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PMID:2960 | Effect of variations in pH on kinetics of myosin. | The pH optimum was the same in canine tissue for cardiac and skeletal muscle myosin; when myosin was activated by monovalent cations, the pH optimum was 7.5 while activation of myosin by divalent cations gave a pH optimum of 5.5. Protons were needed for divalent cation activation of myosin. With changes in pH there wer... | Effect of variations in pH on kinetics of myosin. The pH optimum was the same in canine tissue for cardiac and skeletal muscle myosin; when myosin was activated by monovalent cations, the pH optimum was 7.5 while activation of myosin by divalent cations gave a pH optimum of 5.5. Protons were needed for divalent cation ... | [
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PMID:2961 | Distribution of prostaglandin E 9-ketoreductase and NAD+-dependent and NADP+-dependent 15-hydroxyprostaglandin dehydrogenase in the renal cortex and medulla of various species. | Regional distributions of PGE 9-ketoreductase and 15-hydroxy-prostaglandin dehydrogenase were examined in the cytoplasmic fractions from the kidneys of seven species. All species contained an NADPH-dependent reductase, as well as NAD+- and NADP+-dependent dehydrogenases in both cortex and medulla. A previously unrecogn... | Distribution of prostaglandin E 9-ketoreductase and NAD+-dependent and NADP+-dependent 15-hydroxyprostaglandin dehydrogenase in the renal cortex and medulla of various species. Regional distributions of PGE 9-ketoreductase and 15-hydroxy-prostaglandin dehydrogenase were examined in the cytoplasmic fractions from the ki... | [
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PMID:2962 | Normal blood-gas values in lambs during neonatal development and in adult sheep. | Arterial oxygen (PaO2) and carbon dioxide (PaCO2) tensions and pH were determined in 20 lambs during development from two weeks to five months of age and in 34 adult sheep. Throughout the period the lambs' mean PaCO2 (33.1 mm Hg) and pH (7.46) values were similar to those determined in the adult sheep (PaCO232.1 mm Hg;... | Normal blood-gas values in lambs during neonatal development and in adult sheep. Arterial oxygen (PaO2) and carbon dioxide (PaCO2) tensions and pH were determined in 20 lambs during development from two weeks to five months of age and in 34 adult sheep. Throughout the period the lambs' mean PaCO2 (33.1 mm Hg) and pH (7... | [
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PMID:2965 | [The clinical importance of angiography in the diagnosis of periarteritis nodosa]. | Report on a 53 years old patient with renal failure. Angiography showed multiple micro-aneurysms in kidneys and liver, in some instances also in the pancreatic, duodenal and mesenterial arteries. Muscle biopsy confirmed the suspicion of arteriitis nodosa. Clinically the diagnosis of necrotizing arteriitis is difficult.... | [The clinical importance of angiography in the diagnosis of periarteritis nodosa]. Report on a 53 years old patient with renal failure. Angiography showed multiple micro-aneurysms in kidneys and liver, in some instances also in the pancreatic, duodenal and mesenterial arteries. Muscle biopsy confirmed the suspicion of ... | [
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PMID:2967 | pH and the cytotoxicity of fluoride in an animal cell culture system. | To investigate the mechanism for the toxicity of silicate cement as observed in a cell culture system, the effects of pH and fluoride were tested on human epithelial cells (NCTC 2544). At pH 7.3, fluoride concentrations from 15 to 25 mug/ml (0.79 to 1.3 mM) had a growth inhibitory effect. When pH of the incubation medi... | pH and the cytotoxicity of fluoride in an animal cell culture system. To investigate the mechanism for the toxicity of silicate cement as observed in a cell culture system, the effects of pH and fluoride were tested on human epithelial cells (NCTC 2544). At pH 7.3, fluoride concentrations from 15 to 25 mug/ml (0.79 to ... | [
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PMID:2968 | Dysplasia of the testis and epididymis. | Various congenital malformations of the testis and the seminal pathways are illustrated by 10 cases. Though these malformations are often free from symptoms, an increasing number of them are now being discovered in connection with investigations of male infertility. In cases of azoospermia where testicular biopsies sho... | Dysplasia of the testis and epididymis. Various congenital malformations of the testis and the seminal pathways are illustrated by 10 cases. Though these malformations are often free from symptoms, an increasing number of them are now being discovered in connection with investigations of male infertility. In cases of a... | [
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PMID:2969 | [Lipoprotein X in hepatobiliary diseases]. | The diagnostic and prognostic reliability of lipoprotein-X (Lp-X) in demonstrating or ruling out cholestasis has been evaluated in a group of 80 patients with diseases of the liver and/or the biliary tracts, and in 103 subjects with various other diseases. The results of Lp-X detection were compared with the so-called ... | [Lipoprotein X in hepatobiliary diseases]. The diagnostic and prognostic reliability of lipoprotein-X (Lp-X) in demonstrating or ruling out cholestasis has been evaluated in a group of 80 patients with diseases of the liver and/or the biliary tracts, and in 103 subjects with various other diseases. The results of Lp-X ... | [
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PMID:2970 | [Proceedings: Home treatment of chronic respiratory insufficiency: inhalation of beta stimulators]. | In the author's experience, the longterm treatment of chronic obstructive lung disease should be supplemented by continuous selective stimulation of the adrenergic beta-receptors in the smooth bronchial muscles. The following procedure is recommended: inhalation of 15 min 3 times daily using an adequate aerosol with a ... | [Proceedings: Home treatment of chronic respiratory insufficiency: inhalation of beta stimulators]. In the author's experience, the longterm treatment of chronic obstructive lung disease should be supplemented by continuous selective stimulation of the adrenergic beta-receptors in the smooth bronchial muscles. The foll... | [
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PMID:2971 | [Proceedings: Long-term therapy with antibiotics in chronic bronchitis]. | Longterm therapy of chronic bacterial bronchitis assumes two forms: (a) therapy of acute exacerbations, and (b) continuous longterm prophylaxis, chiefly during the 4-7 winter months. Longterm prophylaxis should be confined exclusively to patients with two or more severe annual exacerbations. The commonest pathogens, Ha... | [Proceedings: Long-term therapy with antibiotics in chronic bronchitis]. Longterm therapy of chronic bacterial bronchitis assumes two forms: (a) therapy of acute exacerbations, and (b) continuous longterm prophylaxis, chiefly during the 4-7 winter months. Longterm prophylaxis should be confined exclusively to patients ... | [
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PMID:2972 | [Isolation, identification and quantitative determination of lysolecithin in the human gastric juice]. | Lysolecithin was isolated and identified from the gastric contents of 5 patients with gastric ulcer and erosive and atrophic gastritis. The methods used involved methanolic extration, column chromatography on silica gel and sephadex gel filtration, followed by preparative thin layer chromatography. Identification of ly... | [Isolation, identification and quantitative determination of lysolecithin in the human gastric juice]. Lysolecithin was isolated and identified from the gastric contents of 5 patients with gastric ulcer and erosive and atrophic gastritis. The methods used involved methanolic extration, column chromatography on silica g... | [
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PMID:2973 | [Prinzmetal angina: clinical aspects and coronarographic findings]. | The clinical course and coronary arteriographic findings in 5 patients with Prinzmetal's variant angina pectoris are reviewed. In 4 patients who had ST-elevations inferiorly, 1 had minimal, 1 only slight, and 1 medium coronary artery disease; 1 had coronary spasm. 1 patient with ST-elevation anteriorly had severe steno... | [Prinzmetal angina: clinical aspects and coronarographic findings]. The clinical course and coronary arteriographic findings in 5 patients with Prinzmetal's variant angina pectoris are reviewed. In 4 patients who had ST-elevations inferiorly, 1 had minimal, 1 only slight, and 1 medium coronary artery disease; 1 had cor... | [
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PMID:2974 | [Forms of so-called crytorchism. Studies on 579 patients]. | In a material of 579 patients the incidence of the different forms of cryptorchism is investigated. The findings reveal retractile testes in 16.4%, ectopic testes in 23.1%, inguinal retentions in 49.5%, abdominal retentions in 2.3% secondary cryptorchism in 3.5%, and monorchism and anorchism in 4.9%. These results are ... | [Forms of so-called crytorchism. Studies on 579 patients]. In a material of 579 patients the incidence of the different forms of cryptorchism is investigated. The findings reveal retractile testes in 16.4%, ectopic testes in 23.1%, inguinal retentions in 49.5%, abdominal retentions in 2.3% secondary cryptorchism in 3.5... | [
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PMID:2975 | [Sugar-free, tooth-protecting chewing gum and candy. Results of a 7-year study]. | Experiences of 7 years with sugarless chewing gums and lozenges (tab. I and II) regarding their tooth protective properties are reported. Telemetry of interproximal plaque pH allows to assess acid formation from carbohydrates by plaque bacteria under almost natural conditions. Altogether, 5 chewing gums and 8 lozenges ... | [Sugar-free, tooth-protecting chewing gum and candy. Results of a 7-year study]. Experiences of 7 years with sugarless chewing gums and lozenges (tab. I and II) regarding their tooth protective properties are reported. Telemetry of interproximal plaque pH allows to assess acid formation from carbohydrates by plaque bac... | [
