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Human co-infection with malaria and helmimths is ubiquitous throughout Africa . Nevertheless , its public health significance on malaria severity remains poorly understood . To contribute to a better understanding of epidemiology and control of this co-infection in Cameroon , a cross-sectional study was carried out to ...
Co-infection of malaria and intestinal helminths causes significant and additive problems against the host . In order to contribute to a better understanding of the epidemiology and control of concomitant intestinal geohelminthiasis and malaria infections in Cameroon , a cross-sectional study to assess its prevalence a...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biotechnology", "inflammatory", "diseases", "medicine", "and", "health", "sciences", "animal", "models", "of", "disease", "tropical", "diseases", "parasitic", "diseases", "parasitology", "gastroenterology", "and", "hepatology", "molecular", "biology", "techniques", "extr...
2014
Co-infections of Malaria and Geohelminthiasis in Two Rural Communities of Nkassomo and Vian in the Mfou Health District, Cameroon
Cryptococcus neoformans is one of the leading causes of invasive fungal infection in humans worldwide . C . neoformans uses macrophages as a proliferative niche to increase infective burden and avoid immune surveillance . However , the specific mechanisms by which C . neoformans manipulates host immunity to promote its...
Cryptococcus neoformans is an opportunistic fungal pathogen that is responsible for significant numbers of deaths in the immunocompromised population worldwide . Here we address whether eicosanoids produced by C . neoformans manipulate host innate immune cells during infection . Cryptococcus neoformans produces several...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "cryptococcus", "neoformans", "medicine", "and", "health", "sciences", "neurochemistry", "fish", "cryptococcus", "immune", "cells", "pathology", "and", "laboratory", "medicine", "enzymes", "pathogens", "eicosanoids", "immunology", "microbiology", "verteb...
2019
15-keto-prostaglandin E2 activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promote Cryptococcus neoformans growth during infection
Cholesteryl ester transfer protein ( CETP ) transports cholesteryl esters , triglycerides , and phospholipids between different lipoprotein fractions in blood plasma . The inhibition of CETP has been shown to be a sound strategy to prevent and treat the development of coronary heart disease . We employed molecular dyna...
Coronary heart disease is a major cause of death in the Western societies . One of the most promising interventions to prevent and slow down the progress of coronary heart disease is the elevation of high density lipoprotein ( HDL ) levels in circulation . Animal models together with early clinical studies have shown t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "computational", "biology", "biophysics" ]
2012
Lipid Exchange Mechanism of the Cholesteryl Ester Transfer Protein Clarified by Atomistic and Coarse-grained Simulations
Predicting organismal phenotypes from genotype data is important for plant and animal breeding , medicine , and evolutionary biology . Genomic-based phenotype prediction has been applied for single-nucleotide polymorphism ( SNP ) genotyping platforms , but not using complete genome sequences . Here , we report genomic ...
The ability to accurately predict values of complex phenotypes from genotype data will revolutionize plant and animal breeding , personalized medicine , and evolutionary biology . To date , genomic prediction has utilized high-density single-nucleotide polymorphism ( SNP ) genotyping arrays , but the availability of se...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biotechnology", "genome-wide", "association", "studies", "animal", "genetics", "statistics", "applied", "mathematics", "population", "genetics", "animal", "models", "genome", "sequencing", "algorithms", "drosophila", "melanogaster", "model", "organisms", "mathematics", "ef...
2012
Using Whole-Genome Sequence Data to Predict Quantitative Trait Phenotypes in Drosophila melanogaster
Kaposi’s sarcoma-associated herpesvirus ( KSHV ) is the infectious cause of the highly vascularized tumor Kaposi’s sarcoma ( KS ) , which is characterized by proliferating spindle cells of endothelial origin , extensive neo-angiogenesis and inflammatory infiltrates . The KSHV K15 protein contributes to the angiogenic a...
Both the latent and lytic replication phases of the KSHV life cycle are thought to contribute to its persistence and pathogenesis . The non-structural signaling membrane protein K15 is involved in the angiogenic and invasive properties of KSHV-infected endothelial cells . Here we show that the K15 protein is required f...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "transfection", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "biopsy", "gene", "regulation", "endothelial", "cells", "pathogens", "microbiology", "surgical", "and", "invasive", "medical", "procedures", "epitheli...
2017
The Kaposi's sarcoma-associated herpesvirus (KSHV) non-structural membrane protein K15 is required for viral lytic replication and may represent a therapeutic target
In a landmark paper , Nadeau and Taylor [18] formulated the random breakage model ( RBM ) of chromosome evolution that postulates that there are no rearrangement hotspots in the human genome . In the next two decades , numerous studies with progressively increasing levels of resolution made RBM the de facto theory of c...
Rearrangements are genomic “earthquakes” that change the chromosomal architectures . The fundamental question in molecular evolution is whether there exist “chromosomal faults” ( rearrangement hotspots ) where rearrangements are happening over and over again . The random breakage model ( RBM ) postulates that rearrange...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "evolutionary", "biology", "homo", "(human)", "mus", "(mouse)", "computational", "biology" ]
2007
Are There Rearrangement Hotspots in the Human Genome?
Frameshift and nonsense mutations are common in tumors with microsatellite instability , and mRNAs from these mutated genes have premature termination codons ( PTCs ) . Abnormal mRNAs containing PTCs are normally degraded by the nonsense-mediated mRNA decay ( NMD ) system . However , PTCs located within 50–55 nucleotid...
A class of mutations found in many cancers introduces aberrant termination signals during the synthesis of mRNA . In mammalian cells , abnormal mRNAs containing premature termination codons ( PTCs ) are normally degraded by a process called nonsense-mediated mRNA decay ( NMD ) , thus avoiding potentially deleterious ef...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "oncology", "cell", "biology", "in", "vitro", "molecular", "biology", "genetics", "and", "genomics" ]
2007
Selective Translational Repression of Truncated Proteins from Frameshift Mutation-Derived mRNAs in Tumors
The protease GP63 is an important virulence factor of Leishmania parasites . We previously showed that GP63 reaches the perinuclear area of host macrophages and that it directly modifies nuclear translocation of the transcription factors NF-κB and AP-1 . Here we describe for the first time , using molecular biology and...
Unicellular parasites of the genus Leishmania are the causative agent of leishmaniasis , a disease affecting 12 million people worldwide , mainly in tropical and subtropical regions of the developing world . They have evolved strategies to circumvent cellular defense mechanisms favouring their survival . This includes ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Impact of Leishmania Infection on Host Macrophage Nuclear Physiology and Nucleopore Complex Integrity
Survival at host temperature is a critical trait for pathogenic microbes of humans . Thermally dimorphic fungal pathogens , including Histoplasma capsulatum , are soil fungi that undergo dramatic changes in cell shape and virulence gene expression in response to host temperature . How these organisms link changes in te...
Microbial pathogens of humans display the ability to thrive at host temperature . So-called “thermally dimorphic” fungal pathogens , which include Histoplasma capsulatum , are a class of soil fungi that upon being inhaled into the human lung , undergo dramatic changes in cell shape and virulence gene expression in resp...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "signal", "transduction", "fungi", "mycology", "signaling", "in", "cellular", "processes", "molecular", "cell", "biology", "microbial", "physiology", "transcriptional", "signaling", "cellular", "stress", "responses", "medical", "microbiology", "gene", "expression", "micro...
2013
A Temperature-Responsive Network Links Cell Shape and Virulence Traits in a Primary Fungal Pathogen
Crimean-Congo hemorrhagic fever virus ( CCHFV ) is a tick-borne zoonotic agent that is maintained in nature in an enzootic vertebrate-tick-vertebrate cycle . Hyalomma genus ticks have been implicated as the main CCHFV vector and are key in maintaining silent endemic foci . However , what contributes to their central ro...
Crimean-Congo hemorrhagic fever virus ( CCHFV ) , a cause of severe hemorragic disease in humans , is maintained in nature in a tick-vertebrate-tick enzootic cycle characterized by silent persistence of endemic foci with intermittent epidemics . Most studies support ticks of the genus Hyalomma as the main CCHFV vectors...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "taxonomy", "invertebrates", "medicine", "and", "health", "sciences", "ixodes", "pathology", "and", "laboratory", "medicine", "vertebrates", "animals", "developmental", "biology", "phylogenetics", "data", "management", "nymphs", "network", "analysis", "ticks", "infectious...
2018
Host preferences support the prominent role of Hyalomma ticks in the ecology of Crimean-Congo hemorrhagic fever
Polymorphic inversions contribute to adaptation and phenotypic variation . However , large multi-centric association studies of inversions remain challenging . We present scoreInvHap , a method to genotype inversions from SNP data for genome-wide association studies ( GWASs ) , overcoming important limitations of curre...
Chromosomal inversions are structural variants consisting on an orientation change of a chromosome segment . Inversions have been linked to some phenotypic differences between individuals and to genetic divergence . However , their overall contribution to complex diseases is largely underdetermined as there are no high...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "pervasive", "developmental", "disorders", "variant", "genotypes", "geographical", "locations", "autism", "social", "sciences", "developmental", "psychology", "neuroscience", "genetic", "mappi...
2019
scoreInvHap: Inversion genotyping for genome-wide association studies
Cortical fast-spiking ( FS ) interneurons display highly variable electrophysiological properties . Their spike responses to step currents occur almost immediately following the step onset or after a substantial delay , during which subthreshold oscillations are frequently observed . Their firing patterns include high-...
About 25% of the neurons in the mammalian neocortex are inhibitory , namely reduce the activity of neurons they contact . These inhibitory neurons exhibit diversity of morphological , chemical , and biophysical properties , and their classification has recently been the focus of much debate . Even neurons belonging to ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience", "cortical", "fast-spiking", "neurons" ]
2007
Mechanisms of Firing Patterns in Fast-Spiking Cortical Interneurons
For many genes , proper gene expression requires coordinated and dynamic interactions between multiple regulatory elements , each of which can either promote or silence transcription . In Drosophila , the complexity of the regulatory landscape is further complicated by the tight physical pairing of homologous chromosom...
