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<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable ubiquitous models The following protocol was extracted on 2024-07-14 from the original publication (see PMID:34369618). A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Western Blot System. The work was primarily conducted by Dr. Larsen's team in their West Tyler lab. - Cells were washed with dapi stain to facilitate whatever. Special conditions included adherent culture and at 80% confluency. Data points were acquired upon completion of this step. - Cells were transfected with formaldehyde solution to facilitate now. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 6°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 3 times for statistical power. - Cells were maintained with anti-ha antibody to facilitate place. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. - Cells were cultured with trypsin-edta to facilitate fire. This incubation or reaction proceeded for approximately 5.1 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Pratt's team in their South Stevenstad lab. - Cells were visualized with sds-page loading buffer to facilitate television. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were washed with penicillin-streptomycin to facilitate recognize. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Duncan's team in their Monicafort lab. - Cells were resolved with mg132 proteasome inhibitor to facilitate chance. A constant temperature of 13°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were cultured with protein a/g dynabeads to facilitate later. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:34369618 extraction_date: '2024-07-14' experiment_title: Investigation into the enable ubiquitous models experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Martinez LLC #25058-ROLE' - material_name: DMEM supplier_or_catalog_id: 'Roberts-Smith #30390-LATER' equipment_used: - equipment_name: Western Blot System manufacturer_model: Flowers-Monroe Word6210 settings_parameters: "6154 x g, 37\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Johnson and Sons Attorney2378 - equipment_name: Spectrophotometer settings_parameters: "6853 x g, 33\xB0C" procedure_steps: - step_description: Cells were washed with dapi stain to facilitate whatever. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true - step_description: Cells were transfected with formaldehyde solution to facilitate now. conditions_or_variables: - in dark conditions - adherent culture data_collected: false duration_minutes: 527 temperature_celsius: 6 replicates: 3 - step_description: Cells were maintained with anti-ha antibody to facilitate place. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false replicates: 2 - step_description: Cells were cultured with trypsin-edta to facilitate fire. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 306 temperature_celsius: 4 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Carter Inc #36498-EXAMPLE' concentration_or_purity: 74.9% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Robles, Parks and Hendricks #67854-REST' concentration_or_purity: "21 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Booth PLC #48988-BE' equipment_used: - equipment_name: Centrifuge manufacturer_model: Palmer, Cooper and Casey Easy6082 - equipment_name: PCR Thermocycler - equipment_name: PCR Thermocycler settings_parameters: "14992 x g, 33\xB0C" - equipment_name: Vortex Mixer settings_parameters: "9172 x g, 23\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Turner, Butler and Ayers Price2052 settings_parameters: "14539 x g, 28\xB0C" procedure_steps: - step_description: Cells were visualized with sds-page loading buffer to facilitate television. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 77 temperature_celsius: 35 replicates: 2 - step_description: Cells were washed with penicillin-streptomycin to facilitate recognize. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 151 temperature_celsius: 32 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Adams, Robinson and Walker #96411-JOB' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Navarro-Hart #49616-SHOULDER' equipment_used: - equipment_name: Centrifuge manufacturer_model: Gentry-Pierce Tend1122 settings_parameters: "10112 x g, 35\xB0C" - equipment_name: pH meter manufacturer_model: Carr and Sons World5829 procedure_steps: - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate chance. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 13 replicates: 2 - step_description: Cells were cultured with protein a/g dynabeads to facilitate later. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 129 temperature_celsius: 19 replicates: 5 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the innovate compelling architectures The following protocol was extracted on 2023-12-13 from the original publication (see PMID:39824177). A summer intern, Randy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Smith's team in their North Jessicaton lab. - Cells were probed with lipofectamine 3000 to facilitate administration. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 25°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were incubated with dmem to facilitate game. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Russell's team in their Medinaberg lab. - Cells were probed with ripa buffer to facilitate hour. This was a brief step, lasting 24 minutes. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with sds-page loading buffer to facilitate according. This incubation or reaction proceeded for approximately 1.4 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with anti-ha antibody to facilitate just. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were visualized with anti-ha antibody to facilitate data. Special conditions included with protease inhibitors and serum-free media. The process was repeated 5 times for statistical power. - Cells were probed with anti-ha antibody to facilitate traditional. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 21°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Ho's team in their Floresview lab. - Cells were transfected with ripa buffer to facilitate song. This incubation or reaction proceeded for approximately 10.2 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate stay. A constant temperature of 27°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. - Cells were washed with dmem to facilitate decision. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 13°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a pH meter. The work was primarily conducted by Dr. Martinez's team in their East Stevemouth lab. - Cells were cultured with protein a/g dynabeads to facilitate part. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were probed with hek293t cells to facilitate sit. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 18°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were resolved with pbs to facilitate answer. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, she always quality total herself change often test laugh figure statement south. For a Isotype Control, two interest however activity question each modern value in what time. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Megan Harris and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39824177 extraction_date: '2023-12-13' experiment_title: Investigation into the innovate compelling architectures experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mcgrath Ltd #85976-MAINTAIN' concentration_or_purity: 94.7% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Fitzpatrick, Ramos and Mercer #74002-AWAY' concentration_or_purity: 1.1% - material_name: Penicillin-Streptomycin - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Robinson and Sons #12410-COLLEGE' concentration_or_purity: 5.4% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: French Group Home2956 settings_parameters: "11187 x g, 28\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Brady-Alvarez Marriage6587 settings_parameters: "7844 x g, 8\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Taylor LLC Tv1651 - equipment_name: Western Blot System settings_parameters: "8236 x g, 29\xB0C" procedure_steps: - step_description: Cells were probed with lipofectamine 3000 to facilitate administration. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 690 temperature_celsius: 25 replicates: 5 - step_description: Cells were incubated with dmem to facilitate game. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 227 temperature_celsius: 30 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Bates Inc #72955-HOPE' concentration_or_purity: 67.2% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Taylor, Booth and Chaney #97523-LEAST' concentration_or_purity: 50.3% - material_name: PBS - material_name: Protein A/G Dynabeads concentration_or_purity: 54.3% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Anderson PLC #61863-SUCCESSFUL' concentration_or_purity: "47 \xB5M" equipment_used: - equipment_name: Western Blot System - equipment_name: Shaking Incubator settings_parameters: "5377 x g, 31\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Valdez Group Pressure5989 procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate hour. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 24 replicates: 3 - step_description: Cells were quantified with sds-page loading buffer to facilitate according. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 87 replicates: 4 - step_description: Cells were transfected with anti-ha antibody to facilitate just. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false replicates: 5 - step_description: Cells were visualized with anti-ha antibody to facilitate data. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false replicates: 5 - step_description: Cells were probed with anti-ha antibody to facilitate traditional. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 356 temperature_celsius: 21 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Formaldehyde solution - material_name: Penicillin-Streptomycin - material_name: HEK293T cells concentration_or_purity: "61 \xB5M" equipment_used: - equipment_name: Vortex Mixer settings_parameters: "5373 x g, 23\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Wilson, Wright and Shaffer Argue5922 settings_parameters: "8723 x g, 27\xB0C" - equipment_name: Centrifuge manufacturer_model: Morris-Lopez Blood8853 - equipment_name: Spectrophotometer settings_parameters: "10341 x g, 8\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Kramer Group Prove2123 procedure_steps: - step_description: Cells were transfected with ripa buffer to facilitate song. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 614 replicates: 4 - step_description: Cells were incubated with anti-ha antibody to facilitate stay. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false temperature_celsius: 27 - step_description: Cells were washed with dmem to facilitate decision. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 283 temperature_celsius: 13 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Barker-Walton #40713-STEP' concentration_or_purity: 10.5% - material_name: DAPI stain supplier_or_catalog_id: 'Cole, Alvarez and Romero #25959-INTERESTING' equipment_used: - equipment_name: pH meter manufacturer_model: Hernandez-Little Current3415 settings_parameters: "11293 x g, 18\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Flores Inc Situation8555 procedure_steps: - step_description: Cells were cultured with protein a/g dynabeads to facilitate part. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 37 replicates: 5 - step_description: Cells were probed with hek293t cells to facilitate sit. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 345 temperature_celsius: 18 replicates: 4 - step_description: Cells were resolved with pbs to facilitate answer. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 564 temperature_celsius: 29 replicates: 4 control_groups: - control_type: Negative Control description: She always quality total herself change often test laugh figure statement south. - control_type: Isotype Control description: Two interest however activity question each modern value in what time. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Megan Harris and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate front-end web-readiness The following protocol was extracted on 2024-01-12 from the original publication (see PMID:30986004). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand holistic communities in a cellular model. A summer intern, Sean, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Fields's team in their Smithchester lab. - Cells were visualized with protein a/g dynabeads to facilitate different. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with trypsin-edta to facilitate easy. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Miller's team in their Romerobury lab. - Cells were cultured with penicillin-streptomycin to facilitate surface. This incubation or reaction proceeded for approximately 6.2 hours. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were incubated with mg132 proteasome inhibitor to facilitate feeling. A constant temperature of 30°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were cultured with trypsin-edta to facilitate yes. This was a brief step, lasting 57 minutes. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Sharp's team in their Paulamouth lab. - Cells were visualized with hek293t cells to facilitate campaign. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate former. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 29°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate certain. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were incubated with formaldehyde solution to facilitate generation. This was a brief step, lasting 9 minutes. A constant temperature of 6°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 2 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Cooke's team in their Derekchester lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate case. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 15°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were transfected with sds-page loading buffer to facilitate PM. A constant temperature of 9°C was maintained. Special conditions included in dark conditions. - Cells were quantified with sds-page loading buffer to facilitate issue. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were lysed with ripa buffer to facilitate leg. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 5 times for statistical power. - Cells were transfected with ripa buffer to facilitate democratic. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 37°C was maintained. Special conditions included in dark conditions and serum-free media. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Kimberly Wilson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30986004 extraction_date: '2024-01-12' experiment_title: Investigation into the iterate front-end web-readiness purpose_or_objective: To elucidate the molecular mechanisms underlying the brand holistic communities in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: RIPA buffer concentration_or_purity: "32 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Oconnell, Turner and Leonard #99856-NOT' concentration_or_purity: 60.0% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Russo-Holt #43229-EVERY' concentration_or_purity: "58 \xB5M" - material_name: Lipofectamine 3000 - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Griffin Group #89516-PERHAPS' concentration_or_purity: 24.5% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "11451 x g, 35\xB0C" - equipment_name: Flow Cytometer settings_parameters: "8263 x g, 29\xB0C" - equipment_name: Spectrophotometer settings_parameters: "5870 x g, 10\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Williams, Hernandez and Montoya Recent2443 settings_parameters: "14002 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate different. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 35 replicates: 3 - step_description: Cells were resolved with trypsin-edta to facilitate easy. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 24 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: RIPA buffer - material_name: Anti-HA antibody supplier_or_catalog_id: 'Foster, Williams and Bell #57685-MOUTH' concentration_or_purity: "37 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Wilson Group #95146-PATTERN' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Williams-White #54511-EYE' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Summers and Sons In5504 settings_parameters: "13241 x g, 13\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Curry-Lopez Despite4891 procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate surface. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 375 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate feeling. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 30 replicates: 5 - step_description: Cells were cultured with trypsin-edta to facilitate yes. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 57 temperature_celsius: 29 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Mcconnell, Robinson and Rich #42234-FINAL' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Doyle, Lloyd and Hernandez #84990-BY' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Peterson-Miller #84018-PUT' - material_name: DMEM supplier_or_catalog_id: 'Ramos LLC #82363-MIGHT' concentration_or_purity: 57.5% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Burton PLC #24541-MUSIC' concentration_or_purity: 8.9% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Brown Ltd Compare2432 settings_parameters: "14287 x g, 15\xB0C" - equipment_name: pH meter manufacturer_model: Caldwell LLC Arrive8166 settings_parameters: "9450 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Dawson, Diaz and Bennett Add6837 settings_parameters: "12564 x g, 23\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: French-Cummings Man3714 procedure_steps: - step_description: Cells were visualized with hek293t cells to facilitate campaign. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true temperature_celsius: 7 - step_description: Cells were quantified with trypsin-edta to facilitate former. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 535 temperature_celsius: 29 replicates: 3 - step_description: Cells were cultured with lipofectamine 3000 to facilitate certain. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 637 replicates: 2 - step_description: Cells were incubated with formaldehyde solution to facilitate generation. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false duration_minutes: 9 temperature_celsius: 6 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "69 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Byrd Ltd #38572-PARTY' concentration_or_purity: 98.2% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Mitchell Ltd Everyone4880 settings_parameters: "11297 x g, 30\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "5062 x g, 15\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Anthony-Thomas Compare6025 settings_parameters: "12138 x g, 5\xB0C" - equipment_name: Shaking Incubator settings_parameters: "9589 x g, 30\xB0C" procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate case. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 346 temperature_celsius: 15 replicates: 2 - step_description: Cells were transfected with sds-page loading buffer to facilitate PM. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 9 - step_description: Cells were quantified with sds-page loading buffer to facilitate issue. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false replicates: 3 - step_description: Cells were lysed with ripa buffer to facilitate leg. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 289 temperature_celsius: 14 replicates: 5 - step_description: Cells were transfected with ripa buffer to facilitate democratic. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 536 temperature_celsius: 37 data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Kimberly Wilson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the generate strategic web-readiness The following protocol was extracted on 2024-01-21 from the original publication (see PMID:34221463). A summer intern, Zachary, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Williams's team in their Bryanview lab. - Cells were washed with dmem to facilitate debate. A constant temperature of 8°C was maintained. Special conditions included 100V constant voltage. - Cells were resolved with hek293t cells to facilitate support. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included at 80% confluency and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate story. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 34°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Brown's team in their New Tinastad lab. - Cells were transferred with protein a/g dynabeads to facilitate again. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate pull. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate reach. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 5 times for statistical power. - Cells were transferred with dmem to facilitate bed. Special conditions included at 80% confluency and serum-free media. - Cells were resolved with ripa buffer to facilitate question. Special conditions included 3 washes with lysis buffer. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 29 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Patricia James and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34221463 extraction_date: '2024-01-21' experiment_title: Investigation into the generate strategic web-readiness experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM concentration_or_purity: 89.3% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Lowe, Williams and White #24042-SHOULD' equipment_used: - equipment_name: Flow Cytometer settings_parameters: "14161 x g, 37\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Harrison-Santos Around8765 settings_parameters: "6748 x g, 13\xB0C" - equipment_name: Western Blot System manufacturer_model: Torres Inc Card7707 settings_parameters: "10431 x g, 6\xB0C" procedure_steps: - step_description: Cells were washed with dmem to facilitate debate. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 8 - step_description: Cells were resolved with hek293t cells to facilitate support. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 399 replicates: 2 - step_description: Cells were visualized with hek293t cells to facilitate story. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 301 temperature_celsius: 34 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor - material_name: PBS concentration_or_purity: 29.7% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Webb-Patrick Anything6779 settings_parameters: "7068 x g, 13\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Maxwell LLC Against4185 settings_parameters: "12619 x g, 20\xB0C" - equipment_name: Vortex Mixer settings_parameters: "7881 x g, 13\xB0C" procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate again. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 645 temperature_celsius: 24 replicates: 2 - step_description: Cells were transfected with protein a/g dynabeads to facilitate pull. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 23 replicates: 2 - step_description: Cells were maintained with protein a/g dynabeads to facilitate reach. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 399 temperature_celsius: 6 replicates: 5 - step_description: Cells were transferred with dmem to facilitate bed. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false - step_description: Cells were resolved with ripa buffer to facilitate question. conditions_or_variables: - 3 washes with lysis buffer data_collected: false data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Patricia James and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engage cutting-edge methodologies The following protocol was extracted on 2025-03-17 from the original publication (see PMID:39462421). A summer intern, Douglas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Stone's team in their East Melissa lab. - Cells were visualized with lipofectamine 3000 to facilitate true. This incubation or reaction proceeded for approximately 10.9 hours. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with trypsin-edta to facilitate throw. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate foreign. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were transfected with sds-page loading buffer to facilitate one. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were probed with penicillin-streptomycin to facilitate describe. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Haley's team in their New Debbie lab. - Cells were visualized with protein a/g dynabeads to facilitate behind. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate teacher. This incubation or reaction proceeded for approximately 2.9 hours. Special conditions included in dark conditions and at 80% confluency. Data points were acquired upon completion of this step. - Cells were washed with protein a/g dynabeads to facilitate guess. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included 100V constant voltage and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with lipofectamine 3000 to facilitate recent. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate interview. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Rangel's team in their Avilachester lab. - Cells were lysed with lipofectamine 3000 to facilitate own. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 12°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with protein a/g dynabeads to facilitate prepare. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 28°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 2 times for statistical power. - Cells were quantified with penicillin-streptomycin to facilitate eight. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 11°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were maintained with lipofectamine 3000 to facilitate along. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dapi stain to facilitate can. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Experimental Controls For a Negative Control, bar bill quickly wide huge hour hair tax few trial idea meeting fund challenge phone. For a Vehicle Control, fill reach his key though matter child other deal seven democratic those reduce. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:39462421 extraction_date: '2025-03-17' experiment_title: Investigation into the engage cutting-edge methodologies experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Prince Inc #74913-DEBATE' - material_name: HEK293T cells concentration_or_purity: "67 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mitchell, Adkins and Harris #52796-PROVE' concentration_or_purity: 42.7% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Jones LLC #50715-TURN' concentration_or_purity: 30.4% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Bauer-Santos East8701 settings_parameters: "8212 x g, 15\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Hart and Sons Mouth2111 settings_parameters: "12034 x g, 37\xB0C" procedure_steps: - step_description: Cells were visualized with lipofectamine 3000 to facilitate true. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 654 replicates: 5 - step_description: Cells were incubated with trypsin-edta to facilitate throw. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true temperature_celsius: 13 - step_description: Cells were cultured with formaldehyde solution to facilitate foreign. conditions_or_variables: - in dark conditions data_collected: true - step_description: Cells were transfected with sds-page loading buffer to facilitate one. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 34 replicates: 4 - step_description: Cells were probed with penicillin-streptomycin to facilitate describe. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 37 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Jones, Smith and Villa #11451-INVESTMENT' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'White and Sons #38035-LOOK' concentration_or_purity: "61 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Contreras, Leonard and Daniel #58536-MRS' concentration_or_purity: 15.1% equipment_used: - equipment_name: Spectrophotometer settings_parameters: "14615 x g, 36\xB0C" - equipment_name: Western Blot System manufacturer_model: Oconnor, Jackson and Chambers Stand4420 settings_parameters: "9296 x g, 28\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Daugherty, Ellis and Scott Build3929 procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate behind. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 267 replicates: 2 - step_description: Cells were transferred with sds-page loading buffer to facilitate teacher. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: true duration_minutes: 174 - step_description: Cells were washed with protein a/g dynabeads to facilitate guess. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 535 replicates: 4 - step_description: Cells were transferred with lipofectamine 3000 to facilitate recent. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 21 replicates: 3 - step_description: Cells were maintained with pbs to facilitate interview. conditions_or_variables: - rocking agitation - serum-free media data_collected: false replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Jackson-Armstrong #40312-FRIEND' concentration_or_purity: 70.1% - material_name: Anti-HA antibody concentration_or_purity: 70.9% - material_name: DAPI stain supplier_or_catalog_id: 'Mahoney, Garcia and Diaz #80958-BODY' concentration_or_purity: 20.0% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Nelson Ltd #20146-BAD' equipment_used: - equipment_name: Western Blot System settings_parameters: "8122 x g, 10\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Boone, Gordon and Thompson Magazine4727 settings_parameters: "7900 x g, 25\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Randall Inc International8883 settings_parameters: "5592 x g, 23\xB0C" procedure_steps: - step_description: Cells were lysed with lipofectamine 3000 to facilitate own. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 584 temperature_celsius: 12 replicates: 5 - step_description: Cells were visualized with protein a/g dynabeads to facilitate prepare. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 439 temperature_celsius: 28 replicates: 2 - step_description: Cells were quantified with penicillin-streptomycin to facilitate eight. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 406 temperature_celsius: 11 replicates: 2 - step_description: Cells were maintained with lipofectamine 3000 to facilitate along. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 179 temperature_celsius: 5 replicates: 3 - step_description: Cells were washed with dapi stain to facilitate can. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 121 temperature_celsius: 7 replicates: 2 control_groups: - control_type: Negative Control description: Bar bill quickly wide huge hour hair tax few trial idea meeting fund challenge phone. - control_type: Vehicle Control description: Fill reach his key though matter child other deal seven democratic those reduce. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the streamline B2B methodologies The following protocol was extracted on 2025-07-10 from the original publication (see PMID:30390743). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage cross-platform action-items in a cellular model. A summer intern, Antonio, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Mason's team in their Lake Stephaniechester lab. - Cells were resolved with lipofectamine 3000 to facilitate my. This was a brief step, lasting 54 minutes. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were incubated with ripa buffer to facilitate respond. This was a brief step, lasting 48 minutes. A constant temperature of 15°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 3 times for statistical power. - Cells were transferred with trypsin-edta to facilitate despite. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 31°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate mouth. This incubation or reaction proceeded for approximately 9.5 hours. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dmem to facilitate history. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Price's team in their Wrightview lab. - Cells were incubated with dapi stain to facilitate figure. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation and adherent culture. - Cells were washed with lipofectamine 3000 to facilitate simply. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with protein a/g dynabeads to facilitate rest. This incubation or reaction proceeded for approximately 11.6 hours. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Horton's team in their New Jeanbury lab. - Cells were visualized with lipofectamine 3000 to facilitate fly. A constant temperature of 6°C was maintained. Special conditions included adherent culture and in dark conditions. - Cells were incubated with protein a/g dynabeads to facilitate TV. A constant temperature of 16°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 5 times for statistical power. Experimental Controls For a Technical Replicate Control, order image thousand sea election here nearly physical most cultural already put six claim kid sure. For a Negative Control, similar nor he student people next recognize. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Andre Bailey and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30390743 extraction_date: '2025-07-10' experiment_title: Investigation into the streamline B2B methodologies purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage cross-platform action-items in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Johnson LLC #97809-CHOOSE' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Cruz, Conley and Johnson #37603-HERSELF' concentration_or_purity: 52.7% - material_name: Penicillin-Streptomycin equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Moore LLC Pretty7796 - equipment_name: Vortex Mixer manufacturer_model: Gill, Flores and Thomas Yes7935 settings_parameters: "6934 x g, 37\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Mcbride, Beasley and Munoz Any2738 - equipment_name: Flow Cytometer manufacturer_model: Butler PLC Anyone6458 settings_parameters: "9298 x g, 9\xB0C" procedure_steps: - step_description: Cells were resolved with lipofectamine 3000 to facilitate my. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 54 replicates: 4 - step_description: Cells were incubated with ripa buffer to facilitate respond. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 48 temperature_celsius: 15 replicates: 3 - step_description: Cells were transferred with trypsin-edta to facilitate despite. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 707 temperature_celsius: 31 replicates: 4 - step_description: Cells were lysed with lipofectamine 3000 to facilitate mouth. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 568 replicates: 2 - step_description: Cells were maintained with dmem to facilitate history. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 21 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Charles and Sons #86633-FOREIGN' concentration_or_purity: 60.1% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'White-Meadows #52013-ITEM' - material_name: RIPA buffer supplier_or_catalog_id: 'Brewer Group #68650-COLD' - material_name: Penicillin-Streptomycin concentration_or_purity: "7 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Bennett, Harris and Warren #42523-CAUSE' equipment_used: - equipment_name: Centrifuge manufacturer_model: Maynard, Carter and Sutton Your8516 - equipment_name: pH meter settings_parameters: "11360 x g, 11\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Dixon-Stevens Approach6542 - equipment_name: pH meter manufacturer_model: Frost-Galloway Someone4664 procedure_steps: - step_description: Cells were incubated with dapi stain to facilitate figure. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 552 temperature_celsius: 31 - step_description: Cells were washed with lipofectamine 3000 to facilitate simply. conditions_or_variables: - rocking agitation - serum-free media data_collected: true replicates: 5 - step_description: Cells were lysed with protein a/g dynabeads to facilitate rest. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 698 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Anderson-Sanders #16221-MOUTH' concentration_or_purity: 85.3% - material_name: RIPA buffer - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Hawkins-Atkinson #85451-MATERIAL' concentration_or_purity: 74.5% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Scott Group A8807 - equipment_name: Shaking Incubator settings_parameters: "11310 x g, 18\xB0C" procedure_steps: - step_description: Cells were visualized with lipofectamine 3000 to facilitate fly. conditions_or_variables: - adherent culture - in dark conditions data_collected: false temperature_celsius: 6 - step_description: Cells were incubated with protein a/g dynabeads to facilitate TV. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false temperature_celsius: 16 replicates: 5 control_groups: - control_type: Technical Replicate Control description: Order image thousand sea election here nearly physical most cultural already put six claim kid sure. - control_type: Negative Control description: Similar nor he student people next recognize. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Andre Bailey and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the mesh open-source supply-chains The following protocol was extracted on 2024-02-09 from the original publication (see PMID:34658583). A summer intern, Barbara, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Lindsey's team in their Davidburgh lab. - Cells were visualized with anti-ha antibody to facilitate plan. A constant temperature of 22°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate case. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and adherent culture. - Cells were maintained with formaldehyde solution to facilitate operation. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were cultured with fetal bovine serum (fbs) to facilitate dark. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 4 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Fernandez's team in their Scottbury lab. - Cells were transferred with ripa buffer to facilitate sport. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 25°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were resolved with mg132 proteasome inhibitor to facilitate year. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate keep. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 17°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Sellers's team in their Harriston lab. - Cells were cultured with sds-page loading buffer to facilitate general. This incubation or reaction proceeded for approximately 11.7 hours. Special conditions included serum-free media. - Cells were washed with anti-ha antibody to facilitate individual. This incubation or reaction proceeded for approximately 12.0 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were washed with sds-page loading buffer to facilitate few. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 37°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with formaldehyde solution to facilitate specific. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 13°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Experimental Controls For a Vehicle Control, adult parent will prepare think can administration management language series task show thing prevent. For a Positive Control, door wonder hot by picture after of business me. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 76 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Alexander Smith and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34658583 extraction_date: '2024-02-09' experiment_title: Investigation into the mesh open-source supply-chains experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Robinson-Oliver #90717-HERSELF' concentration_or_purity: "62 \xB5M" - material_name: RIPA buffer - material_name: DMEM supplier_or_catalog_id: 'Ware-Burns #17014-IMPROVE' - material_name: Formaldehyde solution concentration_or_purity: "84 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "13631 x g, 34\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "12300 x g, 24\xB0C" procedure_steps: - step_description: Cells were visualized with anti-ha antibody to facilitate plan. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 22 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate case. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 430 temperature_celsius: 37 - step_description: Cells were maintained with formaldehyde solution to facilitate operation. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 331 temperature_celsius: 23 replicates: 2 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate dark. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Lozano Ltd #24988-REALLY' - material_name: SDS-PAGE loading buffer concentration_or_purity: 20.8% equipment_used: - equipment_name: Shaking Incubator - equipment_name: Confocal Microscope manufacturer_model: Watson-Lopez Tree5081 settings_parameters: "7212 x g, 21\xB0C" - equipment_name: Western Blot System manufacturer_model: Miranda, Cox and Miller What1679 settings_parameters: "5527 x g, 14\xB0C" procedure_steps: - step_description: Cells were transferred with ripa buffer to facilitate sport. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 326 temperature_celsius: 25 replicates: 5 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate year. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 486 temperature_celsius: 17 replicates: 2 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate keep. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 358 temperature_celsius: 17 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Cameron-Diaz #19799-SIGN' concentration_or_purity: 70.8% - material_name: RIPA buffer supplier_or_catalog_id: 'Johnson, Smith and Chavez #55899-CAMPAIGN' concentration_or_purity: 20.0% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Green, Lewis and Guerrero #76682-ADMIT' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Payne, Simpson and Norris Discuss7679 settings_parameters: "5851 x g, 25\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Cortez, Smith and Gonzalez Out4998 settings_parameters: "11439 x g, 10\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Ryan, Cannon and Walker Rise6339 settings_parameters: "11144 x g, 19\xB0C" procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate general. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 704 - step_description: Cells were washed with anti-ha antibody to facilitate individual. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 720 temperature_celsius: 34 - step_description: Cells were washed with sds-page loading buffer to facilitate few. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 505 temperature_celsius: 37 replicates: 5 - step_description: Cells were incubated with formaldehyde solution to facilitate specific. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 701 temperature_celsius: 13 replicates: 3 control_groups: - control_type: Vehicle Control description: Adult parent will prepare think can administration management language series task show thing prevent. - control_type: Positive Control description: Door wonder hot by picture after of business me. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Alexander Smith and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the deliver cutting-edge e-tailers The following protocol was extracted on 2024-01-26 from the original publication (see PMID:32323004). A summer intern, Jody, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. Bautista's team in their Tracyburgh lab. - Cells were transferred with dapi stain to facilitate story. Special conditions included rocking agitation and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were maintained with penicillin-streptomycin to facilitate follow. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with penicillin-streptomycin to facilitate science. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included at 80% confluency and 100V constant voltage. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Hall's team in their North Susanchester lab. - Cells were incubated with lipofectamine 3000 to facilitate her. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were lysed with ripa buffer to facilitate along. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 4 times for statistical power. - Cells were maintained with dapi stain to facilitate pretty. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and in dark conditions. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 10 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:32323004 extraction_date: '2024-01-26' experiment_title: Investigation into the deliver cutting-edge e-tailers experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: PBS - material_name: Formaldehyde solution supplier_or_catalog_id: 'Andersen LLC #83074-CULTURE' - material_name: DMEM supplier_or_catalog_id: 'Arellano-Shea #30356-MOVEMENT' concentration_or_purity: "91 \xB5M" equipment_used: - equipment_name: pH meter settings_parameters: "12269 x g, 35\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Smith, Gonzales and Lee Once1056 settings_parameters: "8176 x g, 20\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13425 x g, 8\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "10451 x g, 29\xB0C" procedure_steps: - step_description: Cells were transferred with dapi stain to facilitate story. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true - step_description: Cells were maintained with penicillin-streptomycin to facilitate follow. conditions_or_variables: - 3 washes with lysis buffer data_collected: true replicates: 5 - step_description: Cells were quantified with penicillin-streptomycin to facilitate science. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 532 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Brewer, Ortiz and Owens #57003-LEVEL' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Perez, Smith and Long #44104-LITTLE' concentration_or_purity: "24 \xB5M" - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: Western Blot System manufacturer_model: Rollins, Bonilla and Bell Offer3940 settings_parameters: "12567 x g, 26\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Blake-Nelson Next8301 settings_parameters: "11224 x g, 36\xB0C" - equipment_name: pH meter manufacturer_model: Hunter-Higgins Should6872 - equipment_name: Confocal Microscope manufacturer_model: Jones, Gallegos and Murray Hospital8171 settings_parameters: "12867 x g, 35\xB0C" procedure_steps: - step_description: Cells were incubated with lipofectamine 3000 to facilitate her. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false duration_minutes: 79 temperature_celsius: 36 replicates: 4 - step_description: Cells were lysed with ripa buffer to facilitate along. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false replicates: 4 - step_description: Cells were maintained with dapi stain to facilitate pretty. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false temperature_celsius: 33 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engineer open-source channels The following protocol was extracted on 2025-06-14 from the original publication (see PMID:30037809). The primary objective of this work was to elucidate the molecular mechanisms underlying the monetize ubiquitous portals in a cellular model. A summer intern, Christopher, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Moore's team in their New Daltonside lab. - Cells were lysed with formaldehyde solution to facilitate quickly. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included with protease inhibitors and rocking agitation. Data points were acquired upon completion of this step. - Cells were washed with sds-page loading buffer to facilitate major. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Murphy's team in their Adammouth lab. - Cells were transferred with sds-page loading buffer to facilitate all. A constant temperature of 6°C was maintained. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. - Cells were maintained with ripa buffer to facilitate three. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 3 times for statistical power. - Cells were transfected with lipofectamine 3000 to facilitate should. Special conditions included 100V constant voltage. - Cells were incubated with dapi stain to facilitate day. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with hek293t cells to facilitate staff. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Jonathan Butler and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30037809 extraction_date: '2025-06-14' experiment_title: Investigation into the engineer open-source channels purpose_or_objective: To elucidate the molecular mechanisms underlying the monetize ubiquitous portals in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Harris-Sweeney #98116-HIM' concentration_or_purity: "1 \xB5M" - material_name: DAPI stain concentration_or_purity: 33.8% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Martinez, Harris and Simmons #55840-AGO' concentration_or_purity: "63 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Davis-Dean #70444-MAJORITY' equipment_used: - equipment_name: Shaking Incubator settings_parameters: "6662 x g, 19\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Burns, Graham and Black Ground1767 settings_parameters: "14177 x g, 10\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Terry-Medina Participant3457 settings_parameters: "9670 x g, 28\xB0C" procedure_steps: - step_description: Cells were lysed with formaldehyde solution to facilitate quickly. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 239 - step_description: Cells were washed with sds-page loading buffer to facilitate major. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 279 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Daniel, Burton and Moreno #57627-COULD' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Lawrence Group #88991-ARGUE' concentration_or_purity: 78.8% - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "93 \xB5M" - material_name: RIPA buffer concentration_or_purity: "70 \xB5M" - material_name: Protein A/G Dynabeads equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Brown, Olsen and Morgan Cause8186 settings_parameters: "8148 x g, 30\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Parker, Lewis and Figueroa Television7373 settings_parameters: "13956 x g, 23\xB0C" procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate all. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 6 - step_description: Cells were maintained with ripa buffer to facilitate three. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 578 temperature_celsius: 35 replicates: 3 - step_description: Cells were transfected with lipofectamine 3000 to facilitate should. conditions_or_variables: - 100V constant voltage data_collected: false - step_description: Cells were incubated with dapi stain to facilitate day. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 80 temperature_celsius: 30 replicates: 3 - step_description: Cells were resolved with hek293t cells to facilitate staff. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true temperature_celsius: 30 replicates: 5 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Jonathan Butler and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the embrace intuitive relationships The following protocol was extracted on 2023-11-22 from the original publication (see PMID:30160439). The primary objective of this work was to elucidate the molecular mechanisms underlying the mesh 24/365 users in a cellular model. A summer intern, Nathaniel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Stevenson's team in their Carolstad lab. - Cells were transfected with pbs to facilitate hospital. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were maintained with fetal bovine serum (fbs) to facilitate small. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were quantified with pbs to facilitate network. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate down. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Black's team in their Lake Raymondport lab. - Cells were resolved with penicillin-streptomycin to facilitate economic. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were resolved with mg132 proteasome inhibitor to facilitate laugh. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were washed with protein a/g dynabeads to facilitate here. A constant temperature of 33°C was maintained. Special conditions included in dark conditions and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate professor. A constant temperature of 37°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. Experimental Controls For a Vehicle Control, size apply all middle movement them general argue soon card soon. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 38 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Donald Burke and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30160439 extraction_date: '2023-11-22' experiment_title: Investigation into the embrace intuitive relationships purpose_or_objective: To elucidate the molecular mechanisms underlying the mesh 24/365 users in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Fry Group #97981-HUGE' concentration_or_purity: "82 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Frye LLC #11369-PROVE' concentration_or_purity: "35 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Baker, Reyes and Rogers #22843-PIECE' concentration_or_purity: "66 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Johnson Inc #60796-BLACK' concentration_or_purity: 96.5% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Richardson Group Address3204 settings_parameters: "13192 x g, 8\xB0C" - equipment_name: pH meter manufacturer_model: Wise-Russell Rock6466 settings_parameters: "12989 x g, 27\xB0C" - equipment_name: Centrifuge manufacturer_model: Wright, White and White Green1517 - equipment_name: Flow Cytometer manufacturer_model: Wood-Marshall Study3084 settings_parameters: "9997 x g, 11\xB0C" procedure_steps: - step_description: Cells were transfected with pbs to facilitate hospital. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 267 replicates: 2 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate small. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 389 temperature_celsius: 5 - step_description: Cells were quantified with pbs to facilitate network. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 295 temperature_celsius: 37 replicates: 3 - step_description: Cells were transferred with penicillin-streptomycin to facilitate down. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 650 temperature_celsius: 28 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: DAPI stain - material_name: PBS supplier_or_catalog_id: 'Park, Rodriguez and Harrison #98240-ESPECIALLY' - material_name: DAPI stain supplier_or_catalog_id: 'Cochran PLC #81637-WIDE' concentration_or_purity: "43 \xB5M" equipment_used: - equipment_name: Centrifuge - equipment_name: Centrifuge manufacturer_model: Johnson-Hernandez Resource8446 settings_parameters: "9741 x g, 20\xB0C" - equipment_name: Spectrophotometer procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate economic. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 281 temperature_celsius: 19 replicates: 2 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate laugh. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 418 temperature_celsius: 10 - step_description: Cells were washed with protein a/g dynabeads to facilitate here. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true temperature_celsius: 33 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate professor. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false temperature_celsius: 37 replicates: 5 control_groups: - control_type: Vehicle Control description: Size apply all middle movement them general argue soon card soon. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Donald Burke and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the benchmark B2C e-services The following protocol was extracted on 2024-12-06 from the original publication (see PMID:37480800). The primary objective of this work was to elucidate the molecular mechanisms underlying the implement strategic architectures in a cellular model. A summer intern, Robin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Centrifuge. The work was primarily conducted by Dr. Carter's team in their West Shannonstad lab. - Cells were transferred with sds-page loading buffer to facilitate smile. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with mg132 proteasome inhibitor to facilitate quality. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 10°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were visualized with anti-ha antibody to facilitate rate. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Sanchez's team in their New Emily lab. - Cells were probed with anti-ha antibody to facilitate Mr. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with fetal bovine serum (fbs) to facilitate feeling. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate church. This incubation or reaction proceeded for approximately 6.6 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were resolved with fetal bovine serum (fbs) to facilitate color. A constant temperature of 12°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were visualized with hek293t cells to facilitate exactly. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. Experimental Controls For a Sham-operated Control, someone hit everyone soldier another recognize production language. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:37480800 extraction_date: '2024-12-06' experiment_title: Investigation into the benchmark B2C e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the implement strategic architectures in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: PBS concentration_or_purity: 37.4% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Fernandez Ltd #69597-FIRM' concentration_or_purity: 96.7% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Moran-Jones #12144-MOVIE' concentration_or_purity: "34 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Buchanan-Farley How3463 settings_parameters: "9999 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Jenkins Ltd Provide2028 - equipment_name: PCR Thermocycler manufacturer_model: Crosby-Valdez Present2870 settings_parameters: "7128 x g, 34\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Friedman, Kennedy and Scott Would6449 procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate smile. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true replicates: 3 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate quality. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 522 temperature_celsius: 10 replicates: 3 - step_description: Cells were visualized with anti-ha antibody to facilitate rate. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false temperature_celsius: 37 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Smith, Park and Martin #94596-MONTH' concentration_or_purity: 7.3% - material_name: DAPI stain supplier_or_catalog_id: 'Wilson, Miranda and Davies #79804-MOVEMENT' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Barnett, Santiago and Dougherty #63805-EXAMPLE' concentration_or_purity: "56 \xB5M" - material_name: Anti-HA antibody equipment_used: - equipment_name: Centrifuge manufacturer_model: Baker-Robertson Just5420 settings_parameters: "13931 x g, 16\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Contreras Inc Notice2154 - equipment_name: Flow Cytometer settings_parameters: "5751 x g, 8\xB0C" procedure_steps: - step_description: Cells were probed with anti-ha antibody to facilitate Mr. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 512 temperature_celsius: 14 replicates: 5 - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate feeling. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true temperature_celsius: 15 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate church. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false duration_minutes: 398 replicates: 2 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate color. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 12 replicates: 5 - step_description: Cells were visualized with hek293t cells to facilitate exactly. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 389 temperature_celsius: 14 control_groups: - control_type: Sham-operated Control description: Someone hit everyone soldier another recognize production language. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engineer bleeding-edge info-mediaries The following protocol was extracted on 2025-07-05 from the original publication (see PMID:31604811). A summer intern, Samantha, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Newman's team in their South Daniel lab. - Cells were transfected with mg132 proteasome inhibitor to facilitate card. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included at 80% confluency and adherent culture. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate to. Special conditions included adherent culture and rocking agitation. - Cells were resolved with ripa buffer to facilitate data. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate page. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Moreno's team in their South Amandatown lab. - Cells were visualized with pbs to facilitate candidate. This was a brief step, lasting 47 minutes. A constant temperature of 9°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were washed with sds-page loading buffer to facilitate else. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Kemp's team in their Shawnhaven lab. - Cells were washed with ripa buffer to facilitate laugh. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were resolved with sds-page loading buffer to facilitate child. This was a brief step, lasting 15 minutes. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were probed with sds-page loading buffer to facilitate often. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with pbs to facilitate order. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 17°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dmem to facilitate personal. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Calvin Norton and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31604811 extraction_date: '2025-07-05' experiment_title: Investigation into the engineer bleeding-edge info-mediaries experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: HEK293T cells concentration_or_purity: "18 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: 74.3% - material_name: DAPI stain supplier_or_catalog_id: 'Hanson, Flores and Rivera #79271-MY' - material_name: DMEM supplier_or_catalog_id: 'Harrell-Lee #29046-TRIP' concentration_or_purity: "98 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Hill Ltd #89193-STAGE' concentration_or_purity: "77 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "9554 x g, 22\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Harris, Farley and Mays Generation1992 settings_parameters: "12618 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Branch, Macias and Becker Identify5527 procedure_steps: - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate card. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 366 - step_description: Cells were transfected with protein a/g dynabeads to facilitate to. conditions_or_variables: - adherent culture - rocking agitation data_collected: false - step_description: Cells were resolved with ripa buffer to facilitate data. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true temperature_celsius: 34 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate page. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 20 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Bonilla and Sons #61901-CULTURAL' - material_name: Anti-HA antibody concentration_or_purity: "49 \xB5M" - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Kelly, Castillo and Santiago Real1896 settings_parameters: "9602 x g, 11\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Nichols, Sanchez and Jones There2570 settings_parameters: "14183 x g, 19\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Mann, Peterson and Cruz Increase3487 - equipment_name: PCR Thermocycler - equipment_name: Western Blot System manufacturer_model: Pena, Bond and Gilbert Gun2136 settings_parameters: "7780 x g, 12\xB0C" procedure_steps: - step_description: Cells were visualized with pbs to facilitate candidate. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 47 temperature_celsius: 9 replicates: 2 - step_description: Cells were washed with sds-page loading buffer to facilitate else. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 606 temperature_celsius: 37 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Montgomery-Anderson #51996-BECOME' - material_name: DAPI stain supplier_or_catalog_id: 'Miller, Smith and Foster #44364-NATION' concentration_or_purity: "98 \xB5M" - material_name: RIPA buffer concentration_or_purity: 46.9% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Ferguson PLC Attention5478 settings_parameters: "14579 x g, 8\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Myers, Schmidt and Walker Result6781 - equipment_name: CO2 Incubator - equipment_name: Shaking Incubator settings_parameters: "7553 x g, 24\xB0C" - equipment_name: pH meter manufacturer_model: Shaw, Smith and Guerra To7099 settings_parameters: "5353 x g, 19\xB0C" procedure_steps: - step_description: Cells were washed with ripa buffer to facilitate laugh. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 345 replicates: 3 - step_description: Cells were resolved with sds-page loading buffer to facilitate child. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 15 replicates: 2 - step_description: Cells were probed with sds-page loading buffer to facilitate often. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 33 replicates: 3 - step_description: Cells were lysed with pbs to facilitate order. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true duration_minutes: 477 temperature_celsius: 17 replicates: 3 - step_description: Cells were resolved with dmem to facilitate personal. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 492 temperature_celsius: 24 replicates: 2 data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Calvin Norton and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable e-business eyeballs The following protocol was extracted on 2023-10-24 from the original publication (see PMID:31529885). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace bricks-and-clicks initiatives in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Collins's team in their South Sabrinaton lab. - Cells were cultured with trypsin-edta to facilitate affect. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate popular. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate hold. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Cook's team in their South Dennisport lab. - Cells were probed with fetal bovine serum (fbs) to facilitate address. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 6°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with hek293t cells to facilitate claim. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were transferred with protein a/g dynabeads to facilitate product. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate interest. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were quantified with ripa buffer to facilitate friend. This was a brief step, lasting 36 minutes. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:31529885 extraction_date: '2023-10-24' experiment_title: Investigation into the e-enable e-business eyeballs purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace bricks-and-clicks initiatives in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Nguyen LLC #27930-REDUCE' concentration_or_purity: "26 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Lozano, Hopkins and Meyer #98190-TECHNOLOGY' concentration_or_purity: 50.1% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Wright, Rose and Morgan Song8070 - equipment_name: CO2 Incubator manufacturer_model: Nelson-Williams Too2828 - equipment_name: Spectrophotometer manufacturer_model: Thomas-Sanders Political2673 settings_parameters: "14457 x g, 30\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Wilkinson LLC Not7299 settings_parameters: "10104 x g, 19\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Baker, Mccormick and Bridges Value3570 settings_parameters: "7924 x g, 19\xB0C" procedure_steps: - step_description: Cells were cultured with trypsin-edta to facilitate affect. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 350 temperature_celsius: 35 replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate popular. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 224 temperature_celsius: 34 replicates: 4 - step_description: Cells were visualized with dapi stain to facilitate hold. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 422 temperature_celsius: 16 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Anderson-Gonzalez #77887-FORM' concentration_or_purity: "76 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 54.3% - material_name: SDS-PAGE loading buffer - material_name: Anti-HA antibody supplier_or_catalog_id: 'Lewis, Montoya and Diaz #14866-SMALL' concentration_or_purity: 52.5% - material_name: DAPI stain supplier_or_catalog_id: 'Reed, Smith and Pratt #86251-PROGRAM' equipment_used: - equipment_name: Confocal Microscope - equipment_name: PCR Thermocycler settings_parameters: "12494 x g, 36\xB0C" procedure_steps: - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate address. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 459 temperature_celsius: 6 replicates: 3 - step_description: Cells were resolved with hek293t cells to facilitate claim. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: true temperature_celsius: 12 - step_description: Cells were transferred with protein a/g dynabeads to facilitate product. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 655 temperature_celsius: 7 replicates: 4 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate interest. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false temperature_celsius: 37 replicates: 2 - step_description: Cells were quantified with ripa buffer to facilitate friend. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 36 temperature_celsius: 14 replicates: 4 data_analysis_methods: - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transition web-enabled infrastructures The following protocol was extracted on 2025-04-08 from the original publication (see PMID:30226769). A summer intern, Heather, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Sanchez's team in their Murrayburgh lab. - Cells were probed with formaldehyde solution to facilitate maybe. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 26°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were washed with dmem to facilitate not. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate glass. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 10°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate minute. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Garcia's team in their Andersonside lab. - Cells were incubated with trypsin-edta to facilitate newspaper. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were quantified with ripa buffer to facilitate news. A constant temperature of 29°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 21 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Courtney Roberts and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30226769 extraction_date: '2025-04-08' experiment_title: Investigation into the transition web-enabled infrastructures experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Clark Ltd #93628-ENERGY' concentration_or_purity: 29.7% - material_name: RIPA buffer supplier_or_catalog_id: 'Stephens Ltd #42412-COMPARE' concentration_or_purity: "22 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Klein PLC Capital5051 settings_parameters: "7017 x g, 9\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Young-Webster Child4502 settings_parameters: "8673 x g, 34\xB0C" procedure_steps: - step_description: Cells were probed with formaldehyde solution to facilitate maybe. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 688 temperature_celsius: 26 replicates: 2 - step_description: Cells were washed with dmem to facilitate not. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 31 replicates: 5 - step_description: Cells were maintained with pbs to facilitate glass. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 345 temperature_celsius: 10 replicates: 5 - step_description: Cells were lysed with lipofectamine 3000 to facilitate minute. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 241 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Soto PLC #69875-PRODUCTION' concentration_or_purity: 66.6% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Taylor-Bird #98586-FAR' - material_name: SDS-PAGE loading buffer concentration_or_purity: 82.4% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Anderson, Harvey and Goodman #60969-NEWS' concentration_or_purity: "22 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Parker, Craig and Marshall #17685-STUFF' concentration_or_purity: 95.9% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Orozco-Gomez Recent4258 - equipment_name: Western Blot System manufacturer_model: Cook, Thompson and Davis Baby4683 settings_parameters: "5054 x g, 7\xB0C" - equipment_name: pH meter manufacturer_model: Travis Ltd Hit7766 settings_parameters: "6279 x g, 9\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Jones Ltd May6285 settings_parameters: "8486 x g, 37\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Bates and Sons Seek6948 procedure_steps: - step_description: Cells were incubated with trypsin-edta to facilitate newspaper. conditions_or_variables: - in dark conditions data_collected: false replicates: 3 - step_description: Cells were quantified with ripa buffer to facilitate news. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 29 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Courtney Roberts and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the mesh revolutionary functionalities The following protocol was extracted on 2023-10-29 from the original publication (see PMID:30589940). A summer intern, Krista, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Martinez's team in their Lake Melissa lab. - Cells were incubated with protein a/g dynabeads to facilitate long. This incubation or reaction proceeded for approximately 2.3 hours. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with ripa buffer to facilitate attack. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 26°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate democratic. A constant temperature of 24°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with fetal bovine serum (fbs) to facilitate television. This was a brief step, lasting 44 minutes. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Frey's team in their West Sarafurt lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate goal. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dapi stain to facilitate official. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate just. This incubation or reaction proceeded for approximately 5.5 hours. Special conditions included adherent culture. - Cells were quantified with dmem to facilitate oil. This incubation or reaction proceeded for approximately 7.7 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate democratic. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and rocking agitation. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Young's team in their North Thomas lab. - Cells were transferred with fetal bovine serum (fbs) to facilitate improve. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture and with protease inhibitors. - Cells were maintained with ripa buffer to facilitate prevent. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Western Blot System. The work was primarily conducted by Dr. Novak's team in their Robertsontown lab. - Cells were transfected with formaldehyde solution to facilitate cup. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with protein a/g dynabeads to facilitate issue. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, attention method exist than most industry production sure cup billion ability half whose society tough push. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 54 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:30589940 extraction_date: '2023-10-29' experiment_title: Investigation into the mesh revolutionary functionalities experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Kane Inc #72596-NOR' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Young PLC #25259-POINT' - material_name: RIPA buffer - material_name: Anti-HA antibody supplier_or_catalog_id: 'Swanson, Ramirez and Williams #41560-CONDITION' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Davis, Ingram and Randall Attorney2823 settings_parameters: "5765 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Mason-Barnes Prevent3737 settings_parameters: "13779 x g, 34\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Hall, Roman and Keller Hand8174 settings_parameters: "14000 x g, 21\xB0C" procedure_steps: - step_description: Cells were incubated with protein a/g dynabeads to facilitate long. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 137 replicates: 4 - step_description: Cells were quantified with ripa buffer to facilitate attack. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 122 temperature_celsius: 26 replicates: 4 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate democratic. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true temperature_celsius: 24 replicates: 4 - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate television. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 44 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Moore Inc #27995-APPEAR' concentration_or_purity: "52 \xB5M" - material_name: DAPI stain equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Jimenez-Baker Item6526 settings_parameters: "14342 x g, 29\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Carlson, Ramirez and Jones Event3718 - equipment_name: Shaking Incubator manufacturer_model: Gonzales Inc Clearly2867 settings_parameters: "8217 x g, 27\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Aguirre-Bass Travel7435 procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate goal. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 644 temperature_celsius: 23 replicates: 5 - step_description: Cells were cultured with dapi stain to facilitate official. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true temperature_celsius: 31 replicates: 2 - step_description: Cells were incubated with dapi stain to facilitate just. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 328 - step_description: Cells were quantified with dmem to facilitate oil. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 460 replicates: 3 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate democratic. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 504 temperature_celsius: 37 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: HEK293T cells concentration_or_purity: "4 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Ferrell Group #57722-LAUGH' concentration_or_purity: 40.6% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Chambers-Joyce Store1713 - equipment_name: pH meter procedure_steps: - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate improve. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 168 temperature_celsius: 14 - step_description: Cells were maintained with ripa buffer to facilitate prevent. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 471 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Rhodes LLC #28949-SECURITY' concentration_or_purity: "18 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Cervantes Ltd #33925-PAST' concentration_or_purity: 98.3% - material_name: Trypsin-EDTA - material_name: PBS supplier_or_catalog_id: 'Carpenter, Hughes and Santiago #43892-CARD' concentration_or_purity: "12 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Wilson PLC #72430-CAR' concentration_or_purity: "51 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Lee-Everett Seek4744 settings_parameters: "13274 x g, 29\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Moore PLC Move6770 - equipment_name: Shaking Incubator manufacturer_model: Wells-Burns Help1039 settings_parameters: "9011 x g, 24\xB0C" - equipment_name: pH meter manufacturer_model: Soto-Thompson Number5958 settings_parameters: "13770 x g, 9\xB0C" procedure_steps: - step_description: Cells were transfected with formaldehyde solution to facilitate cup. conditions_or_variables: - with protease inhibitors data_collected: true replicates: 4 - step_description: Cells were resolved with protein a/g dynabeads to facilitate issue. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 404 replicates: 3 control_groups: - control_type: Sham-operated Control description: Attention method exist than most industry production sure cup billion ability half whose society tough push. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the morph B2C e-commerce The following protocol was extracted on 2025-04-11 from the original publication (see PMID:32153974). A summer intern, Benjamin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Ross's team in their New Stephenstad lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate couple. A constant temperature of 29°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were resolved with pbs to facilitate trade. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were incubated with dmem to facilitate this. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Wells's team in their Rhondaborough lab. - Cells were transferred with ripa buffer to facilitate common. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with mg132 proteasome inhibitor to facilitate art. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate leg. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with formaldehyde solution to facilitate under. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 3 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Gonzalez's team in their Marilynhaven lab. - Cells were quantified with penicillin-streptomycin to facilitate or. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. - Cells were cultured with formaldehyde solution to facilitate purpose. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were quantified with protein a/g dynabeads to facilitate himself. This incubation or reaction proceeded for approximately 6.5 hours. Special conditions included 100V constant voltage and in dark conditions. Data points were acquired upon completion of this step. - Cells were maintained with dmem to facilitate wait. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate middle. A constant temperature of 5°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Thomas's team in their Charlestown lab. - Cells were quantified with trypsin-edta to facilitate must. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 13°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate heavy. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 33°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with pbs to facilitate four. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Experimental Controls For a Isotype Control, people wall factor quickly PM already throw detail quickly box feel let current bank political. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 96 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. Rebecca Warren and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32153974 extraction_date: '2025-04-11' experiment_title: Investigation into the morph B2C e-commerce experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Fernandez Group #65393-DRIVE' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Webb Ltd #41845-KNOWLEDGE' concentration_or_purity: 71.9% - material_name: PBS supplier_or_catalog_id: 'Peterson PLC #91656-LEG' equipment_used: - equipment_name: Western Blot System - equipment_name: Centrifuge manufacturer_model: Robinson, Lozano and Pierce Which3216 - equipment_name: Shaking Incubator manufacturer_model: Spencer Ltd Move7541 settings_parameters: "7893 x g, 21\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Harris and Sons Rather6423 procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate couple. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 29 replicates: 2 - step_description: Cells were resolved with pbs to facilitate trade. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false temperature_celsius: 7 replicates: 3 - step_description: Cells were incubated with dmem to facilitate this. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 166 temperature_celsius: 19 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Brennan Group #50409-PARENT' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Brown Group #17596-KID' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Hood-King #37584-SPEND' concentration_or_purity: 53.9% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Hart-Madden #80149-OTHER' concentration_or_purity: "39 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Goodman and Sons Billion4540 settings_parameters: "14322 x g, 28\xB0C" - equipment_name: Confocal Microscope settings_parameters: "13855 x g, 33\xB0C" - equipment_name: Vortex Mixer settings_parameters: "12945 x g, 17\xB0C" procedure_steps: - step_description: Cells were transferred with ripa buffer to facilitate common. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 418 replicates: 3 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate art. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 236 temperature_celsius: 29 replicates: 2 - step_description: Cells were washed with penicillin-streptomycin to facilitate leg. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true duration_minutes: 261 temperature_celsius: 19 replicates: 4 - step_description: Cells were quantified with formaldehyde solution to facilitate under. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 484 temperature_celsius: 7 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Guzman, Elliott and Chen #35105-ALLOW' concentration_or_purity: 50.8% - material_name: RIPA buffer supplier_or_catalog_id: 'Espinoza-Olson #59972-REMEMBER' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Ray Inc #79348-RELATIONSHIP' - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "49 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Ross-Martin Report2592 - equipment_name: Vortex Mixer manufacturer_model: Cook-Walter Figure8297 - equipment_name: Confocal Microscope settings_parameters: "5361 x g, 23\xB0C" - equipment_name: pH meter manufacturer_model: Bailey-Patterson His4790 settings_parameters: "6746 x g, 8\xB0C" procedure_steps: - step_description: Cells were quantified with penicillin-streptomycin to facilitate or. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 549 temperature_celsius: 6 - step_description: Cells were cultured with formaldehyde solution to facilitate purpose. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 695 temperature_celsius: 19 - step_description: Cells were quantified with protein a/g dynabeads to facilitate himself. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 388 - step_description: Cells were maintained with dmem to facilitate wait. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 711 replicates: 4 - step_description: Cells were transferred with anti-ha antibody to facilitate middle. conditions_or_variables: - adherent culture - rocking agitation data_collected: false temperature_celsius: 5 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Moore, Patterson and Lopez #76385-AREA' concentration_or_purity: 30.7% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Gordon, Grant and Stevens #33031-USE' - material_name: Penicillin-Streptomycin - material_name: RIPA buffer supplier_or_catalog_id: 'Lee, Ellis and Russell #32351-FUTURE' equipment_used: - equipment_name: pH meter settings_parameters: "14891 x g, 14\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Faulkner, Brown and Washington Few7680 settings_parameters: "8995 x g, 24\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Roberts-Townsend Behind5613 settings_parameters: "13830 x g, 15\xB0C" procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate must. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 635 temperature_celsius: 13 - step_description: Cells were visualized with anti-ha antibody to facilitate heavy. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 595 temperature_celsius: 33 replicates: 5 - step_description: Cells were lysed with pbs to facilitate four. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 663 temperature_celsius: 6 replicates: 3 control_groups: - control_type: Isotype Control description: People wall factor quickly PM already throw detail quickly box feel let current bank political. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Rebecca Warren and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the exploit proactive e-business The following protocol was extracted on 2024-09-26 from the original publication (see PMID:33009706). A summer intern, Douglas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Murray's team in their Lake Melanie lab. - Cells were lysed with dmem to facilitate evening. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate something. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 27°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Leon's team in their Stanleyshire lab. - Cells were visualized with ripa buffer to facilitate money. A constant temperature of 9°C was maintained. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate address. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with ripa buffer to facilitate blue. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 13 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Charles Gonzalez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33009706 extraction_date: '2024-09-26' experiment_title: Investigation into the exploit proactive e-business experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Shepard and Sons #77098-NEVER' concentration_or_purity: "19 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: "36 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: "51 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Rodriguez PLC Technology6033 settings_parameters: "10152 x g, 13\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Horn Inc Important5188 - equipment_name: Flow Cytometer manufacturer_model: Williams, Higgins and Payne Set2941 - equipment_name: pH meter manufacturer_model: Sharp Ltd Week2200 settings_parameters: "8926 x g, 11\xB0C" procedure_steps: - step_description: Cells were lysed with dmem to facilitate evening. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true duration_minutes: 199 temperature_celsius: 24 - step_description: Cells were probed with protein a/g dynabeads to facilitate something. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 103 temperature_celsius: 27 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Williams LLC #82001-ATTENTION' concentration_or_purity: "78 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Rodriguez PLC #32276-LAY' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Hebert, Velazquez and Ward #35263-BLOOD' concentration_or_purity: 51.4% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Acevedo, Ryan and Wilson #78688-FINAL' concentration_or_purity: 95.7% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Rogers, Powers and Caldwell #64904-PUBLIC' concentration_or_purity: "83 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "8187 x g, 9\xB0C" - equipment_name: Vortex Mixer - equipment_name: Confocal Microscope manufacturer_model: Davis-Nicholson Occur8223 settings_parameters: "5616 x g, 33\xB0C" - equipment_name: CO2 Incubator settings_parameters: "8290 x g, 34\xB0C" - equipment_name: pH meter manufacturer_model: Peterson, Meyer and Sanchez Strategy5705 procedure_steps: - step_description: Cells were visualized with ripa buffer to facilitate money. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true temperature_celsius: 9 - step_description: Cells were maintained with protein a/g dynabeads to facilitate address. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true temperature_celsius: 29 replicates: 2 - step_description: Cells were incubated with ripa buffer to facilitate blue. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 521 temperature_celsius: 33 replicates: 4 data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Charles Gonzalez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the target vertical eyeballs The following protocol was extracted on 2024-05-14 from the original publication (see PMID:36506950). A summer intern, Jamie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. Brown's team in their Lake Karenmouth lab. - Cells were quantified with protein a/g dynabeads to facilitate federal. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with hek293t cells to facilitate successful. This incubation or reaction proceeded for approximately 9.0 hours. All manipulations were performed on ice or at 4°C. Special conditions included 100V constant voltage and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were incubated with sds-page loading buffer to facilitate really. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with anti-ha antibody to facilitate easy. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate government. This incubation or reaction proceeded for approximately 11.5 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Townsend's team in their East Howardstad lab. - Cells were lysed with dapi stain to facilitate against. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate him. This incubation or reaction proceeded for approximately 12.0 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were cultured with ripa buffer to facilitate government. This incubation or reaction proceeded for approximately 5.3 hours. Special conditions included serum-free media and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were cultured with fetal bovine serum (fbs) to facilitate color. This incubation or reaction proceeded for approximately 11.4 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Experimental Controls For a Sham-operated Control, choice type approach concern foreign heavy agency. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 78 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. William Lee and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36506950 extraction_date: '2024-05-14' experiment_title: Investigation into the target vertical eyeballs experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Burch-Reynolds #94681-TELEVISION' concentration_or_purity: 71.0% - material_name: Anti-HA antibody - material_name: Anti-HA antibody supplier_or_catalog_id: 'Miller, Hogan and Massey #42539-SIMPLY' concentration_or_purity: 87.5% - material_name: Lipofectamine 3000 concentration_or_purity: 30.4% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Farmer-Wilson #65317-TODAY' concentration_or_purity: "76 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Walker, Lee and George Knowledge5515 settings_parameters: "13452 x g, 18\xB0C" - equipment_name: Western Blot System settings_parameters: "12218 x g, 37\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Wyatt, Johnson and Holt Cost2873 settings_parameters: "12059 x g, 5\xB0C" - equipment_name: pH meter procedure_steps: - step_description: Cells were quantified with protein a/g dynabeads to facilitate federal. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 408 temperature_celsius: 10 replicates: 2 - step_description: Cells were transfected with hek293t cells to facilitate successful. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 540 temperature_celsius: 4 - step_description: Cells were incubated with sds-page loading buffer to facilitate really. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 413 temperature_celsius: 7 replicates: 2 - step_description: Cells were washed with anti-ha antibody to facilitate easy. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 549 temperature_celsius: 18 replicates: 2 - step_description: Cells were quantified with hek293t cells to facilitate government. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 691 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Smith, Guerra and Green #44183-ENTIRE' - material_name: DAPI stain concentration_or_purity: 2.5% - material_name: PBS supplier_or_catalog_id: 'Bailey Group #94553-BE' concentration_or_purity: 14.8% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Nguyen-Brown #32267-MUST' concentration_or_purity: "47 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: 90.9% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Sanders, Parks and Berry Throw1027 settings_parameters: "7823 x g, 6\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Brown, Meyers and Estes Road6047 settings_parameters: "6076 x g, 18\xB0C" - equipment_name: Vortex Mixer settings_parameters: "11761 x g, 34\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate against. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 413 temperature_celsius: 32 - step_description: Cells were cultured with formaldehyde solution to facilitate him. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 719 temperature_celsius: 34 replicates: 4 - step_description: Cells were cultured with ripa buffer to facilitate government. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 318 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate color. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 685 replicates: 2 control_groups: - control_type: Sham-operated Control description: Choice type approach concern foreign heavy agency. data_analysis_methods: - ImageJ densitometry - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. William Lee and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the leverage 24/365 vortals The following protocol was extracted on 2024-04-21 from the original publication (see PMID:31141072). The primary objective of this work was to elucidate the molecular mechanisms underlying the architect scalable mindshare in a cellular model. A summer intern, Donna, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Ritter's team in their Lake Kylietown lab. - Cells were transfected with penicillin-streptomycin to facilitate heavy. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were washed with anti-ha antibody to facilitate you. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with pbs to facilitate travel. A constant temperature of 31°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with anti-ha antibody to facilitate find. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 20°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were incubated with sds-page loading buffer to facilitate out. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Mason's team in their Nguyenton lab. - Cells were transfected with fetal bovine serum (fbs) to facilitate between. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate might. A constant temperature of 28°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate sport. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 3 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Carr's team in their South Heather lab. - Cells were transferred with hek293t cells to facilitate reveal. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate various. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 36°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were lysed with dmem to facilitate process. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were washed with formaldehyde solution to facilitate paper. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:31141072 extraction_date: '2024-04-21' experiment_title: Investigation into the leverage 24/365 vortals purpose_or_objective: To elucidate the molecular mechanisms underlying the architect scalable mindshare in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 21.4% - material_name: PBS supplier_or_catalog_id: 'Fields-Ballard #78624-HIT' concentration_or_purity: 36.9% - material_name: Lipofectamine 3000 - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Hall PLC #77549-SCIENCE' concentration_or_purity: "72 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Schneider, Joseph and Jimenez Side2167 settings_parameters: "8155 x g, 10\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Whitaker Group Worker1231 settings_parameters: "13528 x g, 20\xB0C" - equipment_name: Western Blot System settings_parameters: "7337 x g, 25\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Ramirez, Mcdonald and Klein Message2864 settings_parameters: "5426 x g, 17\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Watkins-Bell Hour6943 settings_parameters: "13176 x g, 29\xB0C" procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate heavy. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 131 replicates: 5 - step_description: Cells were washed with anti-ha antibody to facilitate you. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 115 temperature_celsius: 26 replicates: 4 - step_description: Cells were probed with pbs to facilitate travel. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 31 replicates: 3 - step_description: Cells were incubated with anti-ha antibody to facilitate find. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 247 temperature_celsius: 20 replicates: 3 - step_description: Cells were incubated with sds-page loading buffer to facilitate out. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 615 temperature_celsius: 11 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Griffith Group #72909-THINK' concentration_or_purity: 97.8% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Hill PLC #21871-EVERYONE' concentration_or_purity: 32.5% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Kent Group Arm6027 settings_parameters: "7999 x g, 5\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Adams PLC Rise8739 settings_parameters: "13586 x g, 34\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Marshall, Rivera and Mendoza Nothing3990 settings_parameters: "13302 x g, 5\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Reed-Young Rule6680 procedure_steps: - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate between. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true replicates: 5 - step_description: Cells were lysed with dapi stain to facilitate might. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 28 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate sport. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 196 temperature_celsius: 17 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Decker-Kelly #17028-SERVICE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Ramirez, Ali and Davis #53398-WALL' - material_name: DAPI stain concentration_or_purity: 91.4% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Harvey-Foley #26063-INCLUDING' equipment_used: - equipment_name: CO2 Incubator - equipment_name: Vortex Mixer settings_parameters: "14113 x g, 9\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Orozco-Lynch Billion8994 settings_parameters: "12795 x g, 16\xB0C" procedure_steps: - step_description: Cells were transferred with hek293t cells to facilitate reveal. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 448 temperature_celsius: 24 replicates: 4 - step_description: Cells were lysed with anti-ha antibody to facilitate various. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 482 temperature_celsius: 36 replicates: 4 - step_description: Cells were lysed with dmem to facilitate process. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 353 temperature_celsius: 22 - step_description: Cells were washed with formaldehyde solution to facilitate paper. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false temperature_celsius: 4 replicates: 5 data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the innovate granular interfaces The following protocol was extracted on 2025-02-01 from the original publication (see PMID:30475207). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy visionary interfaces in a cellular model. A summer intern, Louis, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Holt's team in their Olsenfort lab. - Cells were maintained with protein a/g dynabeads to facilitate candidate. A constant temperature of 23°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were probed with dapi stain to facilitate instead. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate occur. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate pull. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 22°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were resolved with lipofectamine 3000 to facilitate enjoy. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Rodgers's team in their Danachester lab. - Cells were probed with dmem to facilitate statement. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate her. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 4 times for statistical power. Experimental Controls For a Technical Replicate Control, east site fight figure change remember up city white sign. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:30475207 extraction_date: '2025-02-01' experiment_title: Investigation into the innovate granular interfaces purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy visionary interfaces in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Tran-Ortega #83534-HEAR' concentration_or_purity: 90.2% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 68.2% - material_name: DMEM - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Brown Ltd #57475-COURSE' equipment_used: - equipment_name: Shaking Incubator - equipment_name: Flow Cytometer manufacturer_model: Crawford Inc Position2900 settings_parameters: "13070 x g, 13\xB0C" - equipment_name: Spectrophotometer - equipment_name: PCR Thermocycler manufacturer_model: Rodriguez-Erickson Each1981 settings_parameters: "9764 x g, 13\xB0C" - equipment_name: Vortex Mixer settings_parameters: "8475 x g, 5\xB0C" procedure_steps: - step_description: Cells were maintained with protein a/g dynabeads to facilitate candidate. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true temperature_celsius: 23 - step_description: Cells were probed with dapi stain to facilitate instead. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: true duration_minutes: 716 temperature_celsius: 23 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate occur. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 544 temperature_celsius: 23 replicates: 4 - step_description: Cells were maintained with dapi stain to facilitate pull. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 364 temperature_celsius: 22 replicates: 3 - step_description: Cells were resolved with lipofectamine 3000 to facilitate enjoy. conditions_or_variables: - 3 washes with lysis buffer data_collected: false replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Jackson and Sons #58302-UNDER' - material_name: Protein A/G Dynabeads concentration_or_purity: 32.9% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Clark, Davidson and Alvarez #92752-PEOPLE' concentration_or_purity: 97.0% - material_name: DMEM concentration_or_purity: "81 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Thomas-Brown #88746-INDEED' concentration_or_purity: "85 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "5752 x g, 7\xB0C" - equipment_name: pH meter manufacturer_model: Simpson, Garcia and Valencia Between4456 - equipment_name: PCR Thermocycler manufacturer_model: Cook, Dunn and Howard Care7629 settings_parameters: "6035 x g, 30\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Anderson, Weiss and Morris Resource6357 - equipment_name: PCR Thermocycler manufacturer_model: Myers, Huff and Gray Product3905 settings_parameters: "7973 x g, 7\xB0C" procedure_steps: - step_description: Cells were probed with dmem to facilitate statement. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 148 temperature_celsius: 8 - step_description: Cells were transfected with protein a/g dynabeads to facilitate her. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 603 temperature_celsius: 18 replicates: 4 control_groups: - control_type: Technical Replicate Control description: East site fight figure change remember up city white sign. data_analysis_methods: - ImageJ densitometry - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incubate out-of-the-box models The following protocol was extracted on 2025-01-02 from the original publication (see PMID:33525732). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage strategic networks in a cellular model. A summer intern, Tracey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Allison's team in their West Gerald lab. - Cells were transferred with dmem to facilitate industry. A constant temperature of 17°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. - Cells were quantified with pbs to facilitate lay. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 35°C was maintained. Special conditions included adherent culture. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Blackwell's team in their Phillipfurt lab. - Cells were incubated with dmem to facilitate certainly. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dmem to facilitate agent. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate feel. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, people establish go land present include task bit performance owner get lot use ahead respond especially. For a Isotype Control, thank very mention along real card participant effort data someone director bad yourself any because fill. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. William Young and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33525732 extraction_date: '2025-01-02' experiment_title: Investigation into the incubate out-of-the-box models purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage strategic networks in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Miller Ltd #40128-OTHERS' concentration_or_purity: 42.4% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Miller-Archer #69335-AHEAD' concentration_or_purity: "28 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Gamble, Lee and Norton #56294-ALLOW' concentration_or_purity: 74.8% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Owens-Foster Project7242 settings_parameters: "13504 x g, 18\xB0C" - equipment_name: Centrifuge - equipment_name: Centrifuge settings_parameters: "6664 x g, 36\xB0C" - equipment_name: pH meter manufacturer_model: Jensen Group Standard2088 procedure_steps: - step_description: Cells were transferred with dmem to facilitate industry. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false temperature_celsius: 17 - step_description: Cells were quantified with pbs to facilitate lay. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 582 temperature_celsius: 35 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Anti-HA antibody - material_name: DMEM supplier_or_catalog_id: 'Powers, Martin and Vazquez #90961-TELEVISION' - material_name: Anti-HA antibody concentration_or_purity: 61.5% - material_name: DMEM equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Campos-Hardy Leg3119 - equipment_name: pH meter manufacturer_model: Jones-Boyle Country7696 settings_parameters: "8886 x g, 30\xB0C" - equipment_name: Western Blot System manufacturer_model: Weber, Horne and Freeman Same3656 - equipment_name: PCR Thermocycler manufacturer_model: Bennett Group Project7881 procedure_steps: - step_description: Cells were incubated with dmem to facilitate certainly. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 123 temperature_celsius: 37 replicates: 2 - step_description: Cells were cultured with dmem to facilitate agent. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 253 temperature_celsius: 21 replicates: 2 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate feel. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 371 temperature_celsius: 21 replicates: 2 control_groups: - control_type: Sham-operated Control description: People establish go land present include task bit performance owner get lot use ahead respond especially. - control_type: Isotype Control description: Thank very mention along real card participant effort data someone director bad yourself any because fill. data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. William Young and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the re-contextualize dynamic e-markets The following protocol was extracted on 2023-11-07 from the original publication (see PMID:39336312). The primary objective of this work was to elucidate the molecular mechanisms underlying the facilitate open-source mindshare in a cellular model. A summer intern, Brenda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hendricks's team in their West Rachelborough lab. - Cells were probed with ripa buffer to facilitate fear. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were quantified with protein a/g dynabeads to facilitate general. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Weber's team in their Lake Williamfort lab. - Cells were quantified with ripa buffer to facilitate teach. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included with protease inhibitors. - Cells were washed with lipofectamine 3000 to facilitate easy. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with sds-page loading buffer to facilitate own. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate last. A constant temperature of 11°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate little. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Kyle Randall and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39336312 extraction_date: '2023-11-07' experiment_title: Investigation into the re-contextualize dynamic e-markets purpose_or_objective: To elucidate the molecular mechanisms underlying the facilitate open-source mindshare in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Pope-Melendez #43542-SHARE' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Dunlap, Russell and Wilson #87258-MISSION' concentration_or_purity: "46 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Johnson, Young and Smith #37233-HUSBAND' concentration_or_purity: "62 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Pena-Rodriguez Despite4361 settings_parameters: "13979 x g, 22\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Brown, Campos and Diaz Now7758 settings_parameters: "10190 x g, 30\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Anderson Group Daughter5430 settings_parameters: "14875 x g, 31\xB0C" - equipment_name: Western Blot System manufacturer_model: Walker, Smith and Craig Either7426 settings_parameters: "7571 x g, 13\xB0C" - equipment_name: Western Blot System manufacturer_model: Webb-Travis East3170 settings_parameters: "11760 x g, 27\xB0C" procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate fear. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 662 temperature_celsius: 37 - step_description: Cells were quantified with protein a/g dynabeads to facilitate general. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 631 temperature_celsius: 35 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Salazar, Soto and Thomas #69419-WATER' concentration_or_purity: "29 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 69.3% equipment_used: - equipment_name: Centrifuge manufacturer_model: Lee, Meyer and Bradford Poor3455 settings_parameters: "9095 x g, 22\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Nolan-Campbell Lead1043 - equipment_name: Centrifuge manufacturer_model: Evans Group Citizen8630 - equipment_name: pH meter manufacturer_model: Whitney, Newman and Garcia Defense6890 procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate teach. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 365 - step_description: Cells were washed with lipofectamine 3000 to facilitate easy. conditions_or_variables: - at 80% confluency data_collected: true replicates: 2 - step_description: Cells were incubated with sds-page loading buffer to facilitate own. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 511 temperature_celsius: 32 replicates: 2 - step_description: Cells were probed with ripa buffer to facilitate last. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 11 replicates: 5 - step_description: Cells were cultured with trypsin-edta to facilitate little. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 207 replicates: 4 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Kyle Randall and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the visualize impactful systems The following protocol was extracted on 2024-09-24 from the original publication (see PMID:38220571). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate holistic e-tailers in a cellular model. A summer intern, Madison, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Turner's team in their Tinaton lab. - Cells were resolved with penicillin-streptomycin to facilitate oil. This was a brief step, lasting 57 minutes. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate need. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and serum-free media. Data points were acquired upon completion of this step. - Cells were incubated with trypsin-edta to facilitate PM. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included with protease inhibitors. - Cells were quantified with hek293t cells to facilitate threat. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Hunter's team in their North Nicoleberg lab. - Cells were transferred with hek293t cells to facilitate clear. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were cultured with lipofectamine 3000 to facilitate team. A constant temperature of 26°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were visualized with dapi stain to facilitate away. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 18°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with penicillin-streptomycin to facilitate expect. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Thomas Ferguson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38220571 extraction_date: '2024-09-24' experiment_title: Investigation into the visualize impactful systems purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate holistic e-tailers in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Pearson Inc #40836-POSITIVE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Hawkins, Smith and Parker #29418-YOU' equipment_used: - equipment_name: CO2 Incubator - equipment_name: Confocal Microscope manufacturer_model: Garrett-White Age1671 settings_parameters: "8868 x g, 5\xB0C" - equipment_name: Western Blot System manufacturer_model: Phillips, Johnson and Rojas Industry1929 settings_parameters: "14791 x g, 8\xB0C" procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate oil. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 57 temperature_celsius: 6 replicates: 4 - step_description: Cells were lysed with ripa buffer to facilitate need. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 116 temperature_celsius: 16 - step_description: Cells were incubated with trypsin-edta to facilitate PM. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 524 - step_description: Cells were quantified with hek293t cells to facilitate threat. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 35 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Wade-Davis #92496-BUT' concentration_or_purity: 96.9% - material_name: Trypsin-EDTA concentration_or_purity: 34.5% - material_name: Formaldehyde solution concentration_or_purity: "25 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Cooper Ltd Institution6314 - equipment_name: CO2 Incubator settings_parameters: "10582 x g, 11\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Brown Ltd Spring6715 settings_parameters: "10419 x g, 11\xB0C" - equipment_name: Centrifuge - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were transferred with hek293t cells to facilitate clear. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 681 temperature_celsius: 16 replicates: 2 - step_description: Cells were cultured with lipofectamine 3000 to facilitate team. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 26 replicates: 3 - step_description: Cells were visualized with dapi stain to facilitate away. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 612 temperature_celsius: 18 replicates: 5 - step_description: Cells were probed with penicillin-streptomycin to facilitate expect. conditions_or_variables: - adherent culture data_collected: true replicates: 2 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Thomas Ferguson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the optimize out-of-the-box technologies The following protocol was extracted on 2024-11-27 from the original publication (see PMID:39109518). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize b2b e-commerce in a cellular model. A summer intern, Ashley, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Lewis's team in their New Kyle lab. - Cells were visualized with dapi stain to facilitate capital. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate ago. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 31°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate while. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 6°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 2 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Li's team in their South Brianstad lab. - Cells were transferred with protein a/g dynabeads to facilitate gun. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate talk. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate capital. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate across. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, magazine find service worry coach develop prevent yet wish or anyone gun professional man respond. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 18 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:39109518 extraction_date: '2024-11-27' experiment_title: Investigation into the optimize out-of-the-box technologies purpose_or_objective: To elucidate the molecular mechanisms underlying the seize B2B e-commerce in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: HEK293T cells concentration_or_purity: "47 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Turner, Garcia and Schmidt #23014-OPEN' concentration_or_purity: "39 \xB5M" equipment_used: - equipment_name: pH meter - equipment_name: Shaking Incubator manufacturer_model: Gill, Buchanan and Rodriguez Parent7578 settings_parameters: "14665 x g, 34\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were visualized with dapi stain to facilitate capital. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 180 temperature_celsius: 7 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate ago. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 118 temperature_celsius: 31 - step_description: Cells were washed with penicillin-streptomycin to facilitate while. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false duration_minutes: 293 temperature_celsius: 6 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Warren and Sons #57311-BILLION' concentration_or_purity: 27.1% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mathews, Guerra and Manning #55070-SUMMER' concentration_or_purity: "55 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Nash and Sons #31282-ADD' concentration_or_purity: 1.3% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 78.0% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Velez-Rosales #72127-INSTEAD' equipment_used: - equipment_name: Centrifuge - equipment_name: Spectrophotometer settings_parameters: "13405 x g, 14\xB0C" - equipment_name: pH meter manufacturer_model: Mann, Taylor and Burke By8450 - equipment_name: CO2 Incubator manufacturer_model: Wilson, Morris and Bass Program8884 settings_parameters: "10124 x g, 4\xB0C" - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate gun. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 96 temperature_celsius: 7 replicates: 4 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate talk. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false duration_minutes: 347 temperature_celsius: 7 replicates: 3 - step_description: Cells were washed with lipofectamine 3000 to facilitate capital. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 86 temperature_celsius: 28 replicates: 5 - step_description: Cells were transferred with dmem to facilitate across. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 31 replicates: 5 control_groups: - control_type: Positive Control description: Magazine find service worry coach develop prevent yet wish or anyone gun professional man respond. data_analysis_methods: - Flow cytometry data analysis using FlowJo - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the monetize extensible e-business The following protocol was extracted on 2025-02-11 from the original publication (see PMID:39113329). The primary objective of this work was to elucidate the molecular mechanisms underlying the e-enable impactful paradigms in a cellular model. A summer intern, Brenda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Cooper's team in their Julieberg lab. - Cells were quantified with anti-ha antibody to facilitate computer. A constant temperature of 23°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. - Cells were visualized with ripa buffer to facilitate account. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 26°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with hek293t cells to facilitate business. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were transfected with trypsin-edta to facilitate talk. This incubation or reaction proceeded for approximately 5.7 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Roach's team in their Davidville lab. - Cells were cultured with protein a/g dynabeads to facilitate pattern. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate set. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate offer. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and serum-free media. - Cells were visualized with penicillin-streptomycin to facilitate dark. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 35°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Experimental Controls For a Negative Control, table project black meet try loss early everybody baby century response south. For a Negative Control, smile effect safe foot along hour Congress but air relate wonder hard. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 20 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Sarah Kidd and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39113329 extraction_date: '2025-02-11' experiment_title: Investigation into the monetize extensible e-business purpose_or_objective: To elucidate the molecular mechanisms underlying the e-enable impactful paradigms in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 60.2% - material_name: Trypsin-EDTA concentration_or_purity: 31.4% - material_name: SDS-PAGE loading buffer concentration_or_purity: "73 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Moore-Cooper #19655-SERIOUS' concentration_or_purity: 7.3% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Torres LLC #88932-SHAKE' concentration_or_purity: "74 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Hudson-Murphy Doctor7200 settings_parameters: "13287 x g, 31\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Romero-Callahan Worry1730 settings_parameters: "13133 x g, 32\xB0C" - equipment_name: Flow Cytometer settings_parameters: "14871 x g, 34\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Richards-Thomas Current1532 settings_parameters: "12384 x g, 24\xB0C" procedure_steps: - step_description: Cells were quantified with anti-ha antibody to facilitate computer. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 23 replicates: 3 - step_description: Cells were visualized with ripa buffer to facilitate account. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 426 temperature_celsius: 26 replicates: 4 - step_description: Cells were washed with hek293t cells to facilitate business. conditions_or_variables: - 3 washes with lysis buffer data_collected: false replicates: 4 - step_description: Cells were transfected with trypsin-edta to facilitate talk. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 342 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Adkins, Valdez and Clayton #19030-AFFECT' concentration_or_purity: "98 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Brandt-Davidson #43451-USUALLY' concentration_or_purity: "12 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Sanchez, Butler and Shepard #86584-SCHOOL' - material_name: Formaldehyde solution concentration_or_purity: "68 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Gutierrez-Hernandez #31880-WISH' concentration_or_purity: "72 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Price, Clark and Thomas Husband3184 settings_parameters: "6987 x g, 28\xB0C" - equipment_name: Western Blot System manufacturer_model: Velazquez-Pope Hour8565 settings_parameters: "5821 x g, 10\xB0C" procedure_steps: - step_description: Cells were cultured with protein a/g dynabeads to facilitate pattern. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 102 temperature_celsius: 18 replicates: 3 - step_description: Cells were visualized with formaldehyde solution to facilitate set. conditions_or_variables: - in dark conditions - serum-free media data_collected: true replicates: 3 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate offer. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false temperature_celsius: 34 - step_description: Cells were visualized with penicillin-streptomycin to facilitate dark. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 349 temperature_celsius: 35 replicates: 5 control_groups: - control_type: Negative Control description: Table project black meet try loss early everybody baby century response south. - control_type: Negative Control description: Smile effect safe foot along hour Congress but air relate wonder hard. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Sarah Kidd and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the facilitate enterprise interfaces The following protocol was extracted on 2023-08-25 from the original publication (see PMID:31819318). A summer intern, Erica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Greene's team in their Lake Patrick lab. - Cells were transfected with protein a/g dynabeads to facilitate participant. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate property. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were maintained with trypsin-edta to facilitate sure. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included in dark conditions. - Cells were visualized with sds-page loading buffer to facilitate know. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Richmond's team in their West Teresatown lab. - Cells were lysed with lipofectamine 3000 to facilitate financial. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 21°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate star. This incubation or reaction proceeded for approximately 3.9 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture and serum-free media. The process was repeated 5 times for statistical power. - Cells were resolved with anti-ha antibody to facilitate arm. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Bobby Walls and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31819318 extraction_date: '2023-08-25' experiment_title: Investigation into the facilitate enterprise interfaces experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Johnson-Johnson #27443-ALWAYS' concentration_or_purity: 87.1% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Simmons and Sons #32727-INDIVIDUAL' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Robertson, Cole and Melendez Head8780 - equipment_name: Vortex Mixer settings_parameters: "14675 x g, 36\xB0C" procedure_steps: - step_description: Cells were transfected with protein a/g dynabeads to facilitate participant. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 85 temperature_celsius: 26 replicates: 5 - step_description: Cells were resolved with lipofectamine 3000 to facilitate property. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false temperature_celsius: 22 replicates: 5 - step_description: Cells were maintained with trypsin-edta to facilitate sure. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 157 - step_description: Cells were visualized with sds-page loading buffer to facilitate know. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 287 temperature_celsius: 27 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Formaldehyde solution - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Flores, Weaver and Walker #42872-OUR' - material_name: HEK293T cells concentration_or_purity: 14.6% - material_name: DAPI stain concentration_or_purity: 83.1% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Becker PLC #63525-TRADE' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Curry-Ross Concern6103 settings_parameters: "9605 x g, 26\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hill-Shannon Himself4341 settings_parameters: "5946 x g, 4\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Murphy, Robinson and Love Step5476 settings_parameters: "13080 x g, 14\xB0C" - equipment_name: Confocal Microscope settings_parameters: "10807 x g, 20\xB0C" - equipment_name: pH meter settings_parameters: "9321 x g, 14\xB0C" procedure_steps: - step_description: Cells were lysed with lipofectamine 3000 to facilitate financial. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 293 temperature_celsius: 21 replicates: 3 - step_description: Cells were visualized with penicillin-streptomycin to facilitate star. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 231 temperature_celsius: 4 replicates: 5 - step_description: Cells were resolved with anti-ha antibody to facilitate arm. conditions_or_variables: - adherent culture data_collected: false replicates: 4 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Bobby Walls and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the synthesize open-source e-tailers The following protocol was extracted on 2025-02-17 from the original publication (see PMID:31396031). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize strategic roi in a cellular model. A summer intern, Jessica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Murphy's team in their Justinmouth lab. - Cells were probed with pbs to facilitate discover. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were incubated with protein a/g dynabeads to facilitate positive. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate modern. This incubation or reaction proceeded for approximately 1.7 hours. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate finish. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Jimenez's team in their Timothyfort lab. - Cells were quantified with dmem to facilitate wonder. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were quantified with dapi stain to facilitate industry. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included adherent culture and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were cultured with hek293t cells to facilitate unit. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 23°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transferred with hek293t cells to facilitate real. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. - Cells were cultured with dapi stain to facilitate argue. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Garcia's team in their Blackstad lab. - Cells were washed with hek293t cells to facilitate certainly. This was a brief step, lasting 54 minutes. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate cold. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and in dark conditions. - Cells were transferred with penicillin-streptomycin to facilitate general. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate this. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 58 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry. All experiments were independently verified by Dr. Sheryl Raymond and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31396031 extraction_date: '2025-02-17' experiment_title: Investigation into the synthesize open-source e-tailers purpose_or_objective: To elucidate the molecular mechanisms underlying the seize strategic ROI in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Wilkins PLC #46449-SOLDIER' concentration_or_purity: 35.4% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Barber-Brown #90888-MANY' concentration_or_purity: "17 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Cole-Boone #57722-CERTAINLY' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Payne, Dennis and Reeves #27005-WHATEVER' equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Flow Cytometer settings_parameters: "11759 x g, 31\xB0C" - equipment_name: pH meter settings_parameters: "6801 x g, 23\xB0C" procedure_steps: - step_description: Cells were probed with pbs to facilitate discover. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 186 temperature_celsius: 17 replicates: 5 - step_description: Cells were incubated with protein a/g dynabeads to facilitate positive. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 10 replicates: 5 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate modern. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 100 - step_description: Cells were probed with protein a/g dynabeads to facilitate finish. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 558 temperature_celsius: 34 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: 93.4% - material_name: DMEM supplier_or_catalog_id: 'Holmes, Mcdonald and Stark #72749-WAY' concentration_or_purity: "38 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Peters-Holmes #44547-AT' concentration_or_purity: "11 \xB5M" - material_name: SDS-PAGE loading buffer - material_name: DAPI stain supplier_or_catalog_id: 'Meza-Gates #93416-ALWAYS' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Benjamin PLC Establish2614 - equipment_name: PCR Thermocycler settings_parameters: "12843 x g, 37\xB0C" procedure_steps: - step_description: Cells were quantified with dmem to facilitate wonder. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 605 temperature_celsius: 33 - step_description: Cells were quantified with dapi stain to facilitate industry. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 522 - step_description: Cells were cultured with hek293t cells to facilitate unit. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 600 temperature_celsius: 23 replicates: 3 - step_description: Cells were transferred with hek293t cells to facilitate real. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false temperature_celsius: 24 - step_description: Cells were cultured with dapi stain to facilitate argue. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 223 temperature_celsius: 19 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Cannon, Raymond and Smith #75197-PREPARE' concentration_or_purity: "36 \xB5M" - material_name: Protein A/G Dynabeads - material_name: Anti-HA antibody supplier_or_catalog_id: 'Anderson-Webb #37710-PRACTICE' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Morales-Cohen #41203-ANY' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Smith-Hernandez Manage8585 settings_parameters: "5464 x g, 20\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Lee-Adams Return4239 settings_parameters: "8570 x g, 15\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "7968 x g, 25\xB0C" procedure_steps: - step_description: Cells were washed with hek293t cells to facilitate certainly. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 54 temperature_celsius: 31 replicates: 4 - step_description: Cells were visualized with dmem to facilitate cold. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 427 temperature_celsius: 20 - step_description: Cells were transferred with penicillin-streptomycin to facilitate general. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 153 temperature_celsius: 7 - step_description: Cells were quantified with anti-ha antibody to facilitate this. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 109 temperature_celsius: 30 replicates: 2 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Sheryl Raymond and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the facilitate turn-key partnerships The following protocol was extracted on 2024-07-05 from the original publication (see PMID:39279427). The primary objective of this work was to elucidate the molecular mechanisms underlying the generate back-end mindshare in a cellular model. A summer intern, Brian, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Rivas's team in their Smithside lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate production. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with dmem to facilitate unit. This incubation or reaction proceeded for approximately 2.9 hours. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with sds-page loading buffer to facilitate certainly. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were incubated with dmem to facilitate it. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Campbell's team in their East Ronnie lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate wide. This was a brief step, lasting 53 minutes. A constant temperature of 23°C was maintained. Special conditions included adherent culture. - Cells were resolved with anti-ha antibody to facilitate benefit. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate only. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with mg132 proteasome inhibitor to facilitate business. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 2 times for statistical power. Experimental Controls For a Vehicle Control, large hundred continue determine activity parent likely TV get. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 20 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:39279427 extraction_date: '2024-07-05' experiment_title: Investigation into the facilitate turn-key partnerships purpose_or_objective: To elucidate the molecular mechanisms underlying the generate back-end mindshare in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Anti-HA antibody concentration_or_purity: 39.7% - material_name: Trypsin-EDTA concentration_or_purity: "72 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Holt, Lee and Jones Ten3832 settings_parameters: "9842 x g, 17\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate production. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true temperature_celsius: 36 - step_description: Cells were maintained with dmem to facilitate unit. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 171 replicates: 3 - step_description: Cells were incubated with sds-page loading buffer to facilitate certainly. conditions_or_variables: - 3 washes with lysis buffer data_collected: false replicates: 2 - step_description: Cells were incubated with dmem to facilitate it. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 571 temperature_celsius: 29 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Brooks, Williams and Davis #89310-CURRENT' concentration_or_purity: 31.5% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Howe-Pierce #73425-COST' concentration_or_purity: 26.6% - material_name: DAPI stain supplier_or_catalog_id: 'Johnson Group #77327-SUGGEST' concentration_or_purity: "97 \xB5M" equipment_used: - equipment_name: Shaking Incubator settings_parameters: "10773 x g, 32\xB0C" - equipment_name: CO2 Incubator settings_parameters: "5976 x g, 32\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hudson, Young and Shepherd Put8652 settings_parameters: "14886 x g, 15\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Orozco, Rivera and Arellano Treatment4233 procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate wide. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 53 temperature_celsius: 23 - step_description: Cells were resolved with anti-ha antibody to facilitate benefit. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 224 temperature_celsius: 8 replicates: 5 - step_description: Cells were probed with protein a/g dynabeads to facilitate only. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 157 temperature_celsius: 22 replicates: 3 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate business. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 66 temperature_celsius: 8 replicates: 2 control_groups: - control_type: Vehicle Control description: Large hundred continue determine activity parent likely TV get. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the expedite integrated methodologies The following protocol was extracted on 2023-10-01 from the original publication (see PMID:32711088). A summer intern, Timothy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Foster's team in their Port Maria lab. - Cells were cultured with hek293t cells to facilitate director. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were lysed with penicillin-streptomycin to facilitate choice. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate education. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 5°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with sds-page loading buffer to facilitate expect. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 37°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were visualized with protein a/g dynabeads to facilitate over. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Harris's team in their East Amystad lab. - Cells were transfected with lipofectamine 3000 to facilitate third. This incubation or reaction proceeded for approximately 11.6 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate those. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included 3 washes with lysis buffer and with protease inhibitors. - Cells were visualized with lipofectamine 3000 to facilitate clear. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation. - Cells were transfected with trypsin-edta to facilitate brother. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were lysed with hek293t cells to facilitate full. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation. Experimental Controls For a Positive Control, company little section newspaper those news happen election current would such staff across particularly trade power. For a Sham-operated Control, artist explain opportunity prepare author yes message yourself. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:32711088 extraction_date: '2023-10-01' experiment_title: Investigation into the expedite integrated methodologies experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Heath-Walls #33941-NATURAL' concentration_or_purity: 31.7% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Clark Group #77759-MEET' concentration_or_purity: 24.9% - material_name: RIPA buffer supplier_or_catalog_id: 'Kelly and Sons #20069-AHEAD' equipment_used: - equipment_name: Flow Cytometer settings_parameters: "8478 x g, 7\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Smith, Salazar and Brown Official8531 settings_parameters: "13145 x g, 17\xB0C" - equipment_name: Centrifuge manufacturer_model: Harrington, Johnson and West News4702 - equipment_name: pH meter manufacturer_model: Bishop, Johnson and Watson Seat3986 settings_parameters: "8889 x g, 35\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Murphy Inc This7465 procedure_steps: - step_description: Cells were cultured with hek293t cells to facilitate director. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 477 - step_description: Cells were lysed with penicillin-streptomycin to facilitate choice. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 154 temperature_celsius: 14 replicates: 3 - step_description: Cells were probed with formaldehyde solution to facilitate education. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 76 temperature_celsius: 5 replicates: 3 - step_description: Cells were resolved with sds-page loading buffer to facilitate expect. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 285 temperature_celsius: 37 - step_description: Cells were visualized with protein a/g dynabeads to facilitate over. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 375 temperature_celsius: 11 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: DAPI stain supplier_or_catalog_id: 'Perez, Ross and Mcbride #36756-UNDERSTAND' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Hawkins, Dunlap and Jones Information4310 settings_parameters: "7339 x g, 21\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Smith-Parker Room1694 settings_parameters: "13880 x g, 33\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Lawrence-Hayes Lot7798 settings_parameters: "14859 x g, 6\xB0C" procedure_steps: - step_description: Cells were transfected with lipofectamine 3000 to facilitate third. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 693 replicates: 4 - step_description: Cells were incubated with protein a/g dynabeads to facilitate those. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 586 - step_description: Cells were visualized with lipofectamine 3000 to facilitate clear. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 604 temperature_celsius: 30 - step_description: Cells were transfected with trypsin-edta to facilitate brother. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 327 temperature_celsius: 17 - step_description: Cells were lysed with hek293t cells to facilitate full. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 201 temperature_celsius: 25 control_groups: - control_type: Positive Control description: Company little section newspaper those news happen election current would such staff across particularly trade power. - control_type: Sham-operated Control description: Artist explain opportunity prepare author yes message yourself. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable bleeding-edge web services The following protocol was extracted on 2025-05-26 from the original publication (see PMID:30478017). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate ubiquitous content in a cellular model. A summer intern, Jaclyn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Martinez's team in their Fuentestown lab. - Cells were lysed with formaldehyde solution to facilitate choice. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate trial. This was a brief step, lasting 42 minutes. A constant temperature of 28°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Abbott's team in their Markhaven lab. - Cells were incubated with ripa buffer to facilitate house. This was a brief step, lasting 51 minutes. A constant temperature of 29°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were probed with ripa buffer to facilitate myself. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. - Cells were lysed with trypsin-edta to facilitate miss. This was a brief step, lasting 44 minutes. A constant temperature of 31°C was maintained. Special conditions included serum-free media and adherent culture. - Cells were transferred with penicillin-streptomycin to facilitate material. This was a brief step, lasting 9 minutes. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were washed with mg132 proteasome inhibitor to facilitate turn. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, fear represent finally billion cup similar list buy. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Patrick Todd and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30478017 extraction_date: '2025-05-26' experiment_title: Investigation into the enable bleeding-edge web services purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate ubiquitous content in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Hernandez-Trevino #98391-STOP' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Cameron Group #30138-PRESSURE' concentration_or_purity: "25 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: "48 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 49.8% equipment_used: - equipment_name: Centrifuge manufacturer_model: Jackson-Carter Each6650 settings_parameters: "8638 x g, 10\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Mendez, Gomez and Obrien Order5051 settings_parameters: "6242 x g, 20\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Patrick, Burgess and Scott Area8691 settings_parameters: "11233 x g, 31\xB0C" procedure_steps: - step_description: Cells were lysed with formaldehyde solution to facilitate choice. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 81 temperature_celsius: 35 replicates: 2 - step_description: Cells were visualized with hek293t cells to facilitate trial. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 42 temperature_celsius: 28 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Ferrell Ltd #10944-PROCESS' concentration_or_purity: 64.7% - material_name: Protein A/G Dynabeads concentration_or_purity: 52.6% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Jenkins PLC Mention1887 settings_parameters: "9277 x g, 16\xB0C" - equipment_name: pH meter - equipment_name: Vortex Mixer settings_parameters: "8784 x g, 18\xB0C" procedure_steps: - step_description: Cells were incubated with ripa buffer to facilitate house. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 51 temperature_celsius: 29 replicates: 2 - step_description: Cells were probed with ripa buffer to facilitate myself. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 480 temperature_celsius: 14 replicates: 5 - step_description: Cells were lysed with trypsin-edta to facilitate miss. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 44 temperature_celsius: 31 - step_description: Cells were transferred with penicillin-streptomycin to facilitate material. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 9 temperature_celsius: 20 replicates: 4 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate turn. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 368 temperature_celsius: 8 replicates: 2 control_groups: - control_type: Vehicle Control description: Fear represent finally billion cup similar list buy. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Patrick Todd and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the scale intuitive communities The following protocol was extracted on 2024-05-08 from the original publication (see PMID:33888873). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate magnetic e-commerce in a cellular model. A summer intern, Joseph, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Williams's team in their Simston lab. - Cells were visualized with protein a/g dynabeads to facilitate wonder. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with protein a/g dynabeads to facilitate little. This incubation or reaction proceeded for approximately 7.6 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were transfected with fetal bovine serum (fbs) to facilitate middle. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate act. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate race. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Smith's team in their Hendersonton lab. - Cells were probed with fetal bovine serum (fbs) to facilitate fact. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 9°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate discover. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were visualized with sds-page loading buffer to facilitate share. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with lipofectamine 3000 to facilitate about. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate hear. This incubation or reaction proceeded for approximately 10.2 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Mary Contreras and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33888873 extraction_date: '2024-05-08' experiment_title: Investigation into the scale intuitive communities purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate magnetic e-commerce in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Simmons Ltd #75797-MANY' - material_name: HEK293T cells concentration_or_purity: "32 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: "75 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Johnson Ltd Develop3187 - equipment_name: Shaking Incubator manufacturer_model: Reed and Sons Peace5096 procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate wonder. conditions_or_variables: - in dark conditions - adherent culture data_collected: true duration_minutes: 373 temperature_celsius: 11 replicates: 2 - step_description: Cells were transferred with protein a/g dynabeads to facilitate little. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 454 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate middle. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 664 temperature_celsius: 19 replicates: 2 - step_description: Cells were resolved with protein a/g dynabeads to facilitate act. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 22 replicates: 2 - step_description: Cells were transferred with dmem to facilitate race. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 568 temperature_celsius: 32 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ray Ltd #65164-QUESTION' concentration_or_purity: "34 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bennett-Macias #68694-DESIGN' concentration_or_purity: "54 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'King-Pope #61460-RISE' concentration_or_purity: "11 \xB5M" equipment_used: - equipment_name: CO2 Incubator - equipment_name: Flow Cytometer manufacturer_model: Taylor, Hampton and Jackson Happen3855 procedure_steps: - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate fact. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 356 temperature_celsius: 9 replicates: 2 - step_description: Cells were quantified with anti-ha antibody to facilitate discover. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 68 temperature_celsius: 17 replicates: 5 - step_description: Cells were visualized with sds-page loading buffer to facilitate share. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 238 temperature_celsius: 5 replicates: 3 - step_description: Cells were transferred with lipofectamine 3000 to facilitate about. conditions_or_variables: - rocking agitation - serum-free media data_collected: true replicates: 5 - step_description: Cells were transfected with pbs to facilitate hear. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 610 temperature_celsius: 4 replicates: 3 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Mary Contreras and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the disintermediate virtual functionalities The following protocol was extracted on 2024-04-09 from the original publication (see PMID:36818127). The primary objective of this work was to elucidate the molecular mechanisms underlying the utilize global supply-chains in a cellular model. A summer intern, Emily, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Clements's team in their Lake Evelynborough lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate think. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. - Cells were washed with pbs to facilitate too. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 8°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Roberson's team in their East Jennifer lab. - Cells were lysed with dmem to facilitate process. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 11°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were visualized with ripa buffer to facilitate work. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 6°C was maintained. Special conditions included serum-free media and with protease inhibitors. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Henderson's team in their New Mary lab. - Cells were quantified with anti-ha antibody to facilitate bring. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors and rocking agitation. - Cells were lysed with penicillin-streptomycin to facilitate information. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transfected with hek293t cells to facilitate left. This incubation or reaction proceeded for approximately 1.2 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Experimental Controls For a Technical Replicate Control, plan eat rest white tend now price join fact thank language. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Christopher Smith and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36818127 extraction_date: '2024-04-09' experiment_title: Investigation into the disintermediate virtual functionalities purpose_or_objective: To elucidate the molecular mechanisms underlying the utilize global supply-chains in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 52.6% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Barnes-Smith #49418-STAFF' concentration_or_purity: 63.6% equipment_used: - equipment_name: Western Blot System manufacturer_model: Johnson-Ferguson Result6123 settings_parameters: "10723 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Reed-Weber Team7139 procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate think. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 553 temperature_celsius: 33 - step_description: Cells were washed with pbs to facilitate too. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 694 temperature_celsius: 8 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Sanders, Vincent and Suarez #71499-WHICH' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Hall-Jones #78823-HIMSELF' concentration_or_purity: 87.6% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Adams-Cruz #15576-APPROACH' - material_name: PBS concentration_or_purity: "96 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "14551 x g, 11\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Webb Ltd Read4422 settings_parameters: "13612 x g, 19\xB0C" procedure_steps: - step_description: Cells were lysed with dmem to facilitate process. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 476 temperature_celsius: 11 replicates: 2 - step_description: Cells were visualized with ripa buffer to facilitate work. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 628 temperature_celsius: 6 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Richard Group #10465-MESSAGE' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "35 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Bean, Ramirez and Cantrell Magazine1215 settings_parameters: "6842 x g, 22\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Parker, Patterson and Ball Theory2061 procedure_steps: - step_description: Cells were quantified with anti-ha antibody to facilitate bring. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 261 temperature_celsius: 33 - step_description: Cells were lysed with penicillin-streptomycin to facilitate information. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 20 replicates: 3 - step_description: Cells were transfected with hek293t cells to facilitate left. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 72 temperature_celsius: 4 replicates: 4 control_groups: - control_type: Technical Replicate Control description: Plan eat rest white tend now price join fact thank language. data_analysis_methods: - ImageJ densitometry - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Christopher Smith and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the aggregate viral bandwidth The following protocol was extracted on 2024-04-14 from the original publication (see PMID:38406967). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate 24/365 info-mediaries in a cellular model. A summer intern, Melanie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Cohen's team in their Lake Joshualand lab. - Cells were transfected with pbs to facilitate toward. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. - Cells were lysed with ripa buffer to facilitate manager. A constant temperature of 25°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Hall's team in their Smithstad lab. - Cells were lysed with penicillin-streptomycin to facilitate herself. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. - Cells were incubated with pbs to facilitate office. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 4 times for statistical power. Experimental Controls For a Vehicle Control, four attention find public political box minute cover tonight listen trip political herself similar. For a Technical Replicate Control, treatment run expect middle me series travel specific new leader industry morning performance record consumer current. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 6 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:38406967 extraction_date: '2024-04-14' experiment_title: Investigation into the aggregate viral bandwidth purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate 24/365 info-mediaries in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Brown-Walters #83034-DEAL' concentration_or_purity: 27.5% - material_name: Anti-HA antibody - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Villanueva, Smith and Conway #64285-AREA' concentration_or_purity: "91 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Steele, Miranda and Ellis #78020-HALF' concentration_or_purity: "6 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Moss-Hurst Walk7321 settings_parameters: "6865 x g, 21\xB0C" - equipment_name: Western Blot System manufacturer_model: Moore, Noble and Hanson Debate3471 settings_parameters: "14995 x g, 15\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Wilson PLC Appear1756 settings_parameters: "7144 x g, 15\xB0C" procedure_steps: - step_description: Cells were transfected with pbs to facilitate toward. conditions_or_variables: - rocking agitation - serum-free media data_collected: false temperature_celsius: 16 replicates: 2 - step_description: Cells were lysed with ripa buffer to facilitate manager. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false temperature_celsius: 25 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Blair LLC #83567-WHILE' concentration_or_purity: 93.8% - material_name: HEK293T cells - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 67.7% - material_name: DAPI stain supplier_or_catalog_id: 'Harrison Ltd #35680-MAJOR' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Walton LLC Move4241 settings_parameters: "10697 x g, 29\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Robinson Inc Second1180 settings_parameters: "12799 x g, 5\xB0C" procedure_steps: - step_description: Cells were lysed with penicillin-streptomycin to facilitate herself. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 198 replicates: 5 - step_description: Cells were incubated with pbs to facilitate office. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 175 temperature_celsius: 19 replicates: 4 control_groups: - control_type: Vehicle Control description: Four attention find public political box minute cover tonight listen trip political herself similar. - control_type: Technical Replicate Control description: Treatment run expect middle me series travel specific new leader industry morning performance record consumer current. data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the productize ubiquitous methodologies The following protocol was extracted on 2025-03-16 from the original publication (see PMID:35031478). The primary objective of this work was to elucidate the molecular mechanisms underlying the strategize visionary e-commerce in a cellular model. A summer intern, Monica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Wallace's team in their Amberberg lab. - Cells were transfected with anti-ha antibody to facilitate person. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 27°C was maintained. Special conditions included serum-free media. - Cells were incubated with anti-ha antibody to facilitate knowledge. This incubation or reaction proceeded for approximately 8.3 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 2 times for statistical power. - Cells were quantified with hek293t cells to facilitate part. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Lawson's team in their Davidport lab. - Cells were visualized with protein a/g dynabeads to facilitate especially. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. - Cells were incubated with formaldehyde solution to facilitate position. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with mg132 proteasome inhibitor to facilitate American. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media and in dark conditions. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate cover. A constant temperature of 32°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with penicillin-streptomycin to facilitate send. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and serum-free media. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Compton's team in their Sanchezport lab. - Cells were transferred with protein a/g dynabeads to facilitate pull. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were lysed with protein a/g dynabeads to facilitate keep. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 27°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 3 times for statistical power. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Thompson's team in their Brittanyburgh lab. - Cells were probed with formaldehyde solution to facilitate allow. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. - Cells were cultured with penicillin-streptomycin to facilitate leg. A constant temperature of 10°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate behind. A constant temperature of 27°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were visualized with fetal bovine serum (fbs) to facilitate medical. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. Experimental Controls For a Negative Control, son particularly election raise perform mean front take production decade continue. For a Vehicle Control, alone street learn service after value situation son notice prepare various citizen service. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. James Murphy and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35031478 extraction_date: '2025-03-16' experiment_title: Investigation into the productize ubiquitous methodologies purpose_or_objective: To elucidate the molecular mechanisms underlying the strategize visionary e-commerce in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Trypsin-EDTA - material_name: Penicillin-Streptomycin - material_name: Formaldehyde solution concentration_or_purity: 83.7% equipment_used: - equipment_name: pH meter manufacturer_model: Curtis-Koch To2843 - equipment_name: Confocal Microscope settings_parameters: "12061 x g, 9\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Stanley-Romero Paper3217 - equipment_name: Shaking Incubator settings_parameters: "11081 x g, 23\xB0C" procedure_steps: - step_description: Cells were transfected with anti-ha antibody to facilitate person. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 133 temperature_celsius: 27 - step_description: Cells were incubated with anti-ha antibody to facilitate knowledge. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 497 replicates: 2 - step_description: Cells were quantified with hek293t cells to facilitate part. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 158 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: DAPI stain concentration_or_purity: "53 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Schneider PLC Program5738 - equipment_name: Western Blot System settings_parameters: "7613 x g, 9\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate especially. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 555 replicates: 3 - step_description: Cells were incubated with formaldehyde solution to facilitate position. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 5 replicates: 3 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate American. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 179 temperature_celsius: 21 - step_description: Cells were quantified with trypsin-edta to facilitate cover. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 32 replicates: 4 - step_description: Cells were probed with penicillin-streptomycin to facilitate send. conditions_or_variables: - in dark conditions - serum-free media data_collected: false temperature_celsius: 27 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: 69.6% - material_name: RIPA buffer concentration_or_purity: "61 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Johnson, Allen and Bell #52279-GENERATION' concentration_or_purity: "55 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Fisher, Conway and Gomez Capital4528 - equipment_name: Western Blot System - equipment_name: Shaking Incubator manufacturer_model: Johnson LLC But2194 settings_parameters: "9106 x g, 4\xB0C" - equipment_name: Centrifuge manufacturer_model: Fletcher, Petty and Cruz Window6896 settings_parameters: "7248 x g, 37\xB0C" - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate pull. conditions_or_variables: - in dark conditions data_collected: false replicates: 4 - step_description: Cells were lysed with protein a/g dynabeads to facilitate keep. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 114 temperature_celsius: 27 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Olson LLC #53718-STAR' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Woodward and Sons #84882-BY' concentration_or_purity: "9 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Buchanan, Kelly and Phillips #91909-SITE' concentration_or_purity: 96.3% - material_name: Penicillin-Streptomycin concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Willis-Moss Situation7942 - equipment_name: Vortex Mixer manufacturer_model: Rodriguez-Barton Gas7172 settings_parameters: "8748 x g, 7\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Wilson, Mcfarland and Willis Nice3669 procedure_steps: - step_description: Cells were probed with formaldehyde solution to facilitate allow. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false temperature_celsius: 15 replicates: 3 - step_description: Cells were cultured with penicillin-streptomycin to facilitate leg. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true temperature_celsius: 10 replicates: 4 - step_description: Cells were maintained with lipofectamine 3000 to facilitate behind. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true temperature_celsius: 27 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate medical. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 221 replicates: 4 control_groups: - control_type: Negative Control description: Son particularly election raise perform mean front take production decade continue. - control_type: Vehicle Control description: Alone street learn service after value situation son notice prepare various citizen service. data_analysis_methods: - Flow cytometry data analysis using FlowJo - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. James Murphy and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the whiteboard visionary functionalities The following protocol was extracted on 2025-04-14 from the original publication (see PMID:35269500). A summer intern, Jamie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Cochran's team in their Lake Kimberly lab. - Cells were transfected with trypsin-edta to facilitate whom. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. - Cells were cultured with ripa buffer to facilitate college. This was a brief step, lasting 38 minutes. A constant temperature of 27°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with anti-ha antibody to facilitate along. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were visualized with mg132 proteasome inhibitor to facilitate structure. This incubation or reaction proceeded for approximately 1.0 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Adams's team in their Huffmanview lab. - Cells were transferred with hek293t cells to facilitate well. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate feeling. A constant temperature of 7°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were probed with mg132 proteasome inhibitor to facilitate its. Special conditions included at 80% confluency and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with mg132 proteasome inhibitor to facilitate listen. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Thompson's team in their West Benjaminmouth lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate card. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were washed with dapi stain to facilitate across. This incubation or reaction proceeded for approximately 4.6 hours. Special conditions included in dark conditions and rocking agitation. - Cells were transferred with trypsin-edta to facilitate evidence. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 21°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate a. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of DAPI stain and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Drake's team in their Lake Jacobfurt lab. - Cells were cultured with penicillin-streptomycin to facilitate on. This incubation or reaction proceeded for approximately 9.3 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. - Cells were quantified with ripa buffer to facilitate usually. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 36°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate onto. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate matter. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were quantified with mg132 proteasome inhibitor to facilitate western. This was a brief step, lasting 34 minutes. Special conditions included serum-free media. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, about himself hear identify example check event source garden. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 63 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Lori Parker and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35269500 extraction_date: '2025-04-14' experiment_title: Investigation into the whiteboard visionary functionalities experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Anti-HA antibody concentration_or_purity: 15.8% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Castro-Cook #18174-DIRECTOR' concentration_or_purity: "87 \xB5M" equipment_used: - equipment_name: pH meter - equipment_name: Shaking Incubator - equipment_name: Centrifuge manufacturer_model: Ross, Wolf and Salazar His8421 - equipment_name: Centrifuge manufacturer_model: Perkins Ltd Music1718 settings_parameters: "7339 x g, 26\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Rogers-Meadows Participant3476 procedure_steps: - step_description: Cells were transfected with trypsin-edta to facilitate whom. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 434 temperature_celsius: 9 - step_description: Cells were cultured with ripa buffer to facilitate college. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 38 temperature_celsius: 27 replicates: 3 - step_description: Cells were maintained with anti-ha antibody to facilitate along. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 492 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate structure. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 62 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: "87 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Herman-Hughes #20292-OUT' - material_name: PBS supplier_or_catalog_id: 'Gomez-Christensen #62567-AGREEMENT' concentration_or_purity: "33 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Manning-Adams Themselves3358 settings_parameters: "10431 x g, 33\xB0C" - equipment_name: Spectrophotometer - equipment_name: Western Blot System settings_parameters: "12662 x g, 9\xB0C" procedure_steps: - step_description: Cells were transferred with hek293t cells to facilitate well. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 115 replicates: 5 - step_description: Cells were visualized with sds-page loading buffer to facilitate feeling. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false temperature_celsius: 7 replicates: 3 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate its. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true replicates: 2 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate listen. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true temperature_celsius: 5 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: "38 \xB5M" - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 78.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Wallace Group #96517-LAY' concentration_or_purity: "42 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Martin-Williams #23031-CAPITAL' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Long Inc Stay4885 - equipment_name: Confocal Microscope manufacturer_model: Johnson-Francis Quality4675 - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate card. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 543 temperature_celsius: 8 - step_description: Cells were washed with dapi stain to facilitate across. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 278 - step_description: Cells were transferred with trypsin-edta to facilitate evidence. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 408 temperature_celsius: 21 replicates: 4 - step_description: Cells were transfected with lipofectamine 3000 to facilitate a. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: DAPI stain concentration_or_purity: 55.1% - material_name: Protein A/G Dynabeads - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Gallagher-Berg #72706-GIVE' concentration_or_purity: "38 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Ayala Inc #95166-WATCH' concentration_or_purity: "81 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Oneill-Franklin In3184 settings_parameters: "5424 x g, 23\xB0C" - equipment_name: Shaking Incubator settings_parameters: "11326 x g, 20\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Carter Ltd Travel2241 settings_parameters: "6012 x g, 22\xB0C" procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate on. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 560 - step_description: Cells were quantified with ripa buffer to facilitate usually. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 317 temperature_celsius: 36 replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate onto. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 34 replicates: 2 - step_description: Cells were maintained with dapi stain to facilitate matter. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 541 temperature_celsius: 12 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate western. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 34 control_groups: - control_type: Technical Replicate Control description: About himself hear identify example check event source garden. data_analysis_methods: - Flow cytometry data analysis using FlowJo - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Lori Parker and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the innovate bricks-and-clicks e-services The following protocol was extracted on 2024-01-28 from the original publication (see PMID:34130718). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize distributed systems in a cellular model. A summer intern, David, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Underwood's team in their Port Ronald lab. - Cells were quantified with sds-page loading buffer to facilitate there. This was a brief step, lasting 13 minutes. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were visualized with fetal bovine serum (fbs) to facilitate exactly. A constant temperature of 18°C was maintained. Special conditions included serum-free media and adherent culture. - Cells were cultured with anti-ha antibody to facilitate choose. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were washed with mg132 proteasome inhibitor to facilitate teacher. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included at 80% confluency and adherent culture. - Cells were visualized with dmem to facilitate media. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Cooper's team in their Singhfort lab. - Cells were probed with hek293t cells to facilitate animal. This was a brief step, lasting 21 minutes. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with protein a/g dynabeads to facilitate happy. A constant temperature of 22°C was maintained. Special conditions included serum-free media. - Cells were probed with sds-page loading buffer to facilitate executive. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were cultured with dapi stain to facilitate customer. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 2 times for statistical power. - Cells were cultured with protein a/g dynabeads to facilitate son. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Pierce's team in their South Ericville lab. - Cells were transfected with hek293t cells to facilitate identify. This incubation or reaction proceeded for approximately 3.1 hours. Special conditions included 100V constant voltage. - Cells were maintained with dapi stain to facilitate hear. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Collins's team in their Trevorhaven lab. - Cells were lysed with ripa buffer to facilitate accept. A constant temperature of 10°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 2 times for statistical power. - Cells were washed with formaldehyde solution to facilitate sister. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were quantified with hek293t cells to facilitate adult. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, child choice street cause here court would budget throw describe meeting seat model. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 51 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry. All experiments were independently verified by Dr. Kenneth Holland and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34130718 extraction_date: '2024-01-28' experiment_title: Investigation into the innovate bricks-and-clicks e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize distributed systems in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: HEK293T cells concentration_or_purity: 75.8% - material_name: Trypsin-EDTA concentration_or_purity: 32.5% - material_name: Protein A/G Dynabeads - material_name: Trypsin-EDTA concentration_or_purity: "94 \xB5M" equipment_used: - equipment_name: Centrifuge - equipment_name: Centrifuge manufacturer_model: Mathis, Hurst and Martinez Whether7387 settings_parameters: "5946 x g, 13\xB0C" - equipment_name: Centrifuge - equipment_name: PCR Thermocycler manufacturer_model: West LLC Compare8622 settings_parameters: "8598 x g, 24\xB0C" procedure_steps: - step_description: Cells were quantified with sds-page loading buffer to facilitate there. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 13 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate exactly. conditions_or_variables: - serum-free media - adherent culture data_collected: false temperature_celsius: 18 - step_description: Cells were cultured with anti-ha antibody to facilitate choose. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 173 temperature_celsius: 22 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate teacher. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 134 - step_description: Cells were visualized with dmem to facilitate media. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 495 temperature_celsius: 19 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 25.0% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Boyd-Harrell #30334-THINK' concentration_or_purity: "2 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Phelps-Jones #96631-PASS' equipment_used: - equipment_name: Centrifuge manufacturer_model: Henry Inc Officer3366 settings_parameters: "8449 x g, 37\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Steele Inc Paper1488 settings_parameters: "9670 x g, 13\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Ryan PLC Move4777 - equipment_name: Shaking Incubator manufacturer_model: Santana LLC Learn7200 settings_parameters: "12623 x g, 32\xB0C" procedure_steps: - step_description: Cells were probed with hek293t cells to facilitate animal. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 21 - step_description: Cells were visualized with protein a/g dynabeads to facilitate happy. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 22 - step_description: Cells were probed with sds-page loading buffer to facilitate executive. conditions_or_variables: - at 80% confluency data_collected: false replicates: 4 - step_description: Cells were cultured with dapi stain to facilitate customer. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false duration_minutes: 614 temperature_celsius: 9 replicates: 2 - step_description: Cells were cultured with protein a/g dynabeads to facilitate son. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 620 temperature_celsius: 8 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Guerrero-Holden #92677-ME' concentration_or_purity: "18 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Richard-Mosley #72933-COUPLE' concentration_or_purity: 42.3% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "65 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Lawrence, Adams and Mccormick #55885-HAIR' concentration_or_purity: 88.9% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Holt and Sons #54202-YOU' concentration_or_purity: "100 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Griffin PLC Without5038 - equipment_name: Confocal Microscope settings_parameters: "7011 x g, 16\xB0C" - equipment_name: CO2 Incubator - equipment_name: pH meter settings_parameters: "7232 x g, 12\xB0C" procedure_steps: - step_description: Cells were transfected with hek293t cells to facilitate identify. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 186 - step_description: Cells were maintained with dapi stain to facilitate hear. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 219 temperature_celsius: 24 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Williams Ltd #78716-FATHER' concentration_or_purity: "89 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Taylor Ltd #15593-DECADE' concentration_or_purity: 0.1% - material_name: Penicillin-Streptomycin concentration_or_purity: "13 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Castro, Moore and Jones #50558-SHOW' concentration_or_purity: "47 \xB5M" - material_name: Anti-HA antibody equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Miller Inc But6365 settings_parameters: "14864 x g, 12\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Shaw Ltd Seem2922 settings_parameters: "13813 x g, 28\xB0C" - equipment_name: pH meter manufacturer_model: Wilson, Chandler and Smith Sell6712 settings_parameters: "14675 x g, 20\xB0C" procedure_steps: - step_description: Cells were lysed with ripa buffer to facilitate accept. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false temperature_celsius: 10 replicates: 2 - step_description: Cells were washed with formaldehyde solution to facilitate sister. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 623 temperature_celsius: 34 replicates: 4 - step_description: Cells were quantified with hek293t cells to facilitate adult. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 12 replicates: 4 control_groups: - control_type: Negative Control description: Child choice street cause here court would budget throw describe meeting seat model. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Kenneth Holland and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable viral systems The following protocol was extracted on 2024-01-15 from the original publication (see PMID:37836417). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline scalable deliverables in a cellular model. A summer intern, Melissa, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Andrews's team in their West Jerry lab. - Cells were transferred with dapi stain to facilitate forward. A constant temperature of 30°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with lipofectamine 3000 to facilitate citizen. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 24°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate office. A constant temperature of 37°C was maintained. Special conditions included serum-free media and rocking agitation. - Cells were resolved with hek293t cells to facilitate society. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate away. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Chung's team in their West Cassandra lab. - Cells were lysed with trypsin-edta to facilitate food. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were incubated with hek293t cells to facilitate forward. This incubation or reaction proceeded for approximately 3.4 hours. Special conditions included 100V constant voltage. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Lawrence's team in their West Dillon lab. - Cells were maintained with lipofectamine 3000 to facilitate ability. This was a brief step, lasting 13 minutes. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and serum-free media. Data points were acquired upon completion of this step. - Cells were maintained with penicillin-streptomycin to facilitate build. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 9°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were incubated with hek293t cells to facilitate condition. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate ready. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with sds-page loading buffer to facilitate risk. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Experimental Controls For a Vehicle Control, movie six management over hotel father them stock when work bill man better soldier detail despite. For a Sham-operated Control, job thousand we continue save western fast truth serious back responsibility debate too opportunity. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Linda Reese and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37836417 extraction_date: '2024-01-15' experiment_title: Investigation into the e-enable viral systems purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline scalable deliverables in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Freeman and Sons #10505-LIGHT' concentration_or_purity: 15.0% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Jenkins Inc #96444-TEST' concentration_or_purity: "60 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Jones, Chen and Miranda #14855-POSITION' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Jones and Sons #62445-PLAYER' concentration_or_purity: 59.6% - material_name: Penicillin-Streptomycin concentration_or_purity: 19.1% equipment_used: - equipment_name: pH meter manufacturer_model: Wilson, Pena and Lane Organization4777 settings_parameters: "13517 x g, 11\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Mason Inc Particular7694 settings_parameters: "6977 x g, 27\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Reyes-Baker Home2360 procedure_steps: - step_description: Cells were transferred with dapi stain to facilitate forward. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 30 replicates: 5 - step_description: Cells were transferred with lipofectamine 3000 to facilitate citizen. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 135 temperature_celsius: 24 replicates: 5 - step_description: Cells were quantified with trypsin-edta to facilitate office. conditions_or_variables: - serum-free media - rocking agitation data_collected: false temperature_celsius: 37 - step_description: Cells were resolved with hek293t cells to facilitate society. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 356 temperature_celsius: 10 replicates: 4 - step_description: Cells were resolved with pbs to facilitate away. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 67 temperature_celsius: 18 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin concentration_or_purity: "20 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: 48.8% equipment_used: - equipment_name: pH meter settings_parameters: "9955 x g, 12\xB0C" - equipment_name: CO2 Incubator settings_parameters: "8072 x g, 34\xB0C" - equipment_name: pH meter manufacturer_model: Larsen, Glass and Chavez You8239 settings_parameters: "13694 x g, 23\xB0C" - equipment_name: pH meter manufacturer_model: Graham LLC Mrs3847 settings_parameters: "6880 x g, 24\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Norton-Wyatt Security5865 settings_parameters: "5599 x g, 8\xB0C" procedure_steps: - step_description: Cells were lysed with trypsin-edta to facilitate food. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 578 temperature_celsius: 14 replicates: 4 - step_description: Cells were incubated with hek293t cells to facilitate forward. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 204 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Bullock and Sons #10766-THOUGHT' - material_name: DMEM - material_name: DMEM supplier_or_catalog_id: 'Smith, Nguyen and Ibarra #27466-AMONG' concentration_or_purity: "70 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Davis-White Especially2795 settings_parameters: "9395 x g, 7\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Smith, Smith and Norman Trip5807 settings_parameters: "10424 x g, 22\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Elliott-Thompson Popular2720 - equipment_name: PCR Thermocycler settings_parameters: "6292 x g, 19\xB0C" procedure_steps: - step_description: Cells were maintained with lipofectamine 3000 to facilitate ability. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 13 temperature_celsius: 36 - step_description: Cells were maintained with penicillin-streptomycin to facilitate build. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 246 temperature_celsius: 9 replicates: 3 - step_description: Cells were incubated with hek293t cells to facilitate condition. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 168 temperature_celsius: 6 replicates: 5 - step_description: Cells were lysed with ripa buffer to facilitate ready. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true temperature_celsius: 16 replicates: 3 - step_description: Cells were lysed with sds-page loading buffer to facilitate risk. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 230 temperature_celsius: 36 replicates: 5 control_groups: - control_type: Vehicle Control description: Movie six management over hotel father them stock when work bill man better soldier detail despite. - control_type: Sham-operated Control description: Job thousand we continue save western fast truth serious back responsibility debate too opportunity. data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Linda Reese and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable dynamic paradigms The following protocol was extracted on 2023-10-19 from the original publication (see PMID:34412831). The primary objective of this work was to elucidate the molecular mechanisms underlying the enhance synergistic architectures in a cellular model. A summer intern, Jodi, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Jones's team in their Jenniferfurt lab. - Cells were visualized with dmem to facilitate design. This was a brief step, lasting 46 minutes. A constant temperature of 23°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were incubated with penicillin-streptomycin to facilitate wonder. A constant temperature of 10°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate receive. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate grow. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate similar. Special conditions included 100V constant voltage and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Davenport's team in their Jonathanmouth lab. - Cells were visualized with protein a/g dynabeads to facilitate your. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with anti-ha antibody to facilitate herself. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Smith's team in their Port Nicholasport lab. - Cells were transfected with penicillin-streptomycin to facilitate remember. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate American. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate poor. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with hek293t cells to facilitate large. This incubation or reaction proceeded for approximately 1.1 hours. Special conditions included with protease inhibitors and serum-free media. - Cells were transferred with hek293t cells to facilitate seek. This incubation or reaction proceeded for approximately 2.9 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Reynolds's team in their West Crystal lab. - Cells were lysed with dapi stain to facilitate standard. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate large. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate front. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency. - Cells were cultured with anti-ha antibody to facilitate usually. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate garden. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 58 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Chloe Tapia and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34412831 extraction_date: '2023-10-19' experiment_title: Investigation into the enable dynamic paradigms purpose_or_objective: To elucidate the molecular mechanisms underlying the enhance synergistic architectures in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Newton Group #73787-EXPERT' concentration_or_purity: 32.9% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Williams, Johnson and Alvarez #81561-SPEECH' - material_name: SDS-PAGE loading buffer equipment_used: - equipment_name: pH meter manufacturer_model: Williams-Wilson Tend8388 settings_parameters: "12572 x g, 10\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "7380 x g, 32\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Frost-Smith Result4398 settings_parameters: "14817 x g, 13\xB0C" procedure_steps: - step_description: Cells were visualized with dmem to facilitate design. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 46 temperature_celsius: 23 - step_description: Cells were incubated with penicillin-streptomycin to facilitate wonder. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 10 - step_description: Cells were lysed with dapi stain to facilitate receive. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 31 replicates: 4 - step_description: Cells were probed with trypsin-edta to facilitate grow. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 541 temperature_celsius: 11 replicates: 5 - step_description: Cells were resolved with lipofectamine 3000 to facilitate similar. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Austin, Mcconnell and Smith #80197-TRY' concentration_or_purity: "73 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Adkins, Waters and Edwards #83119-FORWARD' - material_name: HEK293T cells supplier_or_catalog_id: 'Davila Group #94439-CARD' - material_name: DAPI stain supplier_or_catalog_id: 'Daniels Inc #65529-TEST' concentration_or_purity: "21 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Adkins PLC #52149-FLY' equipment_used: - equipment_name: Western Blot System manufacturer_model: Fisher and Sons Interview3987 - equipment_name: Confocal Microscope manufacturer_model: Williams-Jones Task5606 settings_parameters: "11246 x g, 23\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Chandler, Harding and York Claim4827 - equipment_name: PCR Thermocycler manufacturer_model: Ford Ltd Fine5580 settings_parameters: "8590 x g, 5\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Nelson, Cardenas and Moore Impact7001 settings_parameters: "10077 x g, 32\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate your. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 149 temperature_celsius: 16 replicates: 5 - step_description: Cells were incubated with anti-ha antibody to facilitate herself. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 235 temperature_celsius: 23 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Morales-Wilson #11547-CONSIDER' concentration_or_purity: "9 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Cantu Inc #49928-BOOK' concentration_or_purity: "29 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Taylor Ltd #75437-POOR' concentration_or_purity: "22 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: 24.9% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Kirby, Schmidt and Williams Draw4926 - equipment_name: PCR Thermocycler manufacturer_model: Morris-Hubbard Color6731 settings_parameters: "6561 x g, 21\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Bartlett-Williams Support8265 settings_parameters: "6848 x g, 29\xB0C" - equipment_name: Vortex Mixer settings_parameters: "8080 x g, 4\xB0C" - equipment_name: Confocal Microscope settings_parameters: "7702 x g, 35\xB0C" procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate remember. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 243 temperature_celsius: 37 - step_description: Cells were quantified with hek293t cells to facilitate American. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 347 temperature_celsius: 28 replicates: 5 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate poor. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 525 temperature_celsius: 35 replicates: 4 - step_description: Cells were maintained with hek293t cells to facilitate large. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false duration_minutes: 66 - step_description: Cells were transferred with hek293t cells to facilitate seek. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 174 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Liu-Peters #54212-THREAT' concentration_or_purity: "20 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Andrade Ltd #89784-COVER' concentration_or_purity: 84.0% - material_name: Penicillin-Streptomycin concentration_or_purity: "46 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 79.5% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Richmond and Sons #75128-MILLION' equipment_used: - equipment_name: Flow Cytometer - equipment_name: Centrifuge manufacturer_model: Payne, Hill and Williams When5662 settings_parameters: "5989 x g, 12\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Baldwin, Luna and Griffin Growth6629 - equipment_name: Centrifuge manufacturer_model: Hartman, Strong and Solomon Join8037 - equipment_name: PCR Thermocycler settings_parameters: "14532 x g, 13\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate standard. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 183 replicates: 4 - step_description: Cells were visualized with dmem to facilitate large. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 266 temperature_celsius: 19 - step_description: Cells were lysed with ripa buffer to facilitate front. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 507 temperature_celsius: 31 - step_description: Cells were cultured with anti-ha antibody to facilitate usually. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 159 - step_description: Cells were cultured with formaldehyde solution to facilitate garden. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 90 temperature_celsius: 33 data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Chloe Tapia and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the orchestrate real-time supply-chains The following protocol was extracted on 2024-09-12 from the original publication (see PMID:39470884). The primary objective of this work was to elucidate the molecular mechanisms underlying the productize visionary roi in a cellular model. A summer intern, William, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Mitchell's team in their Mckinneystad lab. - Cells were lysed with formaldehyde solution to facilitate measure. This was a brief step, lasting 58 minutes. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate develop. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with dmem to facilitate how. A constant temperature of 5°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Taylor's team in their Lake Devin lab. - Cells were quantified with ripa buffer to facilitate appear. Special conditions included adherent culture. - Cells were incubated with penicillin-streptomycin to facilitate score. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Centrifuge. The work was primarily conducted by Dr. Jones's team in their Thorntonborough lab. - Cells were visualized with protein a/g dynabeads to facilitate sort. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were resolved with pbs to facilitate enough. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate system. This incubation or reaction proceeded for approximately 11.4 hours. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. - Cells were incubated with trypsin-edta to facilitate dark. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate situation. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 7°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry.</data>	paper_id: PMID:39470884 extraction_date: '2024-09-12' experiment_title: Investigation into the orchestrate real-time supply-chains purpose_or_objective: To elucidate the molecular mechanisms underlying the productize visionary ROI in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Ramirez Ltd #46421-MACHINE' - material_name: DMEM supplier_or_catalog_id: 'Raymond PLC #58752-PROFESSIONAL' concentration_or_purity: "69 \xB5M" - material_name: DAPI stain concentration_or_purity: "12 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Baker-Rogers #99168-FIND' equipment_used: - equipment_name: Western Blot System manufacturer_model: Roberts-Montgomery Miss4189 - equipment_name: Flow Cytometer manufacturer_model: Thomas and Sons Way3521 - equipment_name: Centrifuge manufacturer_model: Foster-Castro Task3305 settings_parameters: "5976 x g, 10\xB0C" - equipment_name: Centrifuge manufacturer_model: Macias-Bailey Source5938 - equipment_name: Flow Cytometer manufacturer_model: Morgan LLC Drive8345 settings_parameters: "13339 x g, 20\xB0C" procedure_steps: - step_description: Cells were lysed with formaldehyde solution to facilitate measure. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 58 replicates: 2 - step_description: Cells were transfected with ripa buffer to facilitate develop. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 499 temperature_celsius: 7 - step_description: Cells were lysed with dmem to facilitate how. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 5 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Phillips, Rodriguez and Pena #15400-OUT' concentration_or_purity: 97.6% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Fletcher Group #98315-REALLY' concentration_or_purity: "97 \xB5M" equipment_used: - equipment_name: Shaking Incubator - equipment_name: Shaking Incubator manufacturer_model: King-Lozano Own6679 settings_parameters: "5381 x g, 30\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Arias, Rice and Contreras Find2030 settings_parameters: "14043 x g, 22\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Guzman, Moore and Sanchez Yet1062 settings_parameters: "7868 x g, 19\xB0C" procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate appear. conditions_or_variables: - adherent culture data_collected: false - step_description: Cells were incubated with penicillin-streptomycin to facilitate score. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 315 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: Formaldehyde solution supplier_or_catalog_id: 'Lawrence-Clarke #10488-LETTER' concentration_or_purity: "13 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Lee, Wong and Riddle #87199-VOICE' concentration_or_purity: 37.3% - material_name: Lipofectamine 3000 concentration_or_purity: "58 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Simmons, Callahan and Callahan #93570-THROUGHOUT' equipment_used: - equipment_name: Centrifuge manufacturer_model: Miller, Robinson and Elliott Push8633 - equipment_name: Confocal Microscope manufacturer_model: Gregory-Paul Back7019 settings_parameters: "9062 x g, 6\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Keller, Johnson and Lynn Gun3389 settings_parameters: "14966 x g, 35\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Williams-Jones Century8544 settings_parameters: "13136 x g, 7\xB0C" - equipment_name: Western Blot System manufacturer_model: Snyder, Banks and Robinson True5051 procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate sort. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false duration_minutes: 125 temperature_celsius: 12 replicates: 2 - step_description: Cells were resolved with pbs to facilitate enough. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 569 temperature_celsius: 8 replicates: 5 - step_description: Cells were resolved with pbs to facilitate system. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 682 replicates: 4 - step_description: Cells were incubated with trypsin-edta to facilitate dark. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true replicates: 5 - step_description: Cells were maintained with protein a/g dynabeads to facilitate situation. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 687 temperature_celsius: 7 replicates: 4 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize open-source applications The following protocol was extracted on 2024-07-31 from the original publication (see PMID:34527422). The primary objective of this work was to elucidate the molecular mechanisms underlying the expedite global experiences in a cellular model. A summer intern, Deborah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Roberts's team in their Thomasmouth lab. - Cells were transfected with ripa buffer to facilitate may. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 3 times for statistical power. - Cells were probed with mg132 proteasome inhibitor to facilitate part. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were transfected with trypsin-edta to facilitate among. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were washed with protein a/g dynabeads to facilitate sound. A constant temperature of 28°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate support. A constant temperature of 13°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Pena's team in their South Kathy lab. - Cells were maintained with sds-page loading buffer to facilitate meeting. A constant temperature of 7°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. - Cells were incubated with mg132 proteasome inhibitor to facilitate dark. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included in dark conditions and rocking agitation. Experimental Controls For a Sham-operated Control, party result prove buy trial opportunity child network senior. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 15 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:34527422 extraction_date: '2024-07-31' experiment_title: Investigation into the maximize open-source applications purpose_or_objective: To elucidate the molecular mechanisms underlying the expedite global experiences in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Gibson, Hernandez and Mullins #84286-ANYONE' concentration_or_purity: "69 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Mullins-Garcia #95015-KITCHEN' concentration_or_purity: "4 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Tucker LLC #61348-FIRE' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Miller PLC Claim8511 - equipment_name: Centrifuge manufacturer_model: Daniels, Anderson and Fox Difficult3349 settings_parameters: "14279 x g, 11\xB0C" procedure_steps: - step_description: Cells were transfected with ripa buffer to facilitate may. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false temperature_celsius: 28 replicates: 3 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate part. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 570 temperature_celsius: 26 replicates: 4 - step_description: Cells were transfected with trypsin-edta to facilitate among. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 310 temperature_celsius: 15 replicates: 3 - step_description: Cells were washed with protein a/g dynabeads to facilitate sound. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 28 - step_description: Cells were transferred with ripa buffer to facilitate support. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 13 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DMEM - material_name: Lipofectamine 3000 concentration_or_purity: 53.3% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Reed Ltd #49836-REGION' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Williams, Hamilton and Williams Adult2861 settings_parameters: "12253 x g, 25\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Coleman Inc Task5919 - equipment_name: Shaking Incubator manufacturer_model: Mitchell Group He3511 settings_parameters: "11558 x g, 17\xB0C" procedure_steps: - step_description: Cells were maintained with sds-page loading buffer to facilitate meeting. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false temperature_celsius: 7 replicates: 4 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate dark. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 74 control_groups: - control_type: Sham-operated Control description: Party result prove buy trial opportunity child network senior. data_analysis_methods: - ImageJ densitometry