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PMID:2978 | [Churg-Strauss syndrome. Its nosological place. Apropos of a new case diagnosed before death]. | Allergic granulomatous angeitis or Churg and Strauss disease, seems to form part of the disease of necrotising angeitis. Clinically, it may be considered when periarteritis nodosa is associated with asthma and marked eosinophilia. The diagnosis depends on the pathological findings including the venous and pulmonary les... | [Churg-Strauss syndrome. Its nosological place. Apropos of a new case diagnosed before death]. Allergic granulomatous angeitis or Churg and Strauss disease, seems to form part of the disease of necrotising angeitis. Clinically, it may be considered when periarteritis nodosa is associated with asthma and marked eosinoph... | [
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PMID:2979 | [Periarteritis nodosa and cancer. Apropos of 2 cases]. | The authors report two cases of periarteritis nodosa associated with carcinoma. The first case was a man with an anaplastic bronchial carcinoma, the second was a woman with a rectal adenocarcinoma. Study of the association of certain collagen diseases with carcinoma was made to emphasize the great rareness of this asso... | [Periarteritis nodosa and cancer. Apropos of 2 cases]. The authors report two cases of periarteritis nodosa associated with carcinoma. The first case was a man with an anaplastic bronchial carcinoma, the second was a woman with a rectal adenocarcinoma. Study of the association of certain collagen diseases with carcinom... | [
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PMID:2982 | Nonsurgical control of massive acute gastric mucosal hemorrhage with antacid neutralization of gastric content. | A simple and safe method of medical treatment for control of massive acute gastric mucosal hemorrhage is described. The procedure was developed from the observation that gastric HCl played a central role in peretuating the syndrome. The treatment consists in neutralization of the gastric acid with antacid to pH of 7.0.... | Nonsurgical control of massive acute gastric mucosal hemorrhage with antacid neutralization of gastric content. A simple and safe method of medical treatment for control of massive acute gastric mucosal hemorrhage is described. The procedure was developed from the observation that gastric HCl played a central role in p... | [
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PMID:2994 | Effect of total gastrectomy on acid proteolytic zymogens in serum and urine. | Proteolytic pH-activity curve and agar gel electrophoretic pattern of acid proteolytic zymogens in serum and urine were studied in totally gastrectomized patients. In serum, total gastrectomy was found to reduce proteolytic activity markedly, but no appreciable changes could be detected by electrophoretic analysis. In ... | Effect of total gastrectomy on acid proteolytic zymogens in serum and urine. Proteolytic pH-activity curve and agar gel electrophoretic pattern of acid proteolytic zymogens in serum and urine were studied in totally gastrectomized patients. In serum, total gastrectomy was found to reduce proteolytic activity markedly, ... | [
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PMID:2995 | [Pollen diet as an adjuvans of radiotherapy in gynecologic carcinomas]. | The introduction of a pollen diet as an adjuvant in the reduction of side effects during radiotherapy of patients with gynaecological cancer is described and its efficacy evaluated. 15 women with carcinoma of the cervix received a pollen diet during irradiation, whilst ten further patients receiving irradiation served ... | [Pollen diet as an adjuvans of radiotherapy in gynecologic carcinomas]. The introduction of a pollen diet as an adjuvant in the reduction of side effects during radiotherapy of patients with gynaecological cancer is described and its efficacy evaluated. 15 women with carcinoma of the cervix received a pollen diet durin... | [
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PMID:2996 | [Effects of lethal overexposure on gastric secretion in the pig]. | The characteristics of gastric secretion following acute gamma exposure (1500 rd) were studied in pigs with a small Pavlov stomach. Spontaneous secretion was practically inhibited when the animals were taken out of the irradiation cell. It slowly recovered in the following hours and its pH, first neutral, turned acid a... | [Effects of lethal overexposure on gastric secretion in the pig]. The characteristics of gastric secretion following acute gamma exposure (1500 rd) were studied in pigs with a small Pavlov stomach. Spontaneous secretion was practically inhibited when the animals were taken out of the irradiation cell. It slowly recover... | [
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PMID:2997 | A study of the host selection patterns of the mosquitoes of the Kisumu area of Kenya. | The results of 12,168 precipitin tests on blood meals of mosquitoes of the Kano Plain caught by a variety of catching techniques indicate that to gain an accurate overall picture of feeding patterns both the indoor and the outdoor biotope must be sampled. CDC light traps operated inside houses and Monkswood type light ... | A study of the host selection patterns of the mosquitoes of the Kisumu area of Kenya. The results of 12,168 precipitin tests on blood meals of mosquitoes of the Kano Plain caught by a variety of catching techniques indicate that to gain an accurate overall picture of feeding patterns both the indoor and the outdoor bio... | [
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PMID:3001 | The host component of the graft-versus-host reaction. A study on the popliteal lymph node reaction in the rat. | I have measured the proportion of host cells in a popliteal lymph node undergoing a graft-versus-host (GVH) reaction. By using cytotoxic alloantisera with specificity for donor and host Ag-B determined antigens, it was found that about 90% of live cells suspended from the node were of host origin. The role of host T ly... | The host component of the graft-versus-host reaction. A study on the popliteal lymph node reaction in the rat. I have measured the proportion of host cells in a popliteal lymph node undergoing a graft-versus-host (GVH) reaction. By using cytotoxic alloantisera with specificity for donor and host Ag-B determined antigen... | [
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PMID:3002 | Arboviruses isolated from mosquitos and man in Surinam. | In the capital of Surinam, and in parts of the coastal plain and the savannah belt 47 strains of arbovirus were isolated from mosquito pools and from sentinel mice. Most of the strains (51%) were of the Mucambo type. The other types were Guama, Bimiti, Una, Catu, Restan, Maru, and Kwatta. Culex portesi was found to be ... | Arboviruses isolated from mosquitos and man in Surinam. In the capital of Surinam, and in parts of the coastal plain and the savannah belt 47 strains of arbovirus were isolated from mosquito pools and from sentinel mice. Most of the strains (51%) were of the Mucambo type. The other types were Guama, Bimiti, Una, Catu, ... | [
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PMID:2999 | The use of pluronic polyols in the precipitation of plasma proteins and its application in the preparation of plasma derivatives. | Pluronic F-38 was used a precipitant of plasma proteins under varying conditions of pH and polymer concentration. Results indicated that marked differences in the solubility of the plasma proteins in F-38 solutions can be appled to the separation of plasma components. The feasibility of the industrial application of th... | The use of pluronic polyols in the precipitation of plasma proteins and its application in the preparation of plasma derivatives. Pluronic F-38 was used a precipitant of plasma proteins under varying conditions of pH and polymer concentration. Results indicated that marked differences in the solubility of the plasma pr... | [
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PMID:3000 | Preparation and storage of platelet concentrates. | A technique of platelet concentrate preparation and storage is presented which permits the maximum number of viable and functional platelets to be preserved for periods of 72 hours. Although the storage conditions must be followed precisely, the method is nevertheless simple to perform and does not require specialized ... | Preparation and storage of platelet concentrates. A technique of platelet concentrate preparation and storage is presented which permits the maximum number of viable and functional platelets to be preserved for periods of 72 hours. Although the storage conditions must be followed precisely, the method is nevertheless s... | [
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PMID:3003 | [Polysome ribonucleases of leukemic cells]. | The composition of ribosomal proteins isolated from normal homopoietic and leucemic cells was studied by analytic electrophoresis in polyacrylamide gel. It was found that acid-soluble proteins from polysomal complexes can be separated into 21-22 components in the acid system. There was no significant differences in pro... | [Polysome ribonucleases of leukemic cells]. The composition of ribosomal proteins isolated from normal homopoietic and leucemic cells was studied by analytic electrophoresis in polyacrylamide gel. It was found that acid-soluble proteins from polysomal complexes can be separated into 21-22 components in the acid system.... | [
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... |
