAN-1 Binds to EGL-9 and Regulates HIF-1-Mediated Resistance to the Bacterial Pathogen Pseudomonas aeruginosa PAO1
Several major human pathogens , including the filoviruses , paramyxoviruses , and rhabdoviruses , package their single-stranded RNA genomes within helical nucleocapsids , which bud through the plasma membrane of the infected cell to release enveloped virions . The virions are often heterogeneous in shape , which makes ...
Marburg virus and Ebola virus cause severe hemorrhagic fever in humans and non human primates . They are members of the family of Filoviridae , and part of the order Mononegavirales , which includes other important human pathogens: rabies virus , respiratory syncytial virus , and measles virus . All of these viruses co...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "viral", "transmission", "and", "infection", "macromolecular", "assemblies", "microbiology", "host-pathogen", "interaction", "viral", "structure", "viruslike", "particles", "protein", "structure", "membranes", "and", "sorting", "biology", "biophysics", "host", "cells", "c...
2011
Cryo-Electron Tomography of Marburg Virus Particles and Their Morphogenesis within Infected Cells
The radically distinct morphologies of arthropod and tetrapod legs argue that these appendages do not share a common evolutionary origin . Yet , despite dramatic differences in morphology , it has been known for some time that transcription factors encoded by the Distalless ( Dll ) /Dlx gene family play a critical role...
The development of vertebrate and invertebrate appendages differs in many respects . Yet , despite these differences , genes related to the Distalless ( Dll ) gene of Drosophila ( vertebrate Dlx genes ) are important for the development of appendages in multiple animal phyla . Such findings raise the question of whethe...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology/embryology", "developmental", "biology", "developmental", "biology/developmental", "evolution", "developmental", "biology/cell", "differentiation", "developmental", "biology/molecular", "development", "developmental", "biology/organogenesis" ]
2010
Non-Redundant Selector and Growth-Promoting Functions of Two Sister Genes, buttonhead and Sp1, in Drosophila Leg Development
Human alpha and beta defensins contribute substantially to innate immune defenses against microbial and viral infections . Certain nonhuman primates also produce theta-defensins—18 residue cyclic peptides that act as HIV-1 entry inhibitors . Multiple human theta-defensin genes exist , but they harbor a premature termin...
Defensins are a large family of small antimicrobial peptides that contribute to host defense against a broad spectrum of pathogens . In primates , defensins are divided into three subfamilies—alpha , beta , and theta—on the basis of their disulfide bonding pattern . Theta-defensins were the most recently identified def...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases", "immunology" ]
2009
Reawakening Retrocyclins: Ancestral Human Defensins Active Against HIV-1
Recombinant adenoviral ( rAd ) vectors elicit potent cellular and humoral immune responses and show promise as vaccines for HIV-1 , Ebola virus , tuberculosis , malaria , and other infections . These vectors are now widely used and have been generally well tolerated in vaccine and gene therapy clinical trials , with ma...
Recombinant adenovirus ( rAd ) vectors are remarkable for their ability to stimulate potent immune responses and to mediate highly efficient gene transfer . These vectors have been used extensively in human studies with generally acceptable tolerability . As with many bioactive compounds , adenoviruses can also cause p...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "immunology" ]
2007
Mechanism of Ad5 Vaccine Immunity and Toxicity: Fiber Shaft Targeting of Dendritic Cells
Understanding complexity in physical , biological , social and information systems is predicated on describing interactions amongst different components . Advances in genomics are facilitating the high-throughput identification of molecular interactions , and graphs are emerging as indispensable tools in explaining how...
The translation of genotype to phenotype is a tightly regulated process that is mediated by specific interactions between a variety of cellular components . Central to this is the transcription of genes , a process regulated by proteins that bind DNA , including the transcription factors ( TFs ) . Gene regulatory netwo...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "caenorhabditis", "gene", "regulation", "protein", "interaction", "networks", "brassica", "animals", "invertebrate", "genomics", "animal", "models", "fungi", "drosophila", "melanogaster", "model", "organisms", "caenorhabditis", "elegans", "scale-free", "net...
2018
Topological and statistical analyses of gene regulatory networks reveal unifying yet quantitatively different emergent properties
The Epstein-Barr virus ( EBV ) encoded oncoprotein Latent Membrane Protein 1 ( LMP1 ) signals through two C-terminal tail domains to drive cell growth , survival and transformation . The LMP1 membrane-proximal TES1/CTAR1 domain recruits TRAFs to activate MAP kinase , non-canonical and canonical NF-kB pathways , and is ...
