IdA string | IdB string | labels int64 | mechanism string | effect string | score float64 | sentence string | signor_id string |
|---|---|---|---|---|---|---|---|
Q14498 | P00519 | 0 | phosphorylation | up-regulates activity | 0.322 | In this paper, we report that RBM39 interacts with the nonreceptor tyrosine kinase c-Abl. Both the Src homology (SH) 2 and SH3 domains of c-Abl interact with RBM39. The major tyrosine phosphorylation sites on RBM39 that are phosphorylated by c-Abl are Y95 and Y99, as demonstrated by liquid chromatography coupled with t... | SIGNOR-262609 |
P18031 | P49760 | 0 | phosphorylation | up-regulates activity | 0.322 | The clk family kinases, clk1 and clk2, phosphorylate and activate the tyrosine phosphatase, ptp-1b.|Phosphorylation of PTP-1B at Ser(50) by CLK1 or CLK2 is responsible for its enzymatic activation. | SIGNOR-70603 |
P31749 | Q99683 | 1 | phosphorylation | down-regulates activity | 0.729 | Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1 akt decreased ask1 kinase activity stimulated by both oxidative stress and overexpression in 293 cells by phosphorylating a consensus akt site at serine 83 of ask1. | SIGNOR-252465 |
P49841 | P35869 | 1 | phosphorylation | up-regulates activity | 0.25 | A proposed model of GSK3β role on AHR function and degradation. AHR is phosphorylated by GSK3β in a p23-dependent manner in HeLa cells. This phosphorylation is required for optimal activation of the ligand-dependent AHR target gene transcription. After phosphorylation, AHR is K63-ubiquitinated and is targeted for the L... | SIGNOR-276664 |
P63000 | O14559 | 0 | gtpase-activating protein | down-regulates activity | 0.444 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260491 |
Q16236 | Q13131 | 0 | phosphorylation | up-regulates activity | 0.2 | MS-based analysis of immunoprecipitated Nrf2 revealed serine 374, 408 and 433 in human Nrf2 to be hyperphosphorylated as a function of activated AMPK. A direct phosphate-transfer by AMPK to those sites was indicated by in vitro kinase assays with recombinant proteins as well as interaction of AMPK and Nrf2 in cells, ev... | SIGNOR-277496 |
P14780 | P01137 | 1 | cleavage | up-regulates | 0.592 | We also demonstrate that mmp-9, as well as its relative, mmp-2, cleave latent transforming growth factor-_ (tgf-_), which constitutes a novel mechanism of tgf-_ activation. | SIGNOR-74461 |
Q5JU85 | P48058 | 1 | relocalization | up-regulates quantity | 0.2 | BRAG1 increases the synaptic recycling pool of AMPARs.these data suggest that the BRAG1 enhancement of AMPAR transmission is mediated by the increased expression of the recycling pool of synaptic GluA2/3 receptors. | SIGNOR-264915 |
O00311 | Q8TEA8 | 1 | phosphorylation | up-regulates activity | 0.321 | Here we show that DUE-B is de-phosphorylated in M phase and phosphorylated in G1/S phase. Phosphorylated DUE-B forms homodimers, whereas de-phosphorylated DUE-B interacts with the Mcm2–7 complex and aminoacyl-tRNA synthetases. We find that CKII can prime DUE-B for Cdc7 phosphorylation. Confirming the importance of DUE... | SIGNOR-273967 |
Q9Y261 | P80370 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.332 | Taken together, these data suggest that Foxa-2 is a direct transcriptional activator of the Pref-1 gene. | SIGNOR-254971 |
Q8NFJ5 | P00533 | 0 | phosphorylation | down-regulates activity | 0.387 | EGFR phosphorylates and inhibits lung tumor suppressor GPRC5A in lung cancer.|Together, these results indicate that endogenous EGFR can phosphorylate GPRC5A at four identified tyrosine residues (Y317, Y320, Y347 and Y350) in response to EGF stimulation in the lung cancer H1792 cells. | SIGNOR-278336 |
O14827 | P63000 | 1 | guanine nucleotide exchange factor | up-regulates activity | 0.498 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260574 |
Q99496 | Q9C0C7 | 1 | ubiquitination | down-regulates quantity by destabilization | 0.359 | RNF2 ubiquitinates AMBRA1 at lysine 45.|These data indicate that RNF2 directly accelerates the degradation of AMBRA1. | SIGNOR-278596 |