PMID:3004 | [Comparative characteristics of catalase from the fungus Penicillium vitale, which is synthesized under different nutritional conditions]. | A comparative study of properties (absorption spectra, thermostability, pH optimum, polyacrylamide gel electrophoresis, DEAE-cellulose separation) and structure (amino acid composition, finger-prints, carbohydrate composition) was performed for P. vitale catalase synthesized under different medium conditions. In all ca... | [Comparative characteristics of catalase from the fungus Penicillium vitale, which is synthesized under different nutritional conditions]. A comparative study of properties (absorption spectra, thermostability, pH optimum, polyacrylamide gel electrophoresis, DEAE-cellulose separation) and structure (amino acid composit... | [
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PMID:3014 | Suprapubic paramedian laparotomy for equine abdominal cryptorchidism. | The history of techniques for abdominal equine cryptorchidectomy is briefly reviewed. The technique of suprapubic paramedian laparotomy as used since 1955 at the University of Liverpool veterinary field station is described in detail and an account given of the authors' experience with the technique in nearly 200 cases... | Suprapubic paramedian laparotomy for equine abdominal cryptorchidism. The history of techniques for abdominal equine cryptorchidectomy is briefly reviewed. The technique of suprapubic paramedian laparotomy as used since 1955 at the University of Liverpool veterinary field station is described in detail and an account g... | [
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PMID:3015 | [Bacteriological and physicochemical studies of the meat from poultry with acute Marek's disease]. | Studied were bacteriologically a total of 114 birds affected with acute Marek's disease (40 broilers, 44 growing layers, and 30 adult layers) as well as 100 healthy control birds of the same three categories. A chemical analysis was performed of the red and white meat of 20 of the diseased pullets and 20 of the disease... | [Bacteriological and physicochemical studies of the meat from poultry with acute Marek's disease]. Studied were bacteriologically a total of 114 birds affected with acute Marek's disease (40 broilers, 44 growing layers, and 30 adult layers) as well as 100 healthy control birds of the same three categories. A chemical a... | [
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PMID:3020 | [The function of shuttle systems of liver extramitochondrial hydrogen transport in experimental atherosclerosis]. | An enzymatic activity of shuttle systems for reduction equivalents transport (malate-aspartate, glycerophosphate, lactate, glutamate and beta-hydroxybutyrate dehydrogenase) was studied spectrophotometrically in liver tissue of intact rabbits and animals with experimental atherosclerosis. Content of malate, oxaloacetate... | [The function of shuttle systems of liver extramitochondrial hydrogen transport in experimental atherosclerosis]. An enzymatic activity of shuttle systems for reduction equivalents transport (malate-aspartate, glycerophosphate, lactate, glutamate and beta-hydroxybutyrate dehydrogenase) was studied spectrophotometricall... | [
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PMID:3022 | [The specificity of galactokinase induction in rat liver tissue under the effect of galactose]. | In liver tissue of rats, a conventional laboratory food of which was substituted for galactose-rich food, the galactokinase activity was increased, but the glucokinase was not affected. In the rats kept on a glucose-rich food the glucokinase (but not the galactokinase) was activated. Hydrocortisone injection induced an... | [The specificity of galactokinase induction in rat liver tissue under the effect of galactose]. In liver tissue of rats, a conventional laboratory food of which was substituted for galactose-rich food, the galactokinase activity was increased, but the glucokinase was not affected. In the rats kept on a glucose-rich foo... | [
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PMID:3019 | [Age-related properties of inductive synthesis of glucose-6-phosphatase, fructose-1,6-diphosphatase, tyrosine aminotransferase and tryptophan pyrrolase in hypothalamus stimulation]. | In aged rats electrostimulation of hypothalamus caused less distinct induction of the enzymes in liver tissue, kidney and spleen as compared with the adult animals. The activation of the total RNA synthesis (actinomycin D and olivomycin) eliminated the alterations in the enzymatic activities, caused by the stimulation ... | [Age-related properties of inductive synthesis of glucose-6-phosphatase, fructose-1,6-diphosphatase, tyrosine aminotransferase and tryptophan pyrrolase in hypothalamus stimulation]. In aged rats electrostimulation of hypothalamus caused less distinct induction of the enzymes in liver tissue, kidney and spleen as compar... | [
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PMID:3024 | [Determination of the transferase activity of L-asparaginase]. | A method for determination of the transferase activity of 1-asparaginase in presence of hydroxylamine is developed. The optimally determined quantity of the enzyme was from 0.7 to 20 i. u. The conditions optimal for the enzymatic reaction and for quantitative estimation of 1-aspartyl-beta-hydroxamic acid were studied. ... | [Determination of the transferase activity of L-asparaginase]. A method for determination of the transferase activity of 1-asparaginase in presence of hydroxylamine is developed. The optimally determined quantity of the enzyme was from 0.7 to 20 i. u. The conditions optimal for the enzymatic reaction and for quantitati... | [
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PMID:3021 | [The effect of lipids from typhoid endotoxin on the activity of some liver enzymes]. | Sublethal doses of typhoid endotoxin distinctly increased the activities of glutamate dehydrogenase and histidine ammonium lyase in liver tissue of mice within 3 hrs and the tyrosine transaminase activity within 6 hrs after a single intraperitoneal administration. Within 24 hrs normalzation of these enzyme activities a... | [The effect of lipids from typhoid endotoxin on the activity of some liver enzymes]. Sublethal doses of typhoid endotoxin distinctly increased the activities of glutamate dehydrogenase and histidine ammonium lyase in liver tissue of mice within 3 hrs and the tyrosine transaminase activity within 6 hrs after a single in... | [
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PMID:3027 | [Purification and properties of myeloperoxidase from mice leukocytes]. | Myeloperoxidase from leucocytes of peritoneal exudate was isolated by chromatography on DEAE-Sephadex, CM-cellulose and gel filtration on Sephadex G-75. The preparation obtained was homogenous as shown by repeated gel filtration and ultracentrifugation. The enzyme was purified 302-fold, with 16% yield. The sedimentatio... | [Purification and properties of myeloperoxidase from mice leukocytes]. Myeloperoxidase from leucocytes of peritoneal exudate was isolated by chromatography on DEAE-Sephadex, CM-cellulose and gel filtration on Sephadex G-75. The preparation obtained was homogenous as shown by repeated gel filtration and ultracentrifugat... | [
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PMID:3023 | [Changes in oxidative systems of liver microsomes in rats following a single administration of phenobarbital and morphine]. | During the first three hours after a single administration of phenobarbital or morphine into rats a marked increase was observed in the activity and content of the respiratory chain components responsible for the oxidation of NAD-H2 and NADP-H2 in liver microsomes. This activation of oxidative enzymes correlated with t... | [Changes in oxidative systems of liver microsomes in rats following a single administration of phenobarbital and morphine]. During the first three hours after a single administration of phenobarbital or morphine into rats a marked increase was observed in the activity and content of the respiratory chain components res... | [
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PMID:3025 | [Isolation and properties of 2 kallikreins from human saliva]. | Two kallikreins (K-I and K-II) were purified from mixed, parotid and submandibular human saliva. The kallikreins were separated by chromatography on CM-cellulose. The pH optima of activity were at pH 9.3 and pH 9.6-9.8; Km for BAEE was 1.10-3M and 4.10-3M, respectively. The esterase activity of K-I and K-II was inhibit... | [Isolation and properties of 2 kallikreins from human saliva]. Two kallikreins (K-I and K-II) were purified from mixed, parotid and submandibular human saliva. The kallikreins were separated by chromatography on CM-cellulose. The pH optima of activity were at pH 9.3 and pH 9.6-9.8; Km for BAEE was 1.10-3M and 4.10-3M, ... | [
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PMID:3026 | [The correlation between activity of enzymes participating in the formation and utilization of acetyl CoA in rabbit heart mitochondria in myocarditis and normal state]. | Activities in rabbit heart mitochondria of acetoacetyl-CoA-thyolase, pyruvate dehydrogenase, acetyl CoA-synthetase, citrate synthase and acetyl carnitine transferase were compared. These enzymes participate in formation and utilization of acetyl-CoA. The acetoacetyl-CoA-thyolase and acetyl CoA-synthetase were shown to ... | [The correlation between activity of enzymes participating in the formation and utilization of acetyl CoA in rabbit heart mitochondria in myocarditis and normal state]. Activities in rabbit heart mitochondria of acetoacetyl-CoA-thyolase, pyruvate dehydrogenase, acetyl CoA-synthetase, citrate synthase and acetyl carniti... | [
