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pubmed23n0001_800
[Microbiological aspects of the performance of life support systems during prolonged operation].
The environmental control system using superoxides, sillicagels or synthetic ceolites is capable of purifying the atmosphere from microorganisms. The air conditioning system is another means for air purification from microorganisms. The possibility of microbial build-up and multiplication in the atmospheric condensate ...
[Microbiological aspects of the performance of life support systems during prolonged operation]. The environmental control system using superoxides, sillicagels or synthetic ceolites is capable of purifying the atmosphere from microorganisms. The air conditioning system is another means for air purification from microo...
1,577
pubmed23n0001_801
[Toxic properties of regenerated water containing methyl alcohol].
The experiments carried out on white male rats have shown no significant changes in the animals that may have resulted from their 30-day consumption of reclaimed water which contained up to 34 mg/l methanol and was produced by sorption purification of the atmospheric condensate during a prolonged manned experiment.
[Toxic properties of regenerated water containing methyl alcohol]. The experiments carried out on white male rats have shown no significant changes in the animals that may have resulted from their 30-day consumption of reclaimed water which contained up to 34 mg/l methanol and was produced by sorption purification of t...
1,578
pubmed23n0001_802
[Development of measures for preventing contamination of the atmosphere in hermetically sealed enclosures].
The kinetics of the outgassing of volatile substances from polymers of different function and composition was studied with respect to their natural ageing. The outgassing rate was exponentially related to the time of the sample storage. Six to nine months after the polymer fabrication the outgassing rate decreased to a...
[Development of measures for preventing contamination of the atmosphere in hermetically sealed enclosures]. The kinetics of the outgassing of volatile substances from polymers of different function and composition was studied with respect to their natural ageing. The outgassing rate was exponentially related to the tim...
1,579
pubmed23n0001_803
[Mathematical model of an experimental ecological system with spatially separated components].
The paper describes a model of an experimental ecological system consisting of autotrophic and heterotrophic components and a nutrient medium. The system has a zero stationary state and needs a correction. The life time of the system and the level of the necessary correction have been determined for the experimental ec...
[Mathematical model of an experimental ecological system with spatially separated components]. The paper describes a model of an experimental ecological system consisting of autotrophic and heterotrophic components and a nutrient medium. The system has a zero stationary state and needs a correction. The life time of th...
1,580
pubmed23n0001_804
[Control of mineral nutrition of higher plants in biological life support systems].
Control of mineral nutrition of higher plants is one of the problems involved in the development of life support systems. The experiments on carrots cultivated aeroponically have demonstrated that mineral nutrition of plants can be controlled by means of a correction solution applied in accordance with the protocols de...
[Control of mineral nutrition of higher plants in biological life support systems]. Control of mineral nutrition of higher plants is one of the problems involved in the development of life support systems. The experiments on carrots cultivated aeroponically have demonstrated that mineral nutrition of plants can be cont...
1,582
pubmed23n0001_805
[Comparative evaluation of results of intertrochanteric femur osteotomy and conservative hip joint releaving treatment in Perthes-Legg-Calvé disease].
In this paper the comparative evaluation of the results of the surgical and conservative treatment applied in patients with Perthes-Legg-Calvé disease is given. The results obtained with intertrochanteric femur osteotomy performed in 39 patients and those obtained in 66 patients with conservative treatment are reported...
[Comparative evaluation of results of intertrochanteric femur osteotomy and conservative hip joint releaving treatment in Perthes-Legg-Calvé disease]. In this paper the comparative evaluation of the results of the surgical and conservative treatment applied in patients with Perthes-Legg-Calvé disease is given. The resu...
1,587
pubmed23n0001_806
[Model of an ecosystem closed as regards gas metabolism and with a periodically working autotrophic component. II. Stability of the periodic cycles].
The literature data concerning the effect of the atmosphere composition on the photosynthetic productivity of an intensive microalgal culture were used to build a mathematical model of a semiclosed ecological system working in the light-dark alternating cycles. The type of the relationship between time cycles and compo...