O15111 | Q9Y6Q9 | 1 | phosphorylation | up-regulates | 0.395 | Herein, we report the successful identification of six functional in vivo src-3 phosphorylation sites. | SIGNOR-196953 |
P68400 | Q04724 | 1 | phosphorylation | up-regulates | 0.32 | These results suggest that ck2 phosphorylation of serine 239 of gro/tle1 is important for its function during neuronal differentiation. | SIGNOR-129026 |
Q9UBK2 | P49841 | 0 | phosphorylation | down-regulates quantity | 0.476 | GSK3\u03b2 is an important serine/threonine kinase to regulate PPAR\u03b3 coactivator-1\u03b1 degradation. , GSK3\u03b2 reduces PPAR\u03b3 coactivator-1\u03b1 levels by phosphorylating PPAR\u03b3 coactivator-1\u03b1 and subsequently stimulating PPAR\u03b3 coactivator-1\u03b1 degradation by the ubiquitin-proteasomal sys... | SIGNOR-279723 |
Q13094 | P42681 | 0 | phosphorylation | up-regulates | 0.728 | Resting lymphocyte kinase (rlk/txk) targets lymphoid adaptor slp-76 in the cooperative activation of interleukin-2 transcription in t-cells. In this study, we report that rlk phosphorylates slp-76 at its n-terminal yesp/yepp sites. A third tyrosine within the amino-terminal region (y145) appears to be the most importan... | SIGNOR-44669 |
P68400 | P27695 | 1 | phosphorylation | up-regulates activity | 0.485 | Here we demonstrate that APE/Ref-1 is phosphorylated by casein kinase II (CKII). This was shown for both the recombinant APE/Ref-1 protein (Km=0.55 mM) and for APE/Ref-1 expressed in COS cells. Phosphorylation of APE/Ref-1 did not alter the repair activity of the enzyme, whereas it stimulated its redox capability towar... | SIGNOR-250825 |
O14920 | Q13233 | 0 | phosphorylation | up-regulates activity | 0.595 | These results suggested that IKK\u03b2 was a likely substrate for MEKK1 and that MEKK1 phosphorylation of IKK\u03b2 increased its kinase activity. | SIGNOR-279339 |
O43353 | P28482 | 1 | phosphorylation | up-regulates activity | 0.295 | RIP2 directly phosphorylates and activates ERK2 in vivo and in vitro. | SIGNOR-279106 |
Q6ZN28 | P08581 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.394 | Human colon carcinoma SW480 cells express virtually no MACC1. MACC1 cDNA transfection led not only to strong increases in MACC1 mRNA expression (Fig. 3a), but also to a 40-fold upregulation of the HGF receptor MET mRNA expression (Fig. 3b). This was confirmed on the protein level | SIGNOR-266058 |
Q9UHD2 | P11908 | 1 | phosphorylation | up-regulates activity | 0.2 | Here, we show that ionizing radiation results in TBK1-mediated phosphorylation of phosphoribosyl pyrophosphate synthetase (PRPS)1/2 at T228, thereby enhancing PRPS1/2 catalytic activity and promoting deoxyribonucleotide synthesis. | SIGNOR-277317 |
Q8WV28 | P23470 | 0 | dephosphorylation | up-regulates activity | 0.2 | PTPRG activation by the P1-WD peptide affected the tyrosine phosphorylation of several signaling molecules. Data analysis identified 31 molecules whose phosphorylation was modified in a statistically significant manner (Table I). inhibition of ABL1, BMX, BTK, DAB1, ITGB1, JAK2, KDR, KIT, LIMK1, MET, PDGFRB, SHC1, and V... | SIGNOR-254692 |
P61586 | Q9Y3M8 | 0 | gtpase-activating protein | down-regulates activity | 0.645 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260521 |
P63167 | Q13153 | 0 | phosphorylation | down-regulates | 0.378 | Dlc1 phosphorylation on ser(88) by p21-activated kinase 1 (pak1), a signaling nodule, promotes mammalian cell survival by regulating its interaction with bim and the stability of bim. Here we discovered that phosphorylation of ser(88), which juxtapose each other at the interface of the dlc dimer, disrupts dlc1 dimer fo... | SIGNOR-159995 |
P17252 | P10415 | 1 | phosphorylation | up-regulates | 0.349 | Purified pkca can efficiently and directly phosphorylate bcl2 at serine 70 | SIGNOR-60120 |
