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METHODS |
A library of Gluconobacter oxydans H24 genomic DNA was constructed with host strains Escherichia coli JM109s, which was integrated of sdh gene at the ptsG site on the chromosome of JM109. By detecting sorbose-dehydrogenase activity, clone of PQQ biosynthesis was isolated and subcloned. |
RESULTS |
A positive clone was isolated from Gluconobacter oxydans H24 genomic DNA library. Within the 5,400-base-pair DNA fragment five reading frames are presented, corresponding to five of the pqq genes (pqqABCDE). The nucleotide and amino acid sequence showed highly homology to pqq genes of other bacteria. |
CONCLUSION |
The pqqABCDE gene cluster was successfully isolated from Gluconobacter oxydans H24 by sorbose dehydrogenase activity.Sudden worsening of DRESS syndrome on tapering steroid dose with dramatic improvement on N-acetylcysteine and steroid dose escalation |
Drug Reaction with Eosinophilia and Systemic Symptoms (DRESS) is a serious and life-threatening drug reaction characterized by fever, skin rash, peripheral eosinophilia, presence of atypical lymphocytes in peripheral blood smear, generalized lymphadenopathy and involvement of other body organs. The only agreed-upon the... |
The aim of the study was to investigate whether an intervention by the patient's general practitioner (GP) changes the consumption of medicine in nursing homes. It was designed as an intervention study including 111 inhabitants in nursing homes in a municipality (Odder). All the general practitioners in this local auth... |
One of the major challenges in robotics consists in developing successful control strategies for robotic grasping devices. In this scenario, one of the most interesting approaches regards the exploitation of surface electromyography(sEMG). In this work, we propose a novel sEMG-based minimally supervised regression appr... |
www.philadelphiafed.org *the views expressed here are those of the author and do not necessarily represent the views of the Federal reserve bank of Philadelphia or the Federal reserve system. links to most of the papers presented can be found on the Philadelphia Fed’s website at: http://www.philadelphiafed.org/research... |
Six unknown compounds present in bulk gentamicin samples have been identified by ion-pairing reversed-phase liquid chromatography coupled with ion trap mass spectrometry. The structures of these unknown compounds were deduced by comparison of their fragmentation patterns with those of the available related substances a... |
Abstract To characterize the bovine chromosome 6q21 for bovine chondrodysplastic dwarfism (BCD), we developed 48 new microsatellite markers from yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) clones using a modified magnetic bead capture method. These new markers were used to construct a hi... |
A vast body of literature demonstrated that anthropogenic disturbances such as overgrazing and fire are key drivers of abrupt transition between vegetation types in ecosystems. In this study, we propose that the hydrological context (described in terms of rainfall, evapotranspiration and water yield) is a first‐order, ... |
PURPOSE |
To determine overall response (OR) and complete response (CR) rates, response duration, progression-free (PFS) and overall survival and safety with the tositumomab and iodine-131 tositumomab ((131)I tositumomab) therapeutic regimen in patients with indolent, follicular large-cell, or transformed B-cell lymphoma, progre... |
PATIENTS AND METHODS |
From July 1998 to November 1999, 40 patients (24 rituximab nonresponders: 11 with response < 6 months, and five with response > or = 6 months) received a therapeutic dose (0.65 to 0.75 Gy per platelet count) of (131)I tositumomab based on total-body dosimetry in this prospective phase II study. The median number of pri... |
RESULTS |
Confirmed OR (65%) and CR (38%) rates were not significantly associated with prior rituximab response. With a median follow-up of 3.3 years, the median PFS was 10.4 months, 24.5 months for responders, and not reached for CR patients. Among follicular grade 1 or 2 patients with tumors < or = 7 cm (n = 21), the OR and CR... |
CONCLUSION |
(131)I tositumomab is effective in CD20-positive lymphoma progressive after rituximab, with a 65% OR rate and median PFS of 24.5 months for responders. Patients with follicular grade 1 or 2 histology and tumors < or = 7 cm achieved very high OR and CR rates, with 48% PFS at 3 years.Predictive control of PEMFC based on ... |
The modelling and control of proton exchange membrane fuel cell (PEMFC) possesses great challenges due to PEMFC system's inherent nonlinearities, time-varying characteristics and tight operating constraints. In this paper, we propose a constrained model predictive control (MPC) strategy based on a combined empirical an... |