[Model of an ecosystem closed as regards gas metabolism and with a periodically working autotrophic component. II. Stability of the periodic cycles]. The literature data concerning the effect of the atmosphere composition on the photosynthetic productivity of an intensive microalgal culture were used to build a mathema...
1,583
pubmed23n0001_807
[Respiratory insufficiency in severely burned patients].
The increasing number of respiratory insufficiency, as well as of pulmonary complications after burns and their role in the post-traumatic mortality are pointed out by the author. The pathophysiological processes responsible for the development of post-traumatic pulmonary complications are shortly resumed. In respect o...
[Respiratory insufficiency in severely burned patients]. The increasing number of respiratory insufficiency, as well as of pulmonary complications after burns and their role in the post-traumatic mortality are pointed out by the author. The pathophysiological processes responsible for the development of post-traumatic ...
1,588
pubmed23n0001_808
[Past and present management burns].
The management of burns consists essentially of the surgical removal of the necrotic skin layers and the substitution of the skin defects. Secondary skin transplantation does not prevent completely the cicatrization and all complications of the burn. The management of burns is partially classed among the problems of th...
[Past and present management burns]. The management of burns consists essentially of the surgical removal of the necrotic skin layers and the substitution of the skin defects. Secondary skin transplantation does not prevent completely the cicatrization and all complications of the burn. The management of burns is parti...
1,589
pubmed23n0001_809
[Skin substitution in neck and facial burns].
The surgeon assuming the task of reconstruction of burned face must be skilled in plastic surgery. Primary surgical treatment and reconstruction are inseparable, therefore it is desirable that the treatment is performed since the day of the injury to the completion of the reconstruction by the same surgeon. In the surg...
[Skin substitution in neck and facial burns]. The surgeon assuming the task of reconstruction of burned face must be skilled in plastic surgery. Primary surgical treatment and reconstruction are inseparable, therefore it is desirable that the treatment is performed since the day of the injury to the completion of the r...
1,590
pubmed23n0001_810
[Experience with the treatment of open fractures].
The number of the injuries and of the open fractures is steadily increasing. These latter are regularly due to traffic accidents. Characteristics of the open fractures are the extended lesion of the soft parts and the piece-like fractures. The treatmend depends on the patient's general state, the extension of the lesio...
[Experience with the treatment of open fractures]. The number of the injuries and of the open fractures is steadily increasing. These latter are regularly due to traffic accidents. Characteristics of the open fractures are the extended lesion of the soft parts and the piece-like fractures. The treatmend depends on the ...
1,591
pubmed23n0001_811
[Presence of foreign body in hand and foot, diagnosed too late].
On the basis of 7 years' material, the distribution of 73 foreign bodies - of non-metallic intensity - in the hand and the foot is discussed. The foreign bodies did not appear as new, fresh lesions, but they have been diagnosed after longer or shorter symptom-free state. In the case of fistulous process immediate remov...
[Presence of foreign body in hand and foot, diagnosed too late]. On the basis of 7 years' material, the distribution of 73 foreign bodies - of non-metallic intensity - in the hand and the foot is discussed. The foreign bodies did not appear as new, fresh lesions, but they have been diagnosed after longer or shorter sym...
1,592
pubmed23n0001_812
[Therapeutic principles and results in the management of open fractures of the tibial].
In open fractures of the leg, in which because of any reason no primary stable osteosynthesis can be performed, threading with Kirschner wire is preferred by the authors. This method assures adaptation stability, with minimal osteosynthesis. Primary closure of the wound in the soft parts is striven by the authors, - if...
[Therapeutic principles and results in the management of open fractures of the tibial]. In open fractures of the leg, in which because of any reason no primary stable osteosynthesis can be performed, threading with Kirschner wire is preferred by the authors. This method assures adaptation stability, with minimal osteos...
1,593
pubmed23n0001_813
[Infectious complications of primary osteosynthesis in aged patients].
In respect of iatrogenic infections the elderly patients are to be considered as endangered. Modern traumatology increases this danger in consequence of the foreign materials placed in the wounds. The role of the antibiotic and chemotherapeutic prophylaxis is of utmost importance. Regrettably, the resistance of the pyo...