Q15910 | P31260 | 1 | transcriptional regulation | down-regulates quantity by repression | 0.438 | These data support the proposed regulatory impact of particular PRC2-proteins in expression of HOXA9 and HOXA10 in NK/T-cells. In mammalian cells knockdown of PRC2 components EZH2 or PHF1 led to upregulated HOXA gene expression. | SIGNOR-260070 |
O60610 | P06493 | 0 | phosphorylation | down-regulates activity | 0.2 | In this study, we found that Cdk1 phosphorylated DIAPH1, which inhibited the interaction between DIAPH1 and profilin1 (PFN1) during metaphase.|Thus, the results suggest that the level of cortical F-actin has to be finely maintained by Cdk1 mediated positive and negative regulation of DIAPH1. | SIGNOR-279598 |
P08575 | Q86WV1 | 1 | dephosphorylation | up-regulates activity | 0.361 | Mutational analysis demonstrated the pivotal role of Tyr-232 in SKAP55 in the association with CD45. In Jurkat cells, anti-CD3 antibody stimulation promoted SKAP55 tyrosine phosphorylation and translocation from the cytoplasm to the membrane. Overexpression of SKAP55 in these cells induced transcriptional activation of... | SIGNOR-248360 |
P17252 | P35240 | 1 | phosphorylation | down-regulates activity | 0.328 | PKC\u03b1\nnormally phosphorylates and inactivates NF2.|PKCalpha normally phosphorylates and inactivates NF2. | SIGNOR-280081 |
P10415 | P06493 | 0 | phosphorylation | up-regulates activity | 0.345 | Using synthetic peptides and mutant cell lines, we identified threonine 56, one of two consensus sites for cdc2 within the bcl-2 sequence, as a residue phosphorylated by cdc2. Mutation at threonine 56 abrogated the cell cycle inhibitory effect of bcl-2 without affecting anti-apoptotic function.Taken together, our prese... | SIGNOR-76837 |
P42345 | Q8IYT8 | 1 | phosphorylation | down-regulates | 0.777 | Mtor phosphorylates a mammalian homologue of atg13 and the mammalian atg1 homologues ulk1 and ulk2 | SIGNOR-183961 |
Q86WV6 | Q9BY78 | 0 | ubiquitination | up-regulates activity | 0.2 | As a result, knockdown of RNF26 promoted degradation of MITA after viral infection and prevented degradation of IRF3.|In addition, RNF26 could not induce polyubiquitination of MITA (K150R) in in vitro ubiquitination assays (XREF_FIG). | SIGNOR-278573 |
Q15835 | O00254 | 1 | phosphorylation | down-regulates activity | 0.2 | For example, RhoK phosphorylates and inhibits TIAM1, STEF, and PAR3; disrupts the polarity complex; and prevents Rac activation ( xref ). | SIGNOR-279993 |
Q13887 | P49841 | 0 | phosphorylation | down-regulates | 0.368 | Stability of the klf5 is mediated by proteasomal degradation via phosphorylation by glycogen synthase kinase 3_ (gsk3_) and recognition by f-box and wd repeat domain-containing 7 (fbw7) of a phosphodegron sequence surrounding serine 303 in klf5 | SIGNOR-203627 |
Q14012 | Q9NP62 | 1 | phosphorylation | up-regulates activity | 0.388 | We show that Epac1 and Rap1, in response to cAMP, activate CaMKI to phosphorylate Ser47 in GCM1. This phosphorylation facilitates the interaction between GCM1 and the desumoylating enzyme SENP1 and thereby leads to GCM1 desumoylation and activation. | SIGNOR-262680 |
O60610 | P61586 | 0 | null | up-regulates activity | 0.785 | We find that the small GTPase Rho regulates R-cadherin adherens junction formation via Dia1 (also known as p140mDia) and profilin-1-mediated signaling pathway. The role played by Rho in regulating R-cadherin is underscored by the fact that constitutively active RhoA(Q63L) induces R-cadherin junction formation in MDA-MB... | SIGNOR-253108 |
P60953 | Q70Z35 | 0 | guanine nucleotide exchange factor | up-regulates activity | 0.423 | We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity... | SIGNOR-260572 |
Q13563 | Q9BZL6 | 0 | phosphorylation | up-regulates activity | 0.2 | Here, we report the identification of a previously unrecognized phosphorylation site within the polycystin-2 C terminus (Ser801), and we demonstrate that it is phosphorylated by protein kinase D. These results suggest that growth factor-stimulated, protein kinase D-mediated phosphorylation of polycystin-2 is essential ... | SIGNOR-276284 |