The linear ubiquitin assembly complex ( LUBAC ) plays crucial roles in immune receptor-mediated NF-kB and MAP kinase pathway activation . Comparatively little is known about the extent to which microbial pathogens use LUBAC to activate downstream pathways . We demonstrate that TRAF1 enhances EBV oncoprotein LMP1 TES1/C...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation
The proper regulation of apoptosis requires precise spatial and temporal control of gene expression . While the transcriptional and translational activation of pro-apoptotic genes is known to be crucial to triggering apoptosis , how different mechanisms cooperate to drive apoptosis is largely unexplored . Here we repor...
Apoptosis , also referred to as programmed cell death , is a crucial cellular process that eliminates unwanted cells during animal development and tissue homeostasis . Abnormal regulation of apoptosis can cause developmental defects and a variety of other human disorders , including cancer , neurodegenerative diseases ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "model", "organisms", "genetics", "biology", "and", "life", "sciences", "research", "and", "analysis", "methods" ]
2014
The Translational Regulators GCN-1 and ABCF-3 Act Together to Promote Apoptosis in C. elegans
In Sub-Saharan Africa , infectious diarrhea is a major cause of morbidity and mortality . A case-control study was conducted to identify the etiology of diarrhea and to describe its main epidemiologic risk factors among hospitalized children under five years old in Bangui , Central African Republic . All consecutive ch...
Infectious diarrhea is a major cause of illness and death among children under five years from low-income country . In order to identify infectious agents associated with diarrhea , we conducted a case-control study in the Pediatric Complex of Bangui , the sole public pediatric hospital from Central African Republic ( ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2016
Etiology and Epidemiology of Diarrhea in Hospitalized Children from Low Income Country: A Matched Case-Control Study in Central African Republic
Recently we identified multiple suramin-sensitivity genes with a genome wide screen in Trypanosoma brucei that includes the invariant surface glycoprotein ISG75 , the adaptin-1 ( AP-1 ) complex and two deubiquitylating enzymes ( DUBs ) orthologous to ScUbp15/HsHAUSP1 and pVHL-interacting DUB1 ( type I ) , designated Tb...
The mechanisms by which pathogens interact with their environment are of major importance , both for fulfilling the basic needs of the parasite and understanding immune evasion . For African trypanosomes , the surface is dominated by the variant surface glycoprotein ( VSG ) , but recent data has demonstrated an importa...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Modulation of the Surface Proteome through Multiple Ubiquitylation Pathways in African Trypanosomes
Autophagy is a cellular process that is highly conserved among eukaryotes and permits the degradation of cellular material . Autophagy is involved in multiple survival-promoting processes . It not only facilitates the maintenance of cell homeostasis by degrading long-lived proteins and damaged organelles , but it also ...
Autophagy is a catabolic process involved in maintaining cellular homeostasis in eukaryotic cells , while coping with their changing environmental conditions . Mechanistically , it is also a process of considerable complexity involving multiple protein factors and implying numerous protein-protein and protein-membrane ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "parastic", "protozoans", "toxoplasma", "gondii", "protozoology", "biology", "microbiology" ]
2011
Autophagy Protein Atg3 is Essential for Maintaining Mitochondrial Integrity and for Normal Intracellular Development of Toxoplasma gondii Tachyzoites
The behavioral effects of psychomotor stimulants such as amphetamine ( AMPH ) arise from their ability to elicit increases in extracellular dopamine ( DA ) . These AMPH-induced increases are achieved by DA transporter ( DAT ) -mediated transmitter efflux . Recently , we have shown that AMPH self-administration is reduc...
Abuse of psychostimulants such as amphetamine remains a serious public health concern . Amphetamines mediate their behavioral effects by stimulating dopaminergic signaling throughout reward circuits of the brain . This property of amphetamine relies on its actions at the dopamine transporter ( DAT ) , a presynaptic pla...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neurological", "disorders", "physiology", "radiology", "and", "medical", "imaging", "neuroscience" ]
2007
Hypoinsulinemia Regulates Amphetamine-Induced Reverse Transport of Dopamine
Organisms respond to environmental changes by adapting the expression of key genes . However , such transcriptional reprogramming requires time and energy , and may also leave the organism ill-adapted when the original environment returns . Here , we study the dynamics of transcriptional reprogramming and fitness in th...
When microbes grow in a mixture of different nutrients , they repress the metabolism of nonpreferred nutrients such as complex carbohydrates until preferred nutrients , like glucose , are depleted . While this “catabolite repression” allows cells to use the most efficient nutrients first , it also comes at a cost becau...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "ecology", "microbial", "mutation", "model", "organisms", "microbial", "evolution", "gene", "expression", "genetics", "yeast", "and", "fungal", "models", "biology", "microbiology", "evolutionary", "biology", "microbial", "growth", "and", "development", "...