Gene regulation requires interactions between regions of DNA known as regulatory elements , which , in combination , determine where and when a gene will be active or silenced . Some genes use just a few regulatory elements , whereas others rely on highly complex interactions between many different elements that are po...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "gene", "regulation", "alleles", "animals", "invertebrate", "genomics", "dna", "transcription", "animal", "models", "drosophila", "melanogaster", "model", "organisms", "experimental", "organism", "systems", "eyes",...
2019
Two modes of transvection at the eyes absent gene of Drosophila demonstrate plasticity in transcriptional regulatory interactions in cis and in trans
A previous survey for clinical cases of Buruli ulcer ( BU ) in the Mapé Basin of Cameroon suggested that , compared to older age groups , very young children may be less exposed to Mycobacterium ulcerans . Here we determined serum IgG titres against the 18 kDa small heat shock protein ( shsp ) of M . ulcerans in 875 in...
Although M . ulcerans , the causative agent of Buruli ulcer ( BU ) , was identified in 1948 , its transmission pathways and environmental reservoirs remain poorly understood . The occurrence of M . ulcerans infections in endemic countries in West and Central Africa is highly focal and associated with stagnant and slow ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases", "medicine", "and", "health", "sciences", "emerging", "infectious", "diseases", "medical", "microbiology", "microbial", "pathogens", "biology", "and", "life", "sciences", "immunology", "microbiology", "bacterial", "pathogens", "immune", "response"...
2014
Late Onset of the Serological Response against the 18 kDa Small Heat Shock Protein of Mycobacterium ulcerans in Children
Neurocysticercosis ( NCC ) is a major cause of epilepsy in regions where pigs are free-ranging and hygiene is poor . Pork production is expected to increase in the next decade in sub-Saharan Africa , hence NCC will likely become more prevalent . In this study , people with epilepsy ( PWE , n = 212 ) were followed up 28...
Neurocysticercosis , a preventable and treatable disease , is one of the main causes of epilepsy in low income countries . In these countries , the diagnosis of epilepsy is often based on clinical presentation and interviews as neuroimaging is rarely available . It is crucial to distinguish people with epilepsy due to ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "public", "health", "and", "epidemiology", "neurocysticercosis", "infectious", "disease", "epidemiology", "test", "evaluation", "neglected", "tropical", "diseases", "population", "biology", "infectious", "diseases", "epilepsy", "epidemiology", "biology", "diagno...
2011
A Cross-Sectional Study of People with Epilepsy and Neurocysticercosis in Tanzania: Clinical Characteristics and Diagnostic Approaches
Percutaneous treatment ( PT ) emerged in the mid-1980s as an alternative to surgery for selected cases of abdominal cystic echinococcosis ( CE ) . Despite its efficacy and widespread use , the puncture of echinococcal cysts is still far from being universally accepted . One of the main reasons for this reluctance is th...
The risk of anaphylactic shock is the objection most often raised by opponents of percutaneous treatments for cystic echinococcosis , but there are no updated figures on the actual occurrence of anaphylaxis as a complication of this treatment . To assess the number of lethal and non-lethal anaphylactic reactions follow...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "echinococcosis", "infectious", "diseases", "neglected", "tropical", "diseases" ]
2011
Justified Concern or Exaggerated Fear: The Risk of Anaphylaxis in Percutaneous Treatment of Cystic Echinococcosis—A Systematic Literature Review
The systemic immune response of Drosophila is known to be induced both by septic injury and by oral infection with certain bacteria , and is characterized by the secretion of antimicrobial peptides ( AMPs ) into the haemolymph . To investigate other possible routes of bacterial infection , we deposited Erwinia carotovo...
Innate immunity is the first line of antimicrobial defence for vertebrates and the only immune response present in invertebrates such as the fruitfly Drosophila , which provides a powerful model system to study innate immunity . Interestingly , local infections of epithelia like the gut and , in our study , the genital...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/innate", "immunity", "genetics", "and", "genomics/genetics", "of", "the", "immune", "system" ]
2009
Long-Range Activation of Systemic Immunity through Peptidoglycan Diffusion in Drosophila
Mosquito sampling during Japanese encephalitis virus ( JEV ) -associated studies , particularly in India , has usually been conducted via aspirators or light traps to catch mosquitoes around cattle , which are dead-end hosts for JEV . High numbers of Culex tritaeniorhynchus , relative to other species , have often been...
The relative numbers of individuals of each mosquito species in an area are important to estimate when identifying species that contribute the most to vector-borne pathogen transmission . However , methods to sample mosquitoes and enumerate the number of individuals collected often vary in their catch efficacy between ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2016
Sampling Design Influences the Observed Dominance of Culex tritaeniorhynchus: Considerations for Future Studies of Japanese Encephalitis Virus Transmission
Members of the family Coronaviridae have the largest genomes of all RNA viruses , typically in the region of 30 kilobases . Several coronaviruses , such as Severe acute respiratory syndrome-related coronavirus ( SARS-CoV ) and Middle East respiratory syndrome-related coronavirus ( MERS-CoV ) , are of medical importance...
Ribosome profiling is emerging as a powerful technique to monitor translation in living cells at sub-codon resolution . It has particular applicability to virology , with the capacity to identify viral mRNAs that are being translated during infection and to provide new insights into virus gene expression , regulation a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "nucleic", "acid", "synthesis", "messenger", "rna", "negative-sense", "rna", "viruses", "viruses", "rna", "viruses", "genome", "analysis", "rna", "synthesis", "cellular", "structures", "and", "organelles", "chemical", "synthesis", "research", "and", "analysis", "metho...
2016
High-Resolution Analysis of Coronavirus Gene Expression by RNA Sequencing and Ribosome Profiling
Conservation outcomes are principally achieved through the protection of intact habitat or the restoration of degraded habitat . Restoration is generally considered a lower priority action than protection because protection is thought to provide superior outcomes , at lower costs , without the time delay required for r...
Most species go extinct because humans have cleared their habitat . Habitat loss can also cause people to lose some of the services provided by ecosystems , such as the removal of carbon dioxide from the atmosphere or the protection of coastal communities from storm damage . There are two broad strategies for stopping ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Dynamic", "Conservation", "Landscape", "Model" ]
[]
2015
Optimal Conservation Outcomes Require Both Restoration and Protection
Standard statistical approaches for prioritization of variants for functional testing in fine-mapping studies either use marginal association statistics or estimate posterior probabilities for variants to be causal under simplifying assumptions . Here , we present a probabilistic framework that integrates association s...
Genome-wide association studies ( GWAS ) have successfully identified numerous regions in the genome that harbor genetic variants that increase risk for various complex traits and diseases . However , it is generally the case that GWAS risk variants are not themselves causally affecting the trait , but rather , are cor...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetic", "polymorphism", "gene", "expression", "genetics", "gene", "regulation", "biology", "and", "life", "sciences", "population", "genetics", "locus", "control", "region", "evolutionary", "biology", "alleles", "genetic", "loci" ]
2014
Integrating Functional Data to Prioritize Causal Variants in Statistical Fine-Mapping Studies
Gliomas are a highly heterogeneous group of brain tumours that are refractory to treatment , highly invasive and pro-angiogenic . Glioblastoma patients have an average survival time of less than 15 months . Understanding the molecular basis of different grades of glioma , from well differentiated , low-grade tumours to...
Gliomas are aggressive brain tumours that are invasive , heterogeneous , refractory to treatment and show poor survival rates . Surgical resection and chemotherapy can increase patient survival but ultimately the disease is fatal . Multiple grades of glioma exist , with lower grades associated to better prognosis . Whi...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Inference of Low and High-Grade Glioma Gene Regulatory Networks Delineates the Role of Rnd3 in Establishing Multiple Hallmarks of Cancer
Coq6 is an enzyme involved in the biosynthesis of coenzyme Q , a polyisoprenylated benzoquinone lipid essential to the function of the mitochondrial respiratory chain . In the yeast Saccharomyces cerevisiae , this putative flavin-dependent monooxygenase is proposed to hydroxylate the benzene ring of coenzyme Q ( ubiqui...
Coenzyme Q is an essential redox active lipid present in most living organisms and in tissues of multicellular eukaryotes , which acts as a key component in the mitochondrial respiratory chain . It consists of an aromatic head and a long hydrophobic isoprenyl chain and is synthesized by several enzymes within an obliga...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[]
2016
Coenzyme Q Biosynthesis: Evidence for a Substrate Access Channel in the FAD-Dependent Monooxygenase Coq6
Previous theoretical studies of animal and human behavioral learning have focused on the dichotomy of the value-based strategy using action value functions to predict rewards and the model-based strategy using internal models to predict environmental states . However , animals and humans often take simple procedural be...
The neural mechanism of decision-making , a cognitive process to select one action among multiple possibilities , is a fundamental issue in neuroscience . Previous studies have revealed the roles of the cerebral cortex and the basal ganglia in decision-making , by assuming that subjects take a value-based reinforcement...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Parallel Representation of Value-Based and Finite State-Based Strategies in the Ventral and Dorsal Striatum
Arboviruses cycle through both vertebrates and invertebrates , which requires them to adapt to disparate hosts while maintaining genetic integrity during genome replication . To study the genetic mechanisms and determinants of these processes , we use chikungunya virus ( CHIKV ) , a re-emerging human pathogen transmitt...
Chikungunya ( CHIKV ) is a re-emerging mosquito-borne virus that constitutes a major and growing human health burden . Like all RNA viruses , during viral replication CHIKV copies its genome using a polymerase that makes an average of one mistake per replication cycle . Therefore , a single virus generates millions of ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "microbial", "mutation", "virology", "emerging", "viral", "diseases", "viral", "enzymes", "biology", "microbiology", "viral", "replication", "viral", "evolution" ]
2014
Alphavirus Mutator Variants Present Host-Specific Defects and Attenuation in Mammalian and Insect Models
The availability of a robust disease model is essential for the development of countermeasures for Middle East respiratory syndrome coronavirus ( MERS-CoV ) . While a rhesus macaque model of MERS-CoV has been established , the lack of uniform , severe disease in this model complicates the analysis of countermeasure stu...
