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P00533
Q16539
0
phosphorylation
down-regulates
0.508
In conclusion, the use of pharmacological agents suggests that p38 mapk is the enzyme involved in egfr phosphorylation, as well as internalization, following exposure of cells to various stress-inducing conditions.
SIGNOR-149089
P31749
P62714
0
dephosphorylation
down-regulates
0.508
These results confirm that the activity changes observed are achieved by a reversible phosphorylation mechanism, and also argue that pp2a may negatively regulate rac-pk activity in vivo. Dephosphorylation of the activated rac-pk in itro by pp2ac resulted in an 87% reduction of kinase activity
SIGNOR-252636
P13807
P17612
0
phosphorylation
down-regulates activity
0.508
The results presented in this paper show that the phosphorylation of glycogen synthetase a by cyclic AMP-dependent protein kinase results in the phosphorylation of two distinct serines termed site-l and site-2, which account for 90% of the total phosphorylation
SIGNOR-253009
Q9HAW4
Q9Y2K6
0
deubiquitination
up-regulates quantity by stabilization
0.508
USP20 deubiquitinates and stabilizes Claspin.
SIGNOR-272821
O95071
P62837
0
ubiquitination
up-regulates activity
0.508
Using an in vitro reconstitution, specific E2 (ubiquitin-conjugating) enzymes (human UbcH4, UbcH5B, and UbcH5C) transferred ubiquitin molecules to hHYD, leading to the ubiquitination of TopBP1. TopBP1 was usually ubiquitinated and degraded by the proteosome, whereas X-irradiation diminished the ubiquitination of TopBP1...
SIGNOR-272668
P04083
P00533
0
phosphorylation
up-regulates
0.508
The authors identified several phosphorylated residues by a combination of peptide mapping and sequence analysis and showed that recombinant pp60c-src phosphorylates annexin a1 near its amino terminus, at tyrosine 21 (tyr21). Also polyoma virus middle t/pp60c-src complex, recombinant pp50v-abl, and the egf receptor/kin...
SIGNOR-202776
P13807
Q96RG2
0
phosphorylation
down-regulates activity
0.508
Recombinant human PASK (hPASK) phosphorylates purified muscle glycogen synthase, causing robust inactivation. Furthermore, hPASK interacts directly with glycogen synthase when expressed in cultured cells and this interaction and the phosphorylation of glycogen synthase by human PASK (hPASK) are inhibited by glycogen.
SIGNOR-245866
Q9UEW8
Q96J92
0
phosphorylation
up-regulates activity
0.507
Vitari et al. (76) and Moriguchi et al. (52) demonstrated that WNK4 bound and phosphorylated PASK at Thr-233 and Ser-373 in mammalian cells.| this phosphorylation event activates PASK, which in turn phosphorylates and activates NKCC1
SIGNOR-264641
P49137
P28482
0
phosphorylation
up-regulates
0.507
Using novel methodology we demonstrate that activation of mapkap kinase-2 requires the phosphorylation of any two of the three residues thr222, ser272 and thr334. gst-mapkap kinase-2 lacking the n-terminal domain was inactive, but activated fully when phosphorylated at thr222, ser272 and thr334 by p42 mapk or rk.
SIGNOR-44343
P04183
P06493
0
phosphorylation
down-regulates
0.507
Given that the dimeric form of tk1 is less active than the tetrameric, we propose that mitotic phosphorylation of serine-13 is of physiological importance, in that it may counteract atp-dependent activation of tk1 by affecting its quaternary structure, thus attenuating its enzymatic function at the g2/m phase.
SIGNOR-95574
P10415
P00441
0
binding
up-regulates activity
0.507
Familial amyotrophic lateral sclerosis (ALS)-linked mutations in the copper-zinc superoxide dismutase (SOD1) gene cause motor neuron death in about 3% of ALS cases. While the wild-type (wt) protein is anti-apoptotic, mutant SOD1 promotes apoptosis.|We now demonstrate that both wt and mutant SOD1 bind the anti-apoptotic...