The effect of cryogenic processing and tempering on selected mechanical properties and fracture micromechanism of Vanadis 6 high alloy cold work tool steel was analysed. The samples were processed in cold nitrogen gas at -140 °C for 48 h, and tempered at temperatures of 170 – 530 °C. It was found that the hardness of s... |
This paper begins by locating Education for Sustainable Development (ESD) in the context of education more broadly and identifies some key concepts that form a backcloth to understanding it and some tensions and curriculum issues to which it gives rise. The paper examines some of the strengths and problems of different... |
Vertical concentration profiles of soluble reactive phosphorus (SRP) in the upper sedimentary zone of freshwater lakes are an important means for studying internal phosphorus (P) loading and to gain insight into early diagenetic processes. The interpretation of such pore water profiles generally neglects the occurrence... |
A hydraulic pump is disclosed having an adjusting member for varying yoke angle. The present adjusting member includes a control piston for displacing the yoke so as to define a desired yoke angle. A stop member is provided having threads and retained within the control piston. An adjusting screw for rotational motion ... |
This chapter examines the regulation of chemicals, with emphasis on commonalities and differences in regulatory approaches. It begins with a brief overview of key concepts that underlie chemicals regulation, explaining what chemicals regulation is, the hazards and risks associated with chemicals, policy principles, inf... |
Abstract Early failure of knee replacements is thought to be due to the combination of sterilization by gamma irradiation in air and the high cyclic stresses that they endure during use. Such failures are shown through delamination and permanent deformation of the ultra-high molecular weight polyethylene (UHMWPE) compo... |
This article reviews and organizes the recent literature on psychosocial problems and interventions in diabetes to see if it is possible to identify effective modes of treatment for numerous different psychosocial problems. An attempt was made to review extensively the references on psychosocial problems and to review ... |
Phase morphofunctional alterations determining the stages of development of the intestinal deficiency syndrome were detected in a composite investigation of the motor, evacuatory, secretory, digestive and absorbing functions of the small intestine in experimental animals in the immediate period after operations on the ... |
This special issue of the International Journal of Bilingualism represents an important contribution to the field of code-switching (CS) not only because it is a joint collaboration between scholars based at the University of Ghana and New York University, but also because it presents new CS data sets. The contribution... |
Objective: To assess the uterine hemodynamics in gestational trophoblastic tumors and to correlate them with response to chemotherapy. Methods: Using transvaginal color Doppler ultrasound, we measured the peak systolic velocity and the resistance index (RI) of the uterine arteries in 23 women with gestational trophobla... |
We examine the coverage dependence of the photoelectron spectra in a generic two-sublattice overlayer model constructed in the Hubbard formalism. Using numerical exact diagonalization techniques, photoemission and inverse photoemission spectra are calculated. The single-particle hopping integrals in the overlayer and b... |
Method for producing granular detergent compositions or powder form, which comprises preparing a base powder detergent by drying an aqueous slurry of detergent, wherein the slurry is added a copolymer obtained by radical copolymerization of ( a) from 50 to 99.5 mol% of a monoethylenically unsaturated and / or a salt th... |
Evidence has accumulated concerning the association of micro-organisms of the genus Chlamydia with Reiter's syndrome (RS). Isolations have been reported from the conjunctiva, genital tract, and synovial fluid or tissue, and antichlamydial complement-fixing (CF) antibodies have been demonstrated in the sera of patients ... |
Labor historians have always been better at explaining what should have been than what was. This problem plagues Paul Buhle's Taking Care of Business: Samuel Gompers, George Meany, Lane Kirkland and the Tragedy of American Labor. No one familiar with Dr. Buhle's work—his detailed treatments of socialist radicals in the... |
