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PMID:4088 | Rate enhancement specificity with alpha-chymotrypsin: temperature dependence of deacylation. | The relative rate of the hydrolysis of 2-(5-n-alkyl)furoyl-alpha-chymotrypsin reaches a maximum with the propyl derivative. The Arrhenius plots for the hydrolyses of the 2-furoyl-, 2-(5-ethyl)furoyl-, and 2-(5-n-propyl)furoyl-alpha-chymotrypsins display a discontinuity, while the plots obtained with the ramaining furoy... | Rate enhancement specificity with alpha-chymotrypsin: temperature dependence of deacylation. The relative rate of the hydrolysis of 2-(5-n-alkyl)furoyl-alpha-chymotrypsin reaches a maximum with the propyl derivative. The Arrhenius plots for the hydrolyses of the 2-furoyl-, 2-(5-ethyl)furoyl-, and 2-(5-n-propyl)furoyl-a... | [
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PMID:4089 | Optimal conditions and specificity of interaction of a distinct class of nonhistone chromosomal proteins with DNA. | A subclass of nonhistone chromatin proteins with high DNA affinity has been isolated from rat liver. The interaction of the isolated proteins with DNA in vitro was characterized utilizing a nitrocellulose filter binding technique. The temperature, time, concentration, ionic strength, and pH dependence were characterize... | Optimal conditions and specificity of interaction of a distinct class of nonhistone chromosomal proteins with DNA. A subclass of nonhistone chromatin proteins with high DNA affinity has been isolated from rat liver. The interaction of the isolated proteins with DNA in vitro was characterized utilizing a nitrocellulose ... | [
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PMID:4090 | The subcellular distribution of adenylate and guanylate cyclases in murine lymphoid cells. | Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate and... | The subcellular distribution of adenylate and guanylate cyclases in murine lymphoid cells. Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be dist... | [
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PMID:4091 | Fluorine-19 nuclear magnetic resonance study of fluorotyrosine alkaline phosphatase: the influence of zinc on protein structure and a conformational change induced by phosphate binding. | 19F nuclear magnetic resonance (NMR) spectroscopy has been used to study a fully active E. coli fluorotyrosine alkaline phosphatase. The fluorotyrosine resonances provide sensitive probes of the conformational states of the protein. They were used to follow the addition of zinc or cobalt to the apoprotein, and the titr... | Fluorine-19 nuclear magnetic resonance study of fluorotyrosine alkaline phosphatase: the influence of zinc on protein structure and a conformational change induced by phosphate binding. 19F nuclear magnetic resonance (NMR) spectroscopy has been used to study a fully active E. coli fluorotyrosine alkaline phosphatase. T... | [
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PMID:4092 | 31P nuclear magnetic resonance study of alkaline phosphatase: the role of inorganic phosphate in limiting the enzyme turnover rate at alkaline pH. | 31P nuclear magnetic resonance (NMR) was used to directly observe the binding of inorganic phosphate to alkaline phosphatase. Evidencq for the tight binding of 1.5-2.0 mol of inorganic phosphate per dimer of alkaline phosphatase is presented. Two distinct forms of bound phosphate are observed, one predominating above p... | 31P nuclear magnetic resonance study of alkaline phosphatase: the role of inorganic phosphate in limiting the enzyme turnover rate at alkaline pH. 31P nuclear magnetic resonance (NMR) was used to directly observe the binding of inorganic phosphate to alkaline phosphatase. Evidencq for the tight binding of 1.5-2.0 mol o... | [
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PMID:4093 | An analysis of the autophosphorylation of rabbit and human erythrocyte membranes. | The autophosphorylation of rabbit and human erythrocyte membranes has been studied under various experimental conditions. The phosphopeptides of the erythocyte membranes were identified using sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis followed by ratioautography. The pattern of phosphorylatiion of m... | An analysis of the autophosphorylation of rabbit and human erythrocyte membranes. The autophosphorylation of rabbit and human erythrocyte membranes has been studied under various experimental conditions. The phosphopeptides of the erythocyte membranes were identified using sodium dodecyl sulfate-polyacrylamide slab gel... | [
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PMID:4094 | Glutamine synthetase adenylyltransferase from Escherichia coli: purification and physical and chemical properties. | The glutamine synthetase adenylyltransferase (EC 2.7.7.42), WHIch catalyzes the adenylylation and deadenylylation of glutamine synthetase in E. coli, has been stabilized and purified 2200-fold to apparent homogeneity. Sedimentation and electrophoresis studies show that the native enzyme is a single polypeptide chain of... | Glutamine synthetase adenylyltransferase from Escherichia coli: purification and physical and chemical properties. The glutamine synthetase adenylyltransferase (EC 2.7.7.42), WHIch catalyzes the adenylylation and deadenylylation of glutamine synthetase in E. coli, has been stabilized and purified 2200-fold to apparent ... | [
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PMID:4095 | The esterification of dolichol by rat liver microsomes. | The incubation of 1-[3H)dolichols with cell-free preparations from various rat tissues resulted in the formation of a labeled material which possessed the characteristics of synthetic dolichol palmitate. Rat liver microsomes were found to be a good source of the acyltransferase activity, and the properties of the react... | The esterification of dolichol by rat liver microsomes. The incubation of 1-[3H)dolichols with cell-free preparations from various rat tissues resulted in the formation of a labeled material which possessed the characteristics of synthetic dolichol palmitate. Rat liver microsomes were found to be a good source of the a... | [
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PMID:4096 | Preparation of Fv fragment from the mouse myeloma XRPC-25 immunoglobulin possessing anti-dinitrophenyl activity. | The myeloma IgA protein produced by plasmacytoma XRPC-25, was isolated by affinity chromatography on dinitrophenyllysine-Sepharose. The affinity constant of the intact protein or its Fab' toward 2,4-dinitrophenyl-L-lysine (Dnp) was found to be 2.6 X 10(5) M-1. In order to prepare an Fv fragment (Hochman, J., Inbar, D.,... | Preparation of Fv fragment from the mouse myeloma XRPC-25 immunoglobulin possessing anti-dinitrophenyl activity. The myeloma IgA protein produced by plasmacytoma XRPC-25, was isolated by affinity chromatography on dinitrophenyllysine-Sepharose. The affinity constant of the intact protein or its Fab' toward 2,4-dinitrop... | [
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PMID:4097 | Purification and properties of a low molecular weight protein factor of mitochondrial energy-linked functions. | 1. A soluble protein with a molecular weight of 11-12-10(3) has been isolated from bovine-heart mitochondria, which stimulates the following ATP-dependent reactions of submitochondrial particles treated with 0.6 mM EDTA and 1 M NH4OH: reverse electron transfer from succinate to NAD, transhydrogenation from NADH to NADP... | Purification and properties of a low molecular weight protein factor of mitochondrial energy-linked functions. 1. A soluble protein with a molecular weight of 11-12-10(3) has been isolated from bovine-heart mitochondria, which stimulates the following ATP-dependent reactions of submitochondrial particles treated with 0... | [
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PMID:4098 | Effect of the transmembrane electric field on the photochemical and quenching properties of photosystem II in vivo. | The intermediate phase of fluorescence relaxation (lms-ls) (Joliot, P., Joliot, A., Bouges, B, and Barbieri, G. (1971) Photochem. Photobiol. 14, 287-305), following a single saturating flash, is shown to be controlled by a slow phase of the reoxidation of Q- by a secondary acceptor and, in vivo, by the transmembrane el... | Effect of the transmembrane electric field on the photochemical and quenching properties of photosystem II in vivo. The intermediate phase of fluorescence relaxation (lms-ls) (Joliot, P., Joliot, A., Bouges, B, and Barbieri, G. (1971) Photochem. Photobiol. 14, 287-305), following a single saturating flash, is shown to ... | [
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PMID:4099 | Determination of H+/e- ratios in chloroplasts with flashing light. | Using a rapid pH electrode, measurements were made of the flash-induced proton transport in isolated spinach chloroplasts. To calibrate the system, we assumed that in the presence of ferricyanide and in steady-state flashing light, each flash liberates from water one proton per reaction chain. We concluded that with bo... | Determination of H+/e- ratios in chloroplasts with flashing light. Using a rapid pH electrode, measurements were made of the flash-induced proton transport in isolated spinach chloroplasts. To calibrate the system, we assumed that in the presence of ferricyanide and in steady-state flashing light, each flash liberates ... | [
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PMID:4100 | [Study of the mechanism of the effect of extracellular pH on the synthesis of the oxidative complex (cytochrome a+a3) of Bacillus coagulans: relationship to the "glucose effect" and role of excreted coproporphyrin III (author's transl)]. | During the "respiratory adaptation" of Bacillus coagulans, it was possible to dissociate the kinetics of cytochrome a and a3 synthesis with carbon monoxide. The synthesis of cytochrome a3 is preferentially repressed when the pH of the incubation medium is pH 6.5 instead of pH 5.5. However, though the total synthesis of... | [Study of the mechanism of the effect of extracellular pH on the synthesis of the oxidative complex (cytochrome a+a3) of Bacillus coagulans: relationship to the "glucose effect" and role of excreted coproporphyrin III (author's transl)]. During the "respiratory adaptation" of Bacillus coagulans, it was possible to diss... | [
