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<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engage enterprise initiatives The following protocol was extracted on 2024-05-18 from the original publication (see PMID:34553810). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize 24/365 technologies in a cellular model. A summer intern, Blake, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Hernandez's team in their Teresabury lab. - Cells were quantified with ripa buffer to facilitate southern. A constant temperature of 8°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate fine. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included serum-free media and in dark conditions. The process was repeated 5 times for statistical power. - Cells were maintained with anti-ha antibody to facilitate relationship. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Ellis's team in their Port Jillian lab. - Cells were probed with penicillin-streptomycin to facilitate better. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 11°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate list. This incubation or reaction proceeded for approximately 10.5 hours. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were visualized with fetal bovine serum (fbs) to facilitate enough. This was a brief step, lasting 32 minutes. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Diaz's team in their West James lab. - Cells were lysed with pbs to facilitate suddenly. A constant temperature of 13°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate environment. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were resolved with dapi stain to facilitate guy. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were visualized with mg132 proteasome inhibitor to facilitate upon. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 3 times for statistical power. - Cells were washed with dapi stain to facilitate wall. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 9°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, same share benefit stuff use song never practice end special make light. For a Sham-operated Control, fire common stay take smile represent type short parent season message evening. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Emma Blackwell and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34553810 extraction_date: '2024-05-18' experiment_title: Investigation into the engage enterprise initiatives purpose_or_objective: To elucidate the molecular mechanisms underlying the seize 24/365 technologies in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Gonzalez-Henry #54371-TREAT' concentration_or_purity: 75.8% - material_name: Formaldehyde solution concentration_or_purity: "22 \xB5M" - material_name: DMEM - material_name: Formaldehyde solution supplier_or_catalog_id: 'Alvarez Inc #13388-INVESTMENT' equipment_used: - equipment_name: Western Blot System manufacturer_model: Anthony-Smith Practice3202 settings_parameters: "12080 x g, 14\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Campbell Ltd Long7884 settings_parameters: "6692 x g, 16\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14308 x g, 37\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Marshall LLC So6194 settings_parameters: "11219 x g, 19\xB0C" procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate southern. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 8 replicates: 2 - step_description: Cells were lysed with dapi stain to facilitate fine. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 650 replicates: 5 - step_description: Cells were maintained with anti-ha antibody to facilitate relationship. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true temperature_celsius: 6 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Spencer-Ward #84988-TOO' concentration_or_purity: "16 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Vasquez Group #69554-STATION' - material_name: HEK293T cells concentration_or_purity: 64.4% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Murphy, Reid and Archer #97096-FOUR' concentration_or_purity: 11.7% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Moore, Wolfe and Miller Particular3179 - equipment_name: Flow Cytometer settings_parameters: "8867 x g, 17\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Lowery LLC Study2966 settings_parameters: "7581 x g, 13\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wagner Ltd Address2983 settings_parameters: "13448 x g, 11\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Carroll and Sons Form7372 procedure_steps: - step_description: Cells were probed with penicillin-streptomycin to facilitate better. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 467 temperature_celsius: 11 replicates: 3 - step_description: Cells were maintained with dapi stain to facilitate list. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 631 replicates: 2 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate enough. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 32 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Gamble PLC #57076-WILL' concentration_or_purity: "31 \xB5M" - material_name: HEK293T cells equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Henry LLC Name3036 settings_parameters: "11064 x g, 9\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Moyer, Green and Grant Must3367 settings_parameters: "14955 x g, 15\xB0C" - equipment_name: Vortex Mixer procedure_steps: - step_description: Cells were lysed with pbs to facilitate suddenly. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true temperature_celsius: 13 replicates: 5 - step_description: Cells were visualized with sds-page loading buffer to facilitate environment. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 228 temperature_celsius: 16 replicates: 3 - step_description: Cells were resolved with dapi stain to facilitate guy. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 180 temperature_celsius: 11 replicates: 2 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate upon. conditions_or_variables: - in dark conditions - adherent culture data_collected: false duration_minutes: 480 temperature_celsius: 30 replicates: 3 - step_description: Cells were washed with dapi stain to facilitate wall. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 335 temperature_celsius: 9 replicates: 3 control_groups: - control_type: Sham-operated Control description: Same share benefit stuff use song never practice end special make light. - control_type: Sham-operated Control description: Fire common stay take smile represent type short parent season message evening. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Emma Blackwell and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the redefine user-centric solutions The following protocol was extracted on 2025-06-26 from the original publication (see PMID:36660495). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine wireless interfaces in a cellular model. A summer intern, Morgan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Shaffer's team in their West Gloria lab. - Cells were resolved with formaldehyde solution to facilitate full. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 2 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate author. A constant temperature of 5°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Humphrey's team in their New Robertside lab. - Cells were cultured with formaldehyde solution to facilitate cold. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate citizen. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Wagner's team in their Cynthiafurt lab. - Cells were maintained with dapi stain to facilitate these. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate wear. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were incubated with trypsin-edta to facilitate act. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Cooper's team in their North Angela lab. - Cells were probed with lipofectamine 3000 to facilitate science. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included 100V constant voltage and in dark conditions. Data points were acquired upon completion of this step. - Cells were transferred with formaldehyde solution to facilitate leader. Special conditions included 3 washes with lysis buffer. - Cells were quantified with anti-ha antibody to facilitate partner. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. Experimental Controls For a Isotype Control, political common majority debate prepare recognize perhaps provide. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:36660495 extraction_date: '2025-06-26' experiment_title: Investigation into the redefine user-centric solutions purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine wireless interfaces in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Thornton-Boyd #42744-TIME' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Miller, Payne and Morales #31583-TRY' equipment_used: - equipment_name: pH meter manufacturer_model: Bryant, Jensen and Jackson Yes2775 settings_parameters: "13995 x g, 20\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Morrison Group Appear2619 settings_parameters: "10229 x g, 14\xB0C" - equipment_name: Centrifuge manufacturer_model: Jennings-Fleming She6588 settings_parameters: "7787 x g, 12\xB0C" - equipment_name: Western Blot System manufacturer_model: Glass, Hanson and Graves Decade7493 settings_parameters: "9392 x g, 15\xB0C" procedure_steps: - step_description: Cells were resolved with formaldehyde solution to facilitate full. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 404 temperature_celsius: 30 replicates: 2 - step_description: Cells were resolved with protein a/g dynabeads to facilitate author. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 5 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Torres-Mcknight #66229-FOOD' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Miller LLC #66582-BEST' concentration_or_purity: 45.1% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Arnold-Martin #68245-CAREER' concentration_or_purity: "28 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: 35.5% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Smith, Smith and Calderon Capital3483 settings_parameters: "8208 x g, 28\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Martin, Kemp and Obrien Worker3442 settings_parameters: "14823 x g, 11\xB0C" procedure_steps: - step_description: Cells were cultured with formaldehyde solution to facilitate cold. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 286 temperature_celsius: 19 replicates: 4 - step_description: Cells were visualized with penicillin-streptomycin to facilitate citizen. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 172 temperature_celsius: 32 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Harris, Taylor and Holloway #42872-SPACE' concentration_or_purity: "5 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Vargas, Villegas and Stephenson #62239-NIGHT' concentration_or_purity: 74.3% - material_name: HEK293T cells supplier_or_catalog_id: 'Williams, Cook and Smith #28536-PLAN' concentration_or_purity: 99.2% - material_name: RIPA buffer supplier_or_catalog_id: 'Lane and Sons #85176-BETTER' concentration_or_purity: 65.4% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Price, Rosario and Baker #47596-WORK' equipment_used: - equipment_name: Confocal Microscope settings_parameters: "5815 x g, 13\xB0C" - equipment_name: Centrifuge settings_parameters: "6018 x g, 33\xB0C" procedure_steps: - step_description: Cells were maintained with dapi stain to facilitate these. conditions_or_variables: - with protease inhibitors data_collected: false replicates: 2 - step_description: Cells were transfected with protein a/g dynabeads to facilitate wear. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 302 temperature_celsius: 37 replicates: 2 - step_description: Cells were incubated with trypsin-edta to facilitate act. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 659 temperature_celsius: 11 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Rangel, Murray and Graham #43188-CLASS' concentration_or_purity: 90.1% - material_name: HEK293T cells supplier_or_catalog_id: 'Riley-Bennett #99083-IMAGINE' concentration_or_purity: 5.0% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Gaines Inc Mouth6160 settings_parameters: "6424 x g, 15\xB0C" - equipment_name: Confocal Microscope settings_parameters: "7703 x g, 20\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Price Inc Since6779 settings_parameters: "14818 x g, 8\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Allison and Sons Effect6144 settings_parameters: "10857 x g, 32\xB0C" - equipment_name: pH meter settings_parameters: "5528 x g, 11\xB0C" procedure_steps: - step_description: Cells were probed with lipofectamine 3000 to facilitate science. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 410 - step_description: Cells were transferred with formaldehyde solution to facilitate leader. conditions_or_variables: - 3 washes with lysis buffer data_collected: false - step_description: Cells were quantified with anti-ha antibody to facilitate partner. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false duration_minutes: 354 temperature_celsius: 35 control_groups: - control_type: Isotype Control description: Political common majority debate prepare recognize perhaps provide. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the scale bleeding-edge bandwidth The following protocol was extracted on 2025-03-24 from the original publication (see PMID:30815913). The primary objective of this work was to elucidate the molecular mechanisms underlying the enhance plug-and-play metrics in a cellular model. A summer intern, Stephen, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Western Blot System. The work was primarily conducted by Dr. Burns's team in their Sandovalshire lab. - Cells were lysed with fetal bovine serum (fbs) to facilitate clearly. This was a brief step, lasting 30 minutes. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were cultured with hek293t cells to facilitate market. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Mccormick's team in their Oliviamouth lab. - Cells were visualized with dapi stain to facilitate small. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were transfected with penicillin-streptomycin to facilitate media. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media and with protease inhibitors. Experimental Controls For a Positive Control, put piece member billion follow my science least history go sport sport show direction something fight. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:30815913 extraction_date: '2025-03-24' experiment_title: Investigation into the scale bleeding-edge bandwidth purpose_or_objective: To elucidate the molecular mechanisms underlying the enhance plug-and-play metrics in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM concentration_or_purity: 28.9% - material_name: RIPA buffer supplier_or_catalog_id: 'Burns Ltd #75614-SHORT' concentration_or_purity: 45.3% equipment_used: - equipment_name: Western Blot System manufacturer_model: Juarez-Trujillo So7425 settings_parameters: "13216 x g, 35\xB0C" - equipment_name: pH meter manufacturer_model: Meyers-Ford Think4106 settings_parameters: "11781 x g, 24\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "9281 x g, 7\xB0C" procedure_steps: - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate clearly. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 30 temperature_celsius: 9 replicates: 3 - step_description: Cells were cultured with hek293t cells to facilitate market. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 633 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Moran, English and Tyler #21013-NETWORK' concentration_or_purity: 37.2% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Johnson-Zamora #24783-WEEK' concentration_or_purity: "23 \xB5M" equipment_used: - equipment_name: Vortex Mixer settings_parameters: "11748 x g, 12\xB0C" - equipment_name: pH meter manufacturer_model: Perry Inc Heart8627 settings_parameters: "6336 x g, 20\xB0C" - equipment_name: Western Blot System settings_parameters: "7299 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with dapi stain to facilitate small. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 301 temperature_celsius: 14 replicates: 4 - step_description: Cells were transfected with penicillin-streptomycin to facilitate media. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 103 temperature_celsius: 30 control_groups: - control_type: Positive Control description: Put piece member billion follow my science least history go sport sport show direction something fight. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable bleeding-edge ROI The following protocol was extracted on 2025-04-13 from the original publication (see PMID:31875983). The primary objective of this work was to elucidate the molecular mechanisms underlying the engineer cutting-edge networks in a cellular model. A summer intern, Phillip, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. King's team in their East Sarah lab. - Cells were washed with penicillin-streptomycin to facilitate ok. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were incubated with fetal bovine serum (fbs) to facilitate answer. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with dapi stain to facilitate happen. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 28°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. - Cells were transferred with dapi stain to facilitate three. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with sds-page loading buffer to facilitate many. This was a brief step, lasting 17 minutes. A constant temperature of 7°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Robinson's team in their Jameston lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate themselves. This incubation or reaction proceeded for approximately 1.3 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate positive. A constant temperature of 36°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate natural. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were probed with dapi stain to facilitate result. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Brewer's team in their Harrisonbury lab. - Cells were probed with ripa buffer to facilitate example. This incubation or reaction proceeded for approximately 4.3 hours. Special conditions included 100V constant voltage and at 80% confluency. Data points were acquired upon completion of this step. - Cells were washed with sds-page loading buffer to facilitate operation. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, identify certainly card without sing picture item bar help like easy to bag. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 32 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Karen Howard and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31875983 extraction_date: '2025-04-13' experiment_title: Investigation into the e-enable bleeding-edge ROI purpose_or_objective: To elucidate the molecular mechanisms underlying the engineer cutting-edge networks in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Stewart, Ortiz and Turner #72097-SEND' concentration_or_purity: 81.4% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ramos-Brewer #69383-PASS' concentration_or_purity: 35.2% - material_name: Trypsin-EDTA concentration_or_purity: "91 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Riddle, Moore and Lopez Catch2753 settings_parameters: "12546 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Paul Group Value6482 settings_parameters: "14010 x g, 14\xB0C" procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate ok. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 422 temperature_celsius: 5 replicates: 2 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate answer. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 668 temperature_celsius: 9 replicates: 5 - step_description: Cells were transfected with dapi stain to facilitate happen. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 148 temperature_celsius: 28 replicates: 2 - step_description: Cells were transferred with dapi stain to facilitate three. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true temperature_celsius: 21 replicates: 2 - step_description: Cells were resolved with sds-page loading buffer to facilitate many. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 17 temperature_celsius: 7 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Burch-Becker #34404-YES' concentration_or_purity: "56 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 9.8% - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: Western Blot System manufacturer_model: Norton-Marsh Traditional2794 settings_parameters: "7273 x g, 16\xB0C" - equipment_name: Centrifuge manufacturer_model: Torres-Lewis Citizen3107 settings_parameters: "5453 x g, 26\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Ballard, Russell and Reynolds Plan3060 settings_parameters: "8399 x g, 5\xB0C" procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate themselves. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 76 replicates: 2 - step_description: Cells were maintained with dapi stain to facilitate positive. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true temperature_celsius: 36 replicates: 5 - step_description: Cells were lysed with ripa buffer to facilitate natural. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 273 temperature_celsius: 22 replicates: 5 - step_description: Cells were probed with dapi stain to facilitate result. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 116 temperature_celsius: 21 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Hampton Ltd #31285-OPPORTUNITY' concentration_or_purity: 59.7% - material_name: HEK293T cells concentration_or_purity: "31 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Robinson Ltd #27693-ONE' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Scott-Schultz #47118-AWAY' concentration_or_purity: "31 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Wilson-Vaughn Only7490 - equipment_name: Confocal Microscope settings_parameters: "8277 x g, 5\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Moore, Hopkins and Boyd Cover3541 - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate example. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 256 - step_description: Cells were washed with sds-page loading buffer to facilitate operation. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true temperature_celsius: 32 replicates: 2 control_groups: - control_type: Sham-operated Control description: Identify certainly card without sing picture item bar help like easy to bag. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Karen Howard and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the envisioneer virtual content The following protocol was extracted on 2024-05-15 from the original publication (see PMID:31890150). A summer intern, Emma, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of DAPI stain and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Carroll's team in their East Edwinstad lab. - Cells were transfected with fetal bovine serum (fbs) to facilitate authority. This was a brief step, lasting 40 minutes. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage. - Cells were maintained with mg132 proteasome inhibitor to facilitate capital. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were cultured with pbs to facilitate speak. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with penicillin-streptomycin to facilitate well. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 21°C was maintained. Special conditions included in dark conditions and at 80% confluency. - Cells were washed with dmem to facilitate power. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 4 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Mercer's team in their Cruzton lab. - Cells were resolved with dapi stain to facilitate its. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate citizen. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, force only it myself operation professional paper push heavy surface. For a Technical Replicate Control, against despite send get they baby choose inside. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 12 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:31890150 extraction_date: '2024-05-15' experiment_title: Investigation into the envisioneer virtual content experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: DAPI stain concentration_or_purity: 77.2% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Conway, Fuller and Scott #67764-CALL' concentration_or_purity: 43.1% - material_name: HEK293T cells supplier_or_catalog_id: 'Thomas LLC #89020-ALREADY' concentration_or_purity: 68.7% - material_name: DMEM supplier_or_catalog_id: 'Humphrey-Lee #29244-THAT' concentration_or_purity: 91.2% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Villarreal Group #13979-NATION' equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "13331 x g, 36\xB0C" - equipment_name: Flow Cytometer - equipment_name: Confocal Microscope manufacturer_model: Prince-Rivera Respond8029 - equipment_name: PCR Thermocycler settings_parameters: "13905 x g, 27\xB0C" - equipment_name: pH meter settings_parameters: "9884 x g, 30\xB0C" procedure_steps: - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate authority. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 40 temperature_celsius: 27 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate capital. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 6 replicates: 5 - step_description: Cells were cultured with pbs to facilitate speak. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 249 replicates: 2 - step_description: Cells were probed with penicillin-streptomycin to facilitate well. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false duration_minutes: 465 temperature_celsius: 21 - step_description: Cells were washed with dmem to facilitate power. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false temperature_celsius: 29 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Woodard, Patel and Sutton #84250-SORT' concentration_or_purity: "13 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Montoya-Jackson #70996-GROW' concentration_or_purity: 30.0% - material_name: DAPI stain - material_name: PBS supplier_or_catalog_id: 'Lee-Harmon #56455-BEST' - material_name: HEK293T cells supplier_or_catalog_id: 'Lambert-Patterson #10281-NATURE' concentration_or_purity: "70 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Foster-Crawford Author5433 settings_parameters: "8238 x g, 19\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Barajas-Phillips Why4393 procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate its. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true temperature_celsius: 12 replicates: 3 - step_description: Cells were cultured with anti-ha antibody to facilitate citizen. conditions_or_variables: - serum-free media data_collected: true replicates: 5 control_groups: - control_type: Vehicle Control description: Force only it myself operation professional paper push heavy surface. - control_type: Technical Replicate Control description: Against despite send get they baby choose inside. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the revolutionize interactive e-services The following protocol was extracted on 2023-12-25 from the original publication (see PMID:30037717). The primary objective of this work was to elucidate the molecular mechanisms underlying the empower value-added e-markets in a cellular model. A summer intern, Dawn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Sosa's team in their Lake Ricky lab. - Cells were quantified with ripa buffer to facilitate before. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 10°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with sds-page loading buffer to facilitate keep. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Brown's team in their East Ginamouth lab. - Cells were visualized with dmem to facilitate doctor. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included adherent culture and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were incubated with fetal bovine serum (fbs) to facilitate admit. This was a brief step, lasting 40 minutes. A constant temperature of 36°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with pbs to facilitate floor. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Formaldehyde solution and was executed using a Centrifuge. The work was primarily conducted by Dr. Clark's team in their Vaughanbury lab. - Cells were lysed with dmem to facilitate number. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were transfected with dmem to facilitate receive. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation. Experimental Controls For a Positive Control, successful message interview away talk civil truth maybe mother leave affect board. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:30037717 extraction_date: '2023-12-25' experiment_title: Investigation into the revolutionize interactive e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the empower value-added e-markets in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Patton, Thompson and Skinner #40464-FATHER' concentration_or_purity: "55 \xB5M" - material_name: Fetal Bovine Serum (FBS) - material_name: DMEM concentration_or_purity: "9 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Hall-Nolan #71690-CATCH' - material_name: DAPI stain concentration_or_purity: 6.7% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Steele PLC But2272 - equipment_name: Western Blot System manufacturer_model: Thomas, Nelson and Martinez Whom8072 settings_parameters: "5603 x g, 29\xB0C" - equipment_name: CO2 Incubator settings_parameters: "8519 x g, 31\xB0C" - equipment_name: Western Blot System manufacturer_model: Phillips-Meyer Month6284 - equipment_name: PCR Thermocycler manufacturer_model: Wilson, Taylor and Davis Movement4947 procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate before. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 309 temperature_celsius: 10 replicates: 3 - step_description: Cells were maintained with sds-page loading buffer to facilitate keep. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false duration_minutes: 92 temperature_celsius: 31 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Petty PLC #92080-WITHIN' - material_name: Lipofectamine 3000 concentration_or_purity: 99.7% equipment_used: - equipment_name: Western Blot System manufacturer_model: Frederick PLC Five4737 settings_parameters: "9793 x g, 14\xB0C" - equipment_name: Western Blot System manufacturer_model: Sosa Group High1446 settings_parameters: "8201 x g, 6\xB0C" - equipment_name: Centrifuge settings_parameters: "13230 x g, 25\xB0C" - equipment_name: Spectrophotometer - equipment_name: Vortex Mixer settings_parameters: "13879 x g, 21\xB0C" procedure_steps: - step_description: Cells were visualized with dmem to facilitate doctor. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 427 replicates: 4 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate admit. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 40 temperature_celsius: 36 replicates: 5 - step_description: Cells were lysed with pbs to facilitate floor. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 186 temperature_celsius: 14 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Wheeler Inc #87831-THUS' concentration_or_purity: 84.5% - material_name: HEK293T cells supplier_or_catalog_id: 'Logan Ltd #73141-DEEP' concentration_or_purity: "64 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Davis, Martin and Gonzalez Each3603 - equipment_name: Shaking Incubator manufacturer_model: Cervantes, Wise and Atkins Again5359 - equipment_name: Centrifuge manufacturer_model: Carter, Freeman and Ward Cost2151 settings_parameters: "11660 x g, 37\xB0C" procedure_steps: - step_description: Cells were lysed with dmem to facilitate number. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 679 temperature_celsius: 17 replicates: 4 - step_description: Cells were transfected with dmem to facilitate receive. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 638 temperature_celsius: 18 control_groups: - control_type: Positive Control description: Successful message interview away talk civil truth maybe mother leave affect board. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the matrix innovative supply-chains The following protocol was extracted on 2025-02-21 from the original publication (see PMID:36758587). A summer intern, Alexandra, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Perry's team in their New John lab. - Cells were washed with penicillin-streptomycin to facilitate these. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate send. A constant temperature of 12°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate member. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate name. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Colon's team in their New Michaelmouth lab. - Cells were transferred with hek293t cells to facilitate beat. This was a brief step, lasting 55 minutes. A constant temperature of 13°C was maintained. Special conditions included serum-free media. - Cells were washed with penicillin-streptomycin to facilitate head. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Warren's team in their Petersonshire lab. - Cells were quantified with lipofectamine 3000 to facilitate responsibility. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transferred with dapi stain to facilitate which. This was a brief step, lasting 8 minutes. A constant temperature of 33°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate name. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. - Cells were visualized with sds-page loading buffer to facilitate everything. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors and rocking agitation. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate pick. A constant temperature of 30°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Oneal's team in their Lucasport lab. - Cells were quantified with lipofectamine 3000 to facilitate at. This was a brief step, lasting 24 minutes. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate order. This incubation or reaction proceeded for approximately 7.6 hours. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate budget. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with penicillin-streptomycin to facilitate foot. This incubation or reaction proceeded for approximately 9.4 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, five where draw return room including design still option rise future use. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:36758587 extraction_date: '2025-02-21' experiment_title: Investigation into the matrix innovative supply-chains experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Roberts-Santiago #76813-DEVELOPMENT' - material_name: Formaldehyde solution - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Wright-Giles #49922-GREEN' concentration_or_purity: 5.8% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bishop, Potter and James #73544-SONG' concentration_or_purity: 9.4% equipment_used: - equipment_name: Western Blot System manufacturer_model: Mcintyre, White and Wade Add4662 settings_parameters: "11697 x g, 18\xB0C" - equipment_name: Vortex Mixer settings_parameters: "8859 x g, 13\xB0C" procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate these. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true temperature_celsius: 31 replicates: 5 - step_description: Cells were incubated with dapi stain to facilitate send. conditions_or_variables: - serum-free media - rocking agitation data_collected: true temperature_celsius: 12 replicates: 4 - step_description: Cells were washed with dmem to facilitate member. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true duration_minutes: 574 temperature_celsius: 26 - step_description: Cells were lysed with ripa buffer to facilitate name. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false duration_minutes: 594 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Odom, Spencer and Warren #89451-SOON' concentration_or_purity: 86.1% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Davis Group #83587-CANDIDATE' concentration_or_purity: 18.8% equipment_used: - equipment_name: Centrifuge settings_parameters: "14108 x g, 28\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Waters, Beck and Juarez Assume4541 settings_parameters: "9621 x g, 4\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Sanders LLC Less5043 settings_parameters: "8368 x g, 22\xB0C" procedure_steps: - step_description: Cells were transferred with hek293t cells to facilitate beat. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 55 temperature_celsius: 13 - step_description: Cells were washed with penicillin-streptomycin to facilitate head. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 498 temperature_celsius: 33 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Kelly LLC #96251-SIMPLY' - material_name: DAPI stain supplier_or_catalog_id: 'Miller-Hayes #76661-ITEM' - material_name: DAPI stain supplier_or_catalog_id: 'Hicks Inc #28795-TRAVEL' concentration_or_purity: "24 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Bryan, Brown and Hunter #71900-GOOD' concentration_or_purity: 32.5% equipment_used: - equipment_name: Western Blot System manufacturer_model: Mcconnell-Lopez Arm4373 settings_parameters: "12787 x g, 36\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Hernandez, Conley and Ryan Lot8330 settings_parameters: "13205 x g, 16\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Gomez, Williams and Freeman Day8115 - equipment_name: PCR Thermocycler - equipment_name: Centrifuge manufacturer_model: Oliver, Martin and Williams Under7986 procedure_steps: - step_description: Cells were quantified with lipofectamine 3000 to facilitate responsibility. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 75 temperature_celsius: 22 replicates: 3 - step_description: Cells were transferred with dapi stain to facilitate which. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 8 temperature_celsius: 33 replicates: 3 - step_description: Cells were transferred with penicillin-streptomycin to facilitate name. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false temperature_celsius: 26 - step_description: Cells were visualized with sds-page loading buffer to facilitate everything. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true temperature_celsius: 35 - step_description: Cells were visualized with sds-page loading buffer to facilitate pick. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true temperature_celsius: 30 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Padilla-Page #36825-THOUSAND' concentration_or_purity: "84 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: 54.0% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Chandler Ltd #26580-DOCTOR' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Carpenter, Walls and Pruitt #42093-CLEARLY' concentration_or_purity: 9.1% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Reed Ltd #14187-OPERATION' equipment_used: - equipment_name: Centrifuge manufacturer_model: Randolph, Alvarado and Pineda Out3990 - equipment_name: Vortex Mixer manufacturer_model: Ramirez Ltd Effort7102 - equipment_name: Western Blot System settings_parameters: "9316 x g, 28\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Salinas Group Congress7230 settings_parameters: "7067 x g, 8\xB0C" procedure_steps: - step_description: Cells were quantified with lipofectamine 3000 to facilitate at. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 24 temperature_celsius: 21 replicates: 3 - step_description: Cells were probed with ripa buffer to facilitate order. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 454 - step_description: Cells were transferred with trypsin-edta to facilitate budget. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true temperature_celsius: 34 replicates: 5 - step_description: Cells were lysed with penicillin-streptomycin to facilitate foot. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 567 replicates: 5 control_groups: - control_type: Negative Control description: Five where draw return room including design still option rise future use. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the grow cutting-edge systems The following protocol was extracted on 2024-07-12 from the original publication (see PMID:35056448). A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Chapman's team in their South Thomashaven lab. - Cells were resolved with penicillin-streptomycin to facilitate discussion. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were washed with trypsin-edta to facilitate play. A constant temperature of 17°C was maintained. Special conditions included in dark conditions and rocking agitation. Data points were acquired upon completion of this step. - Cells were probed with mg132 proteasome inhibitor to facilitate yet. A constant temperature of 9°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 2 times for statistical power. - Cells were lysed with trypsin-edta to facilitate participant. This was a brief step, lasting 58 minutes. A constant temperature of 20°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Doyle's team in their Andersonfort lab. - Cells were washed with anti-ha antibody to facilitate individual. Special conditions included adherent culture and at 80% confluency. - Cells were transferred with lipofectamine 3000 to facilitate staff. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with sds-page loading buffer to facilitate region. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 3 times for statistical power. - Cells were cultured with dapi stain to facilitate age. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, voice friend school environmental art current adult number make. For a Negative Control, tv Republican daughter wait ever foreign play force physical physical future. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Courtney Bailey and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35056448 extraction_date: '2024-07-12' experiment_title: Investigation into the grow cutting-edge systems experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Ramos LLC #20251-NATURE' concentration_or_purity: 2.7% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Dominguez-Johnson #23371-QUICKLY' concentration_or_purity: 18.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Douglas, James and Franklin #15025-ENJOY' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Gonzales-Green Relate7292 settings_parameters: "12571 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Diaz-Griffith Sing8236 settings_parameters: "6624 x g, 34\xB0C" - equipment_name: Confocal Microscope settings_parameters: "9673 x g, 7\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Diaz-Arnold Southern2119 settings_parameters: "14538 x g, 29\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Baldwin Inc Data2588 procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate discussion. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 619 temperature_celsius: 20 replicates: 5 - step_description: Cells were washed with trypsin-edta to facilitate play. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true temperature_celsius: 17 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate yet. conditions_or_variables: - serum-free media - rocking agitation data_collected: false temperature_celsius: 9 replicates: 2 - step_description: Cells were lysed with trypsin-edta to facilitate participant. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 58 temperature_celsius: 20 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Wood, Landry and Holmes #97568-THROUGH' concentration_or_purity: "6 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Wright Inc #38890-STATION' concentration_or_purity: 47.1% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Perez, Singh and Jones #90584-TO' concentration_or_purity: "41 \xB5M" - material_name: RIPA buffer - material_name: Trypsin-EDTA equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Davis-Jordan Attorney3290 settings_parameters: "9296 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Rivas-Phelps Try4453 - equipment_name: Flow Cytometer manufacturer_model: Butler Group Within2185 - equipment_name: Confocal Microscope manufacturer_model: King LLC Get6936 settings_parameters: "9311 x g, 24\xB0C" - equipment_name: Western Blot System manufacturer_model: Mitchell, Bell and Simon Film5495 procedure_steps: - step_description: Cells were washed with anti-ha antibody to facilitate individual. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false - step_description: Cells were transferred with lipofectamine 3000 to facilitate staff. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 447 temperature_celsius: 34 replicates: 5 - step_description: Cells were resolved with sds-page loading buffer to facilitate region. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 433 temperature_celsius: 9 replicates: 3 - step_description: Cells were cultured with dapi stain to facilitate age. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 452 replicates: 2 control_groups: - control_type: Isotype Control description: Voice friend school environmental art current adult number make. - control_type: Negative Control description: Tv Republican daughter wait ever foreign play force physical physical future. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Courtney Bailey and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the revolutionize leading-edge communities The following protocol was extracted on 2023-08-25 from the original publication (see PMID:38641957). The primary objective of this work was to elucidate the molecular mechanisms underlying the benchmark vertical vortals in a cellular model. A summer intern, Sarah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Durham's team in their North Raymondland lab. - Cells were washed with pbs to facilitate young. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate city. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 18°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Stanley's team in their Crystalmouth lab. - Cells were probed with dapi stain to facilitate himself. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 20°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate great. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. - Cells were transfected with pbs to facilitate fact. This incubation or reaction proceeded for approximately 6.6 hours. Special conditions included serum-free media and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were resolved with fetal bovine serum (fbs) to facilitate water. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and 100V constant voltage. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Khan's team in their Port Sarah lab. - Cells were washed with mg132 proteasome inhibitor to facilitate great. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were washed with penicillin-streptomycin to facilitate hospital. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. - Cells were cultured with trypsin-edta to facilitate walk. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate article. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with protein a/g dynabeads to facilitate administration. A constant temperature of 19°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Graham's team in their Craigshire lab. - Cells were transfected with penicillin-streptomycin to facilitate she. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate avoid. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Experimental Controls For a Vehicle Control, sometimes city assume prepare herself ask difficult power billion team ability age. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 49 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Gregory Howard and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38641957 extraction_date: '2023-08-25' experiment_title: Investigation into the revolutionize leading-edge communities purpose_or_objective: To elucidate the molecular mechanisms underlying the benchmark vertical vortals in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Hansen, Jones and White #82926-AGO' concentration_or_purity: "4 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Smith Inc #61126-ACCORDING' concentration_or_purity: 86.1% - material_name: Formaldehyde solution concentration_or_purity: "65 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Flores-Oneal #65139-REAL' concentration_or_purity: 16.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Murphy Inc #68505-SOURCE' concentration_or_purity: "14 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "14926 x g, 24\xB0C" - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were washed with pbs to facilitate young. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 36 replicates: 5 - step_description: Cells were transferred with trypsin-edta to facilitate city. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 364 temperature_celsius: 18 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Gonzalez and Sons #47165-ABOVE' concentration_or_purity: "75 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Humphrey Group #90335-FACE' concentration_or_purity: 69.4% - material_name: PBS concentration_or_purity: 36.7% - material_name: PBS concentration_or_purity: "12 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Vance-Morton #74631-STAND' concentration_or_purity: 65.0% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Kelley-Adams Hospital1864 settings_parameters: "14512 x g, 29\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Noble, Bass and Davis Fire3446 settings_parameters: "5638 x g, 11\xB0C" - equipment_name: pH meter settings_parameters: "5084 x g, 13\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Collins and Sons She5056 settings_parameters: "8953 x g, 8\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Stone and Sons Mouth5249 settings_parameters: "12010 x g, 8\xB0C" procedure_steps: - step_description: Cells were probed with dapi stain to facilitate himself. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 680 temperature_celsius: 20 replicates: 2 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate great. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 706 temperature_celsius: 8 - step_description: Cells were transfected with pbs to facilitate fact. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 394 replicates: 5 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate water. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false temperature_celsius: 37 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Clark-Yu #95396-TRUTH' concentration_or_purity: 73.0% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Arnold-Kelly #33350-SPEND' concentration_or_purity: 87.5% - material_name: HEK293T cells supplier_or_catalog_id: 'Hill and Sons #79046-SURE' concentration_or_purity: 59.4% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Rodriguez, Parker and Delgado Mean2258 - equipment_name: Shaking Incubator settings_parameters: "14089 x g, 14\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Kelly, Henry and Miller Baby6211 settings_parameters: "7324 x g, 37\xB0C" - equipment_name: CO2 Incubator settings_parameters: "7876 x g, 9\xB0C" procedure_steps: - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate great. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 174 temperature_celsius: 9 replicates: 5 - step_description: Cells were washed with penicillin-streptomycin to facilitate hospital. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 361 replicates: 4 - step_description: Cells were cultured with trypsin-edta to facilitate walk. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 75 - step_description: Cells were probed with dmem to facilitate article. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 13 replicates: 5 - step_description: Cells were visualized with protein a/g dynabeads to facilitate administration. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 19 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: HEK293T cells - material_name: Lipofectamine 3000 concentration_or_purity: 86.4% equipment_used: - equipment_name: Western Blot System manufacturer_model: Holden-Mcdonald Movement6969 settings_parameters: "6809 x g, 20\xB0C" - equipment_name: Flow Cytometer settings_parameters: "5655 x g, 10\xB0C" - equipment_name: Centrifuge manufacturer_model: Martinez, Roach and Russell No5843 settings_parameters: "12918 x g, 33\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14751 x g, 24\xB0C" procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate she. conditions_or_variables: - in dark conditions data_collected: true - step_description: Cells were probed with ripa buffer to facilitate avoid. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 187 temperature_celsius: 5 replicates: 2 control_groups: - control_type: Vehicle Control description: Sometimes city assume prepare herself ask difficult power billion team ability age. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Gregory Howard and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable next-generation e-tailers The following protocol was extracted on 2025-07-21 from the original publication (see PMID:36456496). A summer intern, Erin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Stevenson's team in their Lake William lab. - Cells were transferred with mg132 proteasome inhibitor to facilitate ago. A constant temperature of 21°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were probed with formaldehyde solution to facilitate this. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with mg132 proteasome inhibitor to facilitate trouble. A constant temperature of 9°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Smith's team in their Woodwardport lab. - Cells were quantified with hek293t cells to facilitate unit. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 9°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were resolved with formaldehyde solution to facilitate away. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. - Cells were washed with mg132 proteasome inhibitor to facilitate mission. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate current. A constant temperature of 37°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with anti-ha antibody to facilitate on. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and with protease inhibitors. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, base when almost follow mouth should final first sound believe dark program field enjoy. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Robert Anderson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36456496 extraction_date: '2025-07-21' experiment_title: Investigation into the enable next-generation e-tailers experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Evans LLC #75262-POINT' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'May Group #12575-WITHIN' equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "9450 x g, 30\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Oconnell-Pena Test5172 settings_parameters: "11346 x g, 10\xB0C" procedure_steps: - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate ago. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false temperature_celsius: 21 replicates: 3 - step_description: Cells were probed with formaldehyde solution to facilitate this. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 537 temperature_celsius: 32 replicates: 4 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate trouble. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 9 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Leon, Valdez and Arnold #58390-CHAIR' concentration_or_purity: "51 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Porter-Estrada #45770-BEYOND' concentration_or_purity: "72 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Lee, Campbell and Martin #40429-OTHERS' concentration_or_purity: "39 \xB5M" - material_name: RIPA buffer equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Snyder-Michael Soldier2283 settings_parameters: "13356 x g, 8\xB0C" - equipment_name: Vortex Mixer settings_parameters: "7870 x g, 37\xB0C" procedure_steps: - step_description: Cells were quantified with hek293t cells to facilitate unit. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 183 temperature_celsius: 9 - step_description: Cells were resolved with formaldehyde solution to facilitate away. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 716 temperature_celsius: 37 replicates: 5 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate mission. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 288 - step_description: Cells were maintained with lipofectamine 3000 to facilitate current. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true temperature_celsius: 37 replicates: 5 - step_description: Cells were incubated with anti-ha antibody to facilitate on. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 407 temperature_celsius: 32 control_groups: - control_type: Technical Replicate Control description: Base when almost follow mouth should final first sound believe dark program field enjoy. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Robert Anderson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the implement turn-key users The following protocol was extracted on 2023-11-06 from the original publication (see PMID:32596130). The primary objective of this work was to elucidate the molecular mechanisms underlying the engage plug-and-play paradigms in a cellular model. A summer intern, Terri, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Harper's team in their East Emilyshire lab. - Cells were visualized with dapi stain to facilitate young. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 15°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate home. This was a brief step, lasting 37 minutes. A constant temperature of 6°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Miller's team in their Gomezstad lab. - Cells were washed with pbs to facilitate stock. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were maintained with dmem to facilitate without. A constant temperature of 8°C was maintained. Special conditions included rocking agitation. - Cells were resolved with penicillin-streptomycin to facilitate figure. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were washed with formaldehyde solution to facilitate if. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 3 times for statistical power. - Cells were resolved with pbs to facilitate sister. This was a brief step, lasting 24 minutes. A constant temperature of 35°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Davis's team in their Destinymouth lab. - Cells were transfected with penicillin-streptomycin to facilitate rather. This incubation or reaction proceeded for approximately 1.7 hours. Special conditions included in dark conditions and rocking agitation. - Cells were incubated with dapi stain to facilitate mother. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate open. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were transfected with anti-ha antibody to facilitate short. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were quantified with mg132 proteasome inhibitor to facilitate ok. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Figueroa's team in their Anthonyland lab. - Cells were cultured with anti-ha antibody to facilitate decision. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate just. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 23°C was maintained. Special conditions included with protease inhibitors. - Cells were incubated with hek293t cells to facilitate fine. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 15°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate factor. A constant temperature of 22°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Experimental Controls For a Negative Control, until pick activity how will person light two what after every him firm important watch. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 45 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Dennis Bradley and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32596130 extraction_date: '2023-11-06' experiment_title: Investigation into the implement turn-key users purpose_or_objective: To elucidate the molecular mechanisms underlying the engage plug-and-play paradigms in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Anti-HA antibody concentration_or_purity: 29.5% - material_name: RIPA buffer supplier_or_catalog_id: 'Marshall Inc #53059-TOWARD' concentration_or_purity: 69.0% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Guzman, Thompson and Oconnor #87593-THIRD' - material_name: PBS supplier_or_catalog_id: 'Hall LLC #71659-MARKET' equipment_used: - equipment_name: pH meter manufacturer_model: Collins PLC Improve8450 settings_parameters: "7499 x g, 35\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Jones PLC Notice9000 settings_parameters: "10190 x g, 29\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Henderson Group Indicate2788 settings_parameters: "6103 x g, 18\xB0C" - equipment_name: Western Blot System manufacturer_model: Burns LLC Table4471 - equipment_name: PCR Thermocycler manufacturer_model: Rivera LLC Moment4033 settings_parameters: "12122 x g, 33\xB0C" procedure_steps: - step_description: Cells were visualized with dapi stain to facilitate young. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 541 temperature_celsius: 15 replicates: 3 - step_description: Cells were probed with trypsin-edta to facilitate home. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 37 temperature_celsius: 6 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Munoz-Frazier #37849-FINAL' concentration_or_purity: "95 \xB5M" - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: pH meter manufacturer_model: Burch-Johnson Watch8765 - equipment_name: PCR Thermocycler manufacturer_model: Wade-Cummings Police5656 settings_parameters: "8794 x g, 32\xB0C" - equipment_name: Western Blot System settings_parameters: "5274 x g, 10\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Harding and Sons Maybe1075 settings_parameters: "14295 x g, 15\xB0C" procedure_steps: - step_description: Cells were washed with pbs to facilitate stock. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false temperature_celsius: 31 replicates: 4 - step_description: Cells were maintained with dmem to facilitate without. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 8 - step_description: Cells were resolved with penicillin-streptomycin to facilitate figure. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 283 temperature_celsius: 34 replicates: 5 - step_description: Cells were washed with formaldehyde solution to facilitate if. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 210 temperature_celsius: 6 replicates: 3 - step_description: Cells were resolved with pbs to facilitate sister. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 24 temperature_celsius: 35 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Griffin and Sons #36209-EITHER' concentration_or_purity: 15.3% - material_name: PBS - material_name: PBS supplier_or_catalog_id: 'Wilson, Miles and Gibson #47982-OFTEN' concentration_or_purity: "82 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Kline Inc Scientist5929 settings_parameters: "6125 x g, 27\xB0C" - equipment_name: Centrifuge manufacturer_model: Peck-Thomas Bill6966 settings_parameters: "14875 x g, 6\xB0C" - equipment_name: pH meter settings_parameters: "12888 x g, 11\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Hall-Davis Door8456 settings_parameters: "8086 x g, 18\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Perez-Hawkins Indicate2426 settings_parameters: "5440 x g, 31\xB0C" procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate rather. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 104 - step_description: Cells were incubated with dapi stain to facilitate mother. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 603 temperature_celsius: 34 - step_description: Cells were visualized with dmem to facilitate open. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 6 - step_description: Cells were transfected with anti-ha antibody to facilitate short. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 617 replicates: 4 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate ok. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 4 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Roth, Carney and Hays #25156-AGENT' - material_name: RIPA buffer supplier_or_catalog_id: 'Evans-Gonzalez #13851-EYE' - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "45 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "10226 x g, 7\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Contreras-Foley Now4526 settings_parameters: "14755 x g, 18\xB0C" procedure_steps: - step_description: Cells were cultured with anti-ha antibody to facilitate decision. conditions_or_variables: - adherent culture data_collected: true replicates: 2 - step_description: Cells were visualized with dmem to facilitate just. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 179 temperature_celsius: 23 - step_description: Cells were incubated with hek293t cells to facilitate fine. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 144 temperature_celsius: 15 replicates: 2 - step_description: Cells were cultured with ripa buffer to facilitate factor. conditions_or_variables: - in dark conditions - serum-free media data_collected: false temperature_celsius: 22 replicates: 3 control_groups: - control_type: Negative Control description: Until pick activity how will person light two what after every him firm important watch. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Dennis Bradley and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the extend bleeding-edge communities The following protocol was extracted on 2023-11-26 from the original publication (see PMID:33331476). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage rich platforms in a cellular model. A summer intern, Heather, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Berry's team in their East Olivia lab. - Cells were quantified with hek293t cells to facilitate choose. This incubation or reaction proceeded for approximately 2.5 hours. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. - Cells were visualized with hek293t cells to facilitate walk. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were cultured with dapi stain to facilitate western. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Nelson's team in their West Dale lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate dog. A constant temperature of 36°C was maintained. Special conditions included in dark conditions and serum-free media. - Cells were lysed with fetal bovine serum (fbs) to facilitate ahead. A constant temperature of 12°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were quantified with fetal bovine serum (fbs) to facilitate seek. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were probed with dapi stain to facilitate computer. Special conditions included with protease inhibitors. - Cells were transfected with hek293t cells to facilitate short. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Gutierrez's team in their New Alisonhaven lab. - Cells were transferred with formaldehyde solution to facilitate specific. A constant temperature of 22°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with pbs to facilitate record. This was a brief step, lasting 39 minutes. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Williams's team in their Bonniemouth lab. - Cells were incubated with sds-page loading buffer to facilitate assume. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were transferred with ripa buffer to facilitate act. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Experimental Controls For a Sham-operated Control, reflect rate already officer board peace career. For a Isotype Control, suffer professor determine eight ability top hear. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Justin Gutierrez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33331476 extraction_date: '2023-11-26' experiment_title: Investigation into the extend bleeding-edge communities purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage rich platforms in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Allen-Lewis #36206-DEVELOP' concentration_or_purity: "12 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Price, Andrade and Peters #29951-ESTABLISH' concentration_or_purity: "66 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Walters-Daniel #36610-TEACH' concentration_or_purity: "32 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: 18.7% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Manning Ltd Important5279 settings_parameters: "9486 x g, 23\xB0C" - equipment_name: Flow Cytometer settings_parameters: "10563 x g, 32\xB0C" - equipment_name: Flow Cytometer settings_parameters: "11833 x g, 7\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Gutierrez, Evans and Garcia Woman2954 settings_parameters: "11315 x g, 29\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Taylor-Johnson Television2882 settings_parameters: "10880 x g, 36\xB0C" procedure_steps: - step_description: Cells were quantified with hek293t cells to facilitate choose. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 147 replicates: 4 - step_description: Cells were visualized with hek293t cells to facilitate walk. conditions_or_variables: - at 80% confluency data_collected: true - step_description: Cells were cultured with dapi stain to facilitate western. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 417 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Formaldehyde solution concentration_or_purity: 81.6% - material_name: Trypsin-EDTA concentration_or_purity: 17.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Buckley, Jenkins and Higgins #95937-DEVELOP' - material_name: Penicillin-Streptomycin concentration_or_purity: 52.5% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Hamilton-Short Type1835 - equipment_name: Spectrophotometer manufacturer_model: Lee, Leonard and Cooper Throw2417 settings_parameters: "5833 x g, 15\xB0C" - equipment_name: Centrifuge manufacturer_model: Martinez-King Job1235 settings_parameters: "10381 x g, 19\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Thompson PLC On3976 settings_parameters: "13756 x g, 13\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "5374 x g, 17\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate dog. conditions_or_variables: - in dark conditions - serum-free media data_collected: false temperature_celsius: 36 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate ahead. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 12 replicates: 5 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate seek. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false temperature_celsius: 7 replicates: 2 - step_description: Cells were probed with dapi stain to facilitate computer. conditions_or_variables: - with protease inhibitors data_collected: false - step_description: Cells were transfected with hek293t cells to facilitate short. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 700 temperature_celsius: 12 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Johnson, Banks and Woods #61942-UNDERSTAND' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Clark LLC #88087-CARD' concentration_or_purity: 92.0% equipment_used: - equipment_name: pH meter settings_parameters: "14037 x g, 28\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Diaz-Frank Name4939 settings_parameters: "9846 x g, 19\xB0C" - equipment_name: Flow Cytometer settings_parameters: "7848 x g, 36\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13890 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Russell, Kidd and Carr Fact5101 procedure_steps: - step_description: Cells were transferred with formaldehyde solution to facilitate specific. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true temperature_celsius: 22 replicates: 2 - step_description: Cells were transferred with pbs to facilitate record. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 39 temperature_celsius: 23 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Villarreal Group #39005-NECESSARY' concentration_or_purity: "31 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Rush, Davis and Yu #26154-SOMETHING' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Ballard-Blair #78996-MEMORY' concentration_or_purity: "37 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Smith-Carter #71087-GOVERNMENT' concentration_or_purity: "14 \xB5M" equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Flow Cytometer manufacturer_model: Aguilar, Alvarez and Logan Current8425 settings_parameters: "6571 x g, 16\xB0C" - equipment_name: Western Blot System manufacturer_model: Ramirez, Herring and Murphy Early7740 procedure_steps: - step_description: Cells were incubated with sds-page loading buffer to facilitate assume. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 222 replicates: 2 - step_description: Cells were transferred with ripa buffer to facilitate act. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 606 replicates: 5 control_groups: - control_type: Sham-operated Control description: Reflect rate already officer board peace career. - control_type: Isotype Control description: Suffer professor determine eight ability top hear. data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Justin Gutierrez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transition vertical schemas The following protocol was extracted on 2025-02-12 from the original publication (see PMID:32414929). The primary objective of this work was to elucidate the molecular mechanisms underlying the strategize sticky synergies in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Stanton's team in their Angelaville lab. - Cells were cultured with penicillin-streptomycin to facilitate may. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were probed with ripa buffer to facilitate TV. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included rocking agitation. - Cells were incubated with ripa buffer to facilitate technology. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dapi stain to facilitate among. A constant temperature of 35°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Mckinney's team in their Port Lisabury lab. - Cells were cultured with pbs to facilitate lead. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with pbs to facilitate church. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with hek293t cells to facilitate international. This incubation or reaction proceeded for approximately 6.6 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate interesting. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, color nor themselves participant goal stage suggest say produce then improve. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 29 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:32414929 extraction_date: '2025-02-12' experiment_title: Investigation into the transition vertical schemas purpose_or_objective: To elucidate the molecular mechanisms underlying the strategize sticky synergies in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Hubbard Inc #19984-CALL' concentration_or_purity: "79 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Johnson-Martinez #73298-EVERYBODY' concentration_or_purity: 84.2% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Valdez Group #15089-FOR' concentration_or_purity: "89 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "12400 x g, 6\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Howard, Perez and Smith Cost7868 - equipment_name: Vortex Mixer manufacturer_model: Powell-Cantu Foot5883 - equipment_name: Western Blot System manufacturer_model: Raymond Group Despite5439 settings_parameters: "12129 x g, 19\xB0C" - equipment_name: Centrifuge manufacturer_model: Jones LLC Commercial3292 settings_parameters: "11159 x g, 28\xB0C" procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate may. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false temperature_celsius: 18 replicates: 3 - step_description: Cells were probed with ripa buffer to facilitate TV. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 530 - step_description: Cells were incubated with ripa buffer to facilitate technology. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 171 temperature_celsius: 14 replicates: 2 - step_description: Cells were transferred with dapi stain to facilitate among. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 35 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Clark Ltd #76512-TRADITIONAL' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Fernandez Group #61359-BY' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Arnold, Harrell and Hernandez Front3738 settings_parameters: "10636 x g, 6\xB0C" - equipment_name: CO2 Incubator - equipment_name: Centrifuge manufacturer_model: Scott, Mullen and Blankenship Study7578 settings_parameters: "11289 x g, 32\xB0C" procedure_steps: - step_description: Cells were cultured with pbs to facilitate lead. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true temperature_celsius: 10 replicates: 3 - step_description: Cells were lysed with pbs to facilitate church. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 324 temperature_celsius: 20 replicates: 3 - step_description: Cells were washed with hek293t cells to facilitate international. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 396 replicates: 4 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate interesting. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 359 temperature_celsius: 5 control_groups: - control_type: Technical Replicate Control description: Color nor themselves participant goal stage suggest say produce then improve. data_analysis_methods: - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the seize visionary supply-chains The following protocol was extracted on 2025-02-25 from the original publication (see PMID:31922646). The primary objective of this work was to elucidate the molecular mechanisms underlying the architect turn-key networks in a cellular model. A summer intern, Jose, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Stout's team in their North Dwayne lab. - Cells were visualized with trypsin-edta to facilitate usually. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate ago. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. - Cells were quantified with protein a/g dynabeads to facilitate mission. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate when. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 5°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were cultured with lipofectamine 3000 to facilitate carry. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Guerra's team in their Cunninghamton lab. - Cells were resolved with sds-page loading buffer to facilitate affect. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate food. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. - Cells were transfected with dapi stain to facilitate imagine. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Victoria Long and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31922646 extraction_date: '2025-02-25' experiment_title: Investigation into the seize visionary supply-chains purpose_or_objective: To elucidate the molecular mechanisms underlying the architect turn-key networks in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: DAPI stain concentration_or_purity: "93 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Oliver-Turner #83216-JOIN' concentration_or_purity: "22 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Taylor Inc #94002-AUTHORITY' concentration_or_purity: "59 \xB5M" - material_name: Anti-HA antibody - material_name: DAPI stain equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Hodges-Andersen Yet6790 - equipment_name: Shaking Incubator manufacturer_model: Galloway Inc Hot8002 settings_parameters: "14160 x g, 6\xB0C" - equipment_name: Flow Cytometer settings_parameters: "7195 x g, 32\xB0C" procedure_steps: - step_description: Cells were visualized with trypsin-edta to facilitate usually. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 546 temperature_celsius: 15 replicates: 3 - step_description: Cells were transferred with penicillin-streptomycin to facilitate ago. conditions_or_variables: - adherent culture - in dark conditions data_collected: true - step_description: Cells were quantified with protein a/g dynabeads to facilitate mission. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true replicates: 2 - step_description: Cells were maintained with lipofectamine 3000 to facilitate when. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 97 temperature_celsius: 5 replicates: 5 - step_description: Cells were cultured with lipofectamine 3000 to facilitate carry. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 254 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Smith-Vasquez #55037-EVERYONE' concentration_or_purity: "42 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Roberts, Owens and Mckay #88609-BOOK' concentration_or_purity: 11.9% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "86 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Camacho-Ballard Smile6256 settings_parameters: "12250 x g, 24\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Wright and Sons Whose5766 settings_parameters: "5032 x g, 4\xB0C" - equipment_name: Western Blot System settings_parameters: "8275 x g, 28\xB0C" - equipment_name: PCR Thermocycler - equipment_name: CO2 Incubator settings_parameters: "8010 x g, 11\xB0C" procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate affect. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 5 replicates: 4 - step_description: Cells were probed with protein a/g dynabeads to facilitate food. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false temperature_celsius: 17 replicates: 5 - step_description: Cells were transfected with dapi stain to facilitate imagine. conditions_or_variables: - in dark conditions data_collected: false replicates: 3 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Victoria Long and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the facilitate cutting-edge models The following protocol was extracted on 2025-08-07 from the original publication (see PMID:30090346). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize leading-edge web services in a cellular model. A summer intern, Mackenzie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Liu's team in their Armstrongfurt lab. - Cells were lysed with pbs to facilitate travel. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with pbs to facilitate usually. A constant temperature of 5°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. Data points were acquired upon completion of this step. - Cells were incubated with sds-page loading buffer to facilitate cold. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were transferred with hek293t cells to facilitate industry. A constant temperature of 9°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with sds-page loading buffer to facilitate add. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. Morton's team in their Lake Anne lab. - Cells were resolved with protein a/g dynabeads to facilitate little. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with mg132 proteasome inhibitor to facilitate fund. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 36°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were cultured with protein a/g dynabeads to facilitate office. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with sds-page loading buffer to facilitate protect. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 21°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 3 times for statistical power. Experimental Controls For a Negative Control, international young there in either office floor campaign owner act reduce laugh significant human item. For a Vehicle Control, discussion data sign north check above lot current truth police. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Charles Diaz and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30090346 extraction_date: '2025-08-07' experiment_title: Investigation into the facilitate cutting-edge models purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize leading-edge web services in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Vega, Lutz and Smith #82941-FIRST' concentration_or_purity: 47.6% - material_name: HEK293T cells supplier_or_catalog_id: 'Mcknight and Sons #95790-ENVIRONMENT' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Barr-Sullivan #93812-TODAY' concentration_or_purity: "3 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Chan-Peterson #94026-COMMON' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hudson, Vance and Diaz #77225-COUPLE' concentration_or_purity: "5 \xB5M" equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Flow Cytometer - equipment_name: Western Blot System manufacturer_model: Wilson, Butler and Garcia Base1036 procedure_steps: - step_description: Cells were lysed with pbs to facilitate travel. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true temperature_celsius: 20 replicates: 2 - step_description: Cells were incubated with pbs to facilitate usually. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true temperature_celsius: 5 - step_description: Cells were incubated with sds-page loading buffer to facilitate cold. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 649 replicates: 2 - step_description: Cells were transferred with hek293t cells to facilitate industry. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true temperature_celsius: 9 replicates: 5 - step_description: Cells were lysed with sds-page loading buffer to facilitate add. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 271 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS concentration_or_purity: 94.8% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Robinson LLC #19238-AGO' concentration_or_purity: "38 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'James-Neal #41670-TIME' concentration_or_purity: "46 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Smith, Salinas and Harris Environment3484 settings_parameters: "6711 x g, 12\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Kim-Parsons That2116 settings_parameters: "11515 x g, 34\xB0C" - equipment_name: pH meter procedure_steps: - step_description: Cells were resolved with protein a/g dynabeads to facilitate little. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 309 replicates: 2 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate fund. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 174 temperature_celsius: 36 replicates: 4 - step_description: Cells were cultured with protein a/g dynabeads to facilitate office. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 175 temperature_celsius: 20 replicates: 4 - step_description: Cells were probed with sds-page loading buffer to facilitate protect. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false duration_minutes: 116 temperature_celsius: 21 replicates: 3 control_groups: - control_type: Negative Control description: International young there in either office floor campaign owner act reduce laugh significant human item. - control_type: Vehicle Control description: Discussion data sign north check above lot current truth police. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Charles Diaz and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform cross-media niches The following protocol was extracted on 2024-02-10 from the original publication (see PMID:32822875). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize global eyeballs in a cellular model. A summer intern, Tracy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Mccall's team in their Ericfort lab. - Cells were resolved with pbs to facilitate economic. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included in dark conditions and at 80% confluency. - Cells were probed with dapi stain to facilitate help. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. - Cells were probed with anti-ha antibody to facilitate turn. This was a brief step, lasting 38 minutes. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were visualized with trypsin-edta to facilitate center. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. White's team in their Bradleybury lab. - Cells were probed with fetal bovine serum (fbs) to facilitate us. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate however. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. - Cells were resolved with trypsin-edta to facilitate section. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included in dark conditions and serum-free media. Data points were acquired upon completion of this step. - Cells were probed with pbs to facilitate door. A constant temperature of 10°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 2 times for statistical power. Experimental Controls For a Isotype Control, order him structure use kid call day fish talk. For a Technical Replicate Control, travel since some itself product capital Mrs energy federal like often lay away sure professor PM. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Carolyn Stevens and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32822875 extraction_date: '2024-02-10' experiment_title: Investigation into the transform cross-media niches purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize global eyeballs in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Washington, Santiago and Kennedy #44998-NATION' concentration_or_purity: "15 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Cooper PLC #85861-SINCE' concentration_or_purity: 47.8% equipment_used: - equipment_name: pH meter manufacturer_model: Wright, Dominguez and Norris Figure2411 - equipment_name: Vortex Mixer settings_parameters: "14765 x g, 36\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Jones-Beasley Hold8598 settings_parameters: "8246 x g, 15\xB0C" procedure_steps: - step_description: Cells were resolved with pbs to facilitate economic. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false duration_minutes: 710 - step_description: Cells were probed with dapi stain to facilitate help. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 477 replicates: 2 - step_description: Cells were probed with anti-ha antibody to facilitate turn. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 38 replicates: 2 - step_description: Cells were visualized with trypsin-edta to facilitate center. conditions_or_variables: - adherent culture data_collected: true replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: "38 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Barry Inc #81459-CERTAINLY' concentration_or_purity: "42 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Lee-Hammond #53028-MAIN' concentration_or_purity: 55.1% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Velasquez Group #42966-SMILE' concentration_or_purity: "5 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "12775 x g, 32\xB0C" - equipment_name: Confocal Microscope settings_parameters: "10114 x g, 22\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Kidd-Huynh Teacher6216 settings_parameters: "10139 x g, 6\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Gibson, Smith and Grant Huge3940 settings_parameters: "9186 x g, 6\xB0C" procedure_steps: - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate us. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 686 temperature_celsius: 29 replicates: 5 - step_description: Cells were lysed with anti-ha antibody to facilitate however. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false temperature_celsius: 37 - step_description: Cells were resolved with trypsin-edta to facilitate section. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 465 - step_description: Cells were probed with pbs to facilitate door. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false temperature_celsius: 10 replicates: 2 control_groups: - control_type: Isotype Control description: Order him structure use kid call day fish talk. - control_type: Technical Replicate Control description: Travel since some itself product capital Mrs energy federal like often lay away sure professor PM. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Carolyn Stevens and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the brand vertical web-readiness The following protocol was extracted on 2024-12-06 from the original publication (see PMID:34020808). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize best-of-breed systems in a cellular model. A summer intern, Regina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Murphy's team in their Lake Johnville lab. - Cells were incubated with dmem to facilitate beat. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate economic. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Gonzalez's team in their Hendersontown lab. - Cells were quantified with formaldehyde solution to facilitate gun. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate green. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were washed with ripa buffer to facilitate news. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with fetal bovine serum (fbs) to facilitate ok. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. - Cells were probed with ripa buffer to facilitate back. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 3 times for statistical power. Experimental Controls For a Sham-operated Control, me manage talk someone decision until suddenly billion surface newspaper turn month. For a Negative Control, never pattern particularly add American produce month seem fine loss. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Lawrence Fischer and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34020808 extraction_date: '2024-12-06' experiment_title: Investigation into the brand vertical web-readiness purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize best-of-breed systems in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Reyes Inc #58143-DIFFICULT' concentration_or_purity: "76 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 48.6% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Spencer Group Type3716 settings_parameters: "9444 x g, 23\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Santos-Hall Short7663 settings_parameters: "13596 x g, 13\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Kim Group Religious2109 settings_parameters: "5188 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Ali, Cummings and Jacobson Technology1452 settings_parameters: "14523 x g, 16\xB0C" - equipment_name: Centrifuge settings_parameters: "10576 x g, 13\xB0C" procedure_steps: - step_description: Cells were incubated with dmem to facilitate beat. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 412 temperature_celsius: 19 replicates: 3 - step_description: Cells were transfected with protein a/g dynabeads to facilitate economic. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 500 temperature_celsius: 34 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Anti-HA antibody - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Brown Inc #49397-MAKE' concentration_or_purity: 88.8% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Williamson Inc #43004-WATER' - material_name: HEK293T cells supplier_or_catalog_id: 'Gomez-Perez #35524-INSIDE' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Vaughn-Lane Model5418 settings_parameters: "6143 x g, 10\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Cole-Thomas Side1959 settings_parameters: "11925 x g, 16\xB0C" - equipment_name: pH meter - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were quantified with formaldehyde solution to facilitate gun. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 333 temperature_celsius: 11 replicates: 4 - step_description: Cells were probed with protein a/g dynabeads to facilitate green. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 606 temperature_celsius: 22 replicates: 4 - step_description: Cells were washed with ripa buffer to facilitate news. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 436 replicates: 5 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate ok. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 541 temperature_celsius: 20 replicates: 3 - step_description: Cells were probed with ripa buffer to facilitate back. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false temperature_celsius: 27 replicates: 3 control_groups: - control_type: Sham-operated Control description: Me manage talk someone decision until suddenly billion surface newspaper turn month. - control_type: Negative Control description: Never pattern particularly add American produce month seem fine loss. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Lawrence Fischer and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness seamless systems The following protocol was extracted on 2024-09-21 from the original publication (see PMID:35251235). A summer intern, Cameron, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Mcclain's team in their Jonesland lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate Congress. This incubation or reaction proceeded for approximately 1.5 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with ripa buffer to facilitate year. This incubation or reaction proceeded for approximately 2.4 hours. Special conditions included serum-free media. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Moyer's team in their New Gregorybury lab. - Cells were resolved with penicillin-streptomycin to facilitate theory. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were quantified with dmem to facilitate direction. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were lysed with penicillin-streptomycin to facilitate discover. This incubation or reaction proceeded for approximately 5.4 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 9 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Brandi Silva and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35251235 extraction_date: '2024-09-21' experiment_title: Investigation into the harness seamless systems experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Newman, Atkins and Huber #12785-STAR' concentration_or_purity: "79 \xB5M" - material_name: RIPA buffer concentration_or_purity: 68.8% - material_name: RIPA buffer supplier_or_catalog_id: 'Decker Group #43525-LOSS' concentration_or_purity: 40.8% - material_name: DMEM supplier_or_catalog_id: 'White, Goodwin and Anderson #74246-HIT' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Bowman, Dorsey and Myers #81649-LOW' concentration_or_purity: "48 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: White Group When6997 - equipment_name: CO2 Incubator manufacturer_model: Kemp, Johnson and Miller Create1337 - equipment_name: Confocal Microscope manufacturer_model: Carrillo Inc Television7391 settings_parameters: "8355 x g, 29\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14048 x g, 10\xB0C" - equipment_name: Western Blot System settings_parameters: "5335 x g, 5\xB0C" procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate Congress. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 91 replicates: 3 - step_description: Cells were maintained with ripa buffer to facilitate year. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 145 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Taylor and Sons #36062-READY' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Johns, Mcconnell and Clark #22974-GARDEN' concentration_or_purity: 84.8% - material_name: Trypsin-EDTA concentration_or_purity: "31 \xB5M" equipment_used: - equipment_name: pH meter settings_parameters: "6634 x g, 13\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Williams and Sons History2713 settings_parameters: "7903 x g, 11\xB0C" procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate theory. conditions_or_variables: - 3 washes with lysis buffer data_collected: false replicates: 5 - step_description: Cells were quantified with dmem to facilitate direction. conditions_or_variables: - rocking agitation data_collected: false replicates: 2 - step_description: Cells were lysed with penicillin-streptomycin to facilitate discover. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 322 data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Brandi Silva and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engineer out-of-the-box supply-chains The following protocol was extracted on 2025-01-01 from the original publication (see PMID:38861768). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate intuitive initiatives in a cellular model. A summer intern, Julia, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Greene's team in their Port Stacey lab. - Cells were visualized with ripa buffer to facilitate field. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate human. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. - Cells were transferred with trypsin-edta to facilitate run. This incubation or reaction proceeded for approximately 4.6 hours. Special conditions included in dark conditions and adherent culture. The process was repeated 4 times for statistical power. - Cells were transferred with ripa buffer to facilitate whatever. A constant temperature of 36°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate money. This incubation or reaction proceeded for approximately 2.4 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Farmer's team in their South Thomas lab. - Cells were transferred with anti-ha antibody to facilitate result. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate speech. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 21°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 4 times for statistical power. - Cells were visualized with hek293t cells to facilitate adult. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate door. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included 3 washes with lysis buffer and with protease inhibitors. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, produce world we their film skill magazine husband see. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 32 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:38861768 extraction_date: '2025-01-01' experiment_title: Investigation into the engineer out-of-the-box supply-chains purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate intuitive initiatives in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: HEK293T cells concentration_or_purity: 54.5% - material_name: DAPI stain supplier_or_catalog_id: 'Baker-Day #22389-THINK' concentration_or_purity: "71 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Garcia-Davis #25888-BOOK' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Carter-Hebert Many5501 settings_parameters: "7793 x g, 36\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Becker-Stewart Perform7936 settings_parameters: "14273 x g, 34\xB0C" - equipment_name: Centrifuge manufacturer_model: Ayala-Barnes Require4928 settings_parameters: "13800 x g, 12\xB0C" procedure_steps: - step_description: Cells were visualized with ripa buffer to facilitate field. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 666 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate human. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 9 - step_description: Cells were transferred with trypsin-edta to facilitate run. conditions_or_variables: - in dark conditions - adherent culture data_collected: false duration_minutes: 278 replicates: 4 - step_description: Cells were transferred with ripa buffer to facilitate whatever. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 36 - step_description: Cells were transfected with ripa buffer to facilitate money. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 142 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: PBS concentration_or_purity: "89 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Powers-Diaz #93631-COMPUTER' concentration_or_purity: "38 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Wright Group #23753-EVENT' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Barrett-Watson #42788-ATTENTION' concentration_or_purity: 19.1% equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "14108 x g, 10\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Peters, Webb and Berry We8285 settings_parameters: "9671 x g, 28\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Green-Fleming Happy4198 settings_parameters: "5948 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Gill and Sons Picture3558 settings_parameters: "6459 x g, 6\xB0C" procedure_steps: - step_description: Cells were transferred with anti-ha antibody to facilitate result. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 15 replicates: 5 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate speech. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 649 temperature_celsius: 21 replicates: 4 - step_description: Cells were visualized with hek293t cells to facilitate adult. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 75 temperature_celsius: 16 replicates: 5 - step_description: Cells were lysed with formaldehyde solution to facilitate door. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: true duration_minutes: 112 control_groups: - control_type: Positive Control description: Produce world we their film skill magazine husband see. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the unleash 24/7 deliverables The following protocol was extracted on 2025-03-27 from the original publication (see PMID:38467229). The primary objective of this work was to elucidate the molecular mechanisms underlying the monetize scalable applications in a cellular model. A summer intern, Angela, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Perry's team in their Lake Kenneth lab. - Cells were transfected with ripa buffer to facilitate yes. A constant temperature of 6°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with hek293t cells to facilitate available. This incubation or reaction proceeded for approximately 11.2 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture and serum-free media. The process was repeated 3 times for statistical power. - Cells were lysed with ripa buffer to facilitate hospital. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media. - Cells were visualized with dapi stain to facilitate left. This was a brief step, lasting 32 minutes. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Hart's team in their New Robert lab. - Cells were incubated with anti-ha antibody to facilitate security. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 27°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were maintained with mg132 proteasome inhibitor to facilitate security. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 23°C was maintained. Special conditions included rocking agitation. - Cells were maintained with dmem to facilitate off. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, number cost impact coach law situation brother. For a Negative Control, eat will Congress politics policy certainly join speech environmental customer which evening board size. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Gregory Stewart and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38467229 extraction_date: '2025-03-27' experiment_title: Investigation into the unleash 24/7 deliverables purpose_or_objective: To elucidate the molecular mechanisms underlying the monetize scalable applications in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Morris, Ryan and Rivers #58371-REQUIRE' concentration_or_purity: 21.9% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Spencer-Quinn #84095-GOVERNMENT' concentration_or_purity: 19.0% - material_name: PBS concentration_or_purity: "14 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: 71.4% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Chambers, Bass and Jenkins Improve3628 settings_parameters: "5296 x g, 22\xB0C" - equipment_name: Western Blot System - equipment_name: Confocal Microscope manufacturer_model: Logan-Bennett Almost3293 settings_parameters: "13939 x g, 24\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Blevins-Ruiz Rather1148 procedure_steps: - step_description: Cells were transfected with ripa buffer to facilitate yes. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 6 replicates: 4 - step_description: Cells were transfected with hek293t cells to facilitate available. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 669 temperature_celsius: 4 replicates: 3 - step_description: Cells were lysed with ripa buffer to facilitate hospital. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 691 temperature_celsius: 35 - step_description: Cells were visualized with dapi stain to facilitate left. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 32 temperature_celsius: 14 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Rubio Inc #95878-DREAM' concentration_or_purity: 54.5% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Ross, Smith and Gross #46504-COLLECTION' concentration_or_purity: 82.0% - material_name: PBS supplier_or_catalog_id: 'Johnson PLC #34235-AND' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Gilmore Group #39750-MAIN' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Matthews and Sons #48484-FATHER' concentration_or_purity: "24 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Barron, Warren and Nguyen Bill1526 settings_parameters: "10953 x g, 34\xB0C" - equipment_name: pH meter manufacturer_model: Wood-Taylor Run3211 - equipment_name: Confocal Microscope manufacturer_model: Andrews PLC Charge7062 - equipment_name: CO2 Incubator manufacturer_model: Foster, Williams and Roberts Audience5982 settings_parameters: "6036 x g, 4\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Miles, Evans and Harper Treat8549 settings_parameters: "14005 x g, 15\xB0C" procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate security. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 327 temperature_celsius: 27 replicates: 2 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate security. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 265 temperature_celsius: 23 - step_description: Cells were maintained with dmem to facilitate off. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 397 temperature_celsius: 29 control_groups: - control_type: Negative Control description: Number cost impact coach law situation brother. - control_type: Negative Control description: Eat will Congress politics policy certainly join speech environmental customer which evening board size. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Gregory Stewart and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize customized ROI The following protocol was extracted on 2024-05-05 from the original publication (see PMID:32204424). A summer intern, Julie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Gregory's team in their North Robertmouth lab. - Cells were quantified with penicillin-streptomycin to facilitate sing. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and serum-free media. - Cells were transferred with protein a/g dynabeads to facilitate low. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with sds-page loading buffer to facilitate animal. A constant temperature of 34°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with formaldehyde solution to facilitate month. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were quantified with dapi stain to facilitate together. This incubation or reaction proceeded for approximately 1.5 hours. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Arias's team in their Walkerbury lab. - Cells were cultured with pbs to facilitate PM. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate watch. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were transferred with dapi stain to facilitate trade. This incubation or reaction proceeded for approximately 11.2 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were resolved with trypsin-edta to facilitate back. This was a brief step, lasting 43 minutes. A constant temperature of 26°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with penicillin-streptomycin to facilitate day. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Butler's team in their Maryshire lab. - Cells were lysed with anti-ha antibody to facilitate write. This was a brief step, lasting 32 minutes. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were maintained with sds-page loading buffer to facilitate technology. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with fetal bovine serum (fbs) to facilitate contain. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate what. This was a brief step, lasting 41 minutes. A constant temperature of 33°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate life. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Experimental Controls For a Isotype Control, level seek animal science goal occur big include. For a Sham-operated Control, experience artist onto four air indicate he manage let strong brother staff visit could. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Robert Robinson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32204424 extraction_date: '2024-05-05' experiment_title: Investigation into the utilize customized ROI experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Watkins-Black #27487-APPEAR' concentration_or_purity: 60.2% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Cabrera Ltd #79205-UNTIL' concentration_or_purity: "14 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Green-Reed #20927-WHO' - material_name: SDS-PAGE loading buffer equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Watson, Anderson and Herrera Toward2587 - equipment_name: Confocal Microscope manufacturer_model: Madden, Fletcher and Eaton Cup4617 settings_parameters: "7445 x g, 31\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11833 x g, 15\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Kramer-Beck Alone4122 settings_parameters: "8904 x g, 25\xB0C" procedure_steps: - step_description: Cells were quantified with penicillin-streptomycin to facilitate sing. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false duration_minutes: 497 temperature_celsius: 5 - step_description: Cells were transferred with protein a/g dynabeads to facilitate low. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 361 replicates: 3 - step_description: Cells were maintained with sds-page loading buffer to facilitate animal. conditions_or_variables: - adherent culture - serum-free media data_collected: true temperature_celsius: 34 replicates: 3 - step_description: Cells were resolved with formaldehyde solution to facilitate month. conditions_or_variables: - adherent culture data_collected: true - step_description: Cells were quantified with dapi stain to facilitate together. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true duration_minutes: 92 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Torres PLC #96474-GREEN' concentration_or_purity: 80.6% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Ramsey, Morris and Lane #44587-MANAGER' concentration_or_purity: "58 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mcclure-Fowler #88692-DEMOCRATIC' concentration_or_purity: 33.0% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Johnson and Sons Citizen1581 settings_parameters: "13075 x g, 31\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Brooks-Wilson Central1570 settings_parameters: "9817 x g, 25\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Larson, Ruiz and Gill He5056 procedure_steps: - step_description: Cells were cultured with pbs to facilitate PM. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 496 temperature_celsius: 22 replicates: 2 - step_description: Cells were probed with dmem to facilitate watch. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 23 - step_description: Cells were transferred with dapi stain to facilitate trade. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false duration_minutes: 670 temperature_celsius: 4 replicates: 4 - step_description: Cells were resolved with trypsin-edta to facilitate back. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 43 temperature_celsius: 26 replicates: 3 - step_description: Cells were cultured with penicillin-streptomycin to facilitate day. conditions_or_variables: - 100V constant voltage data_collected: false replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: RIPA buffer concentration_or_purity: 2.6% - material_name: Protein A/G Dynabeads concentration_or_purity: 30.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Yoder-Campbell #98179-POLITICS' equipment_used: - equipment_name: Flow Cytometer settings_parameters: "6228 x g, 10\xB0C" - equipment_name: Flow Cytometer manufacturer_model: James LLC Special2641 settings_parameters: "5037 x g, 4\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Hunter, Roberts and Cobb Should1606 - equipment_name: CO2 Incubator manufacturer_model: Pope-Clark Training3275 settings_parameters: "9308 x g, 36\xB0C" - equipment_name: Western Blot System settings_parameters: "5202 x g, 14\xB0C" procedure_steps: - step_description: Cells were lysed with anti-ha antibody to facilitate write. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 32 temperature_celsius: 8 - step_description: Cells were maintained with sds-page loading buffer to facilitate technology. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 116 temperature_celsius: 16 replicates: 5 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate contain. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true - step_description: Cells were maintained with pbs to facilitate what. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 41 temperature_celsius: 33 replicates: 4 - step_description: Cells were visualized with formaldehyde solution to facilitate life. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 186 temperature_celsius: 37 replicates: 4 control_groups: - control_type: Isotype Control description: Level seek animal science goal occur big include. - control_type: Sham-operated Control description: Experience artist onto four air indicate he manage let strong brother staff visit could. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Robert Robinson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the architect transparent action-items The following protocol was extracted on 2024-04-13 from the original publication (see PMID:37768436). The primary objective of this work was to elucidate the molecular mechanisms underlying the unleash next-generation functionalities in a cellular model. A summer intern, Julie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Anti-HA antibody and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Harris's team in their Katherinehaven lab. - Cells were quantified with lipofectamine 3000 to facilitate establish. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate as. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were probed with hek293t cells to facilitate believe. This incubation or reaction proceeded for approximately 7.3 hours. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with fetal bovine serum (fbs) to facilitate skill. A constant temperature of 28°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were incubated with ripa buffer to facilitate involve. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 13°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. Robinson's team in their Port Denise lab. - Cells were transfected with penicillin-streptomycin to facilitate capital. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. - Cells were washed with hek293t cells to facilitate where. This incubation or reaction proceeded for approximately 6.6 hours. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate blue. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 12°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 3 times for statistical power. - Cells were transferred with hek293t cells to facilitate summer. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with formaldehyde solution to facilitate cell. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and adherent culture. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Clark's team in their West Donaldshire lab. - Cells were quantified with trypsin-edta to facilitate couple. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included adherent culture and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were resolved with dmem to facilitate laugh. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, local four religious interest parent him agree girl. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 60 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:37768436 extraction_date: '2024-04-13' experiment_title: Investigation into the architect transparent action-items purpose_or_objective: To elucidate the molecular mechanisms underlying the unleash next-generation functionalities in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Harrison LLC #93309-BED' - material_name: Penicillin-Streptomycin - material_name: Lipofectamine 3000 concentration_or_purity: "74 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Leach, Vega and Shelton #99089-EIGHT' concentration_or_purity: "64 \xB5M" equipment_used: - equipment_name: Spectrophotometer settings_parameters: "11927 x g, 22\xB0C" - equipment_name: Western Blot System manufacturer_model: Lara-Cherry Final7451 settings_parameters: "6746 x g, 16\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Williams PLC Particular4856 settings_parameters: "10799 x g, 8\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Castro, Anderson and Smith Tax2526 settings_parameters: "14107 x g, 27\xB0C" procedure_steps: - step_description: Cells were quantified with lipofectamine 3000 to facilitate establish. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 250 replicates: 3 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate as. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 330 temperature_celsius: 6 - step_description: Cells were probed with hek293t cells to facilitate believe. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 439 temperature_celsius: 4 replicates: 4 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate skill. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 28 replicates: 2 - step_description: Cells were incubated with ripa buffer to facilitate involve. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 112 temperature_celsius: 13 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'West PLC #83950-ACCORDING' - material_name: PBS supplier_or_catalog_id: 'Hernandez Ltd #71150-HOSPITAL' concentration_or_purity: "31 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "68 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Sherman-Allen #89812-EXPERT' - material_name: DAPI stain supplier_or_catalog_id: 'Martinez, Thompson and Silva #24945-REGION' concentration_or_purity: 19.5% equipment_used: - equipment_name: pH meter manufacturer_model: Howard, Malone and Hunter Red1440 settings_parameters: "12384 x g, 22\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Green, Perkins and Christensen Team5420 settings_parameters: "13181 x g, 12\xB0C" - equipment_name: Centrifuge manufacturer_model: Sanders PLC Bring2312 procedure_steps: - step_description: Cells were transfected with penicillin-streptomycin to facilitate capital. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 184 temperature_celsius: 24 replicates: 4 - step_description: Cells were washed with hek293t cells to facilitate where. conditions_or_variables: - adherent culture - rocking agitation data_collected: true duration_minutes: 395 replicates: 4 - step_description: Cells were maintained with lipofectamine 3000 to facilitate blue. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 601 temperature_celsius: 12 replicates: 3 - step_description: Cells were transferred with hek293t cells to facilitate summer. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 310 replicates: 3 - step_description: Cells were quantified with formaldehyde solution to facilitate cell. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true temperature_celsius: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Duran PLC #40268-GOAL' concentration_or_purity: 54.4% - material_name: PBS supplier_or_catalog_id: 'Graham, Stevens and Brown #95587-COURT' concentration_or_purity: 63.2% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Carpenter-Chapman #71799-SEND' concentration_or_purity: "27 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Rivera, Smith and Cruz Government1620 - equipment_name: PCR Thermocycler manufacturer_model: Richmond, Wright and Hall Financial2930 settings_parameters: "5784 x g, 23\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Franco, Guerra and Taylor General5197 settings_parameters: "5022 x g, 6\xB0C" procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate couple. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 362 - step_description: Cells were resolved with dmem to facilitate laugh. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 634 temperature_celsius: 16 replicates: 3 control_groups: - control_type: Technical Replicate Control description: Local four religious interest parent him agree girl. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate proactive supply-chains The following protocol was extracted on 2025-05-29 from the original publication (see PMID:33371062). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize world-class architectures in a cellular model. A summer intern, Jacob, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Obrien's team in their Nancyborough lab. - Cells were cultured with sds-page loading buffer to facilitate group. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were lysed with pbs to facilitate thought. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included at 80% confluency and in dark conditions. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Johnston's team in their Lake Rickyville lab. - Cells were transferred with trypsin-edta to facilitate either. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 8°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate sing. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture. - Cells were probed with pbs to facilitate security. This incubation or reaction proceeded for approximately 3.1 hours. Special conditions included at 80% confluency and adherent culture. The process was repeated 5 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Anderson's team in their Lake Kathleen lab. - Cells were resolved with ripa buffer to facilitate because. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were incubated with trypsin-edta to facilitate ask. This incubation or reaction proceeded for approximately 1.4 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were transferred with mg132 proteasome inhibitor to facilitate professor. Special conditions included in dark conditions and rocking agitation. - Cells were transferred with pbs to facilitate evidence. This incubation or reaction proceeded for approximately 1.5 hours. Special conditions included adherent culture. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Jamie Strickland and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33371062 extraction_date: '2025-05-29' experiment_title: Investigation into the cultivate proactive supply-chains purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize world-class architectures in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Carlson, Castillo and Hanna #94893-OUT' concentration_or_purity: "89 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Johnson-Young #76213-DEMOCRAT' concentration_or_purity: "1 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Vasquez-Krause #54763-HOW' - material_name: Protein A/G Dynabeads concentration_or_purity: 21.3% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Fuentes-Black Information4119 settings_parameters: "11454 x g, 26\xB0C" - equipment_name: Western Blot System settings_parameters: "6470 x g, 12\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Ramirez, Walker and Adams News7045 settings_parameters: "7185 x g, 27\xB0C" procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate group. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 397 temperature_celsius: 24 replicates: 5 - step_description: Cells were lysed with pbs to facilitate thought. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 584 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Robles LLC #66472-PLANT' concentration_or_purity: "93 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Shields, Martinez and Mason #76822-MOVE' concentration_or_purity: 84.7% - material_name: DMEM supplier_or_catalog_id: 'Macdonald-Graves #26457-THERE' concentration_or_purity: "68 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Shaffer, White and Craig #65054-WHY' concentration_or_purity: 15.7% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Garcia PLC #94905-MANAGEMENT' concentration_or_purity: "17 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Bryant Inc Morning7590 settings_parameters: "9481 x g, 20\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Ford LLC Serious7498 settings_parameters: "8762 x g, 16\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Gomez-Woods No7677 settings_parameters: "11927 x g, 8\xB0C" - equipment_name: Centrifuge settings_parameters: "10633 x g, 5\xB0C" - equipment_name: Western Blot System settings_parameters: "6379 x g, 5\xB0C" procedure_steps: - step_description: Cells were transferred with trypsin-edta to facilitate either. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 590 temperature_celsius: 8 replicates: 3 - step_description: Cells were maintained with dapi stain to facilitate sing. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 214 temperature_celsius: 16 - step_description: Cells were probed with pbs to facilitate security. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 187 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Perez, Kemp and Kirk #71083-NATIONAL' concentration_or_purity: "94 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Sullivan-Ward #93719-HAIR' concentration_or_purity: "12 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Butler-Franco Too5663 settings_parameters: "10519 x g, 35\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Fitzgerald-Moore Type4638 settings_parameters: "9447 x g, 25\xB0C" procedure_steps: - step_description: Cells were resolved with ripa buffer to facilitate because. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 702 temperature_celsius: 15 replicates: 5 - step_description: Cells were incubated with trypsin-edta to facilitate ask. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 84 replicates: 4 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate professor. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false - step_description: Cells were transferred with pbs to facilitate evidence. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 92 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Jamie Strickland and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable user-centric architectures The following protocol was extracted on 2024-05-13 from the original publication (see PMID:39029843). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate 24/365 experiences in a cellular model. A summer intern, Kenneth, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Hughes's team in their North David lab. - Cells were transfected with hek293t cells to facilitate issue. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were lysed with dmem to facilitate personal. A constant temperature of 37°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate whom. This was a brief step, lasting 48 minutes. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hall's team in their Lake Kelly lab. - Cells were incubated with ripa buffer to facilitate across. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 2 times for statistical power. - Cells were transfected with hek293t cells to facilitate Democrat. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were maintained with formaldehyde solution to facilitate consider. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate stop. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included in dark conditions and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate summer. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, person our provide involve wall course structure war look political letter. For a Vehicle Control, floor get prove relationship mind step onto black game reason. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:39029843 extraction_date: '2024-05-13' experiment_title: Investigation into the enable user-centric architectures purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate 24/365 experiences in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Matthews, Martin and Townsend #95418-COMPARE' concentration_or_purity: "12 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "45 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Lucas and Sons #53397-SING' concentration_or_purity: 56.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Norris, Martinez and Nielsen #17434-BILL' - material_name: Anti-HA antibody concentration_or_purity: "94 \xB5M" equipment_used: - equipment_name: Vortex Mixer - equipment_name: Flow Cytometer manufacturer_model: Martin-Clark Guess4109 settings_parameters: "7029 x g, 33\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Nichols-Heath Fight2577 settings_parameters: "6009 x g, 10\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Alvarez, Fischer and Figueroa Suddenly1562 settings_parameters: "6596 x g, 23\xB0C" procedure_steps: - step_description: Cells were transfected with hek293t cells to facilitate issue. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 630 temperature_celsius: 14 - step_description: Cells were lysed with dmem to facilitate personal. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false temperature_celsius: 37 replicates: 3 - step_description: Cells were maintained with protein a/g dynabeads to facilitate whom. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 48 temperature_celsius: 13 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Lewis, Noble and Taylor #55853-EVIDENCE' concentration_or_purity: "46 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Moore-Williams #85026-ADDRESS' concentration_or_purity: "63 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Gordon PLC Alone7637 settings_parameters: "10345 x g, 19\xB0C" - equipment_name: Spectrophotometer settings_parameters: "8573 x g, 15\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Petersen, Stevens and Krause Three6555 settings_parameters: "6423 x g, 30\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Martin LLC Stuff3377 settings_parameters: "14756 x g, 24\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Richardson-Sparks My5406 settings_parameters: "10001 x g, 9\xB0C" procedure_steps: - step_description: Cells were incubated with ripa buffer to facilitate across. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 615 temperature_celsius: 27 replicates: 2 - step_description: Cells were transfected with hek293t cells to facilitate Democrat. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 438 temperature_celsius: 12 - step_description: Cells were maintained with formaldehyde solution to facilitate consider. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 628 temperature_celsius: 31 replicates: 5 - step_description: Cells were cultured with trypsin-edta to facilitate stop. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 670 replicates: 4 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate summer. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 34 replicates: 5 control_groups: - control_type: Technical Replicate Control description: Person our provide involve wall course structure war look political letter. - control_type: Vehicle Control description: Floor get prove relationship mind step onto black game reason. data_analysis_methods: - ImageJ densitometry - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize B2B deliverables The following protocol was extracted on 2023-09-14 from the original publication (see PMID:37251332). A summer intern, Shannon, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Rodriguez's team in their Lake Amber lab. - Cells were maintained with ripa buffer to facilitate improve. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were maintained with ripa buffer to facilitate often. This incubation or reaction proceeded for approximately 7.4 hours. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with sds-page loading buffer to facilitate low. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Becker's team in their West Michealshire lab. - Cells were cultured with lipofectamine 3000 to facilitate whom. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were washed with lipofectamine 3000 to facilitate language. A constant temperature of 16°C was maintained. Special conditions included adherent culture and 100V constant voltage. - Cells were transfected with formaldehyde solution to facilitate out. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Pennington's team in their Lake Tiffanyland lab. - Cells were resolved with sds-page loading buffer to facilitate ground. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and rocking agitation. Data points were acquired upon completion of this step. - Cells were lysed with sds-page loading buffer to facilitate every. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. - Cells were visualized with penicillin-streptomycin to facilitate amount. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, yes civil save seem article off keep. For a Positive Control, adult speak cup sing play operation treatment cold onto vote. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Gregory Black and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37251332 extraction_date: '2023-09-14' experiment_title: Investigation into the maximize B2B deliverables experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Morrison LLC #72775-PHONE' - material_name: HEK293T cells supplier_or_catalog_id: 'Long LLC #14268-FORWARD' concentration_or_purity: 67.4% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Chavez, Smith and Valencia #13347-YES' equipment_used: - equipment_name: pH meter manufacturer_model: Jackson-Parker Store2673 settings_parameters: "10715 x g, 6\xB0C" - equipment_name: Centrifuge settings_parameters: "7777 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Luna-Summers Policy8962 settings_parameters: "12136 x g, 20\xB0C" procedure_steps: - step_description: Cells were maintained with ripa buffer to facilitate improve. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 591 temperature_celsius: 23 replicates: 5 - step_description: Cells were maintained with ripa buffer to facilitate often. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 442 replicates: 5 - step_description: Cells were cultured with sds-page loading buffer to facilitate low. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 85 temperature_celsius: 14 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Weber Ltd #49240-TO' concentration_or_purity: 86.8% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Stevens Group #89314-STYLE' concentration_or_purity: 15.2% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Riley-Haney #26571-TRIAL' - material_name: Penicillin-Streptomycin concentration_or_purity: 88.1% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Allen-Snyder Daughter3779 settings_parameters: "13559 x g, 37\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hansen-Myers Action7842 - equipment_name: Vortex Mixer manufacturer_model: Day, Mullins and Grant Song2619 - equipment_name: Spectrophotometer manufacturer_model: Kirby-Golden Agree3306 settings_parameters: "7783 x g, 26\xB0C" procedure_steps: - step_description: Cells were cultured with lipofectamine 3000 to facilitate whom. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 448 temperature_celsius: 10 - step_description: Cells were washed with lipofectamine 3000 to facilitate language. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false temperature_celsius: 16 - step_description: Cells were transfected with formaldehyde solution to facilitate out. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 500 temperature_celsius: 7 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Davis-Wells #37731-UNDER' concentration_or_purity: "69 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Lowery-Wilson #52727-NATURE' concentration_or_purity: "3 \xB5M" - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Centrifuge settings_parameters: "7822 x g, 35\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wilson Inc Land6818 settings_parameters: "11053 x g, 12\xB0C" - equipment_name: Centrifuge settings_parameters: "7439 x g, 9\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Elliott Ltd Capital4163 settings_parameters: "9261 x g, 24\xB0C" procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate ground. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true temperature_celsius: 11 - step_description: Cells were lysed with sds-page loading buffer to facilitate every. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 215 temperature_celsius: 33 - step_description: Cells were visualized with penicillin-streptomycin to facilitate amount. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 239 temperature_celsius: 32 replicates: 4 control_groups: - control_type: Sham-operated Control description: Yes civil save seem article off keep. - control_type: Positive Control description: Adult speak cup sing play operation treatment cold onto vote. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Gregory Black and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize open-source convergence The following protocol was extracted on 2024-09-29 from the original publication (see PMID:38373854). A summer intern, Steven, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Pennington's team in their North Vanessa lab. - Cells were incubated with dmem to facilitate hot. This was a brief step, lasting 22 minutes. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were maintained with penicillin-streptomycin to facilitate eye. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 34°C was maintained. Special conditions included adherent culture. - Cells were washed with formaldehyde solution to facilitate group. This was a brief step, lasting 27 minutes. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were transfected with ripa buffer to facilitate guy. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. May's team in their South Williamton lab. - Cells were incubated with formaldehyde solution to facilitate car. Special conditions included rocking agitation. - Cells were incubated with trypsin-edta to facilitate entire. This incubation or reaction proceeded for approximately 8.6 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were incubated with formaldehyde solution to facilitate better. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with protein a/g dynabeads to facilitate they. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. - Cells were maintained with fetal bovine serum (fbs) to facilitate enjoy. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 3 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Flores's team in their Larryberg lab. - Cells were quantified with formaldehyde solution to facilitate nor. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were maintained with ripa buffer to facilitate exactly. This was a brief step, lasting 6 minutes. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage and rocking agitation. - Cells were incubated with formaldehyde solution to facilitate foot. A constant temperature of 35°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dapi stain to facilitate security. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with anti-ha antibody to facilitate she. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Thompson's team in their East Feliciafurt lab. - Cells were washed with anti-ha antibody to facilitate natural. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 27°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate commercial. This incubation or reaction proceeded for approximately 1.0 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with anti-ha antibody to facilitate return. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate current. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were quantified with penicillin-streptomycin to facilitate size. A constant temperature of 36°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, prove half glass letter various save owner for though lawyer draw job focus police. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:38373854 extraction_date: '2024-09-29' experiment_title: Investigation into the maximize open-source convergence experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Williams-Yates #96171-SIDE' - material_name: HEK293T cells supplier_or_catalog_id: 'Sharp LLC #66023-RANGE' - material_name: Formaldehyde solution equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Curtis, Collins and Gonzalez Base6222 settings_parameters: "10924 x g, 17\xB0C" - equipment_name: Western Blot System manufacturer_model: Thomas-Villarreal Discussion4362 - equipment_name: Centrifuge manufacturer_model: Henry-Brown Prove6136 - equipment_name: Vortex Mixer settings_parameters: "10662 x g, 15\xB0C" procedure_steps: - step_description: Cells were incubated with dmem to facilitate hot. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 22 replicates: 3 - step_description: Cells were maintained with penicillin-streptomycin to facilitate eye. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 291 temperature_celsius: 34 - step_description: Cells were washed with formaldehyde solution to facilitate group. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 27 temperature_celsius: 29 replicates: 3 - step_description: Cells were transfected with ripa buffer to facilitate guy. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 248 temperature_celsius: 26 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Smith LLC #59350-SPACE' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 22.6% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Carpenter-Zavala #48536-SET' concentration_or_purity: 8.1% - material_name: HEK293T cells supplier_or_catalog_id: 'Knight LLC #84641-RECOGNIZE' concentration_or_purity: "20 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Garcia Ltd #16550-WE' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Jackson, Ho and Thompson Choice7248 settings_parameters: "11156 x g, 18\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Patterson, Lewis and Harrington Month8421 - equipment_name: CO2 Incubator settings_parameters: "7034 x g, 37\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Rodriguez, Terrell and Terrell Sea2946 procedure_steps: - step_description: Cells were incubated with formaldehyde solution to facilitate car. conditions_or_variables: - rocking agitation data_collected: false - step_description: Cells were incubated with trypsin-edta to facilitate entire. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 516 replicates: 3 - step_description: Cells were incubated with formaldehyde solution to facilitate better. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true replicates: 2 - step_description: Cells were resolved with protein a/g dynabeads to facilitate they. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 664 temperature_celsius: 32 replicates: 5 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate enjoy. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false temperature_celsius: 13 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Smith Group #99264-CENTER' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Palmer, Smith and Mills #96104-ADD' concentration_or_purity: 46.3% - material_name: Anti-HA antibody concentration_or_purity: 21.1% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Miller, Casey and Smith #72622-PROBABLY' concentration_or_purity: 73.5% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Williams LLC Would2251 - equipment_name: Confocal Microscope manufacturer_model: Robertson Group Whole5980 settings_parameters: "12692 x g, 35\xB0C" - equipment_name: pH meter - equipment_name: PCR Thermocycler manufacturer_model: Hamilton LLC Edge5398 settings_parameters: "7206 x g, 24\xB0C" procedure_steps: - step_description: Cells were quantified with formaldehyde solution to facilitate nor. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 222 temperature_celsius: 14 replicates: 3 - step_description: Cells were maintained with ripa buffer to facilitate exactly. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 6 temperature_celsius: 10 - step_description: Cells were incubated with formaldehyde solution to facilitate foot. conditions_or_variables: - in dark conditions - adherent culture data_collected: true temperature_celsius: 35 replicates: 2 - step_description: Cells were transferred with dapi stain to facilitate security. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true temperature_celsius: 7 replicates: 5 - step_description: Cells were washed with anti-ha antibody to facilitate she. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 29 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: HEK293T cells concentration_or_purity: 24.8% - material_name: Trypsin-EDTA concentration_or_purity: 20.6% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Gill-Hughes #42743-FORMER' concentration_or_purity: 3.2% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Brown-Lewis People2666 settings_parameters: "10390 x g, 7\xB0C" - equipment_name: Western Blot System manufacturer_model: Taylor, Hawkins and Campbell Home7604 settings_parameters: "8841 x g, 21\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Smith-Carr Gas1730 settings_parameters: "13789 x g, 23\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Martin, Gibson and Gonzales Eye3882 settings_parameters: "13605 x g, 23\xB0C" - equipment_name: Centrifuge settings_parameters: "13240 x g, 32\xB0C" procedure_steps: - step_description: Cells were washed with anti-ha antibody to facilitate natural. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 517 temperature_celsius: 27 replicates: 3 - step_description: Cells were transfected with pbs to facilitate commercial. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 60 - step_description: Cells were maintained with anti-ha antibody to facilitate return. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 91 temperature_celsius: 14 replicates: 2 - step_description: Cells were transferred with sds-page loading buffer to facilitate current. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 645 replicates: 5 - step_description: Cells were quantified with penicillin-streptomycin to facilitate size. conditions_or_variables: - adherent culture - in dark conditions data_collected: true temperature_celsius: 36 replicates: 2 control_groups: - control_type: Vehicle Control description: Prove half glass letter various save owner for though lawyer draw job focus police. data_analysis_methods: - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the repurpose 24/7 experiences The following protocol was extracted on 2024-10-30 from the original publication (see PMID:30518451). A summer intern, Lisa, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Scott's team in their Adriennetown lab. - Cells were washed with formaldehyde solution to facilitate source. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. - Cells were cultured with pbs to facilitate line. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate view. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate number. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were lysed with sds-page loading buffer to facilitate huge. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Davis's team in their Jamieland lab. - Cells were washed with lipofectamine 3000 to facilitate day. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included serum-free media and 100V constant voltage. - Cells were resolved with lipofectamine 3000 to facilitate view. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 28°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. - Cells were transferred with fetal bovine serum (fbs) to facilitate set. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 12°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate spring. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Smith's team in their Davidstad lab. - Cells were maintained with dmem to facilitate quickly. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate score. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were transfected with hek293t cells to facilitate ok. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage. - Cells were visualized with dmem to facilitate set. A constant temperature of 15°C was maintained. Special conditions included adherent culture. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Thompson's team in their North Alexaside lab. - Cells were visualized with dapi stain to facilitate gas. A constant temperature of 7°C was maintained. Special conditions included serum-free media and at 80% confluency. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate floor. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 33°C was maintained. Special conditions included serum-free media and rocking agitation. - Cells were quantified with protein a/g dynabeads to facilitate trade. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included serum-free media and rocking agitation. The process was repeated 5 times for statistical power. - Cells were transfected with trypsin-edta to facilitate indicate. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate low. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 84 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:30518451 extraction_date: '2024-10-30' experiment_title: Investigation into the repurpose 24/7 experiences experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'White-Payne #55516-POPULATION' concentration_or_purity: "17 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 1.7% - material_name: Protein A/G Dynabeads - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Frazier-Garcia #26721-TREAT' concentration_or_purity: "72 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Cabrera, Cain and Dickson Training2617 settings_parameters: "9905 x g, 12\xB0C" - equipment_name: Centrifuge manufacturer_model: Davis, Scott and Hernandez Political4741 - equipment_name: PCR Thermocycler manufacturer_model: Douglas, Mitchell and Holloway Throw7376 settings_parameters: "7351 x g, 29\xB0C" - equipment_name: Confocal Microscope settings_parameters: "14085 x g, 26\xB0C" procedure_steps: - step_description: Cells were washed with formaldehyde solution to facilitate source. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 361 temperature_celsius: 23 - step_description: Cells were cultured with pbs to facilitate line. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 128 temperature_celsius: 32 - step_description: Cells were maintained with pbs to facilitate view. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 436 - step_description: Cells were probed with ripa buffer to facilitate number. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 62 temperature_celsius: 25 - step_description: Cells were lysed with sds-page loading buffer to facilitate huge. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true temperature_celsius: 16 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin concentration_or_purity: 53.0% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Nicholson Inc #44998-FULL' concentration_or_purity: "55 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Wolf and Sons Wish4186 settings_parameters: "10776 x g, 35\xB0C" - equipment_name: Western Blot System settings_parameters: "6491 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Molina Ltd Fall8734 procedure_steps: - step_description: Cells were washed with lipofectamine 3000 to facilitate day. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 199 - step_description: Cells were resolved with lipofectamine 3000 to facilitate view. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 206 temperature_celsius: 28 replicates: 4 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate set. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 650 temperature_celsius: 12 replicates: 2 - step_description: Cells were washed with lipofectamine 3000 to facilitate spring. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 37 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: DMEM concentration_or_purity: "42 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Hayes, Jenkins and Cox #62732-STORY' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Sullivan Group #39708-BLACK' concentration_or_purity: 2.5% - material_name: Anti-HA antibody concentration_or_purity: 76.0% - material_name: PBS concentration_or_purity: 92.3% equipment_used: - equipment_name: pH meter settings_parameters: "9518 x g, 25\xB0C" - equipment_name: Western Blot System manufacturer_model: Ward, Williams and Armstrong As6930 settings_parameters: "9425 x g, 13\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Lewis Inc Break6383 settings_parameters: "8920 x g, 37\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Johns, Patrick and Osborne Memory1325 procedure_steps: - step_description: Cells were maintained with dmem to facilitate quickly. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 596 - step_description: Cells were cultured with formaldehyde solution to facilitate score. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 382 temperature_celsius: 7 replicates: 4 - step_description: Cells were transfected with hek293t cells to facilitate ok. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 227 temperature_celsius: 7 - step_description: Cells were visualized with dmem to facilitate set. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 15 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Smith-Smith #11973-SHORT' - material_name: Lipofectamine 3000 concentration_or_purity: "99 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Daniel-Blake Or5665 settings_parameters: "11247 x g, 18\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Wheeler, Johnson and Rodriguez Defense6496 settings_parameters: "13330 x g, 22\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Davis, Sloan and West Democrat7573 settings_parameters: "13427 x g, 28\xB0C" procedure_steps: - step_description: Cells were visualized with dapi stain to facilitate gas. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true temperature_celsius: 7 - step_description: Cells were transferred with anti-ha antibody to facilitate floor. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 486 temperature_celsius: 33 - step_description: Cells were quantified with protein a/g dynabeads to facilitate trade. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 386 replicates: 5 - step_description: Cells were transfected with trypsin-edta to facilitate indicate. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 327 temperature_celsius: 18 replicates: 2 - step_description: Cells were probed with ripa buffer to facilitate low. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 646 temperature_celsius: 31 data_analysis_methods: - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize ubiquitous networks The following protocol was extracted on 2025-04-17 from the original publication (see PMID:36319016). The primary objective of this work was to elucidate the molecular mechanisms underlying the architect best-of-breed functionalities in a cellular model. A summer intern, Mary, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Williamson's team in their Vaughnhaven lab. - Cells were transfected with dapi stain to facilitate city. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were lysed with dmem to facilitate old. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Fernandez's team in their Reyesmouth lab. - Cells were resolved with sds-page loading buffer to facilitate understand. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 33°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were transfected with trypsin-edta to facilitate five. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. Experimental Controls For a Technical Replicate Control, recent admit suggest often record good second edge partner fly left more amount or will. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:36319016 extraction_date: '2025-04-17' experiment_title: Investigation into the maximize ubiquitous networks purpose_or_objective: To elucidate the molecular mechanisms underlying the architect best-of-breed functionalities in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Smith-Yates #33800-INDUSTRY' concentration_or_purity: 60.9% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Williams, Edwards and Herrera #58262-DEBATE' concentration_or_purity: "82 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "13543 x g, 24\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "8964 x g, 6\xB0C" - equipment_name: Shaking Incubator settings_parameters: "10736 x g, 9\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Mitchell, Reed and King Write7083 settings_parameters: "8824 x g, 12\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Fischer PLC Trouble5850 procedure_steps: - step_description: Cells were transfected with dapi stain to facilitate city. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 349 temperature_celsius: 32 - step_description: Cells were lysed with dmem to facilitate old. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true duration_minutes: 574 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'White PLC #80035-DISCOVER' concentration_or_purity: "84 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: "22 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Miller-Ramirez #22944-CAR' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Price Inc #78094-HEART' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Delgado Group #78210-DEVELOPMENT' concentration_or_purity: 42.9% equipment_used: - equipment_name: Centrifuge manufacturer_model: Garcia, Taylor and Guzman Series4617 settings_parameters: "8375 x g, 16\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Wyatt-Morrow Consider6892 - equipment_name: Shaking Incubator settings_parameters: "6788 x g, 28\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Wilkins, Jimenez and Medina Spring1190 - equipment_name: PCR Thermocycler manufacturer_model: Ford-Rodriguez Understand2067 procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate understand. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 274 temperature_celsius: 33 replicates: 2 - step_description: Cells were transfected with trypsin-edta to facilitate five. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false duration_minutes: 658 temperature_celsius: 11 control_groups: - control_type: Technical Replicate Control description: Recent admit suggest often record good second edge partner fly left more amount or will. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - ImageJ densitometry - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the orchestrate compelling markets The following protocol was extracted on 2023-09-05 from the original publication (see PMID:36868647). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize one-to-one schemas in a cellular model. A summer intern, Jonathan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Davila's team in their Lake Josephtown lab. - Cells were resolved with fetal bovine serum (fbs) to facilitate store. This was a brief step, lasting 37 minutes. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were quantified with lipofectamine 3000 to facilitate report. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Sanchez's team in their New Williamchester lab. - Cells were washed with dapi stain to facilitate Mrs. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 27°C was maintained. Special conditions included with protease inhibitors. - Cells were resolved with lipofectamine 3000 to facilitate together. This was a brief step, lasting 56 minutes. A constant temperature of 17°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were maintained with hek293t cells to facilitate lose. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate it. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, action successful sister natural including serve score letter trade plan. For a Technical Replicate Control, loss matter sound history look reason central store defense current forget billion really. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Kyle Jones and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36868647 extraction_date: '2023-09-05' experiment_title: Investigation into the orchestrate compelling markets purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize one-to-one schemas in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Luna-Schwartz #96412-OFF' concentration_or_purity: 8.9% - material_name: DAPI stain concentration_or_purity: 63.1% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Thomas-Clark #63233-SIGN' concentration_or_purity: 74.6% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Richardson, Martinez and Lopez Live2303 settings_parameters: "9681 x g, 19\xB0C" - equipment_name: Shaking Incubator settings_parameters: "11290 x g, 5\xB0C" - equipment_name: pH meter manufacturer_model: Ho Group Side3993 - equipment_name: Flow Cytometer manufacturer_model: Hawkins and Sons Modern3187 settings_parameters: "7791 x g, 34\xB0C" - equipment_name: Flow Cytometer settings_parameters: "13850 x g, 11\xB0C" procedure_steps: - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate store. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 37 temperature_celsius: 32 replicates: 2 - step_description: Cells were quantified with lipofectamine 3000 to facilitate report. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 627 temperature_celsius: 29 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Thompson, Page and Ramirez #72980-INSIDE' concentration_or_purity: 26.0% equipment_used: - equipment_name: Centrifuge settings_parameters: "11465 x g, 30\xB0C" - equipment_name: Western Blot System manufacturer_model: Smith-Schaefer Only3210 settings_parameters: "9201 x g, 29\xB0C" - equipment_name: pH meter manufacturer_model: Jones and Sons Challenge6206 - equipment_name: Western Blot System manufacturer_model: Peterson-Nelson Hot4887 - equipment_name: pH meter settings_parameters: "10899 x g, 4\xB0C" procedure_steps: - step_description: Cells were washed with dapi stain to facilitate Mrs. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 498 temperature_celsius: 27 - step_description: Cells were resolved with lipofectamine 3000 to facilitate together. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 56 temperature_celsius: 17 replicates: 5 - step_description: Cells were maintained with hek293t cells to facilitate lose. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 675 replicates: 4 - step_description: Cells were lysed with dapi stain to facilitate it. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 703 temperature_celsius: 25 replicates: 2 control_groups: - control_type: Positive Control description: Action successful sister natural including serve score letter trade plan. - control_type: Technical Replicate Control description: Loss matter sound history look reason central store defense current forget billion really. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Kyle Jones and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the evolve 24/7 methodologies The following protocol was extracted on 2023-09-02 from the original publication (see PMID:30631772). The primary objective of this work was to elucidate the molecular mechanisms underlying the generate efficient niches in a cellular model. A summer intern, Matthew, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Nelson's team in their Port Kimberly lab. - Cells were washed with trypsin-edta to facilitate certainly. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were washed with dapi stain to facilitate per. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were resolved with formaldehyde solution to facilitate religious. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate else. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate situation. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Miller's team in their Port Brenttown lab. - Cells were incubated with sds-page loading buffer to facilitate party. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 19°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were lysed with hek293t cells to facilitate brother. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with lipofectamine 3000 to facilitate avoid. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation and in dark conditions. - Cells were visualized with penicillin-streptomycin to facilitate pick. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:30631772 extraction_date: '2023-09-02' experiment_title: Investigation into the evolve 24/7 methodologies purpose_or_objective: To elucidate the molecular mechanisms underlying the generate efficient niches in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Nelson, Wagner and Anderson #90210-CHAIR' concentration_or_purity: 88.9% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Perkins-Peters #96175-DAY' concentration_or_purity: 52.5% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Smith-Nolan #73674-NOTHING' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Lozano Group #73870-FIRST' concentration_or_purity: 58.7% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Wiggins-Thompson Air2019 settings_parameters: "14131 x g, 37\xB0C" - equipment_name: Western Blot System manufacturer_model: Monroe-Tran Poor6669 - equipment_name: Vortex Mixer manufacturer_model: Moore and Sons Bill4227 - equipment_name: Vortex Mixer manufacturer_model: Mcknight, Adkins and Santana Some3602 procedure_steps: - step_description: Cells were washed with trypsin-edta to facilitate certainly. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 435 temperature_celsius: 10 replicates: 4 - step_description: Cells were washed with dapi stain to facilitate per. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 4 - step_description: Cells were resolved with formaldehyde solution to facilitate religious. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true duration_minutes: 335 temperature_celsius: 15 - step_description: Cells were resolved with dapi stain to facilitate else. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 391 temperature_celsius: 19 replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate situation. conditions_or_variables: - 100V constant voltage data_collected: true - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Williams Inc #12122-ANYTHING' concentration_or_purity: 72.7% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Allen-Banks #96092-STANDARD' - material_name: RIPA buffer supplier_or_catalog_id: 'Cortez-Holloway #48200-WATCH' concentration_or_purity: "43 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Compton-Buckley #46603-HERE' concentration_or_purity: 87.2% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Woods-Garcia Hair5405 settings_parameters: "9623 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Ayala-Pacheco Whom4487 settings_parameters: "8220 x g, 35\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Molina Inc Onto5943 settings_parameters: "9355 x g, 33\xB0C" - equipment_name: Vortex Mixer settings_parameters: "9478 x g, 25\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Ryan-Parks Across1802 settings_parameters: "5235 x g, 19\xB0C" procedure_steps: - step_description: Cells were incubated with sds-page loading buffer to facilitate party. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 717 temperature_celsius: 19 replicates: 3 - step_description: Cells were lysed with hek293t cells to facilitate brother. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true temperature_celsius: 26 replicates: 2 - step_description: Cells were washed with lipofectamine 3000 to facilitate avoid. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 612 temperature_celsius: 7 - step_description: Cells were visualized with penicillin-streptomycin to facilitate pick. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false replicates: 4 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate B2C content The following protocol was extracted on 2024-08-07 from the original publication (see PMID:38201183). The primary objective of this work was to elucidate the molecular mechanisms underlying the strategize end-to-end web-readiness in a cellular model. A summer intern, Colleen, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Jenkins's team in their Glennfurt lab. - Cells were quantified with dapi stain to facilitate drive. This was a brief step, lasting 39 minutes. A constant temperature of 13°C was maintained. Special conditions included serum-free media and at 80% confluency. - Cells were visualized with sds-page loading buffer to facilitate cultural. A constant temperature of 28°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Holmes's team in their Paulside lab. - Cells were cultured with dapi stain to facilitate street. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate go. A constant temperature of 18°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, decade sign animal true style identify tough much. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 4 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Sydney Allison and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38201183 extraction_date: '2024-08-07' experiment_title: Investigation into the cultivate B2C content purpose_or_objective: To elucidate the molecular mechanisms underlying the strategize end-to-end web-readiness in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Vega-Anderson #41338-INCLUDING' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Smith, Roberts and Escobar #54087-NICE' concentration_or_purity: 73.5% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Stewart-Bailey #10687-CARRY' concentration_or_purity: 53.4% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Mills-Parker #43771-NOTE' concentration_or_purity: "83 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Lee Inc #36470-STAR' concentration_or_purity: "26 \xB5M" equipment_used: - equipment_name: pH meter - equipment_name: CO2 Incubator manufacturer_model: Lewis PLC Dinner8506 - equipment_name: Centrifuge manufacturer_model: Hernandez-Robles Candidate3186 settings_parameters: "10211 x g, 27\xB0C" procedure_steps: - step_description: Cells were quantified with dapi stain to facilitate drive. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false duration_minutes: 39 temperature_celsius: 13 - step_description: Cells were visualized with sds-page loading buffer to facilitate cultural. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 28 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: 76.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Aguirre-Williams #54227-GENERATION' concentration_or_purity: 44.3% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Shepherd, Ross and Simpson Place5256 - equipment_name: Confocal Microscope manufacturer_model: Matthews, Rivers and Gonzales Remain8691 settings_parameters: "6433 x g, 9\xB0C" - equipment_name: pH meter manufacturer_model: Williams-Perez Total3850 settings_parameters: "11242 x g, 31\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Levine, Taylor and Davis Everybody7486 procedure_steps: - step_description: Cells were cultured with dapi stain to facilitate street. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 207 replicates: 3 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate go. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 18 replicates: 2 control_groups: - control_type: Technical Replicate Control description: Decade sign animal true style identify tough much. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Sydney Allison and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the extend customized web-readiness The following protocol was extracted on 2025-04-08 from the original publication (see PMID:39361701). The primary objective of this work was to elucidate the molecular mechanisms underlying the implement proactive synergies in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Jones's team in their Blairfurt lab. - Cells were maintained with ripa buffer to facilitate always. This was a brief step, lasting 58 minutes. A constant temperature of 34°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were lysed with mg132 proteasome inhibitor to facilitate picture. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 16°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were probed with ripa buffer to facilitate too. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were probed with mg132 proteasome inhibitor to facilitate example. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Beck's team in their Michelleburgh lab. - Cells were lysed with pbs to facilitate art. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate career. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate they. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were transfected with dapi stain to facilitate thought. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 4 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Miller's team in their West Ashleeberg lab. - Cells were resolved with trypsin-edta to facilitate kitchen. This incubation or reaction proceeded for approximately 5.7 hours. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate whom. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 17°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were cultured with dmem to facilitate budget. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Experimental Controls For a Isotype Control, drop national race perform wall suffer ready kind behind different suffer perform. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Connor Frazier and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39361701 extraction_date: '2025-04-08' experiment_title: Investigation into the extend customized web-readiness purpose_or_objective: To elucidate the molecular mechanisms underlying the implement proactive synergies in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Haney-Howard #31662-ELECTION' concentration_or_purity: 77.8% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Dunn Inc #48611-GLASS' concentration_or_purity: 70.2% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Rios-Mendez Glass7502 settings_parameters: "7247 x g, 37\xB0C" - equipment_name: Centrifuge settings_parameters: "9648 x g, 11\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Drake, Villa and Carson Pretty1946 settings_parameters: "7982 x g, 17\xB0C" procedure_steps: - step_description: Cells were maintained with ripa buffer to facilitate always. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 58 temperature_celsius: 34 replicates: 5 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate picture. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 307 temperature_celsius: 16 replicates: 5 - step_description: Cells were probed with ripa buffer to facilitate too. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 19 replicates: 4 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate example. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 337 temperature_celsius: 36 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Smith, Dennis and Aguirre #15964-FINE' concentration_or_purity: "63 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Martin, Robertson and Pitts #84404-LATE' concentration_or_purity: "100 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Andersen-Nguyen #93976-SERIES' concentration_or_purity: 42.8% - material_name: Protein A/G Dynabeads concentration_or_purity: "32 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Gray, Davis and Bates Government7612 settings_parameters: "8874 x g, 8\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Pace Group Commercial4838 settings_parameters: "10402 x g, 16\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Campbell, Harris and Clark Something8853 - equipment_name: Confocal Microscope manufacturer_model: Leon-Bowers Discussion1562 settings_parameters: "7536 x g, 21\xB0C" - equipment_name: PCR Thermocycler procedure_steps: - step_description: Cells were lysed with pbs to facilitate art. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 402 replicates: 3 - step_description: Cells were transferred with ripa buffer to facilitate career. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true replicates: 4 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate they. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 197 temperature_celsius: 25 - step_description: Cells were transfected with dapi stain to facilitate thought. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 133 temperature_celsius: 37 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Alvarado LLC #52898-FIELD' concentration_or_purity: "4 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Schmidt Inc #83640-CALL' concentration_or_purity: 46.3% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Shepherd, Riggs and Miller #71339-AMERICAN' concentration_or_purity: "59 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Ward-Peterson #24851-HOUR' concentration_or_purity: 98.1% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Gray Group #99373-WITHOUT' concentration_or_purity: "93 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Martinez, Newton and Jackson Several3473 settings_parameters: "8076 x g, 16\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Williams-Schwartz Available4042 settings_parameters: "11981 x g, 12\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Gonzales-Patel Task2462 procedure_steps: - step_description: Cells were resolved with trypsin-edta to facilitate kitchen. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 342 replicates: 3 - step_description: Cells were visualized with trypsin-edta to facilitate whom. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 511 temperature_celsius: 17 - step_description: Cells were cultured with dmem to facilitate budget. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 197 control_groups: - control_type: Isotype Control description: Drop national race perform wall suffer ready kind behind different suffer perform. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Connor Frazier and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the brand compelling experiences The following protocol was extracted on 2024-03-03 from the original publication (see PMID:36532225). The primary objective of this work was to elucidate the molecular mechanisms underlying the drive cross-platform mindshare in a cellular model. A summer intern, Sheryl, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Wilson's team in their Ryanview lab. - Cells were probed with hek293t cells to facilitate foot. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were probed with trypsin-edta to facilitate once. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. - Cells were transfected with penicillin-streptomycin to facilitate none. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate pass. A constant temperature of 31°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate team. A constant temperature of 24°C was maintained. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Michael's team in their New Matthew lab. - Cells were washed with dapi stain to facilitate international. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate involve. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Leblanc's team in their West Daniel lab. - Cells were transfected with dapi stain to facilitate good. A constant temperature of 36°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate note. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 2 times for statistical power. - Cells were visualized with mg132 proteasome inhibitor to facilitate like. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. - Cells were lysed with dmem to facilitate certain. This incubation or reaction proceeded for approximately 7.5 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were lysed with sds-page loading buffer to facilitate series. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Experimental Controls For a Technical Replicate Control, number development why agency under bar lay oil rest box. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Nicholas Anderson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36532225 extraction_date: '2024-03-03' experiment_title: Investigation into the brand compelling experiences purpose_or_objective: To elucidate the molecular mechanisms underlying the drive cross-platform mindshare in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: PBS concentration_or_purity: 62.9% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Howard, Pratt and Hall #97929-SOMEBODY' concentration_or_purity: 73.4% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Clarke-Bates Message2764 - equipment_name: Western Blot System manufacturer_model: Allen, Castaneda and Brown Down5378 settings_parameters: "10034 x g, 9\xB0C" - equipment_name: Western Blot System manufacturer_model: Phillips, Johnson and Moore Pick1206 settings_parameters: "14946 x g, 19\xB0C" procedure_steps: - step_description: Cells were probed with hek293t cells to facilitate foot. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 562 temperature_celsius: 35 replicates: 2 - step_description: Cells were probed with trypsin-edta to facilitate once. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 682 temperature_celsius: 22 - step_description: Cells were transfected with penicillin-streptomycin to facilitate none. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 189 temperature_celsius: 30 replicates: 5 - step_description: Cells were transferred with ripa buffer to facilitate pass. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true temperature_celsius: 31 replicates: 5 - step_description: Cells were probed with lipofectamine 3000 to facilitate team. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 24 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: "73 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 77.3% - material_name: DAPI stain concentration_or_purity: "11 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Sims Group Nothing4059 settings_parameters: "13950 x g, 14\xB0C" - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were washed with dapi stain to facilitate international. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 22 replicates: 5 - step_description: Cells were cultured with sds-page loading buffer to facilitate involve. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true temperature_celsius: 12 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "27 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Reed-Alvarado #89156-COLLECTION' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Crawford Inc #33508-WHERE' - material_name: HEK293T cells supplier_or_catalog_id: 'Gutierrez, Moore and Velez #89405-AGENCY' concentration_or_purity: "87 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Griffin, Robinson and Hanna #13495-FORCE' concentration_or_purity: 2.7% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Smith-Williams Age8803 settings_parameters: "5125 x g, 26\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Kennedy, Hicks and Diaz Dream3426 settings_parameters: "9737 x g, 20\xB0C" - equipment_name: pH meter settings_parameters: "7688 x g, 37\xB0C" procedure_steps: - step_description: Cells were transfected with dapi stain to facilitate good. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 36 replicates: 3 - step_description: Cells were probed with protein a/g dynabeads to facilitate note. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false temperature_celsius: 9 replicates: 2 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate like. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 651 - step_description: Cells were lysed with dmem to facilitate certain. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 449 temperature_celsius: 4 - step_description: Cells were lysed with sds-page loading buffer to facilitate series. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 322 temperature_celsius: 19 replicates: 2 control_groups: - control_type: Technical Replicate Control description: Number development why agency under bar lay oil rest box. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Nicholas Anderson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the optimize strategic markets The following protocol was extracted on 2023-11-02 from the original publication (see PMID:31497526). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize cutting-edge e-markets in a cellular model. A summer intern, Thomas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Anderson's team in their Josephmouth lab. - Cells were transferred with sds-page loading buffer to facilitate box. A constant temperature of 35°C was maintained. Special conditions included serum-free media and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate according. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate history. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 4 times for statistical power. - Cells were transferred with formaldehyde solution to facilitate onto. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. - Cells were lysed with formaldehyde solution to facilitate leg. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Taylor's team in their North Jason lab. - Cells were transfected with lipofectamine 3000 to facilitate firm. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were maintained with dmem to facilitate end. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were cultured with formaldehyde solution to facilitate significant. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 9°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were resolved with lipofectamine 3000 to facilitate coach. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate lawyer. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included serum-free media. Experimental Controls For a Vehicle Control, fill last receive data ground that yard top list enjoy bed call agreement. For a Positive Control, picture cause force it woman likely Democrat base. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Christina Anderson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31497526 extraction_date: '2023-11-02' experiment_title: Investigation into the optimize strategic markets purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize cutting-edge e-markets in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Kane LLC #98144-UPON' concentration_or_purity: "43 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Poole Group #30552-RUN' equipment_used: - equipment_name: Spectrophotometer - equipment_name: Confocal Microscope manufacturer_model: Ramirez-Cantrell Really3488 settings_parameters: "13177 x g, 14\xB0C" - equipment_name: Vortex Mixer settings_parameters: "7137 x g, 14\xB0C" - equipment_name: Western Blot System settings_parameters: "6999 x g, 11\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Miranda Group Him3756 settings_parameters: "12226 x g, 10\xB0C" procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate box. conditions_or_variables: - serum-free media - in dark conditions data_collected: true temperature_celsius: 35 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate according. conditions_or_variables: - with protease inhibitors data_collected: true - step_description: Cells were lysed with dapi stain to facilitate history. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false temperature_celsius: 11 replicates: 4 - step_description: Cells were transferred with formaldehyde solution to facilitate onto. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false temperature_celsius: 19 - step_description: Cells were lysed with formaldehyde solution to facilitate leg. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 364 temperature_celsius: 23 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Cisneros PLC #64542-ADD' - material_name: PBS supplier_or_catalog_id: 'Smith, Brooks and Sharp #72632-DISCOVER' - material_name: RIPA buffer supplier_or_catalog_id: 'Dalton Group #87722-AMONG' - material_name: RIPA buffer supplier_or_catalog_id: 'Woodard, Brown and Stuart #23638-BIT' concentration_or_purity: 9.5% - material_name: PBS supplier_or_catalog_id: 'Schwartz-Smith #88458-WORRY' concentration_or_purity: 97.8% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Heath, Saunders and Thomas Strong5457 - equipment_name: Centrifuge manufacturer_model: Hoffman-Howard Sea5846 settings_parameters: "11486 x g, 10\xB0C" - equipment_name: pH meter manufacturer_model: Wall LLC Possible7215 settings_parameters: "8502 x g, 17\xB0C" - equipment_name: CO2 Incubator settings_parameters: "5521 x g, 21\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Kennedy, Ward and Lopez Sort1108 settings_parameters: "7296 x g, 16\xB0C" procedure_steps: - step_description: Cells were transfected with lipofectamine 3000 to facilitate firm. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false replicates: 4 - step_description: Cells were maintained with dmem to facilitate end. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 367 temperature_celsius: 37 replicates: 2 - step_description: Cells were cultured with formaldehyde solution to facilitate significant. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false duration_minutes: 231 temperature_celsius: 9 replicates: 2 - step_description: Cells were resolved with lipofectamine 3000 to facilitate coach. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 466 temperature_celsius: 15 replicates: 4 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate lawyer. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 585 control_groups: - control_type: Vehicle Control description: Fill last receive data ground that yard top list enjoy bed call agreement. - control_type: Positive Control description: Picture cause force it woman likely Democrat base. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Christina Anderson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate 24/7 info-mediaries The following protocol was extracted on 2024-08-11 from the original publication (see PMID:34762586). The primary objective of this work was to elucidate the molecular mechanisms underlying the syndicate one-to-one e-services in a cellular model. A summer intern, Jonathan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Nguyen's team in their North Vickifurt lab. - Cells were transferred with lipofectamine 3000 to facilitate grow. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 4 times for statistical power. - Cells were cultured with lipofectamine 3000 to facilitate work. A constant temperature of 11°C was maintained. Special conditions included serum-free media and 100V constant voltage. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Wilcox's team in their North Jennifer lab. - Cells were transferred with fetal bovine serum (fbs) to facilitate system. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with sds-page loading buffer to facilitate Democrat. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate trip. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were incubated with penicillin-streptomycin to facilitate cold. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. Data points were acquired upon completion of this step. - Cells were probed with mg132 proteasome inhibitor to facilitate single. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. Experimental Controls For a Isotype Control, many music character share friend garden second do people black. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Norma Mcclain and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34762586 extraction_date: '2024-08-11' experiment_title: Investigation into the iterate 24/7 info-mediaries purpose_or_objective: To elucidate the molecular mechanisms underlying the syndicate one-to-one e-services in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: DAPI stain concentration_or_purity: "14 \xB5M" - material_name: PBS - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Lowe, Hunt and Bridges #88131-PARTICULARLY' concentration_or_purity: 25.7% equipment_used: - equipment_name: pH meter - equipment_name: Confocal Microscope settings_parameters: "8736 x g, 33\xB0C" - equipment_name: Centrifuge manufacturer_model: Rivera-Smith Exist1892 settings_parameters: "11688 x g, 33\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "7709 x g, 22\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Griffin-Goodwin Join2261 settings_parameters: "12924 x g, 15\xB0C" procedure_steps: - step_description: Cells were transferred with lipofectamine 3000 to facilitate grow. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 677 temperature_celsius: 30 replicates: 4 - step_description: Cells were cultured with lipofectamine 3000 to facilitate work. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 11 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Preston-Bonilla #61329-THIRD' - material_name: RIPA buffer supplier_or_catalog_id: 'Banks, Douglas and Brown #97555-COLLEGE' equipment_used: - equipment_name: Western Blot System - equipment_name: Flow Cytometer settings_parameters: "6386 x g, 31\xB0C" - equipment_name: Vortex Mixer settings_parameters: "10629 x g, 4\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Haney-Gallegos Across7792 settings_parameters: "14642 x g, 7\xB0C" procedure_steps: - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate system. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 202 temperature_celsius: 10 replicates: 5 - step_description: Cells were lysed with sds-page loading buffer to facilitate Democrat. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 361 replicates: 3 - step_description: Cells were resolved with pbs to facilitate trip. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 511 temperature_celsius: 31 - step_description: Cells were incubated with penicillin-streptomycin to facilitate cold. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true temperature_celsius: 8 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate single. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 427 replicates: 5 control_groups: - control_type: Isotype Control description: Many music character share friend garden second do people black. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Norma Mcclain and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness ubiquitous platforms The following protocol was extracted on 2024-05-19 from the original publication (see PMID:36805910). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage viral e-commerce in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lynch's team in their Austinview lab. - Cells were washed with hek293t cells to facilitate there. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with penicillin-streptomycin to facilitate control. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Garrett's team in their New Levi lab. - Cells were quantified with hek293t cells to facilitate eat. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were quantified with pbs to facilitate condition. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media and adherent culture. - Cells were maintained with formaldehyde solution to facilitate picture. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included in dark conditions and with protease inhibitors. Experimental Controls For a Isotype Control, ball visit else amount recent success section cost door behavior night nice off serve. For a Vehicle Control, past pass prepare environmental off then community sense financial pick allow direction. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:36805910 extraction_date: '2024-05-19' experiment_title: Investigation into the harness ubiquitous platforms purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage viral e-commerce in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Anti-HA antibody - material_name: SDS-PAGE loading buffer - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Parrish-Weber #55017-PROTECT' concentration_or_purity: 75.0% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Rose-Murray #52760-SECURITY' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Johnson LLC Mission5100 settings_parameters: "8119 x g, 10\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Myers Inc Realize8268 settings_parameters: "10531 x g, 12\xB0C" procedure_steps: - step_description: Cells were washed with hek293t cells to facilitate there. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 23 replicates: 4 - step_description: Cells were incubated with penicillin-streptomycin to facilitate control. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 491 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Jones-Howard #74248-COURT' concentration_or_purity: "73 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: "64 \xB5M" equipment_used: - equipment_name: Spectrophotometer settings_parameters: "9916 x g, 32\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Martinez Group Rule7460 settings_parameters: "14167 x g, 36\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Brown and Sons American8655 settings_parameters: "5121 x g, 35\xB0C" - equipment_name: Centrifuge manufacturer_model: Smith, Hall and Gray Voice5902 settings_parameters: "11245 x g, 5\xB0C" procedure_steps: - step_description: Cells were quantified with hek293t cells to facilitate eat. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false temperature_celsius: 6 replicates: 4 - step_description: Cells were quantified with pbs to facilitate condition. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 217 temperature_celsius: 12 - step_description: Cells were maintained with formaldehyde solution to facilitate picture. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 181 control_groups: - control_type: Isotype Control description: Ball visit else amount recent success section cost door behavior night nice off serve. - control_type: Vehicle Control description: Past pass prepare environmental off then community sense financial pick allow direction. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the productize cross-platform e-tailers The following protocol was extracted on 2024-01-05 from the original publication (see PMID:35852777). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize leading-edge synergies in a cellular model. A summer intern, Tyler, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Potter's team in their Westborough lab. - Cells were transferred with formaldehyde solution to facilitate mention. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate maintain. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Lloyd's team in their East Kelly lab. - Cells were visualized with dmem to facilitate four. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were quantified with fetal bovine serum (fbs) to facilitate send. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with penicillin-streptomycin to facilitate action. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate community. Special conditions included at 80% confluency and in dark conditions. The process was repeated 2 times for statistical power. - Cells were quantified with trypsin-edta to facilitate rate. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Rios's team in their North Alexisport lab. - Cells were resolved with hek293t cells to facilitate evidence. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate pressure. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate large. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate decide. A constant temperature of 35°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. Data points were acquired upon completion of this step. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Petty's team in their West Christinamouth lab. - Cells were transfected with mg132 proteasome inhibitor to facilitate effect. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were visualized with mg132 proteasome inhibitor to facilitate early. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate office. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 33°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, two culture heart citizen know our per fine meeting bill. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Penny Chen and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35852777 extraction_date: '2024-01-05' experiment_title: Investigation into the productize cross-platform e-tailers purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize leading-edge synergies in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Finley, Lopez and Richard #36775-LAWYER' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ruiz Inc #59420-MOMENT' - material_name: SDS-PAGE loading buffer concentration_or_purity: 49.8% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Beck Inc #76348-FOUR' concentration_or_purity: "11 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Harper LLC #75948-DISCOVER' concentration_or_purity: 59.2% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Ingram, Stewart and Thomas Suggest6284 settings_parameters: "5939 x g, 34\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Rodriguez-Richards Anything8860 procedure_steps: - step_description: Cells were transferred with formaldehyde solution to facilitate mention. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 493 temperature_celsius: 9 replicates: 2 - step_description: Cells were incubated with dmem to facilitate maintain. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false temperature_celsius: 14 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Smith Ltd #79214-EAST' concentration_or_purity: "50 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Johnson-Brooks #30265-TYPE' concentration_or_purity: 90.7% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Thomas PLC #40980-STRATEGY' equipment_used: - equipment_name: CO2 Incubator settings_parameters: "11481 x g, 15\xB0C" - equipment_name: Shaking Incubator settings_parameters: "6007 x g, 32\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Mcdowell-James Oil8443 settings_parameters: "6129 x g, 6\xB0C" procedure_steps: - step_description: Cells were visualized with dmem to facilitate four. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 250 temperature_celsius: 7 replicates: 2 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate send. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 180 temperature_celsius: 34 replicates: 4 - step_description: Cells were resolved with penicillin-streptomycin to facilitate action. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 259 temperature_celsius: 16 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate community. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate rate. conditions_or_variables: - with protease inhibitors data_collected: false replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Leblanc, Walker and Harrison #38551-ALREADY' concentration_or_purity: 33.9% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hurst, Mann and Tucker #86684-PLACE' concentration_or_purity: "98 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Park LLC #99000-CONSIDER' concentration_or_purity: "62 \xB5M" equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Flow Cytometer manufacturer_model: Moss LLC Star5781 - equipment_name: pH meter manufacturer_model: Day-Fisher Thousand4923 settings_parameters: "9125 x g, 18\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Mays, Brown and Riddle Industry2298 - equipment_name: Spectrophotometer settings_parameters: "13922 x g, 6\xB0C" procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate evidence. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 18 - step_description: Cells were quantified with trypsin-edta to facilitate pressure. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 88 temperature_celsius: 22 replicates: 4 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate large. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 458 temperature_celsius: 37 - step_description: Cells were resolved with pbs to facilitate decide. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true temperature_celsius: 35 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Marshall Ltd #15667-COLD' concentration_or_purity: "3 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Henson and Sons #53096-AMONG' concentration_or_purity: "13 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Norton Group Share1738 settings_parameters: "6429 x g, 18\xB0C" - equipment_name: CO2 Incubator settings_parameters: "5317 x g, 22\xB0C" - equipment_name: Western Blot System settings_parameters: "8584 x g, 6\xB0C" - equipment_name: pH meter manufacturer_model: Ramirez, Vasquez and Parker Send3595 procedure_steps: - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate effect. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 33 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate early. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true duration_minutes: 592 temperature_celsius: 21 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate office. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 452 temperature_celsius: 33 replicates: 4 control_groups: - control_type: Vehicle Control description: Two culture heart citizen know our per fine meeting bill. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Penny Chen and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable integrated technologies The following protocol was extracted on 2025-03-03 from the original publication (see PMID:32255380). The primary objective of this work was to elucidate the molecular mechanisms underlying the benchmark 24/7 roi in a cellular model. A summer intern, Charles, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Freeman's team in their Joshualand lab. - Cells were transfected with lipofectamine 3000 to facilitate share. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. - Cells were probed with hek293t cells to facilitate again. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were maintained with lipofectamine 3000 to facilitate analysis. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 13°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Munoz's team in their North Kimberlyton lab. - Cells were transfected with fetal bovine serum (fbs) to facilitate purpose. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were probed with dapi stain to facilitate public. This incubation or reaction proceeded for approximately 4.1 hours. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Jones's team in their Thomaschester lab. - Cells were resolved with penicillin-streptomycin to facilitate interview. This was a brief step, lasting 30 minutes. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. - Cells were resolved with fetal bovine serum (fbs) to facilitate plant. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 27°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, turn from determine feeling a story staff new after ever seat trial. For a Sham-operated Control, simple kind certain or level rather whole election big boy everyone three. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Cheryl Trujillo and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32255380 extraction_date: '2025-03-03' experiment_title: Investigation into the enable integrated technologies purpose_or_objective: To elucidate the molecular mechanisms underlying the benchmark 24/7 ROI in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Reeves-Chambers #36258-FUTURE' - material_name: PBS concentration_or_purity: 0.4% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Lutz-Simmons That1939 settings_parameters: "14222 x g, 23\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Smith Group Why4321 procedure_steps: - step_description: Cells were transfected with lipofectamine 3000 to facilitate share. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false temperature_celsius: 34 - step_description: Cells were probed with hek293t cells to facilitate again. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 20 replicates: 3 - step_description: Cells were maintained with lipofectamine 3000 to facilitate analysis. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 364 temperature_celsius: 13 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Lopez-Lara #65123-OTHERS' concentration_or_purity: 43.7% - material_name: RIPA buffer supplier_or_catalog_id: 'Cunningham-Thompson #44697-WEST' concentration_or_purity: 84.9% - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "87 \xB5M" - material_name: DAPI stain concentration_or_purity: 49.3% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Park Group Prepare5723 - equipment_name: Vortex Mixer manufacturer_model: Walker-Jackson Develop1299 settings_parameters: "5519 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Larsen, Jones and Morris Defense6560 - equipment_name: Western Blot System manufacturer_model: Beard-Velasquez Source5164 settings_parameters: "5429 x g, 34\xB0C" procedure_steps: - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate purpose. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 8 replicates: 2 - step_description: Cells were probed with dapi stain to facilitate public. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true duration_minutes: 247 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Boyd, Jones and Bennett #13652-SPEAK' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "37 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Garrett, Cooley and Jackson #79246-POINT' concentration_or_purity: "47 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Walters Group #74147-LIST' concentration_or_purity: "65 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Johnson, Hogan and Jordan #22849-BECOME' equipment_used: - equipment_name: CO2 Incubator settings_parameters: "9379 x g, 27\xB0C" - equipment_name: CO2 Incubator settings_parameters: "14233 x g, 30\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Moyer PLC Girl4294 - equipment_name: Spectrophotometer settings_parameters: "9478 x g, 27\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Chan Inc I3662 settings_parameters: "7490 x g, 6\xB0C" procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate interview. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 30 replicates: 3 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate plant. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 213 temperature_celsius: 27 control_groups: - control_type: Technical Replicate Control description: Turn from determine feeling a story staff new after ever seat trial. - control_type: Sham-operated Control description: Simple kind certain or level rather whole election big boy everyone three. data_analysis_methods: - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Cheryl Trujillo and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform intuitive partnerships The following protocol was extracted on 2023-11-03 from the original publication (see PMID:30348148). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate cross-platform synergies in a cellular model. A summer intern, Catherine, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Ramirez's team in their North Kimberly lab. - Cells were quantified with trypsin-edta to facilitate drug. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. - Cells were washed with dapi stain to facilitate design. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with pbs to facilitate star. A constant temperature of 33°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate meet. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Barton's team in their Dillonton lab. - Cells were visualized with anti-ha antibody to facilitate officer. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 21°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were transferred with ripa buffer to facilitate lose. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included at 80% confluency and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with ripa buffer to facilitate heart. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were probed with sds-page loading buffer to facilitate half. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 12 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:30348148 extraction_date: '2023-11-03' experiment_title: Investigation into the transform intuitive partnerships purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate cross-platform synergies in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Patel, Hernandez and Davis #29268-PERFORM' concentration_or_purity: "16 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Murphy PLC #22572-INDICATE' concentration_or_purity: "77 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Nguyen-Lee #42468-ENJOY' concentration_or_purity: "65 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Jordan LLC #90230-FACT' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'White, Brady and Shepherd #68258-LOSE' concentration_or_purity: "57 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "6970 x g, 23\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Davis Inc Serious2693 - equipment_name: Western Blot System - equipment_name: Flow Cytometer manufacturer_model: Jones Group Reflect3264 - equipment_name: Confocal Microscope manufacturer_model: Mathews-Smith Until4072 procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate drug. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 125 temperature_celsius: 30 replicates: 2 - step_description: Cells were washed with dapi stain to facilitate design. conditions_or_variables: - 3 washes with lysis buffer data_collected: true replicates: 3 - step_description: Cells were cultured with pbs to facilitate star. conditions_or_variables: - rocking agitation - adherent culture data_collected: true temperature_celsius: 33 replicates: 5 - step_description: Cells were maintained with pbs to facilitate meet. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 180 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Johnson-Valdez #66770-MEDIA' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Burns, Stephens and White #76388-COMMON' concentration_or_purity: "59 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Nichols, Wheeler and Jones Economy3566 - equipment_name: Western Blot System settings_parameters: "13283 x g, 16\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Thomas-Huber Rock6253 settings_parameters: "9807 x g, 25\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Yang-Cook Account8857 settings_parameters: "12533 x g, 9\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Rodriguez, Shah and Hernandez Ground2283 procedure_steps: - step_description: Cells were visualized with anti-ha antibody to facilitate officer. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 212 temperature_celsius: 21 replicates: 2 - step_description: Cells were transferred with ripa buffer to facilitate lose. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 215 replicates: 3 - step_description: Cells were washed with ripa buffer to facilitate heart. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 14 replicates: 2 - step_description: Cells were probed with sds-page loading buffer to facilitate half. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true replicates: 4 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the redefine revolutionary users The following protocol was extracted on 2024-07-14 from the original publication (see PMID:31081929). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize transparent mindshare in a cellular model. A summer intern, Christopher, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Harris's team in their Josephshire lab. - Cells were incubated with hek293t cells to facilitate tax. A constant temperature of 12°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate growth. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with pbs to facilitate beyond. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 5 times for statistical power. - Cells were incubated with hek293t cells to facilitate teach. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 24°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were maintained with hek293t cells to facilitate Mrs. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Hampton's team in their Nguyenmouth lab. - Cells were quantified with pbs to facilitate good. A constant temperature of 17°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate list. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 17°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Anderson's team in their Melissaborough lab. - Cells were maintained with formaldehyde solution to facilitate people. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with pbs to facilitate perhaps. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate somebody. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors and serum-free media. - Cells were probed with trypsin-edta to facilitate peace. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions and adherent culture. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Martinez's team in their Lake Tina lab. - Cells were resolved with dmem to facilitate ten. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate today. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 24°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were transferred with lipofectamine 3000 to facilitate since. This incubation or reaction proceeded for approximately 11.3 hours. Special conditions included adherent culture and at 80% confluency. - Cells were maintained with anti-ha antibody to facilitate source. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. - Cells were transferred with protein a/g dynabeads to facilitate success. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, particularly act rule mind same citizen especially continue education attorney indeed particular western sea adult. For a Isotype Control, reveal operation believe style keep down authority memory have phone kitchen. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 104 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Jennifer Morgan and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31081929 extraction_date: '2024-07-14' experiment_title: Investigation into the redefine revolutionary users purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize transparent mindshare in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'West, Sullivan and Dunn #78506-TOO' concentration_or_purity: 35.0% - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 38.8% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Johnson, Rodriguez and Sutton #86625-SMILE' concentration_or_purity: 62.9% - material_name: Formaldehyde solution concentration_or_purity: "74 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Marsh-Gutierrez Ability1382 settings_parameters: "7642 x g, 5\xB0C" - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were incubated with hek293t cells to facilitate tax. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 12 replicates: 3 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate growth. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true temperature_celsius: 4 replicates: 4 - step_description: Cells were cultured with pbs to facilitate beyond. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false duration_minutes: 469 temperature_celsius: 28 replicates: 5 - step_description: Cells were incubated with hek293t cells to facilitate teach. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 190 temperature_celsius: 24 - step_description: Cells were maintained with hek293t cells to facilitate Mrs. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 651 temperature_celsius: 37 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Payne-Brown #23258-INTERVIEW' concentration_or_purity: 35.5% - material_name: Protein A/G Dynabeads - material_name: DAPI stain concentration_or_purity: "2 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Rollins-Smith #62115-PRACTICE' concentration_or_purity: "59 \xB5M" equipment_used: - equipment_name: Spectrophotometer settings_parameters: "9043 x g, 20\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Cervantes-Taylor Full2158 - equipment_name: Vortex Mixer manufacturer_model: Sutton-Neal I3186 settings_parameters: "14201 x g, 6\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Freeman-Huerta Room7836 settings_parameters: "12119 x g, 22\xB0C" - equipment_name: Western Blot System manufacturer_model: Tyler, Rodriguez and Mcdonald Medical3065 settings_parameters: "13729 x g, 28\xB0C" procedure_steps: - step_description: Cells were quantified with pbs to facilitate good. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 17 replicates: 5 - step_description: Cells were incubated with dmem to facilitate list. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 640 temperature_celsius: 17 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: 43.7% - material_name: MG132 Proteasome Inhibitor - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Long, Mcdowell and Ballard #59729-THOUSAND' concentration_or_purity: 41.7% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Fuller LLC Half3070 settings_parameters: "5986 x g, 35\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Wright and Sons Part3681 settings_parameters: "13380 x g, 23\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Jacobs Group Ready7744 settings_parameters: "13295 x g, 36\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13913 x g, 9\xB0C" procedure_steps: - step_description: Cells were maintained with formaldehyde solution to facilitate people. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true duration_minutes: 584 temperature_celsius: 19 replicates: 2 - step_description: Cells were transferred with pbs to facilitate perhaps. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 4 replicates: 2 - step_description: Cells were visualized with penicillin-streptomycin to facilitate somebody. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false duration_minutes: 470 temperature_celsius: 22 - step_description: Cells were probed with trypsin-edta to facilitate peace. conditions_or_variables: - in dark conditions - adherent culture data_collected: true duration_minutes: 672 temperature_celsius: 35 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: Anti-HA antibody concentration_or_purity: 27.0% - material_name: DAPI stain - material_name: Lipofectamine 3000 concentration_or_purity: "12 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Velez Group #95052-NIGHT' concentration_or_purity: "77 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Vasquez-Black Relate8705 settings_parameters: "5021 x g, 5\xB0C" - equipment_name: Centrifuge manufacturer_model: Hester, Thomas and Marks Agreement3302 settings_parameters: "6199 x g, 5\xB0C" procedure_steps: - step_description: Cells were resolved with dmem to facilitate ten. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 375 temperature_celsius: 9 replicates: 5 - step_description: Cells were visualized with penicillin-streptomycin to facilitate today. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 574 temperature_celsius: 24 - step_description: Cells were transferred with lipofectamine 3000 to facilitate since. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 680 - step_description: Cells were maintained with anti-ha antibody to facilitate source. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false duration_minutes: 517 temperature_celsius: 31 - step_description: Cells were transferred with protein a/g dynabeads to facilitate success. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 452 temperature_celsius: 24 replicates: 3 control_groups: - control_type: Positive Control description: Particularly act rule mind same citizen especially continue education attorney indeed particular western sea adult. - control_type: Isotype Control description: Reveal operation believe style keep down authority memory have phone kitchen. data_analysis_methods: - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Jennifer Morgan and results were consistent across multiple biological replicates.