SIGNOR-262799
Q96GD4
Q9UJP4
0
binding
up-regulates activity
0.507
KLHL21 directly binds to Aurora B and mediates ubiquitination of Aurora B in vitro. In contrast to KLHL9 and KLHL13, KLHL21 localizes to midzone microtubules in anaphase and recruits Aurora B and Cul3 to this region. Together, our results suggest that different Cul3 adaptors nonredundantly regulate Aurora B during mito...
SIGNOR-271848
P12931
P63092
0
binding
up-regulates activity
0.507
Here we demonstrate that Galphas and Galphai, but neither Galphaq, Galpha12 nor Gbetay, directly stimulate the kinase activity of downregulated c-Src
SIGNOR-256527
Q9GZV5
Q9UDY2
0
binding
down-regulates
0.507
In addition, yap and taz interact with another tight junction protein zo-2, which was reported to increase nuclear localization of yap and tight-junction localization of taz.
SIGNOR-175931
Q04726
P98170
0
ubiquitination
up-regulates activity
0.507
These findings suggest that TLE3 ubiquitylation by XIAP may be required during Wnt pathway activation to facilitate its dissociation from TCF/Lef, allowing subsequent beta-catenin binding and transcriptional activation.
SIGNOR-278528
Q13547
P00519
0
phosphorylation
up-regulates activity
0.507
Despite the fact that HDAC1 was phosphorylated by co-expression with c-Abl, stabilization of HDAC1 by c-Abl was not affected by mutations in its sites phosphorylated by c-Abl.|c-Abl induces stabilization of histone deacetylase 1 (HDAC1) in a kinase activity dependent manner.
SIGNOR-280169
O14974
O60285
0
phosphorylation
down-regulates
0.507
Phosphorylation of ser(445), ser(472), and ser(910) of mypt1 by nuak1 promoted the interaction of mypt1 with 14-3-3 adaptor proteins, thereby suppressing phosphatase activity.
SIGNOR-164747
Q92667
O43255
0
polyubiquitination
down-regulates quantity by destabilization
0.507
Seven In-Absentia Homolog 2 (Siah2), an E3-ubiquitin ligase whose expression is induced in hypoxic conditions, formed a complex and degraded AKAP121.
SIGNOR-272641
P22891
P38435
0
carboxylation
up-regulates activity
0.507
Gamma-carboxylation is essential in the activation and proper functioning of multiple VK-dependent proteins (VKDP), the most well-known of which are involved in blood clotting, including coagulation factors (FII, FVII, FIX and FX) and natural anti-clotting agents (protein C, protein S (ProS; OMIM*176880) and protein Z
SIGNOR-265926
P42224
Q13555
0
phosphorylation
up-regulates activity
0.507
For maximal gene activation, S727 in the transcription activation domain of Stat1 also is inducibly phosphorylated by IFN-gamma. We previously purified a group of nuclear proteins that interact specifically with the Stat1 transcription activation domain. In this report, we identified one of them as the multifunctional ...
SIGNOR-250706
O60934
P24941
0
phosphorylation
up-regulates activity
0.507
Nbs1 is phosphorylated by Cdk2 on Ser432 in human whole-cell extracts.
SIGNOR-279500
P30305
Q8TDC3
0
phosphorylation
down-regulates
0.506
Hssad1 specifically phosphorylated wee1a, cdc25-c, and -b on ser-642, ser-216, and ser-361 in vitro, respectivelyphosphorylation of cdc25 triggers cell-cycle arrest by the sequestration of cdc25 by 14-3-3
SIGNOR-124839
P32004
Q15418
0
phosphorylation
up-regulates activity
0.506
Western blot analysis demonstrated that the L1 kinase activity from PC12 cells that phosphorylated this site was co-eluted with the S6 kinase, p90(rsk). Moreover, S6 kinase activity and p90(rsk) immunoreactivity co-immunoprecipitate with L1 from brain, and metabolic labeling studies have demonstrated that Ser1152 is ph...