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PMID:4101 | The effect of retinol and retinoic acid on the testicular phospholipase a activity in retinol-deficient rats. | Both phospholipases A1 and A2 activities (EC 3.1.1.4) at pH 7.4 were found to be significantly decreased in retinol-deficient rat testes supplemented with retinoic acid as compared to retinol-fed controls using 1-acyl-2-[1-(14)C]-oleoyl-sn-glycero-3-phosphocholine as substrate. However, little or no difference was obse... | The effect of retinol and retinoic acid on the testicular phospholipase a activity in retinol-deficient rats. Both phospholipases A1 and A2 activities (EC 3.1.1.4) at pH 7.4 were found to be significantly decreased in retinol-deficient rat testes supplemented with retinoic acid as compared to retinol-fed controls using... | [
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PMID:4102 | Enzymatic properties of cloacin DF13 and kinetics of ribosome inactivation. | 1. The cloacin DF13-induced inactivation of ribosomes in vitro can be described as an enzyme-catalyzed reaction according to the Michaelis-Menten equation. Most probably the cloacin acts as a unique endoribonuclease. 2. At pH 7.8 and 37 degrees C the Km value for the reaction of cloacin DF13 with ribosomes is 13.2 - 10... | Enzymatic properties of cloacin DF13 and kinetics of ribosome inactivation. 1. The cloacin DF13-induced inactivation of ribosomes in vitro can be described as an enzyme-catalyzed reaction according to the Michaelis-Menten equation. Most probably the cloacin acts as a unique endoribonuclease. 2. At pH 7.8 and 37 degrees... | [
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PMID:4103 | Transfer RNA methyltransferase activity in paramecium aurelia. | The tRNA methyltransferases from Paramecium aurelia were investigated. The effects of varying the Mg2+ and NH4+ concentrations, pH, and temperature on the methylation of Escherichia coli B tRNA using extracts from P. aurelia were determined. Optimum tRNA methyltransferase activity was observed at pH 7.8 and 37 degrees ... | Transfer RNA methyltransferase activity in paramecium aurelia. The tRNA methyltransferases from Paramecium aurelia were investigated. The effects of varying the Mg2+ and NH4+ concentrations, pH, and temperature on the methylation of Escherichia coli B tRNA using extracts from P. aurelia were determined. Optimum tRNA me... | [
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PMID:4104 | The effect of silver ion binding and pH on the buoyant density of DNA and its use in fractionating heterogeneous DNA. | 1. The effect of pH on the buoyant density of the complexes of Ag+ with DNA has been studied using 3H-labeled human DNA and several bacterial DNAs to determine the conditions necessary for the maximum resolution of compositional heterogeneity. In neutral CS2SO4 density gradients, Ag+ complexes with (G - C)-rich compone... | The effect of silver ion binding and pH on the buoyant density of DNA and its use in fractionating heterogeneous DNA. 1. The effect of pH on the buoyant density of the complexes of Ag+ with DNA has been studied using 3H-labeled human DNA and several bacterial DNAs to determine the conditions necessary for the maximum r... | [
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PMID:4105 | Comparison of purple membrane from Halobacterium cutirubrum and Halobacterium halabium. | Direct comparison of purple membrane preparations from Halobacterium cutirubrum and Halobacterium halobium was carried out. Both preparations were found to be essentially identical with respect to their molecular weight, retinal content, lipid composition, fingerprinting of peptides from peptide digestion, electron mic... | Comparison of purple membrane from Halobacterium cutirubrum and Halobacterium halabium. Direct comparison of purple membrane preparations from Halobacterium cutirubrum and Halobacterium halobium was carried out. Both preparations were found to be essentially identical with respect to their molecular weight, retinal con... | [
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PMID:4106 | Kinetics of ion translocation across charged membranes mediated by a two-site transport mechanism. Effects of polyvalent cations upon rubidium uptake into yeast cells. | (1) The effect of surface charge upon the kinetics of monovalent cation translocation via a two-site mechanism is investigated theroretically. (2) According to the model dealt with, typical relations are expected for the dependence of the kinetic parameters of the translocation process upon the concentration of a polyv... | Kinetics of ion translocation across charged membranes mediated by a two-site transport mechanism. Effects of polyvalent cations upon rubidium uptake into yeast cells. (1) The effect of surface charge upon the kinetics of monovalent cation translocation via a two-site mechanism is investigated theroretically. (2) Accor... | [
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PMID:4107 | The structure of monellin and its relation to the sweetness of the protein. | The sweet protein monellin [1-3] has been shown to consist of two non-identical subunits of 50 and 42 amino acid residues, which were separated electrophoretically and chromatographically. Automatic sequential Edman degradation gave the complete sequence of the longer subunit, and a partial sequency of the shorter one.... | The structure of monellin and its relation to the sweetness of the protein. The sweet protein monellin [1-3] has been shown to consist of two non-identical subunits of 50 and 42 amino acid residues, which were separated electrophoretically and chromatographically. Automatic sequential Edman degradation gave the complet... | [
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PMID:4108 | Anomalous fluorescence of yeast 3-phosphoglucerate kinase. | The 3-phosphoglycerate kinase (EC 2.7.2.3) of yeast which contains two tryptophyl and eight tyrosyl residues per molecule, displayed an unusualy fluorescence emission spectrum with a maximum at 308 nm when excited at 280 nm. The emission peak shifted to 329 nm when excited at 295 nm. We could confirm that it was due to... | Anomalous fluorescence of yeast 3-phosphoglucerate kinase. The 3-phosphoglycerate kinase (EC 2.7.2.3) of yeast which contains two tryptophyl and eight tyrosyl residues per molecule, displayed an unusualy fluorescence emission spectrum with a maximum at 308 nm when excited at 280 nm. The emission peak shifted to 329 nm ... | [
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PMID:4109 | Binding of norgestrel to human plasma proteins. | Binding of [14, 15-3H](+/-)-norgestrel to human plasma proteins has been investigated. Norgestrel showed greater affinity to plasma than to human serum albumin indicating specific norgestrel binding protein(s) in the plasma. alpha1-acid glycoprotein showed high affinity for norgestrel when compared with human serum alb... | Binding of norgestrel to human plasma proteins. Binding of [14, 15-3H](+/-)-norgestrel to human plasma proteins has been investigated. Norgestrel showed greater affinity to plasma than to human serum albumin indicating specific norgestrel binding protein(s) in the plasma. alpha1-acid glycoprotein showed high affinity f... | [
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PMID:4110 | Equilbrium and kinetics of the unfolding of alpha-lactalbumin by guanidine hydrochloride (II). | The reversible unfolding of alpha-lactalbumin by guanidine hydrochloride, was studied at 25.0 degrees C in a relatively low concentration range of the denaturant (0.80-2.00 mol/l) by means of difference spectra and pH-jump measurements. The unfolding was shown to occur between two states, N and D, because apparent rate... | Equilbrium and kinetics of the unfolding of alpha-lactalbumin by guanidine hydrochloride (II). The reversible unfolding of alpha-lactalbumin by guanidine hydrochloride, was studied at 25.0 degrees C in a relatively low concentration range of the denaturant (0.80-2.00 mol/l) by means of difference spectra and pH-jump me... | [
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PMID:4111 | Fluorimetric studies of tryptophyl exposure in concanavalin A. | Studies of the iodide ion quenching of the intrinsic fluorescence of Concanavalin A indicate that 50% of the tryptophyl fluorescence originates from exposed residues. This agrees with the X-ray crystallographic determination that two of the four tryptophan residues in a Concanavalin A monomer are on the surface. Previo... | Fluorimetric studies of tryptophyl exposure in concanavalin A. Studies of the iodide ion quenching of the intrinsic fluorescence of Concanavalin A indicate that 50% of the tryptophyl fluorescence originates from exposed residues. This agrees with the X-ray crystallographic determination that two of the four tryptophan ... | [
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PMID:4112 | A study of Folch-Pi apoprotein. II. Relation between polymerization state and conformation. | A comparison of the conformation of Folch-Pi apoprotein in organic solvent and in aqueous solutions has been made by ESR, infrared and circular dichroism spectroscopy studies. Electrophoresis and ultracentrifugation have been carried out in order to correlate molecular weight and charge of the molecule with its conform... | A study of Folch-Pi apoprotein. II. Relation between polymerization state and conformation. A comparison of the conformation of Folch-Pi apoprotein in organic solvent and in aqueous solutions has been made by ESR, infrared and circular dichroism spectroscopy studies. Electrophoresis and ultracentrifugation have been ca... | [