The development of vaccines and treatment strategies is aided by robust animal disease models that accurately depict the illness that is observed in humans . Here we describe a new , improved model for MERS-CoV using the common marmoset , whereby the severe , and even lethal , illness that has been observed in many hum...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "primates", "animal", "models", "animal", "models", "of", "infection", "marmosets", "model", "organisms", "monkeys", "new", "world", "monkeys", "virology", "emerging", "viral", "diseases", "biology", "and", "life", "sciences", "microbiology", "vertebrates", "animals",...
2014
Infection with MERS-CoV Causes Lethal Pneumonia in the Common Marmoset
To navigate their surroundings , cells rely on sensory input that is corrupted by noise . In cells performing chemotaxis , such noise arises from the stochastic binding of signalling molecules at low chemoattractant concentrations . We reveal a fundamental relationship between the speed of chemotactic steering and the ...
Many cells can navigate upwards a concentration gradient of signalling molecules , a process called chemotaxis . Chemotaxis is used e . g . by sperm cells to find the egg . To measure and compare concentrations , cells count stochastic binding events of signalling molecules that diffuse to cellular receptors . Efficien...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "cell", "motility", "swimming", "medicine", "and", "health", "sciences", "steering", "decision", "making", "engineering", "and", "technology", "sperm", "chemotaxis", "signal", "processing", "social", "sciences", "neuroscience", "biological", "locomotion", "germ", "cells...
2018
Decision making improves sperm chemotaxis in the presence of noise
Phlebotomus duboscqi is the principle vector of Leishmania major , the causative agent of cutaneous leishmaniasis ( CL ) , in West Africa and is the suspected vector in Mali . Although found throughout the country the seasonality and infection prevalence of P . duboscqi has not been established in Mali . We conducted a...
Female sand flies transmit a parasite called Leishmania that causes a disease called cutaneous leishmaniasis ( CL ) . Several species of sand flies are found in West Africa , but only one species , Phlebotomus duboscqi , has been proven to transmit the parasite . Cutaneous Leishmaniasis has also been reported from Mali...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "ecology", "biology" ]
2011
Seasonality and Prevalence of Leishmania major Infection in Phlebotomus duboscqi Neveu-Lemaire from Two Neighboring Villages in Central Mali
Recent evidence suggests that the metabolism of some organisms , such as Escherichia coli , is remarkably efficient , producing close to the maximum amount of biomass per unit of nutrient consumed . This observation raises the question of what regulatory mechanisms enable such efficiency . Here , we propose that simple...
Bacteria live in remarkably diverse environments and constantly adapt to changing nutrient conditions . Recent evidence suggests that some bacteria , such as E . coli , are extraordinarily efficient in producing biomass under a variety of different nutrient conditions . This observation raises the question of what phys...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "computational", "biology/metabolic", "networks", "microbiology" ]
2010
Achieving Optimal Growth through Product Feedback Inhibition in Metabolism
The pathway for RNA interference is widespread in metazoans and participates in numerous cellular tasks , from gene silencing to chromatin remodeling and protection against retrotransposition . The unicellular eukaryote Trypanosoma cruzi is missing the canonical RNAi pathway and is unable to induce RNAi-related process...
Chagas' disease is a major health problem in Latin America and is caused by the protozoan parasite Trypanosoma cruzi . T . cruzi lacks the pathway for RNA interference , which is widespread among eukaryotes , and is therefore unable to induce RNAi-related processes . In many organisms , small RNAs play an important rol...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "and", "Discussion" ]
[ "genetics", "biology", "computational", "biology", "microbiology", "genetics", "and", "genomics" ]
2011
The Short Non-Coding Transcriptome of the Protozoan Parasite Trypanosoma cruzi
The BARD1 protein , which heterodimerizes with BRCA1 , is encoded by a known breast cancer susceptibility gene . While several BARD1 variants have been identified as pathogenic , many more missense variants exist that do not occur frequently enough to assign a clinical risk . In this paper , whole exome sequencing of o...
BARD1 is a breast cancer susceptibility gene encoding a protein that primarily interacts with BRCA1 in DNA repair . Although several BARD1 variants are known to be pathogenic , many more variants do not occur frequently enough to assign a clinical risk . In this paper , we identified 76 potentially cancer-associated BA...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "transfection", "medicine", "and", "health", "sciences", "3'", "utr", "gene", "regulation", "cancer", "risk", "factors", "messenger", "rna", "plasmid", "construction", "oncology", "dna", "damage", "ankyrin", "repeat", "domains", "untranslated", "regions", "dna", "co...
2019
Functional analysis of BARD1 missense variants in homology-directed repair and damage sensitivity
Schistosomiasis is a public health problem in Malawi but estimates of its prevalence vary widely . There is need for updated information on the extent of disease burden , communities at risk and factors associated with infection at the district and sub-district level to facilitate effective prioritization and monitorin...
Schistosoma hematobium infection is a parasitic infection endemic in Malawi . Schistosomiasis usually shows a focal distribution of infection and it is important to identify communities at high risk of infection and assess effectiveness of control programs . We conducted a survey in one district in Malawi to determine ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "public", "health", "and", "epidemiology/epidemiology", "public", "health", "and", "epidemiology/infectious", "diseases" ]
2009
Prevalence Distribution and Risk Factors for Schistosoma hematobium Infection among School Children in Blantyre, Malawi
Edwardsiella piscicida is a leading fish pathogen that causes significant economic loses in the aquaculture industry . The pathogen depends on type III and type VI secretion systems ( T3/T6SS ) for growth and virulence in fish and the expression of both systems is controlled by the EsrB transcription activator . Here ,...
Edwardsiella piscicida , a major fish pathogen , relies on T3/T6SSs for virulence and the EsrB transcription activator promotes the expression of these secretion systems and many other genes that enable growth in fish . Here , we found that the alternative sigma factor RpoS inhibits expression of esrB thereby diminishi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "microbiology", "dna", "transcription", "bacterial", "diseases", "regulator", "genes", "enterobacteriaceae", "genetic", "elements", "gene", "types", ...
2018
Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida
Lowe Syndrome is a developmental disorder characterized by eye , kidney , and neurological pathologies , and is caused by mutations in the phosphatidylinositol-5-phosphatase OCRL . OCRL plays diverse roles in endocytic and endolysosomal trafficking , cytokinesis , and ciliogenesis , but it is unclear which of these cel...
Lowe syndrome is a developmental disorder characterized by severe kidney , eye , and neurological symptoms , and is caused by mutations in the gene OCRL . OCRL has been shown to control many steps of packaging and transport of materials within cells , though it remains unclear which of these disrupted transport steps c...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "cell", "physiology", "medicine", "and", "health", "sciences", "immune", "cells", "vesicles", "immunology", "developmental", "biology", "cellular", "structures", "and", "organelles", "endosomes", "white", "blood", "cells", "animal", "cells", "life", ...
2017
dOCRL maintains immune cell quiescence by regulating endosomal traffic
Plasmodium relapses are attributed to the activation of dormant liver-stage parasites and are responsible for a significant number of recurring malaria blood-stage infections . While characteristic of human infections caused by P . vivax and P . ovale , their relative contribution to malaria disease burden and transmis...
Plasmodium vivax contributes significantly to global malaria morbidity and remains a major obstacle for malaria elimination due to its ability to form dormant stages in the liver . These forms can become activated to cause relapsing blood-stage infections . Relapses remain poorly understood because it is difficult to v...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "parasite", "groups", "immune", "cells", "plasmodium", "gametocytes", "immunology", "tropical", "diseases", "vertebrates", "parasitic", "diseases", "parasitic", "protozoans", "animals", "parasitology", "mammals", ...
2019
Humoral immunity prevents clinical malaria during Plasmodium relapses without eliminating gametocytes
Cells and tissues are exposed to stress from numerous sources . Senescence is a protective mechanism that prevents malignant tissue changes and constitutes a fundamental mechanism of aging . It can be accompanied by a senescence associated secretory phenotype ( SASP ) that causes chronic inflammation . We present a Boo...
The senescence associated secretory phenotype is developed by cells undergoing permanent cell cycle arrest . This phenotype is characterized by the secretion of a variety of factors that facilitate tissue breakdown and inflammation and is therefore theorized to , in part , be causal for aging and age-related diseases ....
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "senescence", "gene", "regulation", "cell", "cycle", "and", "cell", "division", "cell", "processes", "fibroblasts", "dna", "damage", "physiological", "processes", "developmental", "biology", "organism", "development", "network", ...
2017
A model of the onset of the senescence associated secretory phenotype after DNA damage induced senescence
Leprosy is endemic in large part of Brazil with 28 , 761 new patients in 2015 , the second largest number worldwide and reaches 9/10 . 000 in highly endemic regions and 2 . 7/10 . 000 in the city of Fortaleza , Ceará , Northeast Brazil . For better understanding of risk factors for leprosy transmission , we conducted a...
Leprosy is a transmissible disease that is still endemic in several countries including in Brazil , a country with highly variable region associated incidence of disease . Fortaleza is a city in Northeast Brazil with high incidence and conventional epidemiology studies are suggestive for high levels of recent transmiss...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "biogeography", "mycobacterium", "leprae", "medicine", "and", "health", "sciences", "ecology", "and", "environmental", "sciences", "biopsy", "population", "genetics", "tropical", "diseases", "geographical", "locations", "surgical", "and", "invasive", "medical", "procedure...
2017
Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
Iron is essential for life . Accessing iron from the environment can be a limiting factor that determines success in a given environmental niche . For bacteria , access of chelated iron from the environment is often mediated by TonB-dependent transporters ( TBDTs ) , which are β-barrel proteins that form sophisticated ...
Earth’s carbon cycle depends on saprophytic microbes to rot old or diseased plant matter and recycle carbon from that biomass . Some bacteria ( phytopathogens ) have evolved to cause disease and rot in even healthy plants and may have utility as biological control agents against noxious weeds . To understand the mechan...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "crystal", "structure", "markov", "models", "enzymes", "condensed", "matter", "physics", "enzymology", "membrane", "proteins", "crystals", "plant", "science", "mathematics", "outer", "membrane", "proteins", "materials", "science", "plant", "pathology", "crystallography", ...
2018
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants
The vertebrate cranium is a prime example of the high evolvability of complex traits . While evidence of genes and developmental pathways underlying craniofacial shape determination is accumulating , we are still far from understanding how such variation at the genetic level is translated into craniofacial shape variat...