In this research paper, consists of compressing the images within each pixel before the storage processes, hence the size of the memory gets reduced. This can be done by the proposed method namely block based compression algorithm which uses the differential coding scheme. Here, differential values are captured and the... |
Mid-level health providers (MLP) are health workers trained at a higher education institution for at least a total of 2-3 years, and authorized and regulated to work autonomously to diagnose, manage and treat illness, disease and impairments, as well as engage in preventive and promotive care. Their role has been progr... |
This study shows the human cellular responses and the mechanism of low-dose ionizing radiation in CCD 18 Lu cells, which are derived from normal human lung fibroblasts. Cell proliferation and viability assay were measured for the cells following gamma-irradiation using trypan blue, BrdU incorporation, and Wst-1 assay. ... |
We fabricated a back-side illuminated (BSI) complementary metal oxide semiconductor (CMOS) image sensor in which a very-thin BSI photodiode array chip was stacked on a CMOS read-out circuit chip by compliant bumps. Cone-shaped bumps made of Au were prepared as the compliant bumps. The base diameter was 10–12 µm and the... |
Plant morphogenesis depends on the synchronized anisotropic expansion of individual cells in response to developmental and environmental cues. The magnitude of cell expansion depends on the biomechanical properties of the cell wall, which in turn depends on both its biosynthesis and extensibility. Although the control ... |
As people are living longer and enjoying better quality of life, there has been a corresponding increase in interest in recreational music-making by adults. This study examined the demographics and motivations of students enrolled in the Community Piano Experience hosted by the University of Nebraska-Lincoln School of ... |
This study examines the effect of Er2O3 addition (0 to 2.0 mol.%) on microstructure and electrical characteristics of ZnO-based varistor ceramics prepared by high energy ball milling and sintering at 1200 °C for 1 h. With increasing Er2O3 content, the densification process diminishes marginally, but the grain size redu... |
We discuss a generalization of Parikh's Theorem for context- free languages to classes of many-sorted relational structures which are both definable in Monadic Second Order Logic and which are of bounded patch-width. Patch-width is a generalization of both tree-width and clique-width. This gives a powerful unifying too... |
Massive multiple-input multiple-output (MIMO) is becoming a mature technology, and has been approved for standardization in the 5G space. Although there are many papers on the theoretical analysis of massive MIMO, the majority of relevant work assumes the simplified, yet overly idealistic, Kronecker-type model for spat... |
Radioactive short‐chain fatty acids preferentially label glutamine relative to glutamate in brain due to compartmentation of glutamine and glutamate. To determine whether this phenomenon occurs in a single cell culture model, we examined the effect of fatty acid chain length on the synthesis as well as pool size of sel... |
A new rotor for the ABAC system has been investigated that allows the main enterobacteria of urinary source to be identified (this is performed manually) and simultaneously the relative antibiogram (this is automatically carried out). The precision of this identification has been shown using control strains previously ... |
The education sector is subject to significant changes due to the increasingly active spread of digital technologies. Usually, the trends in the implementation of digital technologies in educational and research activities are set by commercial organizations – private universities, business schools, corporate universit... |
Membrane chromatography possesses numerous advantages such as operation at high flow rates, low back pressure, ease of handling and scale up, which make the membrane adsorber process a viable alternative to conventional packed column chromatography. A purification process for the isolation of human recombinant basic fi... |
The risk of traumatic injury and low Apgar score was studied in 473 infants with a birth weight of 4500 g or more at term (LFD) and 473 infants with normal weight (NFD, birth weight +/- 1 SD of mean for the respective gestational age). The LFD group comprised 3.2% of all infants delivered during a 5-year period. Trauma... |
Every month, numerous papers appear in the popular scientific journals, including Biology of Reproduction, describing the expression of candidate genes, or the global gene expression pattern, in mammalian embryos. Many of these studies are very interesting and cleverly designed, and all aim to give us better insight in... |
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