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PMID:4113 | Response of the glycolysis of human erythrocytes to the transition from the oxygenated to the deoxygenated state at constant intracellular pH. | The time course of the rate of the glycolysis of human erythrocytes and of some metabolites were determined before and after rapid deoxygenation at constant intracellular pH. For this purpose stripped deoxygenated haemoglobin was used as a rapid oxygen acceptor. Deoxygenation causes an increase of the glycolytic rate b... | Response of the glycolysis of human erythrocytes to the transition from the oxygenated to the deoxygenated state at constant intracellular pH. The time course of the rate of the glycolysis of human erythrocytes and of some metabolites were determined before and after rapid deoxygenation at constant intracellular pH. Fo... | [
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PMID:4114 | Intracellular pH of frog sartorius muscle. | A weak base, morpholine, has been labelled with 3H and tested for its suitability as an indicator for intracellular pH, by distribution in the tissue water of frog sartorius muscle in the species Hyla litoria. Its pK'a at 20 degrees C in a solution of the same ionic strength as frog Ringer was found to be 8.45 +/- 0.02... | Intracellular pH of frog sartorius muscle. A weak base, morpholine, has been labelled with 3H and tested for its suitability as an indicator for intracellular pH, by distribution in the tissue water of frog sartorius muscle in the species Hyla litoria. Its pK'a at 20 degrees C in a solution of the same ionic strength a... | [
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PMID:4115 | Activation of murine lymphocytes by cyclic guanosine 3',5'-monophosphate: specificity and role in mitogen activity. | Cyclic guanosine 3',5'-monophosphate (cyclic GMP) stimulates nucleic acid synthesis in lymphocytes, and has been implicated as the intracellular effector of the actions of mitogenic agents on these cells. In the present study, we examined the specificity of the mitogenic activity of cyclic GMP and of its 8-bromo (Br) d... | Activation of murine lymphocytes by cyclic guanosine 3',5'-monophosphate: specificity and role in mitogen activity. Cyclic guanosine 3',5'-monophosphate (cyclic GMP) stimulates nucleic acid synthesis in lymphocytes, and has been implicated as the intracellular effector of the actions of mitogenic agents on these cells.... | [
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PMID:4116 | Characterization of protein kinases from bovine parotid glands. The effect of tolbutamide and its derivative on these partially purified enzymes. | 1. Four fractions of protein kinase (EC 2.7.1.37) activity (Peak IH, IIH, IIIC and IVC) have been resolved and partially purified from the 100 000 X g supernatant fraction of bovine parotid glands by DEAE-cellulose and phosphocellulose chromatographies. 2. The protein kinases of Peak IH and IIH were adenosine 3',5'-mon... | Characterization of protein kinases from bovine parotid glands. The effect of tolbutamide and its derivative on these partially purified enzymes. 1. Four fractions of protein kinase (EC 2.7.1.37) activity (Peak IH, IIH, IIIC and IVC) have been resolved and partially purified from the 100 000 X g supernatant fraction of... | [
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PMID:4117 | A study of the single polypeptide nature of rhodanese. A comparison of different preparations. | The enzyme rhodanese (EC 2.8.1.1) appears as a single polypeptide chain protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight of this species is approx. 33 000. This contrasts with previous reports that rhodanese behaves on gel filtration chromatography as a rapidly equilibrating mo... | A study of the single polypeptide nature of rhodanese. A comparison of different preparations. The enzyme rhodanese (EC 2.8.1.1) appears as a single polypeptide chain protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight of this species is approx. 33 000. This contrasts with previou... | [
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PMID:4118 | Characterization of intracellular esterase A from Bacillus subtilis. | Esterase A (EC 3.1.1.1) obtained by sonic disruption of Bacillus subtilis SR22 (spoA12, trpC2) was purified approximately 400-fold by differential chemical and heating precipitation, DEAE-cellulose chromatography, and Bio-Rad P-150 gel filtration chromatography, with an overall yield of 59%. The purified enzyme hydroly... | Characterization of intracellular esterase A from Bacillus subtilis. Esterase A (EC 3.1.1.1) obtained by sonic disruption of Bacillus subtilis SR22 (spoA12, trpC2) was purified approximately 400-fold by differential chemical and heating precipitation, DEAE-cellulose chromatography, and Bio-Rad P-150 gel filtration chro... | [
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PMID:4119 | [Cow's milk alkaline phospharase. II. Subunit structure, metalloproteic nature and kinetic parameters (author's transl)]. | Alkaline phosphatase (EC 3.1.3.1) from cow's milk as a dimer comprising two identical or very similar subunits of about 85 000 molecular weight. The enzyme contains 4.9 +/- 0.6 gatoms of zinc per mol of protein. The essential kinetic properties are the same as those of other alkaline phosphatases: variation of pH optim... | [Cow's milk alkaline phospharase. II. Subunit structure, metalloproteic nature and kinetic parameters (author's transl)]. Alkaline phosphatase (EC 3.1.3.1) from cow's milk as a dimer comprising two identical or very similar subunits of about 85 000 molecular weight. The enzyme contains 4.9 +/- 0.6 gatoms of zinc per mo... | [
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PMID:4120 | Kinetic properties of pulmonary angiotensin-converting enzyme. Hydrolysis of hippurylglycylglycine. | Some of the kinetic properties of angiotensin-converting enzyme (peptidyl-dipeptide hydrolase, EC 3.4.15.1) purified from hog lung have been determined using hippurylglycylglycine as substrate. The effects of pH and ionic environment on enzyme activity are complex and interdependent. At 0.1 M NaCl, the pH-activity curv... | Kinetic properties of pulmonary angiotensin-converting enzyme. Hydrolysis of hippurylglycylglycine. Some of the kinetic properties of angiotensin-converting enzyme (peptidyl-dipeptide hydrolase, EC 3.4.15.1) purified from hog lung have been determined using hippurylglycylglycine as substrate. The effects of pH and ioni... | [
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PMID:4121 | Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z. | Euglena gracilis z contained two succinic semialdehyde dehydrogenases (EC 1.2.1.16), one requiring NAD and the other NADP, and these isozymes were separated from each other and partially purified. The NAD-linked isozyme was relatively stable on storage at 5 degrees C whereas the NADP-linked one was extremely unstable u... | Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z. Euglena gracilis z contained two succinic semialdehyde dehydrogenases (EC 1.2.1.16), one requiring NAD and the other NADP, and these isozymes were separated from each other and partially pu... | [
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PMID:4122 | Activation of tyrosine hydroxylase by polyanions and salts. An electrostatic effect. | The activity of a partially purified preparation of tyrosine hydroxylase (EC 1.14.16.2) from the bovine caudate nucleus was increased by heparin, chondroitin sulfate, phosphatidylserine, polyacrylic acid, polyvinyl sulfuric acid and both poly-D-, and poly-L-glutamic acids, all polyanions. A variety of salts both activa... | Activation of tyrosine hydroxylase by polyanions and salts. An electrostatic effect. The activity of a partially purified preparation of tyrosine hydroxylase (EC 1.14.16.2) from the bovine caudate nucleus was increased by heparin, chondroitin sulfate, phosphatidylserine, polyacrylic acid, polyvinyl sulfuric acid and bo... | [
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PMID:4123 | Glycolipid glycosyl transferases of a hamster cell line in culture. I. Kinetic constants, substrate and donor nucleotide sugar specificities. | The properties of enzymes catalysing the transfer of a galactose from UDP-galactose to exogenous ceramide monohexoside and ceramide di-hexoside derived from the Syrian hamster cell line NIL 2 were studied. The products of these enzymes were characterized by chemical and enzymatic methods. Kinetic analyses showed that t... | Glycolipid glycosyl transferases of a hamster cell line in culture. I. Kinetic constants, substrate and donor nucleotide sugar specificities. The properties of enzymes catalysing the transfer of a galactose from UDP-galactose to exogenous ceramide monohexoside and ceramide di-hexoside derived from the Syrian hamster ce... | [
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PMID:4124 | Pigeon liver diacetyl reductase. Effects of pH on the kinetic parameters of the reaction. | (1) The pH dependence of the kinetic parameters of the reaction catalyzed by pigeon liver diacetyl reductase (EC 1.1.1.5) was investigated in the pH range 5.1-8.6. (2) From the results obtained it is postulated that: (a), a group of pK around 7, active in the protonated form, participates in the interaction of the enzy... | Pigeon liver diacetyl reductase. Effects of pH on the kinetic parameters of the reaction. (1) The pH dependence of the kinetic parameters of the reaction catalyzed by pigeon liver diacetyl reductase (EC 1.1.1.5) was investigated in the pH range 5.1-8.6. (2) From the results obtained it is postulated that: (a), a group ... | [