SIGNOR-248948
P28482
P24723
0
phosphorylation
up-regulates activity
0.506
Protein kinase C-eta regulates Mcl-1 level via ERK1. knockdown of PKCη but not PKCα, -δ or -ε caused a significant decrease in ERK (extracellular signal-regulated kinase) phosphorylation. Knockdown of ERK1 but not ERK2 decreased Mcl-1 level, and the decrease in Mcl-1 caused by PKCη knockdown was restored by ERK1 overe...
SIGNOR-261910
P13569
Q13976
0
phosphorylation
up-regulates
0.506
Direct amino acid sequencing and peptide mapping of cf-2 revealed that serines 660, 700, 737, and 813 as well as serine 768, serine 795, or both were phosphorylated by pka and pkgcftr possesses a large cluster of strict dibasic consensus sites for phosphorylation by protein kinase a (pka) in the r-domain and an obligat...
SIGNOR-18249
Q12778
Q13627
0
phosphorylation
down-regulates
0.506
Additionally, ck1, dyrk1a, and cdk2 also phosphorylate foxos at various sites to inhibit foxos activity.
SIGNOR-183670
Q12982
Q4KMG0
0
binding
up-regulates activity
0.506
Bnip-2 and jlp are brought together through mutual interaction with cdo. the cdo-bnip-2 interaction stimulates cdc42 activity, which in turn promotes p38alpha/beta activity and cell differentiation.
SIGNOR-179864
P56945
P51813
0
phosphorylation
up-regulates quantity
0.506
Recombinant Bmx kinase was found to effectively phosphorylate the wt CAS SH3 domain on Tyr-12 (Figure 2B). A novel phosphorylation site on CAS, Tyr-12 (Y12) within the ligand-binding hydrophobic pocket of the CAS SH3 domain, was identified and found to be enriched in Src-transformed cells and invasive human carcinoma c...
SIGNOR-276384
Q02763
O95841
0
binding
up-regulates
0.506
In experiments with human endothelial cell lines, ang3 was identified as an antagonist of tie2 and ang4 was identified as an agonist of tie2.
SIGNOR-127354
P63092
Q03431
0
binding
up-regulates activity
0.506
This calciotropic hormone exerts its actions via binding to the PTH/PTH-related peptide receptor (PTH1R), which couples to multiple heterotrimeric G proteins, including Gs and Gq/11.
SIGNOR-270551
Q969H4
P12931
0
phosphorylation
up-regulates activity
0.506
We identified Tyr 26 as a PDGF-induced and, additionally, Tyr 519 and Tyr 665 as SRC-induced tyrosine phosphorylation sites. Phosphomimetic mutants indicate that phosphorylation of Tyr 519 recruits CNK1 to the nucleus and additional phosphorylation of Tyr 26 enables CNK1 to promote SRE-dependent gene expression.
SIGNOR-275918
Q13588
P29353
0
binding
up-regulates
0.506
T cell activation effects an increase in grap association with p36/38, shc, sos, and dynamin.
SIGNOR-45528
Q13950
Q9GZV5
0
binding
up-regulates
0.506
Taz binding to the transcription factor runx2 promotes osteoblast lineage specification, whereas taz binding to the transcription factor ppargamma inhibits adipogenesis.
SIGNOR-195218
P63092
P35368
0
binding
up-regulates activity
0.506
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256807
P67809
P51608
0
binding
up-regulates activity
0.506
In this study, we show that MeCP2 interacts with the RNA-binding protein Y box-binding protein 1 and regulates splicing of reporter minigenes. Importantly, we found aberrant alternative splicing patterns in a mouse model of RTT. Thus, we uncovered a previously uncharacterized function of MeCP2 that involves regulation ...
SIGNOR-277700
Q9NZ52
Q9P253
0
monoubiquitination
down-regulates activity
0.506
Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability. By in vitro ubiquitylation assays, we have identified lysine 258 in the GAT domain as a major ubiquitylation site that resides adjacent to the ubiquitin-binding site. Furthermore, the GAT domain ubiquitylated by hVPS18 no longer binds to ubiq...