[Infectious complications of primary osteosynthesis in aged patients]. In respect of iatrogenic infections the elderly patients are to be considered as endangered. Modern traumatology increases this danger in consequence of the foreign materials placed in the wounds. The role of the antibiotic and chemotherapeutic prop...
1,594
pubmed23n0001_814
[Soft tissue complications and their reconstruction following osteosynthesis of open tibial fractures].
The nowadays accepted principles of the treament of open leg fractures are discussed. The open character of the lesion multiplies the possibilities of complications. In the present paper the complications of the bone recovery are not dealt with, only the complications observed in the soft parts and the possibilities of...
[Soft tissue complications and their reconstruction following osteosynthesis of open tibial fractures]. The nowadays accepted principles of the treament of open leg fractures are discussed. The open character of the lesion multiplies the possibilities of complications. In the present paper the complications of the bone...
1,595
pubmed23n0001_815
[Experience with the treatment of open leg fractures].
Open leg fractures observed in 81 patients are analysed by the authors. In case of conservative treatment alone and in the group, which has been treated with primary osteosynthesis satisfactory results have been observed by the authors. False joint and osteomyelitis happened only in the group, in which the patients obt...
[Experience with the treatment of open leg fractures]. Open leg fractures observed in 81 patients are analysed by the authors. In case of conservative treatment alone and in the group, which has been treated with primary osteosynthesis satisfactory results have been observed by the authors. False joint and osteomyeliti...
1,596
pubmed23n0001_816
[Primary humero-radial arthrodesis].
The surgical treatment of a severe injury in the cubital region of a bus-driver, aged 47, is reported. Because of the extended contamination and the splintered fracture radical wound excision - involving also the chondral surfaces - has been performed and hereupon humero-radial arthrodesis was carried out. The skin def...
[Primary humero-radial arthrodesis]. The surgical treatment of a severe injury in the cubital region of a bus-driver, aged 47, is reported. Because of the extended contamination and the splintered fracture radical wound excision - involving also the chondral surfaces - has been performed and hereupon humero-radial arth...
1,599
pubmed23n0001_817
[Xenograft in the treatment of burns].
The injury due to burns embraces three, nearly concentric zones. The necrobiosis of the central and marginal zones is reversible and the pig-skin - assuring biological ligature - plays important role in turning for better of the process. The preparation and use of Xenograft - applied in the author's department - is dis...
[Xenograft in the treatment of burns]. The injury due to burns embraces three, nearly concentric zones. The necrobiosis of the central and marginal zones is reversible and the pig-skin - assuring biological ligature - plays important role in turning for better of the process. The preparation and use of Xenograft - appl...
1,600
pubmed23n0001_818
Mosquito-borne arboviruses in arctic america.
Mosquito-borne arboviruses are prevalent throughout subarctic regions of Canada and Alaska, principally in the boreal forest extending between latitudes 53 and 66 degrees N, but they have been identified in tundra regions as far north as 70 degrees N. All mosquito-borne agents have been bunyaviruses, comprising princip...
Mosquito-borne arboviruses in arctic america. Mosquito-borne arboviruses are prevalent throughout subarctic regions of Canada and Alaska, principally in the boreal forest extending between latitudes 53 and 66 degrees N, but they have been identified in tundra regions as far north as 70 degrees N. All mosquito-borne age...
1,602
pubmed23n0001_819
The significance of mosquito longevity and blood-feeding behaviour in the dynamics of arbovirus infections.
Mosquito longevity and blood-feeding behaviour are very important but neglected factors in the dynamics of arbovirus infections as changes in them affect transmission rates exponentially. Some mosquito species feed on a narrow range of vertebrates, some on a wide range, both influenced by host-availability and other en...
The significance of mosquito longevity and blood-feeding behaviour in the dynamics of arbovirus infections. Mosquito longevity and blood-feeding behaviour are very important but neglected factors in the dynamics of arbovirus infections as changes in them affect transmission rates exponentially. Some mosquito species fe...