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PMID:4125 | Alkylation of cysteinyl residues of pig heart NAD-specific isocitrate dehydrogenase by iodoacetate. | Pig heart NAD-specific isocitrate dehydrogenase is inactivated by reaction with iodoacetate at pH 6.0. Loss of activity can be attributed to the formation of 1-2 mol of carboxymethyl-cysteine per peptide chain. The rate of inactivation is markedly decreased by the combined addition of Mn2+ and isocitrate, but not by al... | Alkylation of cysteinyl residues of pig heart NAD-specific isocitrate dehydrogenase by iodoacetate. Pig heart NAD-specific isocitrate dehydrogenase is inactivated by reaction with iodoacetate at pH 6.0. Loss of activity can be attributed to the formation of 1-2 mol of carboxymethyl-cysteine per peptide chain. The rate ... | [
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PMID:4126 | Purification and properties of NADP-dependent glutamate dehydrogenase from yeast nuclear fractions. | 1. NADP-dependent glutamate dehydrogenase (EC 1.4.1.4) extracted from nuclear fractions of Saccharomyces cerevisiae was partially purified. The final purification achieved was over 100-fold over the initial extract. 2. Cellulose acetate electrophoresis shows that the preparation is close to homogeneity and that the enz... | Purification and properties of NADP-dependent glutamate dehydrogenase from yeast nuclear fractions. 1. NADP-dependent glutamate dehydrogenase (EC 1.4.1.4) extracted from nuclear fractions of Saccharomyces cerevisiae was partially purified. The final purification achieved was over 100-fold over the initial extract. 2. C... | [
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PMID:4127 | Comparative kinetic studies on the L-type pyruvate kinase from rat liver and the enzyme phosphorylated by cyclic 3', 5'-AMP-stimulated protein kinase. | The kinetics of rat liver L-type pyruvate kinase (EC 2.7.1.40), phosphorylated with cyclic AMP-stimulated protein kinase from the same source, and the unphosphorylated enzyme have been compared. The effects of pH and various concentrations of substrates, Mg2+, K+ and modifiers were studied. In the absence of fructose 1... | Comparative kinetic studies on the L-type pyruvate kinase from rat liver and the enzyme phosphorylated by cyclic 3', 5'-AMP-stimulated protein kinase. The kinetics of rat liver L-type pyruvate kinase (EC 2.7.1.40), phosphorylated with cyclic AMP-stimulated protein kinase from the same source, and the unphosphorylated e... | [
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PMID:4128 | Denaturation-induced disulfide formation in the enzyme rhodanese. | The effect of denaturants on the quantitation of free sulfhydryl groups in the enzyme rhodanese (thiosulfate sulfurtransferase, EC 2.8.1.1) has been reinvestigated in some detail. The sulfhydryl assay with the colorimetric reagent 5, 5'-dithio-bis (2-nitrobenzoic acid) Nbs2 shows four sulfhydryl groups per enzyme molec... | Denaturation-induced disulfide formation in the enzyme rhodanese. The effect of denaturants on the quantitation of free sulfhydryl groups in the enzyme rhodanese (thiosulfate sulfurtransferase, EC 2.8.1.1) has been reinvestigated in some detail. The sulfhydryl assay with the colorimetric reagent 5, 5'-dithio-bis (2-nit... | [
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PMID:4129 | Studies on a 3beta-hydroxysteroid sulphotransferase from rat liver. | A steroid sulphotransferase (EC 2.8.2.2) was partially purified from female rat liver. The enzyme was active towards the substrates, dehydroepiandrosterone, epiandrosterone and pregnenolone but was inactive towards oestrogens, cholesterol and ergocalciferol. A pH optimum of 5.0 was recorded but the enzyme was unstable ... | Studies on a 3beta-hydroxysteroid sulphotransferase from rat liver. A steroid sulphotransferase (EC 2.8.2.2) was partially purified from female rat liver. The enzyme was active towards the substrates, dehydroepiandrosterone, epiandrosterone and pregnenolone but was inactive towards oestrogens, cholesterol and ergocalci... | [
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PMID:4130 | An ESR study of the influence of some physico-chemical factors on the conformation of a postsynaptic acetylcholinesterase. | 1. In a previous ESR study of a membrane acetylcholinesterase (EC 3.1.1.7) we found, contrary to observations by other authors, spectra indicating that the active serine might be located in a pocket of the enzyme surface. In order to inquire into this possibility, ESR spectra were studied under the influence of differe... | An ESR study of the influence of some physico-chemical factors on the conformation of a postsynaptic acetylcholinesterase. 1. In a previous ESR study of a membrane acetylcholinesterase (EC 3.1.1.7) we found, contrary to observations by other authors, spectra indicating that the active serine might be located in a pocke... | [
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PMID:4131 | Presence and androgen control of an alkaline phosphatase in the nucleus of rat ventral prostate. | The presence of alkaline phosphatase (EC 3.1.3.1) activity has been demonstrated in nuclei of rat ventral prostate. This enzyme activity remained after washing of isolated nuclei with 0.5% Triton X-100; an acid phosphatase initially present with the nuclear fraction was removed by this treatment. The nuclear alkaline p... | Presence and androgen control of an alkaline phosphatase in the nucleus of rat ventral prostate. The presence of alkaline phosphatase (EC 3.1.3.1) activity has been demonstrated in nuclei of rat ventral prostate. This enzyme activity remained after washing of isolated nuclei with 0.5% Triton X-100; an acid phosphatase ... | [
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PMID:4132 | Removal of phosphate groups from casein with potato acid phosphatase. | Potato acid phosphatase (EC 3.1.3.2) was used to remove the eight phosphate groups from alphas1-casein. Unlike most acid phosphatases, which are active at pH 6.0 or below, potato acid phosphatase can catalyze the dephosphorylation of alphas1-casein at pH 7.0. Although phosphate inhibition is considerable (K1=0.42 mM ph... | Removal of phosphate groups from casein with potato acid phosphatase. Potato acid phosphatase (EC 3.1.3.2) was used to remove the eight phosphate groups from alphas1-casein. Unlike most acid phosphatases, which are active at pH 6.0 or below, potato acid phosphatase can catalyze the dephosphorylation of alphas1-casein a... | [
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PMID:4133 | Multiple forms of cyclic nucleotide phosphodiesterase in pig epidermis. | Pig epidermal cyclic nucleotide phosphodiesterases (EC 3.1.4.16) have been partially purified by DEAE-cellulose column chromatography. At least three different forms of the epidermal phosphodiesterases were identified. They were cyclic GMP-specific, cyclic GMP- and cyclic AMP-hydrolyzing and apparently a cyclic AMP-spe... | Multiple forms of cyclic nucleotide phosphodiesterase in pig epidermis. Pig epidermal cyclic nucleotide phosphodiesterases (EC 3.1.4.16) have been partially purified by DEAE-cellulose column chromatography. At least three different forms of the epidermal phosphodiesterases were identified. They were cyclic GMP-specific... | [
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PMID:4134 | Ascorbic acid-2-sulfate sulfhohydrolase activity of human arylsulfatase A. | Pure human arylsulfatase A (EC 3.1.6.1) was found to hydrolyze ascorbic acid 2-sulfate to ascorbic acid and inorganic sulfate at rates from 200 to 2000 mumol/mg per h depending on the method of assay. This rate was lower than that observed with the synthetic substrate 4-nitrocatechol sulfate, but higher than that seen ... | Ascorbic acid-2-sulfate sulfhohydrolase activity of human arylsulfatase A. Pure human arylsulfatase A (EC 3.1.6.1) was found to hydrolyze ascorbic acid 2-sulfate to ascorbic acid and inorganic sulfate at rates from 200 to 2000 mumol/mg per h depending on the method of assay. This rate was lower than that observed with ... | [
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PMID:4135 | Human alpha-fucosidase. Single residual enzymatic form in fucosidosis. | Four major forms of alpha-fucosidase (EC 3.2.1.51) activity were separated by isoelectrofocusing from sera of normal control individuals. All forms shifted towards less acidic pI values after neuraminidase treatment. In two patients affected with fucosidosis, only a single major acidic peak was observed and this was af... | Human alpha-fucosidase. Single residual enzymatic form in fucosidosis. Four major forms of alpha-fucosidase (EC 3.2.1.51) activity were separated by isoelectrofocusing from sera of normal control individuals. All forms shifted towards less acidic pI values after neuraminidase treatment. In two patients affected with fu... | [
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PMID:4136 | Separation of two PZ-peptidases from bovine dental follicle. | Two PZ-peptidases (EC 3.4.-) (A and B) cleaving a synthetic substrate for collagenase, 4-phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg (PZ-peptide) have been separated from the particulate fraction of bovine dental follicle. PZ-peptidase A had a molecular weight of 220 000, an optimum pH at 8.0-8.5, and a Km v... | Separation of two PZ-peptidases from bovine dental follicle. Two PZ-peptidases (EC 3.4.-) (A and B) cleaving a synthetic substrate for collagenase, 4-phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg (PZ-peptide) have been separated from the particulate fraction of bovine dental follicle. PZ-peptidase A had a mole... | [
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PMID:4137 | Properties of the major carboxypeptidase in the larvae of the webbing clothes moth, Tineola bisselliella. | The larvae of the webbing clothes moth, Tineola bisselliella contain two carboxypeptidases (EC 3.4.12-) and one of these has been purified by preparative polyacrylamide gel electrophoresis. Its pH optimum for the hydrolysis of N-benzyloxycarbonyl-glycyl-leucine was pH 7.5-7.7 and its molecular weight as judged by gel f... | Properties of the major carboxypeptidase in the larvae of the webbing clothes moth, Tineola bisselliella. The larvae of the webbing clothes moth, Tineola bisselliella contain two carboxypeptidases (EC 3.4.12-) and one of these has been purified by preparative polyacrylamide gel electrophoresis. Its pH optimum for the h... | [