Formation of the face , mandible , and skull is determined in part by genetic factors , but the relationship between genetic variation and craniofacial development is not well understood . We demonstrate how recent advances in mouse genomics and statistical methods can be used to identify genes involved in craniofacial...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Mapping of Craniofacial Traits in Outbred Mice Identifies Major Developmental Genes Involved in Shape Determination
Accurate quantitative assessment of infection with soil transmitted helminths and protozoa is key to the interpretation of epidemiologic studies of these parasites , as well as for monitoring large scale treatment efficacy and effectiveness studies . As morbidity and transmission of helminth infections are directly rel...
Gastrointestinal parasites including soil-transmitted helminths cause considerable morbidity worldwide , especially in resource-poor communities . Large-scale epidemiologic and treatment efficacy studies are regularly undertaken to determine the optimum ways to reduce or eliminate parasites from endemic communities , t...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "giardia", "parasitic", "diseases", "parasitic", "protozoans", "animals", "necator", "americanus", "health", "care", "ascaris", "protozoans", "molecular", "biology", "techniques", "morbidity", "research", "and", ...
2016
Application of a Multiplex Quantitative PCR to Assess Prevalence and Intensity Of Intestinal Parasite Infections in a Controlled Clinical Trial
In Latin America , there are 13 geographically isolated endemic foci distributed among Mexico , Guatemala , Colombia , Venezuela , Brazil and Ecuador . The communities of the three endemic foci found within Mexico have been receiving ivermectin treatment since 1989 . In this study , we predicted the trend of occurrence...
Mexico is one of the countries where human onchocerciasis ( river blindness ) can be found in Latin America . In 1989 , the onchocerciasis program in Mexico started the treatment with ivermectin only for symptomatic individuals and then mass distribution of ivermectin was initiated for all eligible residents from 1994 ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "public", "health", "and", "epidemiology", "epidemiology", "infectious", "disease", "epidemiology", "epidemiological", "methods", "public", "health" ]
2013
Time Series Analysis of Onchocerciasis Data from Mexico: A Trend towards Elimination
Toll-like receptors ( TLR ) and cytokines play a central role in the pathogen clearance as well as in pathological processes . Recently , we reported that TLR2 , TLR4 and TLR9 are differentially modulated in injured livers from BALB/c and C57BL/6 ( B6 ) mice during Trypanosoma cruzi infection . However , the molecular ...
Trypanosoma cruzi , an obligate intracellular protozoan , is the etiological agent of Chagas Disease that represents an important public health burden in Latin America . The infection with this parasite can lead to severe complications in cardiac , liver and gastrointestinal tissue depending on the strain of parasite a...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/protozoal", "infections", "infectious", "diseases/neglected", "tropical", "diseases", "immunology/immunity", "to", "infections", "immunology/innate", "immunity" ]
2010
Importance of TLR2 on Hepatic Immune and Non-Immune Cells to Attenuate the Strong Inflammatory Liver Response During Trypanosoma cruzi Acute Infection
The interplay of microbiota and the human host is physiologically crucial in health and diseases . The beneficial effects of lactic acid bacteria ( LAB ) , permanently colonizing the human intestine or transiently obtained from food , have been extensively reported . However , the molecular understanding of how LAB mod...
Although it has been known for 15 years that HCA3 is present in humans and other hominids but absent in all other mammals , no study so far aimed to understand why HCA3 was functionally preserved during evolution . Here , we take advantage of evolutionary analyses which we combine with functional assays of hominid HCA3...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "body", "fluids", "immunology", "vertebrates", "animals", "mammals", "urine", "primates", "metabolites", "gibbons", "digestive", "system", "white", "blood", "cells", "animal", "cells", "ev...
2019
Metabolites of lactic acid bacteria present in fermented foods are highly potent agonists of human hydroxycarboxylic acid receptor 3
Efficient HTLV-1 viral transmission occurs through cell-to-cell contacts . The Tax viral transcriptional activator protein facilitates this process . Using a comparative transcriptomic analysis , we recently identified a series of genes up-regulated in HTLV-1 Tax expressing T-lymphocytes . We focused our attention towa...
HTLV-1 was the first human oncoretrovirus to be discovered . Five to ten million people are infected , and 1–6% will develop either Adult T-cell Leukemia , or Tropical Spastic Paraparesis/HTLV-1 Associated Myelopathy ( TSP/HAM ) . HTLV-1 infects primarily T-cells , but dendritic cells were also found to carry proviruse...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "viral", "transmission", "and", "infection", "viruses", "and", "cancer", "virology", "gene", "expression", "biology", "microbiology", "molecular", "cell", "biology" ]
2014
Gem-Induced Cytoskeleton Remodeling Increases Cellular Migration of HTLV-1-Infected Cells, Formation of Infected-to-Target T-Cell Conjugates and Viral Transmission
Replicating recombinant vector vaccines consist of a fully competent viral vector backbone engineered to express an antigen from a foreign transgene . From the perspective of viral replication , the transgene is not only dispensable but may even be detrimental . Thus vaccine revertants that delete or inactivate the tra...
Recombinant vector vaccines are live replicating viruses that are engineered to carry extra genes derived from a pathogen—and these extra genes produce proteins against which we want to generate immunity . These vaccine genomes may evolve to lose the extra genes during the process of manufacture of the vaccine or durin...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "organismal", "evolution", "viral", "vaccines", "medicine", "and", "health", "sciences", "immunology", "microbiology", "vaccines", "microbial", "evolution", "infectious", "disease", "control", "infectious", "diseases", "evolutionary", "immunology", "viral", "evolution", "...
2019
Recombinant vector vaccine evolution
Plant protein kinases form redundant signaling pathways to perceive microbial pathogens and activate immunity . Bacterial pathogens repress cellular immune responses by secreting effectors , some of which bind and inhibit multiple host kinases . To understand how broadly bacterial effectors may bind protein kinases and...
Some bacterial pathogens secrete virulence factors called effectors , which influence host tissues during infection . The impact of such bacterial effectors on the transmission of immune signals in plants remains poorly understood . In this study , we developed an integrative network approach to discover interactions b...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "death", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "cell", "processes", "immunology", "signaling", "networks", "microbiology", "cell-mediated", "immunity", "network", "analysis", "plants", "flowering", "p...
2018
Integrative network-centric approach reveals signaling pathways associated with plant resistance and susceptibility to Pseudomonas syringae
Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development . This study aimed to investigate the relationship between total IgG and the IgG subclasses and the monocyte IgG receptor , known as FcγRIIIa or CD16 , in schis...
Schistosomiasis is a parasitic disease caused by the parasite Schistosoma spp . Over 240 million people are infected worldwide , mainly in Sub-Saharan Africa , but an efficacious , protective vaccine has yet to be found . Protection against schistosome infection in individuals living in endemic areas is mediated by ant...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "humoral", "immunity", "innate", "immune", "system", "immune", "cells", "antigen-presenting", "cells", "immunology", "microbiology", "parasitology", "antibodies", "immune", "system", "proteins", "white", "blood", "cells", "animal", "cells", "proteins",...
2014
CD16 Expression on Monocytes in Healthy Individuals but Not Schistosome-Infected Patients Is Positively Associated with Levels of Parasite-Specific IgG and IgG1
Kaposi sarcoma-associated herpesvirus ( KSHV ) causes several tumors and hyperproliferative disorders . Hypoxia and hypoxia-inducible factors ( HIFs ) activate latent and lytic KSHV genes , and several KSHV proteins increase the cellular levels of HIF . Here , we used RNA sequencing , qRT-PCR , Taqman assays , and path...
Kaposi sarcoma-associated herpesvirus ( KSHV ) is an oncogenic herpesvirus known to cause several tumors and hyperproliferative disorders . While there has been reports of KSHV activating and increasing hypoxia-inducible factors ( HIFs ) , this is the first report investigating and establishing the extent to which KSHV...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "sequencing", "techniques", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "gene", "regulation", "pathogens", "rna", "extraction", "microbiology", "pulmonology", "viruses", "micrornas", "dna", "viruses", "hypoxia", "molecular", "b...
2017
RNA Sequencing Reveals that Kaposi Sarcoma-Associated Herpesvirus Infection Mimics Hypoxia Gene Expression Signature
Coincidence detector neurons transmit timing information by responding preferentially to concurrent synaptic inputs . Principal cells of the medial superior olive ( MSO ) in the mammalian auditory brainstem are superb coincidence detectors . They encode sound source location with high temporal precision , distinguishin...
Brain cells ( neurons ) are spatially extended structures . The locations at which neurons receive inputs and generate outputs are often distinct . We formulate and study a minimal mathematical model that describes the dynamical coupling between the input and output regions of a neuron . We construct our model to refle...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "pharmacologic", "analysis", "action", "potentials", "medicine", "and", "health", "sciences", "dendritic", "structure", "auditory", "nerves", "nervous", "system", "membrane", "potential", "electrophysiology", "neuroscience", "simulation", "and", "modeling", "compartment", ...
2019
Soma-axon coupling configurations that enhance neuronal coincidence detection
Bacterial growth depends crucially on metabolic fluxes , which are limited by the cell’s capacity to maintain metabolic enzymes . The necessary enzyme amount per unit flux is a major determinant of metabolic strategies both in evolution and bioengineering . It depends on enzyme parameters ( such as kcat and KM constant...
“Enzyme cost” , the amount of protein needed for a given metabolic flux , is crucial for the metabolic choices cells have to make . However , due to the technical limitations of linear optimization methods , this cost has traditionally been ignored by constraint-based metabolic models such as Flux Balance Analysis . On...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "protein", "metabolism", "enzymes", "enzymology", "vertebrates", "animals", "mammals", "metabolites", "enzyme", "metabolism", "enzyme", "kinetics", "thermodynamics", "enzyme", "chemistry", "proteins", "metabolic", "pathways", "metabolism", "physics", "biochemistry", "cats",...
2016
The Protein Cost of Metabolic Fluxes: Prediction from Enzymatic Rate Laws and Cost Minimization
The skin secretion of many amphibians contains an arsenal of bioactive molecules , including hormone-like peptides ( HLPs ) acting as defense toxins against predators , and antimicrobial peptides ( AMPs ) providing protection against infectious microorganisms . Several amphibian taxa seem to have independently acquired...