P05771 | Q5JVS0 | 1 | phosphorylation | down-regulates activity | 0.29 | We found a strong phosphorylation of Ki-1/57 by PKCalphabeta, PKCdelta, PKClambda/zeta, and especially by PKCsigma, however not by PKCmi. These data show that Ki-1/57 can serve in principal as a substrate for a wide variety of different PKC isoforms but also that its phosphorylation is strongest with PKCsigma. | This s... | SIGNOR-249247 |
P53350 | Q14980 | 1 | phosphorylation | down-regulates activity | 0.534 | Phosphorylation of NuMA by Plk1 at 1833/34 residue can impact its cortical localization.|These data strongly suggest that Plk1 negatively regulate cortical NuMA localization and that this impact of Plk1 on NuMA is presumably independent of LGN, at least in the anaphase cells. | SIGNOR-279345 |
Q92783 | O60674 | 0 | phosphorylation | up-regulates | 0.617 | Stam is associated with jak3 and jak2 tyrosine kinases via its itam region and phosphorylated by jak3 and jak2 upon stimulation with il-2. | SIGNOR-47834 |
P01100 | Q15418 | 0 | phosphorylation | up-regulates activity | 0.529 | We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kda ribosomal s6 kinase (rsk) and mitogen-activated protein kinase (map kinase), contribute to the serum-induced phosphorylation of c-fos. The major phosphopeptides derived from biosynthetically labeled c-fos corresp... | SIGNOR-37154 |
Q9UKV5 | Q8IWA4 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.309 | Gp78 induces ubiquitylation and proteasomal degradation of Mfn1 and Mfn2. | SIGNOR-272886 |
Q5FBB7 | P51955 | 0 | phosphorylation | up-regulates | 0.2 | Here we show that nek2a phosphorylates human sgo1 and such phosphorylation is essential for faithful chromosome congression in mitosis. phosphorylation sites were mapped to ser(14) and ser(507) | SIGNOR-156882 |
Q16539 | P15336 | 1 | phosphorylation | up-regulates | 0.793 | On the other hand, sapks such as jnks and p38 phosphorylate atf-2 at thr-69, thr-71, and ser-90 which lie close to the n-terminal transcriptional activation domain and stimulate itstrans-activating capacity our results indicate that atf-2 not only directly binds to smad3/4 hetero-oligomers but also that atf-2 is phosph... | SIGNOR-65597 |
P23560 | P16220 | 0 | transcriptional regulation | up-regulates quantity | 0.488 | Brain-derived neurotrophic factor (BDNF) is a critical molecule for learning and memory. Brain BDNF levels correlate with cognitive status. Activation of CREB facilitates the transcription of crucial proteins for activity-dependent plasticity particularly BDNF. | SIGNOR-265062 |
P59595 | P55212 | 0 | cleavage | up-regulates activity | 0.2 | Caspase-6 is activated through the intrinsic pathway and mediates C-terminal cleavage of SARS-CoV N at residues 400 and 403 | SIGNOR-260212 |
Q8TEM1 | P06493 | 0 | phosphorylation | up-regulates activity | 0.549 | In vitro phosphorylation of GST fusion protein containing the carboxyl-terminal domain of gp210 by cyclin B-p34cdc2 protein kinase generates a phosphopeptide that comigrates with a mitosis-specific phosphopeptide. Ser1880 Is the Mitotic Phosphorylation Site of Gp210. | SIGNOR-262699 |
Q16236 | P09601 | 1 | transcriptional regulation | up-regulates quantity by expression | 0.679 | In both models, the inducer-modified and Nrf2-bound Keap1 is inactivated and, consequently, newly synthesized Nrf2 proteins bypass Keap1 and translocate into the nucleus, bind to the ARE and drive the expression of Nrf2 target genes such as NAD(P)H quinone oxidoreductase 1 (NQO1), heme oxygenase 1 (HMOX1), glutamate-cy... | SIGNOR-256276 |
Q9GZY8 | Q15139 | 0 | phosphorylation | up-regulates activity | 0.2 | PKD directly phosphorylates MFF on serines 155, 172, and 275 | SIGNOR-277559 |
P25490 | O14763 | 1 | transcriptional regulation | down-regulates quantity by repression | 0.403 | Depletion of FKBP51 impairs the acetylation status of YY1 and interferes with its binding on the DR5 promoter. The lack of the repressor activity of YY1 increases DR5 transcription and sensitizes melanoma cell to TRAIL-induced apoptosis. | SIGNOR-268793 |