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PMID:3038 | [New values for the molar extinction coefficients of NADH and NADPH for the use in routine laboratories (author's transl)]. | Extensive re-investigations with regard to the molar extinction coefficients of NADH and NADPH proved that in future, calculations in routine work can be performed with the following much more accurate epsilon-values: 6.15 x 10(3) 1 x mol-1 x cm-1 at Hg 334 nm (NADH and NADPH), 6.3 X 10(3) 1 X mol-1 x cm-1 at 340 nm (N... | [New values for the molar extinction coefficients of NADH and NADPH for the use in routine laboratories (author's transl)]. Extensive re-investigations with regard to the molar extinction coefficients of NADH and NADPH proved that in future, calculations in routine work can be performed with the following much more acc... | [
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PMID:3039 | [The metabolism of delta4-3-oxosteroids in rat liver]. | The enzymatic reactions are described by which delta4-3-oxosteroids, specially testosterone, are inactivated in rat liver. The delta4-3-oxosteroid-5 alpha-reductase in liver microsomes was studied intensively and it was found that it is an enzyme system. The 5 alpha-reduction of testosterone with NADPH or with NADH dep... | [The metabolism of delta4-3-oxosteroids in rat liver]. The enzymatic reactions are described by which delta4-3-oxosteroids, specially testosterone, are inactivated in rat liver. The delta4-3-oxosteroid-5 alpha-reductase in liver microsomes was studied intensively and it was found that it is an enzyme system. The 5 alph... | [
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PMID:3040 | [An improved trihydroxyindole method for the determination of urinary catecholamines]. | Commercially available columns for ion exchange chromatography were used for the separation of catecholamines in urine. The estimation of catecholamines was performed fluorimetrically by a new trihydroxyindole method. The fluorescence of adrenaline or noradrenaline was enhanced, in comparison with other methods, by the... | [An improved trihydroxyindole method for the determination of urinary catecholamines]. Commercially available columns for ion exchange chromatography were used for the separation of catecholamines in urine. The estimation of catecholamines was performed fluorimetrically by a new trihydroxyindole method. The fluorescenc... | [
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PMID:3045 | [Biochemical investigations of cancer cachexia. II. Depletion of glycogenolysis and stimulation of gluconeogenesis in Walker carcinoma 256 bearing rats (author's transl)]. | 150-200 g heavy, Walker-carcinoma bearing, male Sprague-Dawley-rats showed rapid, tumour weight dependent, loss of liver glycogen until complete depletion in tumour groups heavier than 40 g/animal. Simultaneously the glycogen mobilization after massive glucagon stimulation, was successivly diminished and finally abolis... | [Biochemical investigations of cancer cachexia. II. Depletion of glycogenolysis and stimulation of gluconeogenesis in Walker carcinoma 256 bearing rats (author's transl)]. 150-200 g heavy, Walker-carcinoma bearing, male Sprague-Dawley-rats showed rapid, tumour weight dependent, loss of liver glycogen until complete dep... | [
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PMID:3046 | Oxidative decarboxylation of para-hydroxybenzoic acids by peroxidases under in vivo and in vitro conditions. | Oxidative decarbyxylation of p-hydroxybenzoic acids in plant cell suspension cultures is catalyzed by peroxidases. This reaction has been characterized in vivo and in vitro. Decarboxylation of substituted benzoic acids yields monomeric, dimeric and oligomeric benzoquinones. All peroxidases obtained from soybean (Glycin... | Oxidative decarboxylation of para-hydroxybenzoic acids by peroxidases under in vivo and in vitro conditions. Oxidative decarbyxylation of p-hydroxybenzoic acids in plant cell suspension cultures is catalyzed by peroxidases. This reaction has been characterized in vivo and in vitro. Decarboxylation of substituted benzoi... | [
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PMID:3047 | The regulation of glucose-6-phosphate dehydrogenase in chloroplasts. | Glucose-6-phosphate dehydrogenase from intact pea chloroplasts is partially membrane bound and inactivated upon illumination. The inhibitory effect of light can be abolished by addition of methylviologen. Kinetic experiments with glucose-6-phosphate dehydrogenase reveal that, in the dark, the enzyme activity is strongl... | The regulation of glucose-6-phosphate dehydrogenase in chloroplasts. Glucose-6-phosphate dehydrogenase from intact pea chloroplasts is partially membrane bound and inactivated upon illumination. The inhibitory effect of light can be abolished by addition of methylviologen. Kinetic experiments with glucose-6-phosphate d... | [
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PMID:3050 | Characterization of different deoxyribonucleases in human lymphocytes. | Four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. All activities hydrolyse DNA endonucleolytically. One neutral deoxyribonuclease found in the cytoplasmic fractio... | Characterization of different deoxyribonucleases in human lymphocytes. Four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. All activities hydrolyse DNA endonucleoly... | [
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PMID:3051 | Studies on fragments of rod outer segments from bovine retinas. | Monolayers of fragments of our rod segments were formed at an air-water interface. The area of these particles was measured as a function of the pH of the aqueous phase. A maximum area was measured at pH 6.5. The film was characterised by measuring the decrease in surface pressure, after compression, and the half time ... | Studies on fragments of rod outer segments from bovine retinas. Monolayers of fragments of our rod segments were formed at an air-water interface. The area of these particles was measured as a function of the pH of the aqueous phase. A maximum area was measured at pH 6.5. The film was characterised by measuring the dec... | [
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... |
PMID:3052 | The ionization and optical properties of oxoformycin. | Details are given of the ultraviolet, optical rotatory dispersion and fluorescence spectra of oxoformycin. The pK of formation of the monoanion is 8.6, at 25 degrees C. As formycin B has the same pK value, the ionization of both compounds is ascribed to dissociation of a proton from N-1. Both the neutral and monoanioni... | The ionization and optical properties of oxoformycin. Details are given of the ultraviolet, optical rotatory dispersion and fluorescence spectra of oxoformycin. The pK of formation of the monoanion is 8.6, at 25 degrees C. As formycin B has the same pK value, the ionization of both compounds is ascribed to dissociation... | [
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PMID:3048 | Antagonistic relationships between electron transport and P700 in chloroplasts and intact algae. | The effects of divalent salts and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) upon nicotinamide adenine dinucleotide phosphate (NADP) reduction and P700 in isolated chloroplasts are described and compared with the influence of DCMU on oxygen evolution and P700 in intact cells. Most experiments were carried out with ... | Antagonistic relationships between electron transport and P700 in chloroplasts and intact algae. The effects of divalent salts and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) upon nicotinamide adenine dinucleotide phosphate (NADP) reduction and P700 in isolated chloroplasts are described and compared with the influe... | [
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PMID:3049 | Energy-linked pyridine nucleotide transhydrogenase activity in photosynthetically grown Rhodopseudomonas palustris. | Rhodopseudomonas palustris (ATCC 17001) develops energy-dependent NADP+ transhydrogenase activity while growing photosynthetically on thiosulfate, formate, or acetate as the electron donors. The enzymatic activity is present in the supernatant fraction S-144 000. -- As reported, this fraction contains small membrane fr... | Energy-linked pyridine nucleotide transhydrogenase activity in photosynthetically grown Rhodopseudomonas palustris. Rhodopseudomonas palustris (ATCC 17001) develops energy-dependent NADP+ transhydrogenase activity while growing photosynthetically on thiosulfate, formate, or acetate as the electron donors. The enzymatic... | [
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PMID:3073 | [Findings of subpartal monitoring, status of the newborn infant and evaluation of cardiotocographic findings]. | The parameters of the intrapartal fetal CTG and the actual pH are related to each other and to the Apgar score of the newborn. The interpretation was carried out by means of groups of 300 to 600 cases. The actual fetal pH has the most compact relation to the Apgar score of the newborn (C equals 0,31). But also this con... | [Findings of subpartal monitoring, status of the newborn infant and evaluation of cardiotocographic findings]. The parameters of the intrapartal fetal CTG and the actual pH are related to each other and to the Apgar score of the newborn. The interpretation was carried out by means of groups of 300 to 600 cases. The act... | [
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PMID:3074 | [Influence of low molecular dextran on the acid-base balance in mother and fetus]. | 9 normal pregnant women in the active phase of labor were treated with 500 ml low molecular dextrane with a pH of 4,5 under standardised external conditions. Before and after this infusion simultaneous estimations of the maternal and fetal acid-base status were made. There was neither a maternal nor a fetal acidosis. | [Influence of low molecular dextran on the acid-base balance in mother and fetus]. 9 normal pregnant women in the active phase of labor were treated with 500 ml low molecular dextrane with a pH of 4,5 under standardised external conditions. Before and after this infusion simultaneous estimations of the maternal and fet... | [