1,604
pubmed23n0001_820
Properties of Semliki Forest virus nucleocapsid.
The nucleocapsid of the Semliki Forest virus is composed of 34% RNA and 66% protein, or one RNA and about 240 capsid protein molecules. The particle is spherical, with a diameter of 38--39 nm. If the nucleocapsid is exposed to slightly acid pH (6.4--5.6) it undergoes a structural change and is contracted to a 32 nm sta...
Properties of Semliki Forest virus nucleocapsid. The nucleocapsid of the Semliki Forest virus is composed of 34% RNA and 66% protein, or one RNA and about 240 capsid protein molecules. The particle is spherical, with a diameter of 38--39 nm. If the nucleocapsid is exposed to slightly acid pH (6.4--5.6) it undergoes a s...
1,605
pubmed23n0001_821
[Demonstration of T-cell activation using synthetic polynucleotides].
The present data show that SPN have a striking influence on thymus dependent T cells. The enhancing effect of gvh reactions on antibody formation against thymus dependent antigens give a good explanation for the antitumor effects of this substance.
[Demonstration of T-cell activation using synthetic polynucleotides]. The present data show that SPN have a striking influence on thymus dependent T cells. The enhancing effect of gvh reactions on antibody formation against thymus dependent antigens give a good explanation for the antitumor effects of this substance.
1,608
pubmed23n0001_822
[Stress incontinence in the woman (author's transl)].
The morphology and function of the urethrovesical junction are described to give some understanding of the pathogenesis and therapy of female stress incontinence. Conservative procedures can only be helpful in stage-1 stress incontinence, whereas in stage 2 surgical treatment provides excellent results.
[Stress incontinence in the woman (author's transl)]. The morphology and function of the urethrovesical junction are described to give some understanding of the pathogenesis and therapy of female stress incontinence. Conservative procedures can only be helpful in stage-1 stress incontinence, whereas in stage 2 surgical...
1,609
pubmed23n0001_823
Regulatory function of pyruvate dehydrogenase and the mitochondrion in lipogenesis.
The activity of pyruvate dehydrogenase from freshly isolated mitochondria was shown to be dependent upon the nutritional and metabolic state of the animal prior to sacrifice, such that mitochondria from the livers of 48 hr starved, diabetic, or high fat fed rats had lower enzyme activity than normal, chow fed rats. The...
Regulatory function of pyruvate dehydrogenase and the mitochondrion in lipogenesis. The activity of pyruvate dehydrogenase from freshly isolated mitochondria was shown to be dependent upon the nutritional and metabolic state of the animal prior to sacrifice, such that mitochondria from the livers of 48 hr starved, diab...
1,620
pubmed23n0001_824
Studies in vitro of lipogenesis in rat testicular tissue.
Testicular tissue was shown to contain the full complement of enzymes required for de novo synthesis of fatty acids. The enzymes capable of snythesizing palmitic acid from citrate, acetate, or acetyl CoA were found to be present in the soluble (cytoplasmic) fraction. These included fatty acid synthetase, acetyl CoA car...
Studies in vitro of lipogenesis in rat testicular tissue. Testicular tissue was shown to contain the full complement of enzymes required for de novo synthesis of fatty acids. The enzymes capable of snythesizing palmitic acid from citrate, acetate, or acetyl CoA were found to be present in the soluble (cytoplasmic) frac...
1,621
pubmed23n0001_825
Fatty acid synthesis from 2-14C-acetate in rat testis mitochondrial and cytosol fractions in vitro.
An in vitro system for acetate incorporation into fatty acids by the mitochondrial and the cytosol fractions of rat testis is described. The rate of incorporation of acetate into fatty acids was twice as fast with the mitochondrial as with the cytosol fraction; both systems were stimulated in the presence of adenosine ...
Fatty acid synthesis from 2-14C-acetate in rat testis mitochondrial and cytosol fractions in vitro. An in vitro system for acetate incorporation into fatty acids by the mitochondrial and the cytosol fractions of rat testis is described. The rate of incorporation of acetate into fatty acids was twice as fast with the mi...