O95551 | Q16659 | 0 | phosphorylation | up-regulates activity | 0.377 | ERK3 phosphorylates TDP2 and promotes its phosphodiesterase activity, thereby upregualting TDP2-mediated DNA damage response and desensitizing lung cancer cells to Top2 inhibitor-induced growth inhibition.|In the current study, we have found that ERK3, an atypical MAPK, phosphorylates TDP2 at S60 and regulates TDP2 's ... | SIGNOR-278245 |
P02788 | O60260 | 0 | ubiquitination | down-regulates activity | 0.2 | We propose that Parkin ubiquitylation of LTF at K649 perturbs LTF\u2019s ability to accumulate intracellular iron levels and that depletion of Parkin, or substitution of K649 on LTF, allows LTF to accumulate intracellular iron levels.|Parkin dependent ubiquitylation of LTF occurred most often on lysines (K) 182 and 649... | SIGNOR-278641 |
O43353 | P98170 | 0 | ubiquitination | up-regulates activity | 0.628 | XIAP is the essential E3 for RIPK2 ubiquitination and interacts with RIPK2 through its baculoviral IAP-repeat (BIR). | SIGNOR-280449 |
P17252 | P02545 | 1 | phosphorylation | up-regulates activity | 0.362 | Mutation of both Ser-403/Ser-404 within a PKC motif flanking the nuclear localization signal inhibits transport of mutant lamin A to the nucleus in 64% of the cells. It is proposed that phosphorylation of the motif in vivo positively regulates nuclear localization together with the nuclear localization sequence. | SIGNOR-248904 |
Q9NQS7 | O14578 | 0 | phosphorylation | up-regulates activity | 0.426 | Figure 5.CIT-K phosphorylates INCENP. (a) Schematic diagram of INCENP structure illustrating the phosphorylated sites identified by MS. | SIGNOR-280232 |
P01106 | Q92995 | 0 | deubiquitination | up-regulates quantity by stabilization | 0.353 | In this study, we demonstrate that the deubiquitinase USP13 stabilizes c-Myc by antagonizing FBXL14-mediated ubiquitination to maintain GSC self-renewal and tumorigenic potential. USP13 was preferentially expressed in GSCs, and its depletion potently inhibited GSC proliferation and tumor growth by promoting c-Myc ubiq... | SIGNOR-274124 |
P49336 | P46531 | 1 | phosphorylation | down-regulates | 0.552 | Purified recombinant cycc:cdk8 phosphorylates the notch icd within the tad and pest domains, and expression of cycc:cdk8 strongly enhances notch icd hyperphosphorylation and pest-dependent degradation by the fbw7/sel10 ubiquitin ligase in vivo. | SIGNOR-130640 |
P49841 | P46531 | 1 | phosphorylation | up-regulates | 0.463 | Here, we observed that gsk3beta was able to bind and phosphorylate notch1ic in vitro, and attenuation of gsk3beta activity reduced phosphorylation of notchic in vivo.Functionally, ligand-activated signaling through the endogenous notch1 receptor was reduced in gsk3beta fibroblasts, implying a positive role for gsk3beta... | SIGNOR-90608 |
Q9BY11 | Q9P286 | 0 | phosphorylation | up-regulates activity | 0.2 | We identified two novel Pak5 substrates, Pacsin1 and Synaptojanin1, proteins that directly interact with one another to regulate synaptic vesicle endocytosis and recycling. Pacsin1 and Synaptojanin1 were phosphorylated by Pak5 and the other group II Paks in vitro, and Pak5 phosphorylation promoted Pacsin1-Synaptojanin1... | SIGNOR-263025 |
O43521 | P31749 | 0 | phosphorylation | down-regulates activity | 0.567 | Recombinant Akt could directly phosphorylate a GST-Bim(EL) fusion protein and identified the Akt phosphorylation site in the Bim(EL) domain as Ser(87). Further, we demonstrated that cytokine stimulation promotes Bim(EL) binding to 14-3-3 proteins. Finally, we show that mutation of Ser(87) dramatically increases the apo... | SIGNOR-252487 |
Q5XPI4 | P19838 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.397 | Here, we identify KPC1 as the Ub ligase (E3) that binds to the ankyrin repeats domain of p105, ubiquitinates it, and mediates its processing both under basal conditions and following signaling. | SIGNOR-272221 |