Many amphibians defend themselves against predation and infections by secreting a mixture of gene-encoded toxins and antimicrobials . How does such an integrated defense weapon arise and how does it diversify to gain distinct antipredatory and antimicrobial functions ? We took advantage of the availability of a sequenc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "biology", "zoology", "biology", "genomics" ]
2013
Origin and Functional Diversification of an Amphibian Defense Peptide Arsenal
Myelin is required for proper nervous system function . Schwann cells in developing nerves depend on extrinsic signals from the axon and from the extracellular matrix to first sort and ensheathe a single axon and then myelinate it . Neuregulin 1 type III ( Nrg1III ) and laminin α2β1γ1 ( Lm211 ) are the key axonal and m...
Myelin is formed by the wrapping of glial cell membranes around axons and is required for the fast conduction of nerve impulses and to support axons . In the peripheral nervous system , myelin is produced by Schwann cells . Peripheral myelin defects cause debilitating diseases , whose molecular pathogeneses are only pa...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "phosphorylation", "medicine", "and", "health", "sciences", "nervous", "system", "neuroscience", "signal", "inhibition", "nerve", "fibers", "protein", "kinase", "signaling", "cascade", "animal", "cells", "axons", "proteins", "biochemistry", "signal", "transduction", "ce...
2017
Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination
Methodology to estimate malaria incidence rates from a commonly occurring form of interval-censored longitudinal parasitological data—specifically , 2-wave panel data—was first proposed 40 years ago based on the theory of continuous-time homogeneous Markov Chains . Assumptions of the methodology were suitable for setti...
Incidence rates measure the transitions between the states of noninfected to infected per unit of time and per person at risk . Usually calculated from longitudinal observations , they provide an indication of how rapidly a disease develops in a population over time . In the context of malaria , longitudinal data on in...
[ "Abstract", "Introduction", "Methods", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "markov", "models", "atmospheric", "science", "drugs", "tropical", "diseases", "social", "sciences", "parasitic", "diseases", "parasitic", "protozoans", "animals", "antimalarials", "mathematics", "protozoans", "pha...
2016
Malaria Incidence Rates from Time Series of 2-Wave Panel Surveys
Translesion synthesis ( TLS ) enables DNA replication through damaged bases , increases cellular DNA damage tolerance , and maintains genomic stability . The sliding clamp PCNA and the adaptor polymerase Rev1 coordinate polymerase switching during TLS . The polymerases Pol η , ι , and κ insert nucleotides opposite dama...
DNA translesion synthesis ( TLS ) allows the DNA replication machinery to replicate past damaged bases , thus increasing cellular tolerance for DNA damage and maintaining genomic stability . Suppression of TLS is expected to enhance the efficacy of the anti-cancer drug , cisplatin . TLS employs a special set of DNA pol...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "biochemistry", "biology", "and", "life", "sciences" ]
2014
The Transcription Factor TFII-I Promotes DNA Translesion Synthesis and Genomic Stability
Enterohemorrhagic Escherichia coli ( EHEC ) is one major type of contagious and foodborne pathogens . The type VI secretion system ( T6SS ) has been shown to be involved in the bacterial pathogenicity and bacteria-bacteria competition . Here , we show that EHEC could secrete a novel effector KatN , a Mn-containing cata...
The type VI secretion system ( T6SS ) is a specific macromolecular protein export apparatus , and widely distributed in Gram-negative bacteria . Generally , T6SS has been shown to play an important role in anti-bacterial competition and virulence to eukaryotic hosts . Enterohemorrhagic Escherichia coli ( EHEC ) can cau...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "bacteriology", "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "pathology", "and", "laboratory", "medicine", "intracellular", "pathogens", "enzymes", "pathogens", "immunology", "enzymology", "microbiology", "physiological", "processes", "bac...
2017
Type VI secretion system contributes to Enterohemorrhagic Escherichia coli virulence by secreting catalase against host reactive oxygen species (ROS)
HIV-1 is present in anatomical compartments and bodily fluids . Most transmissions occur through sexual acts , making virus in semen the proximal source in male donors . We find three distinct relationships in comparing viral RNA populations between blood and semen in men with chronic HIV-1 infection , and we propose t...
The work described in this report is directed at how HIV-1 viral RNA populations differ between the blood plasma and male genital tract in established infection . This site is of special interest since it is the proximal source of most transmissions of HIV-1 . Thus , lessons learned about HIV-1 in the seminal tract are...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/hiv", "infection", "and", "aids", "molecular", "biology", "virology/vaccines" ]
2010
HIV-1 Populations in Semen Arise through Multiple Mechanisms
Autosomal dominant lateral temporal epilepsy ( ADTLE ) is a focal epilepsy syndrome caused by mutations in the LGI1 gene , which encodes a secreted protein . Most ADLTE-causing mutations inhibit LGI1 protein secretion , and only a few secretion-positive missense mutations have been reported . Here we describe the effec...
Temporal lobe epilepsy is the most common form of focal epilepsy . It is frequently associated with structural brain abnormalities , but genetic forms caused by mutations in major genes have also been described . Autosomal dominant lateral temporal epilepsy ( ADLTE ) is a familial condition characterized by focal seizu...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "protein", "transport", "medicine", "and", "health", "sciences", "protein", "interactions", "cell", "processes", "physiological", "processes", "mutation", "substitution", "mutation", "immunoprecipitation", "co-immunoprecipitation", "cellular", "structures", "and", "organelles...
2016
Secretion-Positive LGI1 Mutations Linked to Lateral Temporal Epilepsy Impair Binding to ADAM22 and ADAM23 Receptors
The procambium and cambium are meristematic tissues from which vascular tissue is derived . Vascular initials differentiate into phloem towards the outside of the stem and xylem towards the inside . A small peptide derived from CLV-3/ESR1-LIKE 41 ( CLE41 ) is thought to promote cell divisions in vascular meristems by s...
Plants transport water and nutrients throughout their bodies using a specialised vascular system . Vascular tissue is also responsible for providing structural support to plants; for example , wood is made up of specialised vascular cells . Consequently , the vascular system constitutes the majority of plant biomass . ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "plant", "science", "genetic", "mutation", "plant", "growth", "and", "development", "gene", "expression", "genetics", "plant", "genetics", "plant", "biology", "biology", "genetics", "and", "genomics", "gene", "function" ]
2012
Plant Vascular Cell Division Is Maintained by an Interaction between PXY and Ethylene Signalling
During the replication cycle of double-stranded ( ds ) RNA viruses , the viral RNA-dependent RNA polymerase ( RdRP ) replicates and transcribes the viral genome from within the viral capsid . How the RdRP molecules are packaged within the virion and how they function within the confines of an intact capsid are intrigui...
Viral polymerases replicate the genome of the virus , which is essential for the synthesis of the progeny . All double-stranded ( dsRNA ) viruses have virion-associated polymerases that catalyze RNA synthesis within an intact capsid . Picobirnavirus ( PBV ) is a small dsRNA virus , and it has been shown that the capsid...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "sequencing", "techniques", "nucleic", "acid", "synthesis", "dna-binding", "proteins", "microbiology", "viral", "structure", "viruses", "polymerases", "rna", "viruses", "sequence", "motif", "analysis", "molecular", "biology", "techniques", "rna", "synthesis", "microbial",...
2016
Initiation of RNA Polymerization and Polymerase Encapsidation by a Small dsRNA Virus
Biological systems can share and collectively process information to yield emergent effects , despite inherent noise in communication . While man-made systems often employ intricate structural solutions to overcome noise , the structure of many biological systems is more amorphous . It is not well understood how commun...
Biological systems must function despite inherent noise in their communication . Systems that enjoy structural stability , such as biological neural networks , could potentially overcome noise using simple redundancy-based procedures . However , when individuals have little control over who they interact with , it is u...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "collective", "animal", "behavior", "invertebrates", "ecology", "and", "environmental", "sciences", "sociology", "social", "sciences", "animals", "habits", "animal", "behavior", "deserts", "social", "communication", "zoology", "nesting", "habits", "computer", "and", "in...
2018
Limits on reliable information flows through stochastic populations
A gene expression atlas is an essential resource to quantify and understand the multiscale processes of embryogenesis in time and space . The automated reconstruction of a prototypic 4D atlas for vertebrate early embryos , using multicolor fluorescence in situ hybridization with nuclear counterstain , requires dedicate...
We propose a workflow to map the expression domains of multiple genes onto a series of 3D templates , or “atlas” , during early embryogenesis . It was applied to the zebrafish at different stages between 4 and 6 . 3 hpf , generating 6 templates . Our system overcomes the lack of significant morphological landmarks in e...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "vertebrates", "osteichthyes", "developmental", "biology", "zebrafish", "fishes", "gene", "mapping", "engineering", "and", "technology", "image", "processing", "signal", "processing", "biology", "and", "life", "sciences", "molecular", "biology", "techniques", "computation...
2014
A Digital Framework to Build, Visualize and Analyze a Gene Expression Atlas with Cellular Resolution in Zebrafish Early Embryogenesis
Drosophila melanogaster is a valuable invertebrate model for viral infection and antiviral immunity , and is a focus for studies of insect-virus coevolution . Here we use a metagenomic approach to identify more than 20 previously undetected RNA viruses and a DNA virus associated with wild D . melanogaster . These virus...
The fruit fly Drosophila melanogaster is extensively used as a model species for molecular biology and genetics . It is also widely studied for its evolutionary history , helping us understand how natural selection has shaped the genome . Drosophila research has been particularly valuable in determining how the insect ...
[ "Abstract", "Introduction", "Results", "and", "Discussion", "Materials", "and", "Methods" ]
[]
2015
The Discovery, Distribution, and Evolution of Viruses Associated with Drosophila melanogaster
The level of available iron in the mammalian host is extremely low , and pathogenic microbes must compete with host proteins such as transferrin for iron . Iron regulation of gene expression , including genes encoding iron uptake functions and virulence factors , is critical for the pathogenesis of the fungus Cryptococ...