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PMID:4138 | Cathepsins B1 from human fetal membranes. | Cathepsins B1 (EC 3.4.22.1) were isolated from fetal membranes of human placenta, i.e. amnion and chorion-decidua. Purification of the enzymes was achieved by the freezing-thawing technique, ammonium sulphate fractionation and Sephadex gel filtration. Cathepsis B1 separated either from amnion or from chorion-decidua ex... | Cathepsins B1 from human fetal membranes. Cathepsins B1 (EC 3.4.22.1) were isolated from fetal membranes of human placenta, i.e. amnion and chorion-decidua. Purification of the enzymes was achieved by the freezing-thawing technique, ammonium sulphate fractionation and Sephadex gel filtration. Cathepsis B1 separated eit... | [
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PMID:4139 | Purification of multiple forms of adenosine deaminase from rabbit intestine. | Two forms of adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4), differing in molecular size, have been purified and obtained in homogeneous form from rabbit intestine. The purification procedures involved extraction with acetate buffer, pH 5.5, precipitation and fractional reextraction with (NH4)2SO4, ion-exch... | Purification of multiple forms of adenosine deaminase from rabbit intestine. Two forms of adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4), differing in molecular size, have been purified and obtained in homogeneous form from rabbit intestine. The purification procedures involved extraction with acetate buffe... | [
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PMID:4140 | Stimulation of photosystem I-induced oxidation of chloroplast cytochrome b-559 by pre-illumination and by low pH. | (1) The proportion of higher plant chloroplast cytochrome b-559 oxidizable during illumination by low intensity 732 nm light increases as the pH is decreased below 6.5. At pH 5.0-5.3 total oxidation is seen and subsequent red light can cause reduction of up to 2/3 of the oxidized cytochrome. The oxidation by far red li... | Stimulation of photosystem I-induced oxidation of chloroplast cytochrome b-559 by pre-illumination and by low pH. (1) The proportion of higher plant chloroplast cytochrome b-559 oxidizable during illumination by low intensity 732 nm light increases as the pH is decreased below 6.5. At pH 5.0-5.3 total oxidation is seen... | [
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PMID:4141 | The effect of formate on cytochrome aa3 and on electron transport in the intact respiratory chain. | 1. Formate inhibits cytochrome c oxidase activity both in intact mitochondria and submitochondrial particles, and in isolated cytochrome aa3. The inhibition increases with decreasing pH, indicating that HCOOH may be the inhibitory species. 2. Formate induces a blue shift in the absorption spectrum of oxidized cytochrom... | The effect of formate on cytochrome aa3 and on electron transport in the intact respiratory chain. 1. Formate inhibits cytochrome c oxidase activity both in intact mitochondria and submitochondrial particles, and in isolated cytochrome aa3. The inhibition increases with decreasing pH, indicating that HCOOH may be the i... | [
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PMID:4142 | The coupling factor of photophosphorylation and the electric properties of the thylakoid membrane. | The rate of ATP synthesis of illuminated chloroplasts is correlated with the electric conductance of their inner membranes. In agreement with previous studies it is shown that ATP synthesis is paralleled by an increased conductance of the thylakoid membrane. This conductance together with the ability to form ATP is abo... | The coupling factor of photophosphorylation and the electric properties of the thylakoid membrane. The rate of ATP synthesis of illuminated chloroplasts is correlated with the electric conductance of their inner membranes. In agreement with previous studies it is shown that ATP synthesis is paralleled by an increased c... | [
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PMID:4143 | Evidence against proton gradient formation being the cause of chlorophyll fluorescence quenching by N-methylphenazonium methosulfate. | In strong illumination, 3-(3, 4-dichlorophenyl)-1,1-dimethylurea (DCMU)-poisoned chloroplasts exhibit a high yield of chlorophyll fluorescence while P-700 turnover, proton uptake, and phosphorylation are inhibited and a pH gradient is undectectable. When 10muM N-methylphenazonium methosulfate (PMS) is included, the flu... | Evidence against proton gradient formation being the cause of chlorophyll fluorescence quenching by N-methylphenazonium methosulfate. In strong illumination, 3-(3, 4-dichlorophenyl)-1,1-dimethylurea (DCMU)-poisoned chloroplasts exhibit a high yield of chlorophyll fluorescence while P-700 turnover, proton uptake, and ph... | [
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PMID:4144 | The accumulation of superoxide radical during the aerobic action of xanthine oxidase. A requiem for H2O4. | The action of xanthine oxidase upon acetaldehyde or xanthine at pH 10.2 has been shown to be accompanied by substantial accumulation of O2- during the first few minutes of the reaction. H2O2 decreases this accumulation of O2- presumably because of the Haber-Weiss reaction (H2O2+O2- leads to OH- +OH+O2) and very small a... | The accumulation of superoxide radical during the aerobic action of xanthine oxidase. A requiem for H2O4. The action of xanthine oxidase upon acetaldehyde or xanthine at pH 10.2 has been shown to be accompanied by substantial accumulation of O2- during the first few minutes of the reaction. H2O2 decreases this accumula... | [
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PMID:4145 | The 520 nm absorbance changes in Scenedesmus obliquus and its relation to photosystem I. | The kinetics (region of seconds) of the light-induced 520 nm absorbance changes and its dark reversal have been studied in detail in the wild type and in some pigment and photosynthetic mutants of Scenedesmus obliquus. The following 5 lines of evidence led us to conclude that the signal is entirely due to the photosyst... | The 520 nm absorbance changes in Scenedesmus obliquus and its relation to photosystem I. The kinetics (region of seconds) of the light-induced 520 nm absorbance changes and its dark reversal have been studied in detail in the wild type and in some pigment and photosynthetic mutants of Scenedesmus obliquus. The followin... | [
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PMID:4147 | Pyruvate flux into resealed ghosts from human erythrocytes. | The kinetics of pyruvate transport across the isolated red blood cell membrane were studied by a simple and precise spectrophotometric method: following the oxidation of NADH via lactate dehydrogenase trapped within resealed ghosts. The initial rate of pyruvate entry was linear. Influx was limited by saturation at high... | Pyruvate flux into resealed ghosts from human erythrocytes. The kinetics of pyruvate transport across the isolated red blood cell membrane were studied by a simple and precise spectrophotometric method: following the oxidation of NADH via lactate dehydrogenase trapped within resealed ghosts. The initial rate of pyruvat... | [
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PMID:4148 | Heme models. I. Solution behavior of a water soluble iron porphyrin. | A well-behaved water soluble iron-porphyrin system, meso-tetra-(4-carboxyphenyl) porphinato iron (III) was synthesized. Its solution behavior is described using visable and electron paramagnetic resonance (EPR) spectroscopy. The complex exists in solution as three distinct forms of bridged dimers, oxo, hydroxo and aquo... | Heme models. I. Solution behavior of a water soluble iron porphyrin. A well-behaved water soluble iron-porphyrin system, meso-tetra-(4-carboxyphenyl) porphinato iron (III) was synthesized. Its solution behavior is described using visable and electron paramagnetic resonance (EPR) spectroscopy. The complex exists in solu... | [
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PMID:4149 | 17 beta-Hydroxysteroid dehydrogenase of the sheep ovary : purification, properties and substrate binding site. | Sheep ovarian 17 beta HSDH has been purified about 1000 fold to a specific activity of 0.5 IU/mg protein, using DEAE cellulose chromatography, affinity chromatography on estrone-amino caproate-Sepharose and a second DEAE cellulose chromatography. The molecular weight is 70,000 ; the pH optimum for activity is 9.2 and t... | 17 beta-Hydroxysteroid dehydrogenase of the sheep ovary : purification, properties and substrate binding site. Sheep ovarian 17 beta HSDH has been purified about 1000 fold to a specific activity of 0.5 IU/mg protein, using DEAE cellulose chromatography, affinity chromatography on estrone-amino caproate-Sepharose and a ... | [
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PMID:4150 | Complete purification and studies on the structural and kinetic properties of two forms of yeast valyl-tRNA synthetase. | Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species mainl... | Complete purification and studies on the structural and kinetic properties of two forms of yeast valyl-tRNA synthetase. Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other ... | [
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PMID:4151 | [Rat liver plasma membrane phospholipase A]. | The plasma membranes phospholipase A2 studied in situ shows very little sensitivity for pH variations ; the optimal concentration for Ca++ is 5 mM ; the enzymatic kinetics are of the Michaelis type. An inactive state of the phospholipase A2 exists : when rats are injected with heparin, their plasma membranes contain a ... | [Rat liver plasma membrane phospholipase A]. The plasma membranes phospholipase A2 studied in situ shows very little sensitivity for pH variations ; the optimal concentration for Ca++ is 5 mM ; the enzymatic kinetics are of the Michaelis type. An inactive state of the phospholipase A2 exists : when rats are injected wi... | [