P03372 | P49841 | 0 | phosphorylation | up-regulates | 0.34 | The gsk-3 inhibitor lithium chloride was used to determine the role of gsk-3 in phosphorylation of ser-102, -104, and -106 and ser-118 in vivo and to explore the role of these serines in the regulation of eralpha function. Treatment of cells with lithium chloride resulted in dephosphorylation of ser-104 and -106 and se... | SIGNOR-139316 |
Q13043 | Q9H2K8 | 0 | phosphorylation | up-regulates | 0.283 | In addition, the thousand-and-one (tao) amino acids kinase or taok13 has been shown to directly phosphorylate and activate hpo or mst1/2 | SIGNOR-192762 |
P16112 | Q99542 | 0 | cleavage | down-regulates quantity by destabilization | 0.4 | Matrix metalloproteinases 19 and 20 cleave aggrecan and cartilage oligomeric matrix protein (COMP)|In this study we investigated the ability of MMP-19 and MMP-20 to cleave two of the macromolecules characterising the cartilage ECM, namely aggrecan and the cartilage oligomeric matrix protein (COMP). Both MMPs hydrolysed... | SIGNOR-266978 |
Q9Y294 | Q00987 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.267 | We found that MDM2 overexpression also decreased the ASF1A half-life time, indicating an accelerated ASF1A degradation.|We next examined whether the presence of RAD6 is essential for MDM2-induced ASF1A ubiquitination. | SIGNOR-278822 |
Q13315 | Q96T60 | 1 | phosphorylation | up-regulates | 0.462 | We demonstrate that pnkp is phosphorylated by the dna-dependent protein kinase (dna-pk) and ataxia-telangiectasia mutated (atm) in vitro. The major phosphorylation site for both kinases was serine 114, with serine 126 being a minor site. Purified pnkp protein with mutation of serines 114 and 126 had decreased dna kinas... | SIGNOR-176008 |
Q01860 | P49137 | 0 | phosphorylation | up-regulates activity | 0.2 | MK2 mediated OCT4 transcriptional activation is a novel mechanism for activating the MYC oncogene in progressive disease neuroblastoma that provides a therapeutic target.|OCT4 phosphorylation at the S111 residue by MK2 was upstream of MYC transcriptional activation. | SIGNOR-279542 |
Q05397 | P04626 | 0 | phosphorylation | up-regulates activity | 0.657 | HER2, EGFR, and additional RTKs directly phosphorylate FAK FERM at Y397.|To further confirm the mechanism of direct FAK activation by HER2, we performed a series of GST pull-down assays with purified recombinant proteins. | SIGNOR-278475 |
P46531 | O00505 | 0 | relocalization | up-regulates | 0.324 | Nicd binds via one of its four potential nuclear localization signals to importins alfa3, alfa4, and alfa7. | SIGNOR-165280 |
P14778 | Q9Y4K3 | 0 | ubiquitination | down-regulates quantity by destabilization | 0.9 | We found that of all TRAFs and E3 ligases examined, TRAF6 preferentially ubiquitinated IL-1R1. | SIGNOR-278576 |
P10645 | P08047 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.2 | Recently, binding of specific protein 1 (Sp1) and cAMP response element binding protein (CREB) to a GC-rich element at -92/-62 has been identified as a critical step in gastrin-dependent regulation of the chromogranin A (CgA) gene in gastric epithelial cells. Here we demonstrate that binding of early growth response pr... | SIGNOR-254273 |
Q13224 | P17252 | 0 | phosphorylation | up-regulates activity | 0.469 | These results indicate that PKC can directly phosphorylate S1303 and S1323 in the NR2B C terminus, leading to enhanced currents through NMDA receptor channels. | SIGNOR-249083 |
P12931 | Q9UGK3 | 1 | phosphorylation | up-regulates activity | 0.406 | To examine this possibility, STAP-2 was co-transfected with constitutively active tyrosine kinases in HEK-293 cells. STAP-2 was strongly phosphorylated by various tyrosine kinases, including v-Src (Fig.2 A-a), a JAK2 tyrosine kinase Tyr-22 and Tyr-322 are the major tyrosine phosphorylation sites by v-Src. | SIGNOR-247337 |