SIGNOR-271610
Q02078
Q00535
0
phosphorylation
down-regulates activity
0.506
Cdk5-mediated inhibition of the protective effects of transcription factor mef2 in neurotoxicity-induced apoptosis.We have identified the prosurvival transcription factor mef2 as a direct nuclear target of cdk5. Cdk5 phosphorylates mef2 at a distinct serine in its transactivation domain to inhibit mef2 activity.
SIGNOR-100574
O15105
Q96J02
0
ubiquitination
down-regulates
0.506
We identified atrophin 1-interacting protein 4 (aip4) as an e3 ubiquitin ligase that specifically targets smad7 for ubiquitin-dependent degradation without affecting the turnover of the activated tbetari. Surprisingly, we found that despite the ability to degrade smad7, aip4 can inhibit tgf-beta signaling, presumably b...
SIGNOR-137951
Q6ZMZ0
Q96LR5
0
binding
up-regulates activity
0.506
We demonstrated that both UbcH7 and UbcH8 bind to full-length NKLAM.  We demonstrated decreased protein expression and enhanced ubiquitination of URKL-1 in the presence of NKLAM. These data indicate that NKLAM is a RING finger protein that binds Ubcs and
SIGNOR-271592
P62140
Q96A00
0
binding
down-regulates activity
0.505
We conclude that ILK may activate smooth-muscle contraction both directly, via phosphorylation of myosin, and indirectly, via phosphorylation and activation of CPI-17 and PHI-1, leading to inhibition of MLCP.|CPI-17 and PHI-1 thiophosphorylated by ILK at Thr(38) or Thr(57) respectively inhibited myosin light-chain phos...
SIGNOR-265742
Q8WUF5
P06493
0
phosphorylation
up-regulates activity
0.505
Cyclin B/cyclin-dependent kinase 1 (CDK1) phosphorylates inhibitor of apoptosis stimulating protein of P53 (iASPP) to promote iASPP nucleus localization and its inhibitory effect on p53. 
SIGNOR-273585
P29474
P0DP24
0
binding
up-regulates activity
0.505
Electrons flow from the C-terminal reductase domain of one NOS monomer to the N-terminal oxygenase domain of the other NOS monomer (Siddhanta et al., 1998). The primary mode of enzyme activation is the binding of calcium-bound calmodulin to the N-terminal CaM-binding domain. This facilitates a structure change and the ...
SIGNOR-266323
O95944
Q8IZD2-8
0
binding
up-regulates activity
0.505
We identify natural cytotoxicity receptor NKp44 (NKp44L), a novel isoform of the mixed-lineage leukemia-5 protein, as a cellular ligand for NKp44. Unlike the other MLL family members, NKp44L is excluded from the nucleus, but expressed at the cell-surface level; its subcellular localization is being associated with the ...
SIGNOR-260042
Q8TD16
P49792
0
relocalization
up-regulates quantity
0.505
We show that the dynein/dynactin adaptor BICD2 is specifically recruited to the NPC in G2phase through a direct interaction with the NPC componentRanBP2.
SIGNOR-259122
Q9Y5X1
Q07912
0
phosphorylation
up-regulates
0.505
We have previously shown that sh3px1, phosphorylated by ack2 (activated cdc42-associated tyrosine kinase 2), regulates the degradation of egf (epidermal growth factor) receptor.
SIGNOR-142569
P35222
Q96EB6
0
deacetylation
up-regulates quantity
0.505
SIRT1 deacetylates β-catenin to promote its accumulation in the nucleus and thus induces the transcription of genes that block MSC adipogenesis.
SIGNOR-256208
O43426
Q00535
0
phosphorylation
down-regulates activity
0.505
Cdk5 phosphorylation inhibited the inositol 5-phosphatase activity of synaptojanin 1, whereas dephosphorylation by calcineurin stimulated such activity.|The activity of synaptojanin 1 was also stimulated by its interaction with endophilin 1, its major binding partner at the synapse. Notably, Cdk5 phosphorylated serine ...