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PMID:3077 | [Acetyl-CoA-synthetase activity of pigmented staphylococci]. | In 19 strains of staphylococci a study was made of the activity of acetyl-CoA-synthetase reaction. All the strains possessed an active enzymatic system transforming the acetate into an active form. The activity of acetyl-CoA-synthetase proved to be much greater in the pigmented staphyloccus strains than in the nonpigme... | [Acetyl-CoA-synthetase activity of pigmented staphylococci]. In 19 strains of staphylococci a study was made of the activity of acetyl-CoA-synthetase reaction. All the strains possessed an active enzymatic system transforming the acetate into an active form. The activity of acetyl-CoA-synthetase proved to be much great... | [
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PMID:3075 | [Acetylcholinesterase from the electric organ of the ray Torpedo marmorata]. | Studies have been made on substrate specificity of acetylcholinesterase (AChE;EC 3-1-1-7) from the electric organ of the ray T. marmorata with respect of choline and thiocholine esters, as well as on the effect of pH, salts and organophosphorus inhibitors (OPI) on the activity of the enzyme. Acetylcholine (ACh), propio... | [Acetylcholinesterase from the electric organ of the ray Torpedo marmorata]. Studies have been made on substrate specificity of acetylcholinesterase (AChE;EC 3-1-1-7) from the electric organ of the ray T. marmorata with respect of choline and thiocholine esters, as well as on the effect of pH, salts and organophosphoru... | [
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PMID:3078 | Isolation and properties of tRNA nucleotidyltransferase from wheat embryos. | From wheat embryos, tRNA nucleotidyltransferase (EC 2.7.7.25) was isolated. By chromatography on Sepharose 6B, DEAE-cellulose and affinity chromatography on tRNA-hydrazyl-Sepharose 4B, 7000-fold purification of the enzyme was achieved. The enzyme required for its activity Mg2+ or Mn2+ ion. ATP inhibited incorporation o... | Isolation and properties of tRNA nucleotidyltransferase from wheat embryos. From wheat embryos, tRNA nucleotidyltransferase (EC 2.7.7.25) was isolated. By chromatography on Sepharose 6B, DEAE-cellulose and affinity chromatography on tRNA-hydrazyl-Sepharose 4B, 7000-fold purification of the enzyme was achieved. The enzy... | [
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PMID:3076 | [Electronmicroscopic study of the gravity receptors in ctenophores]. | Secondarily sensitive receptor balancer cells comprise the gravity receptor in the aboral organ-polivalent organ of Ctenophora. Ctenophora are phylogenetically the first animals which created receptor cell supplied with a single flagellar kinocilium connected with the otolithic apparatus. The receptor balancer cells ar... | [Electronmicroscopic study of the gravity receptors in ctenophores]. Secondarily sensitive receptor balancer cells comprise the gravity receptor in the aboral organ-polivalent organ of Ctenophora. Ctenophora are phylogenetically the first animals which created receptor cell supplied with a single flagellar kinocilium c... | [
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PMID:3079 | Non-specific acetyl-CoA carboxylase and methylmalonyl-CoA carboxyltransferase in Streptomyces noursei var. polifungini. | 1. Acetyl-CoA carboxylase (EC 6.4.1.2) and methylmalonyl-CoA carboxyltransferase (EC 2.1.3.1) have been isolated from mycelia of Streptomyces noursei var. polifungini, and purified about 50-fold. 2. Both enzymes carboxylate acetyl-CoA and propionyl-CoA; the respective Km values are 1.1 and 1.6 mM with acetyl-CoA carbox... | Non-specific acetyl-CoA carboxylase and methylmalonyl-CoA carboxyltransferase in Streptomyces noursei var. polifungini. 1. Acetyl-CoA carboxylase (EC 6.4.1.2) and methylmalonyl-CoA carboxyltransferase (EC 2.1.3.1) have been isolated from mycelia of Streptomyces noursei var. polifungini, and purified about 50-fold. 2. B... | [
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PMID:3081 | Preoperative treatment of thyrotoxicosis with a beta-adrenergic blocking agent. | The clinical usefulness of propranolol as the sole drug in the preoperative treatment of thyrotoxicosis has been investigated. The effect of the drug on the serum levels of thyroxine and triiodothyronine has also been studied. Eight consecutive patients were treated. Four of these showed good clinical response to propr... | Preoperative treatment of thyrotoxicosis with a beta-adrenergic blocking agent. The clinical usefulness of propranolol as the sole drug in the preoperative treatment of thyrotoxicosis has been investigated. The effect of the drug on the serum levels of thyroxine and triiodothyronine has also been studied. Eight consecu... | [
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PMID:3082 | Reduction of 3-oxosteroids in human liver microsomes. | The 3alpha- and 3beta-reduction of the following steroids was studied in human liver microsomes: 5alpha-androstane-3,17-dione, 17beta-hydroxy-5alpha-androstan-3-one, 5alpha-pregnane-3,20-dione, 5beta-pregnane-3,20-dione, 3-oxo-5beta-cholanoic acid and 7alpha-hydroxy-5alpha-cholestan-3-one. With NADH as cofactor there w... | Reduction of 3-oxosteroids in human liver microsomes. The 3alpha- and 3beta-reduction of the following steroids was studied in human liver microsomes: 5alpha-androstane-3,17-dione, 17beta-hydroxy-5alpha-androstan-3-one, 5alpha-pregnane-3,20-dione, 5beta-pregnane-3,20-dione, 3-oxo-5beta-cholanoic acid and 7alpha-hydroxy... | [
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PMID:3083 | The fractional post-coital test performed in a square capillary tube. | A new method of examination of post-coital cervical mucus was developed, with special attention to the moving spermatozoa. The test is performed in a square capillary tube (edge 0,8 mm). The migration rate, -- velocity and -- direction are measured by means of an ocular micrometer, which is seen projected over the capi... | The fractional post-coital test performed in a square capillary tube. A new method of examination of post-coital cervical mucus was developed, with special attention to the moving spermatozoa. The test is performed in a square capillary tube (edge 0,8 mm). The migration rate, -- velocity and -- direction are measured b... | [
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PMID:3084 | I-cell disease (mucolipidosis II):a report on its pathology. | The single most characteristic morphological feature in I-cell disease (ICD) is the accumulation of membrane-bound vacuoles in mesenchymal cells (mainly fibroblasts). No true storage can be documented in those vacuoles. That their contents could have been dissolved during fixation or embedding remains however a possibi... | I-cell disease (mucolipidosis II):a report on its pathology. The single most characteristic morphological feature in I-cell disease (ICD) is the accumulation of membrane-bound vacuoles in mesenchymal cells (mainly fibroblasts). No true storage can be documented in those vacuoles. That their contents could have been dis... | [
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PMID:3085 | Histochemical and morphological changes in human muscle spindle in upper and lower motor neuron lesions. | The human intrafusal fibers were found to consist of two morphological and three histochemical (ATPase reaction) types. Two types of nuclear bag fibers were seen. Type A showed alkali stable and acid labile and Type B showed acid and alkali stable ATPase reaction. The nuclear chain fibers showed only alkali stable ATPa... | Histochemical and morphological changes in human muscle spindle in upper and lower motor neuron lesions. The human intrafusal fibers were found to consist of two morphological and three histochemical (ATPase reaction) types. Two types of nuclear bag fibers were seen. Type A showed alkali stable and acid labile and Type... | [
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PMID:3086 | [Effect of an anxiolytic agent in hay fever]. | In a group of 55 allergic patients with hay fever, and including patients treated by complete placebo, the action of Lorazepam has been studied from both the psycho-somatic and the allergic point of view. This most obviously allergic of all allergic diseases has been especially chosen because it was thought that the ps... | [Effect of an anxiolytic agent in hay fever]. In a group of 55 allergic patients with hay fever, and including patients treated by complete placebo, the action of Lorazepam has been studied from both the psycho-somatic and the allergic point of view. This most obviously allergic of all allergic diseases has been especi... | [
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PMID:3087 | A case of methylmalonic and propionic acidemia due to methulmalonyl-CoA carbonylmutase apoenzyme deficiency. | A patient presenting with a deep metabolic acidosis after birth is described. Gas chromatographic analysis of short chain fatty acid and non volatile organic acids revealed the presence of both propionic and methylmalonic acid. In plasma obtained immediately after death the propionic- and methylmalonic acid concentrati... | A case of methylmalonic and propionic acidemia due to methulmalonyl-CoA carbonylmutase apoenzyme deficiency. A patient presenting with a deep metabolic acidosis after birth is described. Gas chromatographic analysis of short chain fatty acid and non volatile organic acids revealed the presence of both propionic and met... | [
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PMID:3088 | Enzyme histochemical studies of rabbit bile ducts with and without bile flow. | The histochemical enzyme pattern in normal, extra-hepatic bile ducts from rabbits was studied. A difference between the duct epithelium and the crypt epithelium was noted mainly in the activity of gamma-glutamyl-transpeptidase and alkaline phosphatase which only stained positively in the crypts. No difference from the ... | Enzyme histochemical studies of rabbit bile ducts with and without bile flow. The histochemical enzyme pattern in normal, extra-hepatic bile ducts from rabbits was studied. A difference between the duct epithelium and the crypt epithelium was noted mainly in the activity of gamma-glutamyl-transpeptidase and alkaline ph... | [