P49959 | P23443 | 0 | phosphorylation | down-regulates quantity by destabilization | 0.2 | MRE11 is highly unstable in PTEN-deficient cells but stability can be significantly restored by inhibiting mTORC1 or p70S6 kinase (p70S6K), downstream kinases whose activities are stimulated by AKT, or by mutating a residue in MRE11 that we show is phosphorylated by p70S6K in vitro. | SIGNOR-265944 |
Q8NG68 | P68363 | 1 | tyrosination | down-regulates | 0.46 | Tubulin tyrosine ligase (ttl) adds a c-terminal tyr to __tubulin as part of a tyrosination/detyrosination cycle present in most eukaryotic cells. / ttl inhibits spontaneous tubulin polymerization | SIGNOR-176915 |
P78527 | O75030 | 1 | phosphorylation | up-regulates activity | 0.2 | These results suggest that DNA-PK can target MITF-S325 for phosphorylation. In melanocytes and melanoma cells, MITF is rapidly phosphorylated by DNA-PK and interacts with NBS1–RAD50 but not MRE11, destabilizing the MRN complex. | SIGNOR-277899 |
Q9Y397 | P01111 | 1 | palmitoylation | up-regulates activity | 0.404 | Covalent lipid modifications mediate the membrane attachment and biological activity of Ras proteins. All Ras isoforms are farnesylated and carboxyl-methylated at the terminal cysteine; H-Ras and N-Ras are further modified by palmitoylation. Here we report that H- and N-Ras are palmitoylated by a human protein palmito... | SIGNOR-261355 |
P10912 | P18031 | 0 | dephosphorylation | down-regulates activity | 0.501 | PTPH1 only bound Tyr534, whereas PTP1B and TC-PTP bound multiple phosphopeptides. Earlier work suggests that Tyr332, Tyr487, Tyr534, Tyr566, and Tyr627 are all phosphorylated after GH stimulation (21). Apart from Tyr627, all of these also appear good PTP substrates | SIGNOR-248420 |
P00742 | P02749 | 1 | cleavage | down-regulates activity | 0.386 | In the previous study, we found that factor Xa can produce the nicked form by cleaving Lys 317-Thr 318, using recombinant human domain V (r-Domain V). |The cleavage was completely inhibited by plasmin inhibitor (alpha2PI). The nicked form was demonstrated to show reduced affinity for CL with a dissociation constant of ... | SIGNOR-266997 |
Q8NBL1 | P46531 | 1 | glycosylation | up-regulates | 0.606 | O-glucosylation of epidermal growth factor-like (egf) repeats in the extracellular domain of notch is essential for notch function. A udp-glucose:protein o-glucosyltransferase (poglut/rumi) transfers o-glucose to serine within the o-glucose consensus. | SIGNOR-198713 |
P61978 | P53779 | 0 | phosphorylation | up-regulates activity | 0.339 | JNK Phosphorylation of HnRNP K Increases Its Transcriptional Activity. the primary site for JNK phosphorylation consists of serines 216 and 353 on the K protein. | SIGNOR-250083 |
P45983 | P01106 | 1 | phosphorylation | up-regulates activity | 0.556 | The jnk pathway is selectively involved in the c-myc-mediated apoptosis and that the apoptotic function of c-myc is directly regulated by jnk pathway through phosphorylation at ser-62 and ser-71. | SIGNOR-236018 |
P20823 | Q9Y463 | 0 | phosphorylation | up-regulates | 0.502 | Mirk phosphorylates hnf1 at amino acid 249mkk3 enhanced mirk kinase activity and the transcriptional activation of hnf1alpha by mirk | SIGNOR-86728 |
Q9BUB5 | P06730 | 1 | phosphorylation | up-regulates | 0.779 | Mnk1 and mnk2 regulate protein synthesis by phosphorylating the initiation factor eif4e. | SIGNOR-166646 |
Q16584 | P24941 | 0 | phosphorylation | up-regulates activity | 0.2 | Using in vitro kinase assays and phosphomutants, we determined that CDK1 phosphorylates MLK3 on Ser548 and decreases MLK3 activity during mitosis, whereas CDK2 phosphorylates MLK3 on Ser770 and increases MLK3 activity during G1/S and G2 phases. | SIGNOR-277604 |
Q02156 | P21802 | 1 | phosphorylation | up-regulates | 0.2 | Phosphorylation of serine 779 in fibroblast growth factor receptor 1 and 2 by protein kinase c(epsilon) regulates ras/mitogen-activated protein kinase signaling and neuronal differentiation | SIGNOR-201675 |
Q13363 | O95471 | 1 | transcriptional regulation | down-regulates quantity by repression | 0.2 | ChIP assays revealed that SNAI1P is recruited on the CLDN7 gene promoter along with the co-repressor CtBP1 and the effector HDAC1. | SIGNOR-254105 |