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PMID:4152 | Phosphate uptake in Chlorella pyrenoidosa : II. Effect of pH and of SH reagents. | The sensitivity of the phosphate transport system to pCMPS after phosphate starvation is dependent on protein synthesis. This fact is related to the development of transport activity at alkaline pH. In non-starved cells, the presence of only one peak of maximal activity for phosphate uptake at neutral pH (at low and hi... | Phosphate uptake in Chlorella pyrenoidosa : II. Effect of pH and of SH reagents. The sensitivity of the phosphate transport system to pCMPS after phosphate starvation is dependent on protein synthesis. This fact is related to the development of transport activity at alkaline pH. In non-starved cells, the presence of on... | [
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0.03964883089065552,
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... |
PMID:4156 | Possible occurrence for histidyl and cysteyl residues in the catalytic center of rat liver mitochondrial D (-)-beta-hydroxybutyrate dehydrogenase. | 1. Rat liver mitochondrial D(-)-beta-hydroxybutyrate dehydrogenase (submitochondrial particles and partially purified preparation) is inhibited by some dicarboxylates, especially by malonate and succinate. The inhibition is reversible and competitive with beta-hydroxybutyrate while uncompetitive with acetoacetate, NAD ... | Possible occurrence for histidyl and cysteyl residues in the catalytic center of rat liver mitochondrial D (-)-beta-hydroxybutyrate dehydrogenase. 1. Rat liver mitochondrial D(-)-beta-hydroxybutyrate dehydrogenase (submitochondrial particles and partially purified preparation) is inhibited by some dicarboxylates, espec... | [
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PMID:4153 | [Mechanism of glutaraldehyde-protein bond formation]. | Commercial aqueous 25% glutaraldehyde solutions contain no stable derivative of this aldehyde, but compounds of variable molecular weight which easily revert to glutaraldehyde. The effect of pH on the reaction of glutarldehyde with amino acids and on the stability of the products under acid conditions, shows the import... | [Mechanism of glutaraldehyde-protein bond formation]. Commercial aqueous 25% glutaraldehyde solutions contain no stable derivative of this aldehyde, but compounds of variable molecular weight which easily revert to glutaraldehyde. The effect of pH on the reaction of glutarldehyde with amino acids and on the stability o... | [
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0.02... |
PMID:4157 | Purification and properties of cyclic AMP dependent and independent protein kinases from rat pancreas. | Three protein kinases Ko, K1, and KII have been extracted from rat pancreas homogenate, Ko is not stimulated by cyclic AMP. K1 is poorly stimulated by cyclic AMP (1.3 times), Ku is highly stimulated (6 times). The specificity of KII with respect to various nucleotides and cyclic nucleotides has been determined. K1 and ... | Purification and properties of cyclic AMP dependent and independent protein kinases from rat pancreas. Three protein kinases Ko, K1, and KII have been extracted from rat pancreas homogenate, Ko is not stimulated by cyclic AMP. K1 is poorly stimulated by cyclic AMP (1.3 times), Ku is highly stimulated (6 times). The spe... | [
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0.014513247646391392,
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... |
PMID:4158 | [Decanoic acid, new precursor for in vitro biosynthesis of oleic acid by a plant subcellular fraction]. | Various membraneous fractions prepared from a cauliflower homogenate synthesize radioactive oleic acid when they are incubated in a 14C-decanolate solution. The more active fraction is formed of vesicles sedimenting at 30,000 g x 20 mn (heavy microsomes). The labelled precursor is transformed by this fraction mainly in... | [Decanoic acid, new precursor for in vitro biosynthesis of oleic acid by a plant subcellular fraction]. Various membraneous fractions prepared from a cauliflower homogenate synthesize radioactive oleic acid when they are incubated in a 14C-decanolate solution. The more active fraction is formed of vesicles sedimenting ... | [
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PMID:4154 | [Acetylcholinesterase. II. Experimental aspects of interaction with reversible effectors under conditions of high ionic strength]. | Interaction of usual effectors with acetylcholinesterase (EC 3.1.1.7) from bovine erythrocytes was examined under conditions of high ionic strength (gamma/2 greater than or equal to 0,1). Detailed kinetic investigation of the hydrolysis of acetylcholine by acetylcholinesterase in the presence of modifiers shows that th... | [Acetylcholinesterase. II. Experimental aspects of interaction with reversible effectors under conditions of high ionic strength]. Interaction of usual effectors with acetylcholinesterase (EC 3.1.1.7) from bovine erythrocytes was examined under conditions of high ionic strength (gamma/2 greater than or equal to 0,1). D... | [
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PMID:4159 | [Interactions of phosphorylethanolamine analogs with phosphorylethanolamine-citidylyltransferase]. | Kinetic studies of ethanolaminephosphate-cytidylyltransferase (E.C. 2.7.7.14) from rat liver have been carried out in presence of structural analogues of ethanolaminephosphate : these compounds acted as inhibitors of the enzyme: - 2-aminoethylphosphonate behaved as a substrate and a competitive inhibitor to phosphoryle... | [Interactions of phosphorylethanolamine analogs with phosphorylethanolamine-citidylyltransferase]. Kinetic studies of ethanolaminephosphate-cytidylyltransferase (E.C. 2.7.7.14) from rat liver have been carried out in presence of structural analogues of ethanolaminephosphate : these compounds acted as inhibitors of the ... | [
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PMID:4160 | [Purification and properties of aromatic L-amino acid decarboxylase (4.1.1.28) of rat brain]. | L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the ins... | [Purification and properties of aromatic L-amino acid decarboxylase (4.1.1.28) of rat brain]. L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have ... | [
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PMID:4161 | [Characterization of some hydrolase activities in digestive juice of Achatina balteata]. | The digestive juice of Achatina balteata, a giant snail of the West African Coast catalyses the hydrolysis of several natural and synthetic compounds. Enzymatic activities on lactose, o- and p-nitrophenyl-beta-D-galactoside, p-nitrophenyl-beta-D-glucoside, p-nitrophenyl-beta-D (and alpha-L-) fucoside, o-nitrophenyl-bet... | [Characterization of some hydrolase activities in digestive juice of Achatina balteata]. The digestive juice of Achatina balteata, a giant snail of the West African Coast catalyses the hydrolysis of several natural and synthetic compounds. Enzymatic activities on lactose, o- and p-nitrophenyl-beta-D-galactoside, p-nitr... | [
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PMID:4162 | Characterization of the mannosyl and fucosyl transferases in the ovine anterior pituitary glands. | Ovine anterior pituitary glands contain mannosyl- and fucosyl-transferases localized in the microsomes and able to incorporate mannose or fucose as such from GDP-mannose or GDP-fucose into endogenous glycoproteins. The requirements and conditions necessary for maximum activity were investigated. The value of the Km is ... | Characterization of the mannosyl and fucosyl transferases in the ovine anterior pituitary glands. Ovine anterior pituitary glands contain mannosyl- and fucosyl-transferases localized in the microsomes and able to incorporate mannose or fucose as such from GDP-mannose or GDP-fucose into endogenous glycoproteins. The req... | [
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PMID:4166 | Adjuvant and immunostimulating activities of water-soluble substances extracted from Mycobacterium tuberculosis (var. hominis). | Water-soluble substances have been extracted from two strains of Mycobacterium tuberculosis var. hominis: the native hydrosoluble part (polysaccharide and peptidoglycan), a substance in which the polysaccharide moiety is less abundant than in the latter, the acetylated peptidoglycan and, finally a tetrasaccharide-hepta... | Adjuvant and immunostimulating activities of water-soluble substances extracted from Mycobacterium tuberculosis (var. hominis). Water-soluble substances have been extracted from two strains of Mycobacterium tuberculosis var. hominis: the native hydrosoluble part (polysaccharide and peptidoglycan), a substance in which ... | [
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PMID:4167 | Arylsulfatases isoenzymes in metachromatic leucodystrophy/detection of a new variant by electrophoresis improvement of quantitative assay. | Arylsulfatase A and B activities were assayed in leucocytes of 43 controls, 11 cases of Metachromatic Leucodystrophy and 7 parents or siblings of patients, using a new technique implying specific inhibitors for leucocyte enzymes. Heterozygotes can be determined, with a 50% value compared to the control, in variant B. E... | Arylsulfatases isoenzymes in metachromatic leucodystrophy/detection of a new variant by electrophoresis improvement of quantitative assay. Arylsulfatase A and B activities were assayed in leucocytes of 43 controls, 11 cases of Metachromatic Leucodystrophy and 7 parents or siblings of patients, using a new technique imp... | [