2014
Different Levels of Catabolite Repression Optimize Growth in Stable and Variable Environments
Optimal decision-making is based on integrating information from several dimensions of decisional space ( e . g . , reward expectation , cost estimation , effort exertion ) . Despite considerable empirical and theoretical efforts , the computational and neural bases of such multidimensional integration have remained la...
A major challenge for all organisms is selecting optimal behaviour to obtain resources while minimizing energetic and other expenses . Evolution provided mammals with exceptional decision-making capabilities to face this challenge . Even though neuroscientists have identified a heterogeneous and distributed set of brai...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "learning", "medicine", "and", "health", "sciences", "neurochemistry", "chemical", "compounds", "decision", "making", "brain", "social", "sciences", "neuroscience", "organic", "compounds", "learning", "and", "memory", "hormones", "simulation", "and", "modeling", "cognit...
2018
Dorsal anterior cingulate-brainstem ensemble as a reinforcement meta-learner
Influenza A virus usurps host signaling factors to regulate its replication . One example is mTOR , a cellular regulator of protein synthesis , growth and motility . While the role of mTORC1 in viral infection has been studied , the mechanisms that induce mTORC1 activation and the substrates regulated by mTORC1 during ...
Drug-resistant influenza viruses commonly arise due to frequent genetic changes and current antiviral drugs are not highly efficient . These underscore the need for new antiviral therapies effective against influenza viruses . Understanding how influenza virus uses cellular proteins for infection can potentially identi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "molecular", "probe", "techniques", "gene", "regulation", "pathogens", "microbiology", "signaling", "networks", "orthomyxoviruses", "viruses", "protein", "expression", ...
2017
Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication
Noise in the expression of a gene produces fluctuations in the concentration of the gene product . These fluctuations can interfere with optimal function or can be exploited to generate beneficial diversity between cells; gene expression noise is therefore expected to be subject to evolutionary pressure . Shifts betwee...
Noise in gene expression is important for phenotypic variation among genetically identical cells . The gene expression will be particularly sensitive to noise in transcription initiation . Transcription initiation from a given promoter involves multiple steps , each of which could be rate limiting . In this paper we di...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biochemistry/transcription", "and", "translation", "biophysics/transcription", "and", "translation", "biophysics/theory", "and", "simulation", "computational", "biology/transcriptional", "regulation" ]
2008
The Generation of Promoter-Mediated Transcriptional Noise in Bacteria
Bacterial whole genome sequencing offers the prospect of rapid and high precision investigation of infectious disease outbreaks . Close genetic relationships between microorganisms isolated from different infected cases suggest transmission is a strong possibility , whereas transmission between cases with genetically d...
Traditionally , outbreaks of infectious diseases are investigated by considering contact between cases and their exposure to possible sources of infection . This can be enhanced by using the genetic fingerprint of bacteria to rule out transmission between cases infected with unrelated strains . However , in some cases ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "bacterial", "diseases", "infectious", "diseases", "infectious", "disease", "control" ]
2013
Detection of Mixed Infection from Bacterial Whole Genome Sequence Data Allows Assessment of Its Role in Clostridium difficile Transmission
Honeybees , Apis mellifera , show age-related division of labor in which young adults perform maintenance ( “housekeeping” ) tasks inside the colony before switching to outside foraging at approximately 23 days old . Disease resistance is an important feature of honeybee biology , but little is known about the interact...
Honeybees have a highly developed form of social biology in which tasks are distributed among workers according to their age , with younger bees performing housekeeping tasks ( “house bees” ) before switching to foraging duties when they grow older . This division of labor is vital to colony function and survival . Pat...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "agroecology", "ecology", "immunology", "biology", "genomics", "zoology", "genetics", "and", "genomics", "agriculture" ]
2012
A Strong Immune Response in Young Adult Honeybees Masks Their Increased Susceptibility to Infection Compared to Older Bees
Mechanisms involved in severe P . vivax malaria remain unclear . Parasite polymorphisms , parasite load and host cytokine profile may influence the course of infection . In this study , we investigated the influence of circumsporozoite protein ( CSP ) polymorphisms on parasite load and cytokine profile in patients with...
Recent evidences have associated P . vivax infections with clinical complications , previously only attributed to P . falciparum malaria . The interaction between host and parasite may contribute to severity of the disease , however , the specific contribution of each factor remains unclear . Previous studies have show...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "innate", "immune", "system", "medicine", "and", "health", "sciences", "parasite", "groups", "immune", "physiology", "cytokines", "pathology", "and", "laboratory", "medicine", "parasite", "replication", "plasmodium", "immunology", "tropical", "diseases", "parasitic", "d...