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PMID:3089 | Survival of anaerobic bacteria during transportation. 1. Experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen. | The effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. Evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4 per cent. Three B. fragilis strains and one strain of Fusobacterium mortife... | Survival of anaerobic bacteria during transportation. 1. Experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen. The effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. Evacuation of atmospheri... | [
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PMID:3090 | The spread of the action potential through the T-system in hagfish twitch muscle fibres. | The input impedance of twitch muscle fibres of the Atlantic hagfish has been measured with sinusoidal transmembrane currents. The apparent specific membrane resistance and capacitance decreased markedly with frequency, and were relatively independent of fibre diameter. A model of the T-system based on anatomical observ... | The spread of the action potential through the T-system in hagfish twitch muscle fibres. The input impedance of twitch muscle fibres of the Atlantic hagfish has been measured with sinusoidal transmembrane currents. The apparent specific membrane resistance and capacitance decreased markedly with frequency, and were rel... | [
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PMID:3091 | Withdrawal psychosis: a study of 30 consecutive cases. | A study was carried out of 30 consecutive patients with withdrawal psychosis who in the period 1972 to 1975 were admitted to a psychiatric department or were attended by the department while hospitalized in a somatic department. There was a clear majority of women among cases of psychosis following drug withdrawal (15 ... | Withdrawal psychosis: a study of 30 consecutive cases. A study was carried out of 30 consecutive patients with withdrawal psychosis who in the period 1972 to 1975 were admitted to a psychiatric department or were attended by the department while hospitalized in a somatic department. There was a clear majority of women ... | [
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PMID:3092 | Nephroangiography in Wegener's granulumatosis. A comparison with panarteritis nodosa. | Three cases of Wegener's granulomatosis with a classical course are described, 2 of which with fatal outcome in spite of immuno-suppressive therapy. Nephroangiography was performed during the oliguric or anuric phase. The appearances were similar to those encountered in glomerulonephritis and were compared with those i... | Nephroangiography in Wegener's granulumatosis. A comparison with panarteritis nodosa. Three cases of Wegener's granulomatosis with a classical course are described, 2 of which with fatal outcome in spite of immuno-suppressive therapy. Nephroangiography was performed during the oliguric or anuric phase. The appearances ... | [
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PMID:3093 | The effect of metoprolol --a new selective adrenergic beta1-receptor blocking agent-- in mild hypertension. | Metoprolol, a new selective adrenergic beta1-receptor blocking drug, has been compared to placebo in a series of 24 women who previously had taken alprenolol and propranolol during a cross-over study. Blood pressure (BP) and heart rate (HR) were significantly reduced during metoprolol treatment compared to placebo. The... | The effect of metoprolol --a new selective adrenergic beta1-receptor blocking agent-- in mild hypertension. Metoprolol, a new selective adrenergic beta1-receptor blocking drug, has been compared to placebo in a series of 24 women who previously had taken alprenolol and propranolol during a cross-over study. Blood press... | [
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PMID:3094 | Comparison between metoprolol and propranolol as antihypertensive agents. A double-blind cross-over study. | A new selective beta-adrenergic blocking agent--metoprolol--is compared to a non-selective beta-adrenergic blocking agent--propranolol--according to a double-blind cross-over technique in 23 hypertensive women, who had previously taken alprenolol and propranolol during different periods. No significant differences were... | Comparison between metoprolol and propranolol as antihypertensive agents. A double-blind cross-over study. A new selective beta-adrenergic blocking agent--metoprolol--is compared to a non-selective beta-adrenergic blocking agent--propranolol--according to a double-blind cross-over technique in 23 hypertensive women, wh... | [
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PMID:3095 | Effects of metabolic and pharmacologic interventions on myocardial infarct size following coronary occlusion. | A number of hemodynamic, pharmacologic and metabolic interventions were found to change the extent of acute ischemic injury of the myocardium and subsequent necrosis following experimental coronary artery occlusion. Reduction in myocardial damage occurred by decreasing myocardial oxygen demands (beta-adrenergic blockin... | Effects of metabolic and pharmacologic interventions on myocardial infarct size following coronary occlusion. A number of hemodynamic, pharmacologic and metabolic interventions were found to change the extent of acute ischemic injury of the myocardium and subsequent necrosis following experimental coronary artery occlu... | [
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... |
PMID:3096 | Factors of importance for the degree of ischemic injury in the isolated rat heart. | Isolated working rat hearts were made ischemic by introducing a one-way aortic ball valve. After the ischemic period the hearts were perfused in a retrograde non-working way for 30 min. Flow rates, glycogen, ATP, and creatine-phosphate went down during the time of ischemia, whereas tissue lactate accumulated. For short... | Factors of importance for the degree of ischemic injury in the isolated rat heart. Isolated working rat hearts were made ischemic by introducing a one-way aortic ball valve. After the ischemic period the hearts were perfused in a retrograde non-working way for 30 min. Flow rates, glycogen, ATP, and creatine-phosphate w... | [
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... |
PMID:3098 | Double-blind study of the effect of cardioselective beta-blockade on chest pain in acute myocardial infarction. | A double-blind study including three different cardioselective beta-blockers, practolol, H 87/07 and metoprolol, was performed in 54 patients with acute myocardial infarction and chest pain shortly after onset of symptoms. Transmural infarctions were found in 42 patients while 12 patients had nontransmural infarctions.... | Double-blind study of the effect of cardioselective beta-blockade on chest pain in acute myocardial infarction. A double-blind study including three different cardioselective beta-blockers, practolol, H 87/07 and metoprolol, was performed in 54 patients with acute myocardial infarction and chest pain shortly after onse... | [
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PMID:3102 | Multiple endocrine adenomatosis syndromes. | MEA I and II are two genetically distinct tumor endocrinopathies, both showing autosomal dominant inheritance. Little overlap exists between these conditions, and that which is present can be explained on the basis of two mutually exclusive factors: (1) the secondary consequences of hormone excess on another endocrine ... | Multiple endocrine adenomatosis syndromes. MEA I and II are two genetically distinct tumor endocrinopathies, both showing autosomal dominant inheritance. Little overlap exists between these conditions, and that which is present can be explained on the basis of two mutually exclusive factors: (1) the secondary consequen... | [
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PMID:3108 | Folic acid absorption in regional enteritis. | The authors have investigated intestinal absorption of folic acid by jejunal perfusion with a triple lumen tube in five subjects with regional enteritis. The subjects' intestinal disabilities ranged from terminal ileitis to short bowel syndrome. Two had steatorrhea and two had low serum folate levels. Absorption of pte... | Folic acid absorption in regional enteritis. The authors have investigated intestinal absorption of folic acid by jejunal perfusion with a triple lumen tube in five subjects with regional enteritis. The subjects' intestinal disabilities ranged from terminal ileitis to short bowel syndrome. Two had steatorrhea and two h... | [
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PMID:3109 | Acid, neutral, and alkaline hydrolases in arthritic synovium. | The levels of six lysosomal enzymes (acid phosphatase, beta-acetylglucosaminidase, cathepsin D, beta-galactosidase, arylsulfatase A, and beta-glucuronidase) and four neutral and alkaline hydrolases (esterase, inorganic phyrophosphatase, alkaline phosphatase, and 5'-nucleotidase) were measured in osteoarthritic, rheumat... | Acid, neutral, and alkaline hydrolases in arthritic synovium. The levels of six lysosomal enzymes (acid phosphatase, beta-acetylglucosaminidase, cathepsin D, beta-galactosidase, arylsulfatase A, and beta-glucuronidase) and four neutral and alkaline hydrolases (esterase, inorganic phyrophosphatase, alkaline phosphatase,... | [
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PMID:3111 | Effects of acute metabolic acidosis on parathyroid hormone action and calcium mobilization. | Mechanisms through which metabolic acidosis increases calcium mobilization have been investigated in thyroparathyroidectomized rats with induction of acute metabolic acidosis by infusing NH4C1 intravenously. Acute metabolic acidosis directly raised serum calcium concentration and augmented the effect of parathyroid hor... | Effects of acute metabolic acidosis on parathyroid hormone action and calcium mobilization. Mechanisms through which metabolic acidosis increases calcium mobilization have been investigated in thyroparathyroidectomized rats with induction of acute metabolic acidosis by infusing NH4C1 intravenously. Acute metabolic acid... | [