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PMID:4168 | Intrahepatic lymphoid tissue graft: course of the Gvh reaction induced by Peyer's patches. | To investigate the thymus-dependent immune competence of Peyer's patches, the course of splenomegaly and hepatic perivascular infiltration (PVI) was studied as criteria of graft-versus-host reaction (GvhR). Parental or F1 hybrid lymphoid tissues, were intrahepatically implanted and the ability of Peyer's patches to ind... | Intrahepatic lymphoid tissue graft: course of the Gvh reaction induced by Peyer's patches. To investigate the thymus-dependent immune competence of Peyer's patches, the course of splenomegaly and hepatic perivascular infiltration (PVI) was studied as criteria of graft-versus-host reaction (GvhR). Parental or F1 hybrid ... | [
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PMID:4172 | Marine sterols. III--The sterol compositions of oceanic jellyfish. The use of gas chromatographic mass spectrometric techniques to identify unresolved components. | The sterol compositions of three oceanic jellyfish have been determined using gas chromatographic mass spectrometric techniques involving the use of two separate gas chromatographic column systems. The components in overlapping peaks have been identified by comparison of the mass spectra of peaks in the two column syst... | Marine sterols. III--The sterol compositions of oceanic jellyfish. The use of gas chromatographic mass spectrometric techniques to identify unresolved components. The sterol compositions of three oceanic jellyfish have been determined using gas chromatographic mass spectrometric techniques involving the use of two sepa... | [
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PMID:4173 | The gas chromatographic mass spectrometric determination of trifluoroacetic acid in biological fluid. Application to halothane metabolism. | The methyl ester of trifluoroacetic acid was prepared by reaction with N,N'-dimethylformamide dimethylacetal and was successfully passed through a gas chromatograph. Trifluoroacetic acid was detected by the use of gas chromatography and low and high resolution gas chromatography mass spectrometry in an acidic extract o... | The gas chromatographic mass spectrometric determination of trifluoroacetic acid in biological fluid. Application to halothane metabolism. The methyl ester of trifluoroacetic acid was prepared by reaction with N,N'-dimethylformamide dimethylacetal and was successfully passed through a gas chromatograph. Trifluoroacetic... | [
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PMID:4174 | [Reciprocal influence of the graft vs. host reaction and pregnancy]. | The graft versus host reaction (GVHR) was induced in mouse females-hybrids F1 (CBA X C57BL/6) by intravenous injection of suspension of the lymphoid cells of the spleen and of lymphoid nodes from C57BL/6 mouse females. Pregnancy resulted from interbreeding of the test females with syngenic males 1--5 days before, and 1... | [Reciprocal influence of the graft vs. host reaction and pregnancy]. The graft versus host reaction (GVHR) was induced in mouse females-hybrids F1 (CBA X C57BL/6) by intravenous injection of suspension of the lymphoid cells of the spleen and of lymphoid nodes from C57BL/6 mouse females. Pregnancy resulted from interbre... | [
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PMID:4175 | Studies on gamma-glutamyl transpeptidase in human and rabbit erythrocytes. | Gamma-glutamyl transpeptidase transfers the gamma-glutamyl moiety of glutathione to a variety of acceptor amino acids. Through the operation of the gamma-glutamyl-cyclotransferase cycle, this enzyme has been implicated in the transport of amino acids into cells, especially the cells of the proximal tubules of kidney. I... | Studies on gamma-glutamyl transpeptidase in human and rabbit erythrocytes. Gamma-glutamyl transpeptidase transfers the gamma-glutamyl moiety of glutathione to a variety of acceptor amino acids. Through the operation of the gamma-glutamyl-cyclotransferase cycle, this enzyme has been implicated in the transport of amino ... | [
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PMID:4176 | The pH dependence of quantitative ristocetin-induced platelet aggregation: theoretical and practical implications-a new device for maintenance of platelet-rich plasma pH. | Quantitative ristocetin-induced platelet aggregation of normal platelet-rich plasma (PRP) decreased with time after PRP preparation. An increase in p H of the PRP with time proved to be responsible for this finding. Diffusion of CO2from the plasma is the prime determinant of the change in pH. Since a complex combinatio... | The pH dependence of quantitative ristocetin-induced platelet aggregation: theoretical and practical implications-a new device for maintenance of platelet-rich plasma pH. Quantitative ristocetin-induced platelet aggregation of normal platelet-rich plasma (PRP) decreased with time after PRP preparation. An increase in p... | [
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PMID:4177 | [Case of congenital megaurethra associated with bilateral renal hyperplasia]. | Authors report a very rare malformation of the external genital organs in a male new-born who died 48 hours after birth. This malformation consisted in a total aplasia of the corpus cavernosum and bulbus penis with a fusiforme dilatation of the anterior urethra. The malformation was associated with an anal imperforatio... | [Case of congenital megaurethra associated with bilateral renal hyperplasia]. Authors report a very rare malformation of the external genital organs in a male new-born who died 48 hours after birth. This malformation consisted in a total aplasia of the corpus cavernosum and bulbus penis with a fusiforme dilatation of t... | [
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PMID:4179 | On the mechanism of action of clozapine on the adrenergic neurone. | 1 The antipsychotic drug, clozapine, lowered noradrenaline and metaraminol (MA) concentrations in the rat heart. This action was blocked by the presence of a ganglionic blocking drug. 2 Other alpha-adrenoceptor blocking drugs (phenoxybenzamine, phentolamine) did not significantly lower heart amine concentrations. An in... | On the mechanism of action of clozapine on the adrenergic neurone. 1 The antipsychotic drug, clozapine, lowered noradrenaline and metaraminol (MA) concentrations in the rat heart. This action was blocked by the presence of a ganglionic blocking drug. 2 Other alpha-adrenoceptor blocking drugs (phenoxybenzamine, phentola... | [
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PMID:4186 | Effect of SAS (a new 10-N-acylaminophenothiazine) on gastric secretion and ulceration in rats. | The antiulcer and antisecretory activity of 2-chloro-10-[4'(N-beta-hydroxyethyl) piperazinyl-1'] acetylphenothiazine (SAS) has been investigated. At 10 and 20 mg/kg (s.c.) the drug was found to possess potent antigastric secretory and antiulcer activity (both in shay and stress ulcers) and did not exhibit any periphera... | Effect of SAS (a new 10-N-acylaminophenothiazine) on gastric secretion and ulceration in rats. The antiulcer and antisecretory activity of 2-chloro-10-[4'(N-beta-hydroxyethyl) piperazinyl-1'] acetylphenothiazine (SAS) has been investigated. At 10 and 20 mg/kg (s.c.) the drug was found to possess potent antigastric secr... | [
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PMID:4187 | Histamine H2-receptors in the human peripheral circulation. | Histamine (10 mug/min for 3 min) infused into the brachial artery caused an increase in forearm blood flow which was reduced by mepyramine (25 mg). This effect was most marked in the first minute of the infusion. Metiamide (25 mg) had no effect on the dilatation during the infusion but caused a quicker return of flow t... | Histamine H2-receptors in the human peripheral circulation. Histamine (10 mug/min for 3 min) infused into the brachial artery caused an increase in forearm blood flow which was reduced by mepyramine (25 mg). This effect was most marked in the first minute of the infusion. Metiamide (25 mg) had no effect on the dilatati... | [
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PMID:4188 | A trial of fenfluramine in the treatment of the chronic alcoholic patient. | In a double blind trial, 50 male chronic alcoholic patients were treated with either fenfluramine in a dose of 60 mg or 120 mg daily, or with identically prepared placebo tablets. Patients were interviewed on admission to the trial and then at four-weekly intervals for a period of one year and blood levels of delta-ami... | A trial of fenfluramine in the treatment of the chronic alcoholic patient. In a double blind trial, 50 male chronic alcoholic patients were treated with either fenfluramine in a dose of 60 mg or 120 mg daily, or with identically prepared placebo tablets. Patients were interviewed on admission to the trial and then at f... | [
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PMID:4189 | Towards rational therapy with monoamine oxidase inhibitors. | A rational approach to the use of monoamine oxidase inhibitors (MAOIs) is outlined. Patients suitable for treatment cannot be classified adequately using conventional diagnostic labels. They include those with primary symptoms of hypochondriasis, agoraphobia and social phobias, irritability, somatic anxiety and anergia... | Towards rational therapy with monoamine oxidase inhibitors. A rational approach to the use of monoamine oxidase inhibitors (MAOIs) is outlined. Patients suitable for treatment cannot be classified adequately using conventional diagnostic labels. They include those with primary symptoms of hypochondriasis, agoraphobia a... | [
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PMID:4190 | Urinary gamma-glutamyl transpeptidase, an indicator of renal ischaemic injury and homograft rejection. | Gammaglutamyl transpeptidase is an enzyme primarily located in the brush border of the proximal convoluted tubules of the kidney. Its unique localisation in the renal cells most easily damaged by ischaemia and its ease of assay provides the rationale for its use in the measurement of renal ischaemic injury. Using a sta... | Urinary gamma-glutamyl transpeptidase, an indicator of renal ischaemic injury and homograft rejection. Gammaglutamyl transpeptidase is an enzyme primarily located in the brush border of the proximal convoluted tubules of the kidney. Its unique localisation in the renal cells most easily damaged by ischaemia and its eas... | [
