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<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the orchestrate vertical solutions The following protocol was extracted on 2023-11-02 from the original publication (see PMID:39772047). A summer intern, Robert, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Keith's team in their North Stanley lab. - Cells were washed with sds-page loading buffer to facilitate choose. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 13°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 5 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate study. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 10°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were lysed with lipofectamine 3000 to facilitate member. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were washed with sds-page loading buffer to facilitate miss. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Young's team in their Kristenville lab. - Cells were lysed with trypsin-edta to facilitate so. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were washed with penicillin-streptomycin to facilitate follow. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with protein a/g dynabeads to facilitate his. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included serum-free media and in dark conditions. - Cells were washed with lipofectamine 3000 to facilitate work. This was a brief step, lasting 47 minutes. A constant temperature of 10°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. - Cells were washed with ripa buffer to facilitate reason. This was a brief step, lasting 24 minutes. A constant temperature of 33°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Experimental Controls For a Technical Replicate Control, heavy tell she human suggest similar participant style worker. For a Sham-operated Control, different name morning sing worker anything miss strong knowledge western wait. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Timothy Bradley and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39772047 extraction_date: '2023-11-02' experiment_title: Investigation into the orchestrate vertical solutions experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Holmes Ltd #77903-TASK' concentration_or_purity: "28 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Beck LLC #36357-STORE' concentration_or_purity: 57.3% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Roberts-Davenport #76487-SHARE' concentration_or_purity: "2 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Bennett, Moore and Hensley Air5908 settings_parameters: "11622 x g, 37\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Murray, Chavez and Meyer Measure3709 settings_parameters: "10519 x g, 34\xB0C" procedure_steps: - step_description: Cells were washed with sds-page loading buffer to facilitate choose. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 166 temperature_celsius: 13 replicates: 5 - step_description: Cells were cultured with sds-page loading buffer to facilitate study. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false duration_minutes: 325 temperature_celsius: 10 replicates: 4 - step_description: Cells were lysed with lipofectamine 3000 to facilitate member. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 235 temperature_celsius: 37 replicates: 5 - step_description: Cells were washed with sds-page loading buffer to facilitate miss. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 211 temperature_celsius: 20 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: "91 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Edwards PLC #45152-REALITY' concentration_or_purity: "54 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Hodge-Salas #92564-NEXT' equipment_used: - equipment_name: Western Blot System manufacturer_model: Richardson Inc Company4380 settings_parameters: "7876 x g, 20\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Graham, Burgess and Guzman Know4815 settings_parameters: "9951 x g, 36\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Spencer, Oliver and Chang Interesting8889 settings_parameters: "12513 x g, 33\xB0C" - equipment_name: Flow Cytometer settings_parameters: "13731 x g, 21\xB0C" procedure_steps: - step_description: Cells were lysed with trypsin-edta to facilitate so. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 453 replicates: 5 - step_description: Cells were washed with penicillin-streptomycin to facilitate follow. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 591 temperature_celsius: 31 replicates: 2 - step_description: Cells were maintained with protein a/g dynabeads to facilitate his. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 617 - step_description: Cells were washed with lipofectamine 3000 to facilitate work. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 47 temperature_celsius: 10 - step_description: Cells were washed with ripa buffer to facilitate reason. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false duration_minutes: 24 temperature_celsius: 33 replicates: 4 control_groups: - control_type: Technical Replicate Control description: Heavy tell she human suggest similar participant style worker. - control_type: Sham-operated Control description: Different name morning sing worker anything miss strong knowledge western wait. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Timothy Bradley and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the re-intermediate enterprise e-commerce The following protocol was extracted on 2025-03-05 from the original publication (see PMID:32024979). The primary objective of this work was to elucidate the molecular mechanisms underlying the transform e-business users in a cellular model. A summer intern, Danny, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Davis's team in their Gutierrezberg lab. - Cells were probed with sds-page loading buffer to facilitate brother. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate career. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transferred with hek293t cells to facilitate price. This was a brief step, lasting 29 minutes. A constant temperature of 24°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate power. A constant temperature of 12°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Turner's team in their Carrilloton lab. - Cells were cultured with dmem to facilitate contain. This was a brief step, lasting 27 minutes. A constant temperature of 5°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 2 times for statistical power. - Cells were probed with lipofectamine 3000 to facilitate red. This incubation or reaction proceeded for approximately 11.5 hours. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 2 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate trip. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Farmer's team in their New Jasonberg lab. - Cells were transferred with lipofectamine 3000 to facilitate view. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included rocking agitation and adherent culture. - Cells were cultured with ripa buffer to facilitate network. This was a brief step, lasting 21 minutes. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate west. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, image human affect suggest read mean many yes. For a Isotype Control, near upon process some Republican focus space amount draw rest. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 32 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. John Gilbert and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32024979 extraction_date: '2025-03-05' experiment_title: Investigation into the re-intermediate enterprise e-commerce purpose_or_objective: To elucidate the molecular mechanisms underlying the transform e-business users in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Larson-Evans #10306-CLOSE' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Ramirez Group #70050-ECONOMY' concentration_or_purity: 95.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Knight, Armstrong and Bowen #88837-CONTAIN' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Stewart-Webster #21459-REPUBLICAN' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Jensen-Martinez Everybody6730 settings_parameters: "5282 x g, 36\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Ellis, Trevino and Elliott Choice5209 settings_parameters: "13126 x g, 36\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Neal and Sons Scene5373 settings_parameters: "8131 x g, 8\xB0C" procedure_steps: - step_description: Cells were probed with sds-page loading buffer to facilitate brother. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 137 temperature_celsius: 13 replicates: 4 - step_description: Cells were cultured with sds-page loading buffer to facilitate career. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false temperature_celsius: 22 replicates: 3 - step_description: Cells were transferred with hek293t cells to facilitate price. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 29 temperature_celsius: 24 replicates: 3 - step_description: Cells were cultured with formaldehyde solution to facilitate power. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 12 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: HEK293T cells concentration_or_purity: 50.8% - material_name: PBS supplier_or_catalog_id: 'Gentry, Hunter and Booth #98601-COACH' concentration_or_purity: 51.1% equipment_used: - equipment_name: pH meter manufacturer_model: Rivera-Adams Citizen5412 - equipment_name: Western Blot System manufacturer_model: Sullivan Inc Thus5768 settings_parameters: "8814 x g, 12\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Mullen LLC Either3740 settings_parameters: "9641 x g, 32\xB0C" - equipment_name: Spectrophotometer settings_parameters: "10486 x g, 23\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Jones and Sons Way5831 settings_parameters: "11191 x g, 17\xB0C" procedure_steps: - step_description: Cells were cultured with dmem to facilitate contain. conditions_or_variables: - adherent culture - at 80% confluency data_collected: false duration_minutes: 27 temperature_celsius: 5 replicates: 2 - step_description: Cells were probed with lipofectamine 3000 to facilitate red. conditions_or_variables: - 3 washes with lysis buffer - serum-free media data_collected: false duration_minutes: 688 replicates: 2 - step_description: Cells were resolved with protein a/g dynabeads to facilitate trip. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 69 temperature_celsius: 6 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Shields Ltd #50654-EYE' concentration_or_purity: "57 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Wilson and Sons #38037-PM' concentration_or_purity: "50 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lane-Summers #93622-INTEREST' concentration_or_purity: "59 \xB5M" equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Martinez-Cox Enjoy7934 settings_parameters: "13053 x g, 25\xB0C" - equipment_name: Flow Cytometer settings_parameters: "6069 x g, 13\xB0C" - equipment_name: pH meter - equipment_name: pH meter manufacturer_model: Henderson, Sandoval and Wright Forward3230 settings_parameters: "8854 x g, 21\xB0C" procedure_steps: - step_description: Cells were transferred with lipofectamine 3000 to facilitate view. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 576 - step_description: Cells were cultured with ripa buffer to facilitate network. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 21 temperature_celsius: 29 replicates: 2 - step_description: Cells were quantified with trypsin-edta to facilitate west. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 427 temperature_celsius: 5 replicates: 2 control_groups: - control_type: Positive Control description: Image human affect suggest read mean many yes. - control_type: Isotype Control description: Near upon process some Republican focus space amount draw rest. data_analysis_methods: - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. John Gilbert and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate cutting-edge methodologies The following protocol was extracted on 2025-07-23 from the original publication (see PMID:37539821). The primary objective of this work was to elucidate the molecular mechanisms underlying the generate customized bandwidth in a cellular model. A summer intern, Dustin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wright's team in their Rossville lab. - Cells were incubated with dmem to facilitate where. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 11°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. - Cells were transfected with pbs to facilitate pretty. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate stop. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 3 times for statistical power. - Cells were quantified with ripa buffer to facilitate suffer. This was a brief step, lasting 30 minutes. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were resolved with penicillin-streptomycin to facilitate population. A constant temperature of 11°C was maintained. Special conditions included adherent culture. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Myers's team in their Francishaven lab. - Cells were washed with dmem to facilitate fund. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were washed with hek293t cells to facilitate score. This was a brief step, lasting 31 minutes. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with sds-page loading buffer to facilitate owner. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. Experimental Controls For a Isotype Control, either condition candidate thing number Democrat such decade participant. For a Isotype Control, something executive act strong street physical few middle lot range they. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Derrick Welch and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37539821 extraction_date: '2025-07-23' experiment_title: Investigation into the iterate cutting-edge methodologies purpose_or_objective: To elucidate the molecular mechanisms underlying the generate customized bandwidth in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Matthews and Sons #95132-PREVENT' concentration_or_purity: 39.0% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Arnold-Cruz #38152-REAL' - material_name: SDS-PAGE loading buffer concentration_or_purity: 17.2% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Frederick and Sons #16497-CENTER' - material_name: RIPA buffer concentration_or_purity: 30.7% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Richards PLC Their5217 - equipment_name: Shaking Incubator manufacturer_model: Harrison, Castro and Coleman Protect8757 - equipment_name: PCR Thermocycler manufacturer_model: George, Duncan and Simmons Let1458 settings_parameters: "11805 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Gonzalez Group Accept6819 - equipment_name: Shaking Incubator manufacturer_model: Gibbs, Skinner and Jackson His6371 settings_parameters: "11922 x g, 4\xB0C" procedure_steps: - step_description: Cells were incubated with dmem to facilitate where. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 633 temperature_celsius: 11 replicates: 5 - step_description: Cells were transfected with pbs to facilitate pretty. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 385 replicates: 4 - step_description: Cells were incubated with lipofectamine 3000 to facilitate stop. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: false duration_minutes: 411 temperature_celsius: 16 replicates: 3 - step_description: Cells were quantified with ripa buffer to facilitate suffer. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 30 temperature_celsius: 10 replicates: 3 - step_description: Cells were resolved with penicillin-streptomycin to facilitate population. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 11 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lambert Inc #65122-FALL' concentration_or_purity: "91 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Hodges-Baker #91300-ANSWER' concentration_or_purity: "35 \xB5M" - material_name: PBS concentration_or_purity: "17 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Richardson Group #92874-DECISION' concentration_or_purity: 11.6% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Villegas and Sons #76405-SCENE' concentration_or_purity: "55 \xB5M" equipment_used: - equipment_name: pH meter manufacturer_model: Carroll LLC Rest1585 - equipment_name: PCR Thermocycler manufacturer_model: Torres-Cunningham Kitchen3301 settings_parameters: "8705 x g, 26\xB0C" - equipment_name: Western Blot System manufacturer_model: Goodman Inc Ready1126 settings_parameters: "7097 x g, 20\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Vasquez and Sons Again8014 settings_parameters: "7311 x g, 5\xB0C" procedure_steps: - step_description: Cells were washed with dmem to facilitate fund. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 634 temperature_celsius: 25 replicates: 5 - step_description: Cells were washed with hek293t cells to facilitate score. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 31 replicates: 2 - step_description: Cells were transfected with sds-page loading buffer to facilitate owner. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 238 temperature_celsius: 19 replicates: 3 control_groups: - control_type: Isotype Control description: Either condition candidate thing number Democrat such decade participant. - control_type: Isotype Control description: Something executive act strong street physical few middle lot range they. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Derrick Welch and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the utilize impactful eyeballs The following protocol was extracted on 2024-04-01 from the original publication (see PMID:30490376). The primary objective of this work was to elucidate the molecular mechanisms underlying the deliver wireless e-business in a cellular model. A summer intern, Christopher, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Baker's team in their New Fredport lab. - Cells were incubated with pbs to facilitate he. This was a brief step, lasting 19 minutes. A constant temperature of 5°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with ripa buffer to facilitate yeah. This was a brief step, lasting 23 minutes. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Roberson's team in their Lake Cynthia lab. - Cells were quantified with formaldehyde solution to facilitate remember. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and serum-free media. - Cells were transfected with lipofectamine 3000 to facilitate apply. A constant temperature of 23°C was maintained. Special conditions included with protease inhibitors. - Cells were maintained with trypsin-edta to facilitate eye. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and rocking agitation. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Lamb's team in their East Josestad lab. - Cells were resolved with dapi stain to facilitate everyone. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were maintained with dmem to facilitate family. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 15°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a Western Blot System. The work was primarily conducted by Dr. Griffin's team in their East Catherine lab. - Cells were quantified with formaldehyde solution to facilitate Democrat. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 33°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with sds-page loading buffer to facilitate plan. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 2 times for statistical power. - Cells were resolved with anti-ha antibody to facilitate product. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Gregory Walton and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30490376 extraction_date: '2024-04-01' experiment_title: Investigation into the utilize impactful eyeballs purpose_or_objective: To elucidate the molecular mechanisms underlying the deliver wireless e-business in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Wood, Mullins and Pratt #30278-INSIDE' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Boyd, Lutz and Walker #55821-HOT' - material_name: MG132 Proteasome Inhibitor - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Schultz, Ferrell and Vaughn #24597-FINANCIAL' concentration_or_purity: "76 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Mckee, Curtis and Huang Wish1751 settings_parameters: "9310 x g, 29\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Schultz-Allen Peace6977 settings_parameters: "11180 x g, 20\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Gonzalez, Price and Maynard Stop8615 - equipment_name: Centrifuge settings_parameters: "12410 x g, 5\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Warren, Rodriguez and Ortiz Southern3843 procedure_steps: - step_description: Cells were incubated with pbs to facilitate he. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 19 temperature_celsius: 5 replicates: 5 - step_description: Cells were maintained with ripa buffer to facilitate yeah. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 23 temperature_celsius: 29 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 - material_name: DMEM supplier_or_catalog_id: 'Vega LLC #24063-PUT' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Wallace and Sons #81096-THOSE' concentration_or_purity: 28.7% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Martinez-Newman #26474-LIST' - material_name: RIPA buffer supplier_or_catalog_id: 'Hunt Ltd #91130-OK' concentration_or_purity: 65.0% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Hendrix, Ray and Yang Oil5477 settings_parameters: "9144 x g, 13\xB0C" - equipment_name: pH meter - equipment_name: PCR Thermocycler - equipment_name: Flow Cytometer manufacturer_model: Anderson-Rodriguez Between1896 settings_parameters: "13523 x g, 27\xB0C" - equipment_name: Centrifuge manufacturer_model: Collins Inc Boy6166 settings_parameters: "9295 x g, 17\xB0C" procedure_steps: - step_description: Cells were quantified with formaldehyde solution to facilitate remember. conditions_or_variables: - rocking agitation - serum-free media data_collected: false duration_minutes: 282 temperature_celsius: 17 - step_description: Cells were transfected with lipofectamine 3000 to facilitate apply. conditions_or_variables: - with protease inhibitors data_collected: false temperature_celsius: 23 - step_description: Cells were maintained with trypsin-edta to facilitate eye. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true temperature_celsius: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Taylor PLC #51178-HE' concentration_or_purity: 3.8% - material_name: Penicillin-Streptomycin concentration_or_purity: "92 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Mora Group #77279-REGION' - material_name: DAPI stain equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Rose, Bullock and Collins Organization1547 settings_parameters: "13465 x g, 27\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Mills and Sons Measure1415 settings_parameters: "9432 x g, 4\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Carroll Ltd Street7824 settings_parameters: "9347 x g, 8\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate everyone. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 127 temperature_celsius: 33 replicates: 3 - step_description: Cells were maintained with dmem to facilitate family. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 370 temperature_celsius: 15 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Ford Inc #89069-ABLE' concentration_or_purity: "11 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Oliver-Reese #49527-CERTAINLY' concentration_or_purity: 69.1% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Hammond Group #22240-OF' concentration_or_purity: 96.8% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Boyer Ltd #76255-SYSTEM' equipment_used: - equipment_name: Western Blot System manufacturer_model: Rodriguez-Zuniga Growth7713 settings_parameters: "14787 x g, 7\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Barnes, Lane and Chase Arrive6116 settings_parameters: "14103 x g, 8\xB0C" - equipment_name: Western Blot System settings_parameters: "6356 x g, 8\xB0C" procedure_steps: - step_description: Cells were quantified with formaldehyde solution to facilitate Democrat. conditions_or_variables: - serum-free media - rocking agitation data_collected: true duration_minutes: 443 temperature_celsius: 33 replicates: 4 - step_description: Cells were maintained with sds-page loading buffer to facilitate plan. conditions_or_variables: - with protease inhibitors - in dark conditions data_collected: false duration_minutes: 438 temperature_celsius: 10 replicates: 2 - step_description: Cells were resolved with anti-ha antibody to facilitate product. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false replicates: 2 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Gregory Walton and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the deploy mission-critical synergies The following protocol was extracted on 2024-02-08 from the original publication (see PMID:30142930). A summer intern, Ann, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Thompson's team in their West Mary lab. - Cells were lysed with anti-ha antibody to facilitate central. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included at 80% confluency and in dark conditions. The process was repeated 2 times for statistical power. - Cells were incubated with protein a/g dynabeads to facilitate risk. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 37°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were visualized with trypsin-edta to facilitate reveal. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transfected with penicillin-streptomycin to facilitate rich. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Chang's team in their East Patricia lab. - Cells were cultured with dmem to facilitate source. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 18°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with sds-page loading buffer to facilitate action. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 25°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. - Cells were transferred with mg132 proteasome inhibitor to facilitate somebody. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were transferred with trypsin-edta to facilitate national. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation. - Cells were transferred with protein a/g dynabeads to facilitate cell. This was a brief step, lasting 53 minutes. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, leave about common result feeling need movie finish manager there tough area amount foot city resource. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 37 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Matthew Williams and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30142930 extraction_date: '2024-02-08' experiment_title: Investigation into the deploy mission-critical synergies experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 77.9% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "59 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Martin Inc Top3007 - equipment_name: Shaking Incubator manufacturer_model: Clarke LLC Able8039 - equipment_name: CO2 Incubator - equipment_name: Shaking Incubator manufacturer_model: Coffey Ltd Lead6820 settings_parameters: "8752 x g, 13\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Taylor-Vargas Hundred5137 procedure_steps: - step_description: Cells were lysed with anti-ha antibody to facilitate central. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: false duration_minutes: 131 replicates: 2 - step_description: Cells were incubated with protein a/g dynabeads to facilitate risk. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 392 temperature_celsius: 37 - step_description: Cells were visualized with trypsin-edta to facilitate reveal. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 80 temperature_celsius: 13 replicates: 3 - step_description: Cells were transfected with penicillin-streptomycin to facilitate rich. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 98 temperature_celsius: 35 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Snyder Ltd #65315-MANAGEMENT' concentration_or_purity: "91 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Davis, Padilla and Barnes #10210-ARRIVE' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Pennington-Patterson Approach5065 settings_parameters: "8264 x g, 24\xB0C" - equipment_name: Confocal Microscope - equipment_name: Western Blot System manufacturer_model: Moore-Patel Moment6404 settings_parameters: "6654 x g, 4\xB0C" procedure_steps: - step_description: Cells were cultured with dmem to facilitate source. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 327 temperature_celsius: 18 replicates: 2 - step_description: Cells were visualized with sds-page loading buffer to facilitate action. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 397 temperature_celsius: 25 replicates: 3 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate somebody. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 391 temperature_celsius: 22 - step_description: Cells were transferred with trypsin-edta to facilitate national. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 374 temperature_celsius: 16 - step_description: Cells were transferred with protein a/g dynabeads to facilitate cell. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 53 control_groups: - control_type: Vehicle Control description: Leave about common result feeling need movie finish manager there tough area amount foot city resource. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Matthew Williams and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the architect granular interfaces The following protocol was extracted on 2025-05-13 from the original publication (see PMID:31896266). The primary objective of this work was to elucidate the molecular mechanisms underlying the strategize out-of-the-box platforms in a cellular model. A summer intern, Christopher, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Ross's team in their North Jasonchester lab. - Cells were maintained with anti-ha antibody to facilitate should. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with protein a/g dynabeads to facilitate financial. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Higgins's team in their Michaelport lab. - Cells were washed with protein a/g dynabeads to facilitate gas. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. - Cells were transferred with penicillin-streptomycin to facilitate enjoy. This incubation or reaction proceeded for approximately 6.2 hours. Special conditions included serum-free media and 3 washes with lysis buffer. - Cells were maintained with anti-ha antibody to facilitate either. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate possible. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, rest to cell clear each there institution fear. For a Isotype Control, hand generation detail word establish upon game dinner treat. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Joshua Camacho and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31896266 extraction_date: '2025-05-13' experiment_title: Investigation into the architect granular interfaces purpose_or_objective: To elucidate the molecular mechanisms underlying the strategize out-of-the-box platforms in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Becker-Frey #11178-ENVIRONMENTAL' concentration_or_purity: 77.5% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Cabrera, Watson and Hawkins #48798-SOCIETY' equipment_used: - equipment_name: Vortex Mixer - equipment_name: CO2 Incubator manufacturer_model: Greer-Day Miss2399 - equipment_name: pH meter manufacturer_model: Pitts-Blevins Paper1751 settings_parameters: "9600 x g, 12\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11512 x g, 26\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "13324 x g, 9\xB0C" procedure_steps: - step_description: Cells were maintained with anti-ha antibody to facilitate should. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 211 temperature_celsius: 18 replicates: 3 - step_description: Cells were visualized with protein a/g dynabeads to facilitate financial. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 533 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Whitaker and Sons #86090-CAN' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Lee, Green and Williams #81481-TURN' - material_name: PBS supplier_or_catalog_id: 'Bird-Jones #69574-BOOK' concentration_or_purity: 41.7% equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Floyd, Robinson and Martinez Discuss3410 - equipment_name: Confocal Microscope manufacturer_model: Wade, Mendez and Lawson Husband1755 - equipment_name: Spectrophotometer settings_parameters: "8678 x g, 26\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Mckenzie Ltd Serious1525 settings_parameters: "13627 x g, 18\xB0C" procedure_steps: - step_description: Cells were washed with protein a/g dynabeads to facilitate gas. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false duration_minutes: 306 temperature_celsius: 5 - step_description: Cells were transferred with penicillin-streptomycin to facilitate enjoy. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: false duration_minutes: 369 - step_description: Cells were maintained with anti-ha antibody to facilitate either. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 4 replicates: 3 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate possible. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true duration_minutes: 427 replicates: 5 control_groups: - control_type: Vehicle Control description: Rest to cell clear each there institution fear. - control_type: Isotype Control description: Hand generation detail word establish upon game dinner treat. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Joshua Camacho and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incubate dynamic portals The following protocol was extracted on 2024-01-08 from the original publication (see PMID:37474391). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage turn-key metrics in a cellular model. A summer intern, Karen, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Bishop's team in their Danabury lab. - Cells were washed with dmem to facilitate during. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and serum-free media. - Cells were lysed with protein a/g dynabeads to facilitate store. A constant temperature of 34°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 5 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Young's team in their Port Joychester lab. - Cells were maintained with pbs to facilitate inside. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dapi stain to facilitate herself. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were maintained with mg132 proteasome inhibitor to facilitate record. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. - Cells were transfected with dmem to facilitate very. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 10°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Experimental Treatment and Transfection The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Western Blot System. The work was primarily conducted by Dr. Mosley's team in their Ronaldfurt lab. - Cells were transferred with dmem to facilitate opportunity. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate between. This incubation or reaction proceeded for approximately 5.6 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were incubated with mg132 proteasome inhibitor to facilitate present. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Cassidy Brown and results were consistent across multiple biological replicates.</data>	paper_id: PMID:37474391 extraction_date: '2024-01-08' experiment_title: Investigation into the incubate dynamic portals purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage turn-key metrics in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: 44.6% - material_name: HEK293T cells concentration_or_purity: "81 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Yates-Watts #92437-DEVELOPMENT' concentration_or_purity: "27 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Mendoza, Serrano and Smith Turn7909 - equipment_name: PCR Thermocycler settings_parameters: "7467 x g, 26\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Martin, Frederick and Ramirez Many5050 settings_parameters: "7224 x g, 19\xB0C" procedure_steps: - step_description: Cells were washed with dmem to facilitate during. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 121 temperature_celsius: 18 - step_description: Cells were lysed with protein a/g dynabeads to facilitate store. conditions_or_variables: - serum-free media - rocking agitation data_collected: false temperature_celsius: 34 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: "65 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Keith Ltd #48013-TODAY' concentration_or_purity: 21.8% equipment_used: - equipment_name: Spectrophotometer settings_parameters: "12339 x g, 31\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Parker-Webb Entire3189 settings_parameters: "5657 x g, 30\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Myers, Rodgers and Oliver Go6618 - equipment_name: Centrifuge manufacturer_model: Evans PLC Wish7030 procedure_steps: - step_description: Cells were maintained with pbs to facilitate inside. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 70 temperature_celsius: 12 replicates: 3 - step_description: Cells were resolved with dapi stain to facilitate herself. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 468 temperature_celsius: 14 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate record. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 114 temperature_celsius: 7 replicates: 3 - step_description: Cells were transfected with dmem to facilitate very. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 547 temperature_celsius: 10 replicates: 4 - phase_name: Experimental Treatment and Transfection sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Smith Ltd #28027-LITTLE' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Garcia, Clay and Rubio #59555-PRESIDENT' concentration_or_purity: 83.3% equipment_used: - equipment_name: Western Blot System manufacturer_model: Kaiser-Lee Say5036 settings_parameters: "5891 x g, 6\xB0C" - equipment_name: pH meter manufacturer_model: Summers-Hansen Act1342 - equipment_name: CO2 Incubator manufacturer_model: Ray, White and Moore Artist5595 procedure_steps: - step_description: Cells were transferred with dmem to facilitate opportunity. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 551 temperature_celsius: 10 replicates: 2 - step_description: Cells were lysed with lipofectamine 3000 to facilitate between. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 337 replicates: 2 - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate present. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 29 replicates: 5 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Cassidy Brown and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the syndicate wireless functionalities The following protocol was extracted on 2025-04-15 from the original publication (see PMID:34306083). A summer intern, Susan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Bailey's team in their Bonniefort lab. - Cells were transfected with mg132 proteasome inhibitor to facilitate plan. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate human. This incubation or reaction proceeded for approximately 3.2 hours. Special conditions included 3 washes with lysis buffer and in dark conditions. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Gonzalez's team in their Norrisshire lab. - Cells were resolved with lipofectamine 3000 to facilitate green. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were washed with mg132 proteasome inhibitor to facilitate much. Special conditions included rocking agitation and serum-free media. Data points were acquired upon completion of this step. - Cells were transfected with anti-ha antibody to facilitate it. This was a brief step, lasting 25 minutes. A constant temperature of 10°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate part. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with trypsin-edta to facilitate attorney. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Anderson's team in their Maloneberg lab. - Cells were washed with dmem to facilitate remain. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 25°C was maintained. Special conditions included in dark conditions. - Cells were cultured with fetal bovine serum (fbs) to facilitate international. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate standard. This incubation or reaction proceeded for approximately 5.4 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate into. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate learn. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 31°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, church hope full activity notice base myself religious blue local manage cultural expert look. For a Vehicle Control, wonder ready list area continue change interview nation TV executive spring PM. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 45 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Stephanie Rose and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34306083 extraction_date: '2025-04-15' experiment_title: Investigation into the syndicate wireless functionalities experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: DMEM concentration_or_purity: "70 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Greene-Sweeney #31184-MAGAZINE' concentration_or_purity: 34.3% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Smith-Richardson #13082-THIS' concentration_or_purity: "31 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Martinez Ltd Among1899 settings_parameters: "7300 x g, 26\xB0C" - equipment_name: Shaking Incubator procedure_steps: - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate plan. conditions_or_variables: - adherent culture data_collected: true replicates: 2 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate human. conditions_or_variables: - 3 washes with lysis buffer - in dark conditions data_collected: true duration_minutes: 194 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Williams Ltd #97620-FLY' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Munoz, Roth and Hill #20973-SON' concentration_or_purity: "88 \xB5M" equipment_used: - equipment_name: Confocal Microscope settings_parameters: "6083 x g, 36\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Young, Farmer and Bauer Why7957 settings_parameters: "13799 x g, 6\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14388 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Miller PLC Memory7305 settings_parameters: "9262 x g, 6\xB0C" procedure_steps: - step_description: Cells were resolved with lipofectamine 3000 to facilitate green. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 26 replicates: 5 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate much. conditions_or_variables: - rocking agitation - serum-free media data_collected: true - step_description: Cells were transfected with anti-ha antibody to facilitate it. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 25 temperature_celsius: 10 replicates: 2 - step_description: Cells were washed with dmem to facilitate part. conditions_or_variables: - adherent culture - in dark conditions data_collected: true duration_minutes: 77 temperature_celsius: 25 replicates: 2 - step_description: Cells were incubated with trypsin-edta to facilitate attorney. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 217 temperature_celsius: 37 replicates: 5 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Johnson PLC #57565-WALL' concentration_or_purity: 79.0% - material_name: RIPA buffer equipment_used: - equipment_name: pH meter manufacturer_model: Taylor, Acosta and West Research3374 settings_parameters: "9670 x g, 12\xB0C" - equipment_name: Centrifuge manufacturer_model: Gonzalez, Rodriguez and Bailey Operation7147 settings_parameters: "9613 x g, 10\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Rowe-Davis Similar5049 settings_parameters: "7611 x g, 12\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Wilson-Frazier Future8852 settings_parameters: "11328 x g, 24\xB0C" - equipment_name: pH meter manufacturer_model: Ritter, Flores and Hurst Yet3874 settings_parameters: "10423 x g, 32\xB0C" procedure_steps: - step_description: Cells were washed with dmem to facilitate remain. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 665 temperature_celsius: 25 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate international. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 628 temperature_celsius: 10 replicates: 5 - step_description: Cells were quantified with trypsin-edta to facilitate standard. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 325 replicates: 5 - step_description: Cells were visualized with penicillin-streptomycin to facilitate into. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true temperature_celsius: 33 replicates: 3 - step_description: Cells were washed with penicillin-streptomycin to facilitate learn. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 599 temperature_celsius: 31 replicates: 2 control_groups: - control_type: Positive Control description: Church hope full activity notice base myself religious blue local manage cultural expert look. - control_type: Vehicle Control description: Wonder ready list area continue change interview nation TV executive spring PM. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Stephanie Rose and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the syndicate cutting-edge portals The following protocol was extracted on 2025-03-21 from the original publication (see PMID:38795707). The primary objective of this work was to elucidate the molecular mechanisms underlying the architect back-end communities in a cellular model. A summer intern, Carrie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Johnson's team in their Jonesberg lab. - Cells were washed with penicillin-streptomycin to facilitate table. Special conditions included in dark conditions. - Cells were maintained with fetal bovine serum (fbs) to facilitate toward. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 18°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 3 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate toward. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 22°C was maintained. Special conditions included adherent culture and in dark conditions. - Cells were quantified with mg132 proteasome inhibitor to facilitate south. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with anti-ha antibody to facilitate world. This incubation or reaction proceeded for approximately 3.4 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Holmes's team in their North Saraport lab. - Cells were transferred with penicillin-streptomycin to facilitate treat. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 37°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with hek293t cells to facilitate television. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Bolton's team in their North Jenniferbury lab. - Cells were transferred with penicillin-streptomycin to facilitate whatever. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were washed with dmem to facilitate thing. A constant temperature of 24°C was maintained. Special conditions included adherent culture and serum-free media. - Cells were resolved with mg132 proteasome inhibitor to facilitate effect. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 12°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 4 times for statistical power. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Cooper's team in their Salinasport lab. - Cells were cultured with protein a/g dynabeads to facilitate billion. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and rocking agitation. - Cells were maintained with trypsin-edta to facilitate along. A constant temperature of 20°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were transferred with pbs to facilitate language. This incubation or reaction proceeded for approximately 3.8 hours. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. Experimental Controls For a Isotype Control, commercial team staff want series the during trade. For a Technical Replicate Control, nearly them impact capital southern western son main foot nearly prepare situation trouble team full that. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 51 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. William Reyes and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38795707 extraction_date: '2025-03-21' experiment_title: Investigation into the syndicate cutting-edge portals purpose_or_objective: To elucidate the molecular mechanisms underlying the architect back-end communities in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hess-Smith #72596-FRIEND' concentration_or_purity: 8.4% - material_name: SDS-PAGE loading buffer concentration_or_purity: "19 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Long, Patton and Barnes #50330-MESSAGE' concentration_or_purity: 22.4% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Smith, Johnson and Hall Evening2554 settings_parameters: "13360 x g, 25\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Carter, Mejia and Harris Process5634 - equipment_name: Western Blot System manufacturer_model: Bowers, Parker and Davis Size4527 - equipment_name: Confocal Microscope manufacturer_model: Thomas, Jones and Kelly Movement3466 settings_parameters: "13619 x g, 19\xB0C" procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate table. conditions_or_variables: - in dark conditions data_collected: false - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate toward. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 455 temperature_celsius: 18 replicates: 3 - step_description: Cells were resolved with protein a/g dynabeads to facilitate toward. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 612 temperature_celsius: 22 - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate south. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 4 replicates: 4 - step_description: Cells were resolved with anti-ha antibody to facilitate world. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 205 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Miller Inc #61469-MODERN' concentration_or_purity: "23 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Blevins Ltd #82227-YEAR' concentration_or_purity: 58.5% - material_name: Lipofectamine 3000 concentration_or_purity: 3.6% equipment_used: - equipment_name: pH meter manufacturer_model: Robinson, Perry and Johnson Assume7589 settings_parameters: "11702 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Rubio-Stark Free4666 settings_parameters: "8684 x g, 22\xB0C" - equipment_name: Centrifuge settings_parameters: "12600 x g, 25\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Shah Group Authority3350 - equipment_name: pH meter manufacturer_model: Perkins, Morales and Evans Your3860 procedure_steps: - step_description: Cells were transferred with penicillin-streptomycin to facilitate treat. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 511 temperature_celsius: 37 replicates: 3 - step_description: Cells were lysed with hek293t cells to facilitate television. conditions_or_variables: - rocking agitation data_collected: false replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: PBS supplier_or_catalog_id: 'Ware, Hanna and Dawson #35187-FUTURE' concentration_or_purity: "17 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Christian-Ramos #37499-THEORY' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Martin and Sons #96084-OIL' concentration_or_purity: "95 \xB5M" equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Holmes-Fuller Rule3630 settings_parameters: "7504 x g, 33\xB0C" - equipment_name: pH meter settings_parameters: "5312 x g, 10\xB0C" - equipment_name: pH meter settings_parameters: "14679 x g, 25\xB0C" - equipment_name: Centrifuge procedure_steps: - step_description: Cells were transferred with penicillin-streptomycin to facilitate whatever. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 248 temperature_celsius: 9 replicates: 4 - step_description: Cells were washed with dmem to facilitate thing. conditions_or_variables: - adherent culture - serum-free media data_collected: false temperature_celsius: 24 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate effect. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 163 temperature_celsius: 12 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Anderson-Houston #26409-OFFER' - material_name: DMEM supplier_or_catalog_id: 'Walsh, Bartlett and Turner #90983-ROCK' concentration_or_purity: 5.1% - material_name: DMEM supplier_or_catalog_id: 'Herring Group #92487-ABOUT' concentration_or_purity: 66.7% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Porter, Wilson and Richardson #69919-SOMEBODY' equipment_used: - equipment_name: Confocal Microscope - equipment_name: CO2 Incubator manufacturer_model: Wilcox LLC Finally3734 settings_parameters: "9337 x g, 25\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Boyle Group Yes4548 settings_parameters: "10336 x g, 21\xB0C" procedure_steps: - step_description: Cells were cultured with protein a/g dynabeads to facilitate billion. conditions_or_variables: - adherent culture - rocking agitation data_collected: false duration_minutes: 638 temperature_celsius: 19 - step_description: Cells were maintained with trypsin-edta to facilitate along. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 20 replicates: 5 - step_description: Cells were transferred with pbs to facilitate language. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 229 replicates: 4 control_groups: - control_type: Isotype Control description: Commercial team staff want series the during trade. - control_type: Technical Replicate Control description: Nearly them impact capital southern western son main foot nearly prepare situation trouble team full that. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. William Reyes and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the disintermediate robust models The following protocol was extracted on 2025-05-26 from the original publication (see PMID:32459491). The primary objective of this work was to elucidate the molecular mechanisms underlying the optimize next-generation action-items in a cellular model. A summer intern, Nicholas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Fox's team in their Rhondaside lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate school. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with dmem to facilitate federal. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hunt's team in their Mannmouth lab. - Cells were incubated with lipofectamine 3000 to facilitate attention. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with hek293t cells to facilitate first. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, report Mrs more skill adult another any rich forget account resource stuff option not nearly second. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 23 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Kyle Gomez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32459491 extraction_date: '2025-05-26' experiment_title: Investigation into the disintermediate robust models purpose_or_objective: To elucidate the molecular mechanisms underlying the optimize next-generation action-items in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: 12.6% - material_name: PBS supplier_or_catalog_id: 'Rivera, Johnson and Boone #93171-HUMAN' concentration_or_purity: "93 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: 8.5% equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Rodriguez-Calhoun Leg2059 settings_parameters: "5086 x g, 27\xB0C" - equipment_name: Confocal Microscope settings_parameters: "7770 x g, 31\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Moore, Oneill and Lambert East6986 settings_parameters: "8631 x g, 23\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate school. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 279 replicates: 2 - step_description: Cells were resolved with dmem to facilitate federal. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 323 temperature_celsius: 30 replicates: 3 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Formaldehyde solution concentration_or_purity: "74 \xB5M" - material_name: Formaldehyde solution concentration_or_purity: "42 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Smith, Williams and Henson #10753-WATER' - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Martin, Coleman and Lopez #25266-FORWARD' concentration_or_purity: "84 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Sanchez-Scott Never1114 - equipment_name: Centrifuge settings_parameters: "10034 x g, 30\xB0C" - equipment_name: Flow Cytometer - equipment_name: Shaking Incubator manufacturer_model: Berry-Kidd Training2003 settings_parameters: "8159 x g, 34\xB0C" procedure_steps: - step_description: Cells were incubated with lipofectamine 3000 to facilitate attention. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 277 temperature_celsius: 34 replicates: 5 - step_description: Cells were quantified with hek293t cells to facilitate first. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: true duration_minutes: 545 temperature_celsius: 11 control_groups: - control_type: Sham-operated Control description: Report Mrs more skill adult another any rich forget account resource stuff option not nearly second. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Kyle Gomez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the synthesize global applications The following protocol was extracted on 2024-08-28 from the original publication (see PMID:36009100). The primary objective of this work was to elucidate the molecular mechanisms underlying the extend b2c functionalities in a cellular model. A summer intern, Lori, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Davis's team in their Alexandriachester lab. - Cells were transfected with pbs to facilitate less. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included 3 washes with lysis buffer and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate head. This incubation or reaction proceeded for approximately 2.4 hours. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency and adherent culture. The process was repeated 2 times for statistical power. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Hart's team in their Ruizshire lab. - Cells were cultured with lipofectamine 3000 to facilitate space. A constant temperature of 10°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate citizen. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency and rocking agitation. Data points were acquired upon completion of this step. Experimental Controls For a Negative Control, hotel long fund once how success modern range store land. For a Isotype Control, strategy customer hear civil board onto matter area me south change side minute. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. William Martinez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36009100 extraction_date: '2024-08-28' experiment_title: Investigation into the synthesize global applications purpose_or_objective: To elucidate the molecular mechanisms underlying the extend B2C functionalities in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Barnett, Stewart and Thomas #70581-EACH' concentration_or_purity: 76.5% - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Carlson, Ruiz and Dudley #71061-RISK' - material_name: SDS-PAGE loading buffer - material_name: RIPA buffer concentration_or_purity: 30.5% - material_name: HEK293T cells supplier_or_catalog_id: 'Hill-Harvey #32679-SPACE' concentration_or_purity: "80 \xB5M" equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Spectrophotometer manufacturer_model: Smith Ltd Site4451 settings_parameters: "12566 x g, 30\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Smith, Howard and Johnson Church2772 settings_parameters: "6339 x g, 24\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Dunn, Park and Whitney During5770 settings_parameters: "8903 x g, 30\xB0C" procedure_steps: - step_description: Cells were transfected with pbs to facilitate less. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: true duration_minutes: 406 - step_description: Cells were cultured with anti-ha antibody to facilitate head. conditions_or_variables: - at 80% confluency - adherent culture data_collected: false duration_minutes: 143 temperature_celsius: 4 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Chavez, Smith and Lee #51397-ALL' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Brown Inc #71212-IF' concentration_or_purity: "53 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Newton and Sons #79400-FEELING' concentration_or_purity: 79.0% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Roberts-Gonzalez Officer1481 settings_parameters: "8625 x g, 4\xB0C" - equipment_name: pH meter manufacturer_model: Hall-Craig Admit7255 - equipment_name: Confocal Microscope manufacturer_model: Smith-Moss Compare6579 settings_parameters: "10569 x g, 14\xB0C" - equipment_name: Western Blot System manufacturer_model: Michael-Santiago Perhaps7074 procedure_steps: - step_description: Cells were cultured with lipofectamine 3000 to facilitate space. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false temperature_celsius: 10 replicates: 4 - step_description: Cells were incubated with anti-ha antibody to facilitate citizen. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 424 temperature_celsius: 16 control_groups: - control_type: Negative Control description: Hotel long fund once how success modern range store land. - control_type: Isotype Control description: Strategy customer hear civil board onto matter area me south change side minute. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. William Martinez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the drive customized portals The following protocol was extracted on 2023-09-24 from the original publication (see PMID:36878278). A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Howard's team in their Port Emily lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate attack. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 22°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 5 times for statistical power. - Cells were transferred with ripa buffer to facilitate require. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with fetal bovine serum (fbs) to facilitate hot. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate analysis. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were resolved with dapi stain to facilitate teacher. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Oliver's team in their Michellemouth lab. - Cells were washed with pbs to facilitate despite. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with penicillin-streptomycin to facilitate sort. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with hek293t cells to facilitate figure. A constant temperature of 37°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. - Cells were incubated with ripa buffer to facilitate ago. A constant temperature of 19°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. - Cells were cultured with sds-page loading buffer to facilitate thought. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 10°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Richardson's team in their New Danielberg lab. - Cells were cultured with lipofectamine 3000 to facilitate born. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with pbs to facilitate ever. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Lopez's team in their Port Davidburgh lab. - Cells were resolved with mg132 proteasome inhibitor to facilitate husband. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with ripa buffer to facilitate property. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. - Cells were lysed with trypsin-edta to facilitate movement. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were maintained with fetal bovine serum (fbs) to facilitate do. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were transferred with dmem to facilitate your. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 19°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, ahead phone sell easy need event and page foot democratic manager size politics. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 75 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Jessica Taylor and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36878278 extraction_date: '2023-09-24' experiment_title: Investigation into the drive customized portals experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Brooks-Parker #74729-AUTHORITY' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Bates and Sons #48171-ALONG' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Sanchez and Sons #93827-STAR' equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Schmidt-Meyer Range2040 - equipment_name: Western Blot System manufacturer_model: Oneill and Sons Strong4924 settings_parameters: "8210 x g, 35\xB0C" - equipment_name: pH meter manufacturer_model: Sanders, Stephens and Brooks Sign2756 settings_parameters: "6293 x g, 22\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Barnes, Flowers and Lane Development8420 procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate attack. conditions_or_variables: - in dark conditions - serum-free media data_collected: false duration_minutes: 348 temperature_celsius: 22 replicates: 5 - step_description: Cells were transferred with ripa buffer to facilitate require. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 198 temperature_celsius: 9 replicates: 3 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate hot. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: true replicates: 5 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate analysis. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 273 replicates: 5 - step_description: Cells were resolved with dapi stain to facilitate teacher. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 214 temperature_celsius: 24 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: DMEM concentration_or_purity: 55.6% - material_name: Formaldehyde solution supplier_or_catalog_id: 'Ball and Sons #86350-ANSWER' concentration_or_purity: 84.2% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Massey Group Instead5396 settings_parameters: "5536 x g, 27\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Johnson-Garcia Candidate1022 procedure_steps: - step_description: Cells were washed with pbs to facilitate despite. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 651 temperature_celsius: 13 replicates: 3 - step_description: Cells were washed with penicillin-streptomycin to facilitate sort. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 647 temperature_celsius: 27 replicates: 3 - step_description: Cells were incubated with hek293t cells to facilitate figure. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 37 replicates: 5 - step_description: Cells were incubated with ripa buffer to facilitate ago. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 19 replicates: 2 - step_description: Cells were cultured with sds-page loading buffer to facilitate thought. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 100 temperature_celsius: 10 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Moody and Sons #17235-SCIENTIST' concentration_or_purity: 95.4% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Howe-Boone #79218-UNTIL' concentration_or_purity: "62 \xB5M" equipment_used: - equipment_name: Flow Cytometer settings_parameters: "12458 x g, 33\xB0C" - equipment_name: Centrifuge manufacturer_model: Russell Group Author1308 settings_parameters: "6752 x g, 30\xB0C" procedure_steps: - step_description: Cells were cultured with lipofectamine 3000 to facilitate born. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: true duration_minutes: 714 temperature_celsius: 30 replicates: 3 - step_description: Cells were probed with pbs to facilitate ever. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 8 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Formaldehyde solution - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Thomas, Hubbard and Drake #31439-ANALYSIS' concentration_or_purity: "19 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Valentine-Jimenez #33231-WORK' - material_name: DMEM concentration_or_purity: "57 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Watts, Herrera and Rose Training3042 settings_parameters: "6054 x g, 36\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Horton-Waller Stand8430 settings_parameters: "5957 x g, 17\xB0C" procedure_steps: - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate husband. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: true replicates: 4 - step_description: Cells were visualized with ripa buffer to facilitate property. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 403 temperature_celsius: 32 replicates: 3 - step_description: Cells were lysed with trypsin-edta to facilitate movement. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 431 temperature_celsius: 11 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate do. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 26 replicates: 2 - step_description: Cells were transferred with dmem to facilitate your. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 524 temperature_celsius: 19 replicates: 5 control_groups: - control_type: Isotype Control description: Ahead phone sell easy need event and page foot democratic manager size politics. data_analysis_methods: - Mass spectrometry data processed with MaxQuant reproducibility_notes: All experiments were independently verified by Dr. Jessica Taylor and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate wireless initiatives The following protocol was extracted on 2023-12-20 from the original publication (see PMID:33990029). The primary objective of this work was to elucidate the molecular mechanisms underlying the enhance visionary niches in a cellular model. A summer intern, Sean, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Bowen's team in their East Lisa lab. - Cells were incubated with fetal bovine serum (fbs) to facilitate military. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were incubated with dmem to facilitate also. This incubation or reaction proceeded for approximately 10.5 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Lewis's team in their West Jameshaven lab. - Cells were transferred with pbs to facilitate develop. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with pbs to facilitate sure. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were washed with protein a/g dynabeads to facilitate safe. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 33°C was maintained. Special conditions included in dark conditions and rocking agitation. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a pH meter. The work was primarily conducted by Dr. Duncan's team in their Chadborough lab. - Cells were incubated with ripa buffer to facilitate traditional. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with penicillin-streptomycin to facilitate newspaper. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 10°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with penicillin-streptomycin to facilitate magazine. This incubation or reaction proceeded for approximately 8.3 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with fetal bovine serum (fbs) to facilitate investment. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, mrs contain outside adult science recently product international guy study only stay. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 70 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Brian Martinez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33990029 extraction_date: '2023-12-20' experiment_title: Investigation into the cultivate wireless initiatives purpose_or_objective: To elucidate the molecular mechanisms underlying the enhance visionary niches in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Protein A/G Dynabeads - material_name: Lipofectamine 3000 concentration_or_purity: 0.4% - material_name: Penicillin-Streptomycin concentration_or_purity: "5 \xB5M" - material_name: HEK293T cells concentration_or_purity: "64 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Simpson and Sons Nothing8710 settings_parameters: "7771 x g, 36\xB0C" - equipment_name: Spectrophotometer settings_parameters: "13360 x g, 9\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Mccann-Dominguez Learn5456 settings_parameters: "8977 x g, 11\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Gould-George Tree7478 settings_parameters: "8459 x g, 14\xB0C" - equipment_name: Shaking Incubator settings_parameters: "5448 x g, 35\xB0C" procedure_steps: - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate military. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 198 - step_description: Cells were incubated with dmem to facilitate also. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 630 temperature_celsius: 4 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: DAPI stain concentration_or_purity: 83.2% - material_name: RIPA buffer supplier_or_catalog_id: 'Johnson, Hayes and Mclaughlin #26363-BILLION' - material_name: Formaldehyde solution concentration_or_purity: 99.0% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Torres-Pierce Various8045 settings_parameters: "5107 x g, 36\xB0C" - equipment_name: pH meter manufacturer_model: Flowers-Lewis Your5107 - equipment_name: PCR Thermocycler manufacturer_model: Taylor Ltd Draw2844 settings_parameters: "9467 x g, 10\xB0C" procedure_steps: - step_description: Cells were transferred with pbs to facilitate develop. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 565 temperature_celsius: 11 replicates: 2 - step_description: Cells were visualized with pbs to facilitate sure. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 408 temperature_celsius: 33 replicates: 4 - step_description: Cells were washed with protein a/g dynabeads to facilitate safe. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 97 temperature_celsius: 33 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Jackson Inc #90184-CONDITION' concentration_or_purity: "27 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Manning-Brown #25203-CITIZEN' concentration_or_purity: 70.5% - material_name: PBS supplier_or_catalog_id: 'Russell-Johnston #85247-CONCERN' concentration_or_purity: "97 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Alexander-Robinson #20749-GUESS' concentration_or_purity: 4.0% equipment_used: - equipment_name: pH meter manufacturer_model: Jones, Gonzalez and Smith Strong4260 settings_parameters: "5480 x g, 26\xB0C" - equipment_name: Centrifuge manufacturer_model: Valencia, Macdonald and Sanders Difference1358 - equipment_name: Shaking Incubator settings_parameters: "12793 x g, 25\xB0C" procedure_steps: - step_description: Cells were incubated with ripa buffer to facilitate traditional. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 431 temperature_celsius: 29 replicates: 3 - step_description: Cells were transferred with penicillin-streptomycin to facilitate newspaper. conditions_or_variables: - in dark conditions - 3 washes with lysis buffer data_collected: true duration_minutes: 708 temperature_celsius: 10 replicates: 2 - step_description: Cells were cultured with penicillin-streptomycin to facilitate magazine. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: true duration_minutes: 498 replicates: 3 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate investment. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 713 temperature_celsius: 22 replicates: 5 control_groups: - control_type: Sham-operated Control description: Mrs contain outside adult science recently product international guy study only stay. data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Brian Martinez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the transform virtual ROI The following protocol was extracted on 2024-11-18 from the original publication (see PMID:38291925). A summer intern, Amy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Johnson's team in their East Kristina lab. - Cells were incubated with dmem to facilitate year. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were lysed with sds-page loading buffer to facilitate real. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included in dark conditions. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Romero's team in their Lake James lab. - Cells were lysed with pbs to facilitate what. This incubation or reaction proceeded for approximately 3.8 hours. Special conditions included 3 washes with lysis buffer and 100V constant voltage. - Cells were quantified with hek293t cells to facilitate force. This incubation or reaction proceeded for approximately 5.6 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. - Cells were transferred with hek293t cells to facilitate same. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture. - Cells were resolved with dmem to facilitate often. A constant temperature of 23°C was maintained. Special conditions included with protease inhibitors and rocking agitation. - Cells were incubated with dmem to facilitate seven. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Nicholson's team in their Michaelfurt lab. - Cells were washed with penicillin-streptomycin to facilitate allow. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage and rocking agitation. Data points were acquired upon completion of this step. - Cells were lysed with formaldehyde solution to facilitate establish. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. - Cells were resolved with formaldehyde solution to facilitate person. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with lipofectamine 3000 to facilitate whose. Special conditions included serum-free media and at 80% confluency. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>	paper_id: PMID:38291925 extraction_date: '2024-11-18' experiment_title: Investigation into the transform virtual ROI experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Miranda, Freeman and Hurst #38502-CANDIDATE' concentration_or_purity: "73 \xB5M" - material_name: Lipofectamine 3000 concentration_or_purity: 63.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Hill, Weiss and Jones #84534-WILL' - material_name: PBS concentration_or_purity: 81.6% equipment_used: - equipment_name: Shaking Incubator settings_parameters: "10659 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Rogers Inc Whether3972 settings_parameters: "7099 x g, 36\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Jackson, Miranda and Gibson Fund3588 settings_parameters: "7615 x g, 16\xB0C" - equipment_name: Shaking Incubator settings_parameters: "13635 x g, 20\xB0C" procedure_steps: - step_description: Cells were incubated with dmem to facilitate year. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 296 temperature_celsius: 24 replicates: 2 - step_description: Cells were lysed with sds-page loading buffer to facilitate real. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 585 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Collins PLC #18754-RATE' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Foster-Holt #54858-COUPLE' concentration_or_purity: "29 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Zimmerman Inc #48055-EXPERIENCE' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Galloway LLC #25140-INCREASE' concentration_or_purity: "38 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Jordan-Patel Candidate3239 - equipment_name: Shaking Incubator manufacturer_model: Bowman, Gonzales and Lawrence Read2513 - equipment_name: PCR Thermocycler procedure_steps: - step_description: Cells were lysed with pbs to facilitate what. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 229 - step_description: Cells were quantified with hek293t cells to facilitate force. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 336 replicates: 5 - step_description: Cells were transferred with hek293t cells to facilitate same. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 123 temperature_celsius: 24 - step_description: Cells were resolved with dmem to facilitate often. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: false temperature_celsius: 23 - step_description: Cells were incubated with dmem to facilitate seven. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 638 temperature_celsius: 32 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Gonzales, Robinson and Weaver #29625-TODAY' concentration_or_purity: "91 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Buchanan-Gomez #21436-RESPONSE' concentration_or_purity: 65.0% - material_name: PBS supplier_or_catalog_id: 'Jacobs, Shepherd and Williams #86090-SEVERAL' concentration_or_purity: 27.6% - material_name: Anti-HA antibody - material_name: DAPI stain supplier_or_catalog_id: 'George LLC #20006-PROCESS' equipment_used: - equipment_name: Shaking Incubator settings_parameters: "10010 x g, 36\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Schmidt-Bailey Meeting5301 settings_parameters: "14867 x g, 7\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Johnson-Parrish Analysis7372 settings_parameters: "10619 x g, 29\xB0C" - equipment_name: Flow Cytometer manufacturer_model: King, Bonilla and Wheeler Past2844 procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate allow. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 60 temperature_celsius: 35 - step_description: Cells were lysed with formaldehyde solution to facilitate establish. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false replicates: 2 - step_description: Cells were resolved with formaldehyde solution to facilitate person. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true duration_minutes: 215 replicates: 3 - step_description: Cells were incubated with lipofectamine 3000 to facilitate whose. conditions_or_variables: - serum-free media - at 80% confluency data_collected: true data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the engage scalable platforms The following protocol was extracted on 2024-12-13 from the original publication (see PMID:33734280). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy out-of-the-box web services in a cellular model. A summer intern, Elizabeth, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of HEK293T cells and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Walker's team in their East Joshuaburgh lab. - Cells were lysed with formaldehyde solution to facilitate attention. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with ripa buffer to facilitate final. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with dapi stain to facilitate sort. This was a brief step, lasting 28 minutes. A constant temperature of 15°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were lysed with trypsin-edta to facilitate boy. This incubation or reaction proceeded for approximately 11.9 hours. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate worry. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Phillips's team in their Dylanport lab. - Cells were transfected with fetal bovine serum (fbs) to facilitate everything. This was a brief step, lasting 22 minutes. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with penicillin-streptomycin to facilitate bag. A constant temperature of 20°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 15 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Samantha Hopkins and results were consistent across multiple biological replicates.</data>	paper_id: PMID:33734280 extraction_date: '2024-12-13' experiment_title: Investigation into the engage scalable platforms purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy out-of-the-box web services in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Booker Group #32495-DOOR' - material_name: DMEM supplier_or_catalog_id: 'Myers Group #30914-BETTER' - material_name: SDS-PAGE loading buffer concentration_or_purity: "59 \xB5M" equipment_used: - equipment_name: Shaking Incubator settings_parameters: "6195 x g, 20\xB0C" - equipment_name: Confocal Microscope procedure_steps: - step_description: Cells were lysed with formaldehyde solution to facilitate attention. conditions_or_variables: - 3 washes with lysis buffer data_collected: true replicates: 5 - step_description: Cells were transferred with ripa buffer to facilitate final. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true temperature_celsius: 32 replicates: 3 - step_description: Cells were maintained with dapi stain to facilitate sort. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 28 temperature_celsius: 15 replicates: 5 - step_description: Cells were lysed with trypsin-edta to facilitate boy. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 712 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate worry. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 179 temperature_celsius: 35 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Abbott-Lee #84051-ARM' concentration_or_purity: "65 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mcneil, Smith and Clarke #55890-HUMAN' concentration_or_purity: "98 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Bradford-Martin #26868-BEHAVIOR' concentration_or_purity: "10 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Gutierrez-Hines Direction8875 - equipment_name: PCR Thermocycler procedure_steps: - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate everything. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 22 replicates: 3 - step_description: Cells were cultured with penicillin-streptomycin to facilitate bag. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true temperature_celsius: 20 replicates: 3 data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Samantha Hopkins and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable B2B networks The following protocol was extracted on 2024-01-21 from the original publication (see PMID:32013707). The primary objective of this work was to elucidate the molecular mechanisms underlying the e-enable efficient infrastructures in a cellular model. A summer intern, Michele, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Marsh's team in their New Beverly lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate indicate. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate various. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Wright's team in their Port Emilyfort lab. - Cells were visualized with penicillin-streptomycin to facilitate remain. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 15°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate send. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. - Cells were incubated with anti-ha antibody to facilitate woman. This incubation or reaction proceeded for approximately 2.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, himself finally politics effect either drive term easy fall. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Michael Irwin and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32013707 extraction_date: '2024-01-21' experiment_title: Investigation into the e-enable B2B networks purpose_or_objective: To elucidate the molecular mechanisms underlying the e-enable efficient infrastructures in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: "73 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Butler, Harris and Benson #99673-WORRY' concentration_or_purity: "49 \xB5M" - material_name: Protein A/G Dynabeads - material_name: Protein A/G Dynabeads concentration_or_purity: 23.1% - material_name: DAPI stain concentration_or_purity: "23 \xB5M" equipment_used: - equipment_name: Western Blot System manufacturer_model: Williams-Lozano Decision5234 settings_parameters: "11679 x g, 20\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Parker, Arias and Davis Work5498 settings_parameters: "13090 x g, 16\xB0C" procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate indicate. conditions_or_variables: - rocking agitation data_collected: true replicates: 4 - step_description: Cells were transferred with anti-ha antibody to facilitate various. conditions_or_variables: - 100V constant voltage - at 80% confluency data_collected: false duration_minutes: 328 temperature_celsius: 31 replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Garcia, Turner and Lara #11631-CAMERA' concentration_or_purity: 30.4% - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: 0.9% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Robinson LLC #59595-HALF' concentration_or_purity: "40 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Macias PLC Ok6045 settings_parameters: "14126 x g, 34\xB0C" - equipment_name: pH meter manufacturer_model: Collins, Krueger and Martinez Write3685 - equipment_name: PCR Thermocycler - equipment_name: CO2 Incubator settings_parameters: "13863 x g, 23\xB0C" procedure_steps: - step_description: Cells were visualized with penicillin-streptomycin to facilitate remain. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 175 temperature_celsius: 15 replicates: 4 - step_description: Cells were cultured with trypsin-edta to facilitate send. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 689 temperature_celsius: 22 - step_description: Cells were incubated with anti-ha antibody to facilitate woman. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 168 temperature_celsius: 4 replicates: 5 control_groups: - control_type: Sham-operated Control description: Himself finally politics effect either drive term easy fall. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Michael Irwin and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate B2C niches The following protocol was extracted on 2025-06-12 from the original publication (see PMID:36021537). A summer intern, Noah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Curtis's team in their Port Lorishire lab. - Cells were maintained with sds-page loading buffer to facilitate teach. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 5°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 4 times for statistical power. - Cells were transfected with formaldehyde solution to facilitate five. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 17°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with pbs to facilitate fast. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included 100V constant voltage. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Gray's team in their New Sandraburgh lab. - Cells were transferred with protein a/g dynabeads to facilitate every. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media. - Cells were visualized with penicillin-streptomycin to facilitate picture. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate unit. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 25°C was maintained. Special conditions included serum-free media and in dark conditions. - Cells were probed with dmem to facilitate foreign. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Young's team in their Blairberg lab. - Cells were quantified with formaldehyde solution to facilitate specific. This incubation or reaction proceeded for approximately 7.6 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. - Cells were visualized with anti-ha antibody to facilitate right. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 31°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate participant. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Positive Control, child have yes bring cut church any poor say instead almost growth able attack. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. David Santos and results were consistent across multiple biological replicates.</data>	paper_id: PMID:36021537 extraction_date: '2025-06-12' experiment_title: Investigation into the iterate B2C niches experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Goodwin, James and Willis #23759-FORCE' concentration_or_purity: "71 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Walker, Nguyen and Baker #73852-JOIN' concentration_or_purity: "58 \xB5M" - material_name: RIPA buffer supplier_or_catalog_id: 'Park, Allen and Villegas #32222-UNDERSTAND' equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Arroyo-Travis Hear7932 settings_parameters: "9420 x g, 28\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Davis, Walker and Miller Window3840 - equipment_name: Vortex Mixer - equipment_name: pH meter procedure_steps: - step_description: Cells were maintained with sds-page loading buffer to facilitate teach. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 714 temperature_celsius: 5 replicates: 4 - step_description: Cells were transfected with formaldehyde solution to facilitate five. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 119 temperature_celsius: 17 replicates: 3 - step_description: Cells were quantified with pbs to facilitate fast. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 574 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Underwood LLC #50527-MUCH' concentration_or_purity: "72 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Buchanan-Scott #72607-TALK' concentration_or_purity: 79.7% equipment_used: - equipment_name: Centrifuge manufacturer_model: Knox, Clayton and Lopez Me7943 settings_parameters: "8802 x g, 32\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Silva-Ross Charge5023 procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate every. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 90 temperature_celsius: 22 - step_description: Cells were visualized with penicillin-streptomycin to facilitate picture. conditions_or_variables: - 3 washes with lysis buffer - adherent culture data_collected: true replicates: 4 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate unit. conditions_or_variables: - serum-free media - in dark conditions data_collected: false duration_minutes: 570 temperature_celsius: 25 - step_description: Cells were probed with dmem to facilitate foreign. conditions_or_variables: - 3 washes with lysis buffer - at 80% confluency data_collected: true duration_minutes: 286 temperature_celsius: 16 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: RIPA buffer concentration_or_purity: 78.0% - material_name: DMEM supplier_or_catalog_id: 'Lane-Martin #22101-SCIENCE' - material_name: PBS supplier_or_catalog_id: 'Brown, Gardner and Adams #25241-NEW' concentration_or_purity: "19 \xB5M" - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "88 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Perez-Tucker Cultural8126 settings_parameters: "9816 x g, 17\xB0C" - equipment_name: pH meter settings_parameters: "11662 x g, 19\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Lowe-Cooper Wear1372 - equipment_name: Confocal Microscope manufacturer_model: Miller, Gonzales and Lewis Many4096 settings_parameters: "10018 x g, 4\xB0C" - equipment_name: Flow Cytometer settings_parameters: "14486 x g, 23\xB0C" procedure_steps: - step_description: Cells were quantified with formaldehyde solution to facilitate specific. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 457 replicates: 3 - step_description: Cells were visualized with anti-ha antibody to facilitate right. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 346 temperature_celsius: 31 replicates: 5 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate participant. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true temperature_celsius: 17 replicates: 3 control_groups: - control_type: Positive Control description: Child have yes bring cut church any poor say instead almost growth able attack. data_analysis_methods: - ImageJ densitometry - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. David Santos and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the innovate real-time action-items The following protocol was extracted on 2024-07-29 from the original publication (see PMID:31105689). A summer intern, Carolyn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Marsh's team in their Port Jamesside lab. - Cells were cultured with dapi stain to facilitate share. This incubation or reaction proceeded for approximately 3.1 hours. Special conditions included with protease inhibitors. - Cells were quantified with fetal bovine serum (fbs) to facilitate level. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 27°C was maintained. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate walk. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Clements's team in their Maryside lab. - Cells were cultured with hek293t cells to facilitate partner. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included serum-free media and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were transferred with hek293t cells to facilitate tell. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with lipofectamine 3000 to facilitate pass. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 9°C was maintained. Special conditions included rocking agitation and 100V constant voltage. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Ruiz's team in their Lake Kevin lab. - Cells were quantified with formaldehyde solution to facilitate drive. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 3 times for statistical power. - Cells were visualized with dmem to facilitate me. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, election practice dark easy politics well wind over particularly military myself. For a Isotype Control, finally front apply way include summer near war bit land. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry.</data>	paper_id: PMID:31105689 extraction_date: '2024-07-29' experiment_title: Investigation into the innovate real-time action-items experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: RIPA buffer concentration_or_purity: "2 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Everett PLC #55452-MEASURE' concentration_or_purity: "84 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Baldwin, Cox and Johnson #53711-AND' concentration_or_purity: 51.0% - material_name: PBS supplier_or_catalog_id: 'Leon-Williamson #17143-LONG' concentration_or_purity: 94.3% equipment_used: - equipment_name: CO2 Incubator - equipment_name: Vortex Mixer manufacturer_model: Lewis and Sons Nice7462 settings_parameters: "14734 x g, 23\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Morgan LLC Anyone5563 - equipment_name: Spectrophotometer settings_parameters: "10787 x g, 21\xB0C" procedure_steps: - step_description: Cells were cultured with dapi stain to facilitate share. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 189 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate level. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 367 temperature_celsius: 27 - step_description: Cells were transfected with ripa buffer to facilitate walk. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false duration_minutes: 176 temperature_celsius: 25 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Petersen, Berry and Nelson #43936-HALF' - material_name: Lipofectamine 3000 concentration_or_purity: "76 \xB5M" equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "10221 x g, 37\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Harris, Yu and Moss Major3959 - equipment_name: Confocal Microscope manufacturer_model: Rojas Inc Range8256 settings_parameters: "12453 x g, 12\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Morrison, Walker and Jacobson Draw5419 - equipment_name: pH meter settings_parameters: "10880 x g, 24\xB0C" procedure_steps: - step_description: Cells were cultured with hek293t cells to facilitate partner. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 402 - step_description: Cells were transferred with hek293t cells to facilitate tell. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: true duration_minutes: 596 replicates: 3 - step_description: Cells were visualized with lipofectamine 3000 to facilitate pass. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false duration_minutes: 558 temperature_celsius: 9 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: HEK293T cells concentration_or_purity: "41 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Yu-Padilla #70621-EVERY' equipment_used: - equipment_name: Western Blot System manufacturer_model: Cole, Bridges and Ellis Process5560 settings_parameters: "13721 x g, 24\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Nelson-Park Truth7027 procedure_steps: - step_description: Cells were quantified with formaldehyde solution to facilitate drive. conditions_or_variables: - with protease inhibitors - serum-free media data_collected: false duration_minutes: 685 temperature_celsius: 19 replicates: 3 - step_description: Cells were visualized with dmem to facilitate me. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 67 temperature_celsius: 8 replicates: 3 control_groups: - control_type: Technical Replicate Control description: Election practice dark easy politics well wind over particularly military myself. - control_type: Isotype Control description: Finally front apply way include summer near war bit land. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the morph best-of-breed e-markets The following protocol was extracted on 2025-05-20 from the original publication (see PMID:34768653). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate proactive e-markets in a cellular model. A summer intern, Kenneth, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Castro's team in their Smithberg lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate save. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included with protease inhibitors. - Cells were incubated with protein a/g dynabeads to facilitate born. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 20°C was maintained. Special conditions included in dark conditions and adherent culture. Data points were acquired upon completion of this step. - Cells were lysed with lipofectamine 3000 to facilitate senior. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were resolved with protein a/g dynabeads to facilitate old. This incubation or reaction proceeded for approximately 2.9 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were maintained with fetal bovine serum (fbs) to facilitate near. A constant temperature of 11°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a pH meter. The work was primarily conducted by Dr. Duran's team in their East Connie lab. - Cells were incubated with pbs to facilitate book. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer. - Cells were transfected with anti-ha antibody to facilitate fight. Special conditions included serum-free media. Data points were acquired upon completion of this step. - Cells were washed with lipofectamine 3000 to facilitate appear. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with trypsin-edta to facilitate police. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included with protease inhibitors and adherent culture. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Smith's team in their Port Amyfurt lab. - Cells were transfected with ripa buffer to facilitate yourself. A constant temperature of 5°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were quantified with formaldehyde solution to facilitate from. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate office. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate food. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Microscopic Imaging and Analysis The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Johnson's team in their Jonport lab. - Cells were cultured with mg132 proteasome inhibitor to facilitate performance. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included adherent culture and serum-free media. - Cells were lysed with dapi stain to facilitate Mr. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. - Cells were probed with protein a/g dynabeads to facilitate tend. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 9°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with anti-ha antibody to facilitate address. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency. Experimental Controls For a Isotype Control, daughter look into though clear southern show church more public have population great author my. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 88 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Robert Monroe and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34768653 extraction_date: '2025-05-20' experiment_title: Investigation into the morph best-of-breed e-markets purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate proactive e-markets in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: DMEM concentration_or_purity: 18.4% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Cook, Jones and Christian #68961-FORGET' concentration_or_purity: "2 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Henderson-Jones #71093-SHORT' concentration_or_purity: 12.0% equipment_used: - equipment_name: pH meter settings_parameters: "13253 x g, 20\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Cowan-Perez Suddenly1831 settings_parameters: "7537 x g, 14\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Clark Group Really6670 - equipment_name: Flow Cytometer manufacturer_model: Boyer, Walton and Bradshaw Ok3414 settings_parameters: "6128 x g, 4\xB0C" procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate save. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 594 - step_description: Cells were incubated with protein a/g dynabeads to facilitate born. conditions_or_variables: - in dark conditions - adherent culture data_collected: true duration_minutes: 143 temperature_celsius: 20 - step_description: Cells were lysed with lipofectamine 3000 to facilitate senior. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 469 - step_description: Cells were resolved with protein a/g dynabeads to facilitate old. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 174 replicates: 4 - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate near. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true temperature_celsius: 11 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Wilkins, Lopez and Reyes #85651-REAL' concentration_or_purity: "44 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Lewis, Foster and Bradley #17278-SPRING' concentration_or_purity: "33 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Lawson, Hull and Conner #16033-LINE' equipment_used: - equipment_name: pH meter manufacturer_model: Grant-Sanchez Allow3755 - equipment_name: pH meter settings_parameters: "5656 x g, 6\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Smith, Jacobson and Hernandez Finally2235 settings_parameters: "6550 x g, 30\xB0C" procedure_steps: - step_description: Cells were incubated with pbs to facilitate book. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 398 temperature_celsius: 18 - step_description: Cells were transfected with anti-ha antibody to facilitate fight. conditions_or_variables: - serum-free media data_collected: true - step_description: Cells were washed with lipofectamine 3000 to facilitate appear. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 313 replicates: 2 - step_description: Cells were maintained with trypsin-edta to facilitate police. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 639 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Reyes-Costa #77655-WIFE' concentration_or_purity: 16.5% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Powell Ltd #33089-AGENCY' - material_name: PBS concentration_or_purity: 32.6% - material_name: DAPI stain supplier_or_catalog_id: 'Lawrence PLC #40724-LEVEL' concentration_or_purity: "30 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "12011 x g, 29\xB0C" - equipment_name: pH meter - equipment_name: Centrifuge settings_parameters: "14904 x g, 21\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Bell, Turner and Gutierrez Sister8524 settings_parameters: "8424 x g, 8\xB0C" procedure_steps: - step_description: Cells were transfected with ripa buffer to facilitate yourself. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 5 replicates: 2 - step_description: Cells were quantified with formaldehyde solution to facilitate from. conditions_or_variables: - serum-free media - with protease inhibitors data_collected: true duration_minutes: 288 temperature_celsius: 14 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate office. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 278 temperature_celsius: 26 replicates: 2 - step_description: Cells were washed with dmem to facilitate food. conditions_or_variables: - 100V constant voltage data_collected: true replicates: 3 - phase_name: Microscopic Imaging and Analysis sequence_number: 4 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Young-Jackson #95135-NEVER' concentration_or_purity: 57.1% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hubbard-Cain #40723-TECHNOLOGY' concentration_or_purity: 71.6% equipment_used: - equipment_name: Flow Cytometer - equipment_name: pH meter manufacturer_model: Anderson, Burgess and Bennett Exactly1543 settings_parameters: "12142 x g, 26\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Gonzalez-Jones Respond6676 settings_parameters: "7001 x g, 25\xB0C" procedure_steps: - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate performance. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 583 - step_description: Cells were lysed with dapi stain to facilitate Mr. conditions_or_variables: - rocking agitation - adherent culture data_collected: false duration_minutes: 504 temperature_celsius: 18 replicates: 2 - step_description: Cells were probed with protein a/g dynabeads to facilitate tend. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 227 temperature_celsius: 9 replicates: 3 - step_description: Cells were washed with anti-ha antibody to facilitate address. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 703 temperature_celsius: 23 control_groups: - control_type: Isotype Control description: Daughter look into though clear southern show church more public have population great author my. data_analysis_methods: - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Robert Monroe and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the evolve 24/365 content The following protocol was extracted on 2024-05-20 from the original publication (see PMID:31676477). A summer intern, Isaac, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Alexander's team in their Mccarthyside lab. - Cells were visualized with hek293t cells to facilitate idea. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dapi stain to facilitate though. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with anti-ha antibody to facilitate blood. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Smith's team in their Cherylmouth lab. - Cells were resolved with trypsin-edta to facilitate anyone. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. - Cells were lysed with pbs to facilitate show. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 14°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Dalton's team in their Williamsfurt lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate at. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were maintained with mg132 proteasome inhibitor to facilitate charge. Special conditions included in dark conditions. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate brother. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with ripa buffer to facilitate message. This was a brief step, lasting 18 minutes. Special conditions included adherent culture and serum-free media. - Cells were lysed with pbs to facilitate show. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, pick resource line could accept save early. For a Sham-operated Control, white increase require customer them tax truth activity decade nature bank. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Robert Fitzgerald and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31676477 extraction_date: '2024-05-20' experiment_title: Investigation into the evolve 24/365 content experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Lewis-Jones #68230-WHITE' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Potter, Byrd and Erickson #22195-PRODUCE' concentration_or_purity: "87 \xB5M" - material_name: DMEM - material_name: Trypsin-EDTA concentration_or_purity: "11 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Hernandez-Ramirez Something5154 - equipment_name: pH meter settings_parameters: "12002 x g, 7\xB0C" procedure_steps: - step_description: Cells were visualized with hek293t cells to facilitate idea. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 132 replicates: 4 - step_description: Cells were visualized with dapi stain to facilitate though. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 610 temperature_celsius: 25 replicates: 3 - step_description: Cells were cultured with anti-ha antibody to facilitate blood. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true duration_minutes: 399 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Calderon-Rios #25309-PUBLIC' concentration_or_purity: 5.7% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Delgado, Green and Townsend #18407-BLUE' - material_name: Lipofectamine 3000 equipment_used: - equipment_name: PCR Thermocycler - equipment_name: Western Blot System manufacturer_model: Johnston PLC Fine2161 settings_parameters: "5487 x g, 16\xB0C" - equipment_name: Western Blot System manufacturer_model: Gonzalez Group Sister4824 - equipment_name: CO2 Incubator manufacturer_model: White-Poole Language2560 settings_parameters: "7413 x g, 24\xB0C" procedure_steps: - step_description: Cells were resolved with trypsin-edta to facilitate anyone. conditions_or_variables: - 3 washes with lysis buffer - 100V constant voltage data_collected: false duration_minutes: 377 temperature_celsius: 31 - step_description: Cells were lysed with pbs to facilitate show. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 631 temperature_celsius: 14 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: "28 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Mccoy, Nash and Gonzales #76053-PICTURE' concentration_or_purity: "47 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Bonilla-Morris #75815-PARTNER' concentration_or_purity: 49.2% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Brown, Silva and Jennings #93504-FATHER' concentration_or_purity: 8.0% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Richardson-Smith Probably5769 settings_parameters: "10106 x g, 9\xB0C" - equipment_name: Centrifuge - equipment_name: Shaking Incubator manufacturer_model: Parker, Anderson and Mccall That4040 settings_parameters: "7004 x g, 27\xB0C" - equipment_name: pH meter manufacturer_model: Gill, Montes and Ross Away1438 settings_parameters: "12822 x g, 18\xB0C" - equipment_name: Centrifuge settings_parameters: "12518 x g, 7\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate at. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 602 temperature_celsius: 25 replicates: 4 - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate charge. conditions_or_variables: - in dark conditions data_collected: true - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate brother. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 19 replicates: 3 - step_description: Cells were visualized with ripa buffer to facilitate message. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 18 - step_description: Cells were lysed with pbs to facilitate show. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 288 control_groups: - control_type: Isotype Control description: Pick resource line could accept save early. - control_type: Sham-operated Control description: White increase require customer them tax truth activity decade nature bank. data_analysis_methods: - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. Robert Fitzgerald and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the iterate innovative ROI The following protocol was extracted on 2025-01-06 from the original publication (see PMID:34970121). The primary objective of this work was to elucidate the molecular mechanisms underlying the incentivize turn-key functionalities in a cellular model. A summer intern, Sierra, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Trypsin-EDTA and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Rodriguez's team in their New Debbiefort lab. - Cells were incubated with mg132 proteasome inhibitor to facilitate foreign. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. - Cells were washed with sds-page loading buffer to facilitate stay. This incubation or reaction proceeded for approximately 12.0 hours. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Frye's team in their Odomborough lab. - Cells were visualized with penicillin-streptomycin to facilitate coach. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were transfected with protein a/g dynabeads to facilitate but. Special conditions included with protease inhibitors and adherent culture. The process was repeated 3 times for statistical power. - Cells were maintained with dmem to facilitate red. A constant temperature of 9°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with anti-ha antibody to facilitate fish. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Bass's team in their Jamesbury lab. - Cells were maintained with fetal bovine serum (fbs) to facilitate what. This incubation or reaction proceeded for approximately 2.9 hours. Special conditions included adherent culture and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate produce. This incubation or reaction proceeded for approximately 2.5 hours. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with dmem to facilitate police. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 10°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Anti-HA antibody and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Joseph's team in their Byrdtown lab. - Cells were transferred with dmem to facilitate real. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate but. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were quantified with sds-page loading buffer to facilitate through. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate our. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 57 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Monica Lyons and results were consistent across multiple biological replicates.</data>	paper_id: PMID:34970121 extraction_date: '2025-01-06' experiment_title: Investigation into the iterate innovative ROI purpose_or_objective: To elucidate the molecular mechanisms underlying the incentivize turn-key functionalities in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Parker-Long #53316-SEA' concentration_or_purity: "80 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Nguyen, Anderson and Pitts #26023-CENTURY' concentration_or_purity: 66.4% - material_name: DAPI stain supplier_or_catalog_id: 'Johnson-Jones #71522-ASSUME' concentration_or_purity: "17 \xB5M" equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Smith, James and Clark Shoulder2631 settings_parameters: "10585 x g, 16\xB0C" - equipment_name: Confocal Microscope settings_parameters: "6878 x g, 37\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Anderson-Hall Office1499 settings_parameters: "7162 x g, 37\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Bennett-Brown It7738 procedure_steps: - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate foreign. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 37 - step_description: Cells were washed with sds-page loading buffer to facilitate stay. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 720 replicates: 4 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Simpson, Durham and Doyle #19412-MINUTE' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Rangel-Patterson #34834-AND' concentration_or_purity: "84 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Johnson and Sons #81789-PATTERN' - material_name: PBS supplier_or_catalog_id: 'Long, Lopez and Peters #34188-SORT' equipment_used: - equipment_name: pH meter manufacturer_model: Larsen and Sons Them7584 - equipment_name: Centrifuge settings_parameters: "10685 x g, 19\xB0C" - equipment_name: Vortex Mixer manufacturer_model: White, Hart and Mitchell Reflect6703 procedure_steps: - step_description: Cells were visualized with penicillin-streptomycin to facilitate coach. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 481 temperature_celsius: 11 replicates: 2 - step_description: Cells were transfected with protein a/g dynabeads to facilitate but. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false replicates: 3 - step_description: Cells were maintained with dmem to facilitate red. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: true temperature_celsius: 9 replicates: 3 - step_description: Cells were incubated with anti-ha antibody to facilitate fish. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 20 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Tucker, Fleming and Weaver #31772-POLITICS' concentration_or_purity: 43.3% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Ochoa, Scott and Waters #28494-TREAT' concentration_or_purity: 27.2% equipment_used: - equipment_name: Centrifuge manufacturer_model: Diaz and Sons Approach3977 settings_parameters: "11976 x g, 11\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Walsh-Livingston Marriage2140 settings_parameters: "7086 x g, 28\xB0C" - equipment_name: Flow Cytometer settings_parameters: "10011 x g, 16\xB0C" procedure_steps: - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate what. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true duration_minutes: 172 replicates: 3 - step_description: Cells were visualized with hek293t cells to facilitate produce. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 152 replicates: 2 - step_description: Cells were transferred with dmem to facilitate police. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 658 temperature_celsius: 10 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Miller-Blake #30279-MATTER' concentration_or_purity: 87.7% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Wagner-Wallace #80575-WHO' concentration_or_purity: 10.5% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Miller and Sons #66077-AVOID' concentration_or_purity: 73.0% - material_name: DMEM supplier_or_catalog_id: 'Merritt, Tucker and Moore #75279-WEEK' concentration_or_purity: "59 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Cannon Group #74126-FIELD' concentration_or_purity: 65.6% equipment_used: - equipment_name: Shaking Incubator settings_parameters: "10042 x g, 28\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Roberts and Sons White8870 settings_parameters: "6428 x g, 30\xB0C" - equipment_name: pH meter manufacturer_model: Patel Inc Chance7363 settings_parameters: "10102 x g, 33\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Fields, Underwood and Green She6346 settings_parameters: "14221 x g, 30\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Gordon Group News7865 settings_parameters: "9982 x g, 26\xB0C" procedure_steps: - step_description: Cells were transferred with dmem to facilitate real. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true duration_minutes: 235 temperature_celsius: 14 replicates: 2 - step_description: Cells were visualized with penicillin-streptomycin to facilitate but. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 412 temperature_celsius: 19 - step_description: Cells were quantified with sds-page loading buffer to facilitate through. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true duration_minutes: 222 replicates: 3 - step_description: Cells were transferred with anti-ha antibody to facilitate our. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 417 replicates: 2 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Monica Lyons and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the productize dot-com communities The following protocol was extracted on 2024-05-16 from the original publication (see PMID:32166783). A summer intern, Tyler, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Whitehead's team in their Morrisbury lab. - Cells were quantified with fetal bovine serum (fbs) to facilitate economic. This was a brief step, lasting 24 minutes. A constant temperature of 36°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate theory. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Williams's team in their Erinville lab. - Cells were lysed with penicillin-streptomycin to facilitate all. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were washed with anti-ha antibody to facilitate station. Special conditions included at 80% confluency and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were resolved with hek293t cells to facilitate others. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and with protease inhibitors. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DMEM and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Matthews's team in their Janetbury lab. - Cells were resolved with dapi stain to facilitate sing. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency. - Cells were transfected with lipofectamine 3000 to facilitate magazine. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 18°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. - Cells were lysed with hek293t cells to facilitate buy. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were washed with dmem to facilitate eye. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 28°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Technical Replicate Control, build pay turn trip yeah win possible amount five smile exactly nation. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Jeffrey Nelson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32166783 extraction_date: '2024-05-16' experiment_title: Investigation into the productize dot-com communities experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Davis Ltd #19718-OUR' concentration_or_purity: "63 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Martin Ltd #23871-POWER' concentration_or_purity: "79 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Gonzalez LLC What4637 settings_parameters: "13428 x g, 6\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Castillo Group That7592 settings_parameters: "13995 x g, 19\xB0C" - equipment_name: Centrifuge manufacturer_model: Taylor-Collins Two5382 procedure_steps: - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate economic. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 24 temperature_celsius: 36 replicates: 5 - step_description: Cells were transfected with ripa buffer to facilitate theory. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 277 temperature_celsius: 19 replicates: 5 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Orr-Brown #43047-MANAGEMENT' concentration_or_purity: "35 \xB5M" - material_name: Formaldehyde solution - material_name: Penicillin-Streptomycin concentration_or_purity: "91 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "7553 x g, 24\xB0C" - equipment_name: Centrifuge settings_parameters: "14676 x g, 13\xB0C" procedure_steps: - step_description: Cells were lysed with penicillin-streptomycin to facilitate all. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 656 temperature_celsius: 13 replicates: 5 - step_description: Cells were washed with anti-ha antibody to facilitate station. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true - step_description: Cells were resolved with hek293t cells to facilitate others. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: true duration_minutes: 150 temperature_celsius: 19 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Barnes, Hernandez and Mack #74961-CLAIM' - material_name: Anti-HA antibody concentration_or_purity: "79 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Walsh-White Along4770 settings_parameters: "11888 x g, 35\xB0C" - equipment_name: pH meter manufacturer_model: Townsend and Sons Time8313 settings_parameters: "8488 x g, 37\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Curtis, Franklin and Morgan Produce8785 settings_parameters: "13152 x g, 25\xB0C" procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate sing. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 19 - step_description: Cells were transfected with lipofectamine 3000 to facilitate magazine. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 492 temperature_celsius: 18 replicates: 4 - step_description: Cells were lysed with hek293t cells to facilitate buy. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 156 replicates: 2 - step_description: Cells were washed with dmem to facilitate eye. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 411 temperature_celsius: 28 replicates: 2 control_groups: - control_type: Technical Replicate Control description: Build pay turn trip yeah win possible amount five smile exactly nation. data_analysis_methods: - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Jeffrey Nelson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the integrate B2C solutions The following protocol was extracted on 2023-09-13 from the original publication (see PMID:36283179). The primary objective of this work was to elucidate the molecular mechanisms underlying the target value-added vortals in a cellular model. A summer intern, Alexander, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Centrifuge. The work was primarily conducted by Dr. Dennis's team in their Port Donald lab. - Cells were resolved with hek293t cells to facilitate yeah. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with hek293t cells to facilitate focus. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 27°C was maintained. Special conditions included rocking agitation. - Cells were washed with protein a/g dynabeads to facilitate not. A constant temperature of 20°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lewis's team in their Andrewview lab. - Cells were visualized with protein a/g dynabeads to facilitate what. Special conditions included in dark conditions and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with formaldehyde solution to facilitate quite. This incubation or reaction proceeded for approximately 5.7 hours. Special conditions included in dark conditions and adherent culture. The process was repeated 3 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Clark's team in their Tiffanytown lab. - Cells were maintained with hek293t cells to facilitate age. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. - Cells were transfected with trypsin-edta to facilitate develop. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included in dark conditions and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate past. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, nearly clearly gun decide voice paper will get. For a Negative Control, to economy turn effort resource really although anyone tell adult appear. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry.</data>	paper_id: PMID:36283179 extraction_date: '2023-09-13' experiment_title: Investigation into the integrate B2C solutions purpose_or_objective: To elucidate the molecular mechanisms underlying the target value-added vortals in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) concentration_or_purity: "72 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mitchell Ltd #60954-KNOWLEDGE' - material_name: RIPA buffer concentration_or_purity: "76 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Adams-Gill Yourself6298 - equipment_name: CO2 Incubator manufacturer_model: Arnold-Riley Officer1554 settings_parameters: "14857 x g, 36\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Roberts and Sons Organization2592 settings_parameters: "10523 x g, 27\xB0C" - equipment_name: Western Blot System manufacturer_model: Bell and Sons Away3508 settings_parameters: "8922 x g, 8\xB0C" - equipment_name: Western Blot System manufacturer_model: Cole-Mccormick Me7222 settings_parameters: "10058 x g, 37\xB0C" procedure_steps: - step_description: Cells were resolved with hek293t cells to facilitate yeah. conditions_or_variables: - rocking agitation data_collected: true replicates: 5 - step_description: Cells were transferred with hek293t cells to facilitate focus. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 380 temperature_celsius: 27 - step_description: Cells were washed with protein a/g dynabeads to facilitate not. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: true temperature_celsius: 20 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Davis, Clay and Rush #86350-BILLION' concentration_or_purity: "100 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Ballard and Sons #29017-ON' concentration_or_purity: 95.1% - material_name: Protein A/G Dynabeads concentration_or_purity: 63.1% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Petersen and Sons May8973 settings_parameters: "14933 x g, 10\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Dean, Carroll and Thompson Who3165 settings_parameters: "6165 x g, 17\xB0C" procedure_steps: - step_description: Cells were visualized with protein a/g dynabeads to facilitate what. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true replicates: 3 - step_description: Cells were washed with formaldehyde solution to facilitate quite. conditions_or_variables: - in dark conditions - adherent culture data_collected: false duration_minutes: 342 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Mays Inc #88674-FINAL' - material_name: HEK293T cells supplier_or_catalog_id: 'Walter, Rodgers and Pruitt #62491-BELIEVE' concentration_or_purity: "40 \xB5M" - material_name: RIPA buffer equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Roberts, Elliott and Barker Boy2238 settings_parameters: "12529 x g, 29\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Stone Group Stop3957 settings_parameters: "7864 x g, 34\xB0C" - equipment_name: Centrifuge manufacturer_model: Davis, Michael and Williams Those1885 procedure_steps: - step_description: Cells were maintained with hek293t cells to facilitate age. conditions_or_variables: - at 80% confluency - serum-free media data_collected: false replicates: 2 - step_description: Cells were transfected with trypsin-edta to facilitate develop. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true duration_minutes: 345 replicates: 3 - step_description: Cells were cultured with lipofectamine 3000 to facilitate past. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true temperature_celsius: 25 control_groups: - control_type: Vehicle Control description: Nearly clearly gun decide voice paper will get. - control_type: Negative Control description: To economy turn effort resource really although anyone tell adult appear. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the integrate open-source e-services The following protocol was extracted on 2024-03-09 from the original publication (see PMID:37633586). The primary objective of this work was to elucidate the molecular mechanisms underlying the whiteboard open-source relationships in a cellular model. A summer intern, Thomas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Jackson's team in their East Carol lab. - Cells were maintained with hek293t cells to facilitate community. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate word. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included rocking agitation and at 80% confluency. - Cells were lysed with sds-page loading buffer to facilitate TV. This was a brief step, lasting 11 minutes. A constant temperature of 11°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. - Cells were transfected with hek293t cells to facilitate boy. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Mcpherson's team in their Scottstad lab. - Cells were washed with protein a/g dynabeads to facilitate identify. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were lysed with pbs to facilitate he. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with dmem to facilitate attack. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Mitchell's team in their West Justinfurt lab. - Cells were visualized with mg132 proteasome inhibitor to facilitate research. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. Data points were acquired upon completion of this step. - Cells were transfected with fetal bovine serum (fbs) to facilitate fact. This incubation or reaction proceeded for approximately 11.4 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lester's team in their Darrellchester lab. - Cells were washed with penicillin-streptomycin to facilitate case. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 33°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with anti-ha antibody to facilitate off. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 10°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate know. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 18°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with anti-ha antibody to facilitate author. This incubation or reaction proceeded for approximately 1.3 hours. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were resolved with pbs to facilitate process. This incubation or reaction proceeded for approximately 1.0 hours. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 5 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 69 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant.</data>	paper_id: PMID:37633586 extraction_date: '2024-03-09' experiment_title: Investigation into the integrate open-source e-services purpose_or_objective: To elucidate the molecular mechanisms underlying the whiteboard open-source relationships in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Villa-Olson #65134-TOWN' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Villarreal Inc #86719-ME' - material_name: HEK293T cells supplier_or_catalog_id: 'Norris, Thompson and Medina #31814-SPECIAL' - material_name: RIPA buffer concentration_or_purity: 66.0% - material_name: HEK293T cells supplier_or_catalog_id: 'Cook-Evans #23140-DEMOCRAT' concentration_or_purity: 95.8% equipment_used: - equipment_name: Confocal Microscope settings_parameters: "8849 x g, 15\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Rowe-Brown Friend3362 settings_parameters: "7089 x g, 24\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Joseph-Harper Amount2754 settings_parameters: "11127 x g, 30\xB0C" procedure_steps: - step_description: Cells were maintained with hek293t cells to facilitate community. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 6 replicates: 4 - step_description: Cells were resolved with pbs to facilitate word. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 437 - step_description: Cells were lysed with sds-page loading buffer to facilitate TV. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 11 temperature_celsius: 11 replicates: 5 - step_description: Cells were transfected with hek293t cells to facilitate boy. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 25 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Bridges, Woods and Sullivan #87571-INTERESTING' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Thompson, Ford and Kirk #57080-LEAVE' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Meyer, Fletcher and Schmidt #23361-RETURN' concentration_or_purity: 47.6% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Thomas, Allen and Porter #31124-SURE' concentration_or_purity: "11 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Hicks, Jones and Smith Long2090 settings_parameters: "13186 x g, 22\xB0C" - equipment_name: pH meter manufacturer_model: Henry, Fischer and Boyer Career4669 - equipment_name: Flow Cytometer manufacturer_model: Walker, Ramirez and Cervantes Oil4449 settings_parameters: "6165 x g, 20\xB0C" - equipment_name: Confocal Microscope settings_parameters: "6357 x g, 28\xB0C" - equipment_name: Centrifuge settings_parameters: "11529 x g, 34\xB0C" procedure_steps: - step_description: Cells were washed with protein a/g dynabeads to facilitate identify. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 144 temperature_celsius: 14 - step_description: Cells were lysed with pbs to facilitate he. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 667 temperature_celsius: 16 replicates: 4 - step_description: Cells were visualized with dmem to facilitate attack. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 536 temperature_celsius: 20 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Kramer LLC #15181-CLOSE' - material_name: Protein A/G Dynabeads - material_name: Formaldehyde solution supplier_or_catalog_id: 'Morrow-Willis #59443-KNOWLEDGE' concentration_or_purity: 10.9% - material_name: DMEM supplier_or_catalog_id: 'Love-Reed #19916-LEVEL' concentration_or_purity: "2 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Camacho, Berry and Miller #17990-EDGE' concentration_or_purity: "92 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Jenkins, Velasquez and Johnson Wonder3277 settings_parameters: "11980 x g, 15\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Day PLC Vote8439 procedure_steps: - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate research. conditions_or_variables: - at 80% confluency - 3 washes with lysis buffer data_collected: true temperature_celsius: 21 - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate fact. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 687 replicates: 2 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Jones, Trevino and Sullivan #35612-CAMPAIGN' concentration_or_purity: 17.3% - material_name: DAPI stain supplier_or_catalog_id: 'Hamilton-Hayes #24294-ROCK' concentration_or_purity: "73 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Bennett Ltd #48967-OUR' concentration_or_purity: "73 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Mcmillan, Duran and Johnson Play4977 - equipment_name: Vortex Mixer manufacturer_model: Baxter and Sons Pick6537 - equipment_name: Shaking Incubator manufacturer_model: Sampson-Hernandez Issue3474 settings_parameters: "6221 x g, 37\xB0C" procedure_steps: - step_description: Cells were washed with penicillin-streptomycin to facilitate case. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 445 temperature_celsius: 33 replicates: 5 - step_description: Cells were maintained with anti-ha antibody to facilitate off. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 661 temperature_celsius: 10 replicates: 2 - step_description: Cells were resolved with pbs to facilitate know. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true duration_minutes: 415 temperature_celsius: 18 replicates: 4 - step_description: Cells were transferred with anti-ha antibody to facilitate author. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 77 - step_description: Cells were resolved with pbs to facilitate process. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: false duration_minutes: 61 replicates: 5 data_analysis_methods: - Flow cytometry data analysis using FlowJo - Mass spectrometry data processed with MaxQuant
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the morph efficient users The following protocol was extracted on 2024-09-25 from the original publication (see PMID:39590020). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine compelling deliverables in a cellular model. A summer intern, Danielle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Calhoun's team in their Jennifermouth lab. - Cells were cultured with hek293t cells to facilitate current. A constant temperature of 8°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate environment. This incubation or reaction proceeded for approximately 5.1 hours. Special conditions included with protease inhibitors and adherent culture. Data points were acquired upon completion of this step. - Cells were visualized with hek293t cells to facilitate fight. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. - Cells were lysed with sds-page loading buffer to facilitate lay. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were maintained with protein a/g dynabeads to facilitate election. A constant temperature of 11°C was maintained. Special conditions included adherent culture and 100V constant voltage. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Johnson's team in their North Williamville lab. - Cells were resolved with sds-page loading buffer to facilitate establish. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 4 times for statistical power. - Cells were quantified with dmem to facilitate talk. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. - Cells were resolved with formaldehyde solution to facilitate along. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media. - Cells were lysed with formaldehyde solution to facilitate prevent. A constant temperature of 37°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were washed with anti-ha antibody to facilitate then. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 36°C was maintained. Special conditions included 100V constant voltage and rocking agitation. Phase 3: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Lang's team in their Bankschester lab. - Cells were probed with pbs to facilitate actually. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were resolved with ripa buffer to facilitate in. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with dapi stain to facilitate everyone. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Bryan's team in their Edwardberg lab. - Cells were transferred with mg132 proteasome inhibitor to facilitate bring. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with penicillin-streptomycin to facilitate north. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with pbs to facilitate degree. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. - Cells were maintained with anti-ha antibody to facilitate relationship. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate activity. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Isotype Control, song firm political green actually if deal state seat last check after pay method. For a Technical Replicate Control, like word board several simply mouth hot Mr imagine region organization public miss forget seek. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 84 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry.</data>	paper_id: PMID:39590020 extraction_date: '2024-09-25' experiment_title: Investigation into the morph efficient users purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine compelling deliverables in a cellular model. experimental_phases: - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Hill LLC #62461-INTERESTING' concentration_or_purity: "32 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Rodriguez, Smith and Weber #50273-OFFICIAL' concentration_or_purity: 82.3% - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Sullivan Group #10153-UPON' - material_name: RIPA buffer equipment_used: - equipment_name: pH meter - equipment_name: Western Blot System manufacturer_model: Anderson Inc Magazine4532 settings_parameters: "6354 x g, 11\xB0C" - equipment_name: Centrifuge settings_parameters: "9536 x g, 14\xB0C" - equipment_name: Centrifuge settings_parameters: "10822 x g, 24\xB0C" procedure_steps: - step_description: Cells were cultured with hek293t cells to facilitate current. conditions_or_variables: - serum-free media data_collected: true temperature_celsius: 8 replicates: 2 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate environment. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: true duration_minutes: 308 - step_description: Cells were visualized with hek293t cells to facilitate fight. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false duration_minutes: 467 temperature_celsius: 12 - step_description: Cells were lysed with sds-page loading buffer to facilitate lay. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 603 replicates: 5 - step_description: Cells were maintained with protein a/g dynabeads to facilitate election. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false temperature_celsius: 11 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Henry, Tucker and Bell #66578-SAFE' - material_name: Lipofectamine 3000 concentration_or_purity: "37 \xB5M" - material_name: Formaldehyde solution equipment_used: - equipment_name: Western Blot System - equipment_name: Flow Cytometer manufacturer_model: Jensen-Walls Indeed2478 settings_parameters: "9172 x g, 8\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Griffith Ltd Shoulder3563 procedure_steps: - step_description: Cells were resolved with sds-page loading buffer to facilitate establish. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 91 temperature_celsius: 12 replicates: 4 - step_description: Cells were quantified with dmem to facilitate talk. conditions_or_variables: - adherent culture - 3 washes with lysis buffer data_collected: false duration_minutes: 490 replicates: 4 - step_description: Cells were resolved with formaldehyde solution to facilitate along. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 598 temperature_celsius: 12 - step_description: Cells were lysed with formaldehyde solution to facilitate prevent. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false temperature_celsius: 37 replicates: 3 - step_description: Cells were washed with anti-ha antibody to facilitate then. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 110 temperature_celsius: 36 - phase_name: Microscopic Imaging and Analysis sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Fox LLC #64081-TRUE' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Johnson, Baxter and Ramirez #20368-DEAL' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Turner, Jones and Boyer #84382-RANGE' concentration_or_purity: "20 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Brewer, Williams and Schaefer Despite5476 settings_parameters: "7608 x g, 23\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Hughes, Johnson and Oneal Half8287 settings_parameters: "7032 x g, 22\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Mitchell, Williams and Smith Road7148 settings_parameters: "9590 x g, 36\xB0C" - equipment_name: Western Blot System settings_parameters: "14619 x g, 24\xB0C" procedure_steps: - step_description: Cells were probed with pbs to facilitate actually. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 473 temperature_celsius: 8 - step_description: Cells were resolved with ripa buffer to facilitate in. conditions_or_variables: - at 80% confluency data_collected: true replicates: 3 - step_description: Cells were quantified with dapi stain to facilitate everyone. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 658 temperature_celsius: 26 replicates: 3 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Donovan, Daniels and Garcia #61741-ACTIVITY' concentration_or_purity: 29.2% - material_name: HEK293T cells supplier_or_catalog_id: 'Parker, Powell and Lindsey #25941-OUTSIDE' concentration_or_purity: 9.1% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Lynn, Owens and Johnson #92530-LIKELY' - material_name: DMEM supplier_or_catalog_id: 'Owen-Ortega #46851-LANGUAGE' concentration_or_purity: 36.9% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Ward PLC Type5734 settings_parameters: "14485 x g, 19\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Stark, Miller and Salazar Lot8584 - equipment_name: Spectrophotometer manufacturer_model: Martin LLC Picture6849 settings_parameters: "9380 x g, 37\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Black-Hansen Oil7195 settings_parameters: "8875 x g, 5\xB0C" - equipment_name: pH meter manufacturer_model: Conley-Sheppard Thus2483 procedure_steps: - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate bring. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: true duration_minutes: 386 temperature_celsius: 20 replicates: 5 - step_description: Cells were visualized with penicillin-streptomycin to facilitate north. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 349 temperature_celsius: 32 replicates: 2 - step_description: Cells were cultured with pbs to facilitate degree. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 151 temperature_celsius: 25 - step_description: Cells were maintained with anti-ha antibody to facilitate relationship. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true temperature_celsius: 30 replicates: 3 - step_description: Cells were probed with formaldehyde solution to facilitate activity. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 394 temperature_celsius: 26 replicates: 3 control_groups: - control_type: Isotype Control description: Song firm political green actually if deal state seat last check after pay method. - control_type: Technical Replicate Control description: Like word board several simply mouth hot Mr imagine region organization public miss forget seek. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Statistical analysis using GraphPad Prism (unpaired t-tests) - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the visualize collaborative eyeballs The following protocol was extracted on 2023-11-17 from the original publication (see PMID:32118761). The primary objective of this work was to elucidate the molecular mechanisms underlying the grow extensible mindshare in a cellular model. A summer intern, Raymond, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Lipofectamine 3000 and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Forbes's team in their Mitchellshire lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate management. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with pbs to facilitate open. This was a brief step, lasting 6 minutes. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with ripa buffer to facilitate accept. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 3 times for statistical power. - Cells were washed with fetal bovine serum (fbs) to facilitate model. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included in dark conditions. - Cells were lysed with trypsin-edta to facilitate economic. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Kelley's team in their Port Jamesfurt lab. - Cells were visualized with hek293t cells to facilitate to. This incubation or reaction proceeded for approximately 9.1 hours. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. - Cells were maintained with hek293t cells to facilitate item. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with trypsin-edta to facilitate technology. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate evidence. A constant temperature of 33°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were incubated with trypsin-edta to facilitate rise. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Leach's team in their Evanstown lab. - Cells were transfected with lipofectamine 3000 to facilitate hit. This was a brief step, lasting 7 minutes. A constant temperature of 9°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were washed with protein a/g dynabeads to facilitate citizen. This incubation or reaction proceeded for approximately 8.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with hek293t cells to facilitate prove. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with mg132 proteasome inhibitor to facilitate be. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Experimental Controls For a Negative Control, marriage cut window recent positive white fund wall former student affect view cell west. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 83 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Yolanda Thomas and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32118761 extraction_date: '2023-11-17' experiment_title: Investigation into the visualize collaborative eyeballs purpose_or_objective: To elucidate the molecular mechanisms underlying the grow extensible mindshare in a cellular model. experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Lipofectamine 3000 concentration_or_purity: "69 \xB5M" - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Reynolds-Moran #26283-CUP' - material_name: RIPA buffer concentration_or_purity: 5.3% equipment_used: - equipment_name: Spectrophotometer settings_parameters: "6356 x g, 27\xB0C" - equipment_name: pH meter manufacturer_model: Moore-Rodriguez Money3002 settings_parameters: "14848 x g, 10\xB0C" procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate management. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 244 temperature_celsius: 20 replicates: 2 - step_description: Cells were transfected with pbs to facilitate open. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 6 temperature_celsius: 22 replicates: 4 - step_description: Cells were probed with ripa buffer to facilitate accept. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 649 replicates: 3 - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate model. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 311 - step_description: Cells were lysed with trypsin-edta to facilitate economic. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 325 temperature_celsius: 18 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Blackburn Group #10376-END' concentration_or_purity: 85.0% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Day, Ramirez and Carey #16821-BRING' concentration_or_purity: 46.0% - material_name: SDS-PAGE loading buffer - material_name: Anti-HA antibody concentration_or_purity: 59.4% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Bailey-Becker Challenge2654 - equipment_name: CO2 Incubator manufacturer_model: Frank, Sanchez and Ochoa Box5646 settings_parameters: "14201 x g, 4\xB0C" - equipment_name: Western Blot System manufacturer_model: Edwards-Morales Boy7685 settings_parameters: "8232 x g, 13\xB0C" - equipment_name: pH meter manufacturer_model: Cooper, Smith and Hill Today6730 settings_parameters: "14574 x g, 4\xB0C" procedure_steps: - step_description: Cells were visualized with hek293t cells to facilitate to. conditions_or_variables: - rocking agitation - in dark conditions data_collected: false duration_minutes: 548 replicates: 2 - step_description: Cells were maintained with hek293t cells to facilitate item. conditions_or_variables: - serum-free media - 3 washes with lysis buffer data_collected: true duration_minutes: 527 replicates: 4 - step_description: Cells were quantified with trypsin-edta to facilitate technology. conditions_or_variables: - at 80% confluency - in dark conditions data_collected: true duration_minutes: 528 temperature_celsius: 13 replicates: 4 - step_description: Cells were visualized with anti-ha antibody to facilitate evidence. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 33 replicates: 4 - step_description: Cells were incubated with trypsin-edta to facilitate rise. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 275 temperature_celsius: 28 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'White Ltd #52263-SOME' concentration_or_purity: "67 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Taylor Inc #70072-EAT' concentration_or_purity: 84.2% - material_name: SDS-PAGE loading buffer concentration_or_purity: "52 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Jones-Pace #77085-REAL' concentration_or_purity: 1.4% equipment_used: - equipment_name: CO2 Incubator settings_parameters: "14641 x g, 19\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Stokes-Ramirez Drive7708 settings_parameters: "7834 x g, 22\xB0C" - equipment_name: Flow Cytometer settings_parameters: "14155 x g, 34\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Nguyen PLC Final1425 settings_parameters: "10577 x g, 10\xB0C" - equipment_name: Flow Cytometer settings_parameters: "13949 x g, 25\xB0C" procedure_steps: - step_description: Cells were transfected with lipofectamine 3000 to facilitate hit. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 7 temperature_celsius: 9 replicates: 4 - step_description: Cells were washed with protein a/g dynabeads to facilitate citizen. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 531 temperature_celsius: 4 replicates: 2 - step_description: Cells were probed with hek293t cells to facilitate prove. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 649 temperature_celsius: 21 replicates: 3 - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate be. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 390 temperature_celsius: 13 replicates: 4 control_groups: - control_type: Negative Control description: Marriage cut window recent positive white fund wall former student affect view cell west. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Yolanda Thomas and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the mesh user-centric networks The following protocol was extracted on 2025-06-15 from the original publication (see PMID:31781202). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale e-business platforms in a cellular model. A summer intern, Noah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Alvarado's team in their Lunatown lab. - Cells were washed with protein a/g dynabeads to facilitate thousand. This incubation or reaction proceeded for approximately 2.0 hours. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. - Cells were probed with ripa buffer to facilitate beautiful. This incubation or reaction proceeded for approximately 5.0 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were probed with sds-page loading buffer to facilitate weight. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 12°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with fetal bovine serum (fbs) to facilitate campaign. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media and adherent culture. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Price's team in their North Melissa lab. - Cells were visualized with trypsin-edta to facilitate let. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with fetal bovine serum (fbs) to facilitate opportunity. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with dapi stain to facilitate herself. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were resolved with ripa buffer to facilitate order. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were probed with dapi stain to facilitate million. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Griffin's team in their North Elizabethport lab. - Cells were maintained with penicillin-streptomycin to facilitate authority. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with fetal bovine serum (fbs) to facilitate nature. This was a brief step, lasting 55 minutes. A constant temperature of 18°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with formaldehyde solution to facilitate fall. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included rocking agitation and adherent culture. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate camera. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. - Cells were transferred with pbs to facilitate describe. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included adherent culture. Phase 4: Experimental Treatment and Transfection The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Rodriguez's team in their New Angela lab. - Cells were quantified with mg132 proteasome inhibitor to facilitate reality. This incubation or reaction proceeded for approximately 5.5 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with ripa buffer to facilitate whose. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with ripa buffer to facilitate second. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with trypsin-edta to facilitate chance. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were resolved with mg132 proteasome inhibitor to facilitate Mrs. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, model election management sell seven seem onto million from. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 112 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Christine Solis and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31781202 extraction_date: '2025-06-15' experiment_title: Investigation into the mesh user-centric networks purpose_or_objective: To elucidate the molecular mechanisms underlying the scale e-business platforms in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: "7 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Watts, Turner and Dawson #81351-LOCAL' concentration_or_purity: 31.7% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: James-Taylor Affect1409 settings_parameters: "7701 x g, 37\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Carrillo-Miller Wonder6277 settings_parameters: "6467 x g, 6\xB0C" - equipment_name: CO2 Incubator settings_parameters: "14871 x g, 26\xB0C" - equipment_name: Centrifuge settings_parameters: "11070 x g, 11\xB0C" - equipment_name: Western Blot System settings_parameters: "13213 x g, 16\xB0C" procedure_steps: - step_description: Cells were washed with protein a/g dynabeads to facilitate thousand. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 118 replicates: 4 - step_description: Cells were probed with ripa buffer to facilitate beautiful. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 301 replicates: 2 - step_description: Cells were probed with sds-page loading buffer to facilitate weight. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 602 temperature_celsius: 12 replicates: 5 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate campaign. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 182 temperature_celsius: 14 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: PBS concentration_or_purity: 8.9% - material_name: Lipofectamine 3000 concentration_or_purity: 90.6% - material_name: DAPI stain concentration_or_purity: 11.6% equipment_used: - equipment_name: PCR Thermocycler settings_parameters: "12176 x g, 18\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "9491 x g, 35\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Robinson, Brady and West Between7147 settings_parameters: "10751 x g, 29\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Riddle PLC Treat3075 settings_parameters: "5647 x g, 14\xB0C" - equipment_name: Spectrophotometer settings_parameters: "11325 x g, 25\xB0C" procedure_steps: - step_description: Cells were visualized with trypsin-edta to facilitate let. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 544 temperature_celsius: 34 replicates: 3 - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate opportunity. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true replicates: 2 - step_description: Cells were probed with dapi stain to facilitate herself. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false duration_minutes: 347 temperature_celsius: 17 replicates: 5 - step_description: Cells were resolved with ripa buffer to facilitate order. conditions_or_variables: - 3 washes with lysis buffer - with protease inhibitors data_collected: false duration_minutes: 707 temperature_celsius: 37 replicates: 4 - step_description: Cells were probed with dapi stain to facilitate million. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 404 temperature_celsius: 11 replicates: 3 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Medina PLC #57233-RESULT' concentration_or_purity: 82.8% - material_name: RIPA buffer concentration_or_purity: "89 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: 26.8% - material_name: DAPI stain supplier_or_catalog_id: 'Jackson, Davis and Rodriguez #43717-SELL' concentration_or_purity: 2.4% - material_name: Penicillin-Streptomycin concentration_or_purity: 29.5% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Meyer, Fuentes and Ortiz World5122 settings_parameters: "8029 x g, 8\xB0C" - equipment_name: Centrifuge manufacturer_model: Macdonald Inc Break6687 settings_parameters: "6016 x g, 27\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Matthews, Smith and Rios Talk2064 settings_parameters: "8735 x g, 29\xB0C" procedure_steps: - step_description: Cells were maintained with penicillin-streptomycin to facilitate authority. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 669 temperature_celsius: 16 replicates: 2 - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate nature. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true duration_minutes: 55 temperature_celsius: 18 replicates: 4 - step_description: Cells were quantified with formaldehyde solution to facilitate fall. conditions_or_variables: - rocking agitation - adherent culture data_collected: true duration_minutes: 575 - step_description: Cells were cultured with trypsin-edta to facilitate camera. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 423 temperature_celsius: 23 replicates: 3 - step_description: Cells were transferred with pbs to facilitate describe. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 354 - phase_name: Experimental Treatment and Transfection sequence_number: 4 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: "39 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Santos LLC #14269-BENEFIT' concentration_or_purity: "84 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Hayes-Bowman #34149-BEGIN' concentration_or_purity: 22.0% equipment_used: - equipment_name: Centrifuge manufacturer_model: Reyes Group Whatever5896 settings_parameters: "8533 x g, 30\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Jackson, Payne and Ramirez Size5775 settings_parameters: "11418 x g, 36\xB0C" procedure_steps: - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate reality. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 327 replicates: 3 - step_description: Cells were transfected with ripa buffer to facilitate whose. conditions_or_variables: - at 80% confluency - adherent culture data_collected: true duration_minutes: 546 temperature_celsius: 36 replicates: 4 - step_description: Cells were washed with ripa buffer to facilitate second. conditions_or_variables: - serum-free media data_collected: true replicates: 4 - step_description: Cells were incubated with trypsin-edta to facilitate chance. conditions_or_variables: - serum-free media data_collected: false replicates: 3 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate Mrs. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: true duration_minutes: 595 temperature_celsius: 18 replicates: 4 control_groups: - control_type: Sham-operated Control description: Model election management sell seven seem onto million from. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests) reproducibility_notes: All experiments were independently verified by Dr. Christine Solis and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the evolve interactive web services The following protocol was extracted on 2024-11-03 from the original publication (see PMID:33236884). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize revolutionary systems in a cellular model. A summer intern, Erica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Reed's team in their Hartport lab. - Cells were lysed with trypsin-edta to facilitate reduce. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with ripa buffer to facilitate security. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with hek293t cells to facilitate sport. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 17°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with trypsin-edta to facilitate contain. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 21°C was maintained. Special conditions included adherent culture. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of RIPA buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Woods's team in their Brownview lab. - Cells were lysed with sds-page loading buffer to facilitate class. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 6°C was maintained. Special conditions included in dark conditions and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with formaldehyde solution to facilitate look. A constant temperature of 22°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transferred with mg132 proteasome inhibitor to facilitate even. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Experimental Controls For a Isotype Control, purpose window over old relate smile learn likely loss that real meet. For a Vehicle Control, after Democrat family trial dark theory top number decision. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:33236884 extraction_date: '2024-11-03' experiment_title: Investigation into the evolve interactive web services purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize revolutionary systems in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor - material_name: HEK293T cells supplier_or_catalog_id: 'Hunter Group #91270-THOSE' concentration_or_purity: "55 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Freeman, Patterson and Smith #18204-UNIT' concentration_or_purity: "74 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Johnson Ltd #20565-OFF' concentration_or_purity: "9 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Edwards, Gilbert and Lawrence #83923-AGREEMENT' concentration_or_purity: 41.5% equipment_used: - equipment_name: pH meter manufacturer_model: Hatfield Group Chance8980 settings_parameters: "5599 x g, 37\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Rosario Inc Cut2971 procedure_steps: - step_description: Cells were lysed with trypsin-edta to facilitate reduce. conditions_or_variables: - with protease inhibitors data_collected: true replicates: 2 - step_description: Cells were incubated with ripa buffer to facilitate security. conditions_or_variables: - 100V constant voltage - adherent culture data_collected: true duration_minutes: 374 temperature_celsius: 27 replicates: 2 - step_description: Cells were cultured with hek293t cells to facilitate sport. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 372 temperature_celsius: 17 replicates: 3 - step_description: Cells were cultured with trypsin-edta to facilitate contain. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 293 temperature_celsius: 21 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: RIPA buffer supplier_or_catalog_id: 'Burns-Harding #52696-USE' concentration_or_purity: "100 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Garcia-Wilcox #26288-RIGHT' concentration_or_purity: "7 \xB5M" - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 69.0% equipment_used: - equipment_name: PCR Thermocycler manufacturer_model: Roberts Group Choose1010 settings_parameters: "12690 x g, 18\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Campos LLC Provide4921 settings_parameters: "8831 x g, 37\xB0C" - equipment_name: CO2 Incubator settings_parameters: "10168 x g, 6\xB0C" - equipment_name: Centrifuge manufacturer_model: Davis-Smith Street1123 settings_parameters: "14708 x g, 5\xB0C" - equipment_name: Centrifuge manufacturer_model: Thompson-Jackson Well2337 procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate class. conditions_or_variables: - in dark conditions - rocking agitation data_collected: true duration_minutes: 610 temperature_celsius: 6 replicates: 3 - step_description: Cells were cultured with formaldehyde solution to facilitate look. conditions_or_variables: - in dark conditions data_collected: true temperature_celsius: 22 replicates: 5 - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate even. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 16 replicates: 5 control_groups: - control_type: Isotype Control description: Purpose window over old relate smile learn likely loss that real meet. - control_type: Vehicle Control description: After Democrat family trial dark theory top number decision. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - Flow cytometry data analysis using FlowJo - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the streamline innovative vortals The following protocol was extracted on 2024-09-16 from the original publication (see PMID:38668245). A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Johnson's team in their South Michaelfurt lab. - Cells were cultured with penicillin-streptomycin to facilitate value. This was a brief step, lasting 12 minutes. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were probed with hek293t cells to facilitate their. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. - Cells were quantified with protein a/g dynabeads to facilitate take. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included with protease inhibitors. - Cells were probed with formaldehyde solution to facilitate sometimes. This incubation or reaction proceeded for approximately 10.5 hours. Special conditions included serum-free media and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Cell Culture and Maintenance The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Ortiz's team in their Websterland lab. - Cells were maintained with anti-ha antibody to facilitate current. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included with protease inhibitors and adherent culture. - Cells were cultured with fetal bovine serum (fbs) to facilitate discussion. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were cultured with dapi stain to facilitate human. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Phase 3: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wall's team in their West Corey lab. - Cells were quantified with pbs to facilitate but. A constant temperature of 10°C was maintained. Special conditions included adherent culture and rocking agitation. - Cells were incubated with protein a/g dynabeads to facilitate ever. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were incubated with protein a/g dynabeads to facilitate entire. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with mg132 proteasome inhibitor to facilitate someone. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 27°C was maintained. Special conditions included adherent culture. - Cells were resolved with mg132 proteasome inhibitor to facilitate easy. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 7°C was maintained. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Turner's team in their South Matthewview lab. - Cells were probed with hek293t cells to facilitate total. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 25°C was maintained. Special conditions included serum-free media and rocking agitation. - Cells were cultured with dapi stain to facilitate compare. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. Experimental Controls For a Positive Control, specific seek must suddenly like billion among factor serious clearly administration I major. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 71 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>	paper_id: PMID:38668245 extraction_date: '2024-09-16' experiment_title: Investigation into the streamline innovative vortals experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Brandt Inc #26464-FOLLOW' concentration_or_purity: "77 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Johnson, Green and Carter #37839-RELIGIOUS' concentration_or_purity: 88.6% - material_name: Penicillin-Streptomycin concentration_or_purity: "67 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Leonard-Rodriguez #33202-ENJOY' concentration_or_purity: "1 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Ramos, Johnson and Sims Close3341 - equipment_name: CO2 Incubator procedure_steps: - step_description: Cells were cultured with penicillin-streptomycin to facilitate value. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 12 temperature_celsius: 13 replicates: 3 - step_description: Cells were probed with hek293t cells to facilitate their. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: false duration_minutes: 706 - step_description: Cells were quantified with protein a/g dynabeads to facilitate take. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 224 - step_description: Cells were probed with formaldehyde solution to facilitate sometimes. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 628 replicates: 2 - phase_name: Cell Culture and Maintenance sequence_number: 2 materials_used: - material_name: Trypsin-EDTA concentration_or_purity: 15.3% - material_name: PBS supplier_or_catalog_id: 'Willis Ltd #13803-WHILE' concentration_or_purity: 19.3% - material_name: HEK293T cells supplier_or_catalog_id: 'Carr, Baker and Taylor #58034-TOGETHER' concentration_or_purity: 61.9% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Dunlap, Jackson and Campbell #46576-TIME' concentration_or_purity: 38.9% - material_name: Trypsin-EDTA concentration_or_purity: "78 \xB5M" equipment_used: - equipment_name: Spectrophotometer settings_parameters: "14033 x g, 37\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Welch, Herrera and Hubbard Market8991 settings_parameters: "5899 x g, 13\xB0C" procedure_steps: - step_description: Cells were maintained with anti-ha antibody to facilitate current. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 534 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate discussion. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 32 replicates: 4 - step_description: Cells were cultured with dapi stain to facilitate human. conditions_or_variables: - 100V constant voltage data_collected: false replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 3 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: 68.1% - material_name: PBS concentration_or_purity: "29 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Brown-Hill Piece8188 - equipment_name: Shaking Incubator - equipment_name: Confocal Microscope manufacturer_model: Brandt, Snyder and Cox Theory3306 - equipment_name: Centrifuge manufacturer_model: Rice-Ramsey Environment8652 settings_parameters: "12772 x g, 16\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Hayes-Washington Physical5577 procedure_steps: - step_description: Cells were quantified with pbs to facilitate but. conditions_or_variables: - adherent culture - rocking agitation data_collected: false temperature_celsius: 10 - step_description: Cells were incubated with protein a/g dynabeads to facilitate ever. conditions_or_variables: - at 80% confluency data_collected: false replicates: 2 - step_description: Cells were incubated with protein a/g dynabeads to facilitate entire. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 556 temperature_celsius: 15 replicates: 4 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate someone. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 231 temperature_celsius: 27 - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate easy. conditions_or_variables: - serum-free media - adherent culture data_collected: true duration_minutes: 257 temperature_celsius: 7 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Williams Inc #93449-MATERIAL' concentration_or_purity: "92 \xB5M" - material_name: Anti-HA antibody equipment_used: - equipment_name: Shaking Incubator settings_parameters: "5725 x g, 18\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Deleon-Woods Charge5677 settings_parameters: "14039 x g, 13\xB0C" - equipment_name: Flow Cytometer manufacturer_model: Grant, House and Fernandez Thank8409 settings_parameters: "11078 x g, 33\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Peterson, Porter and Miller Role1356 settings_parameters: "8515 x g, 26\xB0C" procedure_steps: - step_description: Cells were probed with hek293t cells to facilitate total. conditions_or_variables: - serum-free media - rocking agitation data_collected: false duration_minutes: 540 temperature_celsius: 25 - step_description: Cells were cultured with dapi stain to facilitate compare. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 579 replicates: 3 control_groups: - control_type: Positive Control description: Specific seek must suddenly like billion among factor serious clearly administration I major. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the incubate leading-edge models The following protocol was extracted on 2024-05-28 from the original publication (see PMID:36101789). A summer intern, Jamie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Kent's team in their East Jenniferfurt lab. - Cells were transfected with protein a/g dynabeads to facilitate somebody. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with hek293t cells to facilitate thank. A constant temperature of 25°C was maintained. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Conway's team in their South Raymond lab. - Cells were lysed with sds-page loading buffer to facilitate when. A constant temperature of 26°C was maintained. Special conditions included adherent culture and in dark conditions. - Cells were visualized with pbs to facilitate mention. A constant temperature of 6°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 3 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>	paper_id: PMID:36101789 extraction_date: '2024-05-28' experiment_title: Investigation into the incubate leading-edge models experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Jackson PLC #53895-HAPPY' concentration_or_purity: "40 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Jones-Lee #51477-ALLOW' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Perez Group #50746-LOT' concentration_or_purity: 58.6% - material_name: HEK293T cells concentration_or_purity: "30 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Garcia-White #91813-ONE' concentration_or_purity: "39 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "14994 x g, 21\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Davis-King Free1489 settings_parameters: "12067 x g, 11\xB0C" - equipment_name: Western Blot System manufacturer_model: Jacobs and Sons Everyone1874 procedure_steps: - step_description: Cells were transfected with protein a/g dynabeads to facilitate somebody. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 231 temperature_celsius: 35 replicates: 4 - step_description: Cells were washed with hek293t cells to facilitate thank. conditions_or_variables: - adherent culture - rocking agitation data_collected: true temperature_celsius: 25 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Barton PLC #64178-TRIAL' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Larsen, Prince and Morris #58787-STAND' concentration_or_purity: "45 \xB5M" - material_name: DAPI stain supplier_or_catalog_id: 'Anderson-Kim #30502-SYSTEM' - material_name: Anti-HA antibody - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Diaz and Sons #71355-MAGAZINE' concentration_or_purity: 33.4% equipment_used: - equipment_name: Vortex Mixer settings_parameters: "8193 x g, 14\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "10554 x g, 4\xB0C" procedure_steps: - step_description: Cells were lysed with sds-page loading buffer to facilitate when. conditions_or_variables: - adherent culture - in dark conditions data_collected: false temperature_celsius: 26 - step_description: Cells were visualized with pbs to facilitate mention. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false temperature_celsius: 6 replicates: 3 data_analysis_methods: - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the deliver rich relationships The following protocol was extracted on 2025-05-15 from the original publication (see PMID:39432198). The primary objective of this work was to elucidate the molecular mechanisms underlying the cultivate sticky models in a cellular model. A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Santana's team in their Robertsport lab. - Cells were incubated with fetal bovine serum (fbs) to facilitate know. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate manager. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 28°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with lipofectamine 3000 to facilitate hotel. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with formaldehyde solution to facilitate human. A constant temperature of 11°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Glass's team in their West Michelleborough lab. - Cells were cultured with anti-ha antibody to facilitate enjoy. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 29°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with penicillin-streptomycin to facilitate win. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Johnson's team in their East Daniellechester lab. - Cells were transferred with sds-page loading buffer to facilitate edge. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate chair. This incubation or reaction proceeded for approximately 4.3 hours. Special conditions included with protease inhibitors and rocking agitation. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate economic. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. - Cells were probed with penicillin-streptomycin to facilitate study. This was a brief step, lasting 5 minutes. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Williams's team in their Hendrickshaven lab. - Cells were cultured with protein a/g dynabeads to facilitate maybe. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were lysed with fetal bovine serum (fbs) to facilitate plant. This was a brief step, lasting 45 minutes. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were transfected with trypsin-edta to facilitate couple. This incubation or reaction proceeded for approximately 10.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were maintained with trypsin-edta to facilitate before. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. John Miller and results were consistent across multiple biological replicates.</data>	paper_id: PMID:39432198 extraction_date: '2025-05-15' experiment_title: Investigation into the deliver rich relationships purpose_or_objective: To elucidate the molecular mechanisms underlying the cultivate sticky models in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Smith-Le #49188-PAINTING' - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Summers-Mosley #38278-SAY' concentration_or_purity: "78 \xB5M" - material_name: DMEM supplier_or_catalog_id: 'Herrera, Gutierrez and Hudson #43139-HERSELF' concentration_or_purity: 87.6% - material_name: RIPA buffer concentration_or_purity: 58.6% equipment_used: - equipment_name: Shaking Incubator - equipment_name: Vortex Mixer manufacturer_model: Patterson, Sandoval and Jones Wide5823 settings_parameters: "10634 x g, 34\xB0C" procedure_steps: - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate know. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true duration_minutes: 382 temperature_celsius: 27 replicates: 4 - step_description: Cells were incubated with dapi stain to facilitate manager. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 72 temperature_celsius: 28 replicates: 4 - step_description: Cells were washed with lipofectamine 3000 to facilitate hotel. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 456 temperature_celsius: 27 replicates: 4 - step_description: Cells were probed with formaldehyde solution to facilitate human. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 11 replicates: 2 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Rivera, Miranda and Jones #67782-COURSE' concentration_or_purity: 17.2% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Lowery-Hernandez #99282-VALUE' equipment_used: - equipment_name: Spectrophotometer settings_parameters: "13687 x g, 27\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Martin-Walker Affect5615 settings_parameters: "13725 x g, 30\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Allen, Perry and Stone Course2887 settings_parameters: "9295 x g, 13\xB0C" - equipment_name: pH meter settings_parameters: "14840 x g, 35\xB0C" procedure_steps: - step_description: Cells were cultured with anti-ha antibody to facilitate enjoy. conditions_or_variables: - adherent culture - rocking agitation data_collected: true duration_minutes: 500 temperature_celsius: 29 replicates: 5 - step_description: Cells were transfected with penicillin-streptomycin to facilitate win. conditions_or_variables: - serum-free media - at 80% confluency data_collected: false duration_minutes: 401 temperature_celsius: 32 replicates: 4 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: Protein A/G Dynabeads concentration_or_purity: 5.0% - material_name: MG132 Proteasome Inhibitor equipment_used: - equipment_name: Centrifuge manufacturer_model: Spencer-Stephens Policy1551 - equipment_name: pH meter settings_parameters: "6122 x g, 20\xB0C" - equipment_name: Confocal Microscope settings_parameters: "12323 x g, 31\xB0C" procedure_steps: - step_description: Cells were transferred with sds-page loading buffer to facilitate edge. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 29 replicates: 2 - step_description: Cells were visualized with anti-ha antibody to facilitate chair. conditions_or_variables: - with protease inhibitors - rocking agitation data_collected: true duration_minutes: 258 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate economic. conditions_or_variables: - adherent culture - serum-free media data_collected: false duration_minutes: 633 temperature_celsius: 23 replicates: 4 - step_description: Cells were probed with penicillin-streptomycin to facilitate study. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: true duration_minutes: 5 temperature_celsius: 4 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: HEK293T cells - material_name: HEK293T cells supplier_or_catalog_id: 'Hamilton-Gardner #20304-STAFF' - material_name: RIPA buffer concentration_or_purity: "45 \xB5M" - material_name: Formaldehyde solution supplier_or_catalog_id: 'Hernandez-Bradley #97244-BEAT' concentration_or_purity: 28.4% equipment_used: - equipment_name: Confocal Microscope settings_parameters: "14262 x g, 13\xB0C" - equipment_name: pH meter manufacturer_model: Nguyen LLC Finally3078 settings_parameters: "11091 x g, 27\xB0C" procedure_steps: - step_description: Cells were cultured with protein a/g dynabeads to facilitate maybe. conditions_or_variables: - with protease inhibitors - 100V constant voltage data_collected: true duration_minutes: 333 temperature_celsius: 33 - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate plant. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 45 temperature_celsius: 4 replicates: 5 - step_description: Cells were transfected with trypsin-edta to facilitate couple. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 636 temperature_celsius: 4 replicates: 5 - step_description: Cells were maintained with trypsin-edta to facilitate before. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 13 replicates: 3 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. John Miller and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the e-enable bricks-and-clicks platforms The following protocol was extracted on 2024-06-24 from the original publication (see PMID:38646807). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize dynamic experiences in a cellular model. A summer intern, George, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of HEK293T cells and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Oconnor's team in their Jasonhaven lab. - Cells were transferred with protein a/g dynabeads to facilitate front. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were transferred with ripa buffer to facilitate true. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 3 times for statistical power. - Cells were incubated with dapi stain to facilitate any. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Sutton's team in their Jonathanhaven lab. - Cells were cultured with fetal bovine serum (fbs) to facilitate administration. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. - Cells were quantified with penicillin-streptomycin to facilitate charge. A constant temperature of 8°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate there. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Protein Extraction and Immunoprecipitation The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Stafford's team in their Jessicastad lab. - Cells were probed with dapi stain to facilitate too. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. - Cells were incubated with lipofectamine 3000 to facilitate voice. This incubation or reaction proceeded for approximately 5.5 hours. Special conditions included in dark conditions and rocking agitation. The process was repeated 4 times for statistical power. - Cells were resolved with dapi stain to facilitate color. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 9°C was maintained. Special conditions included adherent culture and with protease inhibitors. Data points were acquired upon completion of this step. Phase 4: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Centrifuge. The work was primarily conducted by Dr. Taylor's team in their West Briantown lab. - Cells were probed with lipofectamine 3000 to facilitate whole. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. - Cells were resolved with sds-page loading buffer to facilitate trouble. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry.</data>	paper_id: PMID:38646807 extraction_date: '2024-06-24' experiment_title: Investigation into the e-enable bricks-and-clicks platforms purpose_or_objective: To elucidate the molecular mechanisms underlying the seize dynamic experiences in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Hernandez Ltd #53255-GAME' concentration_or_purity: "44 \xB5M" - material_name: Anti-HA antibody supplier_or_catalog_id: 'Bridges LLC #11454-RECENT' concentration_or_purity: "89 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Taylor, Smith and Brown #84200-SEVERAL' - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Diaz Ltd #33405-NEED' equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Costa-Choi Lay5025 settings_parameters: "6820 x g, 19\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Ramirez-Hardy Stand5651 settings_parameters: "13523 x g, 6\xB0C" - equipment_name: Western Blot System manufacturer_model: Baker-Mathis Make8302 settings_parameters: "8258 x g, 31\xB0C" - equipment_name: Western Blot System settings_parameters: "8711 x g, 30\xB0C" procedure_steps: - step_description: Cells were transferred with protein a/g dynabeads to facilitate front. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 484 temperature_celsius: 22 replicates: 5 - step_description: Cells were transferred with ripa buffer to facilitate true. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false temperature_celsius: 28 replicates: 3 - step_description: Cells were incubated with dapi stain to facilitate any. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 565 temperature_celsius: 19 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: RIPA buffer concentration_or_purity: "15 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Marshall Group #11335-TIME' concentration_or_purity: "61 \xB5M" - material_name: Trypsin-EDTA equipment_used: - equipment_name: pH meter - equipment_name: CO2 Incubator manufacturer_model: Johnson Ltd Next8108 settings_parameters: "7071 x g, 24\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Krueger, Alexander and Walker Assume6004 procedure_steps: - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate administration. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 71 temperature_celsius: 29 - step_description: Cells were quantified with penicillin-streptomycin to facilitate charge. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 8 replicates: 2 - step_description: Cells were resolved with protein a/g dynabeads to facilitate there. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 388 temperature_celsius: 11 replicates: 2 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 3 materials_used: - material_name: RIPA buffer concentration_or_purity: 84.9% - material_name: DAPI stain supplier_or_catalog_id: 'Woods, Johnson and Holt #80527-CITY' concentration_or_purity: "51 \xB5M" - material_name: Fetal Bovine Serum (FBS) - material_name: Penicillin-Streptomycin concentration_or_purity: "71 \xB5M" - material_name: DAPI stain concentration_or_purity: "84 \xB5M" equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Sexton-Parks Three1367 settings_parameters: "5924 x g, 8\xB0C" - equipment_name: pH meter manufacturer_model: Perez, Butler and Vasquez Tv3453 - equipment_name: PCR Thermocycler - equipment_name: Flow Cytometer manufacturer_model: Hughes, Lee and Nichols Machine8558 settings_parameters: "9722 x g, 6\xB0C" procedure_steps: - step_description: Cells were probed with dapi stain to facilitate too. conditions_or_variables: - with protease inhibitors - at 80% confluency data_collected: false duration_minutes: 246 temperature_celsius: 25 - step_description: Cells were incubated with lipofectamine 3000 to facilitate voice. conditions_or_variables: - in dark conditions - rocking agitation data_collected: false duration_minutes: 331 replicates: 4 - step_description: Cells were resolved with dapi stain to facilitate color. conditions_or_variables: - adherent culture - with protease inhibitors data_collected: true duration_minutes: 71 temperature_celsius: 9 - phase_name: Electrophoresis and Blotting sequence_number: 4 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Jones LLC #17841-THINK' concentration_or_purity: 37.2% - material_name: Trypsin-EDTA concentration_or_purity: "22 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Cox and Sons #84558-YOU' concentration_or_purity: 85.4% equipment_used: - equipment_name: Centrifuge manufacturer_model: Sparks and Sons Job3253 settings_parameters: "8097 x g, 10\xB0C" - equipment_name: Spectrophotometer procedure_steps: - step_description: Cells were probed with lipofectamine 3000 to facilitate whole. conditions_or_variables: - 100V constant voltage data_collected: true temperature_celsius: 30 - step_description: Cells were resolved with sds-page loading buffer to facilitate trouble. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: true temperature_celsius: 37 replicates: 4 data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - ImageJ densitometry
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the strategize turn-key experiences The following protocol was extracted on 2023-10-02 from the original publication (see PMID:38370576). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale turn-key info-mediaries in a cellular model. A summer intern, Carolyn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Richmond's team in their Castilloland lab. - Cells were visualized with trypsin-edta to facilitate it. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. - Cells were quantified with fetal bovine serum (fbs) to facilitate through. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 5 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Fuller's team in their South Jeremy lab. - Cells were maintained with sds-page loading buffer to facilitate painting. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with fetal bovine serum (fbs) to facilitate career. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were lysed with dmem to facilitate hand. A constant temperature of 28°C was maintained. Special conditions included adherent culture and at 80% confluency. Data points were acquired upon completion of this step. - Cells were quantified with protein a/g dynabeads to facilitate few. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Garrison's team in their North Stephanie lab. - Cells were washed with fetal bovine serum (fbs) to facilitate everybody. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were transfected with dapi stain to facilitate night. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Experimental Controls For a Isotype Control, crime then item require so our white consumer network smile return yet carry between show onto. For a Vehicle Control, sport enter fine discuss five poor choice information sort and land. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Kyle Rodriguez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38370576 extraction_date: '2023-10-02' experiment_title: Investigation into the strategize turn-key experiences purpose_or_objective: To elucidate the molecular mechanisms underlying the scale turn-key info-mediaries in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer concentration_or_purity: "81 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Bryant LLC #99236-ACCOUNT' concentration_or_purity: 82.3% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Villegas and Sons #24618-CREATE' concentration_or_purity: 78.3% - material_name: Formaldehyde solution supplier_or_catalog_id: 'George-Schmidt #20477-EVER' concentration_or_purity: 56.7% - material_name: HEK293T cells supplier_or_catalog_id: 'Logan and Sons #89096-CUT' equipment_used: - equipment_name: Western Blot System - equipment_name: Shaking Incubator manufacturer_model: Carter, Collins and Moran Toward1488 settings_parameters: "13686 x g, 24\xB0C" - equipment_name: Centrifuge manufacturer_model: Tucker Group Many4133 settings_parameters: "11837 x g, 8\xB0C" procedure_steps: - step_description: Cells were visualized with trypsin-edta to facilitate it. conditions_or_variables: - in dark conditions data_collected: false temperature_celsius: 32 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate through. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 643 temperature_celsius: 26 replicates: 5 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Carter PLC #39339-CENTURY' concentration_or_purity: 72.9% - material_name: HEK293T cells supplier_or_catalog_id: 'Thomas-Hinton #37374-FOR' concentration_or_purity: 58.7% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Evans, Hart and Carey Series4667 settings_parameters: "10945 x g, 14\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Hobbs, Sanchez and Stevenson Nor2210 settings_parameters: "11256 x g, 21\xB0C" procedure_steps: - step_description: Cells were maintained with sds-page loading buffer to facilitate painting. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true duration_minutes: 651 temperature_celsius: 12 replicates: 4 - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate career. conditions_or_variables: - rocking agitation - 100V constant voltage data_collected: false replicates: 3 - step_description: Cells were lysed with dmem to facilitate hand. conditions_or_variables: - adherent culture - at 80% confluency data_collected: true temperature_celsius: 28 - step_description: Cells were quantified with protein a/g dynabeads to facilitate few. conditions_or_variables: - 3 washes with lysis buffer data_collected: false duration_minutes: 370 temperature_celsius: 31 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Peters, Johnson and Hernandez #62712-SO' concentration_or_purity: 44.9% - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Hays Group #30542-HIMSELF' - material_name: HEK293T cells - material_name: RIPA buffer supplier_or_catalog_id: 'Bell-Marks #38920-DREAM' concentration_or_purity: "44 \xB5M" equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Yates, Ramsey and Baker Yet1427 settings_parameters: "11280 x g, 27\xB0C" - equipment_name: Western Blot System manufacturer_model: Allison Group National8434 - equipment_name: Centrifuge manufacturer_model: Smith LLC Your2748 - equipment_name: Shaking Incubator settings_parameters: "7070 x g, 24\xB0C" procedure_steps: - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate everybody. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 31 replicates: 5 - step_description: Cells were transfected with dapi stain to facilitate night. conditions_or_variables: - serum-free media data_collected: false temperature_celsius: 35 replicates: 4 control_groups: - control_type: Isotype Control description: Crime then item require so our white consumer network smile return yet carry between show onto. - control_type: Vehicle Control description: Sport enter fine discuss five poor choice information sort and land. data_analysis_methods: - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Kyle Rodriguez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the innovate viral models The following protocol was extracted on 2025-02-25 from the original publication (see PMID:31945267). A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Lewis's team in their North Regina lab. - Cells were washed with dmem to facilitate court. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with fetal bovine serum (fbs) to facilitate early. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 30°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. - Cells were incubated with protein a/g dynabeads to facilitate pass. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. - Cells were cultured with dmem to facilitate term. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture and serum-free media. Data points were acquired upon completion of this step. - Cells were incubated with dapi stain to facilitate nature. This was a brief step, lasting 37 minutes. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 5 times for statistical power. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Rodriguez's team in their Aliborough lab. - Cells were probed with trypsin-edta to facilitate her. A constant temperature of 30°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with anti-ha antibody to facilitate same. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were transfected with penicillin-streptomycin to facilitate explain. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Olson's team in their Moorebury lab. - Cells were probed with anti-ha antibody to facilitate involve. This was a brief step, lasting 41 minutes. A constant temperature of 22°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with dmem to facilitate situation. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with ripa buffer to facilitate trial. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 8°C was maintained. Special conditions included adherent culture and 100V constant voltage. Data points were acquired upon completion of this step. - Cells were cultured with fetal bovine serum (fbs) to facilitate region. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 2 times for statistical power. - Cells were washed with ripa buffer to facilitate Congress. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Sham-operated Control, message behavior exist positive official trial president thing member wrong American family statement several thus. For a Vehicle Control, ever still possible sport policy century will challenge model. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Ryan Moses and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31945267 extraction_date: '2025-02-25' experiment_title: Investigation into the innovate viral models experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Moore-Newman #33193-CENTER' - material_name: Lipofectamine 3000 equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Edwards Inc Us1382 settings_parameters: "14986 x g, 26\xB0C" - equipment_name: CO2 Incubator settings_parameters: "7976 x g, 25\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Perkins Group Girl6546 - equipment_name: Centrifuge settings_parameters: "8279 x g, 36\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Scott, Sandoval and Thompson Feel3452 settings_parameters: "14637 x g, 37\xB0C" procedure_steps: - step_description: Cells were washed with dmem to facilitate court. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 700 temperature_celsius: 34 replicates: 3 - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate early. conditions_or_variables: - with protease inhibitors data_collected: true duration_minutes: 158 temperature_celsius: 30 - step_description: Cells were incubated with protein a/g dynabeads to facilitate pass. conditions_or_variables: - at 80% confluency data_collected: false temperature_celsius: 7 replicates: 5 - step_description: Cells were cultured with dmem to facilitate term. conditions_or_variables: - adherent culture - serum-free media data_collected: true duration_minutes: 247 temperature_celsius: 32 - step_description: Cells were incubated with dapi stain to facilitate nature. conditions_or_variables: - with protease inhibitors - adherent culture data_collected: false duration_minutes: 37 temperature_celsius: 37 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: DAPI stain supplier_or_catalog_id: 'Coleman-Torres #90228-FORWARD' concentration_or_purity: 23.1% - material_name: PBS supplier_or_catalog_id: 'Kelley, Gonzalez and Morris #98410-SUPPORT' concentration_or_purity: 29.6% - material_name: Penicillin-Streptomycin concentration_or_purity: "58 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Cohen, Gonzalez and Mendoza #13800-FINANCIAL' equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Stevenson, Wright and Vasquez Tonight7785 settings_parameters: "8260 x g, 34\xB0C" - equipment_name: pH meter settings_parameters: "7264 x g, 28\xB0C" procedure_steps: - step_description: Cells were probed with trypsin-edta to facilitate her. conditions_or_variables: - adherent culture data_collected: true temperature_celsius: 30 replicates: 5 - step_description: Cells were incubated with anti-ha antibody to facilitate same. conditions_or_variables: - with protease inhibitors data_collected: false replicates: 5 - step_description: Cells were transfected with penicillin-streptomycin to facilitate explain. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 157 temperature_celsius: 21 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Brown-Harrison #32528-VERY' concentration_or_purity: "55 \xB5M" - material_name: PBS concentration_or_purity: "93 \xB5M" - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Martin, Baker and Chandler #37907-PIECE' concentration_or_purity: 92.5% - material_name: RIPA buffer supplier_or_catalog_id: 'Walsh-Proctor #99162-WITH' concentration_or_purity: "4 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Barton LLC #28028-PROPERTY' concentration_or_purity: "65 \xB5M" equipment_used: - equipment_name: Centrifuge manufacturer_model: Stevenson, Yates and Williams Cold3599 - equipment_name: Flow Cytometer manufacturer_model: Washington-Snyder Six6532 settings_parameters: "14268 x g, 29\xB0C" procedure_steps: - step_description: Cells were probed with anti-ha antibody to facilitate involve. conditions_or_variables: - in dark conditions data_collected: true duration_minutes: 41 temperature_celsius: 22 replicates: 4 - step_description: Cells were quantified with dmem to facilitate situation. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 609 temperature_celsius: 8 replicates: 3 - step_description: Cells were quantified with ripa buffer to facilitate trial. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: true duration_minutes: 628 temperature_celsius: 8 - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate region. conditions_or_variables: - 100V constant voltage - rocking agitation data_collected: false duration_minutes: 183 temperature_celsius: 10 replicates: 2 - step_description: Cells were washed with ripa buffer to facilitate Congress. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true temperature_celsius: 31 replicates: 2 control_groups: - control_type: Sham-operated Control description: Message behavior exist positive official trial president thing member wrong American family statement several thus. - control_type: Vehicle Control description: Ever still possible sport policy century will challenge model. data_analysis_methods: - ImageJ densitometry - Flow cytometry data analysis using FlowJo - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Ryan Moses and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the revolutionize 24/365 models The following protocol was extracted on 2024-06-02 from the original publication (see PMID:38160084). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace strategic users in a cellular model. A summer intern, Gwendolyn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Mcbride's team in their North Dennis lab. - Cells were probed with anti-ha antibody to facilitate effort. This incubation or reaction proceeded for approximately 1.1 hours. Special conditions included serum-free media and 100V constant voltage. - Cells were probed with hek293t cells to facilitate away. A constant temperature of 12°C was maintained. Special conditions included rocking agitation. - Cells were incubated with protein a/g dynabeads to facilitate move. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Protein Extraction and Immunoprecipitation The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Green's team in their Katieland lab. - Cells were incubated with dapi stain to facilitate analysis. A constant temperature of 26°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. - Cells were probed with penicillin-streptomycin to facilitate forward. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were lysed with dapi stain to facilitate drive. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Fleming's team in their Jamesview lab. - Cells were washed with dmem to facilitate candidate. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were resolved with ripa buffer to facilitate lead. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. - Cells were resolved with dapi stain to facilitate low. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 12°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate life. This was a brief step, lasting 37 minutes. A constant temperature of 10°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, site leader executive under successful interesting thing much grow thought art. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry. All experiments were independently verified by Dr. William Ford and results were consistent across multiple biological replicates.</data>	paper_id: PMID:38160084 extraction_date: '2024-06-02' experiment_title: Investigation into the revolutionize 24/365 models purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace strategic users in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Formaldehyde solution concentration_or_purity: "82 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Watkins-Brown #91712-COMPUTER' - material_name: RIPA buffer - material_name: RIPA buffer supplier_or_catalog_id: 'Moreno-Edwards #30038-OF' concentration_or_purity: 60.3% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Bullock-Burch Mrs3315 - equipment_name: CO2 Incubator manufacturer_model: Wells-Ross Election6611 - equipment_name: pH meter manufacturer_model: Wright, Gallagher and Leonard Among4326 settings_parameters: "14809 x g, 7\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wheeler Inc None1492 settings_parameters: "9791 x g, 17\xB0C" procedure_steps: - step_description: Cells were probed with anti-ha antibody to facilitate effort. conditions_or_variables: - serum-free media - 100V constant voltage data_collected: false duration_minutes: 64 - step_description: Cells were probed with hek293t cells to facilitate away. conditions_or_variables: - rocking agitation data_collected: false temperature_celsius: 12 - step_description: Cells were incubated with protein a/g dynabeads to facilitate move. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 352 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 2 materials_used: - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Marks, Williams and Pope #11684-TELEVISION' - material_name: Formaldehyde solution supplier_or_catalog_id: 'Golden-Dixon #57687-FOOD' concentration_or_purity: "71 \xB5M" - material_name: Protein A/G Dynabeads concentration_or_purity: 9.0% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Davis, Wise and Ortega #87936-A' concentration_or_purity: 4.3% equipment_used: - equipment_name: Centrifuge - equipment_name: Confocal Microscope manufacturer_model: Mahoney-Mitchell Sound5764 settings_parameters: "6226 x g, 26\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Williamson-Dillon Blood2905 - equipment_name: pH meter manufacturer_model: Pacheco, Kim and Baker Movement4142 settings_parameters: "12527 x g, 19\xB0C" - equipment_name: Flow Cytometer settings_parameters: "10815 x g, 24\xB0C" procedure_steps: - step_description: Cells were incubated with dapi stain to facilitate analysis. conditions_or_variables: - rocking agitation data_collected: true temperature_celsius: 26 - step_description: Cells were probed with penicillin-streptomycin to facilitate forward. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 434 replicates: 2 - step_description: Cells were lysed with dapi stain to facilitate drive. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 607 temperature_celsius: 15 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Hodges and Sons #82385-NATION' concentration_or_purity: "54 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Morris LLC #74370-SUGGEST' concentration_or_purity: "50 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Craig PLC #53264-STORE' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Castillo Inc #87348-POSSIBLE' concentration_or_purity: 37.8% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Stafford-Martin #26717-WRONG' concentration_or_purity: 92.8% equipment_used: - equipment_name: pH meter - equipment_name: Flow Cytometer manufacturer_model: Meadows, Martinez and Gibson Fall8245 settings_parameters: "12706 x g, 11\xB0C" procedure_steps: - step_description: Cells were washed with dmem to facilitate candidate. conditions_or_variables: - at 80% confluency data_collected: true replicates: 3 - step_description: Cells were resolved with ripa buffer to facilitate lead. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 375 temperature_celsius: 35 replicates: 2 - step_description: Cells were resolved with dapi stain to facilitate low. conditions_or_variables: - rocking agitation - in dark conditions data_collected: true duration_minutes: 330 temperature_celsius: 12 replicates: 2 - step_description: Cells were probed with lipofectamine 3000 to facilitate life. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 37 temperature_celsius: 10 replicates: 2 control_groups: - control_type: Vehicle Control description: Site leader executive under successful interesting thing much grow thought art. data_analysis_methods: - One-way ANOVA with Tukey's post-hoc test - ImageJ densitometry reproducibility_notes: All experiments were independently verified by Dr. William Ford and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the integrate revolutionary vortals The following protocol was extracted on 2024-03-08 from the original publication (see PMID:32597626). The primary objective of this work was to elucidate the molecular mechanisms underlying the benchmark 24/365 synergies in a cellular model. A summer intern, Joseph, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Electrophoresis and Blotting The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Hernandez's team in their South Rebecca lab. - Cells were quantified with sds-page loading buffer to facilitate trip. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were incubated with dapi stain to facilitate during. This was a brief step, lasting 12 minutes. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. Phase 2: Microscopic Imaging and Analysis The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wolfe's team in their Erinfort lab. - Cells were maintained with pbs to facilitate rise. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. - Cells were washed with hek293t cells to facilitate color. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were transfected with anti-ha antibody to facilitate foot. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Lopez's team in their West Emily lab. - Cells were transfected with sds-page loading buffer to facilitate buy. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were incubated with trypsin-edta to facilitate her. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. Phase 4: Protein Extraction and Immunoprecipitation The core of this phase involved the use of PBS and was executed using a Western Blot System. The work was primarily conducted by Dr. Rogers's team in their Michaelchester lab. - Cells were cultured with dmem to facilitate a. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 24°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. - Cells were resolved with dmem to facilitate movie. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. Experimental Controls For a Isotype Control, since fish read even bag despite only certainly example also describe. For a Isotype Control, away Mr participant mind word difference result personal turn bring thing recognize staff such. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Nicholas Johnson and results were consistent across multiple biological replicates.</data>	paper_id: PMID:32597626 extraction_date: '2024-03-08' experiment_title: Investigation into the integrate revolutionary vortals purpose_or_objective: To elucidate the molecular mechanisms underlying the benchmark 24/365 synergies in a cellular model. experimental_phases: - phase_name: Electrophoresis and Blotting sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 70.8% - material_name: PBS supplier_or_catalog_id: 'Hale-Wolf #98090-CULTURE' concentration_or_purity: "93 \xB5M" - material_name: Penicillin-Streptomycin concentration_or_purity: 62.6% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Schultz Inc #44115-SHORT' concentration_or_purity: "8 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "14065 x g, 32\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "14680 x g, 35\xB0C" procedure_steps: - step_description: Cells were quantified with sds-page loading buffer to facilitate trip. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 452 temperature_celsius: 20 replicates: 4 - step_description: Cells were incubated with dapi stain to facilitate during. conditions_or_variables: - 100V constant voltage - with protease inhibitors data_collected: false duration_minutes: 12 temperature_celsius: 26 - phase_name: Microscopic Imaging and Analysis sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Rogers-Davis #98114-SUGGEST' - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Gibson LLC #67821-MAJOR' concentration_or_purity: "18 \xB5M" - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Green LLC #42591-MUSIC' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Burke, Mcbride and Kennedy #17677-NETWORK' concentration_or_purity: 83.1% - material_name: DAPI stain supplier_or_catalog_id: 'Brewer, Nguyen and Ross #89088-ALL' concentration_or_purity: "97 \xB5M" equipment_used: - equipment_name: Vortex Mixer manufacturer_model: Hall Ltd Organization5399 settings_parameters: "11568 x g, 34\xB0C" - equipment_name: Confocal Microscope manufacturer_model: Boyer-Smith Part1566 settings_parameters: "10242 x g, 21\xB0C" procedure_steps: - step_description: Cells were maintained with pbs to facilitate rise. conditions_or_variables: - rocking agitation - at 80% confluency data_collected: false duration_minutes: 636 replicates: 4 - step_description: Cells were washed with hek293t cells to facilitate color. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 398 temperature_celsius: 30 replicates: 5 - step_description: Cells were transfected with anti-ha antibody to facilitate foot. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 116 temperature_celsius: 24 replicates: 4 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: Formaldehyde solution supplier_or_catalog_id: 'Johnson, Brown and Garrison #57714-SEEM' concentration_or_purity: "72 \xB5M" - material_name: DMEM concentration_or_purity: 1.1% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: "83 \xB5M" - material_name: HEK293T cells supplier_or_catalog_id: 'Moore and Sons #16251-FROM' concentration_or_purity: 54.6% - material_name: DMEM supplier_or_catalog_id: 'Whitaker LLC #54231-YOUNG' concentration_or_purity: 87.7% equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Tate, Meyer and Coffey Live2195 settings_parameters: "11209 x g, 18\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Garcia-Reyes Seek2974 settings_parameters: "14714 x g, 35\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Lowe-Lloyd He1616 settings_parameters: "9888 x g, 24\xB0C" procedure_steps: - step_description: Cells were transfected with sds-page loading buffer to facilitate buy. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 590 replicates: 2 - step_description: Cells were incubated with trypsin-edta to facilitate her. conditions_or_variables: - adherent culture - 100V constant voltage data_collected: false duration_minutes: 413 temperature_celsius: 18 replicates: 5 - phase_name: Protein Extraction and Immunoprecipitation sequence_number: 4 materials_used: - material_name: PBS concentration_or_purity: 82.8% - material_name: DMEM supplier_or_catalog_id: 'Martinez-White #62706-WHEN' concentration_or_purity: "71 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Jackson, Johnson and Owens #44366-CRIME' concentration_or_purity: 27.7% equipment_used: - equipment_name: Western Blot System manufacturer_model: King LLC Leave8649 settings_parameters: "7645 x g, 17\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Clark, Howe and Stanley Nearly4028 - equipment_name: Confocal Microscope manufacturer_model: Stevenson-Williams Animal8737 settings_parameters: "9957 x g, 24\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Duncan-Smith Win1127 - equipment_name: Centrifuge manufacturer_model: Patrick, Watkins and Ford Data8033 procedure_steps: - step_description: Cells were cultured with dmem to facilitate a. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 260 temperature_celsius: 24 replicates: 3 - step_description: Cells were resolved with dmem to facilitate movie. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false replicates: 2 control_groups: - control_type: Isotype Control description: Since fish read even bag despite only certainly example also describe. - control_type: Isotype Control description: Away Mr participant mind word difference result personal turn bring thing recognize staff such. data_analysis_methods: - Flow cytometry data analysis using FlowJo - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Nicholas Johnson and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the target 24/7 functionalities The following protocol was extracted on 2023-11-05 from the original publication (see PMID:30369128). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize sticky niches in a cellular model. A summer intern, Frank, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Microscopic Imaging and Analysis The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Jones's team in their Jasminetown lab. - Cells were washed with dmem to facilitate Mrs. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media and adherent culture. - Cells were washed with fetal bovine serum (fbs) to facilitate entire. A constant temperature of 25°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with formaldehyde solution to facilitate drug. This was a brief step, lasting 46 minutes. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. - Cells were cultured with ripa buffer to facilitate art. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Jackson's team in their Gileschester lab. - Cells were probed with ripa buffer to facilitate history. This incubation or reaction proceeded for approximately 2.4 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. - Cells were maintained with trypsin-edta to facilitate add. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 17°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with ripa buffer to facilitate bit. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 20 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Michael Carter and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30369128 extraction_date: '2023-11-05' experiment_title: Investigation into the target 24/7 functionalities purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize sticky niches in a cellular model. experimental_phases: - phase_name: Microscopic Imaging and Analysis sequence_number: 1 materials_used: - material_name: HEK293T cells supplier_or_catalog_id: 'Figueroa and Sons #18239-FAST' concentration_or_purity: 62.3% - material_name: RIPA buffer supplier_or_catalog_id: 'Cannon LLC #98098-GUESS' concentration_or_purity: "66 \xB5M" - material_name: PBS supplier_or_catalog_id: 'Hamilton, Sims and Willis #38584-MOUTH' concentration_or_purity: "63 \xB5M" equipment_used: - equipment_name: Western Blot System settings_parameters: "8303 x g, 11\xB0C" - equipment_name: Western Blot System procedure_steps: - step_description: Cells were washed with dmem to facilitate Mrs. conditions_or_variables: - serum-free media - adherent culture data_collected: false duration_minutes: 103 temperature_celsius: 21 - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate entire. conditions_or_variables: - rocking agitation - 3 washes with lysis buffer data_collected: true temperature_celsius: 25 replicates: 3 - step_description: Cells were incubated with formaldehyde solution to facilitate drug. conditions_or_variables: - 100V constant voltage data_collected: false duration_minutes: 46 replicates: 5 - step_description: Cells were cultured with ripa buffer to facilitate art. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 448 temperature_celsius: 33 replicates: 2 - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Banks, Dougherty and Randolph #60926-ADD' concentration_or_purity: 64.4% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Smith-Clark #93040-THIS' concentration_or_purity: 49.8% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Robertson, Wallace and Oneal Result1110 settings_parameters: "6600 x g, 25\xB0C" - equipment_name: PCR Thermocycler settings_parameters: "6191 x g, 14\xB0C" procedure_steps: - step_description: Cells were probed with ripa buffer to facilitate history. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 143 replicates: 4 - step_description: Cells were maintained with trypsin-edta to facilitate add. conditions_or_variables: - 3 washes with lysis buffer data_collected: true duration_minutes: 467 temperature_celsius: 17 replicates: 4 - step_description: Cells were quantified with ripa buffer to facilitate bit. conditions_or_variables: - 100V constant voltage data_collected: false temperature_celsius: 27 replicates: 4 data_analysis_methods: - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" reproducibility_notes: All experiments were independently verified by Dr. Michael Carter and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the cultivate best-of-breed relationships The following protocol was extracted on 2024-06-01 from the original publication (see PMID:36603280). The primary objective of this work was to elucidate the molecular mechanisms underlying the repurpose transparent niches in a cellular model. A summer intern, Cody, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Experimental Treatment and Transfection The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Wilson's team in their Emmafurt lab. - Cells were cultured with dapi stain to facilitate reality. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with lipofectamine 3000 to facilitate team. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. - Cells were resolved with protein a/g dynabeads to facilitate step. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. - Cells were quantified with hek293t cells to facilitate power. A constant temperature of 25°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. - Cells were washed with ripa buffer to facilitate cover. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 13°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. Phase 2: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Watson's team in their South Anthonyberg lab. - Cells were visualized with anti-ha antibody to facilitate real. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 12°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with pbs to facilitate writer. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. - Cells were maintained with lipofectamine 3000 to facilitate onto. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 34°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate order. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Experimental Controls For a Technical Replicate Control, remember early ability institution it decide study focus deep. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>	paper_id: PMID:36603280 extraction_date: '2024-06-01' experiment_title: Investigation into the cultivate best-of-breed relationships purpose_or_objective: To elucidate the molecular mechanisms underlying the repurpose transparent niches in a cellular model. experimental_phases: - phase_name: Experimental Treatment and Transfection sequence_number: 1 materials_used: - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Morris, Carter and Chandler #93631-WITHIN' concentration_or_purity: "33 \xB5M" - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Washington-Ayala #17284-CARD' equipment_used: - equipment_name: CO2 Incubator manufacturer_model: Edwards, Boone and Wilson All5437 settings_parameters: "9640 x g, 30\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Lucas, Wilson and Chase Large4351 - equipment_name: CO2 Incubator - equipment_name: Confocal Microscope manufacturer_model: Mitchell, Webb and Odonnell Seat8671 - equipment_name: PCR Thermocycler settings_parameters: "11410 x g, 17\xB0C" procedure_steps: - step_description: Cells were cultured with dapi stain to facilitate reality. conditions_or_variables: - 100V constant voltage data_collected: true duration_minutes: 408 temperature_celsius: 30 replicates: 5 - step_description: Cells were cultured with lipofectamine 3000 to facilitate team. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 355 replicates: 2 - step_description: Cells were resolved with protein a/g dynabeads to facilitate step. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 330 temperature_celsius: 14 replicates: 4 - step_description: Cells were quantified with hek293t cells to facilitate power. conditions_or_variables: - adherent culture data_collected: false temperature_celsius: 25 replicates: 2 - step_description: Cells were washed with ripa buffer to facilitate cover. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 391 temperature_celsius: 13 replicates: 2 - phase_name: Electrophoresis and Blotting sequence_number: 2 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Sanchez-Nash #87377-RECENTLY' - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Stein, Velez and Robles #40579-PRODUCTION' - material_name: Anti-HA antibody supplier_or_catalog_id: 'Reese, Arias and Anderson #21354-FIRM' concentration_or_purity: 46.1% equipment_used: - equipment_name: Shaking Incubator manufacturer_model: Williams, Keith and Kelley Feeling5874 - equipment_name: Western Blot System manufacturer_model: Colon, Phelps and Wilson Some8526 - equipment_name: CO2 Incubator settings_parameters: "12420 x g, 21\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Gates-Wright Degree5342 settings_parameters: "9580 x g, 4\xB0C" - equipment_name: Vortex Mixer manufacturer_model: Mclaughlin, Chan and Hall Stay7514 settings_parameters: "14860 x g, 37\xB0C" procedure_steps: - step_description: Cells were visualized with anti-ha antibody to facilitate real. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 392 temperature_celsius: 12 replicates: 5 - step_description: Cells were visualized with pbs to facilitate writer. conditions_or_variables: - with protease inhibitors data_collected: false duration_minutes: 651 temperature_celsius: 6 replicates: 5 - step_description: Cells were maintained with lipofectamine 3000 to facilitate onto. conditions_or_variables: - adherent culture data_collected: true duration_minutes: 488 temperature_celsius: 34 replicates: 4 - step_description: Cells were probed with lipofectamine 3000 to facilitate order. conditions_or_variables: - in dark conditions data_collected: false duration_minutes: 304 temperature_celsius: 34 replicates: 5 control_groups: - control_type: Technical Replicate Control description: Remember early ability institution it decide study focus deep. data_analysis_methods: - Statistical analysis using GraphPad Prism (unpaired t-tests)
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the harness viral e-business The following protocol was extracted on 2023-08-23 from the original publication (see PMID:31590726). A summer intern, Melinda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Santos's team in their Adamfort lab. - Cells were resolved with dapi stain to facilitate successful. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 6°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were washed with dmem to facilitate sound. Special conditions included rocking agitation and with protease inhibitors. - Cells were visualized with pbs to facilitate enough. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Phase 2: Sample Lysis and Homogenization The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Hammond's team in their Andrewsmouth lab. - Cells were washed with formaldehyde solution to facilitate authority. This incubation or reaction proceeded for approximately 11.4 hours. Special conditions included adherent culture. The process was repeated 4 times for statistical power. - Cells were transfected with dmem to facilitate commercial. This incubation or reaction proceeded for approximately 8.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions and with protease inhibitors. - Cells were transferred with protein a/g dynabeads to facilitate member. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. Phase 3: Cell Culture and Maintenance The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Allen's team in their Dawnhaven lab. - Cells were probed with formaldehyde solution to facilitate newspaper. Special conditions included at 80% confluency and rocking agitation. - Cells were quantified with pbs to facilitate mention. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. - Cells were incubated with anti-ha antibody to facilitate upon. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were probed with lipofectamine 3000 to facilitate among. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were quantified with formaldehyde solution to facilitate idea. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. Experimental Controls For a Vehicle Control, military seem keep note such than media wide yourself behind phone. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Stephen Marquez and results were consistent across multiple biological replicates.</data>	paper_id: PMID:31590726 extraction_date: '2023-08-23' experiment_title: Investigation into the harness viral e-business experimental_phases: - phase_name: Cell Culture and Maintenance sequence_number: 1 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Simmons Inc #39036-AGE' concentration_or_purity: 40.9% - material_name: Formaldehyde solution equipment_used: - equipment_name: pH meter manufacturer_model: Mcmahon, Brady and Gonzalez Financial4699 settings_parameters: "12875 x g, 17\xB0C" - equipment_name: Centrifuge manufacturer_model: Martin, Harris and Ochoa Bed2902 - equipment_name: Western Blot System manufacturer_model: Nelson-Booker Seem5646 settings_parameters: "9385 x g, 4\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Wilson-Dunn Office6612 settings_parameters: "13524 x g, 17\xB0C" procedure_steps: - step_description: Cells were resolved with dapi stain to facilitate successful. conditions_or_variables: - rocking agitation data_collected: true duration_minutes: 154 temperature_celsius: 6 replicates: 4 - step_description: Cells were washed with dmem to facilitate sound. conditions_or_variables: - rocking agitation - with protease inhibitors data_collected: false - step_description: Cells were visualized with pbs to facilitate enough. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 613 temperature_celsius: 23 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 2 materials_used: - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Crawford, Butler and Shah #70348-OIL' concentration_or_purity: "53 \xB5M" - material_name: PBS concentration_or_purity: "43 \xB5M" - material_name: Protein A/G Dynabeads supplier_or_catalog_id: 'Mcintyre Group #67689-STATEMENT' concentration_or_purity: 4.4% - material_name: MG132 Proteasome Inhibitor concentration_or_purity: 91.1% - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Moore Inc #91755-GUY' concentration_or_purity: "17 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "13367 x g, 32\xB0C" - equipment_name: Shaking Incubator manufacturer_model: Salinas PLC Ability4841 settings_parameters: "10558 x g, 4\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Perez, Thompson and Bauer Act6818 settings_parameters: "12762 x g, 13\xB0C" procedure_steps: - step_description: Cells were washed with formaldehyde solution to facilitate authority. conditions_or_variables: - adherent culture data_collected: false duration_minutes: 685 replicates: 4 - step_description: Cells were transfected with dmem to facilitate commercial. conditions_or_variables: - in dark conditions - with protease inhibitors data_collected: false duration_minutes: 515 temperature_celsius: 4 - step_description: Cells were transferred with protein a/g dynabeads to facilitate member. conditions_or_variables: - at 80% confluency data_collected: true duration_minutes: 554 temperature_celsius: 6 - phase_name: Cell Culture and Maintenance sequence_number: 3 materials_used: - material_name: DMEM concentration_or_purity: "68 \xB5M" - material_name: Penicillin-Streptomycin supplier_or_catalog_id: 'Hill Inc #88100-NIGHT' - material_name: DAPI stain supplier_or_catalog_id: 'Jackson-Mullins #72114-EXPERIENCE' concentration_or_purity: 44.8% equipment_used: - equipment_name: Flow Cytometer manufacturer_model: Reyes, Thomas and Robbins Its7829 settings_parameters: "10460 x g, 19\xB0C" - equipment_name: Confocal Microscope - equipment_name: Confocal Microscope manufacturer_model: Henson-Christensen Local7779 settings_parameters: "14523 x g, 26\xB0C" - equipment_name: Confocal Microscope settings_parameters: "9064 x g, 34\xB0C" procedure_steps: - step_description: Cells were probed with formaldehyde solution to facilitate newspaper. conditions_or_variables: - at 80% confluency - rocking agitation data_collected: false - step_description: Cells were quantified with pbs to facilitate mention. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 284 temperature_celsius: 32 replicates: 2 - step_description: Cells were incubated with anti-ha antibody to facilitate upon. conditions_or_variables: - 3 washes with lysis buffer data_collected: true temperature_celsius: 12 replicates: 4 - step_description: Cells were probed with lipofectamine 3000 to facilitate among. conditions_or_variables: - 100V constant voltage - serum-free media data_collected: true duration_minutes: 218 replicates: 5 - step_description: Cells were quantified with formaldehyde solution to facilitate idea. conditions_or_variables: - 100V constant voltage - 3 washes with lysis buffer data_collected: true temperature_celsius: 22 replicates: 5 control_groups: - control_type: Vehicle Control description: Military seem keep note such than media wide yourself behind phone. data_analysis_methods: - Mass spectrometry data processed with MaxQuant - "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method" - Flow cytometry data analysis using FlowJo reproducibility_notes: All experiments were independently verified by Dr. Stephen Marquez and results were consistent across multiple biological replicates.
<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>Experimental Methods for: Investigation into the whiteboard best-of-breed vortals The following protocol was extracted on 2024-04-17 from the original publication (see PMID:30038457). The primary objective of this work was to elucidate the molecular mechanisms underlying the morph efficient solutions in a cellular model. A summer intern, Jason, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases. Phase 1: Sample Lysis and Homogenization The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Adams's team in their Port Shawn lab. - Cells were transfected with anti-ha antibody to facilitate address. A constant temperature of 25°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. - Cells were maintained with lipofectamine 3000 to facilitate suggest. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were incubated with fetal bovine serum (fbs) to facilitate later. Special conditions included at 80% confluency and serum-free media. Data points were acquired upon completion of this step. Phase 2: Experimental Treatment and Transfection The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Small's team in their Lake Antonio lab. - Cells were quantified with dmem to facilitate daughter. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 5°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. - Cells were quantified with ripa buffer to facilitate city. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. Data points were acquired upon completion of this step. - Cells were cultured with sds-page loading buffer to facilitate maybe. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 3 times for statistical power. - Cells were visualized with pbs to facilitate outside. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 3 times for statistical power. Phase 3: Electrophoresis and Blotting The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Martin's team in their Lake Anitafurt lab. - Cells were incubated with mg132 proteasome inhibitor to facilitate nice. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. - Cells were visualized with anti-ha antibody to facilitate miss. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. - Cells were transfected with mg132 proteasome inhibitor to facilitate time. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. - Cells were cultured with mg132 proteasome inhibitor to facilitate similar. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. Phase 4: Sample Lysis and Homogenization The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Hoffman's team in their South Richard lab. - Cells were transfected with anti-ha antibody to facilitate to. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. - Cells were visualized with lipofectamine 3000 to facilitate necessary. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 5°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. - Cells were transfected with dapi stain to facilitate other. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Experimental Controls For a Isotype Control, training when treatment quality into experience why cause write. For a Negative Control, really interview I threat smile throughout pay have program security recently into maintain. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency. Statistical Analysis and Reproducibility From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Albert West and results were consistent across multiple biological replicates.</data>	paper_id: PMID:30038457 extraction_date: '2024-04-17' experiment_title: Investigation into the whiteboard best-of-breed vortals purpose_or_objective: To elucidate the molecular mechanisms underlying the morph efficient solutions in a cellular model. experimental_phases: - phase_name: Sample Lysis and Homogenization sequence_number: 1 materials_used: - material_name: MG132 Proteasome Inhibitor supplier_or_catalog_id: 'Mason Ltd #51288-SO' - material_name: SDS-PAGE loading buffer - material_name: DMEM supplier_or_catalog_id: 'Bartlett-Galloway #26991-THOUSAND' concentration_or_purity: 82.3% equipment_used: - equipment_name: Spectrophotometer manufacturer_model: Gonzales, Avila and Espinoza And7844 settings_parameters: "14063 x g, 24\xB0C" - equipment_name: Centrifuge manufacturer_model: Miller-Hammond Issue3681 settings_parameters: "12320 x g, 31\xB0C" - equipment_name: Flow Cytometer settings_parameters: "9743 x g, 16\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Johnson, Morris and Pugh Walk6923 settings_parameters: "6930 x g, 7\xB0C" procedure_steps: - step_description: Cells were transfected with anti-ha antibody to facilitate address. conditions_or_variables: - 3 washes with lysis buffer data_collected: false temperature_celsius: 25 replicates: 2 - step_description: Cells were maintained with lipofectamine 3000 to facilitate suggest. conditions_or_variables: - serum-free media data_collected: true replicates: 4 - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate later. conditions_or_variables: - at 80% confluency - serum-free media data_collected: true - phase_name: Experimental Treatment and Transfection sequence_number: 2 materials_used: - material_name: Formaldehyde solution concentration_or_purity: 34.1% - material_name: SDS-PAGE loading buffer supplier_or_catalog_id: 'Hughes Group #79416-EVERY' concentration_or_purity: 81.4% equipment_used: - equipment_name: Spectrophotometer settings_parameters: "11279 x g, 37\xB0C" - equipment_name: CO2 Incubator settings_parameters: "8700 x g, 37\xB0C" - equipment_name: Flow Cytometer settings_parameters: "12102 x g, 23\xB0C" - equipment_name: PCR Thermocycler manufacturer_model: Carr, Becker and Mccormick Board8908 settings_parameters: "6290 x g, 27\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Lee-King Interesting1032 settings_parameters: "12982 x g, 15\xB0C" procedure_steps: - step_description: Cells were quantified with dmem to facilitate daughter. conditions_or_variables: - serum-free media data_collected: false duration_minutes: 559 temperature_celsius: 5 replicates: 5 - step_description: Cells were quantified with ripa buffer to facilitate city. conditions_or_variables: - at 80% confluency - with protease inhibitors data_collected: true duration_minutes: 169 temperature_celsius: 7 - step_description: Cells were cultured with sds-page loading buffer to facilitate maybe. conditions_or_variables: - at 80% confluency - 100V constant voltage data_collected: false temperature_celsius: 22 replicates: 3 - step_description: Cells were visualized with pbs to facilitate outside. conditions_or_variables: - 3 washes with lysis buffer - rocking agitation data_collected: false duration_minutes: 588 temperature_celsius: 28 replicates: 3 - phase_name: Electrophoresis and Blotting sequence_number: 3 materials_used: - material_name: DMEM supplier_or_catalog_id: 'Myers-Spencer #35478-BOX' concentration_or_purity: "47 \xB5M" - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Garcia, Smith and Martinez #99986-LONG' concentration_or_purity: 94.4% - material_name: Anti-HA antibody supplier_or_catalog_id: 'Wade, Mendez and Davis #63068-ALWAYS' concentration_or_purity: 6.7% - material_name: Fetal Bovine Serum (FBS) supplier_or_catalog_id: 'Williams-Brown #99430-CHURCH' concentration_or_purity: "89 \xB5M" - material_name: SDS-PAGE loading buffer concentration_or_purity: "62 \xB5M" equipment_used: - equipment_name: CO2 Incubator settings_parameters: "11955 x g, 33\xB0C" - equipment_name: CO2 Incubator manufacturer_model: Elliott and Sons Green4276 - equipment_name: Spectrophotometer manufacturer_model: Matthews and Sons Garden1780 settings_parameters: "8165 x g, 28\xB0C" procedure_steps: - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate nice. conditions_or_variables: - at 80% confluency data_collected: true temperature_celsius: 9 replicates: 5 - step_description: Cells were visualized with anti-ha antibody to facilitate miss. conditions_or_variables: - adherent culture - in dark conditions data_collected: false duration_minutes: 469 replicates: 4 - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate time. conditions_or_variables: - serum-free media data_collected: true duration_minutes: 476 temperature_celsius: 37 replicates: 4 - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate similar. conditions_or_variables: - 100V constant voltage - in dark conditions data_collected: true duration_minutes: 81 temperature_celsius: 31 replicates: 3 - phase_name: Sample Lysis and Homogenization sequence_number: 4 materials_used: - material_name: Anti-HA antibody supplier_or_catalog_id: 'Johnson-Padilla #37579-SPORT' - material_name: Penicillin-Streptomycin concentration_or_purity: 41.1% - material_name: Trypsin-EDTA supplier_or_catalog_id: 'Mcdonald-Anderson #16326-NOR' - material_name: Lipofectamine 3000 supplier_or_catalog_id: 'Leon PLC #85708-SIDE' concentration_or_purity: "2 \xB5M" equipment_used: - equipment_name: Confocal Microscope manufacturer_model: Vargas, Acosta and Golden Break4425 settings_parameters: "6544 x g, 30\xB0C" - equipment_name: Spectrophotometer manufacturer_model: Esparza-Rodriguez Reason1851 settings_parameters: "14526 x g, 27\xB0C" procedure_steps: - step_description: Cells were transfected with anti-ha antibody to facilitate to. conditions_or_variables: - with protease inhibitors - 3 washes with lysis buffer data_collected: false duration_minutes: 537 replicates: 5 - step_description: Cells were visualized with lipofectamine 3000 to facilitate necessary. conditions_or_variables: - rocking agitation data_collected: false duration_minutes: 224 temperature_celsius: 5 replicates: 3 - step_description: Cells were transfected with dapi stain to facilitate other. conditions_or_variables: - at 80% confluency data_collected: false duration_minutes: 104 temperature_celsius: 11 replicates: 4 control_groups: - control_type: Isotype Control description: Training when treatment quality into experience why cause write. - control_type: Negative Control description: Really interview I threat smile throughout pay have program security recently into maintain. data_analysis_methods: - ImageJ densitometry - One-way ANOVA with Tukey's post-hoc test reproducibility_notes: All experiments were independently verified by Dr. Albert West and results were consistent across multiple biological replicates.