Opportunistic fungal pathogens and other invading microbes must overcome extreme iron limitation to proliferate in the mammalian host . It is not yet known which iron sources are preferred by fungal pathogens of mammals , although the mechanisms of acquisition are beginning to be explored . Some fungi produce iron-chel...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "yeast", "and", "fungi", "cell", "biology", "infectious", "diseases", "microbiology" ]
2008
Iron Source Preference and Regulation of Iron Uptake in Cryptococcus neoformans
The utilization of symbols such as words and numbers as mental tools endows humans with unrivalled cognitive flexibility . In the number domain , a fundamental first step for the acquisition of numerical symbols is the semantic association of signs with cardinalities . We explored the primitives of such a semantic mapp...
We use symbols , such as numbers , as mental tools for abstract and precise representations . Humans share with animals a language-independent system for representing numerical quantity , but number symbols are learned during childhood . A first step in the acquisition of number symbols constitutes an association of si...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience" ]
2007
Semantic Associations between Signs and Numerical Categories in the Prefrontal Cortex
Paracoccidiodomycosis ( PCM ) is a clinically important fungal disease that can acquire serious systemic forms and is caused by the thermodimorphic fungal Paracoccidioides spp . PCM is a tropical disease that is endemic in Latin America , where up to ten million people are infected; 80% of reported cases occur in Brazi...
The fungal genus Paracoccidioides is the causal agent of paracoccidioidomycosis ( PCM ) , a neglected tropical disease that is endemic in several countries of South America . Paracoccidioides is a pathogenic dimorphic fungus that is capable of converting to a virulent yeast form after inhalation by the host . Therefore...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "fungal", "genomics", "mycology", "bioinformatics", "genome", "analysis", "fungal", "genetics", "database", "and", "informatics", "methods", "genetics", "biology", "and", "life", "sciences", "computational", "biology", "software-aided", "sequence", "analysis", "research",...
2014
Genome Update of the Dimorphic Human Pathogenic Fungi Causing Paracoccidioidomycosis
Small RNAs ( sRNAs ) are becoming increasingly recognized as important regulators in bacteria . To investigate the contribution of sRNA mediated regulation to virulence in Vibrio cholerae , we performed high throughput sequencing of cDNA generated from sRNA transcripts isolated from a strain ectopically expressing ToxT...
Vibrio cholerae is the causative agent of the diarrheal disease cholera , which remains a significant public health issue in Africa , South Asia and recently Haiti . To better understand virulence gene regulation in V . cholerae we sought to investigate the contribution of small non-coding regulatory RNAs ( sRNAs ) to ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "gram", "negative", "microbial", "pathogens", "genetics", "and", "genomics", "biology", "microbiology", "bacterial", "pathogens", "pathogenesis" ]
2011
A Genome-Wide Approach to Discovery of Small RNAs Involved in Regulation of Virulence in Vibrio cholerae
Dengue virus NS5 protein plays multiple functions in the cytoplasm of infected cells , enabling viral RNA replication and counteracting host antiviral responses . Here , we demonstrate a novel function of NS5 in the nucleus where it interferes with cellular splicing . Using global proteomic analysis of infected cells t...
Mapping host-pathogen interactions has proven fundamental for understanding how viruses manipulate host machinery and how cellular processes are regulated during infection . Dengue virus poses a major threat to public health: two-thirds of the world’s population is now at risk from infection by this mosquito-borne viru...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "viral", "transmission", "and", "infection", "gene", "regulation", "rna", "extraction", "microbiology", "alternative", "splicing", "extraction", "techniques", "research", "and", "analysis", "methods", "small", "interfering", "rnas", "genome", "complexity", "gene", "expr...
2016
The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing
Cilia have a unique diffusion barrier ( “gate” ) within their proximal region , termed transition zone ( TZ ) , that compartmentalises signalling proteins within the organelle . The TZ is known to harbour two functional modules/complexes ( Meckel syndrome [MKS] and Nephronophthisis [NPHP] ) defined by genetic interacti...
The primary cilium is a structure found in most animal cell types . Much like an antenna , it is responsible for sensing extracellular signals , including light and small molecules , and conveying this information to the receiving cell and respective tissue or organ . At the base of the cilium is the transition zone ( ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "caenorhabditis", "animals", "fibroblasts", "animal", "models", "membrane", "proteins", "caenorhabditis", "elegans", "model", "organisms", "connective", "tissue", "cells", "genetic", "interactions", "cellular", "st...
2016
MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition Zone
Most part of Southeast Asia is considered endemic for human-infecting Taenia tapeworms; Taenia solium , T . saginata , and T . asiatica . However , until now there was no report of the occurrence of human cases of T . asiatica in Lao PDR . This study , conducted in Savannakhet Province , Lao PDR , microscopically exami...
Southeast Asian Countries are endemic for several foodborne and soil-transmitted helminths occurring in different levels and areas , depending on environmental and cultural conditions . This study aimed to study the soil-transmitted helminths ( STHs ) and foodborne parasites in Savannakhet Province of Lao PDR , borderi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "lao", "people", "education", "mitochondrial", "dna", "sociology", "tropical", "diseases", "social", "sciences", "parasitic", "diseases", "ethnicities", "forms", "of", "dna", "neglected", "tropical", "diseases", "molecular", "b...
2018
Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR
Post Kala-azar Dermal Leishmaniasis ( PKDL ) is a sequel of Visceral Leishmaniasis ( VL ) . The patients act as a reservoir for the causative parasite ( i . e . Leishmania donovani ) and thus should be diagnosed and treated with the utmost urgency to prevent the transmission of the disease . In this study , we tried to...
PKDL is a sequel of VL , which acts as a source of leishmaniasis and should be diagnosed and treated at the earliest possible time to prevent disease transmission . In Bangladesh during the period from May 2016 to October 2017 , a total of 5 patients were diagnosed to have Miltefosine induced unilateral ophthalmic comp...
[ "Abstract", "Introduction", "Results/Discussion" ]
[ "kala-azar", "medicine", "and", "health", "sciences", "physicians", "medical", "doctors", "clinical", "research", "design", "medical", "personnel", "ocular", "anatomy", "tropical", "diseases", "parasitic", "diseases", "health", "care", "research", "design", "health", ...
2018
Corneal complications following Post Kala-azar Dermal Leishmaniasis treatment
Astrocytes integrate and process synaptic information and exhibit calcium ( Ca2+ ) signals in response to incoming information from neighboring synapses . The generation of Ca2+ signals is mostly attributed to Ca2+ release from internal Ca2+ stores evoked by an elevated metabotropic glutamate receptor ( mGluR ) activit...
Astrocytes are considered as active partners in neural information processing , because they integrate and process synaptic information and control synaptic transmission . Neuronal transmitter release induces the generation of Ca2+ signals in astrocytes . The functional role of astrocytic Ca2+ signals is still under de...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "neurochemistry", "action", "potentials", "nervous", "system", "astrocytes", "endoplasmic", "reticulum", "cell", "processes", "membrane", "potential", "electrophysiology", "neuroscience", "macroglial", "cells", "neurotransmitters", "...
2017
Spatial separation of two different pathways accounting for the generation of calcium signals in astrocytes
Fast-spiking ( FS ) cells in the neocortex are interconnected both by inhibitory chemical synapses and by electrical synapses , or gap-junctions . Synchronized firing of FS neurons is important in the generation of gamma oscillations , at frequencies between 30 and 80 Hz . To understand how these synaptic interactions ...
Oscillations of the electrical field in the brain at 30–80 Hz ( gamma oscillations ) reflect coordinated firing of neurons during cognitive , sensory , and motor activity , and are thought to be a key phenomenon in the organization of neural processing in the cortex . Synchronous firing of a particular type of neuron ,...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "neuroscience/neuronal", "signaling", "mechanisms", "neuroscience/theoretical", "neuroscience", "computational", "biology/computational", "neuroscience" ]
2010
Synchronization of Firing in Cortical Fast-Spiking Interneurons at Gamma Frequencies: A Phase-Resetting Analysis
JC virus is a member of the Polyomavirus family of DNA tumor viruses and the causative agent of progressive multifocal leukoencephalopathy ( PML ) . PML is a disease that occurs primarily in people who are immunocompromised and is usually fatal . As with other Polyomavirus family members , the replication of JC virus (...
Polyomaviruses have been invaluable tools for biomedical research into basic cellular processes . It is becoming increasingly clear , however , that members of this family are also involved in human diseases , particularly among the immunocompromised and the elderly . The subject of this study , the JC virus ( JCV ) , ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "biochemistry", "dna", "replication", "nucleic", "acids", "proteins", "virology", "protein", "structure", "dna", "biology", "dna-binding", "proteins", "microbiology", "viral", "replication", "biophysics" ]
2014
Insights into the Initiation of JC Virus DNA Replication Derived from the Crystal Structure of the T-Antigen Origin Binding Domain
Bothrops atrox snakes are the leading cause of snake bites in Northern Brazil . The venom of this snake is not included in the antigen pool used to obtain the Bothrops antivenom . There are discrepancies in reports on the effectiveness of this antivenom to treat victims bitten by B . atrox snakes . However , these stud...
Bothrops atrox is the dominant species responsible for accidental human snake bites in Northern Brazil . The efficacy of antivenom therapy to correct the systemic disturbances , including hemostatic disorders , caused by Brazilian Bothrops is well known . However , two fundamental issues need to be addressed in this re...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "toxins", "pathology", "and", "laboratory", "medicine", "fibrinogen", "immunology", "tropical", "diseases", "vertebrates", "animals", "toxicology", "toxic", "agents", "muscle", "regeneration", "developmental", "biology", "organism"...
2017
Experimental Bothrops atrox envenomation: Efficacy of antivenom therapy and the combination of Bothrops antivenom with dexamethasone
Intracellular Local Ca releases ( LCRs ) from sarcoplasmic reticulum ( SR ) regulate cardiac pacemaker cell function by activation of electrogenic Na/Ca exchanger ( NCX ) during diastole . Prior studies demonstrated the existence of powerful compensatory mechanisms of LCR regulation via a complex local cross-talk of Ca...
Life’s vital rhythm , the heartbeat is guaranteed by specialized , cardiac pacemaker cells . Based upon the Hodgkin-Huxley membrane excitation theory and its application to cardiac cells , the cardiac pacemaker clock has been , for a long time , to be considered essentially a surface membrane oscillator , i . e . a mem...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "biotechnology", "medicine", "and", "health", "sciences", "action", "potentials", "depolarization", "applied", "mathematics", "membrane", "potential", "electrophysiology", "neuroscience", "simulation", "and", "modeling", "algorithms", "ion", "channels", "mathematics", "pace...