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PMID:3112 | Metabolism of histamine in secreting and isolated canine stomach. | Total 14C activity in juice secreted by gastric pouches of six dogs and seven isolated canine stomachs was determined in response to intravenous and intra-arterial infusions of histamine and [14C]histamine. The proportions of 14C attributable to histamine, Nalpha-methylhistamine (NalphaMeH), Nalpha,Nalpha-dimethylhista... | Metabolism of histamine in secreting and isolated canine stomach. Total 14C activity in juice secreted by gastric pouches of six dogs and seven isolated canine stomachs was determined in response to intravenous and intra-arterial infusions of histamine and [14C]histamine. The proportions of 14C attributable to histamin... | [
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PMID:3113 | Intracellular bicarbonate of skeletal muscle under different metabolic states. | Intracellular bicarbonate of single muscle fibers in vivo was measured by a direct electrometric method simultaneously with the membrane PD in rats under seven different metabolic states. From the measured intracellular bicarbonate values and the PCO2, the bicarbonate equilibrium potential and the intracellular pH were... | Intracellular bicarbonate of skeletal muscle under different metabolic states. Intracellular bicarbonate of single muscle fibers in vivo was measured by a direct electrometric method simultaneously with the membrane PD in rats under seven different metabolic states. From the measured intracellular bicarbonate values an... | [
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PMID:3114 | Effect of acid lumen pH on potassium transport in renal cortical collecting tubules. | In order to determine the effect of acid lumen pH on renal tubular potassium transport, cortical collecting tubules were dissected from rabbit kidneys and perfused in vitro. When the pH of the perfusate was lowered from 7.4 to 6.8, potassium secretion into the tubule lumen decreased by an average of 47%. The transepith... | Effect of acid lumen pH on potassium transport in renal cortical collecting tubules. In order to determine the effect of acid lumen pH on renal tubular potassium transport, cortical collecting tubules were dissected from rabbit kidneys and perfused in vitro. When the pH of the perfusate was lowered from 7.4 to 6.8, pot... | [
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PMID:3115 | Reflex sympathetic tachycardia during intravenous infusions in chronic spinal cats. | The reflex tachycardia elicited by rapid intravenous infusions of a blood substitute was studied in 21 chronic cats with spinal sections at C8. All animals could breath spontaneously. The day after section the average resting heart rate (HR) and arterial pressure (AP) were 109 beats/min and 98/67 mmHg, respectively. Va... | Reflex sympathetic tachycardia during intravenous infusions in chronic spinal cats. The reflex tachycardia elicited by rapid intravenous infusions of a blood substitute was studied in 21 chronic cats with spinal sections at C8. All animals could breath spontaneously. The day after section the average resting heart rate... | [
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PMID:3116 | Sudden potential drop in bullfrog gastric mucosa. | The previously reported sudden potential drop (SPD), which occurs under anoxia in 10% CO2, has been further explored. We find several conditions necessary for this effect: 1) anoxia; 2) serosal pH less than 7.1;3) presence of chloride; and 4) a PD, either spontaneous or voltage clamped, which changes across the region ... | Sudden potential drop in bullfrog gastric mucosa. The previously reported sudden potential drop (SPD), which occurs under anoxia in 10% CO2, has been further explored. We find several conditions necessary for this effect: 1) anoxia; 2) serosal pH less than 7.1;3) presence of chloride; and 4) a PD, either spontaneous or... | [
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PMID:3117 | Cancer of the undescended or maldescended testis. | An analysis of 45 cryptorchids (by history or examination) with a testicular cancer treated at Memorial Hospital, between 1934 and 1973, is presented. Twenty-five patients had the cryptorchid state repaired at ages four to 27 years, either spontaneously or by orchiopexy or hormonal therapy. Ipsilateral (24) or contrala... | Cancer of the undescended or maldescended testis. An analysis of 45 cryptorchids (by history or examination) with a testicular cancer treated at Memorial Hospital, between 1934 and 1973, is presented. Twenty-five patients had the cryptorchid state repaired at ages four to 27 years, either spontaneously or by orchiopexy... | [
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PMID:3120 | Use of protease inhibitor (trasylol) and heparin in cardiorespiratory resuscitation. II. Gasometric investigations of arterial blood. | The purpose of the present work was to assess the effect of trasylol--an inhibitor of proteases--and heparin on gasometric values in arterial blood during cardio-respiratory resuscitation. The investigations were carried out on rabbits in three experimental groups. In group I the simplest resuscitation procedures were ... | Use of protease inhibitor (trasylol) and heparin in cardiorespiratory resuscitation. II. Gasometric investigations of arterial blood. The purpose of the present work was to assess the effect of trasylol--an inhibitor of proteases--and heparin on gasometric values in arterial blood during cardio-respiratory resuscitatio... | [
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PMID:3127 | Porcine malignant hyperthermia induced by halothane and succinylcholine: failure of treatment with procaine or procainamide. | Metabolic, hemodynamic and neuroendocrine responses to the combined use of halothane and succinylcholine (SCh) were measured in five normal swine and five swine susceptible to malignant hyperthermia (MH). Constant-volume ventilation was used, and no therapy was instituted. The overall response in susceptible swine was ... | Porcine malignant hyperthermia induced by halothane and succinylcholine: failure of treatment with procaine or procainamide. Metabolic, hemodynamic and neuroendocrine responses to the combined use of halothane and succinylcholine (SCh) were measured in five normal swine and five swine susceptible to malignant hyperther... | [
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PMID:3129 | [Comparative study of neuraminidases from "Diplococcus pneumoniae" and "Clostridium perfringens"]. | Neuraminidases have been purified from the culture medium of two microorganisms, one aerobic, Diplococcus neumoniae, the other anaerobic, Clostridium perfringens. The enzymatic properties of the 2 neuraminidases have been studied (pH optimum; effect of cations; activity toward different substrates: neuraminyllactose, d... | [Comparative study of neuraminidases from "Diplococcus pneumoniae" and "Clostridium perfringens"]. Neuraminidases have been purified from the culture medium of two microorganisms, one aerobic, Diplococcus neumoniae, the other anaerobic, Clostridium perfringens. The enzymatic properties of the 2 neuraminidases have been... | [
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PMID:3130 | [Carbohydrate containing media for the detection of urease in "Klebsiella"]. | Sone strains of Klebsiella pneumoniae and K. oxytoca grown on nutrient agar may appear "urease negative" in a Ferguson type reagent medium after a 24 h incubation at 37 degrees C. Amongst such 147 so called urease negative strains, urease has been detected within a few hours in 79 strains, when bacteria have grown on m... | [Carbohydrate containing media for the detection of urease in "Klebsiella"]. Sone strains of Klebsiella pneumoniae and K. oxytoca grown on nutrient agar may appear "urease negative" in a Ferguson type reagent medium after a 24 h incubation at 37 degrees C. Amongst such 147 so called urease negative strains, urease has ... | [
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PMID:3131 | [Ferrous ion oxidation and uranium solubilization from a lowgrade ore by "Thiobacillus ferrooxidans" (author's transl)]. | The microbiological oxidation of ferrous ion and the extraction of uranium from a low-grade ore has been studied using an adapted strain of Thiobacillus ferrooxidans. The effect of temperature, pH, volumetric oxygen transfer coefficient, K1a, and aeration number, Ia, on the activity of the microorganism has been determ... | [Ferrous ion oxidation and uranium solubilization from a lowgrade ore by "Thiobacillus ferrooxidans" (author's transl)]. The microbiological oxidation of ferrous ion and the extraction of uranium from a low-grade ore has been studied using an adapted strain of Thiobacillus ferrooxidans. The effect of temperature, pH, v... | [
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PMID:3133 | Ethanol and galactose metabolism as influenced by 4-methylpyrazole in alcoholics with and without nutritional deficiencies. Preliminary report of a new approach to pathogenesis and treatment in alcoholic liver disease. | 4-Methyl pyrazole (4-MP, a specific inhibitor of alcohol dehydrogenase) reduced ethanol elimination by 30-50% and completely removed the ethanol-induced inhibition of galactose elimination in 2 control subjects. Ethanol elimination was accelerated in 2 alcoholics with adequate nutrition, but the effect of 4-MP was comp... | Ethanol and galactose metabolism as influenced by 4-methylpyrazole in alcoholics with and without nutritional deficiencies. Preliminary report of a new approach to pathogenesis and treatment in alcoholic liver disease. 4-Methyl pyrazole (4-MP, a specific inhibitor of alcohol dehydrogenase) reduced ethanol elimination b... | [