SIGNOR-279685
P36897
Q9Y5K5
0
binding
up-regulates quantity by stabilization
0.505
Smad7 can act as an adaptor able to recruit uch37 to the type i tgf-beta receptor. Consequently, uch37 dramatically up-regulates tgf-beta-dependent gene expression by de-ubiquitinating and stabilizing the type i tgf-beta receptor.
SIGNOR-150135
P16615
O00631
0
binding
down-regulates activity
0.505
The structure suggests a mechanism for selective Ca2+ loading and activation of SERCA, and provides new insight into how SLN and PLB inhibition arises from stabilization of this E1 intermediate state without bound Ca2+.
SIGNOR-264779
Q96SN8
Q38SD2
0
phosphorylation
up-regulates activity
0.505
Interestingly, LRRK1 in turn phosphorylates CDK5RAP2(Cep215), a human homologue of Drosophila Centrosomin (Cnn), in its gamma-tubulin-binding motif, thus promoting the interaction of CDK5RAP2 with gamma-tubulin. LRRK1 phosphorylation of CDK5RAP2 Ser 140 is necessary for CDK5RAP2-dependent microtubule nucleation.
SIGNOR-275468
P01266
P07202
0
catalytic activity
up-regulates activity
0.505
After transport through the apical membrane, I− is covalently bound to the tyrosyl residues of Tg by thyroid peroxidase (TPO).
SIGNOR-259914
Q13263
Q9UBF1
0
binding
up-regulates activity
0.505
In this study, we demonstrated that the tripartite motif-containing protein 28 (TRIM28) binds directly to and promotes FBP1 for ubiquitination and degradation. MAGE-A3 and MAGE-C2, which are known to be overexpressed in HCC, can enhance TRIM28-dependent degradation of FBP1 by forming ubiquitin ligase complexes with TRI...
SIGNOR-267593
P46734
O43318
0
phosphorylation
up-regulates activity
0.505
Taken together, our data indicate that TAK1 and TAB1 play a pivotal role as upstream signal transducers activating the MKK3-p38 MAPK signaling cascade that leads to the induction of type I collagen expression by TGF-beta(1). In addition, our findings also suggest that TAK1 has a novel function in regulation of the stea...
SIGNOR-42402
O95944
Q8IZD2
0
binding
up-regulates activity
0.505
We identify natural cytotoxicity receptor NKp44 (NKp44L), a novel isoform of the mixed-lineage leukemia-5 protein, as a cellular ligand for NKp44. Unlike the other MLL family members, NKp44L is excluded from the nucleus, but expressed at the cell-surface level; its subcellular localization is being associated with the ...
SIGNOR-260045
Q96FE5
P23515
0
binding
up-regulates
0.505
Nogo-a, myelin-associated glycoprotein (mag), and oligodendrocyte myelin glycoprotein (omgp)...signal through a common receptor complex in neurons, which includes the ligand binding nogo-66 receptor (ngr), and two signal-transducing binding partners, p75 and lingo-1...
SIGNOR-133640
Q15691
P48730
0
phosphorylation
up-regulates activity
0.505
We further show that casein kinase 1\u03b4 binds and phosphorylates EB1 and promotes microtubule growth.
SIGNOR-279165
Q9Y210
P06241
0
phosphorylation
up-regulates activity
0.505
Fyn phosphorylates TRPC6 and increases its diacylglycerol stimulated single channel activity.
SIGNOR-279717
P41968
Q96G30
0
binding
down-regulates activity
0.505
We report that MRAP and MRAP2 can interact with all 5 MCRs. This interaction results in MC2R surface expression and signaling. In contrast, MRAP and MRAP2 can reduce MC1R, MC3R, MC4R, and MC5R responsiveness to [Nle4,D-Phe7]alpha-melanocyte-stimulating hormone (NDP-MSH). MRAP and MRAP2 can reduce the surface expression...
SIGNOR-252367
P27986
P35968
0
binding
up-regulates activity
0.505
null
SIGNOR-261917
P06400
P67775
0
dephosphorylation
up-regulates
0.505
This dephosphorylation returns prb to its active, growth suppressive state.