O43248 | O15550 | 0 | transcriptional regulation | up-regulates quantity by expression | 0.302 | Evidence for direct involvement of UTX in regulation of HOX gene activity was demonstrated through UTX knockdown experiments in HEK293T cells in which loss of UTX induced transcriptional repression of HOXA and HOXC clusters. | SIGNOR-260026 |
Q96BR1 | Q66PJ3 | 1 | phosphorylation | down-regulates activity | 0.2 | AIP4 is phosphorylated by CISK in vitro on WW domain residues, which may impact its ability to interact with and ubiquitinate substrate proteins.|Expression of a constitutively active CISK inhibits CXCR4 degradation, possibly by attenuating CXCR4 binding to and ubiquitination by AIP4 and/or modulating the action of AIP... | SIGNOR-280124 |
O95644 | P16298 | 0 | relocalization | up-regulates | 0.724 | The ca2+ dependent phosphatase calcineurin induces cardiac and skeletal muscle hypertrophy by a process that involves nf-at nuclear translocation, and activation of mef2c. | SIGNOR-84047 |
Q9HCE7 | Q13485 | 1 | ubiquitination | down-regulates activity | 0.745 | Smurfs, which otherwise cannot directly bind to smad4, mediated poly-ubiquitination of smad4 in the presence of smad6 or smad7. Smad signaling is negatively regulated by inhibitory (i) smads and ubiquitin-mediated processes. | SIGNOR-236096 |
P35790 | P35968 | 1 | phosphorylation | down-regulates quantity by destabilization | 0.249 | Here, we show for the first time a possible mechanism by which CKI dependent phosphorylation of VEGFR2 at specific sites in its C-terminal tail triggers SCF beta-TRCP -mediated VEGFR2 ubiquitination and destruction. | SIGNOR-279029 |
O00192 | Q9UDY2 | 0 | relocalization | down-regulates activity | 0.369 | We identified ARVCF as a binding partner of ZO-1 and ZO-2 and characterized the role of PDZ-domain proteins in plasma membrane and nuclear localization of ARVCF. ZO-2, in contrast, relocated to the nucleus with ARVCF, and, given the interaction between the ZO-2 PDZ domains and ARVCF, raised the possibility that ZO-2 ma... | SIGNOR-252122 |
P31749 | A8MYZ6 | 1 | phosphorylation | down-regulates | 0.648 | The phosphorylation of the two remaining akt-dependent sites inhibits foxo6 transcriptional activity | SIGNOR-252582 |
Q9Y5K5 | Q15796 | 1 | deubiquitination | up-regulates | 0.378 | Here, we report a novel interaction between smads and ubiquitin c-terminal hydrolase uch37, a deubiquitinating enzyme that could potentially reverse smurf-mediated ubiquitination. In gst pull down experiments, uch37 bound weakly to smad2 and smad3, and bound very strongly to smad7 in a region that is distinct from the ... | SIGNOR-138876 |
Q13490 | Q86VP3 | 1 | polyubiquitination | down-regulates quantity by destabilization | 0.303 | Under basal conditions, PACS-2 underwent K48-linked poly-ubiquitination, resulting in PACS-2 proteasomal degradation. Biochemical assays showed cIAP-1 and cIAP-2 interacted with PACS-2 in vitro and co-immunoprecipitation studies demonstrated that the two cIAPs bound PACS-2 in vivo. More importantly, both cIAP-1 and cIA... | SIGNOR-272851 |
P36956 | P53350 | 0 | phosphorylation | up-regulates activity | 0.441 | As illustrated in , the turnover of nuclear SREBP1a was attenuated in the presence of Plk1, confirming that Plk1 stabilizes nuclear SREBP1 by reducing its degradation.|Figure 2.Plk1 phosphorylates threonine 424, serine 467 and serine 486 in nuclear SREBP1 during mitosis. (A) In vitro kinase assay with recombinant nucle... | SIGNOR-279382 |
Q9UBK2 | Q96EB6 | 0 | deacetylation | up-regulates activity | 0.799 | SIRT1 overexpression reduces muscle wasting by blocking the activation of FoxO1 and 3 SIRT1 activation has been reported to increase dramatically endurance exercise through the activation of PGC-1_ in muscle, which stimulates fatty acid oxidation | SIGNOR-217963 |
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