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PMID:3144 | A method for the regulation of microbial population density during continuous culture at high growth rates. | A method for the continuous culture of microorganisms is described which employs growth-dependent pH changes to control the rate of addition of fresh medium to a culture vessel. The apparatus (the "phauxostat") supports, at constant pH, long-term continuous culture at rates near or at the maximum of which the organisms... | A method for the regulation of microbial population density during continuous culture at high growth rates. A method for the continuous culture of microorganisms is described which employs growth-dependent pH changes to control the rate of addition of fresh medium to a culture vessel. The apparatus (the "phauxostat") s... | [
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PMID:3145 | Pyruvate fermentation in Rhodospirillum rubrum and after transfer from aerobic to anaerobic conditions in the dark. | The fermentative metabolism of Rhodospirillum rubrum (strain Ha, F1, S1) was studied after transfering the cells from aerobic to anaerobic dark culture conditions. Pyruvate was metabolized mainly to acetate and formate, and to a lesser extent to CO2 and priopionate, by all strains. Therefore, pyruvate formate lyase wou... | Pyruvate fermentation in Rhodospirillum rubrum and after transfer from aerobic to anaerobic conditions in the dark. The fermentative metabolism of Rhodospirillum rubrum (strain Ha, F1, S1) was studied after transfering the cells from aerobic to anaerobic dark culture conditions. Pyruvate was metabolized mainly to aceta... | [
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PMID:3146 | The influence of foster-home care on psychiatric patients. | Adult patients placed into foster families by six administrations in three provinces of Canada were oberved at time of placement and followed up 18 months later. Over this period, they exhibited a substantial decline in symptoms-almost as great as with similar patients retained in the hospital. However, there was virtu... | The influence of foster-home care on psychiatric patients. Adult patients placed into foster families by six administrations in three provinces of Canada were oberved at time of placement and followed up 18 months later. Over this period, they exhibited a substantial decline in symptoms-almost as great as with similar ... | [
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PMID:3147 | Sialic acid in thyroglobulin. II. Influence of sialic acid on ionization of tyrosine residues. | During electrophoresis in polyacrylamide gel at pH 9-5, it was noticed that sialic acid-poor thyroglobulin contains fewer low molecular weight fragments than sialic acid-rich thyroglobulin. Splitting off of sialic acid diminishes thenumber of phenolic groups of tyrosine, MIT, DIT and T4, which dissociate below pH 12-5.... | Sialic acid in thyroglobulin. II. Influence of sialic acid on ionization of tyrosine residues. During electrophoresis in polyacrylamide gel at pH 9-5, it was noticed that sialic acid-poor thyroglobulin contains fewer low molecular weight fragments than sialic acid-rich thyroglobulin. Splitting off of sialic acid dimini... | [
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PMID:3148 | Prevention of experimental gastric ulcers in rats by dial derivatives. | In 111 rats divided into 5 groups, Ipronal (5-allyl-5-(beta-hydroxypropyl)-barbituric acid) protected rats from development of experimental ulceration of the gastric mucosa twice as effectively as diallylbarbituric acid and compound D2H (5-isopropyl-5-(beta-hydroxypropyl)-barbituric acid). Compound D1S (alpha-allophany... | Prevention of experimental gastric ulcers in rats by dial derivatives. In 111 rats divided into 5 groups, Ipronal (5-allyl-5-(beta-hydroxypropyl)-barbituric acid) protected rats from development of experimental ulceration of the gastric mucosa twice as effectively as diallylbarbituric acid and compound D2H (5-isopropyl... | [
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PMID:3150 | Kawasaki disease. Relationship with infantile periarteritis nodosa. | Until 1972, Kawasaki disease, or acute febrile infantile mucocutaneous lymph node syndrome (MCLS), was considered in Japan to be a nonfatal disease with a favorable prognosis. Based on the findings from two autopsy cases of MCLS, we believe that sudden and unexpected death during convalescence may be due to arterial le... | Kawasaki disease. Relationship with infantile periarteritis nodosa. Until 1972, Kawasaki disease, or acute febrile infantile mucocutaneous lymph node syndrome (MCLS), was considered in Japan to be a nonfatal disease with a favorable prognosis. Based on the findings from two autopsy cases of MCLS, we believe that sudden... | [
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PMID:3151 | Portal hypertension and gastric lesions in the rat. | Gastric lesions occurred spontaneously and were increased in number by seven hours of restraint stress in rats with portal vein constriction (PVC). Vagotomy and pyloroplasty protected the congested stomach from erosion formation with stress. Major weight loss occurred two days after PVC, but not thereafter. Platelet co... | Portal hypertension and gastric lesions in the rat. Gastric lesions occurred spontaneously and were increased in number by seven hours of restraint stress in rats with portal vein constriction (PVC). Vagotomy and pyloroplasty protected the congested stomach from erosion formation with stress. Major weight loss occurred... | [
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PMID:3154 | Scanning electron microscopy studies in muscular dystrophy. | Scanning electron microscopy of unmanipulated erythrocytes from patients with myotonic dystrophy or Duchenne dystrophy and patients who were Duchenne carriers showed a large increase in the number of stomatocytes over the number in normal controls. No specific morhologic changes that would differentiate any of the dyst... | Scanning electron microscopy studies in muscular dystrophy. Scanning electron microscopy of unmanipulated erythrocytes from patients with myotonic dystrophy or Duchenne dystrophy and patients who were Duchenne carriers showed a large increase in the number of stomatocytes over the number in normal controls. No specific... | [
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PMID:3153 | Comparison of the biological effects in rat of high doses of two 4-amino-7-chloroquinoline derivatives: chloroquine and glafenine. | When given orally in elevated but nonlethal doses (150 to 450 mg/kg, on 2 consecutive days), glafenine induces in rats (body weight 100 g) a transient nephritis with an increase in blood urea, hypertrophy of adrenals, and some changes in the serum proteinogram. These effects do not appear to be due to the 4-amino-7-chl... | Comparison of the biological effects in rat of high doses of two 4-amino-7-chloroquinoline derivatives: chloroquine and glafenine. When given orally in elevated but nonlethal doses (150 to 450 mg/kg, on 2 consecutive days), glafenine induces in rats (body weight 100 g) a transient nephritis with an increase in blood ur... | [
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PMID:3152 | A comparative study on the irreversible binding of labeled halothane trichlorofluoromethane, chloroform, and carbon tetrachloride to hepatic protein and lipids in vitro and in vivo. | 1) After intraperitoneal injection of labeled CCl4, CHCl3, and halothane in mice, 14C is preferentially bound to liver endoplasmic protein and lipid. A considerable activity is also associated with mitochondrial constituents. Maximal protein binding (nmol/mg): CCl4: 2.8 (0.5 hrs); CHCl3: 11.5 (6 hrs); halothane: 5 (6 h... | A comparative study on the irreversible binding of labeled halothane trichlorofluoromethane, chloroform, and carbon tetrachloride to hepatic protein and lipids in vitro and in vivo. 1) After intraperitoneal injection of labeled CCl4, CHCl3, and halothane in mice, 14C is preferentially bound to liver endoplasmic protein... | [
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PMID:3160 | Interaction of thermostable direct hemolysin of Vibrio parahaemolyticus with human erythrocytes. | The interaction between thermostable direct hemolysin produced by Vibrio parahaemolyticus WP-1 and human erythrocytes was studied. The lysis of human erythrocytes by the hemolysin was dependent of temperature and no hemolysis occurred at low temperature (0-4 C), but the hemolysin was adsorbed on human erythrocytes even... | Interaction of thermostable direct hemolysin of Vibrio parahaemolyticus with human erythrocytes. The interaction between thermostable direct hemolysin produced by Vibrio parahaemolyticus WP-1 and human erythrocytes was studied. The lysis of human erythrocytes by the hemolysin was dependent of temperature and no hemolys... | [
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PMID:3161 | Deoxyribonucleic acid polymerases of Euglena gracilis. Purification and properties of two distinct deoxyribonucleic acid polymerases of high molecular weight. | Two DNA polymerases of high molecular weight, pol A (mol.wt. 190 000) and pol B (mol.wt. 240 ooo), have been purified 6300-fold and 1600-fold respectively from an extramitochondrial supernatant of a bleached strain of Euglena gracilis. They have very similar requirements when assayed with an 'activated'-DNA primer-temp... | Deoxyribonucleic acid polymerases of Euglena gracilis. Purification and properties of two distinct deoxyribonucleic acid polymerases of high molecular weight. Two DNA polymerases of high molecular weight, pol A (mol.wt. 190 000) and pol B (mol.wt. 240 ooo), have been purified 6300-fold and 1600-fold respectively from a... | [
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0.04560075327754021,
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