SIGNOR-75398
P29803
Q15118
0
phosphorylation
down-regulates
0.505
Human pdh1 and rat pdh2 were expressed previously and were shown to have different specific activities and the ability to be phosphorylated by pdk1 and pdk2
SIGNOR-143966
O60240
P17612
0
phosphorylation
down-regulates activity
0.504
PKA increased lipolysis in cells expressing Peri A because it abrogated the inhibitory actions of Peri A on lipolysis.‚  amino-terminal PKA sites (Ser-81, Ser-222, and Ser-276)
SIGNOR-250028
Q13263
Q9H4L7
0
binding
up-regulates activity
0.504
SMARCAD1 interacts with HDAC1 and KAP1 and is required for their binding to heterochromatin
SIGNOR-239838
P55211
O15392
0
binding
down-regulates
0.504
Survivin (an inhibitor of apoptosis) phosphorylation on thr34 may regulate apoptosis at cell division via an interaction with caspase-9.
SIGNOR-84065
P18754
P06493
0
phosphorylation
up-regulates activity
0.504
We show here that Cdc2 kinase phosphorylates the serines located in or near the nuclear localization signal (NLS) of hum an RCC1, the nucleotide exchange factor for Ran. This phosphorylation is necessary for RCC1 to generate RanGTP on mitotic chromosomes in mammalian cells, which in turn is required for spindle assembl...
SIGNOR-262704
P61586
Q9P2N2
0
gtpase-activating protein
down-regulates activity
0.504
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity...
SIGNOR-260483
Q15797
P49841
0
phosphorylation
down-regulates
0.504
Phosphorylation at the gsk3 sites represses the transcriptional activity of smad1 by enhancing proteasomal degradation of psmad1cter
SIGNOR-159484
P32245
Q96G30
0
binding
down-regulates activity
0.504
We report that MRAP and MRAP2 can interact with all 5 MCRs. This interaction results in MC2R surface expression and signaling. In contrast, MRAP and MRAP2 can reduce MC1R, MC3R, MC4R, and MC5R responsiveness to [Nle4,D-Phe7]alpha-melanocyte-stimulating hormone (NDP-MSH). MRAP and MRAP2 can reduce the surface expression...
SIGNOR-252363
Q13950
Q9HCE7
0
ubiquitination
down-regulates activity
0.504
Recently we have found that smurf1 mediates the protein degradation of the osteoblast-specific transcription factor runx2/cbfa1.
SIGNOR-95233
P50148
P50052
0
binding
up-regulates
0.504
These neuropeptide gpcrs are coupled to the activation of phospholipase c, and therefore to calcium ele- vation and protein kinase c (pkc) activation, through g proteins of the alfaq family
SIGNOR-106995
P63096
P21453
0
binding
up-regulates activity
0.504
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256713
Q6JBY9
P53778
0
phosphorylation
down-regulates activity
0.504
Peptide T2 was sequenced and shown to comprise residues 79–112 of CapZIP, phosphorylated at Ser-108 (Figure 2B). The identity of peptide T1 is unknown. These experiments established that the SAPK3/p38γ substrate was CapZIP. Using this antibody, we showed by immunoblotting that bacterially expressed CapZIP was phosphory...
SIGNOR-263083
P63096
P31391
0
binding
up-regulates activity
0.504
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256680
Q9UD71
P17612
0
phosphorylation
up-regulates activity
0.504
DARPP-32 (dopamine and cyclic AMP-regulated phospho-protein, relative molecular mass 32,000) is converted into an inhibitor of protein phosphatase 1 when it is phosphorylated by protein kinase A (PKA) at threonine 34.‚ 
SIGNOR-250031
P01106
P68400
0
phosphorylation
down-regulates quantity by destabilization
0.504
 Together, our findings provide evidence for CK1α-mediated destruction of c-Myc and identify c-Myc S252 as a crucial CK1α phosphorylation site for c-Myc degradation.
SIGNOR-276388
P08754
P21453
0
binding
up-regulates activity
0.504
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-256856
P31350
P06493
0
phosphorylation
down-regulates
0.504
We found that, during g2, following cdk-mediated phosphorylation of thr33, rrm2 is degraded via scf(cyclin f) to maintain balanced dntp pools and genome stability.