2017
Stabilization of diastolic calcium signal via calcium pump regulation of complex local calcium releases and transient decay in a computational model of cardiac pacemaker cell with individual release channels
Human neutrophil antigen 2 ( HNA-2 ) deficiency is a common phenotype as 3–5% humans do not express HNA-2 . HNA-2 is coded by CD177 gene that associates with human myeloproliferative disorders . HNA-2 deficient individuals are prone to produce HNA-2 alloantibodies that cause a number of disorders including transfusion-...
Human neutrophil antigen 2 ( HNA-2 ) is coded by CD177 gene that involves in human myeloproliferative disorders . HNA-2 expression varies among humans and about 3–5% people lack HNA-2 expression . HNA-2 deficient people are susceptible to produce HNA-2 alloantibodies , which play a pathological role in various human di...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Genetic Mechanism of Human Neutrophil Antigen 2 Deficiency and Expression Variations
Natronomonas pharaonis is an archaeon adapted to two extreme conditions: high salt concentration and alkaline pH . It has become one of the model organisms for the study of extremophilic life . Here , we present a genome-scale , manually curated metabolic reconstruction for the microorganism . The reconstruction itself...
Extremophiles are organisms that thrive in physically or geochemically extreme conditions that are detrimental , even lethal , to the majority of life on Earth . Natronomonas pharaonis is one that has been able to adapt to both high salt concentration and an alkaline pH . In this study , we investigate the chemical rea...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods" ]
[ "microbiology/microbial", "physiology", "and", "metabolism", "computational", "biology/metabolic", "networks" ]
2010
Characterization of Growth and Metabolism of the Haloalkaliphile Natronomonas pharaonis
The zebrafish adult pigment pattern has emerged as a useful model for understanding the development and evolution of adult form as well as pattern-forming mechanisms more generally . In this species , a series of horizontal melanophore stripes arises during the larval-to-adult transformation , but the genetic and cellu...
Vertebrate pigment patterns are stunningly diverse and have been an important model of pattern formation for more than a century . Nevertheless , we still know remarkably little about the genes and cell behaviors that underlie the generation of specific patterns . To elucidate such mechanisms , a large number of pigmen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "model", "organisms", "genetics", "biology", "genetics", "and", "genomics" ]
2012
Melanophore Migration and Survival during Zebrafish Adult Pigment Stripe Development Require the Immunoglobulin Superfamily Adhesion Molecule Igsf11
A model of the gene-regulatory-network ( GRN ) , governing growth , survival , and differentiation of melanocytes , has emerged from studies of mouse coat color mutants and melanoma cell lines . In this model , Transcription Factor Activator Protein 2 alpha ( TFAP2A ) contributes to melanocyte development by activating...
Neural crest-derived pigment cells , known as melanocytes , are important to an organism's survival because they protect skin cells from ultraviolet radiation , camouflage the organism from predators , and contribute to sexual selection . Networks of regulatory proteins control the steps of melanocyte development , inc...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics", "developmental", "biology" ]
2010
Differentiation of Zebrafish Melanophores Depends on Transcription Factors AP2 Alpha and AP2 Epsilon
Alternative splicing is commonly used by the Metazoa to generate more than one protein from a gene . However , such diversification of the proteome by alternative splicing is much rarer in fungi . We describe here an ancient fungal alternative splicing event in which these two proteins are generated from a single alter...
The role of duplicated genes in originating new functions is an important question in evolution . Almost all species have duplicated genes that carry out similar but not identical functions . Similar proteins that perform different functions can also be generated when one gene generates multiple mRNAs by alternative sp...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "model", "organisms", "molecular", "cell", "biology", "genetics", "biology", "microbiology", "evolutionary", "biology", "genetics", "and", "genomics" ]
2013
Alternative Splicing and Subfunctionalization Generates Functional Diversity in Fungal Proteomes
The covalent attachment of adenosine monophosphate ( AMP ) to proteins , a process called AMPylation ( adenylylation ) , has recently emerged as a novel theme in microbial pathogenesis . Although several AMPylating enzymes have been characterized , the only known virulence protein with de-AMPylation activity is SidD fr...
The covalent attachment of adenosine monophosphate ( AMP ) to proteins , a process called AMPylation ( adenylylation ) , has recently emerged as a novel theme in microbial pathogenesis . While AMPylases from various pathogenic microorganisms have recently been characterized , the only virulence protein with de-AMPylati...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "cellular", "structures", "subcellular", "organelles", "enzymes", "microbiology", "bacterial", "diseases", "infectious", "diseases", "signaling", "in", "cellular", "processes", "legionella", "microbial", "pathogens", "membranes", "and", "sorting", "ras", "sign...
2013
Structural Basis for Rab1 De-AMPylation by the Legionella pneumophila Effector SidD
Singapore experiences endemic dengue , with 2013 being the largest outbreak year known to date , culminating in 22 , 170 cases . Given the limited resources available , and that vector control is the key approach for prevention in Singapore , it is important that public health professionals know where resources should ...
Dengue fever , the most prevalent mosquito-borne viral disease today , is caused by Dengue virus ( DENV ) and transmitted to human by Aedes mosquitoes , primarily the Ae . aegypti and Ae . albopictus . The key approach to mitigating dengue transmission is to control the Aedes population , and this often involve vector ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "geographical", "locations", "tropical", "diseases", "animals", "geoinformatics", "singapore", "artificial", "intelligence", "neglected", "tropical", "diseases", "infectious", "disease", "control", "insect", "vectors...
2018
Mapping dengue risk in Singapore using Random Forest
Chagas disease , caused by the unicellular parasite Trypanosoma cruzi , claims 50 , 000 lives annually and is the leading cause of infectious myocarditis in the world . As current antichagastic therapies like nifurtimox and benznidazole are highly toxic , ineffective at parasite eradication , and subject to increasing ...
Chagas disease , an infection that afflicts millions of people in Central and South America , is caused by the unicellular parasite Trypanosoma cruzi . In the chronic stage of the disease , patients' hearts are adversely affected . Chagas is the leading cause of infectious heart disease in the world . The current drugs...
[ "Abstract", "Introduction", "Methods", "Results", "and", "Discussion" ]
[ "infectious", "diseases/neglected", "tropical", "diseases", "computational", "biology/comparative", "sequence", "analysis", "biochemistry/theory", "and", "simulation", "infectious", "diseases/protozoal", "infections", "computational", "biology/molecular", "dynamics", "biochemistry/...
2010
Computational Identification of Uncharacterized Cruzain Binding Sites
Individual differences in brain functional networks may be related to complex personal identifiers , including health , age , and ability . Dynamic network theory has been used to identify properties of dynamic brain function from fMRI data , but the majority of analyses and findings remain at the level of the group . ...
Complex patterns of activity in each individual human brain generate the unique range of thoughts and behaviors that person experiences . Individual differences in ability , age , state of mind , and other characteristics are tied to differences in brain activity , but determination of the exact nature of these relatio...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "diagnostic", "radiology", "functional", "magnetic", "resonance", "imaging", "neural", "networks", "demography", "social", "sciences", "neuroscience", "learning", "and", "memory", "magnetic", "resonance", "imaging", "regression", ...
2016
Individual Differences in Dynamic Functional Brain Connectivity across the Human Lifespan
Kaposi’s sarcoma-associated herpesvirus ( KSHV ) latently infects host cells and establishes lifelong persistence as an extra-chromosomal episome in the nucleus . To persist in proliferating cells , the viral genome typically replicates once per cell cycle and is distributed into daughter cells . This process involves ...
KSHV latently infects cells and establishes lifelong persistence , but the underlying mechanisms of this process has not been fully elucidated . Here , we find a novel host protein NDRG1 is highly up-regulated by KSHV infection and the viral protein LANA is essential in this process . NDRG1 is a multiple functional pro...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "transfection", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "viral", "structure", "plasmid", "construction", "viruses", "dna", "replication", "dna", "viruses", "viral", "genome", "dna", "constructio...
2019
NDRG1 facilitates the replication and persistence of Kaposi’s sarcoma-associated herpesvirus by interacting with the DNA polymerase clamp PCNA
Natural killer ( NK ) cells are essential immune cells against several pathogens . Not much is known regarding the roll of NK cells in Orientia tsutsugamushi infection . Thus , this study aims to determine the level , function , and clinical relevance of NK cells in patients with scrub typhus . This study enrolled fift...
Orientia tsutsugamushi is an obligate intracellular bacterium . It primarily invades endothelial cells , macrophages , monocytes , and dendritic cells . Plasma concentrations of interferon ( IFN ) -γ , several cytokines and chemokines , which are known to recruit natural killer ( NK ) cells and T cells , were found to ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "flow", "cytometry", "typhus", "medicine", "and", "health", "sciences", "innate", "immune", "system", "immune", "cells", "immune", "physiology", "cytokines", "pathology", "and", "laboratory", "medicine", "body", "fluids", "immunology", "toxicology", ...
2017
Increased level and interferon-γ production of circulating natural killer cells in patients with scrub typhus
The origin of Plasmodium falciparum , the etiological agent of the most dangerous forms of human malaria , remains controversial . Although investigations of homologous parasites in African Apes are crucial to resolve this issue , studies have been restricted to a chimpanzee parasite related to P . falciparum , P . rei...
Chimpanzees and gorillas are known to have malaria parasites ( genus Plasmodium ) similar to those that infect humans . It is likely that detailed molecular studies of these parasites will help understand important aspects of the malaria disease and of immune defences in humans , and could then guide the development of...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "infectious", "diseases/protozoal", "infections", "evolutionary", "biology/microbial", "evolution", "and", "genomics", "microbiology/microbial", "evolution", "and", "genomics", "microbiology/parasitology" ]
2010
On the Diversity of Malaria Parasites in African Apes and the Origin of Plasmodium falciparum from Bonobos
Transcription , replication , and repair involve interactions of specific genomic loci with many different proteins . How these interactions are orchestrated at any given location and under changing cellular conditions is largely unknown because systematically measuring protein–DNA interactions at a specific locus in t...