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PMID:4193 | Neutrophil pyruvate kinase deficiency with recurrent staphylococcal infections: first reported case. | A woman with an intracellular killing defect in the neutrophils had neutrophil pyruvate kinase deficiency. She had had recurrent staphylococcal infections throughout her life. The enzyme present was unstable and its kinetics were abnormal. | Neutrophil pyruvate kinase deficiency with recurrent staphylococcal infections: first reported case. A woman with an intracellular killing defect in the neutrophils had neutrophil pyruvate kinase deficiency. She had had recurrent staphylococcal infections throughout her life. The enzyme present was unstable and its kin... | [
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PMID:4197 | Tyrosine hydroxylase and dopamine-beta-hydroxylase: distribution in discrete areas of the rat limbic system. | Tyrosine hydroxylase and dopamine-beta-hydroxylase have been measured in 34 discrete areas and nuclei of the limbic system of the rat. Both enzymes showed an uneven distribution in this system. The ratio between tyrosine hydroxylase and dopamine-beta-hydroxylase activities showed a significant correlation when compared... | Tyrosine hydroxylase and dopamine-beta-hydroxylase: distribution in discrete areas of the rat limbic system. Tyrosine hydroxylase and dopamine-beta-hydroxylase have been measured in 34 discrete areas and nuclei of the limbic system of the rat. Both enzymes showed an uneven distribution in this system. The ratio between... | [
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PMID:4199 | Resolution, specificity and transphosphorylase activity of calcifying cartilage alkaline phosphatases. | The phosphate releasing activity from calf scapula cartilage was resolved by DEAE-cellulose chromatography into two distinct phosphatase activities. The activity eluted first from the column (phosphatase I) was active towards a variety of phosphate esters and several linear oligo phosphates including sodium pyrophospha... | Resolution, specificity and transphosphorylase activity of calcifying cartilage alkaline phosphatases. The phosphate releasing activity from calf scapula cartilage was resolved by DEAE-cellulose chromatography into two distinct phosphatase activities. The activity eluted first from the column (phosphatase I) was active... | [
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PMID:4200 | Testosterone metabolism in male rat epiphysis. | Using radioactive substrate, thin-layer chromatography and recrystallization methods as well as differential centrifugation, gel filtration and electrophoresis, testosterone metabolism was investigated in male rat epiphyseal growth plate. 5alpha-androstane 3alpha-17beta-diol was found to be a major metabolite in vitro;... | Testosterone metabolism in male rat epiphysis. Using radioactive substrate, thin-layer chromatography and recrystallization methods as well as differential centrifugation, gel filtration and electrophoresis, testosterone metabolism was investigated in male rat epiphyseal growth plate. 5alpha-androstane 3alpha-17beta-di... | [
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PMID:4201 | Studies related to antitumor antibiotics. Part V. Reactions of mitomycin C with DNA examined by ethidium fluorescence assay. | The cytotoxic action of the antitumor antibiotic mitomycin C occurs primarily at the level of DNA. Using highly sensitive fluorescence assays which depend on the enhancement of ethidium fluorescence only when it intercalates duplex regions of DNA, three aspects of mitomycin C action on DNA have been studied: (a) cross-... | Studies related to antitumor antibiotics. Part V. Reactions of mitomycin C with DNA examined by ethidium fluorescence assay. The cytotoxic action of the antitumor antibiotic mitomycin C occurs primarily at the level of DNA. Using highly sensitive fluorescence assays which depend on the enhancement of ethidium fluoresce... | [
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PMID:4202 | Acid proteases from species of Mucor. III. Interaction with concanavalin A and concanavalin A Sepharose. | The reaction of Mucor miehei protease with concanavalin A was followed by a turbidimetric assay in the pH range 5-8. At pH 4.0, no turbidity developed but binding of the enzyme to concanavalin A could be demonstrated by gel filtration. Two fractions of apparent molecular weight 65000 and 52000 were isolated, the 65000 ... | Acid proteases from species of Mucor. III. Interaction with concanavalin A and concanavalin A Sepharose. The reaction of Mucor miehei protease with concanavalin A was followed by a turbidimetric assay in the pH range 5-8. At pH 4.0, no turbidity developed but binding of the enzyme to concanavalin A could be demonstrate... | [
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PMID:4203 | Physical, chemical, and enzymatic studies on the major sucrase of honey bees (Apis mellifera). | A sucrase from honey bees (Apis mellifera) which precipitates between ammonium sulfate saturations of 50 and 70% (5 mg protein per millilitre) and which makes up the major portion of the sucrases of honey bees was purified to homogeneity as shown by several criteria. A large part of the sucrase was found in the head wh... | Physical, chemical, and enzymatic studies on the major sucrase of honey bees (Apis mellifera). A sucrase from honey bees (Apis mellifera) which precipitates between ammonium sulfate saturations of 50 and 70% (5 mg protein per millilitre) and which makes up the major portion of the sucrases of honey bees was purified to... | [
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PMID:4204 | Studies on the formation by rat brain preparations of CDP-diglyceride from CTP and phosphatidic acids of varying fatty acid compositions. | The enzyme, CTP:phosphatidate cytidylyltransferase (EC2.7.7.41) which catalyses formation of CDP-diglyceride from CTP and phosphatidic acid has been studied in rat brain preparations and other tissues. Improvement, as judged by the higher tissue activities obtained, in the assay method for this enzyme was achieved thro... | Studies on the formation by rat brain preparations of CDP-diglyceride from CTP and phosphatidic acids of varying fatty acid compositions. The enzyme, CTP:phosphatidate cytidylyltransferase (EC2.7.7.41) which catalyses formation of CDP-diglyceride from CTP and phosphatidic acid has been studied in rat brain preparations... | [
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PMID:4205 | Effect of metiamide on acid secretion from isolated kitten fundic mucosa. | Isolated kitten fundic mucosa demonstrates low rates of spontaneous acid secretion in vitro when bathed in Krebs-Henseleit solution, and responds consistently to histamine, pentagastrin, and acetylcholine placed in the bath. High rates of spontaneous secretion or secretion in response to histamine and pentagastrin were... | Effect of metiamide on acid secretion from isolated kitten fundic mucosa. Isolated kitten fundic mucosa demonstrates low rates of spontaneous acid secretion in vitro when bathed in Krebs-Henseleit solution, and responds consistently to histamine, pentagastrin, and acetylcholine placed in the bath. High rates of spontan... | [
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PMID:4206 | Myoplasmic impedance of the barnacle muscle fiber. | Myoplasmic impedance was measured on a barnacle (Balanus nubilus) single muscle fiber that was placed in a cylindrical cavity to limit the volume and prevent the hydration of the myoplasm. At both ends of the cavity, the myoplasm was in direct contact with an electrolyte solution. When equilibrium with the external med... | Myoplasmic impedance of the barnacle muscle fiber. Myoplasmic impedance was measured on a barnacle (Balanus nubilus) single muscle fiber that was placed in a cylindrical cavity to limit the volume and prevent the hydration of the myoplasm. At both ends of the cavity, the myoplasm was in direct contact with an electroly... | [
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PMID:4207 | Pulmonary edema and ascorbic acid loss. | Loss of ascorbic acid from lung and pulmonary edema were produced in mice by intravenous injection of either adrenaline or noradrenaline (5 mumol/kg). While adrenalectomy performed before noradrenaline administration reduced the degree of pulmonary edema, a prior dose of hexamethonium accentuated this effect. Given alo... | Pulmonary edema and ascorbic acid loss. Loss of ascorbic acid from lung and pulmonary edema were produced in mice by intravenous injection of either adrenaline or noradrenaline (5 mumol/kg). While adrenalectomy performed before noradrenaline administration reduced the degree of pulmonary edema, a prior dose of hexameth... | [
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PMID:4208 | Development of amino acid uptake activity in Neurospora. | During the germination and growth of Neurospora conidia, amino acid permease systems I (neutral) and II (general) increase in specific activity. System III (basic) decreases in specific activity with the onset of germination. System I shows two peaks of activity during the logarithmic phase of growth. One peak occurs a... | Development of amino acid uptake activity in Neurospora. During the germination and growth of Neurospora conidia, amino acid permease systems I (neutral) and II (general) increase in specific activity. System III (basic) decreases in specific activity with the onset of germination. System I shows two peaks of activity ... | [
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0.006026212591677904,... |
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