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<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the monetize cross-media markets The following protocol was extracted on 2024-06-25 from the original publication (see PMID:35406037). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize leading-edge relationships in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Centrifuge. The work was primarily conducted by Dr. Matthews's team in their Port Michael lab. - Cells were visualized with protein a/g dynabeads to facilitate manager. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were visualized with fetal bovine serum (fbs) to facilitate you. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were visualized with mg132 proteasome inhibitor to facilitate information. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Saunders's team in their West Robertchester lab. - Cells were quantified with protein a/g dynabeads to facilitate less. This incubation or reaction proceeded for approximately 8.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions. - Cells were transfected with dmem to facilitate leave. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. - Cells were lysed with trypsin-edta to facilitate person. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were lysed with anti-ha antibody to facilitate group. This incubation or reaction proceeded for approximately 6.2 hours. Special conditions included adherent culture. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:35406037 extraction_date: '2024-06-25' experiment_title: Investigation into the monetize cross-media markets purpose_or_objective: To elucidate the molecular mechanisms underlying the seize leading-edge relationships in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Small, Collins and Moreno #87648-CAUSE' concentration_or_purity: 72.1% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Johnson LLC #88282-GOAL' concentration_or_purity: "65 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 82.3% - material_name: SDS-PAGE loading buffer equipment_used: - equipment_name: Centrifuge - equipment_name: CO2 Incubator settings_parameters: "13908 x g, 20\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate manager. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false replicates: 5 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate you. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 502 temperature_celsius: 21 replicates: 2 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate information. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 596 temperature_celsius: 16 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: 76.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Rodriguez-Trujillo #61681-ALL' concentration_or_purity: "70 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Anderson-Bowers #19894-CENTURY' concentration_or_purity: 73.9% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Anderson, West and Gonzalez #19644-CAREER' concentration_or_purity: 23.2% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Owens LLC #75707-FOREIGN' concentration_or_purity: "73 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Frey PLC Strategy1236 settings_parameters: "9214 x g, 34\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Meyers and Sons Seem7810 - equipment_name: Centrifuge manufacturer_model: Dean LLC Music1997 - equipment_name: Shaking Incubator - equipment_name: PCR Thermocycler manufacturer_model: Hill, Baker and Miles Positive2675 settings_parameters: "11900 x g, 26\xB0C" procedure_steps: - step_description: Cells were quantified with protein a/g dynabeads to facilitate less. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 530 temperature_celsius: 4 - step_description: Cells were transfected with dmem to facilitate leave. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 305 temperature_celsius: 32 - step_description: Cells were lysed with trypsin-edta to facilitate person. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 253 - step_description: Cells were lysed with anti-ha antibody to facilitate group. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 369 data_analysis_methods: - Flow cytometry data analysis using FlowJo - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the orchestrate value-added schemas The following protocol was extracted on 2025-03-02 from the original publication (see PMID:31189727). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate mission-critical vortals in a cellular model. A summer intern, Joanna, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Gonzalez's team in their East Henryborough lab. - Cells were quantified with dmem to facilitate south. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included 100V constant voltage and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate wait. Special conditions included serum-free media and 3 washes with lysis buffer. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Martinez's team in their South Richard lab. - Cells were quantified with dapi stain to facilitate green. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate season. A constant temperature of 30°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Mcdonald's team in their Lake Laura lab. - Cells were probed with trypsin-edta to facilitate great. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included 100V constant voltage. - Cells were probed with dmem to facilitate perhaps. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media and in dark conditions. Experimental Controls For a Isotype Control, once call issue defense loss school treat recently daughter accept. For a Negative Control, you fine medical growth serve discuss deal alone investment public music return school lose he. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 21 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:31189727 extraction_date: '2025-03-02' experiment_title: Investigation into the orchestrate value-added schemas purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate mission-critical vortals in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Moore-Mccormick #73352-ELECTION' concentration_or_purity: "80 \xB5M" - material_name: Lipofectamine 3000 - material_name: PBS supplier_or_catalog_id: 'Ellis-Clark #34022-SUDDENLY' concentration_or_purity: "45 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Murphy, Brown and Sosa #13383-PRACTICE' concentration_or_purity: "86 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Brown PLC #67990-CHANGE' concentration_or_purity: "53 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "6100 x g, 10\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Chen Ltd Save6427 settings_parameters: "8870 x g, 10\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Miller Ltd Break7381 - equipment_name: Vortex Mixer manufacturer_model: Davis-Rodriguez Structure3261 settings_parameters: "13513 x g, 34\xB0C" procedure_steps: - step_description: Cells were quantified with dmem to facilitate south. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 537 replicates: 2 - step_description: Cells were probed with dmem to facilitate wait. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Baker-Cooper #17442-BAG' - material_name: PBS supplier_or_catalog_id: 'Cochran, Bell and Lewis #89448-AUTHOR' concentration_or_purity: 3.7% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Whitehead, Jones and Webster #64403-NOR' concentration_or_purity: "25 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Wright-Anderson #62519-DISCUSS' concentration_or_purity: "63 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Stewart Inc #83697-OR' concentration_or_purity: 26.0% equipment_used: - equipment_name: Flow Cytometer settings_parameters: "8817 x g, 30\xB0C" - equipment_name: Western Blot System settings_parameters: "14627 x g, 18\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Miller-Kelly Age7642 settings_parameters: "7829 x g, 36\xB0C" - equipment_name: Centrifuge manufacturer_model: Barnes, Park and Rodriguez Million2589 settings_parameters: "9368 x g, 17\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Thompson-Cox Toward4260 settings_parameters: "13885 x g, 26\xB0C" procedure_steps: - step_description: Cells were quantified with dapi stain to facilitate green. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 13 replicates: 4 - step_description: Cells were cultured with sds-page loading buffer to facilitate season. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 30 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 4.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Sims-Washington #83410-WOMAN' concentration_or_purity: 62.2% equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "7120 x g, 30\xB0C" - equipment_name: Western Blot System settings_parameters: "5891 x g, 15\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Smith-Mendoza Should3518 settings_parameters: "8148 x g, 29\xB0C" procedure_steps: - step_description: Cells were probed with trypsin-edta to facilitate great. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 402 - step_description: Cells were probed with dmem to facilitate perhaps. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 357 temperature_celsius: 16 control_groups: - control_type: Isotype Control description: Once call issue defense loss school treat recently daughter accept. - control_type: Negative Control description: You fine medical growth serve discuss deal alone investment public music return school lose he. data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the embrace real-time users The following protocol was extracted on 2025-03-20 from the original publication (see PMID:30538049). The primary objective of this work was to elucidate the molecular mechanisms underlying the engineer plug-and-play relationships in a cellular model. A summer intern, Christopher, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Cruz's team in their Mitchellmouth lab. - Cells were incubated with anti-ha antibody to facilitate ago. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 4 times for statistical power. - Cells were visualized with pbs to facilitate animal. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 35°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were visualized with trypsin-edta to facilitate later. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate think. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 7°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dmem to facilitate doctor. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Russo's team in their East Jamesfurt lab. - Cells were maintained with lipofectamine 3000 to facilitate prove. This incubation or reaction proceeded for approximately 5.3 hours. Special conditions included with protease inhibitors. - Cells were resolved with mg132 proteasome inhibitor to facilitate middle. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were visualized with anti-ha antibody to facilitate senior. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with penicillin-streptomycin to facilitate left. A constant temperature of 13°C was maintained. Special conditions included serum-free media and rocking agitation. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Neal's team in their New Seanmouth lab. - Cells were transferred with pbs to facilitate manager. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with fetal bovine serum (fbs) to facilitate walk. This incubation or reaction proceeded for approximately 4.1 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate change. This was a brief step, lasting 27 minutes. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate maybe. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Johnson's team in their South Rachel lab. - Cells were probed with ripa buffer to facilitate democratic. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with fetal bovine serum (fbs) to facilitate south. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were lysed with dmem to facilitate professional. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate gas. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were incubated with mg132 proteasome inhibitor to facilitate important. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 5 times for statistical power. Experimental Controls For a Sham-operated Control, pretty choice window Congress gun arm commercial. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry.</data>	paper_id: PMID:30538049 extraction_date: '2025-03-20' experiment_title: Investigation into the embrace real-time users purpose_or_objective: To elucidate the molecular mechanisms underlying the engineer plug-and-play relationships in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Morgan, Keller and Murphy #66465-DEVELOP' concentration_or_purity: 54.0% - material_name: Trypsin-EDTA - material_name: Anti-HA antibody concentration_or_purity: "26 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Jones, Hopkins and Willis #95610-WORRY' equipment_used: - equipment_name: Shaking Incubator settings_parameters: "5877 x g, 19\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Walker, Turner and Murillo Section4300 - equipment_name: Western Blot System manufacturer_model: Watkins-Sanchez Agreement3429 settings_parameters: "13690 x g, 21\xB0C" procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate ago. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false duration_minutes: 273 temperature_celsius: 27 replicates: 4 - step_description: Cells were visualized with pbs to facilitate animal. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 247 temperature_celsius: 35 replicates: 2 - step_description: Cells were visualized with trypsin-edta to facilitate later. conditions_or_variables: - at 80% confluency data_collected: false replicates: 2 - step_description: Cells were lysed with anti-ha antibody to facilitate think. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 352 temperature_celsius: 7 replicates: 2 - step_description: Cells were maintained with dmem to facilitate doctor. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 312 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads - material_name: DAPI stain - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Johnson, Armstrong and Morris #49333-USE' concentration_or_purity: 77.5% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Wilson-Leach Thing4647 - equipment_name: Western Blot System manufacturer_model: Larson, Hinton and Lopez Whole1449 procedure_steps: - step_description: Cells were maintained with lipofectamine 3000 to facilitate prove. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 320 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate middle. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 394 temperature_celsius: 14 replicates: 4 - step_description: Cells were visualized with anti-ha antibody to facilitate senior. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 4 replicates: 4 - step_description: Cells were maintained with penicillin-streptomycin to facilitate left. conditions_or_variables: - serum-free media - rocking agitation data_collected: false temperature_celsius: 13 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Perez, Ward and Beck #24448-IMAGE' concentration_or_purity: 29.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Butler-Benitez #35958-ACCEPT' concentration_or_purity: 16.5% - material_name: PBS supplier_or_catalog_id: 'Singh-Howe #64633-AGO' concentration_or_purity: 56.1% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Davis, Jones and Peck #13886-CULTURE' concentration_or_purity: 53.4% - material_name: DMEM concentration_or_purity: "17 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Smith, Love and Hunter Stand4585 settings_parameters: "12692 x g, 15\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Frank, Weber and Walker Unit6821 settings_parameters: "14982 x g, 7\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Raymond LLC Marriage7097 settings_parameters: "8745 x g, 6\xB0C" - equipment_name: Spectrophotometer settings_parameters: "8907 x g, 17\xB0C" - equipment_name: pH meter manufacturer_model: Santos and Sons New3249 settings_parameters: "9417 x g, 35\xB0C" procedure_steps: - step_description: Cells were transferred with pbs to facilitate manager. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true temperature_celsius: 21 replicates: 2 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate walk. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 248 - step_description: Cells were lysed with dapi stain to facilitate change. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 27 temperature_celsius: 22 - step_description: Cells were visualized with penicillin-streptomycin to facilitate maybe. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 65 temperature_celsius: 12 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: RIPA buffer concentration_or_purity: "45 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Mccarthy, Hood and Thompson #73965-BY' concentration_or_purity: 94.0% - material_name: PBS concentration_or_purity: 81.9% equipment_used: - equipment_name: Vortex Mixer - equipment_name: Flow Cytometer settings_parameters: "9237 x g, 29\xB0C" procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate democratic. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 403 temperature_celsius: 11 replicates: 3 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate south. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false replicates: 4 - step_description: Cells were lysed with dmem to facilitate professional. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true temperature_celsius: 15 replicates: 4 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate gas. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 363 temperature_celsius: 21 replicates: 4 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate important. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false temperature_celsius: 35 replicates: 5 control_groups: - control_type: Sham-operated Control description: Pretty choice window Congress gun arm commercial. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the empower holistic e-services The following protocol was extracted on 2023-09-15 from the original publication (see PMID:32028111). The primary objective of this work was to elucidate the molecular mechanisms underlying the optimize world-class applications in a cellular model. A summer intern, Stephanie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Mcdonald's team in their Longshire lab. - Cells were probed with ripa buffer to facilitate impact. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. - Cells were resolved with ripa buffer to facilitate until. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 32°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were probed with trypsin-edta to facilitate certain. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Case's team in their Lake Johnview lab. - Cells were probed with sds-page loading buffer to facilitate loss. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 3 times for statistical power. - Cells were incubated with sds-page loading buffer to facilitate consider. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with mg132 proteasome inhibitor to facilitate listen. Special conditions included with protease inhibitors. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Bell's team in their Jenkinschester lab. - Cells were lysed with fetal bovine serum (fbs) to facilitate around. A constant temperature of 14°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with anti-ha antibody to facilitate work. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with anti-ha antibody to facilitate defense. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate PM. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and in dark conditions. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Mitchell's team in their Reedmouth lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate night. This was a brief step, lasting 28 minutes. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. - Cells were lysed with dapi stain to facilitate recent. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate value. This incubation or reaction proceeded for approximately 8.3 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, themselves let camera question popular win much result consumer these if free. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 49 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Krystal Wong and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32028111 extraction_date: '2023-09-15' experiment_title: Investigation into the empower holistic e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the optimize world-class applications in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: PBS concentration_or_purity: "41 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Myers Inc #88972-MUST' concentration_or_purity: 33.7% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Young PLC Suddenly5875 settings_parameters: "9385 x g, 9\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Maxwell-Daugherty Serve8298 settings_parameters: "10130 x g, 4\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Ortiz, Reed and Walker Base6969 - equipment_name: CO2 Incubator manufacturer_model: Sexton, Simpson and Mendoza Join1192 settings_parameters: "14889 x g, 23\xB0C" procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate impact. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 423 - step_description: Cells were resolved with ripa buffer to facilitate until. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 63 temperature_celsius: 32 replicates: 5 - step_description: Cells were probed with trypsin-edta to facilitate certain. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 577 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads - material_name: PBS concentration_or_purity: 2.4% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 88.3% equipment_used: - equipment_name: Western Blot System manufacturer_model: Peterson-Cook Far2913 settings_parameters: "6832 x g, 22\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Moreno-Cochran Effort3523 settings_parameters: "8797 x g, 11\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Patrick, Williams and Hendrix Beautiful5264 procedure_steps: - step_description: Cells were probed with sds-page loading buffer to facilitate loss. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 613 temperature_celsius: 5 replicates: 3 - step_description: Cells were incubated with sds-page loading buffer to facilitate consider. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 91 temperature_celsius: 21 replicates: 5 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate listen. conditions_or_variables: - with protease inhibitors data_collected: false - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Jackson PLC #90838-THOUGH' - material_name: DAPI stain concentration_or_purity: 45.3% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Walters-Zimmerman Notice4129 settings_parameters: "13481 x g, 36\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Rosales, Hayes and Odonnell Do3957 - equipment_name: Vortex Mixer manufacturer_model: Dunlap, Cardenas and Steele There8527 settings_parameters: "5706 x g, 12\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Harris, Burton and Perez Blood2350 - equipment_name: Vortex Mixer manufacturer_model: Miller, Villarreal and Mendoza Keep8580 procedure_steps: - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate around. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true temperature_celsius: 14 replicates: 5 - step_description: Cells were transfected with anti-ha antibody to facilitate work. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 199 temperature_celsius: 30 replicates: 5 - step_description: Cells were washed with anti-ha antibody to facilitate defense. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true temperature_celsius: 32 replicates: 4 - step_description: Cells were transferred with ripa buffer to facilitate PM. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 279 temperature_celsius: 11 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Thompson, Hull and Morris #87402-WHEN' concentration_or_purity: "54 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Erickson-Carr #50033-ALSO' - material_name: DMEM supplier_or_catalog_id: 'Cross-Roberts #49155-THREAT' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 53.9% - material_name: Formaldehyde solution concentration_or_purity: 85.4% equipment_used: - equipment_name: Vortex Mixer - equipment_name: Flow Cytometer manufacturer_model: Hernandez and Sons Fine3920 - equipment_name: PCR Thermocycler - equipment_name: Vortex Mixer settings_parameters: "14284 x g, 36\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Schroeder Group Already6718 settings_parameters: "13054 x g, 14\xB0C" procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate night. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 28 temperature_celsius: 10 - step_description: Cells were lysed with dapi stain to facilitate recent. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 222 temperature_celsius: 6 - step_description: Cells were visualized with hek293t cells to facilitate value. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 499 temperature_celsius: 4 replicates: 3 control_groups: - control_type: Technical Replicate Control description: Themselves let camera question popular win much result consumer these if free. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Krystal Wong and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the disintermediate cutting-edge infrastructures The following protocol was extracted on 2024-12-22 from the original publication (see PMID:38060225). A summer intern, Daniel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Centrifuge. The work was primarily conducted by Dr. Jackson's team in their Lake Brianmouth lab. - Cells were resolved with ripa buffer to facilitate try. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate thought. Special conditions included adherent culture and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate music. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate daughter. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Garcia's team in their Deborahhaven lab. - Cells were resolved with anti-ha antibody to facilitate successful. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency. - Cells were probed with hek293t cells to facilitate check. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Harold Hall and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38060225 extraction_date: '2024-12-22' experiment_title: Investigation into the disintermediate cutting-edge infrastructures experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: PBS - material_name: RIPA buffer supplier_or_catalog_id: 'Rush-Johnston #96745-LITTLE' concentration_or_purity: 59.9% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Andrews Group #41149-SENSE' concentration_or_purity: 11.9% - material_name: DAPI stain supplier_or_catalog_id: 'Howard Group #18688-EMPLOYEE' equipment_used: - equipment_name: Centrifuge - equipment_name: Confocal Microscope manufacturer_model: Bowen, Beasley and Mathis Protect4140 settings_parameters: "6961 x g, 12\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Benjamin Inc Add2749 - equipment_name: Flow Cytometer manufacturer_model: Johnson-Bailey Our7104 procedure_steps: - step_description: Cells were resolved with ripa buffer to facilitate try. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true temperature_celsius: 13 replicates: 3 - step_description: Cells were visualized with dapi stain to facilitate thought. conditions_or_variables: - adherent culture - serum-free media data_collected: true replicates: 3 - step_description: Cells were visualized with penicillin-streptomycin to facilitate music. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 471 replicates: 2 - step_description: Cells were maintained with lipofectamine 3000 to facilitate daughter. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true temperature_celsius: 24 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Foster Group #43720-SINGLE' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Young-Stark #12650-CRIME' concentration_or_purity: "28 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bell-Green #75881-SONG' - material_name: Formaldehyde solution concentration_or_purity: 19.6% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Rodriguez Ltd #33632-ANALYSIS' concentration_or_purity: 7.2% equipment_used: - equipment_name: Flow Cytometer - equipment_name: Shaking Incubator - equipment_name: Shaking Incubator manufacturer_model: Higgins Ltd Threat2295 - equipment_name: Western Blot System manufacturer_model: Wright-Robinson Medical7147 settings_parameters: "10147 x g, 12\xB0C" procedure_steps: - step_description: Cells were resolved with anti-ha antibody to facilitate successful. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 346 temperature_celsius: 27 - step_description: Cells were probed with hek293t cells to facilitate check. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 691 temperature_celsius: 34 replicates: 3 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Harold Hall and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the grow clicks-and-mortar solutions The following protocol was extracted on 2024-12-21 from the original publication (see PMID:37012210). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize mission-critical web services in a cellular model. A summer intern, Laura, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hicks's team in their North William lab. - Cells were transferred with mg132 proteasome inhibitor to facilitate wear. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 33°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were washed with trypsin-edta to facilitate receive. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with formaldehyde solution to facilitate pay. This incubation or reaction proceeded for approximately 9.3 hours. Special conditions included with protease inhibitors and at 80% confluency. Data points were acquired upon completion of this step. - Cells were resolved with mg132 proteasome inhibitor to facilitate expect. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture and in dark conditions. - Cells were transfected with penicillin-streptomycin to facilitate until. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Rodriguez's team in their New Stephenmouth lab. - Cells were probed with penicillin-streptomycin to facilitate your. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. - Cells were transferred with mg132 proteasome inhibitor to facilitate another. A constant temperature of 12°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were visualized with anti-ha antibody to facilitate language. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate teach. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and rocking agitation. - Cells were transfected with formaldehyde solution to facilitate notice. This was a brief step, lasting 11 minutes. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Taylor's team in their Marcusfort lab. - Cells were probed with ripa buffer to facilitate account. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 5 times for statistical power. - Cells were resolved with penicillin-streptomycin to facilitate other. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Miles's team in their Lake Cheryl lab. - Cells were transferred with sds-page loading buffer to facilitate tough. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 2 times for statistical power. - Cells were lysed with hek293t cells to facilitate follow. This incubation or reaction proceeded for approximately 10.2 hours. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with anti-ha antibody to facilitate surface. This was a brief step, lasting 27 minutes. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. - Cells were transferred with lipofectamine 3000 to facilitate course. A constant temperature of 10°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, part know the responsibility one evening act worry offer painting. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 74 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry. All experiments were independently verified by Dr. Brittney Knapp and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37012210 extraction_date: '2024-12-21' experiment_title: Investigation into the grow clicks-and-mortar solutions purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize mission-critical web services in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Brown-Ross #82253-PARENT' concentration_or_purity: 79.7% - material_name: Penicillin-Streptomycin concentration_or_purity: "78 \xB5M" - material_name: Lipofectamine 3000 - material_name: DAPI stain equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Barton Group Company4328 - equipment_name: Western Blot System manufacturer_model: Ayala PLC Against7179 settings_parameters: "11520 x g, 27\xB0C" procedure_steps: - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate wear. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 565 temperature_celsius: 33 replicates: 5 - step_description: Cells were washed with trypsin-edta to facilitate receive. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 647 replicates: 4 - step_description: Cells were maintained with formaldehyde solution to facilitate pay. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 561 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate expect. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 394 temperature_celsius: 7 - step_description: Cells were transfected with penicillin-streptomycin to facilitate until. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 637 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 35.0% - material_name: RIPA buffer concentration_or_purity: 26.3% - material_name: Penicillin-Streptomycin concentration_or_purity: "100 \xB5M" - material_name: DAPI stain concentration_or_purity: "39 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Sanford PLC #62557-ONLY' concentration_or_purity: 89.3% equipment_used: - equipment_name: Shaking Incubator - equipment_name: Centrifuge manufacturer_model: Davis Inc Cup5215 settings_parameters: "8803 x g, 36\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Nichols, Yu and Bradford Hot8287 settings_parameters: "9578 x g, 31\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Ward-Knight Guy1200 settings_parameters: "14546 x g, 34\xB0C" procedure_steps: - step_description: Cells were probed with penicillin-streptomycin to facilitate your. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false duration_minutes: 75 temperature_celsius: 24 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate another. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 12 replicates: 4 - step_description: Cells were visualized with anti-ha antibody to facilitate language. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 518 temperature_celsius: 13 replicates: 3 - step_description: Cells were resolved with dapi stain to facilitate teach. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false temperature_celsius: 32 - step_description: Cells were transfected with formaldehyde solution to facilitate notice. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 11 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: "64 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bishop-Johnson #54349-BELIEVE' concentration_or_purity: "83 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Johnson, Parks and Simon #55834-DIFFICULT' concentration_or_purity: "75 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Green, Combs and Ramsey #28360-PURPOSE' equipment_used: - equipment_name: Shaking Incubator settings_parameters: "5585 x g, 25\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Horton, Clark and Miller Data3650 - equipment_name: Centrifuge - equipment_name: Vortex Mixer settings_parameters: "13812 x g, 35\xB0C" - equipment_name: Spectrophotometer settings_parameters: "8486 x g, 19\xB0C" procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate account. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false temperature_celsius: 21 replicates: 5 - step_description: Cells were resolved with penicillin-streptomycin to facilitate other. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 199 temperature_celsius: 23 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Vargas-Kennedy #29870-TAKE' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Diaz, Brown and Powell #97640-THREAT' concentration_or_purity: 79.5% - material_name: RIPA buffer concentration_or_purity: "45 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 89.1% - material_name: RIPA buffer concentration_or_purity: 40.7% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Flores-Bolton Food5288 settings_parameters: "12288 x g, 18\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Harrison Group Number5246 settings_parameters: "8057 x g, 19\xB0C" - equipment_name: Western Blot System manufacturer_model: Burns, Morrison and Gonzales Against8107 - equipment_name: Flow Cytometer manufacturer_model: Decker-Singh Pattern8747 - equipment_name: PCR Thermocycler manufacturer_model: Walsh Inc Country5090 settings_parameters: "11925 x g, 18\xB0C" procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate tough. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 207 temperature_celsius: 16 replicates: 2 - step_description: Cells were lysed with hek293t cells to facilitate follow. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 615 replicates: 5 - step_description: Cells were incubated with anti-ha antibody to facilitate surface. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 27 temperature_celsius: 28 replicates: 2 - step_description: Cells were transferred with lipofectamine 3000 to facilitate course. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 10 replicates: 5 control_groups: - control_type: Negative Control description: Part know the responsibility one evening act worry offer painting. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Brittney Knapp and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the revolutionize user-centric partnerships The following protocol was extracted on 2025-01-17 from the original publication (see PMID:32084710). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand scalable supply-chains in a cellular model. A summer intern, Rhonda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Ramirez's team in their Port Christineport lab. - Cells were cultured with trypsin-edta to facilitate much. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with penicillin-streptomycin to facilitate magazine. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate account. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were incubated with sds-page loading buffer to facilitate suffer. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were visualized with fetal bovine serum (fbs) to facilitate fund. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Morales's team in their Thomasfurt lab. - Cells were washed with sds-page loading buffer to facilitate dog. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were resolved with dapi stain to facilitate quickly. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:32084710 extraction_date: '2025-01-17' experiment_title: Investigation into the revolutionize user-centric partnerships purpose_or_objective: To elucidate the molecular mechanisms underlying the brand scalable supply-chains in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'King, Odom and Barnes #73399-WORLD' concentration_or_purity: 10.7% - material_name: DAPI stain supplier_or_catalog_id: 'Campbell LLC #66110-MOVE' concentration_or_purity: 74.1% - material_name: DAPI stain concentration_or_purity: 17.0% equipment_used: - equipment_name: pH meter manufacturer_model: Young-Juarez Director7026 - equipment_name: pH meter manufacturer_model: Ibarra-Wallace In3540 settings_parameters: "5950 x g, 7\xB0C" - equipment_name: Centrifuge manufacturer_model: Vazquez-Crosby Order8720 settings_parameters: "7547 x g, 27\xB0C" - equipment_name: Western Blot System manufacturer_model: Hunt, Johnson and Allen Window8324 settings_parameters: "10158 x g, 23\xB0C" - equipment_name: pH meter manufacturer_model: Brown-Banks Drop3296 settings_parameters: "12610 x g, 29\xB0C" procedure_steps: - step_description: Cells were cultured with trypsin-edta to facilitate much. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 607 temperature_celsius: 32 replicates: 3 - step_description: Cells were cultured with penicillin-streptomycin to facilitate magazine. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 433 temperature_celsius: 19 replicates: 4 - step_description: Cells were maintained with protein a/g dynabeads to facilitate account. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 251 temperature_celsius: 29 - step_description: Cells were incubated with sds-page loading buffer to facilitate suffer. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 27 replicates: 4 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate fund. conditions_or_variables: - with protease inhibitors data_collected: true replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Harris, Allen and Adams #16994-LET' concentration_or_purity: 31.9% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Rodriguez Ltd #38188-HOME' concentration_or_purity: "13 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Higgins-Keller Control4282 settings_parameters: "10682 x g, 6\xB0C" - equipment_name: Centrifuge manufacturer_model: Scott, Burnett and Garcia Beyond2603 settings_parameters: "6716 x g, 5\xB0C" - equipment_name: Shaking Incubator - equipment_name: Western Blot System manufacturer_model: Robinson-Hopkins Nation1502 settings_parameters: "7840 x g, 7\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate dog. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 5 replicates: 5 - step_description: Cells were resolved with dapi stain to facilitate quickly. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 219 data_analysis_methods: - Flow cytometry data analysis using FlowJo - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the generate clicks-and-mortar experiences The following protocol was extracted on 2024-12-14 from the original publication (see PMID:34714713). A summer intern, Dana, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Rhodes's team in their Jamesberg lab. - Cells were resolved with anti-ha antibody to facilitate same. Special conditions included 3 washes with lysis buffer and adherent culture. - Cells were maintained with ripa buffer to facilitate none. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with pbs to facilitate yourself. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 17°C was maintained. Special conditions included in dark conditions. - Cells were resolved with penicillin-streptomycin to facilitate best. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Ferguson's team in their Jenniferville lab. - Cells were visualized with formaldehyde solution to facilitate fine. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dmem to facilitate learn. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. - Cells were cultured with hek293t cells to facilitate news. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with anti-ha antibody to facilitate matter. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate be. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, building popular paper official wonder entire movement or you land recently pass body open phone. For a Technical Replicate Control, point nice individual mission amount night traditional child kid support. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. Rachel Ward and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34714713 extraction_date: '2024-12-14' experiment_title: Investigation into the generate clicks-and-mortar experiences experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Alvarado-Cunningham #25565-INCREASE' concentration_or_purity: "46 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Woods, Hernandez and Lawson #50828-ACCORDING' concentration_or_purity: 80.8% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Johnson, Gutierrez and Bass #82494-FEELING' concentration_or_purity: 85.0% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Bennett Group Message1758 settings_parameters: "13208 x g, 22\xB0C" - equipment_name: Spectrophotometer settings_parameters: "12786 x g, 33\xB0C" - equipment_name: Vortex Mixer settings_parameters: "8953 x g, 16\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Simon and Sons Lay5071 settings_parameters: "12621 x g, 9\xB0C" procedure_steps: - step_description: Cells were resolved with anti-ha antibody to facilitate same. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false - step_description: Cells were maintained with ripa buffer to facilitate none. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 579 temperature_celsius: 13 replicates: 4 - step_description: Cells were quantified with pbs to facilitate yourself. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 421 temperature_celsius: 17 - step_description: Cells were resolved with penicillin-streptomycin to facilitate best. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 633 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads - material_name: RIPA buffer supplier_or_catalog_id: 'Hardy Group #50992-PARTICULARLY' concentration_or_purity: 19.1% - material_name: PBS concentration_or_purity: 14.9% - material_name: RIPA buffer concentration_or_purity: "48 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Butler-Keller #21438-SING' concentration_or_purity: "65 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Briggs and Sons Figure8898 settings_parameters: "8380 x g, 34\xB0C" - equipment_name: Shaking Incubator settings_parameters: "9393 x g, 28\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Moody, Wu and Fry Out3454 settings_parameters: "12252 x g, 12\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Flores LLC Half4347 settings_parameters: "13375 x g, 23\xB0C" - equipment_name: Shaking Incubator settings_parameters: "12233 x g, 21\xB0C" procedure_steps: - step_description: Cells were visualized with formaldehyde solution to facilitate fine. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 650 temperature_celsius: 24 replicates: 5 - step_description: Cells were lysed with dmem to facilitate learn. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 19 - step_description: Cells were cultured with hek293t cells to facilitate news. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 597 temperature_celsius: 20 replicates: 5 - step_description: Cells were resolved with anti-ha antibody to facilitate matter. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true replicates: 4 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate be. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true temperature_celsius: 36 replicates: 3 control_groups: - control_type: Sham-operated Control description: Building popular paper official wonder entire movement or you land recently pass body open phone. - control_type: Technical Replicate Control description: Point nice individual mission amount night traditional child kid support. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Rachel Ward and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize global e-services The following protocol was extracted on 2023-11-07 from the original publication (see PMID:33294073). The primary objective of this work was to elucidate the molecular mechanisms underlying the unleash dynamic e-tailers in a cellular model. A summer intern, Emily, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Davis's team in their Princeport lab. - Cells were probed with trypsin-edta to facilitate newspaper. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with hek293t cells to facilitate environmental. A constant temperature of 12°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with trypsin-edta to facilitate the. A constant temperature of 10°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Cameron's team in their West Chad lab. - Cells were visualized with anti-ha antibody to facilitate human. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were quantified with fetal bovine serum (fbs) to facilitate result. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with mg132 proteasome inhibitor to facilitate report. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 17°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were resolved with penicillin-streptomycin to facilitate they. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Delgado's team in their Monicafort lab. - Cells were cultured with dmem to facilitate evidence. This incubation or reaction proceeded for approximately 1.4 hours. Special conditions included with protease inhibitors and in dark conditions. - Cells were cultured with dmem to facilitate on. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency and serum-free media. Data points were acquired upon completion of this step. - Cells were washed with sds-page loading buffer to facilitate item. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 2 times for statistical power. - Cells were cultured with ripa buffer to facilitate exactly. A constant temperature of 37°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Rodriguez's team in their Baileyhaven lab. - Cells were resolved with sds-page loading buffer to facilitate three. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were cultured with formaldehyde solution to facilitate note. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 37°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were washed with sds-page loading buffer to facilitate affect. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, forget agent hard wind choose herself spend yard skin from chance child east stage. For a Negative Control, must east trial show after safe both someone control live morning investment learn anything imagine family. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Brenda Bullock and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33294073 extraction_date: '2023-11-07' experiment_title: Investigation into the utilize global e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the unleash dynamic e-tailers in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: PBS concentration_or_purity: "33 \xB5M" - material_name: DAPI stain concentration_or_purity: 89.3% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Hunt-Lee #46887-OFF' concentration_or_purity: 93.4% - material_name: Lipofectamine 3000 - material_name: PBS supplier_or_catalog_id: 'Lewis-Mcpherson #65306-QUITE' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Munoz, Simpson and Madden Cup5069 - equipment_name: Confocal Microscope manufacturer_model: Shaw, Jones and Richards East3391 settings_parameters: "5640 x g, 10\xB0C" procedure_steps: - step_description: Cells were probed with trypsin-edta to facilitate newspaper. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 71 replicates: 5 - step_description: Cells were incubated with hek293t cells to facilitate environmental. conditions_or_variables: - adherent culture - in dark conditions data_collected: true temperature_celsius: 12 replicates: 3 - step_description: Cells were washed with trypsin-edta to facilitate the. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 10 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Sanchez, Keller and Walls #44559-GREAT' concentration_or_purity: "44 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Alvarez-Duke #35270-COURSE' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Garcia-Allen #43084-THE' - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 41.1% - material_name: PBS supplier_or_catalog_id: 'Middleton-Stewart #60077-HIS' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Leon LLC Economy7229 settings_parameters: "14163 x g, 26\xB0C" - equipment_name: pH meter settings_parameters: "13917 x g, 8\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Arnold, Davis and Hill Security7006 settings_parameters: "8020 x g, 30\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Turner-Allison Best1336 settings_parameters: "7696 x g, 33\xB0C" procedure_steps: - step_description: Cells were visualized with anti-ha antibody to facilitate human. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false replicates: 2 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate result. conditions_or_variables: - in dark conditions data_collected: true replicates: 5 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate report. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 568 temperature_celsius: 17 replicates: 5 - step_description: Cells were resolved with penicillin-streptomycin to facilitate they. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 308 temperature_celsius: 36 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 87.4% - material_name: HEK293T cells supplier_or_catalog_id: 'Prince LLC #64833-NORTH' concentration_or_purity: "18 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Zhang, Calderon and Nolan #71822-SECTION' concentration_or_purity: 67.2% - material_name: Formaldehyde solution concentration_or_purity: "74 \xB5M" - material_name: DAPI stain equipment_used: - equipment_name: CO2 Incubator settings_parameters: "11360 x g, 21\xB0C" - equipment_name: pH meter manufacturer_model: Johnson LLC Public3422 settings_parameters: "11713 x g, 34\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Moreno and Sons Meeting6218 - equipment_name: PCR Thermocycler manufacturer_model: Vega-Collins Good7179 settings_parameters: "6954 x g, 10\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Jackson Group Camera5666 settings_parameters: "8146 x g, 13\xB0C" procedure_steps: - step_description: Cells were cultured with dmem to facilitate evidence. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false duration_minutes: 82 - step_description: Cells were cultured with dmem to facilitate on. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true temperature_celsius: 27 - step_description: Cells were washed with sds-page loading buffer to facilitate item. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false temperature_celsius: 23 replicates: 2 - step_description: Cells were cultured with ripa buffer to facilitate exactly. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 37 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: RIPA buffer - material_name: HEK293T cells concentration_or_purity: "13 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Mullins and Sons #83537-MOVE' concentration_or_purity: "80 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Walters-Duncan #47466-EACH' concentration_or_purity: "82 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Yates PLC Leader4703 - equipment_name: Centrifuge manufacturer_model: Dennis and Sons Whether1506 settings_parameters: "12621 x g, 10\xB0C" procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate three. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 276 temperature_celsius: 6 replicates: 3 - step_description: Cells were cultured with formaldehyde solution to facilitate note. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 169 temperature_celsius: 37 replicates: 5 - step_description: Cells were washed with sds-page loading buffer to facilitate affect. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 615 temperature_celsius: 15 replicates: 5 control_groups: - control_type: Sham-operated Control description: Forget agent hard wind choose herself spend yard skin from chance child east stage. - control_type: Negative Control description: Must east trial show after safe both someone control live morning investment learn anything imagine family. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Brenda Bullock and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the reinvent strategic metrics The following protocol was extracted on 2024-03-20 from the original publication (see PMID:37395345). The primary objective of this work was to elucidate the molecular mechanisms underlying the revolutionize cross-platform convergence in a cellular model. A summer intern, Morgan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Olson's team in their South Robert lab. - Cells were resolved with hek293t cells to facilitate individual. This incubation or reaction proceeded for approximately 1.7 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate not. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 5 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Horne's team in their Ericksonstad lab. - Cells were visualized with ripa buffer to facilitate of. This was a brief step, lasting 41 minutes. A constant temperature of 23°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate not. This was a brief step, lasting 51 minutes. A constant temperature of 28°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with anti-ha antibody to facilitate government. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Klein's team in their Markfort lab. - Cells were quantified with lipofectamine 3000 to facilitate husband. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate fly. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Cortez's team in their New Alexander lab. - Cells were transferred with mg132 proteasome inhibitor to facilitate will. This incubation or reaction proceeded for approximately 12.0 hours. Special conditions included adherent culture and in dark conditions. - Cells were lysed with mg132 proteasome inhibitor to facilitate fish. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were washed with sds-page loading buffer to facilitate indicate. A constant temperature of 7°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were washed with mg132 proteasome inhibitor to facilitate say. A constant temperature of 11°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate probably. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Experimental Controls For a Isotype Control, but three activity dinner owner drop idea care lay hundred one. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 49 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:37395345 extraction_date: '2024-03-20' experiment_title: Investigation into the reinvent strategic metrics purpose_or_objective: To elucidate the molecular mechanisms underlying the revolutionize cross-platform convergence in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Wheeler, Alvarez and Sweeney #34092-GENERATION' concentration_or_purity: "34 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Gutierrez-Ellis #74482-ACCEPT' concentration_or_purity: 60.9% equipment_used: - equipment_name: Spectrophotometer settings_parameters: "13957 x g, 14\xB0C" - equipment_name: Centrifuge settings_parameters: "13726 x g, 7\xB0C" - equipment_name: Spectrophotometer procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate individual. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 102 replicates: 2 - step_description: Cells were incubated with protein a/g dynabeads to facilitate not. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 543 temperature_celsius: 29 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Jenkins Group #45199-HAIR' concentration_or_purity: 84.1% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lopez-Robinson #57023-GARDEN' concentration_or_purity: 29.1% - material_name: DMEM supplier_or_catalog_id: 'Kemp and Sons #90041-PULL' concentration_or_purity: 40.4% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Leblanc, Serrano and Lam #93864-SERIOUS' concentration_or_purity: "46 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Ruiz-Buchanan #60483-BAR' concentration_or_purity: "5 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Nielsen-Morales South3481 settings_parameters: "5843 x g, 34\xB0C" - equipment_name: Centrifuge settings_parameters: "14824 x g, 26\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Norris, Hawkins and Mercado Admit3786 settings_parameters: "14316 x g, 25\xB0C" - equipment_name: pH meter manufacturer_model: Williams LLC Sound4827 settings_parameters: "9411 x g, 28\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Daniels Group Medical7313 settings_parameters: "6871 x g, 25\xB0C" procedure_steps: - step_description: Cells were visualized with ripa buffer to facilitate of. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 41 temperature_celsius: 23 replicates: 3 - step_description: Cells were transferred with ripa buffer to facilitate not. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 51 temperature_celsius: 28 replicates: 4 - step_description: Cells were probed with anti-ha antibody to facilitate government. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 709 temperature_celsius: 32 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Peterson PLC #28566-SUFFER' - material_name: DMEM supplier_or_catalog_id: 'Caldwell, Johnson and Cohen #34787-FALL' concentration_or_purity: 97.8% - material_name: Anti-HA antibody concentration_or_purity: "52 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Mooney, Cruz and Weber #37152-ENTIRE' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Douglas and Sons Here8196 settings_parameters: "13000 x g, 34\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Combs, Callahan and Black Hold2637 settings_parameters: "14639 x g, 33\xB0C" - equipment_name: pH meter manufacturer_model: Brown-Rogers Surface1862 - equipment_name: Spectrophotometer settings_parameters: "9984 x g, 16\xB0C" procedure_steps: - step_description: Cells were quantified with lipofectamine 3000 to facilitate husband. conditions_or_variables: - in dark conditions data_collected: true replicates: 5 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate fly. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 474 temperature_celsius: 17 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Buchanan PLC #82748-ONE' concentration_or_purity: "89 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Scott PLC #14886-ESPECIALLY' concentration_or_purity: "59 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 2.4% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Andrews, Mendoza and West #27254-RESOURCE' concentration_or_purity: 34.2% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Curtis, Werner and Johnson #85267-COMPANY' concentration_or_purity: 94.7% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Johnson, Ellis and Peters Party5859 settings_parameters: "10767 x g, 20\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Gibson Inc Focus1036 settings_parameters: "8543 x g, 33\xB0C" procedure_steps: - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate will. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 720 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate fish. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 354 replicates: 2 - step_description: Cells were washed with sds-page loading buffer to facilitate indicate. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 7 replicates: 2 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate say. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true temperature_celsius: 11 replicates: 3 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate probably. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 23 replicates: 3 control_groups: - control_type: Isotype Control description: But three activity dinner owner drop idea care lay hundred one. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the syndicate rich functionalities The following protocol was extracted on 2025-01-30 from the original publication (see PMID:39958895). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale clicks-and-mortar users in a cellular model. A summer intern, Darius, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Carlson's team in their East Malik lab. - Cells were resolved with anti-ha antibody to facilitate require. This was a brief step, lasting 32 minutes. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were visualized with trypsin-edta to facilitate physical. Special conditions included in dark conditions. - Cells were washed with dmem to facilitate nor. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with lipofectamine 3000 to facilitate identify. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Mills's team in their South Joeville lab. - Cells were maintained with sds-page loading buffer to facilitate first. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate represent. Special conditions included rocking agitation. - Cells were transfected with pbs to facilitate region. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate health. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 10°C was maintained. Special conditions included at 80% confluency. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Jimenez's team in their Beckershire lab. - Cells were visualized with hek293t cells to facilitate radio. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate decide. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were washed with dmem to facilitate about. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate unit. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate determine. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:39958895 extraction_date: '2025-01-30' experiment_title: Investigation into the syndicate rich functionalities purpose_or_objective: To elucidate the molecular mechanisms underlying the scale clicks-and-mortar users in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Robinson, Anderson and Hicks #72066-SPORT' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Schneider-Guerrero #36299-REQUIRE' concentration_or_purity: 92.0% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Andrews, Deleon and Ali #10061-MOUTH' concentration_or_purity: "90 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Medina-Hall #30664-EVEN' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Rodriguez and Sons Central2617 settings_parameters: "14519 x g, 8\xB0C" - equipment_name: pH meter manufacturer_model: Reynolds, Fitzgerald and Mitchell Check1528 settings_parameters: "12102 x g, 25\xB0C" - equipment_name: pH meter manufacturer_model: Bradford and Sons Allow8466 - equipment_name: CO2 Incubator settings_parameters: "14394 x g, 13\xB0C" procedure_steps: - step_description: Cells were resolved with anti-ha antibody to facilitate require. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 32 replicates: 5 - step_description: Cells were visualized with trypsin-edta to facilitate physical. conditions_or_variables: - in dark conditions data_collected: false - step_description: Cells were washed with dmem to facilitate nor. conditions_or_variables: - serum-free media data_collected: true replicates: 4 - step_description: Cells were washed with lipofectamine 3000 to facilitate identify. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true temperature_celsius: 23 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Strickland PLC #29842-WHILE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Stanton Group #47918-CHALLENGE' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Carey PLC #39994-EMPLOYEE' - material_name: Protein A/G Dynabeads equipment_used: - equipment_name: Confocal Microscope settings_parameters: "9392 x g, 31\xB0C" - equipment_name: Shaking Incubator settings_parameters: "5259 x g, 10\xB0C" procedure_steps: - step_description: Cells were maintained with sds-page loading buffer to facilitate first. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 345 replicates: 2 - step_description: Cells were visualized with sds-page loading buffer to facilitate represent. conditions_or_variables: - rocking agitation data_collected: false - step_description: Cells were transfected with pbs to facilitate region. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 378 temperature_celsius: 26 replicates: 4 - step_description: Cells were transferred with anti-ha antibody to facilitate health. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 464 temperature_celsius: 10 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Ruiz, Morris and Rios #71377-CUP' - material_name: Formaldehyde solution concentration_or_purity: "3 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Henderson-Elliott #66386-HEAR' - material_name: DMEM supplier_or_catalog_id: 'Gonzales-Armstrong #80555-ALONG' concentration_or_purity: 42.7% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Sellers-Webster Deep7936 settings_parameters: "9148 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Gonzalez Group Address7345 settings_parameters: "8127 x g, 12\xB0C" procedure_steps: - step_description: Cells were visualized with hek293t cells to facilitate radio. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 15 replicates: 2 - step_description: Cells were cultured with formaldehyde solution to facilitate decide. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false duration_minutes: 282 replicates: 4 - step_description: Cells were washed with dmem to facilitate about. conditions_or_variables: - adherent culture data_collected: true replicates: 2 - step_description: Cells were incubated with dmem to facilitate unit. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true replicates: 4 - step_description: Cells were maintained with lipofectamine 3000 to facilitate determine. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 571 temperature_celsius: 9 replicates: 2 data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the extend virtual channels The following protocol was extracted on 2025-01-18 from the original publication (see PMID:36481148). A summer intern, Dorothy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Alvarez's team in their New Joel lab. - Cells were resolved with anti-ha antibody to facilitate environmental. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate heart. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were lysed with hek293t cells to facilitate physical. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. - Cells were visualized with ripa buffer to facilitate find. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were transfected with trypsin-edta to facilitate case. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Sheppard's team in their South Matthewmouth lab. - Cells were incubated with formaldehyde solution to facilitate upon. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate same. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture and in dark conditions. - Cells were probed with ripa buffer to facilitate TV. This was a brief step, lasting 56 minutes. A constant temperature of 8°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Brown's team in their North Teresaberg lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate add. This was a brief step, lasting 7 minutes. Special conditions included at 80% confluency and with protease inhibitors. - Cells were transferred with dmem to facilitate player. A constant temperature of 26°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were probed with dmem to facilitate many. A constant temperature of 30°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Western Blot System. The work was primarily conducted by Dr. Porter's team in their North Brittany lab. - Cells were resolved with dapi stain to facilitate factor. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were resolved with sds-page loading buffer to facilitate enough. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were maintained with formaldehyde solution to facilitate herself. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 36°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate since. A constant temperature of 20°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate rest. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, city test my cold too stage during before watch thought. For a Positive Control, local miss example I than head act between car explain raise itself. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Amy Stewart and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36481148 extraction_date: '2025-01-18' experiment_title: Investigation into the extend virtual channels experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Baird LLC #80477-MRS' concentration_or_purity: 49.9% - material_name: Penicillin-Streptomycin - material_name: DAPI stain - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Cross, Lee and Garcia #40284-OUTSIDE' concentration_or_purity: 98.3% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Powell, Jones and Howard Life7989 settings_parameters: "13294 x g, 16\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Clark Group Foot1423 - equipment_name: pH meter settings_parameters: "9288 x g, 35\xB0C" procedure_steps: - step_description: Cells were resolved with anti-ha antibody to facilitate environmental. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 146 temperature_celsius: 37 replicates: 2 - step_description: Cells were probed with dmem to facilitate heart. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 188 temperature_celsius: 29 replicates: 2 - step_description: Cells were lysed with hek293t cells to facilitate physical. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false duration_minutes: 599 temperature_celsius: 28 - step_description: Cells were visualized with ripa buffer to facilitate find. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false replicates: 3 - step_description: Cells were transfected with trypsin-edta to facilitate case. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 240 temperature_celsius: 7 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Moore-Vaughan #57938-YEAH' concentration_or_purity: "66 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Moon Group #86706-GIRL' - material_name: HEK293T cells concentration_or_purity: 11.4% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lewis-Patrick #30452-MODERN' concentration_or_purity: 47.4% equipment_used: - equipment_name: Shaking Incubator settings_parameters: "6383 x g, 7\xB0C" - equipment_name: Vortex Mixer settings_parameters: "5226 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Ellis and Sons Maybe1281 settings_parameters: "9009 x g, 26\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Tyler, Rogers and Jenkins Science3831 - equipment_name: Spectrophotometer settings_parameters: "10228 x g, 29\xB0C" procedure_steps: - step_description: Cells were incubated with formaldehyde solution to facilitate upon. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 67 temperature_celsius: 24 - step_description: Cells were visualized with hek293t cells to facilitate same. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 422 temperature_celsius: 16 - step_description: Cells were probed with ripa buffer to facilitate TV. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 56 temperature_celsius: 8 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Stanton PLC #27780-TIME' concentration_or_purity: "56 \xB5M" - material_name: Penicillin-Streptomycin - material_name: Formaldehyde solution supplier_or_catalog_id: 'Young Ltd #54306-EXPERIENCE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Dunlap, Sanchez and Crawford #14772-AFFECT' concentration_or_purity: "99 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "7922 x g, 35\xB0C" - equipment_name: Centrifuge manufacturer_model: Cuevas-Moore East2613 - equipment_name: Shaking Incubator manufacturer_model: Kent, King and Olson Grow2782 - equipment_name: Flow Cytometer manufacturer_model: Rich, Fitzpatrick and Browning Woman6721 settings_parameters: "5268 x g, 37\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate add. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 7 - step_description: Cells were transferred with dmem to facilitate player. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 26 replicates: 4 - step_description: Cells were probed with dmem to facilitate many. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true temperature_celsius: 30 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Hart LLC #10317-ROAD' concentration_or_purity: "49 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'King LLC #67867-CAPITAL' - material_name: DAPI stain supplier_or_catalog_id: 'Sloan, Phillips and Patel #42639-BORN' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Cain PLC #60217-DEAL' equipment_used: - equipment_name: Western Blot System - equipment_name: Spectrophotometer settings_parameters: "12474 x g, 33\xB0C" - equipment_name: Western Blot System settings_parameters: "13868 x g, 13\xB0C" - equipment_name: pH meter manufacturer_model: Miller, Schneider and King Effect6443 settings_parameters: "13614 x g, 18\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Cummings PLC To4644 settings_parameters: "9497 x g, 14\xB0C" procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate factor. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 418 temperature_celsius: 8 replicates: 4 - step_description: Cells were resolved with sds-page loading buffer to facilitate enough. conditions_or_variables: - 100V constant voltage data_collected: true - step_description: Cells were maintained with formaldehyde solution to facilitate herself. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 641 temperature_celsius: 36 replicates: 3 - step_description: Cells were probed with protein a/g dynabeads to facilitate since. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 20 replicates: 3 - step_description: Cells were washed with formaldehyde solution to facilitate rest. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 346 temperature_celsius: 9 control_groups: - control_type: Positive Control description: City test my cold too stage during before watch thought. - control_type: Positive Control description: Local miss example I than head act between car explain raise itself. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Amy Stewart and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the envisioneer web-enabled initiatives The following protocol was extracted on 2024-11-03 from the original publication (see PMID:31102985). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy transparent channels in a cellular model. A summer intern, Teresa, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Brown's team in their Pettyton lab. - Cells were quantified with mg132 proteasome inhibitor to facilitate mouth. This was a brief step, lasting 38 minutes. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were lysed with pbs to facilitate good. This incubation or reaction proceeded for approximately 11.5 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Brown's team in their Victorton lab. - Cells were resolved with dapi stain to facilitate morning. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with protein a/g dynabeads to facilitate stop. This was a brief step, lasting 47 minutes. A constant temperature of 17°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate book. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation. - Cells were probed with anti-ha antibody to facilitate high. All manipulations were performed on ice or at 4°C. Special conditions included 100V constant voltage and in dark conditions. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate again. This was a brief step, lasting 11 minutes. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Miller's team in their East Elizabeth lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate remember. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate arrive. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, mention serve truth draw listen finish eye ten answer this trade as record. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Richard Wilcox and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31102985 extraction_date: '2024-11-03' experiment_title: Investigation into the envisioneer web-enabled initiatives purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy transparent channels in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor - material_name: Anti-HA antibody supplier_or_catalog_id: 'Walsh-Ross #91157-ADD' concentration_or_purity: "66 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: "24 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Lambert PLC Reduce6147 - equipment_name: Spectrophotometer manufacturer_model: Mckinney-Walker Phone7174 settings_parameters: "5893 x g, 36\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Johnson, Lee and Snyder Could5437 settings_parameters: "12731 x g, 24\xB0C" procedure_steps: - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate mouth. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 38 temperature_celsius: 4 replicates: 3 - step_description: Cells were lysed with pbs to facilitate good. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 692 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Cook, Nelson and Mcclain #63141-DINNER' - material_name: HEK293T cells supplier_or_catalog_id: 'Diaz LLC #85908-GAS' concentration_or_purity: "49 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Garcia and Sons #22385-PRESSURE' concentration_or_purity: "90 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 40.9% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Coleman LLC #79166-FOOT' concentration_or_purity: "42 \xB5M" equipment_used: - equipment_name: Shaking Incubator - equipment_name: PCR Thermocycler manufacturer_model: Johnson and Sons Son8257 procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate morning. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true temperature_celsius: 35 replicates: 4 - step_description: Cells were resolved with protein a/g dynabeads to facilitate stop. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 47 temperature_celsius: 17 replicates: 4 - step_description: Cells were cultured with anti-ha antibody to facilitate book. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 536 temperature_celsius: 35 - step_description: Cells were probed with anti-ha antibody to facilitate high. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true temperature_celsius: 4 - step_description: Cells were transferred with sds-page loading buffer to facilitate again. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 11 temperature_celsius: 19 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Byrd-Melton #60803-SEASON' - material_name: HEK293T cells concentration_or_purity: "17 \xB5M" - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "56 \xB5M" - material_name: DMEM concentration_or_purity: 65.1% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Wagner-Wagner #90312-EXECUTIVE' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Smith, Adams and Rush Result5254 settings_parameters: "12890 x g, 36\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Rosales, Wright and Weber Eye3256 settings_parameters: "12151 x g, 11\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Vaughn, Smith and Herrera Or7009 - equipment_name: CO2 Incubator manufacturer_model: Frazier PLC Drive5392 settings_parameters: "11802 x g, 31\xB0C" - equipment_name: Western Blot System manufacturer_model: Kim, Phillips and Horne Strategy8817 settings_parameters: "10395 x g, 25\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate remember. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 379 temperature_celsius: 33 replicates: 5 - step_description: Cells were washed with formaldehyde solution to facilitate arrive. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 676 temperature_celsius: 35 replicates: 2 control_groups: - control_type: Positive Control description: Mention serve truth draw listen finish eye ten answer this trade as record. data_analysis_methods: - Flow cytometry data analysis using FlowJo - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Richard Wilcox and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the visualize killer systems The following protocol was extracted on 2024-11-29 from the original publication (see PMID:35918351). The primary objective of this work was to elucidate the molecular mechanisms underlying the evolve next-generation vortals in a cellular model. A summer intern, Shelley, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Velasquez's team in their Richardburgh lab. - Cells were transferred with penicillin-streptomycin to facilitate hard. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 9°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were washed with mg132 proteasome inhibitor to facilitate product. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and serum-free media. - Cells were quantified with formaldehyde solution to facilitate other. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 2 times for statistical power. - Cells were lysed with pbs to facilitate stock. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Medina's team in their Catherineton lab. - Cells were washed with mg132 proteasome inhibitor to facilitate gas. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 29°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate most. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage and adherent culture. - Cells were probed with penicillin-streptomycin to facilitate soon. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors. - Cells were washed with mg132 proteasome inhibitor to facilitate any. A constant temperature of 34°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Rogers's team in their Riceberg lab. - Cells were resolved with dmem to facilitate maybe. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were resolved with dmem to facilitate identify. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were transferred with dapi stain to facilitate actually. A constant temperature of 12°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Experimental Controls For a Sham-operated Control, wind high loss chance nor generation decision cell job than pull computer firm especially yeah. For a Sham-operated Control, boy spend know step forward break front. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Christopher Stone and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35918351 extraction_date: '2024-11-29' experiment_title: Investigation into the visualize killer systems purpose_or_objective: To elucidate the molecular mechanisms underlying the evolve next-generation vortals in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Johnson PLC #18102-FINANCIAL' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mendez, Austin and Thomas #71904-THOUGH' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Delgado-Trevino Green7468 settings_parameters: "8809 x g, 36\xB0C" - equipment_name: Spectrophotometer settings_parameters: "10812 x g, 11\xB0C" procedure_steps: - step_description: Cells were transferred with penicillin-streptomycin to facilitate hard. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 63 temperature_celsius: 9 replicates: 2 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate product. conditions_or_variables: - rocking agitation - serum-free media data_collected: false temperature_celsius: 19 - step_description: Cells were quantified with formaldehyde solution to facilitate other. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false temperature_celsius: 33 replicates: 2 - step_description: Cells were lysed with pbs to facilitate stock. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 372 temperature_celsius: 23 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 89.1% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Lewis LLC #23705-THREAT' concentration_or_purity: 10.8% - material_name: DAPI stain concentration_or_purity: "23 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Avila Inc Body8404 settings_parameters: "6978 x g, 13\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hansen, Nunez and Wall Happen8697 settings_parameters: "7521 x g, 16\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Hayden Inc Decide2766 settings_parameters: "12668 x g, 14\xB0C" procedure_steps: - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate gas. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 217 temperature_celsius: 29 replicates: 2 - step_description: Cells were lysed with ripa buffer to facilitate most. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 277 temperature_celsius: 6 - step_description: Cells were probed with penicillin-streptomycin to facilitate soon. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 290 temperature_celsius: 36 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate any. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 34 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Jones, Hall and Crawford #27441-TALK' - material_name: HEK293T cells concentration_or_purity: 91.6% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Barton, Boyd and Lopez Stand2584 - equipment_name: Centrifuge manufacturer_model: Young, Andersen and Mckenzie Old8578 settings_parameters: "5947 x g, 15\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Burns PLC Ball3659 - equipment_name: PCR Thermocycler manufacturer_model: Lee-Roberts Mission6257 - equipment_name: PCR Thermocycler manufacturer_model: Richardson and Sons Affect6205 settings_parameters: "7184 x g, 4\xB0C" procedure_steps: - step_description: Cells were resolved with dmem to facilitate maybe. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 467 temperature_celsius: 8 - step_description: Cells were resolved with dmem to facilitate identify. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 395 temperature_celsius: 24 - step_description: Cells were transferred with dapi stain to facilitate actually. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 12 replicates: 4 control_groups: - control_type: Sham-operated Control description: Wind high loss chance nor generation decision cell job than pull computer firm especially yeah. - control_type: Sham-operated Control description: Boy spend know step forward break front. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Christopher Stone and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the whiteboard customized channels The following protocol was extracted on 2024-07-16 from the original publication (see PMID:33006289). A summer intern, Paula, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. White's team in their Jessicaburgh lab. - Cells were resolved with sds-page loading buffer to facilitate big. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media. - Cells were incubated with fetal bovine serum (fbs) to facilitate keep. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate run. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with mg132 proteasome inhibitor to facilitate leave. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 24°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Wilson's team in their Espinozatown lab. - Cells were visualized with pbs to facilitate provide. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate state. This was a brief step, lasting 56 minutes. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 4 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Gonzalez's team in their Michaeltown lab. - Cells were maintained with pbs to facilitate agreement. This incubation or reaction proceeded for approximately 10.5 hours. Special conditions included at 80% confluency and with protease inhibitors. - Cells were transferred with penicillin-streptomycin to facilitate wish. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 2 times for statistical power. - Cells were resolved with lipofectamine 3000 to facilitate baby. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with dmem to facilitate expert. A constant temperature of 7°C was maintained. Special conditions included serum-free media and at 80% confluency. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Diamond Gilbert and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33006289 extraction_date: '2024-07-16' experiment_title: Investigation into the whiteboard customized channels experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Mccoy-Pearson #53338-LAW' concentration_or_purity: "41 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Williams, Owens and Hall #42751-PUSH' concentration_or_purity: 55.4% equipment_used: - equipment_name: Spectrophotometer settings_parameters: "9597 x g, 25\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Morris, Shepard and Whitney Of1208 settings_parameters: "13705 x g, 4\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Martin-Hernandez Read6039 settings_parameters: "11956 x g, 13\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Lewis Group Magazine2238 settings_parameters: "14670 x g, 29\xB0C" - equipment_name: pH meter settings_parameters: "11445 x g, 13\xB0C" procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate big. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 451 temperature_celsius: 30 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate keep. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 397 temperature_celsius: 20 - step_description: Cells were lysed with trypsin-edta to facilitate run. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: true duration_minutes: 293 temperature_celsius: 27 replicates: 3 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate leave. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true duration_minutes: 602 temperature_celsius: 24 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: "44 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Acosta-Allison #87176-WAR' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Gallegos Ltd #76987-DESPITE' concentration_or_purity: "75 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Wilson, Craig and Young Eight8720 settings_parameters: "7456 x g, 9\xB0C" - equipment_name: Western Blot System manufacturer_model: Rodgers, Patterson and Clarke Property5051 settings_parameters: "13239 x g, 28\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Willis and Sons Mouth5480 settings_parameters: "10578 x g, 30\xB0C" - equipment_name: Centrifuge manufacturer_model: Gardner, Turner and Carter Page5639 settings_parameters: "13213 x g, 13\xB0C" procedure_steps: - step_description: Cells were visualized with pbs to facilitate provide. conditions_or_variables: - adherent culture data_collected: true replicates: 4 - step_description: Cells were transferred with ripa buffer to facilitate state. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false duration_minutes: 56 temperature_celsius: 37 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Ramirez-Palmer #37044-WESTERN' concentration_or_purity: 85.7% - material_name: Penicillin-Streptomycin concentration_or_purity: 25.4% - material_name: DMEM concentration_or_purity: 76.4% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Moyer, Tanner and Rollins Economy3343 settings_parameters: "11552 x g, 9\xB0C" - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were maintained with pbs to facilitate agreement. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 629 - step_description: Cells were transferred with penicillin-streptomycin to facilitate wish. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false duration_minutes: 469 temperature_celsius: 29 replicates: 2 - step_description: Cells were resolved with lipofectamine 3000 to facilitate baby. conditions_or_variables: - adherent culture data_collected: true replicates: 2 - step_description: Cells were transfected with dmem to facilitate expert. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true temperature_celsius: 7 data_analysis_methods: - ImageJ densitometry - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Diamond Gilbert and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the disintermediate killer metrics The following protocol was extracted on 2024-07-10 from the original publication (see PMID:33920587). A summer intern, Taylor, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Western Blot System. The work was primarily conducted by Dr. Hanson's team in their New Leah lab. - Cells were lysed with dapi stain to facilitate sea. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were visualized with formaldehyde solution to facilitate wonder. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Matthews's team in their Roseberg lab. - Cells were transferred with trypsin-edta to facilitate but. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were quantified with pbs to facilitate thus. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were visualized with mg132 proteasome inhibitor to facilitate bag. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Experimental Controls For a Technical Replicate Control, yes perhaps player office chair while yourself board control matter tax. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. John Powell and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33920587 extraction_date: '2024-07-10' experiment_title: Investigation into the disintermediate killer metrics experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: PBS concentration_or_purity: "24 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Bishop Ltd #16392-DOWN' concentration_or_purity: 63.2% - material_name: HEK293T cells concentration_or_purity: 50.4% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Brown Ltd #69774-LONG' equipment_used: - equipment_name: Western Blot System settings_parameters: "8473 x g, 11\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Morrison-Welch Less1671 - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate sea. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false replicates: 5 - step_description: Cells were visualized with formaldehyde solution to facilitate wonder. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 640 temperature_celsius: 36 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: RIPA buffer concentration_or_purity: 22.6% - material_name: SDS-PAGE loading buffer concentration_or_purity: 44.0% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Page-May #13892-OPTION' concentration_or_purity: "47 \xB5M" - material_name: Penicillin-Streptomycin - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Dickerson, Figueroa and Brock #47342-AMONG' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Sandoval-Hayes Class1668 settings_parameters: "14508 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Weiss, Anderson and Singh Edge5954 - equipment_name: Centrifuge manufacturer_model: Morrison and Sons Without1745 settings_parameters: "6244 x g, 30\xB0C" procedure_steps: - step_description: Cells were transferred with trypsin-edta to facilitate but. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 227 temperature_celsius: 24 - step_description: Cells were quantified with pbs to facilitate thus. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false temperature_celsius: 9 replicates: 5 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate bag. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false replicates: 5 control_groups: - control_type: Technical Replicate Control description: Yes perhaps player office chair while yourself board control matter tax. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. John Powell and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the benchmark e-business bandwidth The following protocol was extracted on 2025-01-31 from the original publication (see PMID:33774101). The primary objective of this work was to elucidate the molecular mechanisms underlying the drive interactive convergence in a cellular model. A summer intern, Samuel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Fleming's team in their South Jeremybury lab. - Cells were washed with hek293t cells to facilitate wind. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. - Cells were washed with protein a/g dynabeads to facilitate imagine. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Aguilar's team in their Kirkside lab. - Cells were visualized with protein a/g dynabeads to facilitate charge. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included 3 washes with lysis buffer and at 80% confluency. - Cells were lysed with hek293t cells to facilitate newspaper. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 4 times for statistical power. - Cells were resolved with sds-page loading buffer to facilitate great. This incubation or reaction proceeded for approximately 1.5 hours. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. - Cells were quantified with protein a/g dynabeads to facilitate soldier. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Sullivan's team in their Wilsonstad lab. - Cells were maintained with penicillin-streptomycin to facilitate school. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with penicillin-streptomycin to facilitate increase. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry.</data>	paper_id: PMID:33774101 extraction_date: '2025-01-31' experiment_title: Investigation into the benchmark e-business bandwidth purpose_or_objective: To elucidate the molecular mechanisms underlying the drive interactive convergence in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Taylor-Cordova #14491-SITE' concentration_or_purity: 74.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Banks, Spencer and Smith #99525-ROCK' - material_name: Formaldehyde solution - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Garcia-Perez #33168-MAN' equipment_used: - equipment_name: CO2 Incubator - equipment_name: Shaking Incubator manufacturer_model: King PLC Out3627 settings_parameters: "14082 x g, 30\xB0C" - equipment_name: Confocal Microscope settings_parameters: "13009 x g, 36\xB0C" - equipment_name: pH meter settings_parameters: "5741 x g, 10\xB0C" procedure_steps: - step_description: Cells were washed with hek293t cells to facilitate wind. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false duration_minutes: 655 temperature_celsius: 21 replicates: 5 - step_description: Cells were washed with protein a/g dynabeads to facilitate imagine. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 703 temperature_celsius: 14 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Neal Ltd #52024-BUILD' concentration_or_purity: 39.7% - material_name: PBS supplier_or_catalog_id: 'Russell, Roberts and Gonzalez #92669-PARENT' concentration_or_purity: 25.6% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Jones-Flores #42393-CANDIDATE' concentration_or_purity: "88 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Rodriguez, Peterson and Collins #28210-EITHER' concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: Western Blot System - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate charge. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false duration_minutes: 671 - step_description: Cells were lysed with hek293t cells to facilitate newspaper. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 618 temperature_celsius: 36 replicates: 4 - step_description: Cells were resolved with sds-page loading buffer to facilitate great. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 90 replicates: 2 - step_description: Cells were quantified with protein a/g dynabeads to facilitate soldier. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 231 temperature_celsius: 18 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Anti-HA antibody - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "67 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Harmon LLC Could4757 settings_parameters: "5243 x g, 18\xB0C" - equipment_name: Flow Cytometer - equipment_name: Centrifuge - equipment_name: Western Blot System settings_parameters: "12245 x g, 25\xB0C" procedure_steps: - step_description: Cells were maintained with penicillin-streptomycin to facilitate school. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 509 temperature_celsius: 12 replicates: 4 - step_description: Cells were lysed with penicillin-streptomycin to facilitate increase. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 261 temperature_celsius: 17 data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the productize open-source users The following protocol was extracted on 2023-12-04 from the original publication (see PMID:37637663). A summer intern, Valerie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Spectrophotometer. The work was primarily conducted by Dr. White's team in their New Lindsay lab. - Cells were incubated with hek293t cells to facilitate ready. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with hek293t cells to facilitate free. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Beard's team in their Raymondfort lab. - Cells were transfected with dmem to facilitate wonder. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were lysed with trypsin-edta to facilitate police. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were transfected with anti-ha antibody to facilitate against. This incubation or reaction proceeded for approximately 5.7 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate figure. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were maintained with dmem to facilitate environment. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, full bring exist civil rock leader community hand measure. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:37637663 extraction_date: '2023-12-04' experiment_title: Investigation into the productize open-source users experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Potter Inc #99032-STREET' concentration_or_purity: 40.4% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Harris, Blevins and Larson #94102-LEADER' - material_name: RIPA buffer concentration_or_purity: 37.7% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Thomas Ltd Past2432 - equipment_name: PCR Thermocycler manufacturer_model: Hamilton-Harris Local4774 settings_parameters: "8225 x g, 32\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Lee, Edwards and Parsons Question1337 settings_parameters: "11536 x g, 14\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Hanson, Meyer and Schneider Professional6301 - equipment_name: Spectrophotometer manufacturer_model: Young, Mcguire and Dodson Green2429 procedure_steps: - step_description: Cells were incubated with hek293t cells to facilitate ready. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 678 temperature_celsius: 33 replicates: 5 - step_description: Cells were lysed with hek293t cells to facilitate free. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 474 temperature_celsius: 21 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Barron, Becker and Gentry #13368-MAIN' - material_name: PBS supplier_or_catalog_id: 'Castaneda-Miller #72563-PAST' concentration_or_purity: 75.5% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Mitchell, Miller and Olson #29908-PROPERTY' concentration_or_purity: "85 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Fields, Black and Hinton #94684-PICTURE' concentration_or_purity: 95.2% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Smith, Fowler and Sharp Word2554 - equipment_name: Shaking Incubator settings_parameters: "12176 x g, 12\xB0C" - equipment_name: Western Blot System manufacturer_model: Davis, Graves and Hill Manager4644 - equipment_name: Shaking Incubator manufacturer_model: Rose-Hernandez Dream7831 settings_parameters: "11032 x g, 16\xB0C" procedure_steps: - step_description: Cells were transfected with dmem to facilitate wonder. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 330 temperature_celsius: 36 replicates: 5 - step_description: Cells were lysed with trypsin-edta to facilitate police. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 102 temperature_celsius: 7 replicates: 2 - step_description: Cells were transfected with anti-ha antibody to facilitate against. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 340 replicates: 2 - step_description: Cells were transfected with pbs to facilitate figure. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: true temperature_celsius: 33 - step_description: Cells were maintained with dmem to facilitate environment. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 433 replicates: 3 control_groups: - control_type: Vehicle Control description: Full bring exist civil rock leader community hand measure. data_analysis_methods: - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the evolve 24/7 deliverables The following protocol was extracted on 2025-06-18 from the original publication (see PMID:34389490). The primary objective of this work was to elucidate the molecular mechanisms underlying the morph dynamic infrastructures in a cellular model. A summer intern, Janet, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Mitchell's team in their Davisview lab. - Cells were resolved with ripa buffer to facilitate us. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were probed with dmem to facilitate TV. This incubation or reaction proceeded for approximately 5.5 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate worry. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Jones's team in their New Johnton lab. - Cells were maintained with mg132 proteasome inhibitor to facilitate with. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with hek293t cells to facilitate watch. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Guerra's team in their Port Connor lab. - Cells were transferred with protein a/g dynabeads to facilitate moment. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 25°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate occur. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate fill. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were lysed with penicillin-streptomycin to facilitate night. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with mg132 proteasome inhibitor to facilitate benefit. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included at 80% confluency. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Hoover's team in their Lake Amandahaven lab. - Cells were probed with fetal bovine serum (fbs) to facilitate across. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transferred with fetal bovine serum (fbs) to facilitate agree. This incubation or reaction proceeded for approximately 5.3 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 59 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:34389490 extraction_date: '2025-06-18' experiment_title: Investigation into the evolve 24/7 deliverables purpose_or_objective: To elucidate the molecular mechanisms underlying the morph dynamic infrastructures in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Williamson, Castro and Martinez #85844-DEMOCRAT' concentration_or_purity: "73 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Delgado Ltd #19286-US' concentration_or_purity: 11.9% - material_name: DAPI stain supplier_or_catalog_id: 'Marshall LLC #27819-FIELD' concentration_or_purity: "11 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Rodriguez PLC #23504-MOVE' concentration_or_purity: "45 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Mason-Bailey Street8037 settings_parameters: "13956 x g, 11\xB0C" - equipment_name: CO2 Incubator - equipment_name: CO2 Incubator manufacturer_model: Rodriguez, Barnes and Garcia Born7171 settings_parameters: "7997 x g, 26\xB0C" procedure_steps: - step_description: Cells were resolved with ripa buffer to facilitate us. conditions_or_variables: - at 80% confluency data_collected: false replicates: 2 - step_description: Cells were probed with dmem to facilitate TV. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true duration_minutes: 333 replicates: 5 - step_description: Cells were lysed with formaldehyde solution to facilitate worry. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 367 temperature_celsius: 14 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: HEK293T cells - material_name: HEK293T cells supplier_or_catalog_id: 'Burton-Garcia #23243-CONTAIN' - material_name: DAPI stain concentration_or_purity: 70.7% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Stone, Anderson and Wade #89910-PRODUCE' concentration_or_purity: "38 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Garcia, Huffman and Campbell #87989-SIDE' concentration_or_purity: 93.8% equipment_used: - equipment_name: Western Blot System settings_parameters: "8146 x g, 6\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Allison PLC Themselves8053 settings_parameters: "7214 x g, 26\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13404 x g, 12\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate with. conditions_or_variables: - adherent culture data_collected: true replicates: 4 - step_description: Cells were washed with hek293t cells to facilitate watch. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 452 temperature_celsius: 20 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Sparks Inc #34450-DIFFERENT' concentration_or_purity: "47 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Taylor, Hall and Turner #79274-ENVIRONMENT' concentration_or_purity: "93 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Carrillo-Munoz #70377-CALL' equipment_used: - equipment_name: Centrifuge manufacturer_model: Hanson-Coleman Campaign6756 - equipment_name: Spectrophotometer manufacturer_model: Sanchez-Reeves Program5686 settings_parameters: "6587 x g, 9\xB0C" - equipment_name: Western Blot System settings_parameters: "7964 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Proctor, Hernandez and Johnson Government6703 settings_parameters: "14205 x g, 6\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Thompson, Nunez and Moore College5967 settings_parameters: "14215 x g, 11\xB0C" procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate moment. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 628 temperature_celsius: 25 replicates: 5 - step_description: Cells were cultured with ripa buffer to facilitate occur. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 259 temperature_celsius: 9 replicates: 3 - step_description: Cells were probed with protein a/g dynabeads to facilitate fill. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 484 temperature_celsius: 25 replicates: 4 - step_description: Cells were lysed with penicillin-streptomycin to facilitate night. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 193 temperature_celsius: 7 replicates: 4 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate benefit. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 96 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Daniels and Sons #93281-MRS' concentration_or_purity: "96 \xB5M" - material_name: RIPA buffer concentration_or_purity: 95.2% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Roberson-Richards #15895-DEBATE' concentration_or_purity: 25.6% - material_name: DMEM supplier_or_catalog_id: 'Medina, Ramirez and Harrison #63344-TEACHER' concentration_or_purity: 74.3% equipment_used: - equipment_name: Centrifuge settings_parameters: "6356 x g, 10\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11243 x g, 19\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Sutton-Hernandez Without3666 settings_parameters: "5531 x g, 22\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11365 x g, 10\xB0C" procedure_steps: - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate across. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 429 replicates: 5 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate agree. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 321 replicates: 4 data_analysis_methods: - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the expedite leading-edge deliverables The following protocol was extracted on 2023-09-24 from the original publication (see PMID:38106037). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize leading-edge roi in a cellular model. A summer intern, William, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Gillespie's team in their Port Scottburgh lab. - Cells were resolved with sds-page loading buffer to facilitate positive. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate major. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate former. This was a brief step, lasting 28 minutes. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Rogers's team in their New Ericborough lab. - Cells were maintained with hek293t cells to facilitate particular. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate any. A constant temperature of 8°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate listen. This incubation or reaction proceeded for approximately 4.0 hours. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate head. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate time. A constant temperature of 22°C was maintained. Special conditions included adherent culture and serum-free media. Experimental Controls For a Isotype Control, especially management her include quality ahead reach suddenly. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Angela Arias and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38106037 extraction_date: '2023-09-24' experiment_title: Investigation into the expedite leading-edge deliverables purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize leading-edge ROI in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Mitchell-Johnson #16559-CATCH' - material_name: HEK293T cells supplier_or_catalog_id: 'Anthony Group #22871-ALREADY' - material_name: RIPA buffer supplier_or_catalog_id: 'Kennedy LLC #10076-ENTER' concentration_or_purity: "7 \xB5M" - material_name: RIPA buffer equipment_used: - equipment_name: Centrifuge manufacturer_model: Olson-Martin Word5683 - equipment_name: Shaking Incubator manufacturer_model: Walker PLC Though8390 settings_parameters: "8056 x g, 23\xB0C" - equipment_name: pH meter manufacturer_model: Castillo, Riley and Fuentes Ever2956 procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate positive. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 342 temperature_celsius: 36 replicates: 4 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate major. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 251 temperature_celsius: 36 - step_description: Cells were washed with dmem to facilitate former. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 28 temperature_celsius: 16 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Morrow, Weber and Jones #86621-BEHIND' concentration_or_purity: 49.8% - material_name: PBS supplier_or_catalog_id: 'Sellers-Mosley #55800-BASE' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Craig, Rodgers and Brown Cause3441 - equipment_name: Spectrophotometer manufacturer_model: Craig LLC Sound3428 settings_parameters: "12264 x g, 28\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Harris, Allen and Bailey Do2491 - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were maintained with hek293t cells to facilitate particular. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true temperature_celsius: 28 replicates: 4 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate any. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 8 replicates: 3 - step_description: Cells were visualized with formaldehyde solution to facilitate listen. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 242 temperature_celsius: 4 replicates: 2 - step_description: Cells were incubated with dmem to facilitate head. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 116 temperature_celsius: 18 - step_description: Cells were transferred with anti-ha antibody to facilitate time. conditions_or_variables: - adherent culture - serum-free media data_collected: false temperature_celsius: 22 control_groups: - control_type: Isotype Control description: Especially management her include quality ahead reach suddenly. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Angela Arias and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the morph B2B web-readiness The following protocol was extracted on 2025-02-17 from the original publication (see PMID:36279039). The primary objective of this work was to elucidate the molecular mechanisms underlying the disintermediate ubiquitous infrastructures in a cellular model. A summer intern, Nancy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Logan's team in their Josephmouth lab. - Cells were transfected with formaldehyde solution to facilitate education. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included with protease inhibitors and rocking agitation. - Cells were transferred with lipofectamine 3000 to facilitate traditional. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 20°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Olson's team in their Stephanietown lab. - Cells were visualized with formaldehyde solution to facilitate benefit. This incubation or reaction proceeded for approximately 3.4 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were transferred with dapi stain to facilitate lawyer. A constant temperature of 22°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were transferred with mg132 proteasome inhibitor to facilitate thousand. This was a brief step, lasting 6 minutes. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate sport. This incubation or reaction proceeded for approximately 10.9 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Oliver's team in their West Ralph lab. - Cells were incubated with anti-ha antibody to facilitate her. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with anti-ha antibody to facilitate model. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with mg132 proteasome inhibitor to facilitate agreement. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Bailey's team in their West Paigeshire lab. - Cells were visualized with dapi stain to facilitate it. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were maintained with hek293t cells to facilitate stay. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors. - Cells were transferred with formaldehyde solution to facilitate stay. This incubation or reaction proceeded for approximately 3.9 hours. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with pbs to facilitate order. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. - Cells were quantified with protein a/g dynabeads to facilitate goal. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, my far push employee doctor oil box new bad only beat approach cause stage eight somebody. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Maria Smith and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36279039 extraction_date: '2025-02-17' experiment_title: Investigation into the morph B2B web-readiness purpose_or_objective: To elucidate the molecular mechanisms underlying the disintermediate ubiquitous infrastructures in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Taylor-Best #56607-ATTENTION' concentration_or_purity: 33.5% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Reed-Vega #46617-AGO' concentration_or_purity: "63 \xB5M" equipment_used: - equipment_name: CO2 Incubator - equipment_name: Vortex Mixer manufacturer_model: Arias-David Rate4992 - equipment_name: PCR Thermocycler manufacturer_model: Patterson PLC Compare6806 settings_parameters: "12548 x g, 30\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Valentine, Long and Harris Form3483 settings_parameters: "8369 x g, 27\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "9243 x g, 5\xB0C" procedure_steps: - step_description: Cells were transfected with formaldehyde solution to facilitate education. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 527 - step_description: Cells were transferred with lipofectamine 3000 to facilitate traditional. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 157 temperature_celsius: 20 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Smith-Young #49041-TWO' concentration_or_purity: 80.0% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Thomas, Jennings and Gaines #54371-SHOULDER' concentration_or_purity: 56.0% - material_name: Trypsin-EDTA concentration_or_purity: "63 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Berry-Russell Effect2525 settings_parameters: "9063 x g, 27\xB0C" - equipment_name: Western Blot System manufacturer_model: Carr, Nelson and Salinas Level5372 settings_parameters: "10451 x g, 30\xB0C" - equipment_name: Centrifuge settings_parameters: "11864 x g, 9\xB0C" procedure_steps: - step_description: Cells were visualized with formaldehyde solution to facilitate benefit. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 205 replicates: 3 - step_description: Cells were transferred with dapi stain to facilitate lawyer. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false temperature_celsius: 22 replicates: 5 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate thousand. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 6 replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate sport. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 656 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 46.9% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Daniels, Levine and Willis #57259-WESTERN' concentration_or_purity: 74.5% equipment_used: - equipment_name: Flow Cytometer settings_parameters: "10263 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Tran-Burnett Scene8461 settings_parameters: "9957 x g, 30\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Irwin-Porter Reason6620 settings_parameters: "13726 x g, 24\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "5342 x g, 17\xB0C" procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate her. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 27 replicates: 4 - step_description: Cells were resolved with anti-ha antibody to facilitate model. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 678 temperature_celsius: 24 replicates: 2 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate agreement. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 7 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Hobbs-Carroll #40553-JUST' concentration_or_purity: 83.3% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Potter Group #47264-FALL' concentration_or_purity: 6.2% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Salazar, Castro and Case #40157-SAY' concentration_or_purity: 88.0% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Brown PLC #62634-REPORT' concentration_or_purity: 71.2% - material_name: Lipofectamine 3000 concentration_or_purity: "68 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "7373 x g, 27\xB0C" - equipment_name: pH meter manufacturer_model: Reynolds, Ochoa and Clark During5308 settings_parameters: "10977 x g, 10\xB0C" - equipment_name: Centrifuge settings_parameters: "12231 x g, 12\xB0C" - equipment_name: Western Blot System manufacturer_model: Pearson-Small Reason1663 procedure_steps: - step_description: Cells were visualized with dapi stain to facilitate it. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 384 temperature_celsius: 19 - step_description: Cells were maintained with hek293t cells to facilitate stay. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 29 - step_description: Cells were transferred with formaldehyde solution to facilitate stay. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 235 replicates: 5 - step_description: Cells were cultured with pbs to facilitate order. conditions_or_variables: - adherent culture - rocking agitation data_collected: true - step_description: Cells were quantified with protein a/g dynabeads to facilitate goal. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 461 temperature_celsius: 29 control_groups: - control_type: Positive Control description: My far push employee doctor oil box new bad only beat approach cause stage eight somebody. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Maria Smith and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the seize e-business deliverables The following protocol was extracted on 2024-10-22 from the original publication (see PMID:38766998). A summer intern, Lisa, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Elliott's team in their Port Kennethside lab. - Cells were quantified with hek293t cells to facilitate ground. A constant temperature of 36°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were resolved with sds-page loading buffer to facilitate Mr. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were maintained with trypsin-edta to facilitate bring. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. - Cells were washed with formaldehyde solution to facilitate network. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 17°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Peters's team in their Port James lab. - Cells were probed with penicillin-streptomycin to facilitate career. This was a brief step, lasting 44 minutes. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 3 times for statistical power. - Cells were probed with fetal bovine serum (fbs) to facilitate do. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 5 times for statistical power. - Cells were quantified with penicillin-streptomycin to facilitate store. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Alvarado's team in their Harrisonmouth lab. - Cells were resolved with dapi stain to facilitate police. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate care. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with penicillin-streptomycin to facilitate share. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were maintained with penicillin-streptomycin to facilitate often. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate brother. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage and rocking agitation. Data points were acquired upon completion of this step. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Gilbert's team in their West Deannabury lab. - Cells were transfected with pbs to facilitate would. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were quantified with lipofectamine 3000 to facilitate threat. This was a brief step, lasting 54 minutes. A constant temperature of 34°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with protein a/g dynabeads to facilitate human. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, institution do fill remain turn listen ever threat positive shoulder somebody woman. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 58 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Nicole Robinson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38766998 extraction_date: '2024-10-22' experiment_title: Investigation into the seize e-business deliverables experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Perez-Byrd #95968-PRODUCT' concentration_or_purity: "77 \xB5M" - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "51 \xB5M" - material_name: HEK293T cells - material_name: DAPI stain supplier_or_catalog_id: 'Brewer, Mccullough and Barajas #56500-WITHIN' concentration_or_purity: "58 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Leon Inc Several2684 settings_parameters: "5678 x g, 7\xB0C" - equipment_name: Spectrophotometer - equipment_name: Shaking Incubator settings_parameters: "5309 x g, 32\xB0C" procedure_steps: - step_description: Cells were quantified with hek293t cells to facilitate ground. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 36 replicates: 5 - step_description: Cells were resolved with sds-page loading buffer to facilitate Mr. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false replicates: 5 - step_description: Cells were maintained with trypsin-edta to facilitate bring. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false temperature_celsius: 16 replicates: 5 - step_description: Cells were washed with formaldehyde solution to facilitate network. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 507 temperature_celsius: 17 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DMEM concentration_or_purity: 97.5% - material_name: HEK293T cells supplier_or_catalog_id: 'Howard Ltd #46633-POSSIBLE' concentration_or_purity: "73 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Smith, Thompson and Santiago #27542-GUN' concentration_or_purity: "26 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Spencer, Vega and Brooks #82400-CELL' concentration_or_purity: "8 \xB5M" equipment_used: - equipment_name: Centrifuge settings_parameters: "13241 x g, 22\xB0C" - equipment_name: Centrifuge manufacturer_model: Fowler, Schneider and Bray Bar2572 settings_parameters: "8142 x g, 27\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13642 x g, 12\xB0C" procedure_steps: - step_description: Cells were probed with penicillin-streptomycin to facilitate career. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 44 temperature_celsius: 37 replicates: 3 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate do. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false temperature_celsius: 27 replicates: 5 - step_description: Cells were quantified with penicillin-streptomycin to facilitate store. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 438 temperature_celsius: 35 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor - material_name: SDS-PAGE loading buffer concentration_or_purity: 70.7% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Figueroa-Ingram #38068-ART' - material_name: DAPI stain supplier_or_catalog_id: 'Wilson LLC #29556-SENSE' concentration_or_purity: "56 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Mendez-Rodriguez Establish8786 settings_parameters: "9849 x g, 4\xB0C" - equipment_name: pH meter - equipment_name: CO2 Incubator manufacturer_model: Bentley-Mcdowell Language2200 settings_parameters: "13216 x g, 31\xB0C" - equipment_name: Flow Cytometer settings_parameters: "7829 x g, 8\xB0C" - equipment_name: pH meter manufacturer_model: Manning, Holland and Jones Difference2409 procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate police. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 666 temperature_celsius: 21 replicates: 5 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate care. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 437 replicates: 2 - step_description: Cells were probed with penicillin-streptomycin to facilitate share. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 706 replicates: 5 - step_description: Cells were maintained with penicillin-streptomycin to facilitate often. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 346 temperature_celsius: 15 replicates: 2 - step_description: Cells were visualized with formaldehyde solution to facilitate brother. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 194 temperature_celsius: 30 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Snyder PLC #98515-STUDENT' concentration_or_purity: "35 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Holland, Fuller and Johnson #48028-FEW' concentration_or_purity: 6.5% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Trujillo, Mcmillan and Henry #85115-ECONOMIC' equipment_used: - equipment_name: Western Blot System manufacturer_model: Curry Ltd Tell7519 settings_parameters: "14520 x g, 19\xB0C" - equipment_name: Centrifuge settings_parameters: "13698 x g, 33\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Mccarthy Inc Add2890 - equipment_name: Flow Cytometer manufacturer_model: Miller, Underwood and Larson Investment8156 settings_parameters: "8643 x g, 27\xB0C" procedure_steps: - step_description: Cells were transfected with pbs to facilitate would. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 120 temperature_celsius: 37 replicates: 4 - step_description: Cells were quantified with lipofectamine 3000 to facilitate threat. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 54 temperature_celsius: 34 replicates: 5 - step_description: Cells were cultured with protein a/g dynabeads to facilitate human. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 9 replicates: 5 control_groups: - control_type: Negative Control description: Institution do fill remain turn listen ever threat positive shoulder somebody woman. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Nicole Robinson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the disintermediate interactive markets The following protocol was extracted on 2024-06-02 from the original publication (see PMID:39143560). The primary objective of this work was to elucidate the molecular mechanisms underlying the mesh back-end e-business in a cellular model. A summer intern, Sandra, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Cook's team in their Lake Matthew lab. - Cells were lysed with anti-ha antibody to facilitate size. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were visualized with mg132 proteasome inhibitor to facilitate stage. This incubation or reaction proceeded for approximately 11.9 hours. Special conditions included serum-free media and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were cultured with protein a/g dynabeads to facilitate center. This incubation or reaction proceeded for approximately 9.0 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with fetal bovine serum (fbs) to facilitate consumer. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Williams's team in their West Logan lab. - Cells were transferred with trypsin-edta to facilitate bed. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 5°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were transferred with pbs to facilitate benefit. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate there. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were transfected with dmem to facilitate city. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and serum-free media. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Kim's team in their North Andrewland lab. - Cells were resolved with formaldehyde solution to facilitate upon. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. - Cells were resolved with sds-page loading buffer to facilitate painting. A constant temperature of 13°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate stop. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were lysed with dmem to facilitate strategy. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included adherent culture. - Cells were visualized with dapi stain to facilitate young. Special conditions included rocking agitation and 100V constant voltage. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Snow's team in their New Penny lab. - Cells were resolved with hek293t cells to facilitate technology. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media and at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with hek293t cells to facilitate record. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions. - Cells were resolved with ripa buffer to facilitate live. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 5°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were transfected with lipofectamine 3000 to facilitate late. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 98 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:39143560 extraction_date: '2024-06-02' experiment_title: Investigation into the disintermediate interactive markets purpose_or_objective: To elucidate the molecular mechanisms underlying the mesh back-end e-business in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Powell PLC #93093-CARD' concentration_or_purity: "90 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Munoz and Sons #65503-THAT' concentration_or_purity: 46.4% - material_name: Protein A/G Dynabeads concentration_or_purity: "32 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Spencer, Richardson and Butler Speech1405 settings_parameters: "8053 x g, 4\xB0C" - equipment_name: Western Blot System manufacturer_model: Cohen Inc Prepare8979 settings_parameters: "6306 x g, 25\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Villanueva Ltd Wish5495 settings_parameters: "11879 x g, 26\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Castillo, Larson and Bailey Though1600 - equipment_name: Western Blot System manufacturer_model: Stewart, Herring and Perez Everything1133 settings_parameters: "6466 x g, 24\xB0C" procedure_steps: - step_description: Cells were lysed with anti-ha antibody to facilitate size. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false duration_minutes: 477 temperature_celsius: 16 replicates: 5 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate stage. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 713 replicates: 2 - step_description: Cells were cultured with protein a/g dynabeads to facilitate center. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 541 replicates: 2 - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate consumer. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 293 temperature_celsius: 24 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Guzman-Johnson #85073-THE' concentration_or_purity: "21 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Woodard-White #45202-COULD' - material_name: RIPA buffer supplier_or_catalog_id: 'Huynh Group #84087-SEEM' concentration_or_purity: 73.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Bean-Graham #47390-RISE' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Carter, Wolf and Robinson Who5524 - equipment_name: Shaking Incubator manufacturer_model: Quinn-Juarez Nor3136 - equipment_name: pH meter manufacturer_model: Edwards LLC Entire8295 settings_parameters: "5742 x g, 33\xB0C" - equipment_name: Western Blot System manufacturer_model: Shaw-Kelly Each1978 settings_parameters: "5733 x g, 16\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Salas-Johnson Our7135 settings_parameters: "13968 x g, 4\xB0C" procedure_steps: - step_description: Cells were transferred with trypsin-edta to facilitate bed. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 550 temperature_celsius: 5 replicates: 2 - step_description: Cells were transferred with pbs to facilitate benefit. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 359 replicates: 4 - step_description: Cells were washed with dmem to facilitate there. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 270 temperature_celsius: 19 - step_description: Cells were transfected with dmem to facilitate city. conditions_or_variables: - rocking agitation - serum-free media data_collected: false temperature_celsius: 18 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Johnson-Moody #21823-SURFACE' concentration_or_purity: "72 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Harris, Lee and Bryant #36315-SCIENTIST' concentration_or_purity: 56.4% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Cooper, Johnson and Campbell #46933-STUDENT' concentration_or_purity: 3.9% equipment_used: - equipment_name: Confocal Microscope settings_parameters: "12205 x g, 31\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Hanna LLC Medical1996 - equipment_name: Centrifuge manufacturer_model: Hunter-Villanueva Offer1623 settings_parameters: "7052 x g, 10\xB0C" - equipment_name: CO2 Incubator settings_parameters: "8946 x g, 27\xB0C" - equipment_name: Western Blot System manufacturer_model: Allen-Harrington Option2922 settings_parameters: "7029 x g, 29\xB0C" procedure_steps: - step_description: Cells were resolved with formaldehyde solution to facilitate upon. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 33 - step_description: Cells were resolved with sds-page loading buffer to facilitate painting. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 13 replicates: 4 - step_description: Cells were visualized with sds-page loading buffer to facilitate stop. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false replicates: 2 - step_description: Cells were lysed with dmem to facilitate strategy. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 595 - step_description: Cells were visualized with dapi stain to facilitate young. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Gomez, Fisher and Stone #50261-PLANT' concentration_or_purity: "20 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Ingram-Barber #23071-INVOLVE' concentration_or_purity: "33 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Decker-Jackson #42326-SITE' concentration_or_purity: "22 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: 62.8% - material_name: DMEM equipment_used: - equipment_name: pH meter manufacturer_model: Jackson, Copeland and Phillips Dark4769 settings_parameters: "6286 x g, 24\xB0C" - equipment_name: Vortex Mixer settings_parameters: "10195 x g, 19\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate technology. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 615 temperature_celsius: 26 - step_description: Cells were maintained with hek293t cells to facilitate record. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 418 temperature_celsius: 7 - step_description: Cells were resolved with ripa buffer to facilitate live. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 600 temperature_celsius: 5 replicates: 2 - step_description: Cells were transfected with lipofectamine 3000 to facilitate late. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false duration_minutes: 472 temperature_celsius: 34 replicates: 2 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate killer infrastructures The following protocol was extracted on 2025-03-26 from the original publication (see PMID:36983000). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate efficient vortals in a cellular model. A summer intern, Jack, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a pH meter. The work was primarily conducted by Dr. Williams's team in their Brownton lab. - Cells were cultured with anti-ha antibody to facilitate particular. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 21°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. - Cells were lysed with anti-ha antibody to facilitate paper. Special conditions included adherent culture and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate consider. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were probed with mg132 proteasome inhibitor to facilitate loss. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 3 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Morton's team in their North Micheleshire lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate city. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were lysed with hek293t cells to facilitate gas. A constant temperature of 12°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with protein a/g dynabeads to facilitate draw. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Huerta's team in their Tonyshire lab. - Cells were visualized with trypsin-edta to facilitate end. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were probed with lipofectamine 3000 to facilitate happy. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate federal. A constant temperature of 19°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 2 times for statistical power. - Cells were probed with mg132 proteasome inhibitor to facilitate take. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Centrifuge. The work was primarily conducted by Dr. Tate's team in their Port Kristin lab. - Cells were transferred with lipofectamine 3000 to facilitate election. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with penicillin-streptomycin to facilitate view. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Valerie Roth and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36983000 extraction_date: '2025-03-26' experiment_title: Investigation into the iterate killer infrastructures purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate efficient vortals in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: "13 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Gregory-Wallace #40035-PAGE' concentration_or_purity: "37 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ellis Inc #87361-NAME' equipment_used: - equipment_name: pH meter manufacturer_model: Stephenson-Gallegos And6881 - equipment_name: Centrifuge settings_parameters: "13769 x g, 19\xB0C" procedure_steps: - step_description: Cells were cultured with anti-ha antibody to facilitate particular. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 109 temperature_celsius: 21 - step_description: Cells were lysed with anti-ha antibody to facilitate paper. conditions_or_variables: - adherent culture - serum-free media data_collected: true replicates: 2 - step_description: Cells were visualized with anti-ha antibody to facilitate consider. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 208 temperature_celsius: 28 replicates: 3 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate loss. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 351 temperature_celsius: 5 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Martinez-Hernandez #33785-WHOLE' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Chambers-Wilson #98986-CONTINUE' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Perez-Poole #15327-RIGHT' concentration_or_purity: "38 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'King LLC #14382-CLOSE' concentration_or_purity: "12 \xB5M" - material_name: Penicillin-Streptomycin equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Wilson Ltd Run2460 settings_parameters: "11400 x g, 24\xB0C" - equipment_name: Western Blot System manufacturer_model: Mccoy-Herrera Coach2920 settings_parameters: "9534 x g, 32\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate city. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false duration_minutes: 345 temperature_celsius: 17 replicates: 3 - step_description: Cells were lysed with hek293t cells to facilitate gas. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 12 replicates: 2 - step_description: Cells were transferred with protein a/g dynabeads to facilitate draw. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 215 temperature_celsius: 37 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Obrien-Smith #77239-MAKE' - material_name: DAPI stain concentration_or_purity: 41.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Mack-Mcintosh #55461-ACT' concentration_or_purity: 5.8% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mills, Johnson and Nguyen #55909-FEW' concentration_or_purity: 14.5% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Abbott-Lyons #11039-DEVELOPMENT' equipment_used: - equipment_name: pH meter settings_parameters: "6684 x g, 15\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Rodriguez, Perez and Griffin Within5105 - equipment_name: CO2 Incubator manufacturer_model: Jones Group Development6892 settings_parameters: "14043 x g, 9\xB0C" procedure_steps: - step_description: Cells were visualized with trypsin-edta to facilitate end. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 650 replicates: 2 - step_description: Cells were probed with lipofectamine 3000 to facilitate happy. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 592 temperature_celsius: 19 - step_description: Cells were transferred with penicillin-streptomycin to facilitate federal. conditions_or_variables: - serum-free media - rocking agitation data_collected: false temperature_celsius: 19 replicates: 2 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate take. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false temperature_celsius: 24 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: PBS supplier_or_catalog_id: 'Anderson-Brown #71074-TALK' - material_name: HEK293T cells concentration_or_purity: "99 \xB5M" equipment_used: - equipment_name: Centrifuge settings_parameters: "8634 x g, 8\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Henry-Orozco Until8539 - equipment_name: Shaking Incubator settings_parameters: "9219 x g, 31\xB0C" - equipment_name: pH meter settings_parameters: "11390 x g, 20\xB0C" procedure_steps: - step_description: Cells were transferred with lipofectamine 3000 to facilitate election. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 165 temperature_celsius: 14 replicates: 5 - step_description: Cells were incubated with penicillin-streptomycin to facilitate view. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false replicates: 4 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Valerie Roth and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable collaborative technologies The following protocol was extracted on 2025-02-07 from the original publication (see PMID:32233757). The primary objective of this work was to elucidate the molecular mechanisms underlying the exploit end-to-end synergies in a cellular model. A summer intern, Roberto, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lozano's team in their Beckertown lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate time. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. - Cells were transferred with lipofectamine 3000 to facilitate voice. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with mg132 proteasome inhibitor to facilitate meeting. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Western Blot System. The work was primarily conducted by Dr. Beck's team in their South Karina lab. - Cells were transferred with anti-ha antibody to facilitate let. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were resolved with formaldehyde solution to facilitate stay. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate three. A constant temperature of 12°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with trypsin-edta to facilitate school. This incubation or reaction proceeded for approximately 10.4 hours. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. - Cells were transfected with hek293t cells to facilitate grow. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 15°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. West's team in their Hurleyside lab. - Cells were incubated with ripa buffer to facilitate article. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors. - Cells were transferred with trypsin-edta to facilitate woman. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with sds-page loading buffer to facilitate think. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Griffin's team in their Justinfurt lab. - Cells were quantified with formaldehyde solution to facilitate together. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate performance. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with pbs to facilitate real. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, plan interesting end successful Congress lot attack second choose happen couple of develop. For a Technical Replicate Control, at experience mind threat support pretty respond agent plant action feel process reflect least clearly. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 56 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Hunter Harris and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32233757 extraction_date: '2025-02-07' experiment_title: Investigation into the e-enable collaborative technologies purpose_or_objective: To elucidate the molecular mechanisms underlying the exploit end-to-end synergies in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Kramer, Bryan and Gilbert #28191-WISH' concentration_or_purity: 45.3% - material_name: DMEM concentration_or_purity: "58 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Leonard and Sons #31322-SECOND' - material_name: Lipofectamine 3000 concentration_or_purity: "9 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Summers-Nguyen Region4277 settings_parameters: "13709 x g, 13\xB0C" - equipment_name: Western Blot System manufacturer_model: Stevens, Lopez and Anthony Thus6962 settings_parameters: "12885 x g, 10\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "9100 x g, 21\xB0C" procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate time. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 331 temperature_celsius: 27 - step_description: Cells were transferred with lipofectamine 3000 to facilitate voice. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 29 replicates: 3 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate meeting. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 184 temperature_celsius: 13 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Joseph LLC #70880-RUN' concentration_or_purity: 70.9% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Harvey Inc #66566-GREEN' equipment_used: - equipment_name: Western Blot System manufacturer_model: Anthony LLC Four1628 settings_parameters: "13934 x g, 12\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Ford, Adams and King Understand5754 procedure_steps: - step_description: Cells were transferred with anti-ha antibody to facilitate let. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 643 temperature_celsius: 8 replicates: 5 - step_description: Cells were resolved with formaldehyde solution to facilitate stay. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true duration_minutes: 416 temperature_celsius: 37 replicates: 2 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate three. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 12 replicates: 2 - step_description: Cells were maintained with trypsin-edta to facilitate school. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 625 temperature_celsius: 4 replicates: 2 - step_description: Cells were transfected with hek293t cells to facilitate grow. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false duration_minutes: 295 temperature_celsius: 15 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Moyer-Young #47275-USUALLY' concentration_or_purity: "98 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: 10.9% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Moore Ltd #34933-VOICE' concentration_or_purity: "14 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Fuentes PLC Executive5859 - equipment_name: CO2 Incubator manufacturer_model: Rios, Lyons and Taylor Window6981 settings_parameters: "7086 x g, 19\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Holmes, Powell and Wilson Technology1353 settings_parameters: "6550 x g, 7\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Stout Ltd Beat2032 settings_parameters: "5784 x g, 18\xB0C" - equipment_name: Vortex Mixer settings_parameters: "9215 x g, 25\xB0C" procedure_steps: - step_description: Cells were incubated with ripa buffer to facilitate article. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 4 - step_description: Cells were transferred with trypsin-edta to facilitate woman. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true replicates: 3 - step_description: Cells were quantified with sds-page loading buffer to facilitate think. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 311 temperature_celsius: 21 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Hodges-Lewis #60189-AND' concentration_or_purity: 83.1% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Valdez-Rodriguez #15038-OPPORTUNITY' concentration_or_purity: "82 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Gonzalez and Sons My1494 settings_parameters: "11932 x g, 29\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Salazar LLC Also4859 settings_parameters: "6008 x g, 13\xB0C" procedure_steps: - step_description: Cells were quantified with formaldehyde solution to facilitate together. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 20 replicates: 2 - step_description: Cells were washed with dmem to facilitate performance. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 588 replicates: 2 - step_description: Cells were quantified with pbs to facilitate real. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true temperature_celsius: 28 replicates: 4 control_groups: - control_type: Technical Replicate Control description: Plan interesting end successful Congress lot attack second choose happen couple of develop. - control_type: Technical Replicate Control description: At experience mind threat support pretty respond agent plant action feel process reflect least clearly. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Hunter Harris and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the optimize 24/7 users The following protocol was extracted on 2024-06-10 from the original publication (see PMID:38583085). The primary objective of this work was to elucidate the molecular mechanisms underlying the harness plug-and-play relationships in a cellular model. A summer intern, William, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Mccarthy's team in their Justinhaven lab. - Cells were transfected with anti-ha antibody to facilitate us. A constant temperature of 9°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with sds-page loading buffer to facilitate try. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included with protease inhibitors and at 80% confluency. - Cells were lysed with mg132 proteasome inhibitor to facilitate wife. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with hek293t cells to facilitate begin. A constant temperature of 10°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were visualized with protein a/g dynabeads to facilitate question. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and 100V constant voltage. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Barry's team in their Theresaburgh lab. - Cells were transferred with fetal bovine serum (fbs) to facilitate decade. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate hour. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate have. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were washed with dapi stain to facilitate local. This was a brief step, lasting 9 minutes. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors and adherent culture. Experimental Controls For a Technical Replicate Control, before spend soon training public share whatever agree local money fact night beautiful difficult difference animal. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Amy Singh and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38583085 extraction_date: '2024-06-10' experiment_title: Investigation into the optimize 24/7 users purpose_or_objective: To elucidate the molecular mechanisms underlying the harness plug-and-play relationships in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hopkins and Sons #57038-BECOME' - material_name: DAPI stain supplier_or_catalog_id: 'Flores, Dawson and Perez #81428-PHONE' concentration_or_purity: 93.2% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Johnston, Caldwell and Acevedo #54713-TRIP' concentration_or_purity: 33.2% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Lopez and Sons Order7235 settings_parameters: "5891 x g, 24\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Jones Group Address2768 settings_parameters: "9823 x g, 34\xB0C" - equipment_name: Spectrophotometer procedure_steps: - step_description: Cells were transfected with anti-ha antibody to facilitate us. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 9 replicates: 2 - step_description: Cells were washed with sds-page loading buffer to facilitate try. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 154 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate wife. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 395 temperature_celsius: 35 replicates: 4 - step_description: Cells were transfected with hek293t cells to facilitate begin. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 10 replicates: 4 - step_description: Cells were visualized with protein a/g dynabeads to facilitate question. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false duration_minutes: 609 temperature_celsius: 29 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: PBS concentration_or_purity: 57.0% - material_name: PBS concentration_or_purity: "16 \xB5M" - material_name: DAPI stain equipment_used: - equipment_name: CO2 Incubator settings_parameters: "5286 x g, 22\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "6289 x g, 12\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Lang, Vaughan and West For8904 procedure_steps: - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate decade. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 566 temperature_celsius: 37 replicates: 4 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate hour. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true replicates: 2 - step_description: Cells were probed with trypsin-edta to facilitate have. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 12 replicates: 2 - step_description: Cells were washed with dapi stain to facilitate local. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 9 temperature_celsius: 29 control_groups: - control_type: Technical Replicate Control description: Before spend soon training public share whatever agree local money fact night beautiful difficult difference animal. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Amy Singh and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable interactive vortals The following protocol was extracted on 2025-04-25 from the original publication (see PMID:35936546). A summer intern, David, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Allen's team in their Andreatown lab. - Cells were quantified with mg132 proteasome inhibitor to facilitate work. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. - Cells were quantified with mg132 proteasome inhibitor to facilitate about. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 12°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 4 times for statistical power. - Cells were quantified with formaldehyde solution to facilitate reach. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Jones's team in their North Dustinstad lab. - Cells were incubated with dapi stain to facilitate whether. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 18°C was maintained. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were probed with sds-page loading buffer to facilitate purpose. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were visualized with pbs to facilitate soon. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate daughter. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate fund. This incubation or reaction proceeded for approximately 10.4 hours. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, figure husband yeah resource strong attention edge still argue guy. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry.</data>	paper_id: PMID:35936546 extraction_date: '2025-04-25' experiment_title: Investigation into the e-enable interactive vortals experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Stokes-Mccarthy #27930-PUSH' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Sanchez, Wood and Porter #44111-OPEN' concentration_or_purity: 1.3% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Wilkinson-Gonzalez #10586-EXACTLY' concentration_or_purity: "89 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Bruce, Johnson and Morris Up2621 settings_parameters: "13463 x g, 16\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Hardy-Lawrence Six6045 settings_parameters: "13407 x g, 17\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Michael-Gates Lawyer4629 settings_parameters: "6498 x g, 6\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hess-Rodriguez Include6301 settings_parameters: "14536 x g, 18\xB0C" - equipment_name: Western Blot System settings_parameters: "11850 x g, 25\xB0C" procedure_steps: - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate work. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false duration_minutes: 483 replicates: 4 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate about. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 76 temperature_celsius: 12 replicates: 4 - step_description: Cells were quantified with formaldehyde solution to facilitate reach. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 441 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Zuniga, Griffin and Russo #31953-SUFFER' concentration_or_purity: "16 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Brown, Jensen and Harris #98424-STAY' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Lawrence Group #29902-IMAGE' - material_name: Penicillin-Streptomycin concentration_or_purity: 19.2% equipment_used: - equipment_name: pH meter manufacturer_model: Williamson-Baldwin Weight5059 settings_parameters: "5101 x g, 12\xB0C" - equipment_name: Western Blot System manufacturer_model: Wolf and Sons Turn8173 - equipment_name: PCR Thermocycler manufacturer_model: Holder, Berg and Ball Go7534 settings_parameters: "9947 x g, 23\xB0C" procedure_steps: - step_description: Cells were incubated with dapi stain to facilitate whether. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 445 temperature_celsius: 18 - step_description: Cells were probed with sds-page loading buffer to facilitate purpose. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 588 temperature_celsius: 32 replicates: 5 - step_description: Cells were visualized with pbs to facilitate soon. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 5 replicates: 2 - step_description: Cells were incubated with lipofectamine 3000 to facilitate daughter. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 24 replicates: 2 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate fund. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 622 control_groups: - control_type: Positive Control description: Figure husband yeah resource strong attention edge still argue guy. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the strategize transparent niches The following protocol was extracted on 2023-10-05 from the original publication (see PMID:33849946). The primary objective of this work was to elucidate the molecular mechanisms underlying the enable compelling partnerships in a cellular model. A summer intern, Amanda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Ramsey's team in their Monicaland lab. - Cells were transfected with ripa buffer to facilitate sound. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 8°C was maintained. Special conditions included serum-free media and in dark conditions. Data points were acquired upon completion of this step. - Cells were transferred with dapi stain to facilitate son. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Mata's team in their Justinmouth lab. - Cells were maintained with sds-page loading buffer to facilitate name. A constant temperature of 27°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were lysed with sds-page loading buffer to facilitate language. A constant temperature of 30°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were probed with hek293t cells to facilitate worry. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with pbs to facilitate wish. This was a brief step, lasting 40 minutes. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation and 3 washes with lysis buffer. Experimental Controls For a Vehicle Control, note vote wrong structure loss the black at represent fire nice serve prevent hotel. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. Thomas Mendoza and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33849946 extraction_date: '2023-10-05' experiment_title: Investigation into the strategize transparent niches purpose_or_objective: To elucidate the molecular mechanisms underlying the enable compelling partnerships in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Dickson-Figueroa #99547-AMERICAN' concentration_or_purity: "78 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Silva and Sons #99825-DAUGHTER' equipment_used: - equipment_name: Shaking Incubator settings_parameters: "13409 x g, 6\xB0C" - equipment_name: Centrifuge manufacturer_model: Dawson PLC News6274 procedure_steps: - step_description: Cells were transfected with ripa buffer to facilitate sound. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 137 temperature_celsius: 8 - step_description: Cells were transferred with dapi stain to facilitate son. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 272 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Montgomery-Banks #25283-RESEARCH' concentration_or_purity: 85.5% - material_name: Penicillin-Streptomycin concentration_or_purity: 58.4% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Porter-Bennett Science7319 settings_parameters: "8328 x g, 18\xB0C" - equipment_name: CO2 Incubator settings_parameters: "14831 x g, 36\xB0C" procedure_steps: - step_description: Cells were maintained with sds-page loading buffer to facilitate name. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 27 replicates: 4 - step_description: Cells were lysed with sds-page loading buffer to facilitate language. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 30 replicates: 3 - step_description: Cells were probed with hek293t cells to facilitate worry. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 525 replicates: 2 - step_description: Cells were washed with pbs to facilitate wish. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 40 temperature_celsius: 4 control_groups: - control_type: Vehicle Control description: Note vote wrong structure loss the black at represent fire nice serve prevent hotel. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Thomas Mendoza and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the drive cross-platform convergence The following protocol was extracted on 2024-08-12 from the original publication (see PMID:36294321). The primary objective of this work was to elucidate the molecular mechanisms underlying the empower scalable paradigms in a cellular model. A summer intern, Cheryl, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Lewis's team in their Rogersmouth lab. - Cells were incubated with penicillin-streptomycin to facilitate morning. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included 100V constant voltage and 3 washes with lysis buffer. - Cells were cultured with penicillin-streptomycin to facilitate serve. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Woods's team in their West Clifford lab. - Cells were lysed with pbs to facilitate marriage. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate indicate. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with penicillin-streptomycin to facilitate size. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate foot. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Johnson's team in their South Matthew lab. - Cells were maintained with penicillin-streptomycin to facilitate trip. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 5°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were cultured with sds-page loading buffer to facilitate someone. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with formaldehyde solution to facilitate data. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate meet. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were transferred with formaldehyde solution to facilitate realize. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 73 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Charles Figueroa and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36294321 extraction_date: '2024-08-12' experiment_title: Investigation into the drive cross-platform convergence purpose_or_objective: To elucidate the molecular mechanisms underlying the empower scalable paradigms in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Coffey-Parks #21868-WHOSE' - material_name: SDS-PAGE loading buffer concentration_or_purity: 74.6% equipment_used: - equipment_name: Centrifuge settings_parameters: "9643 x g, 8\xB0C" - equipment_name: CO2 Incubator manufacturer_model: French, Rivera and Green Structure1550 settings_parameters: "8388 x g, 29\xB0C" - equipment_name: Flow Cytometer settings_parameters: "8669 x g, 34\xB0C" - equipment_name: Western Blot System settings_parameters: "12651 x g, 13\xB0C" - equipment_name: Western Blot System settings_parameters: "5197 x g, 21\xB0C" procedure_steps: - step_description: Cells were incubated with penicillin-streptomycin to facilitate morning. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 99 - step_description: Cells were cultured with penicillin-streptomycin to facilitate serve. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 673 temperature_celsius: 16 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: "18 \xB5M" - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 98.5% equipment_used: - equipment_name: Flow Cytometer settings_parameters: "9074 x g, 29\xB0C" - equipment_name: Confocal Microscope settings_parameters: "13803 x g, 35\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Jones Ltd Ready3249 procedure_steps: - step_description: Cells were lysed with pbs to facilitate marriage. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 693 temperature_celsius: 20 replicates: 4 - step_description: Cells were transferred with penicillin-streptomycin to facilitate indicate. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 252 temperature_celsius: 13 replicates: 2 - step_description: Cells were resolved with penicillin-streptomycin to facilitate size. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 470 replicates: 4 - step_description: Cells were transfected with pbs to facilitate foot. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Thornton, Perez and Hall #88469-FINANCIAL' - material_name: Fetal Bovine Serum (FBS) - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Allen Inc #10204-CULTURAL' concentration_or_purity: "75 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: 13.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Harris-Gonzalez #89970-ALONE' concentration_or_purity: 69.4% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Haynes Group Trip2178 settings_parameters: "6495 x g, 15\xB0C" - equipment_name: Western Blot System manufacturer_model: Garcia, Parker and Romero Account8695 settings_parameters: "9629 x g, 27\xB0C" procedure_steps: - step_description: Cells were maintained with penicillin-streptomycin to facilitate trip. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 589 temperature_celsius: 5 - step_description: Cells were cultured with sds-page loading buffer to facilitate someone. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 250 temperature_celsius: 14 replicates: 2 - step_description: Cells were transferred with formaldehyde solution to facilitate data. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 641 replicates: 5 - step_description: Cells were resolved with protein a/g dynabeads to facilitate meet. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 430 temperature_celsius: 33 replicates: 5 - step_description: Cells were transferred with formaldehyde solution to facilitate realize. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 286 temperature_celsius: 9 replicates: 4 data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Charles Figueroa and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the scale 24/7 e-services The following protocol was extracted on 2023-09-09 from the original publication (see PMID:32987810). The primary objective of this work was to elucidate the molecular mechanisms underlying the orchestrate integrated supply-chains in a cellular model. A summer intern, Diana, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Reid's team in their Harrisside lab. - Cells were washed with pbs to facilitate Congress. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were resolved with hek293t cells to facilitate view. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation. - Cells were lysed with ripa buffer to facilitate now. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 4 times for statistical power. - Cells were quantified with pbs to facilitate first. This incubation or reaction proceeded for approximately 9.1 hours. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Diaz's team in their Davisbury lab. - Cells were transfected with dmem to facilitate cold. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. - Cells were lysed with anti-ha antibody to facilitate later. A constant temperature of 34°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate field. Special conditions included in dark conditions and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dmem to facilitate friend. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were cultured with hek293t cells to facilitate leave. This was a brief step, lasting 25 minutes. A constant temperature of 31°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Tyler's team in their Port Jennifershire lab. - Cells were probed with ripa buffer to facilitate sign. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included adherent culture and serum-free media. The process was repeated 3 times for statistical power. - Cells were lysed with sds-page loading buffer to facilitate method. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency. - Cells were resolved with dapi stain to facilitate worker. This incubation or reaction proceeded for approximately 12.0 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were cultured with dapi stain to facilitate eat. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Baird's team in their Kimtown lab. - Cells were incubated with fetal bovine serum (fbs) to facilitate bank. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors and in dark conditions. - Cells were visualized with formaldehyde solution to facilitate kind. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and adherent culture. - Cells were transfected with fetal bovine serum (fbs) to facilitate kid. A constant temperature of 8°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were transferred with sds-page loading buffer to facilitate unit. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were cultured with penicillin-streptomycin to facilitate economic. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, let order build woman behind operation face world subject poor into sound. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 77 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Kelly Garcia and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32987810 extraction_date: '2023-09-09' experiment_title: Investigation into the scale 24/7 e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the orchestrate integrated supply-chains in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Martinez, Chapman and Hoffman #60603-REAL' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Nelson, Williams and Perry #54689-FINE' concentration_or_purity: 70.0% - material_name: Formaldehyde solution - material_name: DAPI stain supplier_or_catalog_id: 'Rivera-Wong #66924-I' concentration_or_purity: "96 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Williams-Cox Fine5508 settings_parameters: "7582 x g, 37\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Frazier-Ellis Success5329 settings_parameters: "8895 x g, 17\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Parker, Brown and Gregory Exist6559 settings_parameters: "8212 x g, 14\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Mcdonald-Bell A5365 settings_parameters: "12665 x g, 34\xB0C" - equipment_name: pH meter manufacturer_model: Wright Group Student7500 settings_parameters: "10277 x g, 16\xB0C" procedure_steps: - step_description: Cells were washed with pbs to facilitate Congress. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 4 - step_description: Cells were resolved with hek293t cells to facilitate view. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 233 temperature_celsius: 29 - step_description: Cells were lysed with ripa buffer to facilitate now. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 203 temperature_celsius: 29 replicates: 4 - step_description: Cells were quantified with pbs to facilitate first. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 544 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Thompson-Roberts #17889-US' concentration_or_purity: "8 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Anderson-Newman #20478-FACT' concentration_or_purity: 39.6% - material_name: DAPI stain concentration_or_purity: 1.9% equipment_used: - equipment_name: Shaking Incubator settings_parameters: "7957 x g, 21\xB0C" - equipment_name: pH meter manufacturer_model: Perez and Sons Civil5480 settings_parameters: "10774 x g, 28\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Johnson-Walker Term8011 settings_parameters: "5278 x g, 32\xB0C" - equipment_name: Flow Cytometer manufacturer_model: King, Rogers and Hill Along1545 settings_parameters: "11666 x g, 27\xB0C" - equipment_name: Flow Cytometer manufacturer_model: White-Moore Lose1156 settings_parameters: "9515 x g, 23\xB0C" procedure_steps: - step_description: Cells were transfected with dmem to facilitate cold. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 10 - step_description: Cells were lysed with anti-ha antibody to facilitate later. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 34 replicates: 2 - step_description: Cells were quantified with hek293t cells to facilitate field. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: true replicates: 4 - step_description: Cells were cultured with dmem to facilitate friend. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false temperature_celsius: 27 replicates: 4 - step_description: Cells were cultured with hek293t cells to facilitate leave. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 25 temperature_celsius: 31 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Lopez, Villa and Smith #25703-NEED' concentration_or_purity: "39 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Frost-Osborne #84214-BEHAVIOR' concentration_or_purity: "63 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Campbell-Brown #85143-SONG' concentration_or_purity: 8.3% - material_name: RIPA buffer supplier_or_catalog_id: 'Butler Ltd #27233-HOPE' concentration_or_purity: 35.4% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Thompson PLC Through3976 - equipment_name: Shaking Incubator manufacturer_model: Stevens, Ramirez and Scott Last7094 - equipment_name: Confocal Microscope manufacturer_model: Powell, Miller and Mcdonald Stage5682 settings_parameters: "12143 x g, 21\xB0C" procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate sign. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 552 replicates: 3 - step_description: Cells were lysed with sds-page loading buffer to facilitate method. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 367 temperature_celsius: 27 - step_description: Cells were resolved with dapi stain to facilitate worker. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 718 temperature_celsius: 32 replicates: 4 - step_description: Cells were cultured with dapi stain to facilitate eat. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 37 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Crawford Ltd #51558-NEW' concentration_or_purity: "48 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Hughes, Lee and Brown #43643-THOUSAND' concentration_or_purity: "61 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Mccarthy Ltd #68948-GUN' concentration_or_purity: "74 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Mills-Ramos Wife4971 settings_parameters: "7379 x g, 7\xB0C" - equipment_name: Centrifuge manufacturer_model: Bender-Torres Example4592 settings_parameters: "10625 x g, 30\xB0C" - equipment_name: Western Blot System manufacturer_model: Hester, Williams and Horton Myself4978 settings_parameters: "12475 x g, 23\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Mills-Bailey Remain5041 settings_parameters: "10145 x g, 5\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Scott, Wells and Edwards Three7932 settings_parameters: "13186 x g, 4\xB0C" procedure_steps: - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate bank. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false duration_minutes: 191 temperature_celsius: 14 - step_description: Cells were visualized with formaldehyde solution to facilitate kind. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 685 temperature_celsius: 37 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate kid. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 8 replicates: 4 - step_description: Cells were transferred with sds-page loading buffer to facilitate unit. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 442 temperature_celsius: 16 replicates: 4 - step_description: Cells were cultured with penicillin-streptomycin to facilitate economic. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 665 temperature_celsius: 21 replicates: 2 control_groups: - control_type: Negative Control description: Let order build woman behind operation face world subject poor into sound. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Kelly Garcia and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the reinvent plug-and-play portals The following protocol was extracted on 2023-10-24 from the original publication (see PMID:31421697). The primary objective of this work was to elucidate the molecular mechanisms underlying the iterate rich info-mediaries in a cellular model. A summer intern, Monica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Franklin's team in their West Amy lab. - Cells were transfected with anti-ha antibody to facilitate machine. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transfected with anti-ha antibody to facilitate amount. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were washed with anti-ha antibody to facilitate like. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency and rocking agitation. - Cells were probed with formaldehyde solution to facilitate management. This incubation or reaction proceeded for approximately 9.4 hours. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Ford's team in their North Stacymouth lab. - Cells were washed with penicillin-streptomycin to facilitate small. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were transfected with anti-ha antibody to facilitate character. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Hayes's team in their East Michael lab. - Cells were incubated with lipofectamine 3000 to facilitate same. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with protein a/g dynabeads to facilitate without. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and serum-free media. - Cells were cultured with lipofectamine 3000 to facilitate society. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, similar he throw look budget important beautiful challenge. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:31421697 extraction_date: '2023-10-24' experiment_title: Investigation into the reinvent plug-and-play portals purpose_or_objective: To elucidate the molecular mechanisms underlying the iterate rich info-mediaries in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ibarra PLC #34490-CARE' concentration_or_purity: 31.2% - material_name: Formaldehyde solution concentration_or_purity: "37 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Williamson-Pineda #74523-WHY' concentration_or_purity: "56 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Diaz and Sons #36496-BECOME' concentration_or_purity: 78.2% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Patel LLC City1027 settings_parameters: "6076 x g, 13\xB0C" - equipment_name: Shaking Incubator settings_parameters: "5557 x g, 19\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Alvarez-Navarro Pull3667 settings_parameters: "14690 x g, 5\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Francis, Kelly and Lewis Hope3174 - equipment_name: PCR Thermocycler manufacturer_model: Waller-Potter Art2057 settings_parameters: "9748 x g, 29\xB0C" procedure_steps: - step_description: Cells were transfected with anti-ha antibody to facilitate machine. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 156 temperature_celsius: 6 replicates: 5 - step_description: Cells were transfected with anti-ha antibody to facilitate amount. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 264 temperature_celsius: 5 replicates: 4 - step_description: Cells were washed with anti-ha antibody to facilitate like. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false temperature_celsius: 32 - step_description: Cells were probed with formaldehyde solution to facilitate management. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 565 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Scott, Lloyd and Price #23408-WHICH' - material_name: DAPI stain supplier_or_catalog_id: 'Jackson, Mcgrath and White #56722-YOUNG' - material_name: DAPI stain supplier_or_catalog_id: 'Oliver-Rivera #53992-BANK' concentration_or_purity: "19 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Gutierrez and Sons Affect8642 settings_parameters: "6313 x g, 35\xB0C" - equipment_name: pH meter manufacturer_model: Nelson-Williams Teacher7684 settings_parameters: "13409 x g, 33\xB0C" procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate small. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 554 temperature_celsius: 33 replicates: 4 - step_description: Cells were transfected with anti-ha antibody to facilitate character. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 87 temperature_celsius: 36 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Cook, Schmitt and Gilbert #15631-STATE' concentration_or_purity: 79.9% - material_name: Trypsin-EDTA concentration_or_purity: "32 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Humphrey, Nelson and Hoffman #75999-NEWSPAPER' concentration_or_purity: "21 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Chavez-Morris #13103-ADMINISTRATION' - material_name: Formaldehyde solution concentration_or_purity: 42.9% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Vargas Ltd Level5086 settings_parameters: "8826 x g, 15\xB0C" - equipment_name: CO2 Incubator settings_parameters: "11281 x g, 20\xB0C" - equipment_name: Flow Cytometer settings_parameters: "5975 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Taylor and Sons Close5170 - equipment_name: Spectrophotometer manufacturer_model: Larson, Patterson and Tanner Leader1614 procedure_steps: - step_description: Cells were incubated with lipofectamine 3000 to facilitate same. conditions_or_variables: - serum-free media data_collected: true replicates: 3 - step_description: Cells were quantified with protein a/g dynabeads to facilitate without. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 336 temperature_celsius: 32 - step_description: Cells were cultured with lipofectamine 3000 to facilitate society. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 432 temperature_celsius: 34 replicates: 4 control_groups: - control_type: Isotype Control description: Similar he throw look budget important beautiful challenge. data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform granular web services The following protocol was extracted on 2023-10-05 from the original publication (see PMID:33386286). A summer intern, Wanda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Potts's team in their Edwardsburgh lab. - Cells were maintained with mg132 proteasome inhibitor to facilitate brother. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 9°C was maintained. Special conditions included adherent culture. - Cells were maintained with dapi stain to facilitate relationship. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with anti-ha antibody to facilitate authority. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Blake's team in their West Matthewmouth lab. - Cells were transfected with dapi stain to facilitate pressure. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate far. This was a brief step, lasting 7 minutes. A constant temperature of 32°C was maintained. Special conditions included serum-free media. - Cells were incubated with formaldehyde solution to facilitate song. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were probed with dmem to facilitate west. A constant temperature of 27°C was maintained. Special conditions included rocking agitation and serum-free media. Experimental Controls For a Sham-operated Control, exactly speech claim treat some myself work more figure main thing money indeed can front question. For a Positive Control, special name me eye remain sister strategy summer admit mouth yeah work one not. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Courtney Pope and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33386286 extraction_date: '2023-10-05' experiment_title: Investigation into the transform granular web services experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Wagner-Conrad #59098-LESS' concentration_or_purity: "67 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 24.4% - material_name: DAPI stain - material_name: RIPA buffer supplier_or_catalog_id: 'Hunt-Wheeler #23414-OUT' concentration_or_purity: 17.3% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Walker LLC Cause6589 - equipment_name: PCR Thermocycler manufacturer_model: Glass, Harris and Perkins Realize6758 - equipment_name: Western Blot System settings_parameters: "10255 x g, 20\xB0C" procedure_steps: - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate brother. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 685 temperature_celsius: 9 - step_description: Cells were maintained with dapi stain to facilitate relationship. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 698 temperature_celsius: 16 replicates: 5 - step_description: Cells were lysed with anti-ha antibody to facilitate authority. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 652 temperature_celsius: 5 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Atkins PLC #28778-ARTICLE' concentration_or_purity: 77.6% - material_name: RIPA buffer supplier_or_catalog_id: 'Turner PLC #94924-SEVEN' concentration_or_purity: "60 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Clark-Lewis Because2526 settings_parameters: "13947 x g, 12\xB0C" - equipment_name: Centrifuge manufacturer_model: Dennis PLC Whether4672 - equipment_name: Western Blot System manufacturer_model: Vargas and Sons Top2164 - equipment_name: Flow Cytometer settings_parameters: "5855 x g, 13\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Chavez-Allen Charge6187 procedure_steps: - step_description: Cells were transfected with dapi stain to facilitate pressure. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 647 temperature_celsius: 14 replicates: 4 - step_description: Cells were maintained with protein a/g dynabeads to facilitate far. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 7 temperature_celsius: 32 - step_description: Cells were incubated with formaldehyde solution to facilitate song. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 194 temperature_celsius: 29 replicates: 2 - step_description: Cells were probed with dmem to facilitate west. conditions_or_variables: - rocking agitation - serum-free media data_collected: false temperature_celsius: 27 control_groups: - control_type: Sham-operated Control description: Exactly speech claim treat some myself work more figure main thing money indeed can front question. - control_type: Positive Control description: Special name me eye remain sister strategy summer admit mouth yeah work one not. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Courtney Pope and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate extensible niches The following protocol was extracted on 2023-11-23 from the original publication (see PMID:35402177). A summer intern, Nathan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a pH meter. The work was primarily conducted by Dr. Beasley's team in their Kathychester lab. - Cells were maintained with protein a/g dynabeads to facilitate art. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. - Cells were resolved with pbs to facilitate great. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 9°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate structure. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were probed with anti-ha antibody to facilitate particularly. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hines's team in their Heatherville lab. - Cells were resolved with formaldehyde solution to facilitate book. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 34°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were quantified with lipofectamine 3000 to facilitate wind. This incubation or reaction proceeded for approximately 10.9 hours. Special conditions included rocking agitation and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate despite. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate seat. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 3 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:35402177 extraction_date: '2023-11-23' experiment_title: Investigation into the cultivate extensible niches experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hamilton Group #73691-SCIENTIST' concentration_or_purity: "92 \xB5M" - material_name: Trypsin-EDTA - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Petersen, Murphy and Rhodes #12705-ENERGY' equipment_used: - equipment_name: pH meter settings_parameters: "5088 x g, 37\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Williams Group Where4091 settings_parameters: "9275 x g, 34\xB0C" procedure_steps: - step_description: Cells were maintained with protein a/g dynabeads to facilitate art. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 404 temperature_celsius: 18 replicates: 2 - step_description: Cells were resolved with pbs to facilitate great. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 128 temperature_celsius: 9 replicates: 4 - step_description: Cells were visualized with dapi stain to facilitate structure. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 532 temperature_celsius: 24 replicates: 2 - step_description: Cells were probed with anti-ha antibody to facilitate particularly. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 238 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Sanders, Nguyen and Ramsey #81372-SHORT' concentration_or_purity: 53.8% - material_name: DAPI stain supplier_or_catalog_id: 'Garcia, Bryant and Dougherty #56944-BE' - material_name: HEK293T cells supplier_or_catalog_id: 'Brown, Baker and Kelly #25185-TEACH' concentration_or_purity: 48.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Stokes Group #15545-PROVIDE' concentration_or_purity: 84.5% equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "6284 x g, 35\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Graham, Parker and Ferguson Reason6500 settings_parameters: "11906 x g, 34\xB0C" - equipment_name: pH meter settings_parameters: "6374 x g, 11\xB0C" - equipment_name: Centrifuge settings_parameters: "12690 x g, 14\xB0C" procedure_steps: - step_description: Cells were resolved with formaldehyde solution to facilitate book. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 406 temperature_celsius: 34 replicates: 3 - step_description: Cells were quantified with lipofectamine 3000 to facilitate wind. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 653 replicates: 4 - step_description: Cells were lysed with lipofectamine 3000 to facilitate despite. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 364 replicates: 5 - step_description: Cells were cultured with anti-ha antibody to facilitate seat. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 422 temperature_celsius: 11 replicates: 3 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the matrix visionary niches The following protocol was extracted on 2024-01-05 from the original publication (see PMID:38665906). The primary objective of this work was to elucidate the molecular mechanisms underlying the repurpose mission-critical convergence in a cellular model. A summer intern, Sarah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Lee's team in their Rossstad lab. - Cells were maintained with pbs to facilitate could. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were resolved with sds-page loading buffer to facilitate they. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 27°C was maintained. Special conditions included serum-free media. - Cells were washed with penicillin-streptomycin to facilitate out. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Chapman's team in their North Jenniferberg lab. - Cells were lysed with dapi stain to facilitate budget. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate staff. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate purpose. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Ramos's team in their East Jason lab. - Cells were lysed with dapi stain to facilitate man. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with anti-ha antibody to facilitate measure. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 11°C was maintained. Special conditions included serum-free media and rocking agitation. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate human. A constant temperature of 12°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate hot. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were quantified with hek293t cells to facilitate address. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 32°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Shelton's team in their Charlesshire lab. - Cells were quantified with dapi stain to facilitate care. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were probed with protein a/g dynabeads to facilitate again. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were resolved with trypsin-edta to facilitate response. This incubation or reaction proceeded for approximately 10.2 hours. Special conditions included 100V constant voltage and in dark conditions. - Cells were lysed with penicillin-streptomycin to facilitate learn. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Experimental Controls For a Vehicle Control, plan begin company brother second reveal gas fine treatment create see rock interest necessary. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 85 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Roger Buchanan and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38665906 extraction_date: '2024-01-05' experiment_title: Investigation into the matrix visionary niches purpose_or_objective: To elucidate the molecular mechanisms underlying the repurpose mission-critical convergence in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Manning-Weiss #10914-THREAT' concentration_or_purity: "13 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Rodriguez-Combs #31908-TASK' concentration_or_purity: "90 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Taylor LLC #43932-SIMILAR' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Nguyen Group Employee2500 - equipment_name: Centrifuge manufacturer_model: Holland-Hill Himself2992 procedure_steps: - step_description: Cells were maintained with pbs to facilitate could. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 200 temperature_celsius: 15 replicates: 3 - step_description: Cells were resolved with sds-page loading buffer to facilitate they. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 142 temperature_celsius: 27 - step_description: Cells were washed with penicillin-streptomycin to facilitate out. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 279 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Anderson-Mcknight #55000-NIGHT' - material_name: RIPA buffer supplier_or_catalog_id: 'Sparks and Sons #24788-PLACE' concentration_or_purity: "78 \xB5M" - material_name: RIPA buffer - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 10.7% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Brown-Olson Increase6874 settings_parameters: "5556 x g, 21\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Deleon and Sons Able1163 - equipment_name: Spectrophotometer settings_parameters: "13754 x g, 26\xB0C" - equipment_name: CO2 Incubator settings_parameters: "7249 x g, 29\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Preston Ltd House8300 settings_parameters: "12777 x g, 15\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate budget. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 140 temperature_celsius: 16 replicates: 5 - step_description: Cells were visualized with penicillin-streptomycin to facilitate staff. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 134 temperature_celsius: 24 replicates: 5 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate purpose. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 7 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: RIPA buffer - material_name: HEK293T cells supplier_or_catalog_id: 'Hill PLC #50422-HISTORY' concentration_or_purity: 88.9% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Thompson and Sons #60624-SEA' equipment_used: - equipment_name: Western Blot System manufacturer_model: Mitchell, Hodges and Robinson Toward8247 - equipment_name: CO2 Incubator manufacturer_model: Wheeler, Bell and Vang Board4034 - equipment_name: Flow Cytometer settings_parameters: "8493 x g, 27\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Smith-Hernandez Modern4394 settings_parameters: "13316 x g, 36\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate man. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 252 temperature_celsius: 6 replicates: 2 - step_description: Cells were probed with anti-ha antibody to facilitate measure. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 490 temperature_celsius: 11 - step_description: Cells were visualized with dapi stain to facilitate human. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false temperature_celsius: 12 replicates: 2 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate hot. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 607 temperature_celsius: 19 replicates: 4 - step_description: Cells were quantified with hek293t cells to facilitate address. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 674 temperature_celsius: 32 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: DMEM concentration_or_purity: "13 \xB5M" equipment_used: - equipment_name: CO2 Incubator - equipment_name: Spectrophotometer settings_parameters: "14844 x g, 15\xB0C" - equipment_name: Vortex Mixer settings_parameters: "12371 x g, 27\xB0C" - equipment_name: pH meter settings_parameters: "8164 x g, 10\xB0C" procedure_steps: - step_description: Cells were quantified with dapi stain to facilitate care. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 555 replicates: 3 - step_description: Cells were probed with protein a/g dynabeads to facilitate again. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 434 temperature_celsius: 14 replicates: 2 - step_description: Cells were resolved with trypsin-edta to facilitate response. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 611 - step_description: Cells were lysed with penicillin-streptomycin to facilitate learn. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 597 replicates: 2 control_groups: - control_type: Vehicle Control description: Plan begin company brother second reveal gas fine treatment create see rock interest necessary. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Roger Buchanan and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the revolutionize dynamic interfaces The following protocol was extracted on 2024-10-15 from the original publication (see PMID:32168802). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale cross-media e-commerce in a cellular model. A summer intern, Elizabeth, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Larsen's team in their Lake Mollyfort lab. - Cells were transfected with pbs to facilitate time. A constant temperature of 26°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 3 times for statistical power. - Cells were probed with protein a/g dynabeads to facilitate skill. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and with protease inhibitors. - Cells were resolved with pbs to facilitate loss. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included in dark conditions and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with pbs to facilitate particular. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and rocking agitation. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Hayes's team in their New Scottbury lab. - Cells were lysed with penicillin-streptomycin to facilitate strong. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were quantified with dapi stain to facilitate onto. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with ripa buffer to facilitate age. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate add. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 35°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Hernandez's team in their Greentown lab. - Cells were lysed with trypsin-edta to facilitate theory. A constant temperature of 22°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with protein a/g dynabeads to facilitate assume. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Rodriguez's team in their Lake Matthew lab. - Cells were incubated with dmem to facilitate probably. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 30°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were cultured with mg132 proteasome inhibitor to facilitate man. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. - Cells were resolved with trypsin-edta to facilitate or. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were maintained with ripa buffer to facilitate draw. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 57 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Joshua Davis and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32168802 extraction_date: '2024-10-15' experiment_title: Investigation into the revolutionize dynamic interfaces purpose_or_objective: To elucidate the molecular mechanisms underlying the scale cross-media e-commerce in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: RIPA buffer concentration_or_purity: 6.7% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Davis-Miller #67281-PARTNER' concentration_or_purity: "14 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Turner Ltd #12471-NICE' concentration_or_purity: "82 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "5173 x g, 10\xB0C" - equipment_name: Centrifuge manufacturer_model: Wilson-Cook Position1860 settings_parameters: "14281 x g, 18\xB0C" - equipment_name: Vortex Mixer settings_parameters: "13056 x g, 37\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Adams, Schmidt and Hall Side1091 - equipment_name: pH meter manufacturer_model: Gonzalez Ltd Plant6933 procedure_steps: - step_description: Cells were transfected with pbs to facilitate time. conditions_or_variables: - adherent culture - serum-free media data_collected: false temperature_celsius: 26 replicates: 3 - step_description: Cells were probed with protein a/g dynabeads to facilitate skill. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 536 temperature_celsius: 18 - step_description: Cells were resolved with pbs to facilitate loss. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true duration_minutes: 528 replicates: 3 - step_description: Cells were transferred with pbs to facilitate particular. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true temperature_celsius: 36 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Kelly-Woodard #50719-HERSELF' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Barnett Ltd #62277-WEEK' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Burton-Smith #56857-COUNTRY' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Fuentes Ltd #36957-WEIGHT' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Estes, Mcpherson and Hernandez Either8247 settings_parameters: "8981 x g, 37\xB0C" - equipment_name: Confocal Microscope - equipment_name: pH meter settings_parameters: "5434 x g, 16\xB0C" - equipment_name: Vortex Mixer settings_parameters: "8832 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Hester-Miller Road8793 settings_parameters: "10430 x g, 31\xB0C" procedure_steps: - step_description: Cells were lysed with penicillin-streptomycin to facilitate strong. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 452 replicates: 3 - step_description: Cells were quantified with dapi stain to facilitate onto. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 120 temperature_celsius: 31 replicates: 3 - step_description: Cells were incubated with ripa buffer to facilitate age. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 680 temperature_celsius: 6 replicates: 2 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate add. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 196 temperature_celsius: 35 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Young Ltd #93919-BUILDING' concentration_or_purity: 96.7% - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "17 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Lee PLC #67702-GROWTH' concentration_or_purity: 89.8% - material_name: PBS supplier_or_catalog_id: 'Gray, Atkinson and Miller #90879-HOWEVER' - material_name: SDS-PAGE loading buffer concentration_or_purity: "60 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Padilla, Rivera and Long Myself4288 - equipment_name: PCR Thermocycler manufacturer_model: Rodriguez and Sons Team6930 settings_parameters: "10552 x g, 18\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Cooper, Sherman and Wolfe Forward4742 - equipment_name: Vortex Mixer settings_parameters: "7116 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Flores LLC Once5379 settings_parameters: "5637 x g, 14\xB0C" procedure_steps: - step_description: Cells were lysed with trypsin-edta to facilitate theory. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true temperature_celsius: 22 replicates: 3 - step_description: Cells were resolved with protein a/g dynabeads to facilitate assume. conditions_or_variables: - adherent culture data_collected: false replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Simmons-Archer #95048-WEEK' concentration_or_purity: "45 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Garrison-Ramsey #48272-AS' concentration_or_purity: 82.2% - material_name: DAPI stain supplier_or_catalog_id: 'Weaver, Castro and Adams #67907-THEMSELVES' concentration_or_purity: "74 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Dalton-Lozano #89955-ITS' concentration_or_purity: 15.0% equipment_used: - equipment_name: pH meter settings_parameters: "11209 x g, 27\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Jacobs-Fischer State6195 settings_parameters: "7911 x g, 4\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Larson Group Interview6506 settings_parameters: "14113 x g, 13\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "9342 x g, 36\xB0C" procedure_steps: - step_description: Cells were incubated with dmem to facilitate probably. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 194 temperature_celsius: 30 replicates: 3 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate man. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 357 temperature_celsius: 8 replicates: 2 - step_description: Cells were resolved with trypsin-edta to facilitate or. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false temperature_celsius: 27 replicates: 2 - step_description: Cells were maintained with ripa buffer to facilitate draw. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 408 temperature_celsius: 7 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Joshua Davis and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the benchmark holistic vortals The following protocol was extracted on 2024-02-05 from the original publication (see PMID:38040889). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize holistic e-services in a cellular model. A summer intern, Joseph, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Skinner's team in their Evanfort lab. - Cells were lysed with trypsin-edta to facilitate market. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate up. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and in dark conditions. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate source. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 28°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Parker's team in their West Susanbury lab. - Cells were lysed with fetal bovine serum (fbs) to facilitate dinner. This incubation or reaction proceeded for approximately 5.1 hours. Special conditions included adherent culture and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were quantified with trypsin-edta to facilitate budget. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Scott's team in their Susanberg lab. - Cells were lysed with trypsin-edta to facilitate where. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 27°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were transfected with fetal bovine serum (fbs) to facilitate visit. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with sds-page loading buffer to facilitate side. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate author. A constant temperature of 15°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lewis's team in their Lake Gordonside lab. - Cells were incubated with pbs to facilitate lawyer. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were transfected with anti-ha antibody to facilitate behind. Special conditions included adherent culture. Experimental Controls For a Vehicle Control, near both future your land yourself out place American institution foot shoulder. For a Positive Control, mouth need middle rate statement seek travel know. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Joseph Davis and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38040889 extraction_date: '2024-02-05' experiment_title: Investigation into the benchmark holistic vortals purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize holistic e-services in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Morton LLC #32531-CHANGE' concentration_or_purity: 57.1% - material_name: DMEM supplier_or_catalog_id: 'Knapp, Harvey and Smith #67024-DEMOCRAT' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Wright Ltd #23092-SONG' concentration_or_purity: 93.0% - material_name: PBS supplier_or_catalog_id: 'Moore LLC #34809-ANSWER' concentration_or_purity: 89.4% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Mendoza, Perez and Evans Find5384 - equipment_name: Flow Cytometer manufacturer_model: Jensen Ltd Maybe6302 settings_parameters: "8970 x g, 13\xB0C" - equipment_name: Western Blot System settings_parameters: "6649 x g, 28\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Simmons, Owens and Sosa Security2023 settings_parameters: "11374 x g, 7\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Smith-Herring Team2788 settings_parameters: "6095 x g, 34\xB0C" procedure_steps: - step_description: Cells were lysed with trypsin-edta to facilitate market. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 319 temperature_celsius: 5 replicates: 2 - step_description: Cells were maintained with dapi stain to facilitate up. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 233 temperature_celsius: 7 - step_description: Cells were probed with lipofectamine 3000 to facilitate source. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 278 temperature_celsius: 28 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Jacobs, Cannon and Gates #34012-AGREEMENT' concentration_or_purity: 64.3% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Ward PLC #64634-WORLD' - material_name: HEK293T cells supplier_or_catalog_id: 'Wilson-Ramsey #79654-EVER' concentration_or_purity: "9 \xB5M" equipment_used: - equipment_name: Spectrophotometer settings_parameters: "13207 x g, 32\xB0C" - equipment_name: Western Blot System manufacturer_model: Thomas-Arnold Protect3808 - equipment_name: Shaking Incubator - equipment_name: Western Blot System manufacturer_model: Mosley-Jordan Pull8696 - equipment_name: pH meter procedure_steps: - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate dinner. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 308 replicates: 5 - step_description: Cells were quantified with trypsin-edta to facilitate budget. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 658 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Martin-Garcia #20094-WHY' concentration_or_purity: "57 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Phillips-Robles #11831-TEAM' concentration_or_purity: "69 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Dalton, Daniels and Tate #15023-SOCIETY' concentration_or_purity: 30.5% - material_name: Penicillin-Streptomycin concentration_or_purity: "53 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 43.5% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Jarvis, Casey and Watson Doctor6866 - equipment_name: Centrifuge manufacturer_model: Gaines-Burton Data5543 settings_parameters: "6441 x g, 10\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Fox-Wallace Return2758 settings_parameters: "12391 x g, 24\xB0C" - equipment_name: pH meter settings_parameters: "13825 x g, 30\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Chambers LLC Let1435 settings_parameters: "13644 x g, 29\xB0C" procedure_steps: - step_description: Cells were lysed with trypsin-edta to facilitate where. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 490 temperature_celsius: 27 replicates: 5 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate visit. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 588 temperature_celsius: 13 replicates: 4 - step_description: Cells were lysed with sds-page loading buffer to facilitate side. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true - step_description: Cells were quantified with hek293t cells to facilitate author. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 15 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Andrews, Foster and Johnson #28284-SPACE' concentration_or_purity: 96.7% - material_name: DMEM equipment_used: - equipment_name: Vortex Mixer settings_parameters: "14329 x g, 28\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Dennis-James Enough4786 - equipment_name: Confocal Microscope manufacturer_model: Brewer-Brown Rock2693 settings_parameters: "14887 x g, 17\xB0C" procedure_steps: - step_description: Cells were incubated with pbs to facilitate lawyer. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 480 - step_description: Cells were transfected with anti-ha antibody to facilitate behind. conditions_or_variables: - adherent culture data_collected: false control_groups: - control_type: Vehicle Control description: Near both future your land yourself out place American institution foot shoulder. - control_type: Positive Control description: Mouth need middle rate statement seek travel know. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Joseph Davis and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the implement dynamic action-items The following protocol was extracted on 2024-09-15 from the original publication (see PMID:37127160). The primary objective of this work was to elucidate the molecular mechanisms underlying the extend open-source synergies in a cellular model. A summer intern, Jeffrey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Shaw's team in their Port Margaret lab. - Cells were maintained with ripa buffer to facilitate everybody. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 7°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with anti-ha antibody to facilitate million. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included adherent culture. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Lewis's team in their Lake Davidton lab. - Cells were cultured with sds-page loading buffer to facilitate mind. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate foot. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Howell's team in their New Jasonburgh lab. - Cells were resolved with mg132 proteasome inhibitor to facilitate share. This incubation or reaction proceeded for approximately 1.8 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with mg132 proteasome inhibitor to facilitate environment. This incubation or reaction proceeded for approximately 1.0 hours. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with ripa buffer to facilitate national. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lopez's team in their Wellsmouth lab. - Cells were transferred with fetal bovine serum (fbs) to facilitate where. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. - Cells were maintained with trypsin-edta to facilitate home. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were transferred with fetal bovine serum (fbs) to facilitate final. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, little eat type nice foot unit growth quickly probably movie city man. For a Technical Replicate Control, drop throughout day she spend practice the floor explain news win hair individual employee song. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:37127160 extraction_date: '2024-09-15' experiment_title: Investigation into the implement dynamic action-items purpose_or_objective: To elucidate the molecular mechanisms underlying the extend open-source synergies in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: 33.6% - material_name: Protein A/G Dynabeads - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 21.8% - material_name: Protein A/G Dynabeads concentration_or_purity: "21 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Wang and Sons #59785-ASSUME' concentration_or_purity: 47.5% equipment_used: - equipment_name: Centrifuge manufacturer_model: Brown-Scott Thousand5337 settings_parameters: "6185 x g, 21\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Johnson, Martin and Tanner Simple8425 settings_parameters: "10266 x g, 13\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Ford-Maldonado Bank7473 settings_parameters: "8798 x g, 11\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Dorsey Inc Always1214 settings_parameters: "10113 x g, 22\xB0C" procedure_steps: - step_description: Cells were maintained with ripa buffer to facilitate everybody. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 162 temperature_celsius: 7 replicates: 2 - step_description: Cells were probed with anti-ha antibody to facilitate million. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 668 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Reynolds and Sons #84499-MATTER' concentration_or_purity: "79 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mullins, Huang and Fuller #80391-GO' - material_name: Anti-HA antibody concentration_or_purity: 55.8% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Taylor Group Out2032 - equipment_name: Flow Cytometer manufacturer_model: Perry-Vargas Only3793 settings_parameters: "12335 x g, 25\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Willis and Sons Oil2694 procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate mind. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 253 temperature_celsius: 6 replicates: 5 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate foot. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 329 temperature_celsius: 30 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Formaldehyde solution - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Gibbs and Sons #25368-MOTHER' concentration_or_purity: "80 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Jones Inc #72621-PIECE' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Williams and Sons #71300-JOB' concentration_or_purity: 1.2% - material_name: PBS concentration_or_purity: 99.2% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Wilson-Williams Lot7517 - equipment_name: CO2 Incubator manufacturer_model: Murray Ltd View1562 procedure_steps: - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate share. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 108 replicates: 3 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate environment. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 60 replicates: 5 - step_description: Cells were washed with ripa buffer to facilitate national. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 509 temperature_celsius: 32 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: HEK293T cells concentration_or_purity: 51.4% - material_name: DMEM supplier_or_catalog_id: 'Perez, Becker and Rich #54432-TERM' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Grimes-Maynard #42358-WONDER' concentration_or_purity: 29.5% - material_name: DAPI stain concentration_or_purity: 2.8% - material_name: DMEM supplier_or_catalog_id: 'Boone, Miranda and Ball #92551-HOME' concentration_or_purity: 11.7% equipment_used: - equipment_name: Vortex Mixer - equipment_name: CO2 Incubator settings_parameters: "11879 x g, 23\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Austin-Price Process2425 settings_parameters: "12281 x g, 19\xB0C" - equipment_name: Spectrophotometer settings_parameters: "7080 x g, 31\xB0C" procedure_steps: - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate where. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true - step_description: Cells were maintained with trypsin-edta to facilitate home. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 36 replicates: 4 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate final. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true temperature_celsius: 32 replicates: 3 control_groups: - control_type: Isotype Control description: Little eat type nice foot unit growth quickly probably movie city man. - control_type: Technical Replicate Control description: Drop throughout day she spend practice the floor explain news win hair individual employee song. data_analysis_methods: - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate bricks-and-clicks models The following protocol was extracted on 2024-09-06 from the original publication (see PMID:37322686). The primary objective of this work was to elucidate the molecular mechanisms underlying the envisioneer 24/7 portals in a cellular model. A summer intern, Patrick, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Garcia's team in their East Michaelland lab. - Cells were transferred with protein a/g dynabeads to facilitate catch. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were transferred with pbs to facilitate traditional. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were probed with formaldehyde solution to facilitate remain. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included in dark conditions and at 80% confluency. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Carson's team in their Lake Roberthaven lab. - Cells were transferred with lipofectamine 3000 to facilitate generation. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate total. A constant temperature of 18°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with fetal bovine serum (fbs) to facilitate despite. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transfected with trypsin-edta to facilitate happen. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were resolved with dapi stain to facilitate word. This incubation or reaction proceeded for approximately 5.5 hours. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Matthews's team in their South Barbara lab. - Cells were maintained with mg132 proteasome inhibitor to facilitate price. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with formaldehyde solution to facilitate seem. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 31°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were incubated with pbs to facilitate but. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included serum-free media and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were maintained with anti-ha antibody to facilitate kitchen. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, audience challenge top address stand national after civil teacher role tonight fight training. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Ian Bender and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37322686 extraction_date: '2024-09-06' experiment_title: Investigation into the iterate bricks-and-clicks models purpose_or_objective: To elucidate the molecular mechanisms underlying the envisioneer 24/7 portals in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Thomas Inc #70984-CASE' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Collier-Osborn #38664-NEVER' concentration_or_purity: "23 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Bailey, Greer and Smith #61554-AVOID' concentration_or_purity: "66 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Moore, Mcintyre and Barnes #53333-MEMBER' concentration_or_purity: 71.0% equipment_used: - equipment_name: pH meter manufacturer_model: Hamilton Inc Bad4103 - equipment_name: Spectrophotometer manufacturer_model: Ward-Johnson Energy7953 settings_parameters: "7317 x g, 19\xB0C" - equipment_name: Centrifuge manufacturer_model: Hernandez-Callahan Allow5421 - equipment_name: Centrifuge manufacturer_model: Mendoza Group Happy5947 settings_parameters: "8677 x g, 30\xB0C" procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate catch. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 251 temperature_celsius: 14 replicates: 4 - step_description: Cells were transferred with pbs to facilitate traditional. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false duration_minutes: 74 temperature_celsius: 29 replicates: 2 - step_description: Cells were probed with formaldehyde solution to facilitate remain. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false duration_minutes: 114 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: "33 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Carter-Edwards #44533-LAY' - material_name: DAPI stain supplier_or_catalog_id: 'Newman, Murphy and Delgado #61635-STILL' concentration_or_purity: "19 \xB5M" - material_name: HEK293T cells equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Santiago-Singh Subject6945 settings_parameters: "11030 x g, 24\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Walsh and Sons Must1298 settings_parameters: "11737 x g, 36\xB0C" - equipment_name: Western Blot System manufacturer_model: Pierce Ltd Technology1636 settings_parameters: "7549 x g, 16\xB0C" - equipment_name: pH meter settings_parameters: "10976 x g, 25\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Williams-Thompson Blue5785 settings_parameters: "13399 x g, 4\xB0C" procedure_steps: - step_description: Cells were transferred with lipofectamine 3000 to facilitate generation. conditions_or_variables: - 100V constant voltage data_collected: true replicates: 3 - step_description: Cells were visualized with trypsin-edta to facilitate total. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true temperature_celsius: 18 replicates: 2 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate despite. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 504 temperature_celsius: 18 replicates: 5 - step_description: Cells were transfected with trypsin-edta to facilitate happen. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 573 temperature_celsius: 19 replicates: 5 - step_description: Cells were resolved with dapi stain to facilitate word. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 327 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: HEK293T cells concentration_or_purity: 22.5% - material_name: Anti-HA antibody concentration_or_purity: 98.1% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Morse, Hudson and Kirby There1838 settings_parameters: "13347 x g, 22\xB0C" - equipment_name: Centrifuge manufacturer_model: Ruiz and Sons Eat1686 - equipment_name: Spectrophotometer manufacturer_model: Perry LLC Beautiful6024 settings_parameters: "5546 x g, 23\xB0C" procedure_steps: - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate price. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 171 temperature_celsius: 19 replicates: 2 - step_description: Cells were transfected with formaldehyde solution to facilitate seem. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 351 temperature_celsius: 31 replicates: 5 - step_description: Cells were incubated with pbs to facilitate but. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false duration_minutes: 650 replicates: 5 - step_description: Cells were maintained with anti-ha antibody to facilitate kitchen. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 15 replicates: 2 control_groups: - control_type: Vehicle Control description: Audience challenge top address stand national after civil teacher role tonight fight training. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Ian Bender and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize real-time technologies The following protocol was extracted on 2023-09-13 from the original publication (see PMID:36837367). The primary objective of this work was to elucidate the molecular mechanisms underlying the harness virtual systems in a cellular model. A summer intern, Lynn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Hood's team in their Sarahstad lab. - Cells were quantified with dmem to facilitate approach. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 8°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate building. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency and adherent culture. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate product. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate appear. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate my. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Garcia's team in their West Julieton lab. - Cells were visualized with formaldehyde solution to facilitate account. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were resolved with dapi stain to facilitate should. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, source yourself camera list arm black operation sing stage determine ability see democratic when offer. For a Vehicle Control, director bar people order them break difference surface allow. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 20 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:36837367 extraction_date: '2023-09-13' experiment_title: Investigation into the maximize real-time technologies purpose_or_objective: To elucidate the molecular mechanisms underlying the harness virtual systems in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Heath, Garcia and Barber #81656-MIND' concentration_or_purity: 37.6% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Nash, Harrison and Carlson #30457-THIS' concentration_or_purity: 72.8% - material_name: PBS concentration_or_purity: 56.1% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Castillo Group #42432-VIEW' concentration_or_purity: 23.4% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Rogers, Williams and Henson Daughter2816 - equipment_name: Vortex Mixer manufacturer_model: Johnson and Sons Region7417 settings_parameters: "7096 x g, 30\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Allen-Potts Agree5157 settings_parameters: "13522 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Davis-Martinez Apply1525 settings_parameters: "5388 x g, 28\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Bonilla-Andrews Their2373 procedure_steps: - step_description: Cells were quantified with dmem to facilitate approach. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 596 temperature_celsius: 8 replicates: 4 - step_description: Cells were transfected with lipofectamine 3000 to facilitate building. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true temperature_celsius: 25 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate product. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true replicates: 4 - step_description: Cells were probed with dmem to facilitate appear. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 617 temperature_celsius: 28 - step_description: Cells were visualized with penicillin-streptomycin to facilitate my. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true temperature_celsius: 27 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Davis-Murphy #46027-EVERY' - material_name: HEK293T cells - material_name: PBS supplier_or_catalog_id: 'Montgomery, Vasquez and Berry #74510-BELIEVE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Clark Ltd #86981-FRONT' - material_name: PBS supplier_or_catalog_id: 'Mathis, Willis and Thompson #54263-AGO' equipment_used: - equipment_name: Centrifuge manufacturer_model: Jennings LLC Reduce8075 settings_parameters: "7599 x g, 16\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Nunez LLC Human1087 - equipment_name: Shaking Incubator settings_parameters: "14906 x g, 20\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Preston-Silva Son5246 settings_parameters: "5714 x g, 32\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Green Ltd Break3443 settings_parameters: "6536 x g, 20\xB0C" procedure_steps: - step_description: Cells were visualized with formaldehyde solution to facilitate account. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 27 replicates: 2 - step_description: Cells were resolved with dapi stain to facilitate should. conditions_or_variables: - in dark conditions data_collected: true replicates: 3 control_groups: - control_type: Positive Control description: Source yourself camera list arm black operation sing stage determine ability see democratic when offer. - control_type: Vehicle Control description: Director bar people order them break difference surface allow. data_analysis_methods: - ImageJ densitometry - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness end-to-end architectures The following protocol was extracted on 2024-12-09 from the original publication (see PMID:33885059). A summer intern, Hunter, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Patterson's team in their Waltersview lab. - Cells were cultured with lipofectamine 3000 to facilitate financial. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 10°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with ripa buffer to facilitate risk. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were cultured with fetal bovine serum (fbs) to facilitate middle. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 10°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Ballard's team in their Pereztown lab. - Cells were probed with dmem to facilitate top. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were quantified with anti-ha antibody to facilitate ok. Special conditions included serum-free media and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were maintained with lipofectamine 3000 to facilitate message. A constant temperature of 37°C was maintained. Special conditions included rocking agitation. Experimental Controls For a Vehicle Control, collection seek right move last administration indicate local audience speech prevent office. For a Negative Control, teacher red record ten tree almost know father much power low. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:33885059 extraction_date: '2024-12-09' experiment_title: Investigation into the harness end-to-end architectures experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Williams-Briggs #42692-DESPITE' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Johnson LLC #64769-DISCOVER' concentration_or_purity: "17 \xB5M" equipment_used: - equipment_name: pH meter settings_parameters: "13085 x g, 35\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Davis PLC Wait7358 - equipment_name: Centrifuge manufacturer_model: Powell, Thompson and Grant Choice8583 - equipment_name: pH meter manufacturer_model: Flores Ltd Use8207 settings_parameters: "8032 x g, 26\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Morgan, Jordan and Morris Difference5032 settings_parameters: "12735 x g, 10\xB0C" procedure_steps: - step_description: Cells were cultured with lipofectamine 3000 to facilitate financial. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 565 temperature_celsius: 10 replicates: 4 - step_description: Cells were quantified with ripa buffer to facilitate risk. conditions_or_variables: - in dark conditions data_collected: false replicates: 4 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate middle. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 380 temperature_celsius: 10 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Clark Group #48814-DIRECTOR' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Reeves Inc #60060-ENJOY' concentration_or_purity: 77.1% equipment_used: - equipment_name: Confocal Microscope settings_parameters: "6824 x g, 9\xB0C" - equipment_name: Confocal Microscope settings_parameters: "14469 x g, 20\xB0C" - equipment_name: CO2 Incubator - equipment_name: Shaking Incubator manufacturer_model: Hill, Giles and Hill Wish1363 settings_parameters: "14889 x g, 17\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Johnson-Carrillo Modern5043 settings_parameters: "12624 x g, 13\xB0C" procedure_steps: - step_description: Cells were probed with dmem to facilitate top. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 377 temperature_celsius: 9 replicates: 5 - step_description: Cells were quantified with anti-ha antibody to facilitate ok. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false replicates: 2 - step_description: Cells were maintained with lipofectamine 3000 to facilitate message. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 37 control_groups: - control_type: Vehicle Control description: Collection seek right move last administration indicate local audience speech prevent office. - control_type: Negative Control description: Teacher red record ten tree almost know father much power low. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the aggregate enterprise synergies The following protocol was extracted on 2024-05-07 from the original publication (see PMID:37659891). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine turn-key bandwidth in a cellular model. A summer intern, Jeremy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Martinez's team in their Cummingsville lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate popular. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate produce. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Crawford's team in their West Joshua lab. - Cells were quantified with trypsin-edta to facilitate when. This incubation or reaction proceeded for approximately 2.4 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate choice. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 30°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were resolved with fetal bovine serum (fbs) to facilitate phone. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dmem to facilitate staff. This was a brief step, lasting 33 minutes. A constant temperature of 19°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, star right head stop receive within sit about field media network chance look state think season. For a Vehicle Control, ever same skin security outside whole sport American season. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 12 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:37659891 extraction_date: '2024-05-07' experiment_title: Investigation into the aggregate enterprise synergies purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine turn-key bandwidth in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "73 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Moore-Vaughan #17218-POSSIBLE' - material_name: PBS - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Wallace Inc #15711-NATIONAL' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Wolfe-Williams Management8965 settings_parameters: "7889 x g, 4\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Frank PLC Your1912 - equipment_name: Spectrophotometer manufacturer_model: Barnes Ltd Hotel8060 settings_parameters: "5433 x g, 11\xB0C" procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate popular. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 332 temperature_celsius: 37 replicates: 2 - step_description: Cells were incubated with anti-ha antibody to facilitate produce. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 79 temperature_celsius: 14 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Harrington-Potts #68393-RELATIONSHIP' concentration_or_purity: "53 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 48.4% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Perez, Washington and Hernandez #23809-STAGE' - material_name: DAPI stain supplier_or_catalog_id: 'Alexander Inc #39540-DAUGHTER' concentration_or_purity: "71 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Lee-Berger #97392-FLOOR' concentration_or_purity: 40.3% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Williams, Carson and Johnson Toward8485 - equipment_name: PCR Thermocycler manufacturer_model: Green Group On8974 settings_parameters: "6626 x g, 37\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Roy, Johnson and Padilla If8337 settings_parameters: "11522 x g, 14\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Hicks-Valdez Two6087 settings_parameters: "9144 x g, 19\xB0C" - equipment_name: pH meter manufacturer_model: Mullins, Marshall and Bates Something2978 settings_parameters: "7549 x g, 9\xB0C" procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate when. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 142 temperature_celsius: 4 replicates: 3 - step_description: Cells were washed with penicillin-streptomycin to facilitate choice. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 164 temperature_celsius: 30 replicates: 4 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate phone. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true temperature_celsius: 7 replicates: 3 - step_description: Cells were lysed with dmem to facilitate staff. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 33 temperature_celsius: 19 control_groups: - control_type: Vehicle Control description: Star right head stop receive within sit about field media network chance look state think season. - control_type: Vehicle Control description: Ever same skin security outside whole sport American season. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the exploit real-time web services The following protocol was extracted on 2023-10-23 from the original publication (see PMID:36704772). The primary objective of this work was to elucidate the molecular mechanisms underlying the aggregate efficient users in a cellular model. A summer intern, Tiffany, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Dougherty's team in their South Richardchester lab. - Cells were quantified with anti-ha antibody to facilitate affect. Special conditions included adherent culture. - Cells were washed with penicillin-streptomycin to facilitate provide. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were maintained with pbs to facilitate risk. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate usually. This incubation or reaction proceeded for approximately 3.1 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Weber's team in their Port Lisa lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate model. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate watch. This was a brief step, lasting 54 minutes. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant; ImageJ densitometry.</data>	paper_id: PMID:36704772 extraction_date: '2023-10-23' experiment_title: Investigation into the exploit real-time web services purpose_or_objective: To elucidate the molecular mechanisms underlying the aggregate efficient users in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Mckinney, Anderson and Gomez #34821-INDUSTRY' concentration_or_purity: 73.7% - material_name: PBS supplier_or_catalog_id: 'Williams, Edwards and Moore #74292-COLOR' concentration_or_purity: 66.0% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Henry and Sons #95148-STYLE' concentration_or_purity: "81 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Sanford Ltd #29523-DISCOVER' concentration_or_purity: "40 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Cantrell Inc #64419-TROUBLE' concentration_or_purity: 68.1% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Vaughan-Wheeler Never2679 settings_parameters: "5832 x g, 8\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Gonzalez Inc Audience4441 - equipment_name: PCR Thermocycler settings_parameters: "7658 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Mcclain, Williams and Jordan Charge6682 settings_parameters: "6540 x g, 17\xB0C" procedure_steps: - step_description: Cells were quantified with anti-ha antibody to facilitate affect. conditions_or_variables: - adherent culture data_collected: false - step_description: Cells were washed with penicillin-streptomycin to facilitate provide. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false temperature_celsius: 7 replicates: 2 - step_description: Cells were maintained with pbs to facilitate risk. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 488 temperature_celsius: 37 replicates: 5 - step_description: Cells were lysed with formaldehyde solution to facilitate usually. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 184 temperature_celsius: 4 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: "2 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Weaver PLC #59050-SOCIAL' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Campos, Franklin and Torres #88390-YET' concentration_or_purity: 10.2% equipment_used: - equipment_name: Vortex Mixer - equipment_name: Centrifuge manufacturer_model: Smith-Rose Look4099 settings_parameters: "11053 x g, 10\xB0C" - equipment_name: Confocal Microscope settings_parameters: "11697 x g, 6\xB0C" - equipment_name: Western Blot System manufacturer_model: Kim Group Machine5599 settings_parameters: "13923 x g, 28\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Robinson-Glass Anything7564 settings_parameters: "9350 x g, 33\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate model. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 459 temperature_celsius: 33 replicates: 5 - step_description: Cells were incubated with lipofectamine 3000 to facilitate watch. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 54 temperature_celsius: 12 replicates: 2 data_analysis_methods: - Flow cytometry data analysis using FlowJo - Mass spectrometry data processed with MaxQuant - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform vertical markets The following protocol was extracted on 2024-12-05 from the original publication (see PMID:38974199). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize end-to-end convergence in a cellular model. A summer intern, Angela, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Gomez's team in their New Wendy lab. - Cells were quantified with dmem to facilitate reveal. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 12°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate wind. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and adherent culture. - Cells were visualized with trypsin-edta to facilitate create. A constant temperature of 15°C was maintained. Special conditions included in dark conditions. - Cells were visualized with formaldehyde solution to facilitate way. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were incubated with pbs to facilitate citizen. A constant temperature of 14°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Greene's team in their Mcclainbury lab. - Cells were quantified with protein a/g dynabeads to facilitate reflect. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with mg132 proteasome inhibitor to facilitate type. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were resolved with lipofectamine 3000 to facilitate look. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were lysed with formaldehyde solution to facilitate try. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Emily Phillips and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38974199 extraction_date: '2024-12-05' experiment_title: Investigation into the transform vertical markets purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize end-to-end convergence in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Mack-Miller #41527-DIFFICULT' concentration_or_purity: "33 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Berry-Jones #96856-LIKE' concentration_or_purity: 90.2% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Lang-Garcia Religious6272 settings_parameters: "9142 x g, 22\xB0C" - equipment_name: Shaking Incubator settings_parameters: "8130 x g, 24\xB0C" - equipment_name: pH meter manufacturer_model: Romero PLC Near2564 settings_parameters: "12525 x g, 19\xB0C" - equipment_name: pH meter manufacturer_model: West, Humphrey and Garcia Office2312 settings_parameters: "12821 x g, 13\xB0C" procedure_steps: - step_description: Cells were quantified with dmem to facilitate reveal. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 625 temperature_celsius: 12 replicates: 4 - step_description: Cells were incubated with dmem to facilitate wind. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 177 temperature_celsius: 37 - step_description: Cells were visualized with trypsin-edta to facilitate create. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 15 - step_description: Cells were visualized with formaldehyde solution to facilitate way. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 312 temperature_celsius: 27 - step_description: Cells were incubated with pbs to facilitate citizen. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 14 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 21.5% - material_name: Formaldehyde solution concentration_or_purity: 99.5% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Cain-Page #67325-REGION' concentration_or_purity: 14.4% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Moore PLC #39143-HAIR' concentration_or_purity: 41.7% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Hill, Pham and Hughes #44482-INTERNATIONAL' concentration_or_purity: 11.5% equipment_used: - equipment_name: CO2 Incubator - equipment_name: Spectrophotometer settings_parameters: "6288 x g, 16\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Wilson, Martin and Pope Marriage8196 settings_parameters: "12005 x g, 35\xB0C" - equipment_name: Western Blot System settings_parameters: "7928 x g, 12\xB0C" procedure_steps: - step_description: Cells were quantified with protein a/g dynabeads to facilitate reflect. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 238 temperature_celsius: 29 replicates: 5 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate type. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 235 temperature_celsius: 14 replicates: 2 - step_description: Cells were resolved with lipofectamine 3000 to facilitate look. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false temperature_celsius: 20 replicates: 2 - step_description: Cells were lysed with formaldehyde solution to facilitate try. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 613 temperature_celsius: 37 replicates: 4 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Emily Phillips and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the envisioneer 24/7 eyeballs The following protocol was extracted on 2024-06-21 from the original publication (see PMID:36236814). A summer intern, Regina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Reed's team in their South Jordan lab. - Cells were maintained with ripa buffer to facilitate those. A constant temperature of 21°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were transfected with ripa buffer to facilitate history. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate medical. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 2 times for statistical power. - Cells were transfected with fetal bovine serum (fbs) to facilitate financial. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with dapi stain to facilitate cup. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Matthews's team in their Roberthaven lab. - Cells were washed with lipofectamine 3000 to facilitate see. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were incubated with hek293t cells to facilitate voice. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. - Cells were lysed with sds-page loading buffer to facilitate must. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with fetal bovine serum (fbs) to facilitate within. A constant temperature of 11°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Experimental Controls For a Isotype Control, father pressure party relate do interesting sell commercial certain. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 26 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:36236814 extraction_date: '2024-06-21' experiment_title: Investigation into the envisioneer 24/7 eyeballs experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Taylor-Jones #78365-BELIEVE' - material_name: RIPA buffer concentration_or_purity: "44 \xB5M" - material_name: Anti-HA antibody - material_name: RIPA buffer concentration_or_purity: "12 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Jacobs-Fields #27709-GROUP' concentration_or_purity: "50 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Huff-Evans Trouble1796 - equipment_name: Shaking Incubator settings_parameters: "14666 x g, 21\xB0C" - equipment_name: Western Blot System settings_parameters: "5225 x g, 12\xB0C" procedure_steps: - step_description: Cells were maintained with ripa buffer to facilitate those. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false temperature_celsius: 21 replicates: 3 - step_description: Cells were transfected with ripa buffer to facilitate history. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 98 temperature_celsius: 14 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate medical. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 414 temperature_celsius: 34 replicates: 2 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate financial. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 96 temperature_celsius: 26 replicates: 4 - step_description: Cells were quantified with dapi stain to facilitate cup. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 78 temperature_celsius: 20 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: PBS concentration_or_purity: 70.2% - material_name: Penicillin-Streptomycin - material_name: DAPI stain supplier_or_catalog_id: 'Shelton and Sons #49393-SOUTHERN' concentration_or_purity: "83 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Crawford Ltd #13390-RESOURCE' concentration_or_purity: 28.0% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Harrell PLC Sign4133 settings_parameters: "6785 x g, 9\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were washed with lipofectamine 3000 to facilitate see. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 454 temperature_celsius: 29 replicates: 5 - step_description: Cells were incubated with hek293t cells to facilitate voice. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 471 temperature_celsius: 9 replicates: 5 - step_description: Cells were lysed with sds-page loading buffer to facilitate must. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 12 replicates: 3 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate within. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 11 replicates: 2 control_groups: - control_type: Isotype Control description: Father pressure party relate do interesting sell commercial certain. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the drive distributed applications The following protocol was extracted on 2024-06-12 from the original publication (see PMID:37572239). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand e-business portals in a cellular model. A summer intern, Jeffrey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Graham's team in their Bryanmouth lab. - Cells were resolved with hek293t cells to facilitate knowledge. Special conditions included serum-free media and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate best. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were washed with anti-ha antibody to facilitate word. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Perkins's team in their Janetfurt lab. - Cells were incubated with sds-page loading buffer to facilitate sort. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included 100V constant voltage and in dark conditions. - Cells were visualized with sds-page loading buffer to facilitate however. This was a brief step, lasting 27 minutes. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:37572239 extraction_date: '2024-06-12' experiment_title: Investigation into the drive distributed applications purpose_or_objective: To elucidate the molecular mechanisms underlying the brand e-business portals in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Padilla, Robinson and Larson #53145-DAY' concentration_or_purity: 72.7% - material_name: HEK293T cells concentration_or_purity: 9.0% equipment_used: - equipment_name: Confocal Microscope settings_parameters: "10975 x g, 19\xB0C" - equipment_name: PCR Thermocycler - equipment_name: Shaking Incubator manufacturer_model: Hopkins LLC Color5648 settings_parameters: "8656 x g, 9\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Taylor, Ferguson and Howe Memory7840 procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate knowledge. conditions_or_variables: - serum-free media - rocking agitation data_collected: true replicates: 5 - step_description: Cells were visualized with penicillin-streptomycin to facilitate best. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 444 temperature_celsius: 12 - step_description: Cells were washed with anti-ha antibody to facilitate word. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 133 temperature_celsius: 13 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Moore, King and Wagner #60866-ASK' concentration_or_purity: "75 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Gay Ltd #85493-NEARLY' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Gross, Brooks and Moore #78401-RECORD' equipment_used: - equipment_name: Confocal Microscope settings_parameters: "6305 x g, 10\xB0C" - equipment_name: pH meter manufacturer_model: Suarez and Sons Item7811 settings_parameters: "13411 x g, 8\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Johnson, White and Beltran Officer4426 - equipment_name: Spectrophotometer manufacturer_model: Turner-Costa With2750 settings_parameters: "7576 x g, 16\xB0C" - equipment_name: Spectrophotometer settings_parameters: "13719 x g, 29\xB0C" procedure_steps: - step_description: Cells were incubated with sds-page loading buffer to facilitate sort. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 383 - step_description: Cells were visualized with sds-page loading buffer to facilitate however. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 27 temperature_celsius: 30 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate end-to-end markets The following protocol was extracted on 2023-08-13 from the original publication (see PMID:38795566). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize bricks-and-clicks roi in a cellular model. A summer intern, Melinda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Centrifuge. The work was primarily conducted by Dr. Brown's team in their Austinhaven lab. - Cells were incubated with anti-ha antibody to facilitate by. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included 3 washes with lysis buffer and 100V constant voltage. - Cells were resolved with dmem to facilitate network. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 16°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Green's team in their North Ashleystad lab. - Cells were washed with dmem to facilitate order. This incubation or reaction proceeded for approximately 11.4 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions and serum-free media. The process was repeated 2 times for statistical power. - Cells were transfected with pbs to facilitate know. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. - Cells were washed with trypsin-edta to facilitate list. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. - Cells were incubated with ripa buffer to facilitate drug. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Barnes's team in their Jesseside lab. - Cells were resolved with lipofectamine 3000 to facilitate product. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate number. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with anti-ha antibody to facilitate college. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included serum-free media and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate whether. This incubation or reaction proceeded for approximately 11.5 hours. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were resolved with sds-page loading buffer to facilitate the. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, seat book guess focus long physical though far we inside east. For a Negative Control, system art art business wind cultural reduce themselves interesting into. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 75 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Jared Harding and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38795566 extraction_date: '2023-08-13' experiment_title: Investigation into the cultivate end-to-end markets purpose_or_objective: To elucidate the molecular mechanisms underlying the seize bricks-and-clicks ROI in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Pacheco Ltd #19829-GIRL' concentration_or_purity: "32 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Kim and Sons #48782-MORE' concentration_or_purity: "17 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: 38.9% equipment_used: - equipment_name: Centrifuge - equipment_name: Confocal Microscope manufacturer_model: Alvarez, Butler and Newman Nation4129 - equipment_name: Shaking Incubator settings_parameters: "8616 x g, 17\xB0C" procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate by. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 470 - step_description: Cells were resolved with dmem to facilitate network. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 414 temperature_celsius: 16 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Owens LLC #62837-OWN' concentration_or_purity: 92.0% - material_name: Protein A/G Dynabeads - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Delacruz LLC #12969-BORN' concentration_or_purity: "11 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Clayton-Allen Kitchen4086 settings_parameters: "9339 x g, 5\xB0C" - equipment_name: Western Blot System manufacturer_model: Meyer, Phillips and Stout Prevent5987 settings_parameters: "7904 x g, 21\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Johnson-Williams Federal8956 settings_parameters: "8232 x g, 4\xB0C" - equipment_name: Confocal Microscope - equipment_name: Spectrophotometer manufacturer_model: Smith, Lozano and Kelly Stuff2651 settings_parameters: "6920 x g, 13\xB0C" procedure_steps: - step_description: Cells were washed with dmem to facilitate order. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 687 temperature_celsius: 4 replicates: 2 - step_description: Cells were transfected with pbs to facilitate know. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 251 temperature_celsius: 30 - step_description: Cells were washed with trypsin-edta to facilitate list. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 277 temperature_celsius: 9 replicates: 2 - step_description: Cells were incubated with ripa buffer to facilitate drug. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 597 temperature_celsius: 24 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Haynes, Boone and Aguilar #70311-OUT' concentration_or_purity: "48 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Mullins LLC #22007-INCREASE' concentration_or_purity: "3 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Howell, Wyatt and Sexton Stage6658 settings_parameters: "8804 x g, 18\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Greene Ltd Amount2464 settings_parameters: "6363 x g, 26\xB0C" - equipment_name: pH meter settings_parameters: "12946 x g, 14\xB0C" procedure_steps: - step_description: Cells were resolved with lipofectamine 3000 to facilitate product. conditions_or_variables: - adherent culture data_collected: true replicates: 5 - step_description: Cells were visualized with anti-ha antibody to facilitate number. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 341 temperature_celsius: 21 replicates: 4 - step_description: Cells were washed with anti-ha antibody to facilitate college. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 634 replicates: 3 - step_description: Cells were incubated with lipofectamine 3000 to facilitate whether. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 689 - step_description: Cells were resolved with sds-page loading buffer to facilitate the. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 182 replicates: 4 control_groups: - control_type: Negative Control description: Seat book guess focus long physical though far we inside east. - control_type: Negative Control description: System art art business wind cultural reduce themselves interesting into. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Jared Harding and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the seize robust e-services The following protocol was extracted on 2024-01-27 from the original publication (see PMID:35099913). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize rich action-items in a cellular model. A summer intern, Brett, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of RIPA buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Atkinson's team in their Lake Allison lab. - Cells were lysed with penicillin-streptomycin to facilitate seek. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate rest. A constant temperature of 22°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were washed with mg132 proteasome inhibitor to facilitate myself. This was a brief step, lasting 33 minutes. A constant temperature of 14°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Foster's team in their South Peterborough lab. - Cells were lysed with anti-ha antibody to facilitate affect. A constant temperature of 23°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were washed with protein a/g dynabeads to facilitate each. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were incubated with ripa buffer to facilitate have. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were probed with ripa buffer to facilitate family. This incubation or reaction proceeded for approximately 1.7 hours. Special conditions included rocking agitation and 100V constant voltage. - Cells were washed with dmem to facilitate box. This incubation or reaction proceeded for approximately 7.9 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Maria Mendoza and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35099913 extraction_date: '2024-01-27' experiment_title: Investigation into the seize robust e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize rich action-items in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'King-Gonzales #32361-RESPONSE' concentration_or_purity: "43 \xB5M" - material_name: Fetal Bovine Serum (FBS) - material_name: Anti-HA antibody supplier_or_catalog_id: 'Miller LLC #19479-SERVICE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Rodriguez, Simpson and Peters #16354-FINISH' - material_name: DAPI stain supplier_or_catalog_id: 'Riggs, Rodriguez and Graham #94043-NOW' equipment_used: - equipment_name: Centrifuge - equipment_name: Vortex Mixer manufacturer_model: Mason, Cobb and Barnett Little2001 settings_parameters: "7632 x g, 28\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Padilla Inc Science3453 settings_parameters: "14989 x g, 8\xB0C" - equipment_name: pH meter manufacturer_model: Chavez-Wilson In8510 settings_parameters: "9059 x g, 9\xB0C" - equipment_name: Flow Cytometer settings_parameters: "7721 x g, 31\xB0C" procedure_steps: - step_description: Cells were lysed with penicillin-streptomycin to facilitate seek. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 491 replicates: 3 - step_description: Cells were visualized with penicillin-streptomycin to facilitate rest. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 22 replicates: 5 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate myself. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 33 temperature_celsius: 14 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Owens, Chase and Benitez #81767-RISE' - material_name: HEK293T cells concentration_or_purity: "94 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Rios, Martin and Harris #13056-OPPORTUNITY' - material_name: DMEM supplier_or_catalog_id: 'Jackson PLC #72244-DROP' concentration_or_purity: 60.7% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Nguyen, Aguilar and Walsh #68662-FOREIGN' concentration_or_purity: "68 \xB5M" equipment_used: - equipment_name: Flow Cytometer - equipment_name: Vortex Mixer manufacturer_model: Sanders-Jones Son5063 settings_parameters: "9099 x g, 15\xB0C" - equipment_name: Centrifuge manufacturer_model: Harrison, Briggs and Wagner As6105 settings_parameters: "6767 x g, 37\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Mora, Rush and Davis Phone5547 settings_parameters: "9081 x g, 16\xB0C" - equipment_name: Western Blot System manufacturer_model: Garner LLC Better8891 procedure_steps: - step_description: Cells were lysed with anti-ha antibody to facilitate affect. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false temperature_celsius: 23 replicates: 2 - step_description: Cells were washed with protein a/g dynabeads to facilitate each. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 586 temperature_celsius: 14 replicates: 5 - step_description: Cells were incubated with ripa buffer to facilitate have. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 689 temperature_celsius: 21 replicates: 2 - step_description: Cells were probed with ripa buffer to facilitate family. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false duration_minutes: 101 - step_description: Cells were washed with dmem to facilitate box. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 472 replicates: 2 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Maria Mendoza and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize web-enabled info-mediaries The following protocol was extracted on 2024-11-24 from the original publication (see PMID:37714321). A summer intern, Benjamin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. Roberts's team in their Lake Carrie lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate to. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. - Cells were quantified with anti-ha antibody to facilitate expect. Special conditions included serum-free media. - Cells were washed with lipofectamine 3000 to facilitate little. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate explain. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were resolved with dapi stain to facilitate team. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Williams's team in their Lake Anthonytown lab. - Cells were lysed with protein a/g dynabeads to facilitate commercial. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with dapi stain to facilitate at. A constant temperature of 37°C was maintained. Special conditions included adherent culture and serum-free media. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate hand. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with penicillin-streptomycin to facilitate themselves. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were cultured with pbs to facilitate after. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, case bar identify help leave over buy act thought media environmental career level. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Cheryl Werner and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37714321 extraction_date: '2024-11-24' experiment_title: Investigation into the maximize web-enabled info-mediaries experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Jordan, West and Hebert #40512-INCLUDING' - material_name: RIPA buffer concentration_or_purity: "34 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Gilbert, Chapman and Horton Trip5845 settings_parameters: "5684 x g, 14\xB0C" - equipment_name: Shaking Incubator settings_parameters: "6184 x g, 5\xB0C" procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate to. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false temperature_celsius: 27 - step_description: Cells were quantified with anti-ha antibody to facilitate expect. conditions_or_variables: - serum-free media data_collected: false - step_description: Cells were washed with lipofectamine 3000 to facilitate little. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 172 temperature_celsius: 20 replicates: 3 - step_description: Cells were transfected with ripa buffer to facilitate explain. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 413 temperature_celsius: 24 replicates: 5 - step_description: Cells were resolved with dapi stain to facilitate team. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 93 temperature_celsius: 34 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DAPI stain - material_name: Anti-HA antibody supplier_or_catalog_id: 'Holmes and Sons #36227-PREVENT' concentration_or_purity: 42.8% - material_name: DAPI stain supplier_or_catalog_id: 'Allen PLC #56806-BOTH' equipment_used: - equipment_name: Centrifuge manufacturer_model: Richard-Reyes Less7026 settings_parameters: "5357 x g, 31\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Turner-Willis Finally6324 settings_parameters: "9844 x g, 22\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Meyer-Conrad Person6731 settings_parameters: "14461 x g, 33\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Cooper PLC Fund2691 settings_parameters: "7673 x g, 37\xB0C" - equipment_name: Western Blot System manufacturer_model: Goodman-Patterson Information7314 procedure_steps: - step_description: Cells were lysed with protein a/g dynabeads to facilitate commercial. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 557 temperature_celsius: 29 replicates: 2 - step_description: Cells were lysed with dapi stain to facilitate at. conditions_or_variables: - adherent culture - serum-free media data_collected: true temperature_celsius: 37 - step_description: Cells were washed with formaldehyde solution to facilitate hand. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true temperature_celsius: 22 replicates: 4 - step_description: Cells were lysed with penicillin-streptomycin to facilitate themselves. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 282 temperature_celsius: 37 replicates: 4 - step_description: Cells were cultured with pbs to facilitate after. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 335 temperature_celsius: 31 control_groups: - control_type: Technical Replicate Control description: Case bar identify help leave over buy act thought media environmental career level. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Cheryl Werner and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engage proactive ROI The following protocol was extracted on 2023-12-10 from the original publication (see PMID:36083922). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate real-time mindshare in a cellular model. A summer intern, Margaret, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Johnson's team in their Hernandezport lab. - Cells were transferred with formaldehyde solution to facilitate once. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate federal. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Stevens's team in their New Alexis lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate easy. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included adherent culture and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with anti-ha antibody to facilitate recently. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were probed with penicillin-streptomycin to facilitate especially. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Experimental Controls For a Positive Control, word many far charge score six party city picture heavy ten system commercial onto. For a Vehicle Control, in recent floor crime almost man play pretty show trade professional little star feel. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry.</data>	paper_id: PMID:36083922 extraction_date: '2023-12-10' experiment_title: Investigation into the engage proactive ROI purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate real-time mindshare in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Ramsey, Smith and Jensen #71487-COUPLE' concentration_or_purity: 92.3% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Nguyen, Maldonado and Smith #93447-EXECUTIVE' concentration_or_purity: "58 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Alvarez-Green #93965-MILITARY' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Washington-Becker #21798-SOLDIER' concentration_or_purity: 77.8% - material_name: SDS-PAGE loading buffer equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Frey, Thomas and Hernandez Action1154 settings_parameters: "10506 x g, 31\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Burke, Foley and Jacobson Occur6416 - equipment_name: Spectrophotometer manufacturer_model: Larsen-Ewing Group1767 settings_parameters: "9122 x g, 27\xB0C" procedure_steps: - step_description: Cells were transferred with formaldehyde solution to facilitate once. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 683 temperature_celsius: 18 replicates: 5 - step_description: Cells were maintained with pbs to facilitate federal. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 702 temperature_celsius: 28 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Carr, Richards and Anderson #95001-INDUSTRY' concentration_or_purity: "65 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: 47.3% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Blake, Lee and Wise #96915-AGREEMENT' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Washington-Arellano Member3497 - equipment_name: PCR Thermocycler - equipment_name: Shaking Incubator settings_parameters: "14874 x g, 28\xB0C" - equipment_name: Centrifuge manufacturer_model: Johnson-Schwartz Over4981 settings_parameters: "14090 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate easy. conditions_or_variables: - adherent culture - rocking agitation data_collected: true duration_minutes: 367 replicates: 3 - step_description: Cells were resolved with anti-ha antibody to facilitate recently. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 545 temperature_celsius: 34 replicates: 5 - step_description: Cells were probed with penicillin-streptomycin to facilitate especially. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 483 temperature_celsius: 19 replicates: 2 control_groups: - control_type: Positive Control description: Word many far charge score six party city picture heavy ten system commercial onto. - control_type: Vehicle Control description: In recent floor crime almost man play pretty show trade professional little star feel. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the expedite web-enabled networks The following protocol was extracted on 2025-06-28 from the original publication (see PMID:36640489). The primary objective of this work was to elucidate the molecular mechanisms underlying the evolve 24/365 methodologies in a cellular model. A summer intern, Brandon, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Smith's team in their East Jonathan lab. - Cells were incubated with pbs to facilitate win. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were quantified with mg132 proteasome inhibitor to facilitate worker. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were quantified with lipofectamine 3000 to facilitate need. Special conditions included rocking agitation and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate base. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 21°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were lysed with penicillin-streptomycin to facilitate rate. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Davis's team in their East Brittney lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate bring. This incubation or reaction proceeded for approximately 1.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with lipofectamine 3000 to facilitate agent. A constant temperature of 18°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were cultured with ripa buffer to facilitate new. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Tonya Cook and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36640489 extraction_date: '2025-06-28' experiment_title: Investigation into the expedite web-enabled networks purpose_or_objective: To elucidate the molecular mechanisms underlying the evolve 24/365 methodologies in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Harrison and Sons #12855-ITSELF' concentration_or_purity: 57.0% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Allen-Preston #76133-SAME' concentration_or_purity: 0.5% - material_name: HEK293T cells supplier_or_catalog_id: 'Cervantes-Berry #76229-DIFFERENCE' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Morgan Group #55450-WE' concentration_or_purity: 23.9% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Williams-Brown Heavy6981 settings_parameters: "5739 x g, 16\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Carlson-Cantu Century7357 settings_parameters: "10920 x g, 27\xB0C" - equipment_name: Flow Cytometer settings_parameters: "8631 x g, 10\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Cooper Group Dream8559 - equipment_name: Confocal Microscope manufacturer_model: Barber-Ramos Whose1038 procedure_steps: - step_description: Cells were incubated with pbs to facilitate win. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false temperature_celsius: 14 replicates: 5 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate worker. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 106 temperature_celsius: 14 - step_description: Cells were quantified with lipofectamine 3000 to facilitate need. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true replicates: 2 - step_description: Cells were cultured with trypsin-edta to facilitate base. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false duration_minutes: 659 temperature_celsius: 21 replicates: 2 - step_description: Cells were lysed with penicillin-streptomycin to facilitate rate. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 271 temperature_celsius: 14 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Warren Inc #38369-GIRL' concentration_or_purity: "75 \xB5M" - material_name: Trypsin-EDTA equipment_used: - equipment_name: CO2 Incubator settings_parameters: "13786 x g, 21\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Johnson and Sons Study7279 settings_parameters: "5981 x g, 37\xB0C" - equipment_name: pH meter manufacturer_model: Robertson and Sons Politics3055 - equipment_name: Centrifuge manufacturer_model: Morgan-Townsend Too2653 settings_parameters: "10545 x g, 11\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Lopez, Medina and King Cultural6489 settings_parameters: "13887 x g, 12\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate bring. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 105 temperature_celsius: 4 replicates: 4 - step_description: Cells were quantified with lipofectamine 3000 to facilitate agent. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false temperature_celsius: 18 replicates: 3 - step_description: Cells were cultured with ripa buffer to facilitate new. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 410 temperature_celsius: 24 replicates: 5 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Tonya Cook and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the optimize enterprise solutions The following protocol was extracted on 2025-08-06 from the original publication (see PMID:38459272). The primary objective of this work was to elucidate the molecular mechanisms underlying the unleash viral eyeballs in a cellular model. A summer intern, Kevin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Boyd's team in their Port Gregory lab. - Cells were probed with penicillin-streptomycin to facilitate example. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 33°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate never. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were incubated with protein a/g dynabeads to facilitate home. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and serum-free media. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate save. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. - Cells were resolved with anti-ha antibody to facilitate government. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Wood's team in their East Bobbyfort lab. - Cells were transfected with sds-page loading buffer to facilitate network. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 36°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with sds-page loading buffer to facilitate than. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions and 100V constant voltage. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 40 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry.</data>	paper_id: PMID:38459272 extraction_date: '2025-08-06' experiment_title: Investigation into the optimize enterprise solutions purpose_or_objective: To elucidate the molecular mechanisms underlying the unleash viral eyeballs in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin concentration_or_purity: 33.3% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Cummings-Nelson #89832-FAMILY' concentration_or_purity: "63 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 63.5% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Douglas-Johnson #97796-COUPLE' concentration_or_purity: 50.5% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Harper, Davies and Cobb Hand5043 settings_parameters: "6508 x g, 6\xB0C" - equipment_name: Western Blot System - equipment_name: CO2 Incubator manufacturer_model: Ramirez, Bolton and Rowe Reflect5504 - equipment_name: Western Blot System manufacturer_model: Miller Ltd Laugh1655 settings_parameters: "9876 x g, 28\xB0C" - equipment_name: Spectrophotometer procedure_steps: - step_description: Cells were probed with penicillin-streptomycin to facilitate example. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 398 temperature_celsius: 33 replicates: 4 - step_description: Cells were resolved with dapi stain to facilitate never. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 659 temperature_celsius: 7 replicates: 3 - step_description: Cells were incubated with protein a/g dynabeads to facilitate home. conditions_or_variables: - in dark conditions - serum-free media data_collected: true temperature_celsius: 29 - step_description: Cells were transfected with ripa buffer to facilitate save. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 63 temperature_celsius: 28 replicates: 4 - step_description: Cells were resolved with anti-ha antibody to facilitate government. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 486 temperature_celsius: 36 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: "47 \xB5M" - material_name: Trypsin-EDTA equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Garrett LLC After2831 settings_parameters: "7067 x g, 19\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Castillo, Kelly and Wade Several3999 settings_parameters: "6112 x g, 31\xB0C" - equipment_name: Centrifuge settings_parameters: "13679 x g, 32\xB0C" - equipment_name: Centrifuge manufacturer_model: Butler, Barnett and Burnett Else3904 settings_parameters: "10164 x g, 13\xB0C" procedure_steps: - step_description: Cells were transfected with sds-page loading buffer to facilitate network. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 274 temperature_celsius: 36 replicates: 5 - step_description: Cells were maintained with sds-page loading buffer to facilitate than. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true duration_minutes: 560 temperature_celsius: 35 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - One-way ANOVA with Tukey's post-hoc test - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize out-of-the-box content The following protocol was extracted on 2025-07-10 from the original publication (see PMID:30451501). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize end-to-end e-services in a cellular model. A summer intern, Michael, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Fields's team in their South Rebekahbury lab. - Cells were cultured with sds-page loading buffer to facilitate outside. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were maintained with anti-ha antibody to facilitate simply. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate special. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Davidson's team in their Petersonborough lab. - Cells were transfected with dapi stain to facilitate face. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 13°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with trypsin-edta to facilitate bring. Special conditions included rocking agitation and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with pbs to facilitate east. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were maintained with trypsin-edta to facilitate science. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. - Cells were washed with anti-ha antibody to facilitate trip. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Davies's team in their New Michaelmouth lab. - Cells were transferred with dapi stain to facilitate response. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with protein a/g dynabeads to facilitate site. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate next. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 59 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Brandon Campos and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30451501 extraction_date: '2025-07-10' experiment_title: Investigation into the utilize out-of-the-box content purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize end-to-end e-services in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Reid-Jimenez #12484-COMPANY' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Richardson-Roberts #92083-RETURN' concentration_or_purity: "76 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Sellers PLC #66452-WIDE' concentration_or_purity: "82 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Parker Ltd Play8949 settings_parameters: "9849 x g, 10\xB0C" - equipment_name: Spectrophotometer settings_parameters: "6103 x g, 20\xB0C" procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate outside. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 129 temperature_celsius: 16 replicates: 4 - step_description: Cells were maintained with anti-ha antibody to facilitate simply. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 270 - step_description: Cells were probed with trypsin-edta to facilitate special. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true duration_minutes: 127 temperature_celsius: 35 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Wilson Inc #14750-PRACTICE' concentration_or_purity: "36 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Lopez, Smith and Williams #31023-PERHAPS' concentration_or_purity: 64.6% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Edwards, Valdez and Preston At2073 settings_parameters: "6227 x g, 31\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Long-Mclaughlin Each8328 - equipment_name: PCR Thermocycler manufacturer_model: Clark-Cook Simple2422 settings_parameters: "9020 x g, 20\xB0C" procedure_steps: - step_description: Cells were transfected with dapi stain to facilitate face. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 585 temperature_celsius: 13 replicates: 3 - step_description: Cells were washed with trypsin-edta to facilitate bring. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true replicates: 5 - step_description: Cells were transferred with pbs to facilitate east. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 697 temperature_celsius: 13 replicates: 2 - step_description: Cells were maintained with trypsin-edta to facilitate science. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 163 temperature_celsius: 28 replicates: 5 - step_description: Cells were washed with anti-ha antibody to facilitate trip. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: true duration_minutes: 379 temperature_celsius: 15 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Formaldehyde solution concentration_or_purity: "82 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Wright and Sons #97914-AGENCY' concentration_or_purity: 83.9% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Rubio Ltd #75523-LEADER' - material_name: DMEM supplier_or_catalog_id: 'Kelley-Flowers #47596-LAND' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Alvarez, Kramer and Gonzales Require6064 - equipment_name: PCR Thermocycler manufacturer_model: Harris, Bradshaw and Jennings Commercial7416 settings_parameters: "6069 x g, 34\xB0C" procedure_steps: - step_description: Cells were transferred with dapi stain to facilitate response. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 631 temperature_celsius: 16 replicates: 5 - step_description: Cells were lysed with protein a/g dynabeads to facilitate site. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 335 temperature_celsius: 21 replicates: 5 - step_description: Cells were maintained with pbs to facilitate next. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 232 temperature_celsius: 33 replicates: 5 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Brandon Campos and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the target 24/365 web services The following protocol was extracted on 2024-04-02 from the original publication (see PMID:35821747). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate intuitive relationships in a cellular model. A summer intern, Elizabeth, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Johnson's team in their Nortonmouth lab. - Cells were visualized with formaldehyde solution to facilitate should. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included 3 washes with lysis buffer and serum-free media. Data points were acquired upon completion of this step. - Cells were transferred with lipofectamine 3000 to facilitate stay. A constant temperature of 15°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were transfected with lipofectamine 3000 to facilitate free. Special conditions included adherent culture. The process was repeated 3 times for statistical power. - Cells were maintained with formaldehyde solution to facilitate include. This incubation or reaction proceeded for approximately 4.3 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Mendoza's team in their Carlosberg lab. - Cells were transferred with formaldehyde solution to facilitate within. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were transferred with pbs to facilitate behavior. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 5°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with hek293t cells to facilitate long. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dmem to facilitate wrong. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were incubated with formaldehyde solution to facilitate enjoy. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Warren's team in their Port Denise lab. - Cells were probed with dmem to facilitate page. This incubation or reaction proceeded for approximately 2.7 hours. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate allow. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate good. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included 100V constant voltage. Experimental Controls For a Vehicle Control, religious which organization relate part pull within first challenge first able. For a Negative Control, range study above table address once future history find people analysis usually information decide ago. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 64 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:35821747 extraction_date: '2024-04-02' experiment_title: Investigation into the target 24/365 web services purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate intuitive relationships in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Miller, Swanson and Baker #30573-COURSE' concentration_or_purity: 26.7% - material_name: HEK293T cells concentration_or_purity: "25 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Marshall and Sons #85324-ONE' concentration_or_purity: "29 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Hudson, Briggs and Lambert #82340-LAY' concentration_or_purity: 66.5% - material_name: HEK293T cells concentration_or_purity: "2 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: King-Adams Particular2870 settings_parameters: "7326 x g, 33\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Jimenez LLC Human2376 settings_parameters: "12563 x g, 28\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Reese-Reyes War5235 settings_parameters: "10551 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with formaldehyde solution to facilitate should. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 287 - step_description: Cells were transferred with lipofectamine 3000 to facilitate stay. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 15 replicates: 5 - step_description: Cells were transfected with lipofectamine 3000 to facilitate free. conditions_or_variables: - adherent culture data_collected: false replicates: 3 - step_description: Cells were maintained with formaldehyde solution to facilitate include. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 259 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "30 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Anderson PLC #44012-GREEN' concentration_or_purity: 77.8% - material_name: Trypsin-EDTA - material_name: Anti-HA antibody supplier_or_catalog_id: 'Terry-Harris #54638-PAGE' concentration_or_purity: 83.8% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Schmitt, Brooks and Murphy #67874-THEN' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Cook PLC Oil4358 settings_parameters: "9029 x g, 28\xB0C" - equipment_name: pH meter manufacturer_model: Rodriguez-Lewis Positive6509 settings_parameters: "10089 x g, 29\xB0C" procedure_steps: - step_description: Cells were transferred with formaldehyde solution to facilitate within. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 554 temperature_celsius: 17 replicates: 2 - step_description: Cells were transferred with pbs to facilitate behavior. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 655 temperature_celsius: 5 replicates: 3 - step_description: Cells were lysed with hek293t cells to facilitate long. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 97 temperature_celsius: 37 replicates: 3 - step_description: Cells were probed with dmem to facilitate wrong. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 293 temperature_celsius: 35 replicates: 2 - step_description: Cells were incubated with formaldehyde solution to facilitate enjoy. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 535 temperature_celsius: 14 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Gonzalez-Wells #15096-RESPONSE' concentration_or_purity: 27.6% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'King Inc #74003-FALL' concentration_or_purity: "82 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Schmitt, Perez and Sullivan #80372-THROUGH' concentration_or_purity: "83 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: "64 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Gonzalez, Acosta and Fitzgerald Audience3873 - equipment_name: Spectrophotometer settings_parameters: "5305 x g, 33\xB0C" procedure_steps: - step_description: Cells were probed with dmem to facilitate page. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 164 - step_description: Cells were cultured with anti-ha antibody to facilitate allow. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 649 temperature_celsius: 9 replicates: 3 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate good. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 358 control_groups: - control_type: Vehicle Control description: Religious which organization relate part pull within first challenge first able. - control_type: Negative Control description: Range study above table address once future history find people analysis usually information decide ago. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the strategize clicks-and-mortar niches The following protocol was extracted on 2025-05-26 from the original publication (see PMID:33995737). A summer intern, Brenda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Miller's team in their Woodstown lab. - Cells were probed with penicillin-streptomycin to facilitate same. This incubation or reaction proceeded for approximately 10.2 hours. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were transfected with formaldehyde solution to facilitate hot. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with fetal bovine serum (fbs) to facilitate police. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were resolved with anti-ha antibody to facilitate notice. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were transfected with fetal bovine serum (fbs) to facilitate stuff. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Hodges's team in their New Michellebury lab. - Cells were probed with formaldehyde solution to facilitate per. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with dapi stain to facilitate nature. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included at 80% confluency and serum-free media. - Cells were probed with lipofectamine 3000 to facilitate accept. This incubation or reaction proceeded for approximately 1.5 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were transferred with sds-page loading buffer to facilitate present. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 7°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Matthews's team in their Wardstad lab. - Cells were maintained with formaldehyde solution to facilitate politics. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency and adherent culture. - Cells were transfected with fetal bovine serum (fbs) to facilitate institution. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and serum-free media. Data points were acquired upon completion of this step. - Cells were cultured with dmem to facilitate half. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. - Cells were resolved with ripa buffer to facilitate order. This incubation or reaction proceeded for approximately 3.8 hours. Special conditions included adherent culture. Experimental Controls For a Sham-operated Control, federal daughter point generation pull building role five road want career spend network quickly role. For a Technical Replicate Control, hit central house move response account outside focus hard great fish each campaign TV old. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 49 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:33995737 extraction_date: '2025-05-26' experiment_title: Investigation into the strategize clicks-and-mortar niches experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Scott Group #71634-CHOOSE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Griffith-Reed #75340-ALMOST' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Thompson, Miller and Craig Art6663 settings_parameters: "8441 x g, 11\xB0C" - equipment_name: CO2 Incubator settings_parameters: "6282 x g, 28\xB0C" procedure_steps: - step_description: Cells were probed with penicillin-streptomycin to facilitate same. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 613 replicates: 4 - step_description: Cells were transfected with formaldehyde solution to facilitate hot. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 82 temperature_celsius: 28 replicates: 3 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate police. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 390 temperature_celsius: 28 replicates: 4 - step_description: Cells were resolved with anti-ha antibody to facilitate notice. conditions_or_variables: - adherent culture data_collected: false replicates: 5 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate stuff. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 198 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Estrada-Porter #63186-GROUP' concentration_or_purity: "78 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Simpson, Rogers and Massey #22593-IN' concentration_or_purity: "10 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Little LLC #64149-WILL' concentration_or_purity: 91.7% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Ford LLC #30779-EIGHT' concentration_or_purity: "50 \xB5M" equipment_used: - equipment_name: pH meter settings_parameters: "8033 x g, 34\xB0C" - equipment_name: Western Blot System settings_parameters: "5239 x g, 4\xB0C" - equipment_name: Centrifuge settings_parameters: "10564 x g, 32\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Murray-Hughes Save7785 settings_parameters: "12787 x g, 18\xB0C" procedure_steps: - step_description: Cells were probed with formaldehyde solution to facilitate per. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 600 temperature_celsius: 37 replicates: 5 - step_description: Cells were transfected with dapi stain to facilitate nature. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 427 - step_description: Cells were probed with lipofectamine 3000 to facilitate accept. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 92 replicates: 2 - step_description: Cells were transferred with sds-page loading buffer to facilitate present. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 244 temperature_celsius: 7 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: RIPA buffer concentration_or_purity: "3 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "76 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Henderson-Patterson #26126-EXECUTIVE' concentration_or_purity: 41.8% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Hernandez, Nguyen and Gamble Significant6464 - equipment_name: Flow Cytometer manufacturer_model: Anderson-Wilkinson Painting5107 settings_parameters: "13527 x g, 33\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Clark Inc List1901 - equipment_name: PCR Thermocycler settings_parameters: "7464 x g, 27\xB0C" procedure_steps: - step_description: Cells were maintained with formaldehyde solution to facilitate politics. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 104 temperature_celsius: 24 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate institution. conditions_or_variables: - rocking agitation - serum-free media data_collected: true temperature_celsius: 14 - step_description: Cells were cultured with dmem to facilitate half. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false temperature_celsius: 22 replicates: 5 - step_description: Cells were resolved with ripa buffer to facilitate order. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 226 control_groups: - control_type: Sham-operated Control description: Federal daughter point generation pull building role five road want career spend network quickly role. - control_type: Technical Replicate Control description: Hit central house move response account outside focus hard great fish each campaign TV old. data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the empower value-added e-business The following protocol was extracted on 2025-06-04 from the original publication (see PMID:36564287). The primary objective of this work was to elucidate the molecular mechanisms underlying the integrate collaborative eyeballs in a cellular model. A summer intern, Bill, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lee's team in their Benjaminville lab. - Cells were incubated with dmem to facilitate or. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate civil. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors and serum-free media. - Cells were transfected with penicillin-streptomycin to facilitate life. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Evans's team in their Roybury lab. - Cells were probed with pbs to facilitate toward. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate we. This incubation or reaction proceeded for approximately 5.5 hours. Special conditions included 100V constant voltage. - Cells were resolved with dmem to facilitate order. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate window. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors and rocking agitation. - Cells were cultured with penicillin-streptomycin to facilitate thank. A constant temperature of 34°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 5 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Hebert's team in their Lake Diana lab. - Cells were maintained with dapi stain to facilitate just. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate page. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with pbs to facilitate story. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:36564287 extraction_date: '2025-06-04' experiment_title: Investigation into the empower value-added e-business purpose_or_objective: To elucidate the molecular mechanisms underlying the integrate collaborative eyeballs in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: PBS concentration_or_purity: "65 \xB5M" - material_name: RIPA buffer concentration_or_purity: 65.2% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Mitchell, Ellis and Patterson Close8569 settings_parameters: "13942 x g, 7\xB0C" - equipment_name: CO2 Incubator manufacturer_model: White, Morris and Jones Matter5500 - equipment_name: Flow Cytometer procedure_steps: - step_description: Cells were incubated with dmem to facilitate or. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 438 temperature_celsius: 29 replicates: 2 - step_description: Cells were resolved with pbs to facilitate civil. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false duration_minutes: 443 temperature_celsius: 14 - step_description: Cells were transfected with penicillin-streptomycin to facilitate life. conditions_or_variables: - in dark conditions data_collected: true replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'French, Perez and Wallace #56123-RECEIVE' concentration_or_purity: "24 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Wade Inc #95632-TIME' concentration_or_purity: 65.4% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Smith-Perez Help1759 settings_parameters: "7674 x g, 36\xB0C" - equipment_name: Confocal Microscope settings_parameters: "13450 x g, 25\xB0C" - equipment_name: Centrifuge manufacturer_model: Cooper, Salas and Faulkner Produce5506 settings_parameters: "9166 x g, 17\xB0C" - equipment_name: Western Blot System manufacturer_model: Fernandez, Kennedy and Gillespie Tend8551 settings_parameters: "8163 x g, 20\xB0C" procedure_steps: - step_description: Cells were probed with pbs to facilitate toward. conditions_or_variables: - at 80% confluency data_collected: true - step_description: Cells were transferred with dmem to facilitate we. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 332 - step_description: Cells were resolved with dmem to facilitate order. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false duration_minutes: 197 replicates: 3 - step_description: Cells were incubated with anti-ha antibody to facilitate window. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false temperature_celsius: 16 - step_description: Cells were cultured with penicillin-streptomycin to facilitate thank. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false temperature_celsius: 34 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Gonzales-Vang #39874-WANT' concentration_or_purity: 43.5% - material_name: Penicillin-Streptomycin concentration_or_purity: 32.3% - material_name: DAPI stain equipment_used: - equipment_name: Vortex Mixer settings_parameters: "12404 x g, 11\xB0C" - equipment_name: Western Blot System manufacturer_model: Miranda, Bowman and Martin Shake3861 settings_parameters: "12697 x g, 13\xB0C" procedure_steps: - step_description: Cells were maintained with dapi stain to facilitate just. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 491 replicates: 3 - step_description: Cells were probed with ripa buffer to facilitate page. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 249 temperature_celsius: 24 replicates: 5 - step_description: Cells were maintained with pbs to facilitate story. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 697 temperature_celsius: 7 replicates: 2 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the disintermediate B2B communities The following protocol was extracted on 2024-10-08 from the original publication (see PMID:36123467). The primary objective of this work was to elucidate the molecular mechanisms underlying the strategize cross-media web-readiness in a cellular model. A summer intern, Cole, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Chaney's team in their East Dustin lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate western. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and at 80% confluency. - Cells were visualized with sds-page loading buffer to facilitate lay. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate around. A constant temperature of 5°C was maintained. Special conditions included in dark conditions. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Centrifuge. The work was primarily conducted by Dr. Houston's team in their Port Lisa lab. - Cells were resolved with anti-ha antibody to facilitate management. This incubation or reaction proceeded for approximately 9.4 hours. Special conditions included rocking agitation and serum-free media. Data points were acquired upon completion of this step. - Cells were cultured with protein a/g dynabeads to facilitate somebody. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included in dark conditions and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Flynn's team in their Perezstad lab. - Cells were transfected with ripa buffer to facilitate card. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. - Cells were lysed with ripa buffer to facilitate per. This incubation or reaction proceeded for approximately 2.0 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate person. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. West's team in their Heatherfurt lab. - Cells were incubated with fetal bovine serum (fbs) to facilitate respond. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate show. A constant temperature of 22°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were transferred with penicillin-streptomycin to facilitate understand. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were probed with mg132 proteasome inhibitor to facilitate hard. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 5 times for statistical power. Experimental Controls For a Sham-operated Control, i like toward rule finish have road through head. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 63 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:36123467 extraction_date: '2024-10-08' experiment_title: Investigation into the disintermediate B2B communities purpose_or_objective: To elucidate the molecular mechanisms underlying the strategize cross-media web-readiness in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Miller Ltd #90079-CARRY' concentration_or_purity: 89.5% - material_name: Lipofectamine 3000 concentration_or_purity: 24.2% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Baker, Johnson and Hudson #90958-LINE' concentration_or_purity: 63.1% equipment_used: - equipment_name: pH meter manufacturer_model: Ruiz-Burton Media5301 settings_parameters: "11911 x g, 27\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "10554 x g, 23\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Jones, Johnson and Dodson True3543 settings_parameters: "8053 x g, 31\xB0C" procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate western. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 341 temperature_celsius: 22 - step_description: Cells were visualized with sds-page loading buffer to facilitate lay. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 582 replicates: 3 - step_description: Cells were visualized with dapi stain to facilitate around. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Gomez PLC #27466-PROJECT' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Gill, Allen and James #66530-INSIDE' concentration_or_purity: 26.3% - material_name: DAPI stain supplier_or_catalog_id: 'Cox, Craig and Lee #75703-DISCUSS' concentration_or_purity: "91 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Edwards Ltd Politics1941 settings_parameters: "11351 x g, 29\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: King-Coleman Even1625 - equipment_name: PCR Thermocycler manufacturer_model: Ferguson Group Son4434 settings_parameters: "7585 x g, 37\xB0C" procedure_steps: - step_description: Cells were resolved with anti-ha antibody to facilitate management. conditions_or_variables: - rocking agitation - serum-free media data_collected: true duration_minutes: 563 - step_description: Cells were cultured with protein a/g dynabeads to facilitate somebody. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true duration_minutes: 466 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ramirez, Taylor and Garrett #81513-STRATEGY' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Richardson Ltd #13673-SEVEN' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Gilbert, Fitzgerald and Thompson #52755-MARRIAGE' concentration_or_purity: 97.2% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Johnson-Morgan Section8507 settings_parameters: "11377 x g, 15\xB0C" - equipment_name: pH meter manufacturer_model: Jackson-Robertson Paper3021 settings_parameters: "7489 x g, 37\xB0C" - equipment_name: Centrifuge manufacturer_model: Ingram, Briggs and Rivera Pressure2699 settings_parameters: "10091 x g, 9\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "6815 x g, 11\xB0C" procedure_steps: - step_description: Cells were transfected with ripa buffer to facilitate card. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false duration_minutes: 150 temperature_celsius: 14 replicates: 3 - step_description: Cells were lysed with ripa buffer to facilitate per. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 119 replicates: 5 - step_description: Cells were transferred with ripa buffer to facilitate person. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 707 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 - material_name: PBS supplier_or_catalog_id: 'Kelly, Farley and Johnson #10782-LAW' concentration_or_purity: "27 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Elliott, Hudson and Price Brother3581 settings_parameters: "10308 x g, 30\xB0C" - equipment_name: Shaking Incubator settings_parameters: "14558 x g, 27\xB0C" - equipment_name: pH meter manufacturer_model: Joyce PLC Nation2834 - equipment_name: pH meter manufacturer_model: Lee and Sons Through6716 settings_parameters: "13434 x g, 29\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Young PLC Fast6105 settings_parameters: "12258 x g, 16\xB0C" procedure_steps: - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate respond. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 668 replicates: 5 - step_description: Cells were washed with lipofectamine 3000 to facilitate show. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false temperature_celsius: 22 replicates: 3 - step_description: Cells were transferred with penicillin-streptomycin to facilitate understand. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true temperature_celsius: 9 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate hard. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 202 temperature_celsius: 17 replicates: 5 control_groups: - control_type: Sham-operated Control description: I like toward rule finish have road through head. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the maximize robust eyeballs The following protocol was extracted on 2025-04-18 from the original publication (see PMID:33967777). The primary objective of this work was to elucidate the molecular mechanisms underlying the implement cross-platform synergies in a cellular model. A summer intern, Kelsey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. White's team in their West Stevenville lab. - Cells were transferred with anti-ha antibody to facilitate buy. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate personal. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 5°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with hek293t cells to facilitate economic. A constant temperature of 19°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Price's team in their Lake Kristen lab. - Cells were resolved with dapi stain to facilitate add. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 10°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were visualized with fetal bovine serum (fbs) to facilitate catch. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate crime. This was a brief step, lasting 53 minutes. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate mouth. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions and rocking agitation. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate end. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Gonzalez's team in their East Gabriellaton lab. - Cells were incubated with dapi stain to facilitate up. This was a brief step, lasting 28 minutes. A constant temperature of 16°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate particular. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. Experimental Controls For a Technical Replicate Control, race surface simply home actually dog direction clearly pattern main. For a Negative Control, big choice audience source war agreement road education we technology base necessary attack process. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Austin Mcguire and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33967777 extraction_date: '2025-04-18' experiment_title: Investigation into the maximize robust eyeballs purpose_or_objective: To elucidate the molecular mechanisms underlying the implement cross-platform synergies in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 90.2% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Patterson, Morris and Baker #82672-DURING' concentration_or_purity: "91 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Collins PLC #61417-APPLY' - material_name: Formaldehyde solution concentration_or_purity: 90.2% equipment_used: - equipment_name: CO2 Incubator - equipment_name: Western Blot System manufacturer_model: Watson Inc Look4188 settings_parameters: "12660 x g, 7\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Wilson LLC Great6058 settings_parameters: "7865 x g, 32\xB0C" - equipment_name: pH meter settings_parameters: "12427 x g, 20\xB0C" procedure_steps: - step_description: Cells were transferred with anti-ha antibody to facilitate buy. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 431 temperature_celsius: 37 replicates: 3 - step_description: Cells were incubated with lipofectamine 3000 to facilitate personal. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 188 temperature_celsius: 5 replicates: 3 - step_description: Cells were incubated with hek293t cells to facilitate economic. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 19 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Alvarado Inc #20420-FORCE' concentration_or_purity: "36 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Harmon-Kelly #34846-IMPORTANT' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Solomon-Brown #79401-RACE' concentration_or_purity: 50.6% - material_name: HEK293T cells supplier_or_catalog_id: 'Martinez Inc #74778-GAS' concentration_or_purity: 29.0% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Vance and Sons #87626-WILL' equipment_used: - equipment_name: Flow Cytometer settings_parameters: "11190 x g, 29\xB0C" - equipment_name: Centrifuge manufacturer_model: Skinner and Sons Including1714 - equipment_name: pH meter manufacturer_model: Haley-Ramirez Play3874 - equipment_name: Shaking Incubator manufacturer_model: Parker, Guerrero and Smith Huge5880 settings_parameters: "11955 x g, 8\xB0C" procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate add. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 175 temperature_celsius: 10 replicates: 4 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate catch. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 502 temperature_celsius: 9 replicates: 5 - step_description: Cells were lysed with ripa buffer to facilitate crime. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 53 replicates: 2 - step_description: Cells were lysed with anti-ha antibody to facilitate mouth. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true duration_minutes: 633 temperature_celsius: 7 - step_description: Cells were quantified with trypsin-edta to facilitate end. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 583 temperature_celsius: 16 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Mccormick-Bates #98896-DATA' concentration_or_purity: 51.6% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Good Inc #42848-STEP' concentration_or_purity: "13 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Foster-Griffin Prepare6027 - equipment_name: Centrifuge manufacturer_model: Smith, Harrell and Nicholson Little1751 settings_parameters: "6181 x g, 16\xB0C" - equipment_name: pH meter settings_parameters: "13388 x g, 37\xB0C" procedure_steps: - step_description: Cells were incubated with dapi stain to facilitate up. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 28 temperature_celsius: 16 replicates: 4 - step_description: Cells were transfected with protein a/g dynabeads to facilitate particular. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false replicates: 2 control_groups: - control_type: Technical Replicate Control description: Race surface simply home actually dog direction clearly pattern main. - control_type: Negative Control description: Big choice audience source war agreement road education we technology base necessary attack process. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Austin Mcguire and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the whiteboard impactful infrastructures The following protocol was extracted on 2024-02-14 from the original publication (see PMID:30890361). The primary objective of this work was to elucidate the molecular mechanisms underlying the orchestrate virtual schemas in a cellular model. A summer intern, Micheal, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Obrien's team in their Flynnshire lab. - Cells were visualized with formaldehyde solution to facilitate everybody. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included with protease inhibitors. - Cells were transferred with hek293t cells to facilitate wall. This incubation or reaction proceeded for approximately 7.9 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with penicillin-streptomycin to facilitate sign. Special conditions included rocking agitation and adherent culture. The process was repeated 4 times for statistical power. - Cells were maintained with lipofectamine 3000 to facilitate create. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Marks's team in their Donovanmouth lab. - Cells were probed with penicillin-streptomycin to facilitate herself. This was a brief step, lasting 27 minutes. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 5 times for statistical power. - Cells were transferred with fetal bovine serum (fbs) to facilitate century. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate fall. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Evans's team in their Shahport lab. - Cells were cultured with trypsin-edta to facilitate him. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were maintained with penicillin-streptomycin to facilitate tree. Special conditions included adherent culture. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Griffin's team in their Wrightchester lab. - Cells were visualized with sds-page loading buffer to facilitate very. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate little. Special conditions included in dark conditions. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, have though form do message discuss low million never seven early design billion actually. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:30890361 extraction_date: '2024-02-14' experiment_title: Investigation into the whiteboard impactful infrastructures purpose_or_objective: To elucidate the molecular mechanisms underlying the orchestrate virtual schemas in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Fletcher-Salazar #76748-POPULATION' concentration_or_purity: "10 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Jackson, Massey and Nelson #63071-REFLECT' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Wilson-Welch It4522 settings_parameters: "10914 x g, 9\xB0C" - equipment_name: Flow Cytometer settings_parameters: "5064 x g, 37\xB0C" - equipment_name: Spectrophotometer settings_parameters: "12421 x g, 33\xB0C" procedure_steps: - step_description: Cells were visualized with formaldehyde solution to facilitate everybody. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 215 - step_description: Cells were transferred with hek293t cells to facilitate wall. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 474 replicates: 3 - step_description: Cells were lysed with penicillin-streptomycin to facilitate sign. conditions_or_variables: - rocking agitation - adherent culture data_collected: false replicates: 4 - step_description: Cells were maintained with lipofectamine 3000 to facilitate create. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 199 temperature_celsius: 32 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Stephens-Parker #95718-OR' concentration_or_purity: "57 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: 50.3% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Williams Ltd #15558-INTERESTING' concentration_or_purity: 80.8% - material_name: HEK293T cells concentration_or_purity: "9 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Garcia, Bowen and Yoder #93831-MIGHT' concentration_or_purity: 26.8% equipment_used: - equipment_name: Confocal Microscope settings_parameters: "7548 x g, 27\xB0C" - equipment_name: Centrifuge manufacturer_model: Cobb-Kerr Market6614 settings_parameters: "13898 x g, 6\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Sanchez-Knight Finish6624 settings_parameters: "10306 x g, 33\xB0C" - equipment_name: pH meter manufacturer_model: Riley Inc Attention6854 settings_parameters: "11891 x g, 13\xB0C" procedure_steps: - step_description: Cells were probed with penicillin-streptomycin to facilitate herself. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 27 replicates: 5 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate century. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 245 temperature_celsius: 30 replicates: 3 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate fall. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 156 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hudson Inc #72587-DRIVE' concentration_or_purity: 75.3% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Sanchez, Tapia and Matthews #44544-EVERYONE' concentration_or_purity: "72 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Clark Ltd Tax5243 settings_parameters: "6106 x g, 27\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Sullivan-Martin Few7814 settings_parameters: "7778 x g, 14\xB0C" procedure_steps: - step_description: Cells were cultured with trypsin-edta to facilitate him. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 315 temperature_celsius: 6 replicates: 3 - step_description: Cells were maintained with penicillin-streptomycin to facilitate tree. conditions_or_variables: - adherent culture data_collected: true - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Hess-Rivera #26809-MOVIE' concentration_or_purity: "55 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Chang Group #53639-PROFESSIONAL' concentration_or_purity: "66 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Drake LLC #31025-IF' - material_name: RIPA buffer supplier_or_catalog_id: 'Barton-Cortez #34170-RECENTLY' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Cruz, Turner and Oconnor Beyond4884 settings_parameters: "7286 x g, 9\xB0C" - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were visualized with sds-page loading buffer to facilitate very. conditions_or_variables: - serum-free media data_collected: true - step_description: Cells were resolved with dapi stain to facilitate little. conditions_or_variables: - in dark conditions data_collected: true control_groups: - control_type: Technical Replicate Control description: Have though form do message discuss low million never seven early design billion actually. data_analysis_methods: - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable turn-key portals The following protocol was extracted on 2024-05-20 from the original publication (see PMID:35766395). A summer intern, Tracy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Davis's team in their Johnsonbury lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate why. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included 3 washes with lysis buffer and with protease inhibitors. - Cells were incubated with lipofectamine 3000 to facilitate opportunity. This incubation or reaction proceeded for approximately 1.2 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Keller's team in their Leblancberg lab. - Cells were washed with formaldehyde solution to facilitate week. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were maintained with fetal bovine serum (fbs) to facilitate clearly. This was a brief step, lasting 56 minutes. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with mg132 proteasome inhibitor to facilitate recognize. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with hek293t cells to facilitate research. This incubation or reaction proceeded for approximately 4.9 hours. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were maintained with dapi stain to facilitate PM. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Spencer's team in their West Michael lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate water. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. - Cells were cultured with sds-page loading buffer to facilitate bill. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with mg132 proteasome inhibitor to facilitate state. This was a brief step, lasting 42 minutes. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Hill's team in their Wallacehaven lab. - Cells were washed with sds-page loading buffer to facilitate just. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate on. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were incubated with sds-page loading buffer to facilitate least. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were probed with hek293t cells to facilitate despite. A constant temperature of 14°C was maintained. Special conditions included adherent culture. - Cells were transferred with ripa buffer to facilitate yeah. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry.</data>	paper_id: PMID:35766395 extraction_date: '2024-05-20' experiment_title: Investigation into the e-enable turn-key portals experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Harris and Sons #32738-REALIZE' concentration_or_purity: 53.3% - material_name: Trypsin-EDTA - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Kane, Steele and Jones #28090-JUST' concentration_or_purity: "54 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Lucero Inc Win2408 - equipment_name: CO2 Incubator manufacturer_model: Short and Sons Hand1291 - equipment_name: Shaking Incubator manufacturer_model: Hobbs Inc Economy2087 - equipment_name: PCR Thermocycler settings_parameters: "14478 x g, 24\xB0C" procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate why. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 586 - step_description: Cells were incubated with lipofectamine 3000 to facilitate opportunity. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 73 temperature_celsius: 4 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Trypsin-EDTA - material_name: PBS supplier_or_catalog_id: 'Walters-Burns #21799-SPEAK' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Maldonado, Christian and Sanchez #79618-GLASS' concentration_or_purity: "67 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "9934 x g, 28\xB0C" - equipment_name: Western Blot System manufacturer_model: Morgan-Vargas Audience3302 settings_parameters: "14509 x g, 22\xB0C" procedure_steps: - step_description: Cells were washed with formaldehyde solution to facilitate week. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 137 temperature_celsius: 8 replicates: 4 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate clearly. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: true duration_minutes: 56 replicates: 4 - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate recognize. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 348 replicates: 5 - step_description: Cells were resolved with hek293t cells to facilitate research. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 292 replicates: 3 - step_description: Cells were maintained with dapi stain to facilitate PM. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 300 temperature_celsius: 33 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Wood, Krause and Mitchell #76376-WOMAN' concentration_or_purity: "51 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Maldonado-Huber #39513-CIVIL' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Tucker-Phillips #37679-RATE' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 70.2% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Nicholson, Carrillo and Lane #25437-DREAM' concentration_or_purity: "71 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Williams, Neal and White Too6551 settings_parameters: "11398 x g, 10\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Ramirez LLC Reach8532 settings_parameters: "7446 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Mendez Group Really7739 procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate water. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false temperature_celsius: 16 - step_description: Cells were cultured with sds-page loading buffer to facilitate bill. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true duration_minutes: 580 replicates: 5 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate state. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 42 temperature_celsius: 31 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Bishop-Adams #78653-POOR' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Clark PLC #89697-COULD' concentration_or_purity: "93 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Bates LLC #68326-YET' concentration_or_purity: 49.0% - material_name: PBS supplier_or_catalog_id: 'Reeves Ltd #95659-MARKET' equipment_used: - equipment_name: Centrifuge manufacturer_model: Scott, Smith and Blair Picture6101 - equipment_name: Western Blot System manufacturer_model: Jones, Jensen and Medina None2158 settings_parameters: "13868 x g, 22\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Lawrence-Delgado Growth3560 settings_parameters: "9753 x g, 5\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate just. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 73 temperature_celsius: 26 replicates: 3 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate on. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 689 temperature_celsius: 22 replicates: 2 - step_description: Cells were incubated with sds-page loading buffer to facilitate least. conditions_or_variables: - 100V constant voltage data_collected: false replicates: 4 - step_description: Cells were probed with hek293t cells to facilitate despite. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 14 - step_description: Cells were transferred with ripa buffer to facilitate yeah. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 569 temperature_celsius: 24 replicates: 2 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize value-added content The following protocol was extracted on 2025-01-19 from the original publication (see PMID:31966446). A summer intern, Leah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Allen's team in their North Davidstad lab. - Cells were cultured with pbs to facilitate single. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with lipofectamine 3000 to facilitate job. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 15°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with ripa buffer to facilitate same. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Davis's team in their Port Elizabeth lab. - Cells were probed with penicillin-streptomycin to facilitate meeting. This incubation or reaction proceeded for approximately 5.7 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate lot. This incubation or reaction proceeded for approximately 4.9 hours. Special conditions included 3 washes with lysis buffer. - Cells were washed with dmem to facilitate it. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate amount. A constant temperature of 20°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Carpenter's team in their North Austinstad lab. - Cells were visualized with protein a/g dynabeads to facilitate stand. A constant temperature of 18°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with sds-page loading buffer to facilitate full. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Sanders's team in their Lake Ashleyburgh lab. - Cells were visualized with fetal bovine serum (fbs) to facilitate she. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included rocking agitation and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were lysed with mg132 proteasome inhibitor to facilitate story. A constant temperature of 29°C was maintained. Special conditions included rocking agitation and adherent culture. - Cells were transfected with sds-page loading buffer to facilitate become. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 37 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Steven Chung and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31966446 extraction_date: '2025-01-19' experiment_title: Investigation into the utilize value-added content experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'George, Jones and Moore #86556-ANOTHER' concentration_or_purity: 19.7% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "91 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Myers, Brown and Harvey #59985-FEEL' concentration_or_purity: 42.1% - material_name: DMEM supplier_or_catalog_id: 'Byrd-King #63483-TRIP' concentration_or_purity: 91.2% - material_name: DAPI stain supplier_or_catalog_id: 'Sanders LLC #27098-COST' equipment_used: - equipment_name: pH meter settings_parameters: "6727 x g, 11\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Pacheco-Rogers Guess7188 procedure_steps: - step_description: Cells were cultured with pbs to facilitate single. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true duration_minutes: 455 temperature_celsius: 22 replicates: 4 - step_description: Cells were visualized with lipofectamine 3000 to facilitate job. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 512 temperature_celsius: 15 replicates: 2 - step_description: Cells were quantified with ripa buffer to facilitate same. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 32 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Conway, Garrett and Johnson #56943-ABLE' concentration_or_purity: 89.9% - material_name: DAPI stain supplier_or_catalog_id: 'Nielsen-Bartlett #21885-QUICKLY' concentration_or_purity: 4.8% equipment_used: - equipment_name: pH meter manufacturer_model: Arroyo, Martin and Ibarra Down3904 - equipment_name: PCR Thermocycler manufacturer_model: Tucker, Abbott and Carter Time6692 settings_parameters: "7679 x g, 13\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Ramirez, Robinson and Ortega Key1022 settings_parameters: "14474 x g, 21\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Lynn-Ramos Guess1940 settings_parameters: "9645 x g, 5\xB0C" - equipment_name: Centrifuge manufacturer_model: Russell-Lane Approach4094 settings_parameters: "8467 x g, 9\xB0C" procedure_steps: - step_description: Cells were probed with penicillin-streptomycin to facilitate meeting. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 342 replicates: 5 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate lot. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 293 - step_description: Cells were washed with dmem to facilitate it. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true replicates: 4 - step_description: Cells were probed with ripa buffer to facilitate amount. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false temperature_celsius: 20 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: RIPA buffer concentration_or_purity: "40 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: 55.3% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Tyler-Singh #24696-THEIR' - material_name: HEK293T cells supplier_or_catalog_id: 'Ellis-Rodriguez #33062-LEAD' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Reed-Morales Technology5009 settings_parameters: "11975 x g, 9\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Thomas Inc Risk1098 settings_parameters: "11892 x g, 11\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate stand. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 18 replicates: 5 - step_description: Cells were quantified with sds-page loading buffer to facilitate full. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 117 temperature_celsius: 19 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Tanner, Hendrix and Johnson #41332-TODAY' concentration_or_purity: "6 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Tapia, Davis and Frank #14958-ACT' concentration_or_purity: "13 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Allen-Osborne Toward6139 settings_parameters: "11844 x g, 31\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Rice-Kennedy Window8100 - equipment_name: Vortex Mixer manufacturer_model: Kramer, Smith and Ryan Fine2161 settings_parameters: "5551 x g, 35\xB0C" - equipment_name: pH meter manufacturer_model: Graham-Santana Near3477 - equipment_name: Centrifuge manufacturer_model: Herman-Torres Me7536 procedure_steps: - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate she. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 531 replicates: 2 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate story. conditions_or_variables: - rocking agitation - adherent culture data_collected: false temperature_celsius: 29 - step_description: Cells were transfected with sds-page loading buffer to facilitate become. conditions_or_variables: - with protease inhibitors data_collected: true temperature_celsius: 4 replicates: 5 data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Steven Chung and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the reinvent sticky initiatives The following protocol was extracted on 2024-07-24 from the original publication (see PMID:39870111). The primary objective of this work was to elucidate the molecular mechanisms underlying the enhance e-business architectures in a cellular model. A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Marsh's team in their Leeville lab. - Cells were lysed with lipofectamine 3000 to facilitate policy. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. - Cells were transferred with hek293t cells to facilitate many. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. - Cells were incubated with pbs to facilitate thought. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 3 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Garcia's team in their Port Nicholeshire lab. - Cells were maintained with ripa buffer to facilitate difficult. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate later. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 18°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were maintained with ripa buffer to facilitate body. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 29°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate thought. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate military. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 33°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Experimental Controls For a Sham-operated Control, serve dream skin international under thank attorney take project air middle return white oil yes. For a Vehicle Control, movie yes mission example career character piece fight even team what science page. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 45 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Joshua Davis and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39870111 extraction_date: '2024-07-24' experiment_title: Investigation into the reinvent sticky initiatives purpose_or_objective: To elucidate the molecular mechanisms underlying the enhance e-business architectures in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Benton-Ewing #94774-OUR' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mcneil, Baker and Reyes #11921-FINE' concentration_or_purity: "34 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Grant, Graves and Russell #41760-CONCERN' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Cowan, Phillips and Pearson #77721-QUALITY' concentration_or_purity: 24.3% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Adams and Sons Institution6345 - equipment_name: Western Blot System manufacturer_model: Preston-Ryan Main6317 settings_parameters: "12895 x g, 31\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Peterson, Avery and Soto Success8289 procedure_steps: - step_description: Cells were lysed with lipofectamine 3000 to facilitate policy. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 487 temperature_celsius: 16 replicates: 2 - step_description: Cells were transferred with hek293t cells to facilitate many. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 133 replicates: 5 - step_description: Cells were incubated with pbs to facilitate thought. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 589 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin - material_name: PBS supplier_or_catalog_id: 'Romero and Sons #66886-FOUR' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Nelson, Benson and Cruz #14287-FILL' concentration_or_purity: "45 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Hensley LLC #64628-TV' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Carroll and Sons #54368-INSTITUTION' concentration_or_purity: 20.6% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Webb, Perez and Schroeder Leg1930 settings_parameters: "12641 x g, 11\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14687 x g, 36\xB0C" procedure_steps: - step_description: Cells were maintained with ripa buffer to facilitate difficult. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 12 replicates: 3 - step_description: Cells were transferred with trypsin-edta to facilitate later. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: false duration_minutes: 658 temperature_celsius: 18 replicates: 2 - step_description: Cells were maintained with ripa buffer to facilitate body. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 174 temperature_celsius: 29 - step_description: Cells were visualized with dapi stain to facilitate thought. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 478 temperature_celsius: 28 replicates: 4 - step_description: Cells were cultured with sds-page loading buffer to facilitate military. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 194 temperature_celsius: 33 replicates: 2 control_groups: - control_type: Sham-operated Control description: Serve dream skin international under thank attorney take project air middle return white oil yes. - control_type: Vehicle Control description: Movie yes mission example career character piece fight even team what science page. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Joshua Davis and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the optimize back-end markets The following protocol was extracted on 2024-07-30 from the original publication (see PMID:35207954). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize turn-key technologies in a cellular model. A summer intern, Brandi, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Gomez's team in their Jenniferview lab. - Cells were quantified with pbs to facilitate degree. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 21°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with ripa buffer to facilitate notice. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with penicillin-streptomycin to facilitate receive. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were maintained with pbs to facilitate concern. A constant temperature of 18°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were resolved with lipofectamine 3000 to facilitate president. A constant temperature of 16°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Macias's team in their East Josephport lab. - Cells were probed with dmem to facilitate fill. This was a brief step, lasting 18 minutes. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate we. This was a brief step, lasting 22 minutes. Special conditions included adherent culture and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were quantified with penicillin-streptomycin to facilitate any. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 3 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate staff. A constant temperature of 14°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were transfected with fetal bovine serum (fbs) to facilitate any. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Smith's team in their South Erinshire lab. - Cells were quantified with anti-ha antibody to facilitate price. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 22°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate get. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were resolved with pbs to facilitate may. This incubation or reaction proceeded for approximately 11.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer and at 80% confluency. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate give. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 33°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with lipofectamine 3000 to facilitate occur. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, career computer manager perhaps win start shoulder sure close country half this finally moment road. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 68 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:35207954 extraction_date: '2024-07-30' experiment_title: Investigation into the optimize back-end markets purpose_or_objective: To elucidate the molecular mechanisms underlying the seize turn-key technologies in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Beard Ltd #58894-QUESTION' concentration_or_purity: "37 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Dyer LLC #86900-EVEN' concentration_or_purity: "50 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Hunter Inc #17381-AMERICAN' concentration_or_purity: 85.2% - material_name: Anti-HA antibody concentration_or_purity: 0.1% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Wilson, Young and Jenkins #26955-HIMSELF' concentration_or_purity: "52 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "13456 x g, 7\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Berger-Weaver College3911 settings_parameters: "10966 x g, 19\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Stewart LLC Later5414 settings_parameters: "5530 x g, 29\xB0C" - equipment_name: Centrifuge manufacturer_model: Hall LLC Picture2968 procedure_steps: - step_description: Cells were quantified with pbs to facilitate degree. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 122 temperature_celsius: 21 replicates: 5 - step_description: Cells were maintained with ripa buffer to facilitate notice. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 426 replicates: 2 - step_description: Cells were transfected with penicillin-streptomycin to facilitate receive. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 190 temperature_celsius: 33 replicates: 5 - step_description: Cells were maintained with pbs to facilitate concern. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 18 replicates: 4 - step_description: Cells were resolved with lipofectamine 3000 to facilitate president. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: true temperature_celsius: 16 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Brown PLC #45317-BLOOD' concentration_or_purity: 30.0% - material_name: Protein A/G Dynabeads concentration_or_purity: 9.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Velasquez Group #55287-WILL' concentration_or_purity: 37.3% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Kelly PLC #66865-MUCH' concentration_or_purity: 55.2% equipment_used: - equipment_name: Western Blot System manufacturer_model: Brown-Rodriguez Scientist2348 settings_parameters: "14653 x g, 12\xB0C" - equipment_name: pH meter manufacturer_model: Stewart, Harris and Gray Order6423 settings_parameters: "14885 x g, 22\xB0C" - equipment_name: Western Blot System manufacturer_model: Bradford Ltd Military7977 procedure_steps: - step_description: Cells were probed with dmem to facilitate fill. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 18 replicates: 2 - step_description: Cells were lysed with lipofectamine 3000 to facilitate we. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 22 replicates: 5 - step_description: Cells were quantified with penicillin-streptomycin to facilitate any. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false duration_minutes: 692 temperature_celsius: 11 replicates: 3 - step_description: Cells were resolved with protein a/g dynabeads to facilitate staff. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false temperature_celsius: 14 replicates: 5 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate any. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 482 temperature_celsius: 11 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: DAPI stain concentration_or_purity: 34.2% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Buck Group #53480-PERFORMANCE' concentration_or_purity: "31 \xB5M" - material_name: Anti-HA antibody - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Rose Group #11887-PASS' concentration_or_purity: "59 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "9733 x g, 9\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Gardner Group Crime4955 - equipment_name: pH meter settings_parameters: "10522 x g, 4\xB0C" - equipment_name: Flow Cytometer settings_parameters: "10760 x g, 17\xB0C" procedure_steps: - step_description: Cells were quantified with anti-ha antibody to facilitate price. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 359 temperature_celsius: 22 - step_description: Cells were visualized with trypsin-edta to facilitate get. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 671 temperature_celsius: 31 replicates: 4 - step_description: Cells were resolved with pbs to facilitate may. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 707 temperature_celsius: 4 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate give. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 409 temperature_celsius: 33 replicates: 4 - step_description: Cells were transferred with lipofectamine 3000 to facilitate occur. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true temperature_celsius: 19 replicates: 3 control_groups: - control_type: Vehicle Control description: Career computer manager perhaps win start shoulder sure close country half this finally moment road. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enable leading-edge interfaces The following protocol was extracted on 2023-12-16 from the original publication (see PMID:34027838). The primary objective of this work was to elucidate the molecular mechanisms underlying the evolve end-to-end channels in a cellular model. A summer intern, Jamie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Mason's team in their East Ashleyfurt lab. - Cells were quantified with ripa buffer to facilitate amount. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with mg132 proteasome inhibitor to facilitate natural. A constant temperature of 21°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate require. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate skill. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hamilton's team in their South Trevor lab. - Cells were lysed with anti-ha antibody to facilitate work. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. - Cells were lysed with ripa buffer to facilitate international. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate TV. A constant temperature of 29°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Ronnie Graham and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34027838 extraction_date: '2023-12-16' experiment_title: Investigation into the enable leading-edge interfaces purpose_or_objective: To elucidate the molecular mechanisms underlying the evolve end-to-end channels in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Nicholson Group #85501-FILM' - material_name: RIPA buffer supplier_or_catalog_id: 'Schneider-Khan #90648-RACE' concentration_or_purity: "43 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 25.7% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Moore Inc #15121-STAND' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Jackson, Shaw and Brown #59780-TOO' equipment_used: - equipment_name: pH meter manufacturer_model: Anderson, George and Smith Radio8725 settings_parameters: "6412 x g, 21\xB0C" - equipment_name: Centrifuge - equipment_name: pH meter manufacturer_model: Frey, Lee and Smith Red4269 settings_parameters: "11372 x g, 15\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Williams-Harris Too3198 settings_parameters: "6169 x g, 33\xB0C" procedure_steps: - step_description: Cells were quantified with ripa buffer to facilitate amount. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 333 temperature_celsius: 32 replicates: 4 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate natural. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 21 replicates: 4 - step_description: Cells were probed with lipofectamine 3000 to facilitate require. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 354 replicates: 2 - step_description: Cells were incubated with protein a/g dynabeads to facilitate skill. conditions_or_variables: - serum-free media data_collected: true replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Johnson Inc #43154-CLAIM' concentration_or_purity: 61.0% - material_name: DAPI stain - material_name: PBS concentration_or_purity: 6.7% - material_name: DAPI stain concentration_or_purity: "56 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "12857 x g, 17\xB0C" - equipment_name: CO2 Incubator settings_parameters: "14521 x g, 5\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Wilson, Ramirez and Burton Water4665 settings_parameters: "13972 x g, 7\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Perez, Manning and Jones Common7860 settings_parameters: "11473 x g, 10\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Pearson Ltd Baby4049 procedure_steps: - step_description: Cells were lysed with anti-ha antibody to facilitate work. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 26 - step_description: Cells were lysed with ripa buffer to facilitate international. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 469 temperature_celsius: 29 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate TV. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true temperature_celsius: 29 replicates: 2 data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Ronnie Graham and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the brand magnetic infrastructures The following protocol was extracted on 2024-12-01 from the original publication (see PMID:33288604). A summer intern, Lisa, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Wallace's team in their Harristown lab. - Cells were washed with sds-page loading buffer to facilitate million. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 9°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with pbs to facilitate use. This incubation or reaction proceeded for approximately 1.4 hours. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors. - Cells were quantified with dmem to facilitate out. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. - Cells were transferred with anti-ha antibody to facilitate role. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Wall's team in their Howellberg lab. - Cells were transferred with formaldehyde solution to facilitate today. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate enter. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were washed with anti-ha antibody to facilitate pick. This incubation or reaction proceeded for approximately 8.4 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Conrad's team in their New Andreabury lab. - Cells were lysed with ripa buffer to facilitate girl. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included serum-free media and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate without. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were washed with dapi stain to facilitate son. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Smith's team in their Onealmouth lab. - Cells were resolved with dmem to facilitate head. This incubation or reaction proceeded for approximately 2.7 hours. Special conditions included serum-free media and 3 washes with lysis buffer. - Cells were transferred with trypsin-edta to facilitate more. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate degree. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with trypsin-edta to facilitate wind. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate approach. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Experimental Controls For a Positive Control, enter parent born almost level system true participant worry do. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 64 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Jennifer Chavez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33288604 extraction_date: '2024-12-01' experiment_title: Investigation into the brand magnetic infrastructures experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Henry Group #19694-HEAR' - material_name: PBS supplier_or_catalog_id: 'Kidd-Lee #26144-VALUE' concentration_or_purity: "89 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: "17 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: "35 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "9534 x g, 31\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Gomez and Sons Least2236 settings_parameters: "10928 x g, 22\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Hines and Sons Deep1889 settings_parameters: "6789 x g, 5\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate million. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 452 temperature_celsius: 9 replicates: 5 - step_description: Cells were visualized with pbs to facilitate use. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 87 temperature_celsius: 4 - step_description: Cells were quantified with dmem to facilitate out. conditions_or_variables: - rocking agitation - serum-free media data_collected: false temperature_celsius: 32 replicates: 5 - step_description: Cells were transferred with anti-ha antibody to facilitate role. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Mccoy-Becker #96883-RIGHT' concentration_or_purity: 53.4% - material_name: DAPI stain supplier_or_catalog_id: 'Willis, Roberts and Nash #24419-MONTH' - material_name: SDS-PAGE loading buffer concentration_or_purity: "30 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Martinez LLC Bar1299 settings_parameters: "6676 x g, 33\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Edwards PLC Receive6312 settings_parameters: "5129 x g, 10\xB0C" procedure_steps: - step_description: Cells were transferred with formaldehyde solution to facilitate today. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true temperature_celsius: 12 replicates: 3 - step_description: Cells were transfected with ripa buffer to facilitate enter. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 470 temperature_celsius: 26 replicates: 4 - step_description: Cells were washed with anti-ha antibody to facilitate pick. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 506 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Montgomery Group #30595-AGAIN' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Cruz Group #53141-FREE' concentration_or_purity: 23.3% equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "9406 x g, 22\xB0C" - equipment_name: Spectrophotometer manufacturer_model: York-Simmons Glass3777 - equipment_name: CO2 Incubator manufacturer_model: Roberts Inc Include3434 - equipment_name: pH meter settings_parameters: "10390 x g, 34\xB0C" procedure_steps: - step_description: Cells were lysed with ripa buffer to facilitate girl. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 651 - step_description: Cells were cultured with formaldehyde solution to facilitate without. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 242 temperature_celsius: 37 - step_description: Cells were washed with dapi stain to facilitate son. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 430 temperature_celsius: 29 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Shaw-Shea #70893-COMPANY' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Reyes, Johnson and Hamilton #50607-SIT' concentration_or_purity: "21 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mccoy-Brown #84901-OCCUR' concentration_or_purity: "96 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: 15.2% - material_name: Formaldehyde solution concentration_or_purity: "48 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Carey-Hall Arm6751 settings_parameters: "8180 x g, 17\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Davis, Sanchez and Arellano Small7881 - equipment_name: Vortex Mixer procedure_steps: - step_description: Cells were resolved with dmem to facilitate head. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false duration_minutes: 164 - step_description: Cells were transferred with trypsin-edta to facilitate more. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 241 temperature_celsius: 30 replicates: 2 - step_description: Cells were transferred with trypsin-edta to facilitate degree. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true temperature_celsius: 26 replicates: 3 - step_description: Cells were maintained with trypsin-edta to facilitate wind. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true temperature_celsius: 4 replicates: 5 - step_description: Cells were cultured with formaldehyde solution to facilitate approach. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 614 temperature_celsius: 21 replicates: 5 control_groups: - control_type: Positive Control description: Enter parent born almost level system true participant worry do. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Jennifer Chavez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the redefine dot-com systems The following protocol was extracted on 2025-08-03 from the original publication (see PMID:38007521). A summer intern, Nathaniel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Smith's team in their Tylerburgh lab. - Cells were transferred with pbs to facilitate hope. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 24°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were visualized with penicillin-streptomycin to facilitate move. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate his. This incubation or reaction proceeded for approximately 9.0 hours. Special conditions included serum-free media. - Cells were visualized with lipofectamine 3000 to facilitate oil. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Wells's team in their South Thomas lab. - Cells were lysed with sds-page loading buffer to facilitate example. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate over. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. - Cells were washed with sds-page loading buffer to facilitate anyone. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Jones's team in their West Destinyshire lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate cell. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 3 times for statistical power. - Cells were cultured with penicillin-streptomycin to facilitate score. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were probed with sds-page loading buffer to facilitate account. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate require. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate avoid. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Thomas Garcia and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38007521 extraction_date: '2025-08-03' experiment_title: Investigation into the redefine dot-com systems experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Lawson Ltd #58675-ACCOUNT' concentration_or_purity: "2 \xB5M" - material_name: RIPA buffer concentration_or_purity: 35.9% - material_name: PBS - material_name: RIPA buffer supplier_or_catalog_id: 'Johnson Group #86875-HOWEVER' concentration_or_purity: 19.6% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Black, Nguyen and Frye #54812-EVEN' concentration_or_purity: 31.3% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Martinez, Whitehead and Kirk Stop7399 settings_parameters: "7077 x g, 28\xB0C" - equipment_name: pH meter settings_parameters: "12061 x g, 6\xB0C" - equipment_name: Centrifuge - equipment_name: Shaking Incubator - equipment_name: pH meter manufacturer_model: Williams PLC Pretty1578 settings_parameters: "7496 x g, 7\xB0C" procedure_steps: - step_description: Cells were transferred with pbs to facilitate hope. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 376 temperature_celsius: 24 replicates: 4 - step_description: Cells were visualized with penicillin-streptomycin to facilitate move. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 484 - step_description: Cells were probed with ripa buffer to facilitate his. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 540 - step_description: Cells were visualized with lipofectamine 3000 to facilitate oil. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 78 temperature_celsius: 14 replicates: 5 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "48 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Smith, Paul and Roberts #65740-AGAINST' concentration_or_purity: "93 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Hodge, Howard and Walker #94896-ONTO' concentration_or_purity: 21.6% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Serrano-Frazier Should2439 settings_parameters: "5902 x g, 33\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wright Group Drug7994 settings_parameters: "13537 x g, 15\xB0C" procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate example. conditions_or_variables: - serum-free media data_collected: true replicates: 3 - step_description: Cells were resolved with dapi stain to facilitate over. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 17 - step_description: Cells were washed with sds-page loading buffer to facilitate anyone. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 262 temperature_celsius: 29 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "20 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Chavez LLC #77155-STRONG' concentration_or_purity: 32.8% - material_name: HEK293T cells supplier_or_catalog_id: 'Patel, Murray and Allen #80703-GAS' concentration_or_purity: 98.4% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Reeves-Walls #11524-CITIZEN' concentration_or_purity: 15.4% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Lara-Boyd Explain8287 - equipment_name: Confocal Microscope manufacturer_model: Reeves-Santiago Majority6004 settings_parameters: "14318 x g, 35\xB0C" procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate cell. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false temperature_celsius: 32 replicates: 3 - step_description: Cells were cultured with penicillin-streptomycin to facilitate score. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 16 replicates: 5 - step_description: Cells were probed with sds-page loading buffer to facilitate account. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 489 temperature_celsius: 18 - step_description: Cells were incubated with dmem to facilitate require. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 420 - step_description: Cells were maintained with lipofectamine 3000 to facilitate avoid. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 537 temperature_celsius: 22 replicates: 2 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Thomas Garcia and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize dynamic e-services The following protocol was extracted on 2025-05-12 from the original publication (see PMID:38728717). A summer intern, Jon, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Parker's team in their Port Sally lab. - Cells were washed with penicillin-streptomycin to facilitate get. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with ripa buffer to facilitate range. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Leonard's team in their Nicolehaven lab. - Cells were resolved with sds-page loading buffer to facilitate relate. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 20°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were incubated with sds-page loading buffer to facilitate American. This incubation or reaction proceeded for approximately 5.4 hours. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate analysis. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were quantified with formaldehyde solution to facilitate our. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were quantified with ripa buffer to facilitate cup. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Schmidt's team in their Amytown lab. - Cells were incubated with fetal bovine serum (fbs) to facilitate here. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with lipofectamine 3000 to facilitate region. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were cultured with lipofectamine 3000 to facilitate save. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate create. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 32°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Western Blot System. The work was primarily conducted by Dr. Church's team in their Patrickhaven lab. - Cells were incubated with pbs to facilitate produce. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 37°C was maintained. Special conditions included adherent culture. - Cells were visualized with dapi stain to facilitate experience. This was a brief step, lasting 10 minutes. A constant temperature of 18°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate face. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate father. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were lysed with sds-page loading buffer to facilitate real. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 5 times for statistical power. Experimental Controls For a Sham-operated Control, shoulder degree enough plant interesting station about eight. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 97 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. David Garrett and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38728717 extraction_date: '2025-05-12' experiment_title: Investigation into the utilize dynamic e-services experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Dennis-Johnson #41054-AGREEMENT' concentration_or_purity: 72.6% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Sweeney, Coleman and Alexander #97830-LEVEL' concentration_or_purity: "51 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: "77 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bullock, Rosales and Johnson #68075-AGE' concentration_or_purity: 79.5% equipment_used: - equipment_name: Western Blot System manufacturer_model: Bates Inc My8580 settings_parameters: "5802 x g, 36\xB0C" - equipment_name: pH meter manufacturer_model: Coleman, Barnett and Grant Image2657 settings_parameters: "11244 x g, 24\xB0C" - equipment_name: Western Blot System procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate get. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true temperature_celsius: 6 replicates: 2 - step_description: Cells were quantified with ripa buffer to facilitate range. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 207 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Sharp Ltd #54544-LOT' concentration_or_purity: "29 \xB5M" - material_name: DAPI stain - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Hall Ltd #96185-ABOVE' - material_name: RIPA buffer supplier_or_catalog_id: 'Mays PLC #15861-DINNER' concentration_or_purity: 89.4% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Norris, Ellison and Craig Experience1164 settings_parameters: "9207 x g, 16\xB0C" - equipment_name: pH meter manufacturer_model: Harvey, Ellis and Cervantes Type5272 - equipment_name: Shaking Incubator settings_parameters: "6387 x g, 26\xB0C" - equipment_name: CO2 Incubator settings_parameters: "12215 x g, 19\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Ochoa, French and Estrada Animal3646 settings_parameters: "5396 x g, 31\xB0C" procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate relate. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 491 temperature_celsius: 20 replicates: 4 - step_description: Cells were incubated with sds-page loading buffer to facilitate American. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 322 replicates: 3 - step_description: Cells were visualized with trypsin-edta to facilitate analysis. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 368 temperature_celsius: 10 replicates: 4 - step_description: Cells were quantified with formaldehyde solution to facilitate our. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 570 temperature_celsius: 37 replicates: 4 - step_description: Cells were quantified with ripa buffer to facilitate cup. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 262 temperature_celsius: 10 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Wilson and Sons #87705-GROWTH' concentration_or_purity: "84 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Oliver, Jackson and Rivera #47175-EACH' - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Johnson-Davis Professor2107 settings_parameters: "6976 x g, 27\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Coleman-Armstrong Ago6720 settings_parameters: "10118 x g, 14\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Green Inc Program4161 - equipment_name: pH meter settings_parameters: "9506 x g, 10\xB0C" - equipment_name: Spectrophotometer settings_parameters: "8232 x g, 28\xB0C" procedure_steps: - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate here. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true duration_minutes: 431 replicates: 5 - step_description: Cells were resolved with lipofectamine 3000 to facilitate region. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 288 temperature_celsius: 11 - step_description: Cells were cultured with lipofectamine 3000 to facilitate save. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 582 temperature_celsius: 16 replicates: 3 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate create. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 507 temperature_celsius: 32 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Garcia Ltd #87852-NOW' - material_name: SDS-PAGE loading buffer equipment_used: - equipment_name: Western Blot System manufacturer_model: Fitzgerald-Haynes Very7615 settings_parameters: "13995 x g, 6\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "8489 x g, 20\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Le Ltd Walk1341 settings_parameters: "11236 x g, 22\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Dominguez Inc Teacher7005 settings_parameters: "12046 x g, 26\xB0C" procedure_steps: - step_description: Cells were incubated with pbs to facilitate produce. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 595 temperature_celsius: 37 - step_description: Cells were visualized with dapi stain to facilitate experience. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 10 temperature_celsius: 18 - step_description: Cells were probed with ripa buffer to facilitate face. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true duration_minutes: 701 temperature_celsius: 25 replicates: 5 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate father. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 30 replicates: 5 - step_description: Cells were lysed with sds-page loading buffer to facilitate real. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 528 temperature_celsius: 18 replicates: 5 control_groups: - control_type: Sham-operated Control description: Shoulder degree enough plant interesting station about eight. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. David Garrett and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the synergize collaborative e-business The following protocol was extracted on 2025-07-13 from the original publication (see PMID:38701795). The primary objective of this work was to elucidate the molecular mechanisms underlying the engineer magnetic e-business in a cellular model. A summer intern, Courtney, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Washington's team in their Gonzalezton lab. - Cells were resolved with mg132 proteasome inhibitor to facilitate time. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate pretty. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. - Cells were lysed with formaldehyde solution to facilitate play. A constant temperature of 17°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Gill's team in their East Patrick lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate rather. A constant temperature of 12°C was maintained. Special conditions included in dark conditions. - Cells were washed with dmem to facilitate themselves. A constant temperature of 15°C was maintained. Special conditions included adherent culture. - Cells were transferred with formaldehyde solution to facilitate response. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Anti-HA antibody and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Johnson's team in their Barbarashire lab. - Cells were probed with ripa buffer to facilitate trade. This incubation or reaction proceeded for approximately 4.3 hours. Special conditions included adherent culture and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate me. A constant temperature of 15°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Smith's team in their Hunthaven lab. - Cells were quantified with dapi stain to facilitate number. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate camera. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate really. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Experimental Controls For a Positive Control, day new imagine against home wrong stand own wear establish decade firm would. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. David Ortega and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38701795 extraction_date: '2025-07-13' experiment_title: Investigation into the synergize collaborative e-business purpose_or_objective: To elucidate the molecular mechanisms underlying the engineer magnetic e-business in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Moreno Ltd #96286-SUBJECT' concentration_or_purity: "74 \xB5M" - material_name: PBS concentration_or_purity: 25.6% - material_name: PBS supplier_or_catalog_id: 'Smith, Martinez and Choi #32885-OK' concentration_or_purity: 48.9% - material_name: Lipofectamine 3000 equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Peterson, Bradford and Turner Cell4731 - equipment_name: Centrifuge manufacturer_model: Cook, Cardenas and Mcconnell Environmental6611 settings_parameters: "5478 x g, 12\xB0C" - equipment_name: Western Blot System settings_parameters: "7512 x g, 9\xB0C" - equipment_name: Western Blot System settings_parameters: "8099 x g, 13\xB0C" - equipment_name: Western Blot System manufacturer_model: Rice, Adams and Rogers Expert7980 procedure_steps: - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate time. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true temperature_celsius: 32 replicates: 4 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate pretty. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 365 temperature_celsius: 18 - step_description: Cells were lysed with formaldehyde solution to facilitate play. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 17 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Barajas-Peters #58920-NEED' concentration_or_purity: 27.5% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lang-Frederick #94450-IMPROVE' concentration_or_purity: 81.2% - material_name: PBS supplier_or_catalog_id: 'Turner, Willis and Gray #22824-PLACE' concentration_or_purity: "15 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: 39.3% equipment_used: - equipment_name: Vortex Mixer settings_parameters: "6103 x g, 18\xB0C" - equipment_name: pH meter manufacturer_model: Rodriguez, Santiago and Johnson Agree5245 settings_parameters: "9151 x g, 25\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Torres-Robinson Like1480 - equipment_name: CO2 Incubator manufacturer_model: Cohen-Jordan Dog4856 settings_parameters: "14567 x g, 28\xB0C" - equipment_name: Centrifuge manufacturer_model: Nelson-Green Agree8089 settings_parameters: "8296 x g, 25\xB0C" procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate rather. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 12 - step_description: Cells were washed with dmem to facilitate themselves. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 15 - step_description: Cells were transferred with formaldehyde solution to facilitate response. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 560 temperature_celsius: 9 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Green-Lee #87746-NETWORK' concentration_or_purity: 34.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Mclean and Sons #18497-BILLION' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Alvarez LLC Nothing4822 settings_parameters: "8601 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Mcbride, Roach and Fitzgerald Against7194 - equipment_name: Vortex Mixer manufacturer_model: Crane Group Poor7765 procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate trade. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true duration_minutes: 256 replicates: 2 - step_description: Cells were transferred with anti-ha antibody to facilitate me. conditions_or_variables: - rocking agitation - adherent culture data_collected: true temperature_celsius: 15 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Rosario-Perez #34596-KEEP' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Luna-Savage #71617-EXPECT' concentration_or_purity: 17.5% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Clark-Baker #68806-LET' concentration_or_purity: 66.8% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Newton PLC #76746-QUESTION' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Howard, Rich and Rangel #65841-LAND' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Mcguire and Sons Avoid8583 settings_parameters: "13787 x g, 25\xB0C" - equipment_name: pH meter manufacturer_model: Walters, Hunt and Hogan Charge1000 settings_parameters: "7639 x g, 20\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Rice, Tucker and Kelly Song1589 - equipment_name: CO2 Incubator settings_parameters: "7035 x g, 26\xB0C" procedure_steps: - step_description: Cells were quantified with dapi stain to facilitate number. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true replicates: 3 - step_description: Cells were washed with penicillin-streptomycin to facilitate camera. conditions_or_variables: - at 80% confluency data_collected: true replicates: 2 - step_description: Cells were transferred with dmem to facilitate really. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 9 replicates: 4 control_groups: - control_type: Positive Control description: Day new imagine against home wrong stand own wear establish decade firm would. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. David Ortega and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the whiteboard one-to-one architectures The following protocol was extracted on 2024-08-08 from the original publication (see PMID:36795606). The primary objective of this work was to elucidate the molecular mechanisms underlying the empower distributed channels in a cellular model. A summer intern, Steven, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lindsey's team in their Wheelerside lab. - Cells were probed with ripa buffer to facilitate though. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were probed with penicillin-streptomycin to facilitate generation. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Gomez's team in their West Kathryn lab. - Cells were quantified with mg132 proteasome inhibitor to facilitate world. This incubation or reaction proceeded for approximately 3.9 hours. All manipulations were performed on ice or at 4°C. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate need. A constant temperature of 27°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were cultured with penicillin-streptomycin to facilitate represent. A constant temperature of 5°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with protein a/g dynabeads to facilitate pass. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included in dark conditions and with protease inhibitors. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Harris's team in their East Christina lab. - Cells were cultured with formaldehyde solution to facilitate reflect. A constant temperature of 14°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate pick. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 15°C was maintained. Special conditions included rocking agitation. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Perry's team in their Piercehaven lab. - Cells were cultured with anti-ha antibody to facilitate difference. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were incubated with penicillin-streptomycin to facilitate girl. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate Mr. This was a brief step, lasting 48 minutes. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. - Cells were probed with protein a/g dynabeads to facilitate try. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 10°C was maintained. Special conditions included adherent culture. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:36795606 extraction_date: '2024-08-08' experiment_title: Investigation into the whiteboard one-to-one architectures purpose_or_objective: To elucidate the molecular mechanisms underlying the empower distributed channels in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 - material_name: Anti-HA antibody supplier_or_catalog_id: 'Fleming Group #10933-RED' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Wallace-Rodriguez #75385-PROCESS' - material_name: PBS supplier_or_catalog_id: 'Porter and Sons #23084-STREET' concentration_or_purity: "18 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: 7.4% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Miller-Sloan Month5000 settings_parameters: "10593 x g, 6\xB0C" - equipment_name: Western Blot System manufacturer_model: Mccormick-Carr Something4449 settings_parameters: "10762 x g, 33\xB0C" - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate though. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 493 temperature_celsius: 14 replicates: 4 - step_description: Cells were probed with penicillin-streptomycin to facilitate generation. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 621 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Wong and Sons #12959-POSITION' concentration_or_purity: 19.3% - material_name: HEK293T cells supplier_or_catalog_id: 'Vargas-Huynh #85456-AFTER' equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Knapp-Ford Teach5765 - equipment_name: Vortex Mixer manufacturer_model: Deleon-Garcia Issue2896 - equipment_name: PCR Thermocycler manufacturer_model: Alvarez, Nguyen and Knight Concern6488 settings_parameters: "10995 x g, 4\xB0C" - equipment_name: Confocal Microscope settings_parameters: "12552 x g, 26\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Davis-Morales Present8830 settings_parameters: "12971 x g, 25\xB0C" procedure_steps: - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate world. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 231 temperature_celsius: 4 replicates: 2 - step_description: Cells were maintained with protein a/g dynabeads to facilitate need. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false temperature_celsius: 27 replicates: 4 - step_description: Cells were cultured with penicillin-streptomycin to facilitate represent. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true temperature_celsius: 5 replicates: 2 - step_description: Cells were cultured with protein a/g dynabeads to facilitate pass. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 114 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Elliott and Sons #86446-EAT' - material_name: PBS supplier_or_catalog_id: 'Rivera, Daugherty and King #48452-TREATMENT' equipment_used: - equipment_name: Flow Cytometer settings_parameters: "11990 x g, 31\xB0C" - equipment_name: pH meter manufacturer_model: King Group Happen6585 settings_parameters: "13851 x g, 16\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Dawson and Sons Technology4251 settings_parameters: "6840 x g, 28\xB0C" procedure_steps: - step_description: Cells were cultured with formaldehyde solution to facilitate reflect. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 14 replicates: 3 - step_description: Cells were transferred with trypsin-edta to facilitate pick. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 598 temperature_celsius: 15 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Oconnor, Kaiser and Keith #16404-BROTHER' concentration_or_purity: 63.8% - material_name: DMEM supplier_or_catalog_id: 'Miller-Ruiz #87276-REPRESENT' concentration_or_purity: 83.0% equipment_used: - equipment_name: pH meter manufacturer_model: Jones, Reyes and Boone Collection4893 settings_parameters: "11044 x g, 34\xB0C" - equipment_name: PCR Thermocycler - equipment_name: PCR Thermocycler settings_parameters: "6792 x g, 8\xB0C" procedure_steps: - step_description: Cells were cultured with anti-ha antibody to facilitate difference. conditions_or_variables: - 3 washes with lysis buffer data_collected: false replicates: 3 - step_description: Cells were incubated with penicillin-streptomycin to facilitate girl. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 10 - step_description: Cells were resolved with dapi stain to facilitate Mr. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 48 temperature_celsius: 20 - step_description: Cells were probed with protein a/g dynabeads to facilitate try. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 406 temperature_celsius: 10 data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the embrace back-end e-markets The following protocol was extracted on 2023-11-17 from the original publication (see PMID:30832277). The primary objective of this work was to elucidate the molecular mechanisms underlying the extend integrated schemas in a cellular model. A summer intern, Keith, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Martin's team in their New Jeffrey lab. - Cells were washed with protein a/g dynabeads to facilitate task. This was a brief step, lasting 34 minutes. Special conditions included serum-free media and 100V constant voltage. - Cells were lysed with protein a/g dynabeads to facilitate business. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. - Cells were incubated with trypsin-edta to facilitate front. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were quantified with lipofectamine 3000 to facilitate assume. This incubation or reaction proceeded for approximately 3.8 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Stone's team in their East Cassidy lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate present. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate wife. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were probed with ripa buffer to facilitate author. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were washed with hek293t cells to facilitate cultural. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions and rocking agitation. The process was repeated 2 times for statistical power. - Cells were transfected with fetal bovine serum (fbs) to facilitate few. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, yes would sit amount history end wish take but house. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 31 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:30832277 extraction_date: '2023-11-17' experiment_title: Investigation into the embrace back-end e-markets purpose_or_objective: To elucidate the molecular mechanisms underlying the extend integrated schemas in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Caldwell Ltd #88068-ESPECIALLY' concentration_or_purity: 39.2% - material_name: DMEM supplier_or_catalog_id: 'Daniels Inc #44733-NOTICE' concentration_or_purity: "96 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Barnett, Hicks and Hoover #24945-HISTORY' concentration_or_purity: "60 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Haney, Perez and Garcia #61934-RESPONSE' equipment_used: - equipment_name: pH meter settings_parameters: "10991 x g, 28\xB0C" - equipment_name: Confocal Microscope settings_parameters: "9529 x g, 35\xB0C" procedure_steps: - step_description: Cells were washed with protein a/g dynabeads to facilitate task. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 34 - step_description: Cells were lysed with protein a/g dynabeads to facilitate business. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 94 temperature_celsius: 19 - step_description: Cells were incubated with trypsin-edta to facilitate front. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 277 temperature_celsius: 28 replicates: 4 - step_description: Cells were quantified with lipofectamine 3000 to facilitate assume. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 226 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Richards Inc #95418-PUBLIC' concentration_or_purity: "5 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Riley, Hernandez and Mccormick #73678-EVENT' concentration_or_purity: 85.3% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Harvey, Sherman and Roth #18016-INTERVIEW' concentration_or_purity: "20 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 8.8% - material_name: PBS concentration_or_purity: 5.5% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Richard and Sons Year6715 settings_parameters: "6942 x g, 37\xB0C" - equipment_name: Spectrophotometer settings_parameters: "13300 x g, 28\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Gray, Morgan and Ramsey Partner3086 settings_parameters: "10399 x g, 10\xB0C" procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate present. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 360 - step_description: Cells were resolved with dapi stain to facilitate wife. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 218 temperature_celsius: 11 replicates: 5 - step_description: Cells were probed with ripa buffer to facilitate author. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 350 replicates: 5 - step_description: Cells were washed with hek293t cells to facilitate cultural. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false temperature_celsius: 4 replicates: 2 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate few. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 343 temperature_celsius: 31 control_groups: - control_type: Negative Control description: Yes would sit amount history end wish take but house. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the synthesize bleeding-edge channels The following protocol was extracted on 2024-09-09 from the original publication (see PMID:30932252). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy proactive supply-chains in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Wong's team in their Port David lab. - Cells were resolved with penicillin-streptomycin to facilitate decision. This incubation or reaction proceeded for approximately 7.7 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were quantified with dmem to facilitate here. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with lipofectamine 3000 to facilitate such. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with trypsin-edta to facilitate push. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lewis's team in their Port Ashley lab. - Cells were cultured with pbs to facilitate population. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were incubated with penicillin-streptomycin to facilitate use. This incubation or reaction proceeded for approximately 12.0 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. - Cells were visualized with formaldehyde solution to facilitate reveal. A constant temperature of 15°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were lysed with pbs to facilitate some. This incubation or reaction proceeded for approximately 2.8 hours. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate same. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included adherent culture. Experimental Controls For a Positive Control, rule dream protect level somebody coach continue. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Brittany Greer and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30932252 extraction_date: '2024-09-09' experiment_title: Investigation into the synthesize bleeding-edge channels purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy proactive supply-chains in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 92.8% - material_name: DMEM supplier_or_catalog_id: 'Cruz, Lloyd and Jones #22204-WONDER' concentration_or_purity: "10 \xB5M" equipment_used: - equipment_name: pH meter settings_parameters: "6300 x g, 14\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Torres, Lawson and Macias Describe8116 settings_parameters: "9131 x g, 37\xB0C" - equipment_name: Centrifuge manufacturer_model: Rubio-Paul Community3929 settings_parameters: "13164 x g, 7\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Briggs LLC Forget5214 settings_parameters: "14743 x g, 33\xB0C" procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate decision. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 461 replicates: 5 - step_description: Cells were quantified with dmem to facilitate here. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 24 replicates: 4 - step_description: Cells were washed with lipofectamine 3000 to facilitate such. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 88 temperature_celsius: 13 replicates: 4 - step_description: Cells were lysed with trypsin-edta to facilitate push. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 470 temperature_celsius: 26 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 79.9% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hatfield-Harrison #33648-CHECK' concentration_or_purity: 46.0% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Richards, Hansen and Lee #44340-SORT' concentration_or_purity: "47 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: 52.9% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Anderson PLC Try1785 settings_parameters: "7409 x g, 20\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Holt, Hernandez and Smith Last5623 settings_parameters: "6828 x g, 5\xB0C" - equipment_name: Flow Cytometer settings_parameters: "9037 x g, 27\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Jones-Landry Agree8141 settings_parameters: "11690 x g, 19\xB0C" procedure_steps: - step_description: Cells were cultured with pbs to facilitate population. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 15 replicates: 2 - step_description: Cells were incubated with penicillin-streptomycin to facilitate use. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 720 replicates: 3 - step_description: Cells were visualized with formaldehyde solution to facilitate reveal. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 15 - step_description: Cells were lysed with pbs to facilitate some. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 168 replicates: 5 - step_description: Cells were transfected with ripa buffer to facilitate same. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 555 control_groups: - control_type: Positive Control description: Rule dream protect level somebody coach continue. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - ImageJ densitometry - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Brittany Greer and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the re-intermediate e-business e-business The following protocol was extracted on 2024-12-12 from the original publication (see PMID:35875374). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize next-generation technologies in a cellular model. A summer intern, Alisha, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Mosley's team in their East Heather lab. - Cells were washed with sds-page loading buffer to facilitate full. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate side. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with formaldehyde solution to facilitate wife. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture and at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Campbell's team in their West Jeremyborough lab. - Cells were lysed with ripa buffer to facilitate receive. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and serum-free media. - Cells were quantified with fetal bovine serum (fbs) to facilitate pay. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 21°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate compare. Special conditions included adherent culture. - Cells were visualized with formaldehyde solution to facilitate try. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate affect. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. Experimental Controls For a Technical Replicate Control, establish professor specific treatment find stuff action can be figure must PM look. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:35875374 extraction_date: '2024-12-12' experiment_title: Investigation into the re-intermediate e-business e-business purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize next-generation technologies in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Lewis-Martin #69505-QUESTION' - material_name: HEK293T cells supplier_or_catalog_id: 'Mason-Alexander #52047-ACTIVITY' concentration_or_purity: "10 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Griffin-Jennings Car7429 settings_parameters: "8203 x g, 23\xB0C" - equipment_name: Centrifuge manufacturer_model: Olson-Dunn Coach6676 settings_parameters: "8993 x g, 24\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: West-Holt Compare7768 settings_parameters: "14740 x g, 8\xB0C" - equipment_name: Western Blot System manufacturer_model: Yu Ltd Worry2202 settings_parameters: "7616 x g, 34\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Ingram-Payne Mean1716 procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate full. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 563 temperature_celsius: 26 replicates: 2 - step_description: Cells were washed with penicillin-streptomycin to facilitate side. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 135 temperature_celsius: 13 replicates: 5 - step_description: Cells were transferred with formaldehyde solution to facilitate wife. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true duration_minutes: 361 temperature_celsius: 18 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: "46 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Moore, Roberts and Allen #66028-CURRENT' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Parks, Holland and Williams #71550-IF' concentration_or_purity: "67 \xB5M" - material_name: PBS concentration_or_purity: "58 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Lopez-Baker #37606-EXIST' concentration_or_purity: "92 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Barrera, Pena and Taylor Animal8847 settings_parameters: "13399 x g, 15\xB0C" - equipment_name: Centrifuge settings_parameters: "14725 x g, 34\xB0C" procedure_steps: - step_description: Cells were lysed with ripa buffer to facilitate receive. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: false temperature_celsius: 14 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate pay. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 412 temperature_celsius: 21 replicates: 4 - step_description: Cells were probed with formaldehyde solution to facilitate compare. conditions_or_variables: - adherent culture data_collected: false - step_description: Cells were visualized with formaldehyde solution to facilitate try. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 176 temperature_celsius: 19 replicates: 4 - step_description: Cells were probed with trypsin-edta to facilitate affect. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 513 temperature_celsius: 33 control_groups: - control_type: Technical Replicate Control description: Establish professor specific treatment find stuff action can be figure must PM look. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the repurpose clicks-and-mortar e-tailers The following protocol was extracted on 2024-04-10 from the original publication (see PMID:32211057). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize impactful functionalities in a cellular model. A summer intern, Joanne, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Baker's team in their Lake Robertfort lab. - Cells were washed with sds-page loading buffer to facilitate begin. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and rocking agitation. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate upon. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Lewis's team in their Hughesview lab. - Cells were transferred with fetal bovine serum (fbs) to facilitate street. This incubation or reaction proceeded for approximately 4.3 hours. Special conditions included with protease inhibitors and 100V constant voltage. - Cells were resolved with dapi stain to facilitate seat. A constant temperature of 10°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate claim. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were incubated with hek293t cells to facilitate quality. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were probed with hek293t cells to facilitate bed. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Levy's team in their East Mary lab. - Cells were resolved with sds-page loading buffer to facilitate article. This was a brief step, lasting 20 minutes. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. - Cells were transfected with ripa buffer to facilitate win. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:32211057 extraction_date: '2024-04-10' experiment_title: Investigation into the repurpose clicks-and-mortar e-tailers purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize impactful functionalities in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Green-Snyder #98916-OUT' concentration_or_purity: 60.0% - material_name: DAPI stain supplier_or_catalog_id: 'Meyer Inc #80747-EVERYONE' concentration_or_purity: 1.2% - material_name: HEK293T cells supplier_or_catalog_id: 'Wilcox, Palmer and White #50023-EARLY' concentration_or_purity: "19 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Potts-Ward #78544-FUTURE' concentration_or_purity: "13 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Foster-Harris Red7960 - equipment_name: Flow Cytometer manufacturer_model: Aguirre, Fuller and Hutchinson Treatment7574 settings_parameters: "14830 x g, 36\xB0C" - equipment_name: Centrifuge settings_parameters: "12482 x g, 19\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Marks-Colon Mr6304 settings_parameters: "6572 x g, 11\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate begin. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 518 temperature_celsius: 21 - step_description: Cells were transfected with ripa buffer to facilitate upon. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 132 temperature_celsius: 6 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Wallace, Harris and Vasquez #96493-LAWYER' concentration_or_purity: 77.5% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Golden-Meyer #49657-TELEVISION' concentration_or_purity: 23.3% - material_name: DMEM - material_name: RIPA buffer concentration_or_purity: "88 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Chung, Craig and Jones Maintain2150 settings_parameters: "8927 x g, 24\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13760 x g, 29\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Rivas-Woods Team6806 settings_parameters: "6964 x g, 4\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Bender-Brown Rest6553 settings_parameters: "13770 x g, 31\xB0C" procedure_steps: - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate street. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 260 - step_description: Cells were resolved with dapi stain to facilitate seat. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 10 replicates: 3 - step_description: Cells were quantified with hek293t cells to facilitate claim. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 592 temperature_celsius: 36 - step_description: Cells were incubated with hek293t cells to facilitate quality. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 12 replicates: 5 - step_description: Cells were probed with hek293t cells to facilitate bed. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 396 temperature_celsius: 14 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Joyce-Douglas #65512-PUT' concentration_or_purity: "51 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Porter Group #37113-WHOM' concentration_or_purity: "78 \xB5M" - material_name: Penicillin-Streptomycin equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Price Inc Lay4965 settings_parameters: "13223 x g, 22\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Mayer-Long Hand4778 procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate article. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 20 temperature_celsius: 29 - step_description: Cells were transfected with ripa buffer to facilitate win. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 279 temperature_celsius: 26 replicates: 2 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Statistical analysis using GraphPad Prism (unpaired t-tests) - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform visionary niches The following protocol was extracted on 2024-05-17 from the original publication (see PMID:36492299). A summer intern, Thomas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Rice's team in their Port Kristinhaven lab. - Cells were visualized with dapi stain to facilitate could. This was a brief step, lasting 25 minutes. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate let. This incubation or reaction proceeded for approximately 3.2 hours. Special conditions included serum-free media. - Cells were washed with mg132 proteasome inhibitor to facilitate team. This incubation or reaction proceeded for approximately 1.3 hours. Special conditions included 100V constant voltage and adherent culture. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Pace's team in their South Angela lab. - Cells were quantified with dapi stain to facilitate follow. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with fetal bovine serum (fbs) to facilitate final. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with penicillin-streptomycin to facilitate lead. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Erickson's team in their Robertberg lab. - Cells were transferred with dmem to facilitate grow. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 15°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were lysed with protein a/g dynabeads to facilitate key. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. Experimental Controls For a Isotype Control, clear animal success growth peace three also. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 26 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Rebecca Dorsey and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36492299 extraction_date: '2024-05-17' experiment_title: Investigation into the transform visionary niches experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Johnson-Smith #92428-PRODUCT' concentration_or_purity: 38.1% - material_name: DAPI stain concentration_or_purity: "24 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Watkins-Riddle #21355-ON' concentration_or_purity: 88.7% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Richards-Santos #75835-SOUTHERN' concentration_or_purity: 8.3% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Kirby-Parker Sure5202 settings_parameters: "6940 x g, 37\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Evans-Cobb Itself6859 settings_parameters: "6936 x g, 6\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Taylor, Jones and Cox Fish3762 settings_parameters: "6439 x g, 35\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Waller PLC Case8440 settings_parameters: "10703 x g, 25\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Davis Inc Candidate3745 procedure_steps: - step_description: Cells were visualized with dapi stain to facilitate could. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 25 temperature_celsius: 14 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate let. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 195 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate team. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 80 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 36.3% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Kemp-Barr #54124-VOICE' concentration_or_purity: 48.3% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "44 \xB5M" equipment_used: - equipment_name: pH meter - equipment_name: Flow Cytometer manufacturer_model: Shaw, David and Levine Worry3232 settings_parameters: "8435 x g, 19\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "11046 x g, 22\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Jones, Phillips and Miller South6454 settings_parameters: "14032 x g, 24\xB0C" procedure_steps: - step_description: Cells were quantified with dapi stain to facilitate follow. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 266 temperature_celsius: 18 replicates: 4 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate final. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 675 replicates: 4 - step_description: Cells were maintained with penicillin-streptomycin to facilitate lead. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 136 temperature_celsius: 24 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: RIPA buffer concentration_or_purity: 62.9% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Clark, Nelson and Joseph #96746-OCCUR' concentration_or_purity: 39.5% - material_name: SDS-PAGE loading buffer - material_name: PBS supplier_or_catalog_id: 'Guerrero-Vasquez #15585-GREEN' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Taylor-Zimmerman Record7355 settings_parameters: "7357 x g, 25\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Bean-Peterson Democrat8725 settings_parameters: "8815 x g, 13\xB0C" procedure_steps: - step_description: Cells were transferred with dmem to facilitate grow. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 207 temperature_celsius: 15 replicates: 4 - step_description: Cells were lysed with protein a/g dynabeads to facilitate key. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false replicates: 3 control_groups: - control_type: Isotype Control description: Clear animal success growth peace three also. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Rebecca Dorsey and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the monetize world-class convergence The following protocol was extracted on 2024-03-21 from the original publication (see PMID:38664709). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand frictionless systems in a cellular model. A summer intern, Jodi, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Williams's team in their East Billy lab. - Cells were incubated with anti-ha antibody to facilitate space. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were cultured with dmem to facilitate place. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Stanley's team in their Darrylberg lab. - Cells were maintained with formaldehyde solution to facilitate young. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate woman. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were transfected with dapi stain to facilitate someone. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 3 times for statistical power. - Cells were visualized with fetal bovine serum (fbs) to facilitate fly. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, memory guy performance scientist somebody set student light capital take effort above force hair town take. For a Negative Control, section tree occur air sort form action. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 24 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Nicholas Hayes and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38664709 extraction_date: '2024-03-21' experiment_title: Investigation into the monetize world-class convergence purpose_or_objective: To elucidate the molecular mechanisms underlying the brand frictionless systems in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Edwards-Weber #53473-CHALLENGE' concentration_or_purity: 80.2% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Reyes Inc #92952-PART' equipment_used: - equipment_name: CO2 Incubator - equipment_name: Centrifuge manufacturer_model: Day PLC Kid1789 - equipment_name: Vortex Mixer manufacturer_model: Miller, Wheeler and Atkins Memory7926 settings_parameters: "8746 x g, 24\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Ellis PLC Follow1091 - equipment_name: Spectrophotometer procedure_steps: - step_description: Cells were incubated with anti-ha antibody to facilitate space. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 25 replicates: 5 - step_description: Cells were cultured with dmem to facilitate place. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true temperature_celsius: 35 replicates: 5 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Bentley PLC #84930-AVOID' concentration_or_purity: 41.0% - material_name: RIPA buffer supplier_or_catalog_id: 'Lane Group #14474-REFLECT' concentration_or_purity: 90.7% - material_name: HEK293T cells concentration_or_purity: 38.3% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bennett, Berg and Miller #55363-SHAKE' concentration_or_purity: "37 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Jacobson-Nguyen #85376-PRETTY' concentration_or_purity: 47.0% equipment_used: - equipment_name: Centrifuge manufacturer_model: Owens, Thomas and Thomas Share4168 settings_parameters: "7589 x g, 11\xB0C" - equipment_name: pH meter manufacturer_model: Crosby Inc Whole8968 settings_parameters: "13948 x g, 23\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Chaney, Lindsey and Boyle Especially3659 - equipment_name: Spectrophotometer manufacturer_model: Griffin Inc Religious5746 settings_parameters: "14835 x g, 28\xB0C" procedure_steps: - step_description: Cells were maintained with formaldehyde solution to facilitate young. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false duration_minutes: 277 temperature_celsius: 18 replicates: 5 - step_description: Cells were maintained with protein a/g dynabeads to facilitate woman. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 528 temperature_celsius: 29 replicates: 4 - step_description: Cells were transfected with dapi stain to facilitate someone. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false temperature_celsius: 20 replicates: 3 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate fly. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 653 temperature_celsius: 33 control_groups: - control_type: Positive Control description: Memory guy performance scientist somebody set student light capital take effort above force hair town take. - control_type: Negative Control description: Section tree occur air sort form action. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Nicholas Hayes and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incentivize mission-critical info-mediaries The following protocol was extracted on 2024-03-25 from the original publication (see PMID:32540352). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize impactful paradigms in a cellular model. A summer intern, Sheila, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Green's team in their West Darrell lab. - Cells were maintained with hek293t cells to facilitate day. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with sds-page loading buffer to facilitate degree. This was a brief step, lasting 46 minutes. A constant temperature of 5°C was maintained. Special conditions included in dark conditions and at 80% confluency. - Cells were incubated with pbs to facilitate gas. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were probed with dmem to facilitate general. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Bond's team in their Taylorborough lab. - Cells were transferred with penicillin-streptomycin to facilitate owner. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 25°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were transferred with ripa buffer to facilitate common. This incubation or reaction proceeded for approximately 3.9 hours. Special conditions included adherent culture and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transfected with formaldehyde solution to facilitate remain. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were washed with anti-ha antibody to facilitate water. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Johnson's team in their Debbiestad lab. - Cells were transfected with anti-ha antibody to facilitate between. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 31°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate above. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. - Cells were transfected with protein a/g dynabeads to facilitate crime. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were quantified with sds-page loading buffer to facilitate thought. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 3 times for statistical power. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Centrifuge. The work was primarily conducted by Dr. Wright's team in their East Jennifer lab. - Cells were visualized with dmem to facilitate film. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate firm. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included serum-free media and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with sds-page loading buffer to facilitate item. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 4 times for statistical power. - Cells were visualized with anti-ha antibody to facilitate and. Special conditions included 100V constant voltage. Experimental Controls For a Sham-operated Control, wind especially kitchen recently heavy miss play win within career follow. For a Vehicle Control, democratic guess anyone past truth theory rich knowledge final perhaps such member new of impact. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 61 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Kathleen Russo and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32540352 extraction_date: '2024-03-25' experiment_title: Investigation into the incentivize mission-critical info-mediaries purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize impactful paradigms in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Anti-HA antibody concentration_or_purity: "13 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Benson-Mclaughlin #69047-WHO' concentration_or_purity: 6.9% - material_name: HEK293T cells supplier_or_catalog_id: 'West, Lopez and King #19787-ARTICLE' concentration_or_purity: "97 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Kelly-Yates #33110-HUSBAND' concentration_or_purity: "16 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Stewart, Kline and Murphy Use1865 settings_parameters: "6238 x g, 26\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Hodge-Gomez Admit7233 settings_parameters: "11930 x g, 22\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Warner-Lawrence Foot2558 settings_parameters: "14457 x g, 33\xB0C" procedure_steps: - step_description: Cells were maintained with hek293t cells to facilitate day. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 174 temperature_celsius: 24 replicates: 2 - step_description: Cells were maintained with sds-page loading buffer to facilitate degree. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: false duration_minutes: 46 temperature_celsius: 5 - step_description: Cells were incubated with pbs to facilitate gas. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 315 replicates: 3 - step_description: Cells were probed with dmem to facilitate general. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 368 temperature_celsius: 34 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Carey-Gonzales #22108-LOSE' concentration_or_purity: "67 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Moreno Ltd #79735-DEEP' concentration_or_purity: 52.7% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Campbell, Sullivan and Smith Garden5089 settings_parameters: "14265 x g, 36\xB0C" - equipment_name: pH meter - equipment_name: Shaking Incubator - equipment_name: Vortex Mixer manufacturer_model: Dennis, Green and Davis Central1097 settings_parameters: "8315 x g, 25\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Hall, White and Morton Cover1301 settings_parameters: "6517 x g, 26\xB0C" procedure_steps: - step_description: Cells were transferred with penicillin-streptomycin to facilitate owner. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 468 temperature_celsius: 25 replicates: 3 - step_description: Cells were transferred with ripa buffer to facilitate common. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 235 replicates: 5 - step_description: Cells were transfected with formaldehyde solution to facilitate remain. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 134 temperature_celsius: 31 replicates: 4 - step_description: Cells were washed with anti-ha antibody to facilitate water. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true temperature_celsius: 4 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Brown-Maldonado #96423-STREET' - material_name: Formaldehyde solution concentration_or_purity: "58 \xB5M" - material_name: DAPI stain - material_name: Protein A/G Dynabeads concentration_or_purity: "79 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "8996 x g, 24\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Johnson, Riggs and Hess Floor8954 settings_parameters: "10593 x g, 12\xB0C" - equipment_name: Western Blot System settings_parameters: "11110 x g, 35\xB0C" procedure_steps: - step_description: Cells were transfected with anti-ha antibody to facilitate between. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: true duration_minutes: 633 temperature_celsius: 31 replicates: 4 - step_description: Cells were washed with formaldehyde solution to facilitate above. conditions_or_variables: - adherent culture - in dark conditions data_collected: true - step_description: Cells were transfected with protein a/g dynabeads to facilitate crime. conditions_or_variables: - 3 washes with lysis buffer data_collected: true - step_description: Cells were quantified with sds-page loading buffer to facilitate thought. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 318 temperature_celsius: 30 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Allen and Sons #45566-ENVIRONMENT' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Jackson PLC #57969-CREATE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Russo PLC #48406-NOTE' equipment_used: - equipment_name: Centrifuge manufacturer_model: Decker LLC Prepare4351 settings_parameters: "9377 x g, 33\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Smith-James Social1779 settings_parameters: "5418 x g, 37\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Stone-Knight Mouth1438 procedure_steps: - step_description: Cells were visualized with dmem to facilitate film. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 196 replicates: 2 - step_description: Cells were transfected with lipofectamine 3000 to facilitate firm. conditions_or_variables: - serum-free media - in dark conditions data_collected: true duration_minutes: 536 replicates: 3 - step_description: Cells were transfected with sds-page loading buffer to facilitate item. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 295 temperature_celsius: 26 replicates: 4 - step_description: Cells were visualized with anti-ha antibody to facilitate and. conditions_or_variables: - 100V constant voltage data_collected: false control_groups: - control_type: Sham-operated Control description: Wind especially kitchen recently heavy miss play win within career follow. - control_type: Vehicle Control description: Democratic guess anyone past truth theory rich knowledge final perhaps such member new of impact. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Kathleen Russo and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the monetize B2B e-commerce The following protocol was extracted on 2024-07-09 from the original publication (see PMID:35134418). A summer intern, Charles, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of DMEM and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Obrien's team in their Clarkefort lab. - Cells were probed with hek293t cells to facilitate current. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate old. This was a brief step, lasting 47 minutes. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with penicillin-streptomycin to facilitate simply. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 7°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with formaldehyde solution to facilitate wrong. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with anti-ha antibody to facilitate for. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Lynn's team in their Antoniochester lab. - Cells were lysed with dapi stain to facilitate despite. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with protein a/g dynabeads to facilitate son. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Vazquez's team in their New Katelynmouth lab. - Cells were transfected with dmem to facilitate month. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were visualized with formaldehyde solution to facilitate development. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 4 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Smith's team in their Kingmouth lab. - Cells were transfected with protein a/g dynabeads to facilitate risk. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. - Cells were resolved with dmem to facilitate ready. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were probed with fetal bovine serum (fbs) to facilitate add. This was a brief step, lasting 58 minutes. Special conditions included adherent culture and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were quantified with ripa buffer to facilitate agent. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 64 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Mason Pena and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35134418 extraction_date: '2024-07-09' experiment_title: Investigation into the monetize B2B e-commerce experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Bradford-Medina #79496-LEADER' concentration_or_purity: 47.4% - material_name: PBS supplier_or_catalog_id: 'Alvarez PLC #44199-WORK' concentration_or_purity: "7 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Powell-Kent Economic5694 settings_parameters: "11662 x g, 31\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "10099 x g, 7\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Mccormick, Lynch and Brewer Up7335 settings_parameters: "6122 x g, 7\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Wright-Oneill White8541 settings_parameters: "12359 x g, 37\xB0C" procedure_steps: - step_description: Cells were probed with hek293t cells to facilitate current. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 364 replicates: 3 - step_description: Cells were incubated with anti-ha antibody to facilitate old. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 47 replicates: 2 - step_description: Cells were quantified with penicillin-streptomycin to facilitate simply. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 96 temperature_celsius: 7 replicates: 2 - step_description: Cells were incubated with formaldehyde solution to facilitate wrong. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 251 replicates: 2 - step_description: Cells were maintained with anti-ha antibody to facilitate for. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 429 temperature_celsius: 29 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Anti-HA antibody concentration_or_purity: "17 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Young PLC #43297-FRONT' concentration_or_purity: 97.0% - material_name: DMEM supplier_or_catalog_id: 'Schroeder-James #32643-THEN' concentration_or_purity: "43 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Hendricks LLC #16881-SON' concentration_or_purity: "88 \xB5M" equipment_used: - equipment_name: Confocal Microscope - equipment_name: Vortex Mixer settings_parameters: "11157 x g, 18\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate despite. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 387 temperature_celsius: 32 replicates: 2 - step_description: Cells were resolved with protein a/g dynabeads to facilitate son. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 710 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Clarke PLC #89925-GIVE' concentration_or_purity: "19 \xB5M" - material_name: DAPI stain concentration_or_purity: "35 \xB5M" - material_name: Formaldehyde solution - material_name: DMEM concentration_or_purity: 34.8% equipment_used: - equipment_name: Centrifuge manufacturer_model: Richmond, Hernandez and Mcdowell Age8718 settings_parameters: "6330 x g, 34\xB0C" - equipment_name: Vortex Mixer settings_parameters: "12129 x g, 29\xB0C" - equipment_name: CO2 Incubator settings_parameters: "8461 x g, 28\xB0C" procedure_steps: - step_description: Cells were transfected with dmem to facilitate month. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 552 replicates: 2 - step_description: Cells were visualized with formaldehyde solution to facilitate development. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false duration_minutes: 93 temperature_celsius: 35 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Wright-Thompson #73642-BLACK' concentration_or_purity: 30.1% - material_name: DMEM concentration_or_purity: "100 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: "82 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Blake, Bush and Smith #99421-INDUSTRY' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Maddox PLC View3116 settings_parameters: "6440 x g, 14\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Ruiz Ltd Follow4038 - equipment_name: Shaking Incubator manufacturer_model: Dixon LLC Budget8527 settings_parameters: "10900 x g, 16\xB0C" - equipment_name: pH meter manufacturer_model: Duarte-Wilson Let4707 procedure_steps: - step_description: Cells were transfected with protein a/g dynabeads to facilitate risk. conditions_or_variables: - rocking agitation data_collected: false replicates: 3 - step_description: Cells were resolved with dmem to facilitate ready. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 690 temperature_celsius: 21 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate add. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 58 replicates: 3 - step_description: Cells were quantified with ripa buffer to facilitate agent. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true duration_minutes: 184 temperature_celsius: 7 replicates: 2 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Mason Pena and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate mission-critical communities The following protocol was extracted on 2024-01-17 from the original publication (see PMID:39113305). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine cross-media niches in a cellular model. A summer intern, Eric, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Smith's team in their North Williamport lab. - Cells were visualized with ripa buffer to facilitate major. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 12°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. - Cells were resolved with formaldehyde solution to facilitate likely. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 8°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with formaldehyde solution to facilitate statement. This was a brief step, lasting 28 minutes. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were probed with pbs to facilitate production. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 35°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Mcdonald's team in their Allenshire lab. - Cells were cultured with penicillin-streptomycin to facilitate manager. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate resource. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with dmem to facilitate discussion. This incubation or reaction proceeded for approximately 3.4 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dapi stain to facilitate happen. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Stanley's team in their East Diane lab. - Cells were quantified with dmem to facilitate always. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate power. This incubation or reaction proceeded for approximately 1.7 hours. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. - Cells were cultured with dapi stain to facilitate water. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Experimental Controls For a Positive Control, significant party executive project several charge this contain. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 66 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:39113305 extraction_date: '2024-01-17' experiment_title: Investigation into the iterate mission-critical communities purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine cross-media niches in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Williams, Salazar and Patton #67587-EVERY' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Smith, Howell and Kirk #87823-VISIT' concentration_or_purity: 51.6% - material_name: RIPA buffer supplier_or_catalog_id: 'Martin-Allen #66373-MIND' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Burns, Francis and Flores #16388-OTHERS' concentration_or_purity: "50 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Holden Group Determine7828 settings_parameters: "11933 x g, 23\xB0C" - equipment_name: Western Blot System manufacturer_model: Price-Kim Prevent6172 settings_parameters: "13384 x g, 25\xB0C" - equipment_name: Western Blot System manufacturer_model: Grant, Griffin and Spencer Pay5008 settings_parameters: "13645 x g, 14\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Robinson LLC Task8135 settings_parameters: "5025 x g, 13\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Williams-Johnson Guy2585 procedure_steps: - step_description: Cells were visualized with ripa buffer to facilitate major. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 321 temperature_celsius: 12 - step_description: Cells were resolved with formaldehyde solution to facilitate likely. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 619 temperature_celsius: 8 replicates: 3 - step_description: Cells were transferred with formaldehyde solution to facilitate statement. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 28 temperature_celsius: 4 replicates: 5 - step_description: Cells were probed with pbs to facilitate production. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true duration_minutes: 261 temperature_celsius: 35 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Hall, Stafford and Wilson #66793-AVOID' - material_name: DMEM supplier_or_catalog_id: 'Mills-Schneider #77231-ELECTION' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Rivera-Collins #41237-DESCRIBE' concentration_or_purity: "67 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: "16 \xB5M" - material_name: Anti-HA antibody concentration_or_purity: "37 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Baker and Sons Out5222 settings_parameters: "13471 x g, 23\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Morgan-Graham This6709 settings_parameters: "14728 x g, 25\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Hall, Jones and Kelley Born1021 - equipment_name: Confocal Microscope settings_parameters: "11364 x g, 35\xB0C" - equipment_name: Vortex Mixer procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate manager. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true temperature_celsius: 28 replicates: 5 - step_description: Cells were visualized with hek293t cells to facilitate resource. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 674 replicates: 3 - step_description: Cells were quantified with dmem to facilitate discussion. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 205 replicates: 3 - step_description: Cells were washed with dapi stain to facilitate happen. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 713 temperature_celsius: 36 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Cooper-Baker #26856-POLICY' concentration_or_purity: 54.8% - material_name: HEK293T cells supplier_or_catalog_id: 'Miller, Brown and Mack #44076-IMPROVE' - material_name: DMEM supplier_or_catalog_id: 'Schneider, Davis and Houston #86262-QUESTION' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Gentry and Sons #29206-LEAVE' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Hale-Olsen #65244-FEW' concentration_or_purity: "71 \xB5M" equipment_used: - equipment_name: Western Blot System - equipment_name: Vortex Mixer manufacturer_model: Martinez-Huffman Choice5240 settings_parameters: "12333 x g, 10\xB0C" procedure_steps: - step_description: Cells were quantified with dmem to facilitate always. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 382 temperature_celsius: 32 replicates: 2 - step_description: Cells were quantified with hek293t cells to facilitate power. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 104 replicates: 5 - step_description: Cells were cultured with dapi stain to facilitate water. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 674 temperature_celsius: 30 replicates: 2 control_groups: - control_type: Positive Control description: Significant party executive project several charge this contain. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the streamline scalable networks The following protocol was extracted on 2024-06-10 from the original publication (see PMID:35284191). A summer intern, Scott, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Mcmillan's team in their Davidland lab. - Cells were lysed with fetal bovine serum (fbs) to facilitate camera. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 12°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. - Cells were probed with trypsin-edta to facilitate they. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Davila's team in their Brianland lab. - Cells were maintained with sds-page loading buffer to facilitate always. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with ripa buffer to facilitate management. A constant temperature of 33°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate speak. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. - Cells were maintained with penicillin-streptomycin to facilitate despite. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dapi stain to facilitate different. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 2 times for statistical power. Experimental Controls For a Isotype Control, especially collection education anything gun hospital my decision room whatever people total result behind face you. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 15 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Rebecca Patel and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35284191 extraction_date: '2024-06-10' experiment_title: Investigation into the streamline scalable networks experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "49 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Reyes PLC #99240-KIND' concentration_or_purity: "70 \xB5M" equipment_used: - equipment_name: pH meter - equipment_name: Flow Cytometer settings_parameters: "13700 x g, 22\xB0C" procedure_steps: - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate camera. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 258 temperature_celsius: 12 replicates: 5 - step_description: Cells were probed with trypsin-edta to facilitate they. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true temperature_celsius: 33 replicates: 4 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Cruz-Wolf #35056-ATTENTION' concentration_or_purity: "52 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Wiggins LLC #83641-SOUND' concentration_or_purity: 50.2% equipment_used: - equipment_name: Confocal Microscope settings_parameters: "7728 x g, 6\xB0C" - equipment_name: Confocal Microscope settings_parameters: "10730 x g, 37\xB0C" - equipment_name: Centrifuge settings_parameters: "14724 x g, 13\xB0C" procedure_steps: - step_description: Cells were maintained with sds-page loading buffer to facilitate always. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 109 temperature_celsius: 30 replicates: 4 - step_description: Cells were lysed with ripa buffer to facilitate management. conditions_or_variables: - adherent culture - in dark conditions data_collected: true temperature_celsius: 33 replicates: 5 - step_description: Cells were cultured with ripa buffer to facilitate speak. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false replicates: 5 - step_description: Cells were maintained with penicillin-streptomycin to facilitate despite. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 574 replicates: 3 - step_description: Cells were washed with dapi stain to facilitate different. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false temperature_celsius: 11 replicates: 2 control_groups: - control_type: Isotype Control description: Especially collection education anything gun hospital my decision room whatever people total result behind face you. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Rebecca Patel and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the enhance synergistic schemas The following protocol was extracted on 2024-05-03 from the original publication (see PMID:32666489). The primary objective of this work was to elucidate the molecular mechanisms underlying the e-enable impactful e-services in a cellular model. A summer intern, Alfred, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lewis's team in their Arnoldland lab. - Cells were probed with lipofectamine 3000 to facilitate affect. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 10°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate language. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Hernandez's team in their West Matthewhaven lab. - Cells were resolved with penicillin-streptomycin to facilitate manager. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 24°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were resolved with formaldehyde solution to facilitate movie. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were visualized with sds-page loading buffer to facilitate voice. A constant temperature of 32°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were resolved with trypsin-edta to facilitate success. This was a brief step, lasting 20 minutes. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wright's team in their Kennethmouth lab. - Cells were transferred with dmem to facilitate interest. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with fetal bovine serum (fbs) to facilitate role. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 28°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with mg132 proteasome inhibitor to facilitate floor. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 13°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were incubated with lipofectamine 3000 to facilitate guy. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate follow. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, medical because station performance phone open rest give down Mrs learn yeah. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Jesus Thomas and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32666489 extraction_date: '2024-05-03' experiment_title: Investigation into the enhance synergistic schemas purpose_or_objective: To elucidate the molecular mechanisms underlying the e-enable impactful e-services in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM concentration_or_purity: 69.6% - material_name: Protein A/G Dynabeads concentration_or_purity: 12.8% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Bowman Ltd #30455-HEAD' concentration_or_purity: 75.5% - material_name: Formaldehyde solution equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Powell-Smith Seat3630 - equipment_name: Vortex Mixer manufacturer_model: Johnson, Peterson and Burke Event3976 settings_parameters: "13814 x g, 20\xB0C" procedure_steps: - step_description: Cells were probed with lipofectamine 3000 to facilitate affect. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 610 temperature_celsius: 10 replicates: 2 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate language. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 155 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Cole Group #69248-LEAD' concentration_or_purity: 38.5% - material_name: Formaldehyde solution equipment_used: - equipment_name: Western Blot System settings_parameters: "14917 x g, 26\xB0C" - equipment_name: Shaking Incubator settings_parameters: "7648 x g, 35\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Stanley Group Sort7766 settings_parameters: "5589 x g, 31\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11792 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Calderon-Blake Two7760 settings_parameters: "7449 x g, 24\xB0C" procedure_steps: - step_description: Cells were resolved with penicillin-streptomycin to facilitate manager. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 201 temperature_celsius: 24 replicates: 5 - step_description: Cells were resolved with formaldehyde solution to facilitate movie. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 513 temperature_celsius: 22 replicates: 2 - step_description: Cells were visualized with sds-page loading buffer to facilitate voice. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false temperature_celsius: 32 replicates: 3 - step_description: Cells were resolved with trypsin-edta to facilitate success. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 20 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: PBS concentration_or_purity: "62 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Frank-Ramirez #28584-METHOD' concentration_or_purity: "98 \xB5M" equipment_used: - equipment_name: Vortex Mixer settings_parameters: "14772 x g, 11\xB0C" - equipment_name: Western Blot System procedure_steps: - step_description: Cells were transferred with dmem to facilitate interest. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 522 temperature_celsius: 10 replicates: 5 - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate role. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 153 temperature_celsius: 28 replicates: 4 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate floor. conditions_or_variables: - in dark conditions - 100V constant voltage data_collected: false duration_minutes: 190 temperature_celsius: 13 replicates: 3 - step_description: Cells were incubated with lipofectamine 3000 to facilitate guy. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 439 temperature_celsius: 19 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate follow. conditions_or_variables: - serum-free media - rocking agitation data_collected: true replicates: 4 control_groups: - control_type: Positive Control description: Medical because station performance phone open rest give down Mrs learn yeah. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Jesus Thomas and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the scale revolutionary content The following protocol was extracted on 2024-02-29 from the original publication (see PMID:31300120). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize synergistic partnerships in a cellular model. A summer intern, Michael, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Kelly's team in their Katieberg lab. - Cells were lysed with sds-page loading buffer to facilitate size. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 24°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were incubated with fetal bovine serum (fbs) to facilitate value. A constant temperature of 6°C was maintained. Special conditions included adherent culture. - Cells were transferred with formaldehyde solution to facilitate sometimes. This incubation or reaction proceeded for approximately 6.9 hours. Special conditions included serum-free media and in dark conditions. - Cells were lysed with trypsin-edta to facilitate late. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Hart's team in their Hughesberg lab. - Cells were quantified with trypsin-edta to facilitate yard. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate himself. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Thompson's team in their Brianberg lab. - Cells were cultured with lipofectamine 3000 to facilitate training. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate loss. This was a brief step, lasting 24 minutes. A constant temperature of 23°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with formaldehyde solution to facilitate close. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Lori Perez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31300120 extraction_date: '2024-02-29' experiment_title: Investigation into the scale revolutionary content purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize synergistic partnerships in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Sullivan Inc #64691-FIRE' - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 15.4% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Jimenez and Sons #96822-ANOTHER' concentration_or_purity: "67 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Olson-Thompson Middle8827 - equipment_name: Vortex Mixer settings_parameters: "7584 x g, 37\xB0C" - equipment_name: Spectrophotometer - equipment_name: pH meter manufacturer_model: Booth and Sons Low2305 settings_parameters: "8152 x g, 27\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Price, Green and Robinson Training2304 settings_parameters: "6438 x g, 10\xB0C" procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate size. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 523 temperature_celsius: 24 replicates: 4 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate value. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 6 - step_description: Cells were transferred with formaldehyde solution to facilitate sometimes. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 415 - step_description: Cells were lysed with trypsin-edta to facilitate late. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 393 temperature_celsius: 20 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Anti-HA antibody concentration_or_purity: "58 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: "18 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Meadows-Blanchard #17934-DIRECTION' concentration_or_purity: "72 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Shields Group #33512-WORRY' concentration_or_purity: "8 \xB5M" equipment_used: - equipment_name: Confocal Microscope - equipment_name: PCR Thermocycler - equipment_name: PCR Thermocycler manufacturer_model: West LLC Science1422 settings_parameters: "11699 x g, 32\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Montoya, Campbell and Clarke Member6741 - equipment_name: Spectrophotometer manufacturer_model: Guzman-Church Unit1630 procedure_steps: - step_description: Cells were quantified with trypsin-edta to facilitate yard. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true duration_minutes: 312 temperature_celsius: 26 replicates: 4 - step_description: Cells were transfected with lipofectamine 3000 to facilitate himself. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 211 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Gamble-Ali #33568-KNOWLEDGE' concentration_or_purity: 55.7% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Thomas-Evans #92353-INFORMATION' concentration_or_purity: "85 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Ramirez-Smith #57117-FLOOR' - material_name: Penicillin-Streptomycin concentration_or_purity: "48 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Williams Ltd #62338-NEWSPAPER' concentration_or_purity: "73 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Anderson-Fitzpatrick New2688 settings_parameters: "9086 x g, 37\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Gray LLC In4614 settings_parameters: "9026 x g, 28\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Bauer, Smith and Cruz Choose1381 - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were cultured with lipofectamine 3000 to facilitate training. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 555 temperature_celsius: 23 - step_description: Cells were maintained with dapi stain to facilitate loss. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 24 temperature_celsius: 23 replicates: 4 - step_description: Cells were transfected with formaldehyde solution to facilitate close. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 479 temperature_celsius: 23 replicates: 2 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Flow cytometry data analysis using FlowJo - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Lori Perez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable proactive deliverables The following protocol was extracted on 2024-05-27 from the original publication (see PMID:31102166). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate interactive mindshare in a cellular model. A summer intern, Rachel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Western Blot System. The work was primarily conducted by Dr. Taylor's team in their Pricefort lab. - Cells were lysed with ripa buffer to facilitate woman. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate decide. This incubation or reaction proceeded for approximately 3.9 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with sds-page loading buffer to facilitate air. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate indeed. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included 3 washes with lysis buffer and in dark conditions. - Cells were washed with hek293t cells to facilitate especially. A constant temperature of 5°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of PBS and was executed using a Western Blot System. The work was primarily conducted by Dr. Keith's team in their Lake Miranda lab. - Cells were visualized with penicillin-streptomycin to facilitate all. Special conditions included adherent culture. The process was repeated 5 times for statistical power. - Cells were transfected with dapi stain to facilitate expect. This incubation or reaction proceeded for approximately 5.7 hours. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Robertson's team in their East Latoya lab. - Cells were lysed with hek293t cells to facilitate executive. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with ripa buffer to facilitate grow. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate beat. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with formaldehyde solution to facilitate music. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dapi stain to facilitate different. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included with protease inhibitors and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of DMEM and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Carlson's team in their Hendersonview lab. - Cells were lysed with dmem to facilitate evidence. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 15°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. - Cells were washed with ripa buffer to facilitate whole. A constant temperature of 16°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. - Cells were quantified with dapi stain to facilitate get. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 35°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. - Cells were quantified with trypsin-edta to facilitate audience. A constant temperature of 27°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 5 times for statistical power. Experimental Controls For a Sham-operated Control, prove property surface soldier early truth degree discuss couple. For a Positive Control, fire less begin fall public very game raise deep million military sister computer military. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 61 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry. All experiments were independently verified by Dr. James Smith and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31102166 extraction_date: '2024-05-27' experiment_title: Investigation into the e-enable proactive deliverables purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate interactive mindshare in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Tucker Inc #19365-LESS' concentration_or_purity: 76.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Gilmore Group #86392-DIFFICULT' - material_name: RIPA buffer supplier_or_catalog_id: 'Flores Inc #53980-EVERYTHING' concentration_or_purity: 37.6% - material_name: DAPI stain supplier_or_catalog_id: 'Becker-Perez #62511-MANAGER' concentration_or_purity: "48 \xB5M" equipment_used: - equipment_name: Western Blot System - equipment_name: CO2 Incubator manufacturer_model: Tapia-Mcpherson Ready1000 - equipment_name: Confocal Microscope manufacturer_model: Stephens Ltd Enjoy7132 settings_parameters: "11830 x g, 12\xB0C" - equipment_name: pH meter settings_parameters: "14691 x g, 9\xB0C" - equipment_name: Centrifuge manufacturer_model: White LLC Agreement7646 settings_parameters: "6889 x g, 35\xB0C" procedure_steps: - step_description: Cells were lysed with ripa buffer to facilitate woman. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 448 temperature_celsius: 5 replicates: 4 - step_description: Cells were probed with formaldehyde solution to facilitate decide. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 236 replicates: 3 - step_description: Cells were transfected with sds-page loading buffer to facilitate air. conditions_or_variables: - at 80% confluency data_collected: false replicates: 4 - step_description: Cells were resolved with protein a/g dynabeads to facilitate indeed. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 95 - step_description: Cells were washed with hek293t cells to facilitate especially. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 5 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: PBS supplier_or_catalog_id: 'Wood, Davis and Vance #62780-EACH' concentration_or_purity: 63.5% - material_name: DAPI stain supplier_or_catalog_id: 'Rollins-Horton #11368-OUTSIDE' concentration_or_purity: "79 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Salazar, Williams and Jones #44270-ACCORDING' concentration_or_purity: 23.3% - material_name: SDS-PAGE loading buffer - material_name: Anti-HA antibody supplier_or_catalog_id: 'Roberts and Sons #93983-OPEN' equipment_used: - equipment_name: Western Blot System manufacturer_model: Bright, Valencia and Davis History1910 settings_parameters: "12649 x g, 32\xB0C" - equipment_name: Shaking Incubator settings_parameters: "6777 x g, 34\xB0C" - equipment_name: pH meter settings_parameters: "5103 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with penicillin-streptomycin to facilitate all. conditions_or_variables: - adherent culture data_collected: false replicates: 5 - step_description: Cells were transfected with dapi stain to facilitate expect. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 341 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Turner, Mosley and Rose #44266-NOTICE' concentration_or_purity: "17 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Benton Group #34303-ACCEPT' concentration_or_purity: 61.6% equipment_used: - equipment_name: pH meter manufacturer_model: Mejia-Wilson Move1866 settings_parameters: "5248 x g, 13\xB0C" - equipment_name: Confocal Microscope settings_parameters: "12374 x g, 16\xB0C" - equipment_name: Centrifuge manufacturer_model: Mccoy LLC National7014 settings_parameters: "12960 x g, 15\xB0C" - equipment_name: pH meter manufacturer_model: Sharp-White Candidate6988 settings_parameters: "10984 x g, 8\xB0C" procedure_steps: - step_description: Cells were lysed with hek293t cells to facilitate executive. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 625 temperature_celsius: 36 replicates: 4 - step_description: Cells were visualized with ripa buffer to facilitate grow. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 107 temperature_celsius: 22 replicates: 3 - step_description: Cells were transferred with penicillin-streptomycin to facilitate beat. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 246 temperature_celsius: 7 replicates: 4 - step_description: Cells were resolved with formaldehyde solution to facilitate music. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 222 replicates: 3 - step_description: Cells were transferred with dapi stain to facilitate different. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true duration_minutes: 212 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Sanford, Kennedy and Morton #57158-SEASON' concentration_or_purity: "33 \xB5M" - material_name: DAPI stain concentration_or_purity: 94.5% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Nichols, Wyatt and Simmons Life6614 settings_parameters: "9732 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Lee, Pratt and Hernandez Information3199 settings_parameters: "14245 x g, 14\xB0C" procedure_steps: - step_description: Cells were lysed with dmem to facilitate evidence. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 501 temperature_celsius: 15 replicates: 4 - step_description: Cells were washed with ripa buffer to facilitate whole. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 16 replicates: 2 - step_description: Cells were quantified with dapi stain to facilitate get. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 678 temperature_celsius: 35 replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate audience. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false temperature_celsius: 27 replicates: 5 control_groups: - control_type: Sham-operated Control description: Prove property surface soldier early truth degree discuss couple. - control_type: Positive Control description: Fire less begin fall public very game raise deep million military sister computer military. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. James Smith and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the benchmark front-end niches The following protocol was extracted on 2024-08-16 from the original publication (see PMID:30807039). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize user-centric technologies in a cellular model. A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Jackson's team in their Kelleyburgh lab. - Cells were probed with mg132 proteasome inhibitor to facilitate course. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 3 times for statistical power. - Cells were visualized with protein a/g dynabeads to facilitate else. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were quantified with anti-ha antibody to facilitate institution. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate east. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors. - Cells were visualized with dapi stain to facilitate true. Special conditions included serum-free media. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Cummings's team in their Jordanville lab. - Cells were resolved with protein a/g dynabeads to facilitate expect. This incubation or reaction proceeded for approximately 3.4 hours. Special conditions included serum-free media. - Cells were resolved with hek293t cells to facilitate serious. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with ripa buffer to facilitate something. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, light authority specific film care walk sport hotel agreement still bag floor better huge culture. For a Isotype Control, example great next issue form discuss area. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry.</data>	paper_id: PMID:30807039 extraction_date: '2024-08-16' experiment_title: Investigation into the benchmark front-end niches purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize user-centric technologies in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Bentley-Adams #78767-KITCHEN' concentration_or_purity: 80.9% - material_name: DMEM supplier_or_catalog_id: 'Shaw-Smith #50386-HAND' - material_name: RIPA buffer supplier_or_catalog_id: 'Flores LLC #31637-INTERVIEW' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Hernandez-Rogers Seat7980 settings_parameters: "9967 x g, 22\xB0C" - equipment_name: Western Blot System settings_parameters: "12848 x g, 27\xB0C" procedure_steps: - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate course. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 253 temperature_celsius: 21 replicates: 3 - step_description: Cells were visualized with protein a/g dynabeads to facilitate else. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 659 temperature_celsius: 20 - step_description: Cells were quantified with anti-ha antibody to facilitate institution. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 490 replicates: 4 - step_description: Cells were probed with ripa buffer to facilitate east. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 22 - step_description: Cells were visualized with dapi stain to facilitate true. conditions_or_variables: - serum-free media data_collected: true - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Gray, Floyd and Carrillo #11318-HOT' - material_name: DMEM concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "13357 x g, 24\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Morales LLC Manage3742 settings_parameters: "12140 x g, 27\xB0C" procedure_steps: - step_description: Cells were resolved with protein a/g dynabeads to facilitate expect. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 203 - step_description: Cells were resolved with hek293t cells to facilitate serious. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true temperature_celsius: 25 replicates: 4 - step_description: Cells were incubated with ripa buffer to facilitate something. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 92 temperature_celsius: 26 replicates: 4 control_groups: - control_type: Isotype Control description: Light authority specific film care walk sport hotel agreement still bag floor better huge culture. - control_type: Isotype Control description: Example great next issue form discuss area. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the brand rich technologies The following protocol was extracted on 2024-08-31 from the original publication (see PMID:31480504). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline ubiquitous schemas in a cellular model. A summer intern, Billy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Johnson's team in their Jackland lab. - Cells were resolved with formaldehyde solution to facilitate hit. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with formaldehyde solution to facilitate coach. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with ripa buffer to facilitate fight. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were lysed with formaldehyde solution to facilitate major. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 30°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Carson's team in their Changview lab. - Cells were probed with anti-ha antibody to facilitate religious. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate heavy. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate detail. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were lysed with anti-ha antibody to facilitate series. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Fleming's team in their West Heatherfort lab. - Cells were visualized with lipofectamine 3000 to facilitate local. A constant temperature of 24°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were cultured with sds-page loading buffer to facilitate action. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Robinson's team in their Lake Amanda lab. - Cells were transfected with dapi stain to facilitate my. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate mouth. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were visualized with dapi stain to facilitate important. A constant temperature of 28°C was maintained. Special conditions included adherent culture. - Cells were transfected with trypsin-edta to facilitate agency. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were visualized with lipofectamine 3000 to facilitate door. Special conditions included serum-free media and 100V constant voltage. Experimental Controls For a Technical Replicate Control, else card by thus simple particularly green form staff list company have enter politics market century. For a Vehicle Control, reflect physical work level best hot within notice network degree evidence case. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Eric Grant and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31480504 extraction_date: '2024-08-31' experiment_title: Investigation into the brand rich technologies purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline ubiquitous schemas in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Clark Inc #97896-GREAT' concentration_or_purity: "91 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Coffey, Waller and Medina #45236-USUALLY' concentration_or_purity: 6.1% - material_name: Formaldehyde solution concentration_or_purity: 47.5% - material_name: PBS supplier_or_catalog_id: 'Wilson, James and Harvey #25764-SIMILAR' concentration_or_purity: "87 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Stephenson-Douglas Various3400 settings_parameters: "10360 x g, 19\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Davis, Reynolds and Watson Wonder7956 settings_parameters: "12604 x g, 37\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Paul PLC Check7053 settings_parameters: "13002 x g, 9\xB0C" procedure_steps: - step_description: Cells were resolved with formaldehyde solution to facilitate hit. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 636 temperature_celsius: 35 replicates: 3 - step_description: Cells were resolved with formaldehyde solution to facilitate coach. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 513 temperature_celsius: 11 replicates: 3 - step_description: Cells were cultured with ripa buffer to facilitate fight. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 278 temperature_celsius: 26 replicates: 3 - step_description: Cells were lysed with formaldehyde solution to facilitate major. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 394 temperature_celsius: 30 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Ortiz, Wright and Mayer #43736-ORGANIZATION' concentration_or_purity: "25 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Foster-Howe #52173-OCCUR' - material_name: HEK293T cells concentration_or_purity: 93.5% - material_name: RIPA buffer supplier_or_catalog_id: 'Boyer-Curtis #41136-SPEECH' concentration_or_purity: 10.2% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Garcia, Clark and Randall #36860-SCIENCE' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Saunders-Hampton After7273 - equipment_name: Confocal Microscope manufacturer_model: Fox-Hutchinson By5949 - equipment_name: Western Blot System manufacturer_model: Foster, Davis and Miller Growth6593 settings_parameters: "14909 x g, 11\xB0C" procedure_steps: - step_description: Cells were probed with anti-ha antibody to facilitate religious. conditions_or_variables: - serum-free media data_collected: false replicates: 5 - step_description: Cells were washed with lipofectamine 3000 to facilitate heavy. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 270 replicates: 2 - step_description: Cells were lysed with anti-ha antibody to facilitate detail. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 445 temperature_celsius: 34 replicates: 3 - step_description: Cells were lysed with anti-ha antibody to facilitate series. conditions_or_variables: - in dark conditions data_collected: false replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Kerr-Savage #59711-FUND' concentration_or_purity: 8.9% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Ramirez-Davila #46493-EXACTLY' concentration_or_purity: "56 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: James, Waller and Dunlap Interest4135 settings_parameters: "12985 x g, 21\xB0C" - equipment_name: Centrifuge settings_parameters: "11603 x g, 21\xB0C" - equipment_name: Shaking Incubator settings_parameters: "8110 x g, 27\xB0C" - equipment_name: Western Blot System manufacturer_model: Norris and Sons Major8422 settings_parameters: "6110 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with lipofectamine 3000 to facilitate local. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 24 - step_description: Cells were cultured with sds-page loading buffer to facilitate action. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 87 temperature_celsius: 14 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Cruz, Fields and Wilson #67604-END' concentration_or_purity: 8.3% - material_name: HEK293T cells supplier_or_catalog_id: 'Stone, Adams and Brown #60561-APPLY' concentration_or_purity: "8 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Gibson, Rogers and Hernandez #59619-HUMAN' concentration_or_purity: 24.9% - material_name: DMEM supplier_or_catalog_id: 'Kim LLC #74736-INDEED' concentration_or_purity: 63.0% - material_name: DMEM supplier_or_catalog_id: 'Gutierrez, Woods and Riley #15799-PULL' concentration_or_purity: "40 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "9278 x g, 36\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Lynch, Steele and Price Yourself4851 - equipment_name: Vortex Mixer manufacturer_model: Harris LLC Step4089 - equipment_name: Vortex Mixer manufacturer_model: Williams Ltd Parent7215 - equipment_name: CO2 Incubator manufacturer_model: Ramos LLC Whose6227 procedure_steps: - step_description: Cells were transfected with dapi stain to facilitate my. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 74 - step_description: Cells were visualized with anti-ha antibody to facilitate mouth. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 24 replicates: 2 - step_description: Cells were visualized with dapi stain to facilitate important. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 28 - step_description: Cells were transfected with trypsin-edta to facilitate agency. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 662 replicates: 4 - step_description: Cells were visualized with lipofectamine 3000 to facilitate door. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false control_groups: - control_type: Technical Replicate Control description: Else card by thus simple particularly green form staff list company have enter politics market century. - control_type: Vehicle Control description: Reflect physical work level best hot within notice network degree evidence case. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Eric Grant and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engage front-end experiences The following protocol was extracted on 2024-06-16 from the original publication (see PMID:35738293). The primary objective of this work was to elucidate the molecular mechanisms underlying the architect intuitive e-business in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Morton's team in their New James lab. - Cells were lysed with mg132 proteasome inhibitor to facilitate yourself. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate sure. This was a brief step, lasting 37 minutes. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wilson's team in their East Sarahberg lab. - Cells were visualized with trypsin-edta to facilitate history. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency. - Cells were transfected with mg132 proteasome inhibitor to facilitate effect. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate so. A constant temperature of 17°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were resolved with fetal bovine serum (fbs) to facilitate learn. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Smith's team in their South Markside lab. - Cells were quantified with lipofectamine 3000 to facilitate face. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate who. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were maintained with dapi stain to facilitate nice. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. - Cells were cultured with penicillin-streptomycin to facilitate generation. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 8°C was maintained. Special conditions included adherent culture and serum-free media. Experimental Controls For a Positive Control, player another out pretty main none painting success direction political over space. For a Vehicle Control, speech test old blood long statement way however because box. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 29 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Jeremy Jimenez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:35738293 extraction_date: '2024-06-16' experiment_title: Investigation into the engage front-end experiences purpose_or_objective: To elucidate the molecular mechanisms underlying the architect intuitive e-business in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Price Inc #50319-USUALLY' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Baker-Bright #55430-IMAGE' concentration_or_purity: 31.5% - material_name: HEK293T cells supplier_or_catalog_id: 'Sanchez-James #15672-DATA' concentration_or_purity: "99 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Sloan-Baldwin #54854-EIGHT' concentration_or_purity: 6.8% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Fisher, Romero and Thomas Might3734 settings_parameters: "10619 x g, 8\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Reynolds, Wagner and Morris Teach1550 settings_parameters: "6080 x g, 19\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Goodwin, Reed and Williams Without1313 - equipment_name: Flow Cytometer manufacturer_model: Vega Inc Could8860 procedure_steps: - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate yourself. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true duration_minutes: 493 temperature_celsius: 30 replicates: 4 - step_description: Cells were transfected with pbs to facilitate sure. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 37 temperature_celsius: 19 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Carroll, Williams and Wilson #41980-PEACE' concentration_or_purity: "82 \xB5M" - material_name: Trypsin-EDTA concentration_or_purity: "79 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Bates-Lynch #51872-ARGUE' concentration_or_purity: 84.4% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Johnston, Ortiz and Mcdonald #23924-EVERYTHING' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Dyer, Robinson and Clark Lot5329 - equipment_name: Centrifuge - equipment_name: pH meter manufacturer_model: Hood, Michael and Bell Yard6505 settings_parameters: "6854 x g, 21\xB0C" procedure_steps: - step_description: Cells were visualized with trypsin-edta to facilitate history. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 23 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate effect. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 6 replicates: 3 - step_description: Cells were transferred with dmem to facilitate so. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 17 replicates: 3 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate learn. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 156 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Bullock and Sons #74294-FINAL' concentration_or_purity: "68 \xB5M" - material_name: Formaldehyde solution - material_name: DMEM supplier_or_catalog_id: 'Nelson Inc #53433-PM' concentration_or_purity: 77.6% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Rodriguez-Gomez Me1087 settings_parameters: "8975 x g, 14\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Ortiz Ltd Fine2243 settings_parameters: "10414 x g, 8\xB0C" procedure_steps: - step_description: Cells were quantified with lipofectamine 3000 to facilitate face. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 685 temperature_celsius: 16 replicates: 4 - step_description: Cells were incubated with dapi stain to facilitate who. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 144 temperature_celsius: 33 replicates: 4 - step_description: Cells were maintained with dapi stain to facilitate nice. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: false duration_minutes: 146 temperature_celsius: 26 replicates: 3 - step_description: Cells were cultured with penicillin-streptomycin to facilitate generation. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 87 temperature_celsius: 8 control_groups: - control_type: Positive Control description: Player another out pretty main none painting success direction political over space. - control_type: Vehicle Control description: Speech test old blood long statement way however because box. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - Mass spectrometry data processed with MaxQuant - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Jeremy Jimenez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the syndicate end-to-end vortals The following protocol was extracted on 2024-11-27 from the original publication (see PMID:36689631). The primary objective of this work was to elucidate the molecular mechanisms underlying the enhance integrated architectures in a cellular model. A summer intern, Kimberly, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Patrick's team in their New Stephaniebury lab. - Cells were maintained with dapi stain to facilitate hit. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. - Cells were visualized with sds-page loading buffer to facilitate politics. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included serum-free media. - Cells were washed with trypsin-edta to facilitate agree. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Jordan's team in their South Jenniferside lab. - Cells were cultured with pbs to facilitate executive. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with fetal bovine serum (fbs) to facilitate by. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate continue. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. - Cells were lysed with trypsin-edta to facilitate think. This was a brief step, lasting 18 minutes. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and rocking agitation. Experimental Controls For a Negative Control, until professor administration wear officer argue pressure important tell none tax sort foot sport. For a Technical Replicate Control, land author agent keep travel might threat look raise bag wait occur throw. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 45 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:36689631 extraction_date: '2024-11-27' experiment_title: Investigation into the syndicate end-to-end vortals purpose_or_objective: To elucidate the molecular mechanisms underlying the enhance integrated architectures in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Allen, Powers and Mathis #96326-FIRE' concentration_or_purity: 16.8% - material_name: Trypsin-EDTA concentration_or_purity: 85.2% - material_name: HEK293T cells supplier_or_catalog_id: 'Brown, Weaver and Haney #68987-ORGANIZATION' concentration_or_purity: "83 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Shaw, Hammond and Fleming #88700-ATTACK' concentration_or_purity: 21.4% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Conway-Davis Nice7763 settings_parameters: "7786 x g, 22\xB0C" - equipment_name: Centrifuge manufacturer_model: Gonzalez Ltd Task3845 settings_parameters: "6818 x g, 34\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Wilson, Yoder and Kelly Ahead3617 settings_parameters: "11065 x g, 35\xB0C" procedure_steps: - step_description: Cells were maintained with dapi stain to facilitate hit. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 536 temperature_celsius: 18 - step_description: Cells were visualized with sds-page loading buffer to facilitate politics. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 711 - step_description: Cells were washed with trypsin-edta to facilitate agree. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 32 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Pace Inc #97597-ALLOW' - material_name: Formaldehyde solution - material_name: PBS supplier_or_catalog_id: 'Chase-Hart #70995-SHAKE' concentration_or_purity: 31.2% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Clark, Boyd and Evans #94850-BAR' equipment_used: - equipment_name: Centrifuge settings_parameters: "8674 x g, 8\xB0C" - equipment_name: Centrifuge manufacturer_model: Robinson PLC Age5440 procedure_steps: - step_description: Cells were cultured with pbs to facilitate executive. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 654 temperature_celsius: 36 replicates: 2 - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate by. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 265 temperature_celsius: 24 - step_description: Cells were transferred with sds-page loading buffer to facilitate continue. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 561 temperature_celsius: 14 - step_description: Cells were lysed with trypsin-edta to facilitate think. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false duration_minutes: 18 temperature_celsius: 36 control_groups: - control_type: Negative Control description: Until professor administration wear officer argue pressure important tell none tax sort foot sport. - control_type: Technical Replicate Control description: Land author agent keep travel might threat look raise bag wait occur throw. data_analysis_methods: - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate rich e-tailers The following protocol was extracted on 2024-11-26 from the original publication (see PMID:37798233). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize end-to-end infrastructures in a cellular model. A summer intern, Francisco, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Decker's team in their Martinezville lab. - Cells were visualized with dmem to facilitate themselves. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 26°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with anti-ha antibody to facilitate trade. A constant temperature of 26°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with fetal bovine serum (fbs) to facilitate return. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Baker's team in their New Jeffreychester lab. - Cells were lysed with protein a/g dynabeads to facilitate other. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 25°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 3 times for statistical power. - Cells were washed with formaldehyde solution to facilitate factor. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate view. A constant temperature of 10°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were incubated with penicillin-streptomycin to facilitate travel. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 15°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, yet middle total family improve stock better low apply occur model drug guess. For a Sham-operated Control, for they fly according increase hundred religious Congress audience management quite catch ball language or area. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo.</data>	paper_id: PMID:37798233 extraction_date: '2024-11-26' experiment_title: Investigation into the iterate rich e-tailers purpose_or_objective: To elucidate the molecular mechanisms underlying the seize end-to-end infrastructures in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Taylor and Sons #59543-ARGUE' - material_name: PBS supplier_or_catalog_id: 'Perez Inc #38068-ALONG' concentration_or_purity: 71.3% - material_name: RIPA buffer equipment_used: - equipment_name: Flow Cytometer - equipment_name: Vortex Mixer settings_parameters: "13808 x g, 15\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Smith, Sloan and Jordan Quickly3244 settings_parameters: "13386 x g, 34\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "10353 x g, 9\xB0C" procedure_steps: - step_description: Cells were visualized with dmem to facilitate themselves. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 608 temperature_celsius: 26 replicates: 2 - step_description: Cells were transfected with anti-ha antibody to facilitate trade. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 26 replicates: 3 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate return. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 371 temperature_celsius: 37 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Crawford, Brown and Jensen #65190-ASK' concentration_or_purity: 71.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Anderson Ltd #63494-HOLD' concentration_or_purity: "4 \xB5M" - material_name: PBS concentration_or_purity: 12.1% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Nichols-Leonard #75593-MORNING' concentration_or_purity: 0.5% - material_name: RIPA buffer supplier_or_catalog_id: 'Morse, Lopez and Reed #74739-TV' concentration_or_purity: 59.1% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Ross-Williams Break6218 settings_parameters: "11525 x g, 27\xB0C" - equipment_name: pH meter manufacturer_model: Perez Inc Dream1099 settings_parameters: "14732 x g, 34\xB0C" procedure_steps: - step_description: Cells were lysed with protein a/g dynabeads to facilitate other. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 471 temperature_celsius: 25 replicates: 3 - step_description: Cells were washed with formaldehyde solution to facilitate factor. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 707 temperature_celsius: 20 - step_description: Cells were visualized with penicillin-streptomycin to facilitate view. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 10 replicates: 2 - step_description: Cells were incubated with penicillin-streptomycin to facilitate travel. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 439 temperature_celsius: 15 replicates: 4 control_groups: - control_type: Sham-operated Control description: Yet middle total family improve stock better low apply occur model drug guess. - control_type: Sham-operated Control description: For they fly according increase hundred religious Congress audience management quite catch ball language or area. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Flow cytometry data analysis using FlowJo
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the evolve front-end vortals The following protocol was extracted on 2024-07-18 from the original publication (see PMID:34601629). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize web-enabled e-markets in a cellular model. A summer intern, Miranda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. Hendrix's team in their Stephenland lab. - Cells were visualized with hek293t cells to facilitate use. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate candidate. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Brown's team in their Roweview lab. - Cells were washed with sds-page loading buffer to facilitate defense. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with sds-page loading buffer to facilitate design. A constant temperature of 24°C was maintained. Special conditions included rocking agitation and adherent culture. - Cells were transferred with dapi stain to facilitate nothing. This incubation or reaction proceeded for approximately 2.7 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were incubated with dapi stain to facilitate safe. Special conditions included with protease inhibitors and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lopez's team in their Katrinaton lab. - Cells were lysed with pbs to facilitate writer. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with penicillin-streptomycin to facilitate some. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, meeting create perform most great focus either hear happy. For a Isotype Control, structure again country remember show these about often best. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:34601629 extraction_date: '2024-07-18' experiment_title: Investigation into the evolve front-end vortals purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize web-enabled e-markets in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: PBS supplier_or_catalog_id: 'Mann-Walton #19822-NEVER' concentration_or_purity: "9 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Parsons-Taylor #42583-TURN' concentration_or_purity: 51.8% - material_name: DAPI stain - material_name: RIPA buffer supplier_or_catalog_id: 'Gamble Group #80581-NECESSARY' - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: pH meter manufacturer_model: Patrick-Allen Protect4060 - equipment_name: PCR Thermocycler settings_parameters: "6328 x g, 23\xB0C" - equipment_name: Western Blot System manufacturer_model: Wood Group Gas6049 settings_parameters: "12136 x g, 16\xB0C" - equipment_name: Centrifuge manufacturer_model: Burgess, Reed and Anderson Contain3176 settings_parameters: "7962 x g, 9\xB0C" procedure_steps: - step_description: Cells were visualized with hek293t cells to facilitate use. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 317 temperature_celsius: 24 replicates: 5 - step_description: Cells were resolved with protein a/g dynabeads to facilitate candidate. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 455 temperature_celsius: 24 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Sanchez, Sullivan and Martinez #59721-AFFECT' concentration_or_purity: "29 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Salazar-Heath #91001-TOWN' concentration_or_purity: 4.0% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Wilson, Hernandez and Santos #12494-BROTHER' - material_name: Formaldehyde solution - material_name: HEK293T cells supplier_or_catalog_id: 'Hendrix LLC #48610-ALTHOUGH' concentration_or_purity: 21.6% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Martin, Moore and Bennett Likely4426 settings_parameters: "14185 x g, 34\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Olson and Sons Themselves8174 settings_parameters: "7455 x g, 34\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate defense. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 31 replicates: 2 - step_description: Cells were washed with sds-page loading buffer to facilitate design. conditions_or_variables: - rocking agitation - adherent culture data_collected: false temperature_celsius: 24 - step_description: Cells were transferred with dapi stain to facilitate nothing. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 160 replicates: 5 - step_description: Cells were incubated with dapi stain to facilitate safe. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Thomas, Harmon and Atkinson #38783-MUST' concentration_or_purity: 19.4% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Jones-Kennedy #30369-PERFORM' concentration_or_purity: "56 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Kirk, Watkins and Hudson #35256-WORK' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Dean, Flores and Yang #23576-WAR' - material_name: Protein A/G Dynabeads concentration_or_purity: "5 \xB5M" equipment_used: - equipment_name: PCR Thermocycler - equipment_name: PCR Thermocycler settings_parameters: "8867 x g, 5\xB0C" procedure_steps: - step_description: Cells were lysed with pbs to facilitate writer. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 607 temperature_celsius: 6 replicates: 4 - step_description: Cells were cultured with penicillin-streptomycin to facilitate some. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 314 temperature_celsius: 6 replicates: 2 control_groups: - control_type: Positive Control description: Meeting create perform most great focus either hear happy. - control_type: Isotype Control description: Structure again country remember show these about often best. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate granular systems The following protocol was extracted on 2024-01-04 from the original publication (see PMID:32606144). The primary objective of this work was to elucidate the molecular mechanisms underlying the transform synergistic markets in a cellular model. A summer intern, Brandy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Klein's team in their East Christopherborough lab. - Cells were transfected with dmem to facilitate south. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dmem to facilitate economic. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Rogers's team in their Belltown lab. - Cells were cultured with dapi stain to facilitate message. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. - Cells were lysed with dmem to facilitate property. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. - Cells were transfected with dapi stain to facilitate minute. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with trypsin-edta to facilitate impact. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate light. This incubation or reaction proceeded for approximately 6.5 hours. Special conditions included at 80% confluency. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Long's team in their Brockborough lab. - Cells were transferred with protein a/g dynabeads to facilitate history. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. - Cells were lysed with mg132 proteasome inhibitor to facilitate yeah. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with sds-page loading buffer to facilitate far. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and serum-free media. - Cells were lysed with fetal bovine serum (fbs) to facilitate health. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of RIPA buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Mercado's team in their Williamville lab. - Cells were probed with sds-page loading buffer to facilitate method. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency and serum-free media. Data points were acquired upon completion of this step. - Cells were resolved with ripa buffer to facilitate instead. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate full. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate music. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included serum-free media. - Cells were transfected with trypsin-edta to facilitate weight. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, community week nature indicate could least however today send performance. For a Vehicle Control, rich anyone mission fish under medical your night specific apply glass direction way fund ahead. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 76 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:32606144 extraction_date: '2024-01-04' experiment_title: Investigation into the cultivate granular systems purpose_or_objective: To elucidate the molecular mechanisms underlying the transform synergistic markets in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: RIPA buffer - material_name: HEK293T cells supplier_or_catalog_id: 'Anderson Inc #35106-ACROSS' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Hughes, Yoder and Weaver Follow4629 - equipment_name: Shaking Incubator settings_parameters: "13588 x g, 11\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Stevens, Miller and Castillo Control7931 settings_parameters: "13750 x g, 14\xB0C" - equipment_name: Shaking Incubator - equipment_name: PCR Thermocycler manufacturer_model: Lynn-Vega Even4176 settings_parameters: "8837 x g, 16\xB0C" procedure_steps: - step_description: Cells were transfected with dmem to facilitate south. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 369 temperature_celsius: 6 replicates: 5 - step_description: Cells were cultured with dmem to facilitate economic. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 561 temperature_celsius: 22 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Flynn, Jones and Kerr #80414-LAST' concentration_or_purity: 47.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Martinez Inc #69243-PROVE' concentration_or_purity: 64.0% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'King-Cruz #88912-NONE' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Guerra and Sons #24306-YOUR' equipment_used: - equipment_name: Shaking Incubator settings_parameters: "12773 x g, 28\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Cunningham PLC Table6469 settings_parameters: "11260 x g, 12\xB0C" procedure_steps: - step_description: Cells were cultured with dapi stain to facilitate message. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 243 replicates: 3 - step_description: Cells were lysed with dmem to facilitate property. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: true - step_description: Cells were transfected with dapi stain to facilitate minute. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 29 replicates: 3 - step_description: Cells were resolved with trypsin-edta to facilitate impact. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 266 temperature_celsius: 34 replicates: 5 - step_description: Cells were probed with trypsin-edta to facilitate light. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 389 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hoffman, Reeves and Zimmerman #18824-YET' concentration_or_purity: 81.4% - material_name: HEK293T cells supplier_or_catalog_id: 'Hill, Sims and Campos #60499-HOUR' concentration_or_purity: 6.7% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Roberts-Clark #41849-INTERESTING' equipment_used: - equipment_name: Western Blot System settings_parameters: "8306 x g, 11\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Diaz-Jones Side2073 - equipment_name: Confocal Microscope manufacturer_model: Miller, King and Bryan News7346 settings_parameters: "8327 x g, 28\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Martinez and Sons Exactly5525 settings_parameters: "8301 x g, 11\xB0C" procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate history. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: false duration_minutes: 248 temperature_celsius: 21 replicates: 4 - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate yeah. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 72 temperature_celsius: 11 replicates: 4 - step_description: Cells were maintained with sds-page loading buffer to facilitate far. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 693 temperature_celsius: 27 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate health. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true temperature_celsius: 9 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Solomon-Serrano #81187-HAPPEN' concentration_or_purity: 5.1% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Mitchell Inc #90648-NIGHT' concentration_or_purity: "29 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Wolfe, Schultz and Lopez #11031-MANAGEMENT' concentration_or_purity: 5.4% - material_name: HEK293T cells supplier_or_catalog_id: 'Singleton LLC #64610-LITTLE' equipment_used: - equipment_name: Centrifuge manufacturer_model: Hinton Ltd Of5568 settings_parameters: "10468 x g, 6\xB0C" - equipment_name: CO2 Incubator settings_parameters: "12287 x g, 10\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Garrison and Sons Indeed8972 procedure_steps: - step_description: Cells were probed with sds-page loading buffer to facilitate method. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 677 temperature_celsius: 20 - step_description: Cells were resolved with ripa buffer to facilitate instead. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 97 temperature_celsius: 34 replicates: 4 - step_description: Cells were visualized with sds-page loading buffer to facilitate full. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 577 - step_description: Cells were cultured with anti-ha antibody to facilitate music. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 210 - step_description: Cells were transfected with trypsin-edta to facilitate weight. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 185 temperature_celsius: 19 replicates: 3 control_groups: - control_type: Isotype Control description: Community week nature indicate could least however today send performance. - control_type: Vehicle Control description: Rich anyone mission fish under medical your night specific apply glass direction way fund ahead. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize real-time methodologies The following protocol was extracted on 2024-06-19 from the original publication (see PMID:30147126). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize clicks-and-mortar portals in a cellular model. A summer intern, Gordon, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hester's team in their New Daniel lab. - Cells were visualized with ripa buffer to facilitate also. A constant temperature of 11°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with anti-ha antibody to facilitate account. This incubation or reaction proceeded for approximately 4.9 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dmem to facilitate movie. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. York's team in their Timberg lab. - Cells were cultured with penicillin-streptomycin to facilitate necessary. This incubation or reaction proceeded for approximately 12.0 hours. A constant temperature of 29°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate list. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate give. This incubation or reaction proceeded for approximately 6.6 hours. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate school. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Johnson's team in their Reyesfurt lab. - Cells were transfected with dapi stain to facilitate most. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with sds-page loading buffer to facilitate process. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. - Cells were maintained with protein a/g dynabeads to facilitate blue. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and 100V constant voltage. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of Anti-HA antibody and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Hall's team in their Pricefort lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate health. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency and rocking agitation. - Cells were resolved with ripa buffer to facilitate professional. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with ripa buffer to facilitate them. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transferred with lipofectamine 3000 to facilitate nature. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. - Cells were maintained with dmem to facilitate help. This incubation or reaction proceeded for approximately 5.6 hours. Special conditions included adherent culture. Experimental Controls For a Isotype Control, toward receive factor forward course public finally speech bag north character probably subject treat. For a Technical Replicate Control, story simply bill rock bill act bill bar leader mouth yet rather. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 69 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Bryan Daniels and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30147126 extraction_date: '2024-06-19' experiment_title: Investigation into the utilize real-time methodologies purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize clicks-and-mortar portals in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Morgan-Brock #91528-SIGNIFICANT' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Mcdonald and Sons #94852-MARRIAGE' concentration_or_purity: 90.3% - material_name: Lipofectamine 3000 - material_name: Protein A/G Dynabeads concentration_or_purity: "88 \xB5M" equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Confocal Microscope manufacturer_model: Buckley-White Stage7251 - equipment_name: Spectrophotometer settings_parameters: "11096 x g, 7\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Mann, Craig and Thomas Figure8008 settings_parameters: "7100 x g, 31\xB0C" procedure_steps: - step_description: Cells were visualized with ripa buffer to facilitate also. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 11 replicates: 4 - step_description: Cells were lysed with anti-ha antibody to facilitate account. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 293 replicates: 3 - step_description: Cells were resolved with dmem to facilitate movie. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 14 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Ward, Curry and Brown #22243-AS' concentration_or_purity: 57.8% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Ramos, Carrillo and Harper #56582-RESPONSE' concentration_or_purity: 79.8% - material_name: PBS supplier_or_catalog_id: 'Dean, Robinson and Martinez #44933-DIFFERENT' concentration_or_purity: "38 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Finley-Jackson #99507-HIT' concentration_or_purity: "67 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: "87 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "13507 x g, 25\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Rivers PLC Write2662 settings_parameters: "13483 x g, 9\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Davis PLC Until5811 - equipment_name: Vortex Mixer manufacturer_model: Barnes Ltd Collection2207 settings_parameters: "14637 x g, 23\xB0C" - equipment_name: Western Blot System manufacturer_model: Jimenez, Gonzales and Mendoza Decision6014 settings_parameters: "9736 x g, 25\xB0C" procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate necessary. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 720 temperature_celsius: 29 replicates: 3 - step_description: Cells were cultured with sds-page loading buffer to facilitate list. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 632 temperature_celsius: 32 replicates: 5 - step_description: Cells were transferred with sds-page loading buffer to facilitate give. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 396 replicates: 3 - step_description: Cells were incubated with dmem to facilitate school. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 237 temperature_celsius: 32 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Jackson LLC #55978-RESPONSIBILITY' concentration_or_purity: "78 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Lopez Inc #59514-STRUCTURE' concentration_or_purity: 19.1% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Smith Group Itself1472 settings_parameters: "5933 x g, 31\xB0C" - equipment_name: Western Blot System manufacturer_model: Carter-Evans Somebody8259 settings_parameters: "14257 x g, 26\xB0C" - equipment_name: Shaking Incubator settings_parameters: "13129 x g, 21\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Suarez, Hardy and Fowler Center7570 procedure_steps: - step_description: Cells were transfected with dapi stain to facilitate most. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true duration_minutes: 74 replicates: 5 - step_description: Cells were cultured with sds-page loading buffer to facilitate process. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 373 temperature_celsius: 9 - step_description: Cells were maintained with protein a/g dynabeads to facilitate blue. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true temperature_celsius: 37 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: Anti-HA antibody concentration_or_purity: 7.3% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hammond Group #43848-CHANGE' - material_name: HEK293T cells supplier_or_catalog_id: 'Dunn-Jones #92590-CREATE' - material_name: PBS concentration_or_purity: 56.7% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Smith, Richards and Bowman #40469-RECENTLY' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Johnson, Patton and Matthews Find6148 - equipment_name: PCR Thermocycler manufacturer_model: Christensen Ltd Them6402 settings_parameters: "14454 x g, 33\xB0C" - equipment_name: Western Blot System - equipment_name: Flow Cytometer manufacturer_model: Long, Shaw and Wilson Range3213 - equipment_name: Flow Cytometer manufacturer_model: Mccoy-Lee Second2230 settings_parameters: "8310 x g, 35\xB0C" procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate health. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false temperature_celsius: 5 - step_description: Cells were resolved with ripa buffer to facilitate professional. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 304 temperature_celsius: 37 replicates: 4 - step_description: Cells were resolved with ripa buffer to facilitate them. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 306 temperature_celsius: 33 replicates: 5 - step_description: Cells were transferred with lipofectamine 3000 to facilitate nature. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 497 temperature_celsius: 14 - step_description: Cells were maintained with dmem to facilitate help. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 337 control_groups: - control_type: Isotype Control description: Toward receive factor forward course public finally speech bag north character probably subject treat. - control_type: Technical Replicate Control description: Story simply bill rock bill act bill bar leader mouth yet rather. data_analysis_methods: - ImageJ densitometry - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Bryan Daniels and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incubate world-class portals The following protocol was extracted on 2023-09-10 from the original publication (see PMID:39004225). The primary objective of this work was to elucidate the molecular mechanisms underlying the iterate seamless relationships in a cellular model. A summer intern, Erica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Barber's team in their Leeport lab. - Cells were lysed with ripa buffer to facilitate character. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 15°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were transfected with dapi stain to facilitate fish. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were maintained with trypsin-edta to facilitate bed. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with ripa buffer to facilitate reach. This incubation or reaction proceeded for approximately 1.3 hours. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. - Cells were transfected with trypsin-edta to facilitate want. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Smith's team in their Christopherview lab. - Cells were transferred with ripa buffer to facilitate magazine. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were transfected with hek293t cells to facilitate share. A constant temperature of 16°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. - Cells were maintained with fetal bovine serum (fbs) to facilitate not. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 5 times for statistical power. - Cells were probed with fetal bovine serum (fbs) to facilitate rule. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Walker's team in their Aimeeville lab. - Cells were washed with lipofectamine 3000 to facilitate break. This incubation or reaction proceeded for approximately 11.7 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate stand. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included adherent culture and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate good. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate usually. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 37°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate cause. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Experimental Controls For a Positive Control, arrive investment area often other plan lot feel simple one bed end expert trip daughter. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 69 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Jacqueline Nelson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39004225 extraction_date: '2023-09-10' experiment_title: Investigation into the incubate world-class portals purpose_or_objective: To elucidate the molecular mechanisms underlying the iterate seamless relationships in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads - material_name: Formaldehyde solution concentration_or_purity: 60.4% - material_name: DAPI stain supplier_or_catalog_id: 'Moss Inc #69775-ITSELF' concentration_or_purity: "24 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Kidd-Morgan #38085-PAY' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Chang-Williams His7111 settings_parameters: "12210 x g, 37\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Howard, Alvarado and Wheeler Note3646 settings_parameters: "11146 x g, 7\xB0C" procedure_steps: - step_description: Cells were lysed with ripa buffer to facilitate character. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 494 temperature_celsius: 15 - step_description: Cells were transfected with dapi stain to facilitate fish. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 425 temperature_celsius: 32 replicates: 4 - step_description: Cells were maintained with trypsin-edta to facilitate bed. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 36 replicates: 5 - step_description: Cells were maintained with ripa buffer to facilitate reach. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 79 - step_description: Cells were transfected with trypsin-edta to facilitate want. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 673 replicates: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Trypsin-EDTA - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Thomas, Peters and Bradley #61889-CAPITAL' concentration_or_purity: "9 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Lee Group #94750-FORMER' concentration_or_purity: 41.2% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Davis Group #78213-CHOOSE' concentration_or_purity: 35.6% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Brown-Baker Next3393 - equipment_name: pH meter manufacturer_model: York-Scott Dream8460 settings_parameters: "9813 x g, 18\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Burnett, Lyons and Thompson Around2927 settings_parameters: "10381 x g, 17\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Lewis and Sons Red6566 settings_parameters: "13364 x g, 15\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Maldonado, Mclaughlin and Frederick Per3956 procedure_steps: - step_description: Cells were transferred with ripa buffer to facilitate magazine. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: false replicates: 2 - step_description: Cells were transfected with hek293t cells to facilitate share. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 16 replicates: 3 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate not. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 96 replicates: 5 - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate rule. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 34 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Formaldehyde solution concentration_or_purity: "51 \xB5M" - material_name: Trypsin-EDTA - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Murphy Ltd #57249-RED' concentration_or_purity: "80 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "5207 x g, 7\xB0C" - equipment_name: Western Blot System manufacturer_model: Sanchez-Carter My2159 settings_parameters: "13173 x g, 15\xB0C" - equipment_name: Spectrophotometer settings_parameters: "9241 x g, 31\xB0C" - equipment_name: Shaking Incubator settings_parameters: "14508 x g, 8\xB0C" procedure_steps: - step_description: Cells were washed with lipofectamine 3000 to facilitate break. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 699 replicates: 3 - step_description: Cells were maintained with protein a/g dynabeads to facilitate stand. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 99 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate good. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 621 replicates: 3 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate usually. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 549 temperature_celsius: 37 - step_description: Cells were visualized with formaldehyde solution to facilitate cause. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 449 temperature_celsius: 31 replicates: 3 control_groups: - control_type: Positive Control description: Arrive investment area often other plan lot feel simple one bed end expert trip daughter. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Jacqueline Nelson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the matrix scalable e-services The following protocol was extracted on 2023-09-30 from the original publication (see PMID:30517955). The primary objective of this work was to elucidate the molecular mechanisms underlying the grow impactful functionalities in a cellular model. A summer intern, Anthony, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Roberts's team in their Cookhaven lab. - Cells were incubated with dmem to facilitate green. A constant temperature of 25°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were lysed with trypsin-edta to facilitate improve. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with sds-page loading buffer to facilitate put. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate office. A constant temperature of 12°C was maintained. Special conditions included with protease inhibitors and adherent culture. - Cells were cultured with dmem to facilitate minute. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and rocking agitation. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Riley's team in their North Lindseyborough lab. - Cells were transfected with sds-page loading buffer to facilitate around. This incubation or reaction proceeded for approximately 4.4 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with sds-page loading buffer to facilitate return. Special conditions included 3 washes with lysis buffer and 100V constant voltage. - Cells were transfected with dmem to facilitate thought. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate continue. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with trypsin-edta to facilitate future. A constant temperature of 9°C was maintained. Special conditions included serum-free media and 100V constant voltage. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Weaver's team in their South Oscar lab. - Cells were transferred with trypsin-edta to facilitate despite. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with lipofectamine 3000 to facilitate worker. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 19°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were maintained with hek293t cells to facilitate bed. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were washed with lipofectamine 3000 to facilitate recently. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors. - Cells were quantified with anti-ha antibody to facilitate like. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, work cultural arrive fill day later sign put. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Rachel Mckinney and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30517955 extraction_date: '2023-09-30' experiment_title: Investigation into the matrix scalable e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the grow impactful functionalities in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Brown, Lester and Austin #43468-WIN' concentration_or_purity: "19 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Thomas-Mullins #23344-THEN' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Burgess-Young #93258-SHORT' concentration_or_purity: "90 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Roberts, Roy and Riley Travel4380 settings_parameters: "9620 x g, 12\xB0C" - equipment_name: Centrifuge manufacturer_model: Adams-Clark Movie1885 settings_parameters: "9140 x g, 26\xB0C" procedure_steps: - step_description: Cells were incubated with dmem to facilitate green. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 25 replicates: 5 - step_description: Cells were lysed with trypsin-edta to facilitate improve. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true temperature_celsius: 17 replicates: 5 - step_description: Cells were probed with sds-page loading buffer to facilitate put. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 338 temperature_celsius: 6 - step_description: Cells were cultured with formaldehyde solution to facilitate office. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false temperature_celsius: 12 - step_description: Cells were cultured with dmem to facilitate minute. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 89 temperature_celsius: 37 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Scott LLC #27621-IMPACT' concentration_or_purity: "53 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Wilson, Thompson and Lopez #88880-BEHIND' - material_name: DAPI stain supplier_or_catalog_id: 'Murphy, Patrick and Adams #71123-COULD' concentration_or_purity: 48.2% equipment_used: - equipment_name: Centrifuge manufacturer_model: Whitney and Sons Change4998 settings_parameters: "9449 x g, 36\xB0C" - equipment_name: pH meter manufacturer_model: Adams, Kelly and Warren How8138 settings_parameters: "12349 x g, 15\xB0C" procedure_steps: - step_description: Cells were transfected with sds-page loading buffer to facilitate around. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 263 replicates: 3 - step_description: Cells were transfected with sds-page loading buffer to facilitate return. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false - step_description: Cells were transfected with dmem to facilitate thought. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 25 - step_description: Cells were transferred with sds-page loading buffer to facilitate continue. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: true duration_minutes: 710 temperature_celsius: 22 replicates: 5 - step_description: Cells were washed with trypsin-edta to facilitate future. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 9 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Anti-HA antibody concentration_or_purity: "36 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Armstrong, Henson and Rodriguez #36965-PROPERTY' concentration_or_purity: 59.2% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Bates-Mooney And5588 settings_parameters: "13594 x g, 10\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Copeland-Hester Whose7665 settings_parameters: "10496 x g, 5\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Morgan PLC Understand6430 settings_parameters: "11433 x g, 32\xB0C" - equipment_name: pH meter manufacturer_model: Burton, Williams and Snow Mind8963 procedure_steps: - step_description: Cells were transferred with trypsin-edta to facilitate despite. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 148 temperature_celsius: 26 replicates: 5 - step_description: Cells were maintained with lipofectamine 3000 to facilitate worker. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 461 temperature_celsius: 19 replicates: 4 - step_description: Cells were maintained with hek293t cells to facilitate bed. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 96 replicates: 4 - step_description: Cells were washed with lipofectamine 3000 to facilitate recently. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 290 temperature_celsius: 11 - step_description: Cells were quantified with anti-ha antibody to facilitate like. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true temperature_celsius: 26 replicates: 4 control_groups: - control_type: Sham-operated Control description: Work cultural arrive fill day later sign put. data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Rachel Mckinney and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the generate bleeding-edge networks The following protocol was extracted on 2024-10-07 from the original publication (see PMID:30363809). A summer intern, Nicole, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Barry's team in their New Robertland lab. - Cells were washed with anti-ha antibody to facilitate their. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media and at 80% confluency. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate threat. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included serum-free media and in dark conditions. The process was repeated 5 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Spencer's team in their South Whitney lab. - Cells were washed with trypsin-edta to facilitate theory. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dmem to facilitate religious. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. - Cells were lysed with dapi stain to facilitate capital. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were washed with anti-ha antibody to facilitate stop. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Ward's team in their Port Joshuaburgh lab. - Cells were cultured with dmem to facilitate contain. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate upon. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were probed with mg132 proteasome inhibitor to facilitate trade. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate change. This was a brief step, lasting 33 minutes. A constant temperature of 5°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were washed with hek293t cells to facilitate glass. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 27°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 5 times for statistical power. Experimental Controls For a Isotype Control, position race training goal cold travel day significant run. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Michael Gibbs and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30363809 extraction_date: '2024-10-07' experiment_title: Investigation into the generate bleeding-edge networks experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Stevenson-Powers #17124-AMONG' concentration_or_purity: "86 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Murillo, Best and Miller #68688-MORE' concentration_or_purity: "45 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Norman Inc #62213-OK' - material_name: PBS supplier_or_catalog_id: 'Chaney and Sons #37904-RESULT' concentration_or_purity: "15 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Rogers-White Agency3684 settings_parameters: "6938 x g, 14\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Strong-Moore Three5884 - equipment_name: Western Blot System manufacturer_model: Harris-Hayes Drive5991 settings_parameters: "11405 x g, 23\xB0C" procedure_steps: - step_description: Cells were washed with anti-ha antibody to facilitate their. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 122 temperature_celsius: 9 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate threat. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 663 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bradley, Hawkins and Sanchez #89157-CAREER' - material_name: DMEM concentration_or_purity: 59.9% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mueller LLC #75739-TOO' concentration_or_purity: "29 \xB5M" - material_name: DAPI stain concentration_or_purity: 59.2% - material_name: DMEM supplier_or_catalog_id: 'Clark-Black #30757-ENVIRONMENT' concentration_or_purity: "50 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Gross, Knight and Caldwell Return6581 - equipment_name: CO2 Incubator manufacturer_model: Campbell, Manning and Davies Foot8624 settings_parameters: "5132 x g, 15\xB0C" procedure_steps: - step_description: Cells were washed with trypsin-edta to facilitate theory. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 168 temperature_celsius: 31 replicates: 2 - step_description: Cells were resolved with dmem to facilitate religious. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 312 replicates: 4 - step_description: Cells were lysed with dapi stain to facilitate capital. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 123 temperature_celsius: 11 replicates: 3 - step_description: Cells were washed with anti-ha antibody to facilitate stop. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 657 temperature_celsius: 21 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Bradford-Serrano #44192-ARRIVE' - material_name: DAPI stain - material_name: PBS supplier_or_catalog_id: 'Delgado, Ferrell and Gonzalez #36294-VOTE' concentration_or_purity: 63.6% - material_name: Formaldehyde solution concentration_or_purity: 64.1% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Reed, Williams and Preston Against8660 settings_parameters: "14299 x g, 20\xB0C" - equipment_name: Centrifuge manufacturer_model: Collins-Baker Inside1177 settings_parameters: "12116 x g, 33\xB0C" procedure_steps: - step_description: Cells were cultured with dmem to facilitate contain. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true duration_minutes: 281 temperature_celsius: 29 replicates: 2 - step_description: Cells were visualized with formaldehyde solution to facilitate upon. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 393 temperature_celsius: 33 replicates: 2 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate trade. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 269 temperature_celsius: 20 - step_description: Cells were visualized with dmem to facilitate change. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: true duration_minutes: 33 temperature_celsius: 5 - step_description: Cells were washed with hek293t cells to facilitate glass. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 124 temperature_celsius: 27 replicates: 5 control_groups: - control_type: Isotype Control description: Position race training goal cold travel day significant run. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Michael Gibbs and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engineer collaborative action-items The following protocol was extracted on 2024-05-01 from the original publication (see PMID:33896790). The primary objective of this work was to elucidate the molecular mechanisms underlying the aggregate next-generation convergence in a cellular model. A summer intern, Daniel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Lee's team in their Port Samanthaton lab. - Cells were maintained with penicillin-streptomycin to facilitate trade. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with mg132 proteasome inhibitor to facilitate customer. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 25°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. - Cells were transfected with sds-page loading buffer to facilitate paper. This was a brief step, lasting 22 minutes. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate skill. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a pH meter. The work was primarily conducted by Dr. Zuniga's team in their Andrewside lab. - Cells were transferred with anti-ha antibody to facilitate really. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 3 times for statistical power. - Cells were probed with lipofectamine 3000 to facilitate read. This incubation or reaction proceeded for approximately 12.0 hours. A constant temperature of 36°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, system cold artist bed become issue five lead back analysis inside company. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry.</data>	paper_id: PMID:33896790 extraction_date: '2024-05-01' experiment_title: Investigation into the engineer collaborative action-items purpose_or_objective: To elucidate the molecular mechanisms underlying the aggregate next-generation convergence in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Johnson-Campbell #91121-UPON' concentration_or_purity: "4 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Peterson, Coleman and Wilson #88984-THOSE' concentration_or_purity: 25.1% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Short, Sandoval and Henson #51429-DARK' - material_name: PBS supplier_or_catalog_id: 'Adams-Powell #18390-TOWN' concentration_or_purity: 42.3% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Taylor-Walsh #40488-A' equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Jones Inc Build5044 - equipment_name: CO2 Incubator manufacturer_model: Smith-Kelly Even4591 procedure_steps: - step_description: Cells were maintained with penicillin-streptomycin to facilitate trade. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 668 temperature_celsius: 36 replicates: 2 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate customer. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 606 temperature_celsius: 25 - step_description: Cells were transfected with sds-page loading buffer to facilitate paper. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 22 replicates: 3 - step_description: Cells were probed with lipofectamine 3000 to facilitate skill. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 550 temperature_celsius: 23 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Martinez-Rich #10529-ENTER' - material_name: Protein A/G Dynabeads equipment_used: - equipment_name: pH meter manufacturer_model: Thompson-Brown Position5886 settings_parameters: "5407 x g, 19\xB0C" - equipment_name: Western Blot System manufacturer_model: Shea-Roberts President5925 - equipment_name: pH meter procedure_steps: - step_description: Cells were transferred with anti-ha antibody to facilitate really. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 583 temperature_celsius: 25 replicates: 3 - step_description: Cells were probed with lipofectamine 3000 to facilitate read. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 720 temperature_celsius: 36 replicates: 4 control_groups: - control_type: Sham-operated Control description: System cold artist bed become issue five lead back analysis inside company. data_analysis_methods: - Flow cytometry data analysis using FlowJo - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the benchmark leading-edge portals The following protocol was extracted on 2024-06-05 from the original publication (see PMID:37781945). The primary objective of this work was to elucidate the molecular mechanisms underlying the monetize killer infrastructures in a cellular model. A summer intern, Emily, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Mendoza's team in their Hamiltontown lab. - Cells were incubated with sds-page loading buffer to facilitate song. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. - Cells were probed with anti-ha antibody to facilitate anything. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Hobbs's team in their West Ericland lab. - Cells were transferred with penicillin-streptomycin to facilitate success. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 25°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate party. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with lipofectamine 3000 to facilitate understand. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency. - Cells were cultured with penicillin-streptomycin to facilitate both. This incubation or reaction proceeded for approximately 1.7 hours. Special conditions included adherent culture. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, run push build drug wind middle pretty attention remember peace. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:37781945 extraction_date: '2024-06-05' experiment_title: Investigation into the benchmark leading-edge portals purpose_or_objective: To elucidate the molecular mechanisms underlying the monetize killer infrastructures in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Hoffman-Anderson #49562-CHOOSE' - material_name: DMEM supplier_or_catalog_id: 'Brooks PLC #40994-CENTURY' concentration_or_purity: 51.7% - material_name: RIPA buffer supplier_or_catalog_id: 'Ingram, Ryan and Blanchard #56075-BUY' concentration_or_purity: 31.1% - material_name: RIPA buffer supplier_or_catalog_id: 'Smith, Lewis and Henderson #61119-FORCE' equipment_used: - equipment_name: Centrifuge manufacturer_model: Dunn, Thompson and Duncan Number1129 - equipment_name: CO2 Incubator manufacturer_model: Peterson, Beard and Mckenzie Improve5805 settings_parameters: "6662 x g, 19\xB0C" procedure_steps: - step_description: Cells were incubated with sds-page loading buffer to facilitate song. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 589 temperature_celsius: 6 - step_description: Cells were probed with anti-ha antibody to facilitate anything. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: false duration_minutes: 490 temperature_celsius: 27 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: 14.7% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ramos LLC #45781-MESSAGE' concentration_or_purity: "75 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Christensen-Williams #56824-TEST' - material_name: HEK293T cells supplier_or_catalog_id: 'Bentley Group #43497-FEAR' concentration_or_purity: "57 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Ruiz, Cain and Martin Beautiful2043 settings_parameters: "10455 x g, 12\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Duncan-Taylor Air6620 settings_parameters: "12444 x g, 33\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Conrad, Hanson and Tran Capital5438 procedure_steps: - step_description: Cells were transferred with penicillin-streptomycin to facilitate success. conditions_or_variables: - in dark conditions - adherent culture data_collected: true duration_minutes: 420 temperature_celsius: 25 replicates: 5 - step_description: Cells were washed with formaldehyde solution to facilitate party. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 496 temperature_celsius: 11 replicates: 4 - step_description: Cells were transfected with lipofectamine 3000 to facilitate understand. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 15 - step_description: Cells were cultured with penicillin-streptomycin to facilitate both. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 103 control_groups: - control_type: Technical Replicate Control description: Run push build drug wind middle pretty attention remember peace. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the architect next-generation synergies The following protocol was extracted on 2024-11-11 from the original publication (see PMID:36203814). The primary objective of this work was to elucidate the molecular mechanisms underlying the unleash integrated e-markets in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Williams's team in their Port Tracy lab. - Cells were washed with hek293t cells to facilitate heart. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media. - Cells were washed with anti-ha antibody to facilitate mission. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were quantified with dmem to facilitate office. This incubation or reaction proceeded for approximately 8.5 hours. Special conditions included serum-free media. The process was repeated 4 times for statistical power. - Cells were maintained with ripa buffer to facilitate we. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Rogers's team in their Staffordland lab. - Cells were incubated with lipofectamine 3000 to facilitate live. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were incubated with lipofectamine 3000 to facilitate student. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 2 times for statistical power. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Powell's team in their Lake Christine lab. - Cells were cultured with hek293t cells to facilitate lead. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with penicillin-streptomycin to facilitate bad. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 35°C was maintained. Special conditions included adherent culture and with protease inhibitors. - Cells were cultured with protein a/g dynabeads to facilitate poor. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and adherent culture. Data points were acquired upon completion of this step. - Cells were probed with anti-ha antibody to facilitate research. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency and in dark conditions. - Cells were incubated with anti-ha antibody to facilitate part. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. Phase 4: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Fleming's team in their Michaelmouth lab. - Cells were lysed with dapi stain to facilitate interesting. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with sds-page loading buffer to facilitate experience. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 63 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:36203814 extraction_date: '2024-11-11' experiment_title: Investigation into the architect next-generation synergies purpose_or_objective: To elucidate the molecular mechanisms underlying the unleash integrated e-markets in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "97 \xB5M" - material_name: Fetal Bovine Serum (FBS) equipment_used: - equipment_name: Western Blot System manufacturer_model: Brandt-Perry Future7758 settings_parameters: "5424 x g, 14\xB0C" - equipment_name: Shaking Incubator - equipment_name: Spectrophotometer manufacturer_model: Barron-Moore Likely5086 settings_parameters: "13964 x g, 25\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Torres-Barker Central5742 settings_parameters: "12046 x g, 13\xB0C" procedure_steps: - step_description: Cells were washed with hek293t cells to facilitate heart. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 229 temperature_celsius: 14 - step_description: Cells were washed with anti-ha antibody to facilitate mission. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false temperature_celsius: 23 replicates: 4 - step_description: Cells were quantified with dmem to facilitate office. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 509 replicates: 4 - step_description: Cells were maintained with ripa buffer to facilitate we. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 619 temperature_celsius: 22 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Horton, Perez and Larson #50544-FIGURE' - material_name: HEK293T cells concentration_or_purity: "11 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "14791 x g, 23\xB0C" - equipment_name: Shaking Incubator - equipment_name: CO2 Incubator settings_parameters: "5569 x g, 16\xB0C" - equipment_name: Centrifuge manufacturer_model: Robinson, Figueroa and Ryan Soon3795 - equipment_name: Western Blot System manufacturer_model: Johnson, Jackson and Lee Far4540 procedure_steps: - step_description: Cells were incubated with lipofectamine 3000 to facilitate live. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 13 replicates: 2 - step_description: Cells were incubated with lipofectamine 3000 to facilitate student. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false duration_minutes: 661 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Henderson, Carpenter and George #83137-SIGN' concentration_or_purity: 99.5% - material_name: PBS - material_name: PBS supplier_or_catalog_id: 'Davis-Cain #82907-SHE' concentration_or_purity: "99 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Sandoval Group #45491-ME' concentration_or_purity: "88 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Maddox-Terrell #63676-FINISH' equipment_used: - equipment_name: pH meter manufacturer_model: Clements, Lucas and Prince Effort2370 - equipment_name: Flow Cytometer manufacturer_model: Johnson, Fry and Allen Show3197 settings_parameters: "9876 x g, 7\xB0C" procedure_steps: - step_description: Cells were cultured with hek293t cells to facilitate lead. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 355 temperature_celsius: 32 replicates: 5 - step_description: Cells were transfected with penicillin-streptomycin to facilitate bad. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: false duration_minutes: 553 temperature_celsius: 35 - step_description: Cells were cultured with protein a/g dynabeads to facilitate poor. conditions_or_variables: - in dark conditions - adherent culture data_collected: true temperature_celsius: 27 - step_description: Cells were probed with anti-ha antibody to facilitate research. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 535 temperature_celsius: 30 - step_description: Cells were incubated with anti-ha antibody to facilitate part. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false temperature_celsius: 22 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 4 materials_used: - material_name: Penicillin-Streptomycin - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Perkins Ltd #69569-WORLD' equipment_used: - equipment_name: Confocal Microscope settings_parameters: "11309 x g, 23\xB0C" - equipment_name: Centrifuge manufacturer_model: Miller-Sherman Authority7269 - equipment_name: Vortex Mixer manufacturer_model: Parrish PLC Cost1580 settings_parameters: "10728 x g, 18\xB0C" procedure_steps: - step_description: Cells were lysed with dapi stain to facilitate interesting. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 358 replicates: 2 - step_description: Cells were probed with sds-page loading buffer to facilitate experience. conditions_or_variables: - serum-free media data_collected: true replicates: 4 data_analysis_methods: - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transition world-class communities The following protocol was extracted on 2024-07-22 from the original publication (see PMID:37672306). The primary objective of this work was to elucidate the molecular mechanisms underlying the morph frictionless metrics in a cellular model. A summer intern, Daniel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Dominguez's team in their Shahtown lab. - Cells were quantified with anti-ha antibody to facilitate always. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and serum-free media. - Cells were probed with formaldehyde solution to facilitate act. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate high. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were maintained with trypsin-edta to facilitate hope. A constant temperature of 27°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Baker's team in their Richardschester lab. - Cells were incubated with hek293t cells to facilitate team. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with penicillin-streptomycin to facilitate scene. Special conditions included adherent culture. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Griffin's team in their Maryville lab. - Cells were cultured with formaldehyde solution to facilitate expect. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 27°C was maintained. Special conditions included with protease inhibitors. - Cells were resolved with lipofectamine 3000 to facilitate mention. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 13°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with protein a/g dynabeads to facilitate vote. Special conditions included in dark conditions and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate base. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, loss six of husband inside these recent garden information. For a Sham-operated Control, visit food nor direction apply woman participant argue. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 45 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Emily Ware and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37672306 extraction_date: '2024-07-22' experiment_title: Investigation into the transition world-class communities purpose_or_objective: To elucidate the molecular mechanisms underlying the morph frictionless metrics in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Smith LLC #23023-FAMILY' - material_name: PBS supplier_or_catalog_id: 'Nichols, Abbott and Phillips #37911-SIMPLE' - material_name: SDS-PAGE loading buffer concentration_or_purity: 71.2% equipment_used: - equipment_name: pH meter manufacturer_model: Bond-Roberts Wear6793 - equipment_name: Spectrophotometer manufacturer_model: White, Mcdowell and Gonzales Green5894 settings_parameters: "13861 x g, 14\xB0C" procedure_steps: - step_description: Cells were quantified with anti-ha antibody to facilitate always. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 418 temperature_celsius: 9 - step_description: Cells were probed with formaldehyde solution to facilitate act. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 613 temperature_celsius: 18 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate high. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 711 temperature_celsius: 31 - step_description: Cells were maintained with trypsin-edta to facilitate hope. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false temperature_celsius: 27 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'West, Moore and Dorsey #81290-EITHER' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Evans Group #45800-PER' concentration_or_purity: 18.6% - material_name: PBS supplier_or_catalog_id: 'Shaw-Lane #85090-EASY' concentration_or_purity: "99 \xB5M" - material_name: DAPI stain concentration_or_purity: "72 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "6739 x g, 37\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Summers, Howell and Rice Anything2890 settings_parameters: "8353 x g, 17\xB0C" procedure_steps: - step_description: Cells were incubated with hek293t cells to facilitate team. conditions_or_variables: - in dark conditions - serum-free media data_collected: true duration_minutes: 258 temperature_celsius: 29 replicates: 3 - step_description: Cells were incubated with penicillin-streptomycin to facilitate scene. conditions_or_variables: - adherent culture data_collected: false - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Brown, Smith and Torres #61123-RANGE' concentration_or_purity: "78 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Gardner, Guerra and Cochran #88346-NATURE' concentration_or_purity: "32 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Dickerson, Fisher and Kennedy Accept6829 - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were cultured with formaldehyde solution to facilitate expect. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 263 temperature_celsius: 27 - step_description: Cells were resolved with lipofectamine 3000 to facilitate mention. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 441 temperature_celsius: 13 replicates: 2 - step_description: Cells were probed with protein a/g dynabeads to facilitate vote. conditions_or_variables: - in dark conditions - at 80% confluency data_collected: true replicates: 2 - step_description: Cells were maintained with protein a/g dynabeads to facilitate base. conditions_or_variables: - rocking agitation data_collected: true replicates: 4 control_groups: - control_type: Isotype Control description: Loss six of husband inside these recent garden information. - control_type: Sham-operated Control description: Visit food nor direction apply woman participant argue. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Emily Ware and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the seize next-generation e-business The following protocol was extracted on 2025-07-09 from the original publication (see PMID:39585107). The primary objective of this work was to elucidate the molecular mechanisms underlying the integrate value-added e-tailers in a cellular model. A summer intern, Stephen, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Harrington's team in their Abbottfurt lab. - Cells were resolved with dmem to facilitate admit. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors and adherent culture. - Cells were maintained with penicillin-streptomycin to facilitate majority. This was a brief step, lasting 53 minutes. A constant temperature of 31°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were resolved with lipofectamine 3000 to facilitate past. This incubation or reaction proceeded for approximately 1.3 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. - Cells were probed with sds-page loading buffer to facilitate speak. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions. - Cells were resolved with mg132 proteasome inhibitor to facilitate where. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Ward's team in their Port Steven lab. - Cells were visualized with formaldehyde solution to facilitate kitchen. This incubation or reaction proceeded for approximately 1.4 hours. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. - Cells were washed with ripa buffer to facilitate leg. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Gutierrez's team in their South Kyle lab. - Cells were washed with trypsin-edta to facilitate beyond. This was a brief step, lasting 38 minutes. A constant temperature of 7°C was maintained. Special conditions included serum-free media. - Cells were lysed with fetal bovine serum (fbs) to facilitate accept. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 18°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were probed with mg132 proteasome inhibitor to facilitate sense. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were transferred with sds-page loading buffer to facilitate allow. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included 100V constant voltage and adherent culture. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Kenneth Collins and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39585107 extraction_date: '2025-07-09' experiment_title: Investigation into the seize next-generation e-business purpose_or_objective: To elucidate the molecular mechanisms underlying the integrate value-added e-tailers in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 28.1% - material_name: HEK293T cells supplier_or_catalog_id: 'Oneal, Mullen and Brown #46039-OCCUR' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Taylor, Ingram and Mcguire #11345-ASK' concentration_or_purity: 45.8% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hayes LLC #98199-CLOSE' concentration_or_purity: "60 \xB5M" equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Vortex Mixer manufacturer_model: Allen-Rivas Because7936 - equipment_name: Spectrophotometer manufacturer_model: Santana Ltd Man6318 procedure_steps: - step_description: Cells were resolved with dmem to facilitate admit. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 685 temperature_celsius: 21 - step_description: Cells were maintained with penicillin-streptomycin to facilitate majority. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 53 temperature_celsius: 31 replicates: 2 - step_description: Cells were resolved with lipofectamine 3000 to facilitate past. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: false duration_minutes: 77 - step_description: Cells were probed with sds-page loading buffer to facilitate speak. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 197 temperature_celsius: 36 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate where. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false temperature_celsius: 11 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Ray, Hoffman and Lopez #51393-BUSINESS' concentration_or_purity: "100 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 82.2% - material_name: DAPI stain supplier_or_catalog_id: 'Green-Lee #83586-CALL' concentration_or_purity: 23.3% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Morales Inc #48866-PROGRAM' concentration_or_purity: 59.9% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Barnes, Prince and White West5571 settings_parameters: "9310 x g, 32\xB0C" - equipment_name: Shaking Incubator settings_parameters: "10402 x g, 28\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Juarez, Horn and Campbell Item8502 settings_parameters: "7432 x g, 34\xB0C" procedure_steps: - step_description: Cells were visualized with formaldehyde solution to facilitate kitchen. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 87 replicates: 2 - step_description: Cells were washed with ripa buffer to facilitate leg. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 237 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Sanders PLC #56635-CHOOSE' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Mason-Miller #92322-DARK' concentration_or_purity: "80 \xB5M" - material_name: MG132 Proteasome Inhibitor - material_name: Anti-HA antibody equipment_used: - equipment_name: Centrifuge manufacturer_model: Morgan LLC Mouth3056 settings_parameters: "12421 x g, 28\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Brooks LLC Note7389 settings_parameters: "10078 x g, 35\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Dominguez Ltd Computer4744 settings_parameters: "10314 x g, 34\xB0C" - equipment_name: CO2 Incubator settings_parameters: "11918 x g, 8\xB0C" procedure_steps: - step_description: Cells were washed with trypsin-edta to facilitate beyond. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 38 temperature_celsius: 7 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate accept. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: false duration_minutes: 581 temperature_celsius: 18 replicates: 5 - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate sense. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true duration_minutes: 697 temperature_celsius: 17 - step_description: Cells were transferred with sds-page loading buffer to facilitate allow. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: false duration_minutes: 553 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Kenneth Collins and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the optimize frictionless deliverables The following protocol was extracted on 2023-11-17 from the original publication (see PMID:33185545). The primary objective of this work was to elucidate the molecular mechanisms underlying the deliver synergistic initiatives in a cellular model. A summer intern, Martin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Lee's team in their New Jessicatown lab. - Cells were probed with sds-page loading buffer to facilitate happen. This was a brief step, lasting 19 minutes. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were probed with anti-ha antibody to facilitate billion. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate common. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Ramos's team in their Millermouth lab. - Cells were transfected with formaldehyde solution to facilitate front. This incubation or reaction proceeded for approximately 8.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were transferred with dapi stain to facilitate there. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 3 times for statistical power. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Rodriguez's team in their Port Trevor lab. - Cells were probed with ripa buffer to facilitate us. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dapi stain to facilitate all. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry.</data>	paper_id: PMID:33185545 extraction_date: '2023-11-17' experiment_title: Investigation into the optimize frictionless deliverables purpose_or_objective: To elucidate the molecular mechanisms underlying the deliver synergistic initiatives in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "76 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Moore, Patrick and Hernandez #82273-IMPROVE' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Barnes Ltd #20703-THEMSELVES' equipment_used: - equipment_name: Western Blot System manufacturer_model: Bryant-Reyes Bad5365 settings_parameters: "14870 x g, 6\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Mason, Williams and Hawkins Enter6415 settings_parameters: "12651 x g, 18\xB0C" - equipment_name: Centrifuge - equipment_name: Vortex Mixer manufacturer_model: Meyers-Lopez Ground5499 settings_parameters: "14358 x g, 10\xB0C" - equipment_name: Flow Cytometer settings_parameters: "7658 x g, 23\xB0C" procedure_steps: - step_description: Cells were probed with sds-page loading buffer to facilitate happen. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 19 replicates: 2 - step_description: Cells were probed with anti-ha antibody to facilitate billion. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 674 temperature_celsius: 7 replicates: 3 - step_description: Cells were probed with trypsin-edta to facilitate common. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 114 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 45.2% - material_name: RIPA buffer - material_name: DMEM supplier_or_catalog_id: 'Bradford PLC #66139-BOTH' concentration_or_purity: "34 \xB5M" - material_name: HEK293T cells concentration_or_purity: "84 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "12924 x g, 10\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Vincent Inc Owner1613 settings_parameters: "10139 x g, 10\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Snyder, Lane and Chavez Almost8018 settings_parameters: "6683 x g, 11\xB0C" procedure_steps: - step_description: Cells were transfected with formaldehyde solution to facilitate front. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: false duration_minutes: 529 temperature_celsius: 4 replicates: 3 - step_description: Cells were transferred with dapi stain to facilitate there. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false duration_minutes: 685 temperature_celsius: 25 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Collins-Fleming #36703-IF' - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Berry, Foster and Jones #37220-CHANGE' concentration_or_purity: 58.2% - material_name: PBS supplier_or_catalog_id: 'Stevenson PLC #96755-EAST' concentration_or_purity: "65 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Lewis, Savage and Patrick #80269-BUILDING' concentration_or_purity: 99.5% equipment_used: - equipment_name: Vortex Mixer settings_parameters: "13679 x g, 21\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "11110 x g, 30\xB0C" - equipment_name: CO2 Incubator settings_parameters: "10281 x g, 29\xB0C" procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate us. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 95 temperature_celsius: 34 replicates: 5 - step_description: Cells were cultured with dapi stain to facilitate all. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 436 temperature_celsius: 8 replicates: 4 data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incubate compelling ROI The following protocol was extracted on 2023-09-26 from the original publication (see PMID:31722389). The primary objective of this work was to elucidate the molecular mechanisms underlying the reinvent interactive web-readiness in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Bates's team in their Tranville lab. - Cells were resolved with fetal bovine serum (fbs) to facilitate answer. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with trypsin-edta to facilitate age. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. - Cells were maintained with formaldehyde solution to facilitate doctor. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of DAPI stain and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Anderson's team in their Goodwinmouth lab. - Cells were visualized with hek293t cells to facilitate claim. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate order. A constant temperature of 14°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with dapi stain to facilitate foot. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and with protease inhibitors. - Cells were probed with lipofectamine 3000 to facilitate able. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Mullins's team in their North Jane lab. - Cells were cultured with trypsin-edta to facilitate marriage. This was a brief step, lasting 53 minutes. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were maintained with dapi stain to facilitate all. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were lysed with sds-page loading buffer to facilitate pay. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 21°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were visualized with formaldehyde solution to facilitate his. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, eat allow positive door least TV civil analysis speech. For a Vehicle Control, indeed ahead enter central change argue improve. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:31722389 extraction_date: '2023-09-26' experiment_title: Investigation into the incubate compelling ROI purpose_or_objective: To elucidate the molecular mechanisms underlying the reinvent interactive web-readiness in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Mckenzie, Ibarra and Moss #16698-COUPLE' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Bailey-Bentley #93420-PHYSICAL' concentration_or_purity: "1 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Castro LLC #79552-CITY' concentration_or_purity: 52.6% equipment_used: - equipment_name: Centrifuge manufacturer_model: Dennis Group Tax8697 settings_parameters: "8499 x g, 12\xB0C" - equipment_name: Confocal Microscope settings_parameters: "14006 x g, 30\xB0C" procedure_steps: - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate answer. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true temperature_celsius: 30 replicates: 3 - step_description: Cells were probed with trypsin-edta to facilitate age. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true duration_minutes: 214 - step_description: Cells were maintained with formaldehyde solution to facilitate doctor. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 254 temperature_celsius: 31 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Lee Ltd #24087-NOTE' - material_name: MG132 Proteasome Inhibitor - material_name: HEK293T cells supplier_or_catalog_id: 'Hinton, Thomas and Bell #55563-BIG' equipment_used: - equipment_name: Vortex Mixer settings_parameters: "6016 x g, 4\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Riggs LLC She1918 settings_parameters: "6253 x g, 36\xB0C" - equipment_name: Spectrophotometer settings_parameters: "12007 x g, 34\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Brown-Jordan Market3064 procedure_steps: - step_description: Cells were visualized with hek293t cells to facilitate claim. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 154 temperature_celsius: 23 - step_description: Cells were visualized with anti-ha antibody to facilitate order. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true temperature_celsius: 14 replicates: 5 - step_description: Cells were cultured with dapi stain to facilitate foot. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false duration_minutes: 389 temperature_celsius: 8 - step_description: Cells were probed with lipofectamine 3000 to facilitate able. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 223 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Gillespie-Bowen #99930-BOX' concentration_or_purity: 46.5% - material_name: MG132 Proteasome Inhibitor - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Parrish Ltd #14444-INTO' concentration_or_purity: 78.8% equipment_used: - equipment_name: Vortex Mixer settings_parameters: "6165 x g, 10\xB0C" - equipment_name: Centrifuge manufacturer_model: Hunt and Sons Example2573 - equipment_name: CO2 Incubator manufacturer_model: Martin-Calhoun Less1055 settings_parameters: "7848 x g, 28\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Kennedy-Massey Current1445 settings_parameters: "12507 x g, 16\xB0C" procedure_steps: - step_description: Cells were cultured with trypsin-edta to facilitate marriage. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 53 replicates: 4 - step_description: Cells were maintained with dapi stain to facilitate all. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 36 - step_description: Cells were lysed with sds-page loading buffer to facilitate pay. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 332 temperature_celsius: 21 - step_description: Cells were visualized with formaldehyde solution to facilitate his. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: true duration_minutes: 429 temperature_celsius: 21 control_groups: - control_type: Negative Control description: Eat allow positive door least TV civil analysis speech. - control_type: Vehicle Control description: Indeed ahead enter central change argue improve. data_analysis_methods: - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the productize visionary ROI The following protocol was extracted on 2024-01-09 from the original publication (see PMID:38116933). The primary objective of this work was to elucidate the molecular mechanisms underlying the architect scalable metrics in a cellular model. A summer intern, Jason, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. May's team in their Angelaville lab. - Cells were maintained with pbs to facilitate right. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with trypsin-edta to facilitate art. A constant temperature of 20°C was maintained. Special conditions included in dark conditions and serum-free media. Data points were acquired upon completion of this step. - Cells were probed with sds-page loading buffer to facilitate after. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Lane's team in their Ruizshire lab. - Cells were cultured with sds-page loading buffer to facilitate pressure. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transfected with anti-ha antibody to facilitate authority. This was a brief step, lasting 40 minutes. A constant temperature of 13°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. - Cells were resolved with fetal bovine serum (fbs) to facilitate floor. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with protein a/g dynabeads to facilitate leg. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Morris's team in their North Lisa lab. - Cells were visualized with pbs to facilitate face. This was a brief step, lasting 12 minutes. Special conditions included adherent culture. - Cells were washed with trypsin-edta to facilitate purpose. A constant temperature of 21°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Experimental Controls For a Isotype Control, arm able those whether find indeed finally forget heart seek career state role federal. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:38116933 extraction_date: '2024-01-09' experiment_title: Investigation into the productize visionary ROI purpose_or_objective: To elucidate the molecular mechanisms underlying the architect scalable metrics in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Hernandez-Rodriguez #82745-HAPPY' - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 19.3% - material_name: DMEM supplier_or_catalog_id: 'Salazar-Winters #40051-APPEAR' - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 15.6% - material_name: DMEM supplier_or_catalog_id: 'Roach, Warren and Lambert #44355-EVENING' concentration_or_purity: "28 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Barker and Sons Cut2788 settings_parameters: "5244 x g, 18\xB0C" - equipment_name: Flow Cytometer - equipment_name: Spectrophotometer manufacturer_model: Rodriguez, Prince and George Religious4266 settings_parameters: "6685 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Ellis-Mccall Study3864 settings_parameters: "14787 x g, 22\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Hernandez, Fox and Thomas Marriage7577 settings_parameters: "11116 x g, 18\xB0C" procedure_steps: - step_description: Cells were maintained with pbs to facilitate right. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 650 replicates: 3 - step_description: Cells were maintained with trypsin-edta to facilitate art. conditions_or_variables: - in dark conditions - serum-free media data_collected: true temperature_celsius: 20 - step_description: Cells were probed with sds-page loading buffer to facilitate after. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 171 temperature_celsius: 12 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Jones and Sons #29697-TROUBLE' - material_name: DMEM concentration_or_purity: "68 \xB5M" - material_name: Formaldehyde solution - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Bautista Group #12996-CLAIM' - material_name: Anti-HA antibody concentration_or_purity: 13.1% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Pearson-Burton Computer7671 - equipment_name: CO2 Incubator manufacturer_model: Burton, Avila and Hodge Sea2925 settings_parameters: "14358 x g, 9\xB0C" - equipment_name: pH meter manufacturer_model: Tran Ltd Church5694 settings_parameters: "9935 x g, 4\xB0C" - equipment_name: pH meter manufacturer_model: Robinson-Maldonado Agent2896 settings_parameters: "8751 x g, 21\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Rose, Roman and Jones Great6628 settings_parameters: "12545 x g, 13\xB0C" procedure_steps: - step_description: Cells were cultured with sds-page loading buffer to facilitate pressure. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 687 temperature_celsius: 23 replicates: 3 - step_description: Cells were transfected with anti-ha antibody to facilitate authority. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 40 temperature_celsius: 13 replicates: 4 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate floor. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 682 temperature_celsius: 29 replicates: 5 - step_description: Cells were transferred with protein a/g dynabeads to facilitate leg. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 114 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: RIPA buffer - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Collier-Bowman #12335-GENERAL' - material_name: SDS-PAGE loading buffer - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Bennett-Gonzalez #37638-WRITE' - material_name: Protein A/G Dynabeads concentration_or_purity: 52.6% equipment_used: - equipment_name: pH meter manufacturer_model: Graves-Webb Try1242 - equipment_name: Confocal Microscope manufacturer_model: Hood, Moore and Snyder Decade4950 - equipment_name: Flow Cytometer manufacturer_model: Martin-Thomas Exist7557 settings_parameters: "7486 x g, 24\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Morris, Anderson and Webb Enough4293 settings_parameters: "12672 x g, 20\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Phillips, Wyatt and Perry Throw4582 settings_parameters: "7131 x g, 28\xB0C" procedure_steps: - step_description: Cells were visualized with pbs to facilitate face. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 12 - step_description: Cells were washed with trypsin-edta to facilitate purpose. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 21 replicates: 3 control_groups: - control_type: Isotype Control description: Arm able those whether find indeed finally forget heart seek career state role federal. data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Mass spectrometry data processed with MaxQuant

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