DNA is packaged by proteins into a structure called chromatin . This packaging plays an important role in gene transcription , DNA replication , and DNA repair . The regulation of these processes is determined by the interplay of proteins that physically interact with specific loci . Although many chromatin proteins ha...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "sequencing", "techniques", "taxonomy", "methods", "and", "resources", "dna-binding", "proteins", "genomic", "library", "construction", "data", "management", "fungi", "dna", "replication", "molecular", "systematics", "dna", "construction", "epigenetics", "dna", "molecular...
2018
Decoding the chromatin proteome of a single genomic locus by DNA sequencing
Rodents move their whiskers to locate and identify objects . Cortical areas involved in vibrissal somatosensation and sensorimotor integration include the vibrissal area of the primary motor cortex ( vM1 ) , primary somatosensory cortex ( vS1; barrel cortex ) , and secondary somatosensory cortex ( S2 ) . We mapped loca...
The neocortex of the mammalian brain is divided into different regions that serve specific functions . These include sensory areas for vision , hearing , and touch , and motor areas for directing aspects of movement . However , the similarities and differences in local circuit organization between these areas are not w...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "neuroscience/motor", "systems", "neuroscience", "neuroscience/sensory", "systems" ]
2011
Laminar Analysis of Excitatory Local Circuits in Vibrissal Motor and Sensory Cortical Areas
Coxiella burnetii is the agent of the emerging zoonosis Q fever . This pathogen invades phagocytic and non-phagocytic cells and uses a Dot/Icm secretion system to co-opt the endocytic pathway for the biogenesis of an acidic parasitophorous vacuole where Coxiella replicates in large numbers . The study of the cell biolo...
Infectious diseases are among the major causes of mortality worldwide . Pathogens‚ invasion , colonization and persistence within their hosts depend on a tightly orchestrated cascade of events that are commonly referred to as host/pathogen interactions . These interactions are extremely diversified and every pathogen i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "gram", "negative", "microbial", "mutation", "emerging", "infectious", "diseases", "gene", "identification", "and", "analysis", "microbial", "pathogens", "molecular", "genetics", "host-pathogen", "interaction", "microbiology", "biology", "pathogenesis", "computational", "bi...
2014
Identification of OmpA, a Coxiella burnetii Protein Involved in Host Cell Invasion, by Multi-Phenotypic High-Content Screening
Copy number variants ( CNVs ) have recently been recognized as a common form of genomic variation in humans . Hundreds of CNVs can be detected in any individual genome using genomic microarrays or whole genome sequencing technology , but their phenotypic consequences are still poorly understood . Rare CNVs have been re...
Rare copy number variants ( CNVs ) are a frequent cause of neurological disorders such as mental retardation ( MR ) . However CNVs are also commonly identified in healthy individuals . It is therefore crucial for both diagnostic and research applications to be able to distinguish between disease-causing CNVs and “benig...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "genetics", "and", "genomics/genetics", "of", "disease", "genetics", "and", "genomics/bioinformatics", "genetics", "and", "genomics/medical", "genetics" ]
2010
Accurate Distinction of Pathogenic from Benign CNVs in Mental Retardation
Spinal cord injury often results in permanent functional impairment . Neural stem cells present in the adult spinal cord can be expanded in vitro and improve recovery when transplanted to the injured spinal cord , demonstrating the presence of cells that can promote regeneration but that normally fail to do so efficien...
Spinal cord injuries occur in more than 30 . 000 individuals each year worldwide and result in significant morbidity , with patients requiring long physical and medical care . The recent identification of resident stem cells in the adult spinal cord has opened up for the possibility of pharmacological manipulation of t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "cell", "biology", "neuroscience" ]
2008
Spinal Cord Injury Reveals Multilineage Differentiation of Ependymal Cells
The decreasing cost of sequencing is leading to a growing repertoire of personal genomes . However , we are lagging behind in understanding the functional consequences of the millions of variants obtained from sequencing . Global system-wide effects of variants in coding genes are particularly poorly understood . It is...
The number of personal genomes sequenced has grown rapidly over the last few years and is likely to grow further . In order to use the DNA sequence variants amongst individuals for personalized medicine , we need to understand the functional impact of these variants . Deleterious variants in genes can have a wide spect...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "systems", "biology", "genomics", "genetics", "biology", "computational", "biology", "genetics", "and", "genomics" ]
2013
Interpretation of Genomic Variants Using a Unified Biological Network Approach
We are flat-faced hominins with an external nose that protrudes from the face . This feature was derived in the genus Homo , along with facial flattening and reorientation to form a high nasal cavity . The nasal passage conditions the inhaled air in terms of temperature and humidity to match the conditions required in ...
This is the first investigation of nasal air conditioning in nonhuman hominoids based on computational fluid dynamics with digital topological models of the nasal passage made using medical imaging . Our comparative results of humans , chimpanzees , and macaques show that the inhaled air is conditioned poorly in humans...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "organismal", "evolution", "medicine", "and", "health", "sciences", "nose", "face", "hominin", "evolution", "human", "evolution", "vertebrates", "animals", "mammals", "primates", "respiratory", "system", "animal", "management", "animal", "performance", "old", "world", ...
2016
Impaired Air Conditioning within the Nasal Cavity in Flat-Faced Homo
The manual evaluation , classification and counting of biological objects demands for an enormous expenditure of time and subjective human input may be a source of error . Investigating the shape of red blood cells ( RBCs ) in microcapillary Poiseuille flow , we overcome this drawback by introducing a convolutional neu...
Artificial neural networks represent a state-of-the art technique in many branches of natural sciences due to their ability to fastly detect and categorize image features with high throughput . We use a special type of neural network , the so-called convolutional neural network ( CNN ) for the classification of human r...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "and", "discussion" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "body", "fluids", "neural", "networks", "engineering", "and", "technology", "signal", "processing", "neuroscience", "artificial", "neural", "networks", "red", "blood", "cells", "phase", "diagrams", "artificial...
2018
Classification of red blood cell shapes in flow using outlier tolerant machine learning
Bacterial effector proteins secreted into host plant cells manipulate those cells to the benefit of the pathogen , but effector-triggered immunity ( ETI ) occurs when effectors are recognized by host resistance proteins . The RPS4/RRS1 pair recognizes the Pseudomonas syringae pv . pisi effector AvrRps4 . AvrRps4 is pro...
An important component of the plant immune system relies on the detection of pathogen-derived effectors by immune receptors called resistance proteins . Bacterial pathogens inject effectors into the host cell via a dedicated secretion system to suppress defenses and to manipulate the physiology of the host cell to the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "anatomy", "viral", "transmission", "and", "infection", "plant", "cell", "biology", "brassica", "microbiology", "chloroplasts", "membrane", "proteins", "developmental", "biology", "plant", "science", "model", "organisms", "experimental", "organism", "systems", ...
2018
The bacterial type III-secreted protein AvrRps4 is a bipartite effector
FERONIA ( FER ) , a plasma membrane receptor-like kinase , is a central regulator of cell growth that integrates environmental and endogenous signals . A peptide ligand rapid alkalinization factor 1 ( RALF1 ) binds to FER and triggers a series of downstream events , including inhibition of Arabidopsis H+-ATPase 2 activ...
Receptor-like kinase FERONIA ( FER ) is an important regulator of plant growth and stress response and is activated by binding its peptide ligand , rapid alkalinization factor 1 ( RALF1 ) . However , how FER , a plasma membrane–localized receptor protein , regulates gene expression in the nucleus remains unclear . Here...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "phosphorylation", "messenger", "rna", "brassica", "green", "fluorescent", "protein", "luminescent", "proteins", "model", "organisms", "experimental", "organism", "systems", "seedlings", "plants", "arabidopsis", "thaliana", "research", "and", "analysis", "methods", "anima...
2018
EBP1 nuclear accumulation negatively feeds back on FERONIA-mediated RALF1 signaling
When a flashed stimulus is followed by a backward mask , subjects fail to perceive it unless the target-mask interval exceeds a threshold duration of about 50 ms . Models of conscious access postulate that this threshold is associated with the time needed to establish sustained activity in recurrent cortical loops , bu...
Understanding the neural mechanisms that distinguish between conscious and nonconscious processes is a crucial issue in cognitive neuroscience . In this study , we focused on the transition that causes a visual stimulus to cross the threshold to consciousness , i . e . , visibility . We used a backward masking paradigm...
[ "Abstract", "Introduction", "Results", "Discussion" ]
[ "neuroscience", "homo", "(human)" ]
2007
Brain Dynamics Underlying the Nonlinear Threshold for Access to Consciousness
Infections by Neisseria meningitidis show duality between frequent asymptomatic carriage and occasional life-threatening disease . Bacterial and host factors involved in this balance are not fully understood . Cytopathic effects and cell damage may prelude to pathogenesis of isolates belonging to hyper-invasive lineage...
Acquisition of Neisseria meningitidis often leads to asymptomatic colonization ( carriage ) and rarely results in invasive disease associated with tissue injury . The reasons that make disease-associated isolates ( pathogenic isolates ) but not asymptomatic carriage isolates able to invade the host to establish disease...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "microbiology/cellular", "microbiology", "and", "pathogenesis", "microbiology/immunity", "to", "infections" ]
2009
Differential Modulation of TNF-α–Induced Apoptosis by Neisseria meningitidis
The human filarial parasite Onchocerca volvulus is the causative agent of onchocerciasis ( river blindness ) . It causes blindness in 270 , 000 individuals with an additional 6 . 5 million suffering from severe skin pathologies . Current international control programs focus on the reduction of microfilaridermia by annu...
The human filarial parasite Onchocerca volvulus is the causative agent of onchocerciasis ( river blindness ) . To curb transmission and eventually eliminate the disease , current mass drug administration programs rely on ivermectin that primarily targets the microfilariae , which are released from adult female worms . ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "onchocerca", "volvulus", "helminths", "tropical", "diseases", "parasitic", "diseases", "animals", "onchocerca", "drug", "screening", "physiological", "processes", "developmental", "biology", "age", "groups", "negl...
2019
Development of a preliminary in vitro drug screening assay based on a newly established culturing system for pre-adult fifth-stage Onchocerca volvulus worms