2016
Polymorphisms in Plasmodium vivax Circumsporozoite Protein (CSP) Influence Parasite Burden and Cytokine Balance in a Pre-Amazon Endemic Area from Brazil
We seek to elucidate the role of macromolecular crowding in transcription and translation . It is well known that stochasticity in gene expression can lead to differential gene expression and heterogeneity in a cell population . Recent experimental observations by Tan et al . have improved our understanding of the func...
The cellular nucleus is packed with macromolecules such as DNAs and proteins , which leaves limited space for other molecules to move around . Recent experimental results by C . Tan et al . have shown that macromolecular crowding can regulate gene expression , resulting in a more homogenous cell population . We introdu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "engineering", "and", "technology", "gene", "regulation", "signal", "processing", "regulatory", "proteins", "dna-binding", "proteins", "noise", "reduction", "simulation", "and", "modeling", "systems", "science", "mathematics", "transcription", "factors", "epigenetics", "c...
2016
Macromolecular Crowding Regulates the Gene Expression Profile by Limiting Diffusion
In the last decades , several European countries where arboviral infections are not endemic have faced outbreaks of diseases such as chikungunya and dengue , initially introduced by infectious travellers from tropical endemic areas and then spread locally via mosquito bites . To keep in check the epidemiological risk ,...
Larvicides are a key tool to prevent the growth of mosquito populations and decrease both the risks of outbreaks of mosquito-borne diseases and the nuisance deriving from bites . In order to assist municipalities from temperate areas in Europe in effectively planning vector control programs , we modelled the effect of ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "larvicides", "invertebrates", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "togaviruses", "chikungunya", "infection", "cost-effectiveness", "analysis", "economic", "analysis", "pathogens", "tropical", "diseases", "microbiology", "g...
2017
Effectiveness and economic assessment of routine larviciding for prevention of chikungunya and dengue in temperate urban settings in Europe
Argasid ticks ( soft ticks ) are blood-feeding arthropods that can parasitize rodents , birds , humans , livestock and companion animals . Ticks of the Ornithodoros genus are known to be vectors of relapsing fever borreliosis in humans . In Algeria , little is known about relapsing fever borreliosis and other bacterial...
Argasid ticks ( soft ticks ) are obligate blood-feeding arthropods that may bite humans . To increase understanding of the bacteria that are associated with these arthropods , we collected soft ticks from different sites in Algeria and used molecular tools to detect and identify bacteria in these ticks . Molecular tool...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "taxonomy", "invertebrates", "borrelia", "infection", "medicine", "and", "health", "sciences", "ixodes", "pathology", "and", "laboratory", "medicine", "pathogens", "tropical", "diseases", "microbiology", "vertebrates", "animals", "mammals", "relapsing", "fever", "bacteria...
2017
Detection of relapsing fever Borrelia spp., Bartonella spp. and Anaplasmataceae bacteria in argasid ticks in Algeria
Understanding the heterogeneous nature of dengue transmission is important for prioritizing and guiding the implementation of prevention strategies . However , passive surveillance data in endemic countries are rarely adequately informative . We analyzed data from a cluster-sample , cross-sectional seroprevalence study...
Understanding the geographic distribution of dengue transmission intensity is of key importance for guiding dengue prevention strategies , including vaccination . We analyzed age-stratified data from a cross-sectional survey of 30 randomly selected urban sub-districts in Indonesia and estimated the force of infection (...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "urban", "geography", "medicine", "and", "health", "sciences", "enzyme-linked", "immunoassays", "immunology", "geographical", "locations", "spatial", "epidemiology", "social", "sciences", "indonesia", "pediatrics", "vaccines", "preventive", "medicine", "pediatric", "infecti...
2018
Geographic variation in dengue seroprevalence and force of infection in the urban paediatric population of Indonesia
Nontyphoidal Salmonellae commonly cause invasive disease in African children that is often fatal . The clinical diagnosis of these infections is hampered by the absence of a clear clinical syndrome . Drug resistance means that empirical antibiotic therapy is often ineffective and currently no vaccine is available . The...
Nontyphoidal Salmonellae are a major , yet often neglected , cause of fatal invasive disease among young African children , responsible for over 100 , 000 deaths a year . There is currently a lack of prospective studies to understand the clinical presentation and course of this disease . Diagnosis can be confusing , an...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion", "Conclusions" ]
[ "hiv", "infections", "medicine", "and", "health", "sciences", "body", "fluids", "pathology", "and", "laboratory", "medicine", "pathogens", "tropical", "diseases", "microbiology", "geographical", "locations", "parasitic", "diseases", "pulmonology", "retroviruses", "viruses...
2017
Presentation of life-threatening invasive nontyphoidal Salmonella disease in Malawian children: A prospective observational study