SIGNOR-197630
P29474
P31751
0
phosphorylation
up-regulates activity
0.504
The phosphorylation of both S617 and S635 have also been shown to promote increased eNOS-derived NO release (Michell et al., 2002). The phosphorylaiton of S617 can be induced by PKA or Akt activity, and may serve to sensitize eNOS to calmodulin binding and modulate the phosphorylation of other eNOS sites
SIGNOR-251624
P60484
Q96KB5
0
phosphorylation
down-regulates activity
0.504
PTEN is phosphorylated by TOPK and is required for mitotic entry. In addition, reduced PTEN phosphorylation levels upon TOPK knockdown correlated with decreased Akt activation (Fig. 4e) suggesting that TOPK mediated phosphorylation may lead to PTEN inactivation. By using various PTEN mutants in a kinase assay we concl...
SIGNOR-271472
Q93074
P10071
0
binding
down-regulates
0.504
We propose that activated gli3 physically targets med12 in mediator to reverse mediator-dependent suppression of shh target gene (i.e., Gli1 or cyclin d1) transcription.
SIGNOR-149876
Q5JZY3
Q15375
0
phosphorylation
up-regulates activity
0.504
By using co-immunoprecipitation, we demonstrated physical interaction between kinase-deficient EPHA10 with kinase-sufficient EPHA7 receptor. we speculate that the kinase activity of EPHA7 cross-phosphorylates EPHA10.
SIGNOR-273873
O14981
Q14919
0
binding
up-regulates activity
0.504
We present evidence that the NC2alpha subunit interacts with BTAF1. Addition of NC2alpha or the NC2 complex can stimulate the ability of BTAF1 to interact with TBP.
SIGNOR-263918
O43561
P18031
0
dephosphorylation
down-regulates activity
0.504
Using a pharmacological inhibitor, we provide evidence that PTP1B activation and LAT dephosphorylation processes were required for irreversible platelet aggregation.|In collagen-stimulated platelets, the signaling complexes recruited by tyrosine-phosphorylated LAT are essential for PLCgamma2 activation
SIGNOR-248403
P62979
Q93008
0
cleavage
up-regulates quantity
0.504
Here we provide data suggesting that two of the four mammalian ubiquitin precursors, UBA52 and UBA80, are processed mostly post-translationally whereas the other two, UBB and UBC, probably undergo a combination of co- and post-translational processing. Using an unbiased biochemical approach we found that UCHL3, USP9X, ...
SIGNOR-270826
P63000
Q12979
0
gtpase-activating protein
down-regulates activity
0.504
We therefore developed a screening-compatible live-cell imaging assay, using FRET-based biosensors for the prototype GTPases RHOA, RAC1 and CDC4215,19,20 (Extended Data Fig. 2 and Supplementary Note 1)|We found catalytic activities for 45/75 RhoGEFs and 48/63 RhoGAPs| Our data thus not only reveal extensive promiscuity...
SIGNOR-260525
P98170
P04049
0
phosphorylation
up-regulates activity
0.504
Interaction and stabilization of X-linked inhibitor of apoptosis by Raf-1 protein kinase.|We also demonstrate that Raf-1 phosphorylates XIAP in vitro and in vivo.
SIGNOR-279105
O75419
Q8TEA8
0
binding
up-regulates activity
0.504
The DNA unwinding element (DUE)-binding protein (DUE-B) binds to replication origins coordinately with the minichromosome maintenance (MCM) helicase and the helicase activator Cdc45 in vivo, and loads Cdc45 onto chromatin in Xenopus egg extracts. In egg extracts alanine mutation of the DUE-B C-terminal phosphorylation ...
SIGNOR-273973
Q02779
Q9UQF2
0
binding
up-regulates
0.504
The c-jun nh2-terminal kinase (jnk)-interacting protein (jip) group of scaffold proteins (jip1, jip2, and jip3) can interact with components of the jnk signaling pathway and potently activate jnk.
SIGNOR-134552
P08603
Q03591
0
binding
down-regulates activity
0.504
Finally, we have been able to establish that CFHR1 can sterically inhibit the interaction that CFH/CFHL-1 SCR1-4 makes with C3b.|CFH regulates the alternative pathway of complement in both the fluid phase and on self-surfaces: It competes with complement factor B (CFB) for binding to C3b and C3(H2O) thereby blocking th...
SIGNOR-263476
Q9NPH3
Q01638
0
binding
up-regulates activity
0.504
The initial step in IL-33 signal transduction is ligand-induced conformational changes in IL-33R, which facilitate recruitment of interleukin-1 receptor accessory protein (IL-1RAP).
SIGNOR-277715
Q9UJY5
P68400
0
phosphorylation
down-regulates activity
0.504
Serine-355 of GGA1 is phosphorylated in vivo and in vitro. This inhibition is caused by the binding of an AC-LL sequence present in the hinge segment to the ligand-binding site in the VHS domain. The inhibition depends on the phosphorylation of a serine located three residues upstream of the AC-LL motif. The serine is ...
SIGNOR-273623
Q13158
Q13233
0
phosphorylation
down-regulates activity
0.503
The results clearly show that fadd phosphorylation at ser194 affects functions both upstream and downstream of the mekk1/mkk7/jnk1 pathway and is closely associated with chemosensitivity in prostate cancer cells
SIGNOR-123168
P23528
Q96S53
0
phosphorylation
down-regulates activity
0.503
Like tesk1, tesk2 phosphorylated cofilin specifically at ser-3 and induced formation of actin stress fibers and focal adhesions.
SIGNOR-108753
P63000
P31431
0
binding
up-regulates activity
0.503
Rac1 is associated with Sdc4 and is activated by FN binding […] We observed that over-expression of Fzd7, or stimulation with FN resulted in increased levels of active Rac1 in primary myoblasts
SIGNOR-255849
Q92974
Q7KZI7
0
phosphorylation
down-regulates
0.503
We also show that par1b-induced serine 885/serine 959 phosphorylation inhibits rhoa-specific gef activity of gef-h1. As a consequence, gef-h1 phosphorylated on both of the serine residues loses the ability to stimulate rhoa and thereby fails to induce rhoa-dependent stress fiber formation
SIGNOR-177100
Q13546
Q9NWF9
0
ubiquitination
down-regulates quantity by destabilization
0.503
Triad3A promotes proteolytic degradation of adapter proteins. Triad3A promotes down-regulation of TIRAP, TRIF, and RIP1 proteins.
SIGNOR-271608
Q13873
Q9HAU4
0
ubiquitination
down-regulates
0.503
Smurf1 and smurf2 are e3 ubiquitin ligases known to suppress tgf-beta signaling through degra-dation of smads and receptors for tgf-beta and bmps.
SIGNOR-193119
Q9BRQ8
P04637
0
transcriptional regulation
up-regulates quantity by expression
0.503
The p53 tumor suppressor protein induces cell cycle arrest or apoptosis in response to cellular stresses. We have identified PRG3 (p53-responsive gene 3), which is induced specifically under p53-dependent apoptotic conditions in human colon cancer cells, and encodes a novel polypeptide of 373 amino acids with a predict...
SIGNOR-261808
P19086
P08913
0
binding
up-regulates activity
0.503
Here we systematically quantified ligand-induced interactions between 148 GPCRs and all 11 unique G alpha subunit C-termini. For each receptor, we probed chimeric G alpha subunit activation via a transforming growth factor-alpha (TGF alpha) shedding response in HEK293 cells lacking endogenous Gq/11- and G12/13- signali...
SIGNOR-257093
Q8IWQ3
Q15831
0
phosphorylation
up-regulates
0.503
Lkb1 is a master kinase that activates 13 kinases of the ampk subfamily, including mark/par-1we recently demonstrated that the lkb1 tumour suppressor kinase, in complex with the pseudokinase strad and the scaffolding protein mo25, phosphorylates and activates amp-activated protein kinase (ampk). A total of 12 human kin...
SIGNOR-122485