1,622
pubmed23n0001_826
Fat metabolism in higher plants: metabolism of medium chain fatty acids.
Cell free preparations of avocado mesocarp and spinach leaf tissue rapidly convert lauryl CoA to DL-3-hydroxyl lauric acid as well as 2-, and 3-dodecanoic acids. The conversion does not occur under anaerobic conditions unless a suitable redox carrier such as ferredoxin is present. H2 18O is incorporated into the 3-hydr...
Fat metabolism in higher plants: metabolism of medium chain fatty acids. Cell free preparations of avocado mesocarp and spinach leaf tissue rapidly convert lauryl CoA to DL-3-hydroxyl lauric acid as well as 2-, and 3-dodecanoic acids. The conversion does not occur under anaerobic conditions unless a suitable redox carr...
1,623
pubmed23n0001_827
Stearoyl-CoA desaturase activity in mammary adenocarcinomas carried by C3H mice.
Transplantable mammary adenocarcinomas and livers of C3H mice fed a stock diet or a linoleate rich diet (15% corn oil) contain similar amounts of oleate (ca 3 mg/gm tissue). On feeding either a high carbohydrate, fat free or a high carbohydrate, saturated fat-containing (15% hydrogenated coconut or cottonseed oil) diet...
Stearoyl-CoA desaturase activity in mammary adenocarcinomas carried by C3H mice. Transplantable mammary adenocarcinomas and livers of C3H mice fed a stock diet or a linoleate rich diet (15% corn oil) contain similar amounts of oleate (ca 3 mg/gm tissue). On feeding either a high carbohydrate, fat free or a high carbohy...
1,624
pubmed23n0001_828
Iowa wrestling study: changes in the urinary profiles of wrestlers prior to and after competition.
During the 1973 and 1974 state high school wrestling championships, urine samples were obtained from wrestlers prior to the weigh-in, immediately before they wrestled, and immediately after the subjects had completed their match. Specific gravity, osmolarity, pH, sodium and potassium determinations, as well as qualitat...
Iowa wrestling study: changes in the urinary profiles of wrestlers prior to and after competition. During the 1973 and 1974 state high school wrestling championships, urine samples were obtained from wrestlers prior to the weigh-in, immediately before they wrestled, and immediately after the subjects had completed thei...
1,625
pubmed23n0001_829
Vasculitis with hepatitis B antigenemia: long-term observation in nine patients.
The development of generalized necrotizing vasculitis in association with hepatitis B antigenemia is the first example in man of a chronic rheumatic disease presumably caused by a viral infection. This report reviews the experience in nine biopsy-proven cases of hepatitis B-associated necrotizing vasculitis followed fo...
Vasculitis with hepatitis B antigenemia: long-term observation in nine patients. The development of generalized necrotizing vasculitis in association with hepatitis B antigenemia is the first example in man of a chronic rheumatic disease presumably caused by a viral infection. This report reviews the experience in nine...
1,628
pubmed23n0001_830
Polyarthritis, polyarteritis and hepatitis B.
An association between viral hepatitis and two rheumatic disease syndromes has been observed. Twenty-nine patients manifested a transient polyarthritis, sometimes associated with a rash (Group I). Ten patients were seen with a multisystem disease (Group II). Histologic evidence of arteritis or glomerulonephritis was pr...
Polyarthritis, polyarteritis and hepatitis B. An association between viral hepatitis and two rheumatic disease syndromes has been observed. Twenty-nine patients manifested a transient polyarthritis, sometimes associated with a rash (Group I). Ten patients were seen with a multisystem disease (Group II). Histologic evid...
1,629
pubmed23n0001_831
Some evidence for aldosterone action on 2,3-diphosphoglycerate level in human red cells.
In healthy male subjects aldosterone excretion and plasma renin activity were reduced by a 4-6 hr head-out immersion in thermoindifferent water baths (35.5 +/- 0.1 degrees C). The red cell 2,3-diphosphoglycerate (DPG) concentration before and throughout immersion period was positively correlated both with aldosterone e...
Some evidence for aldosterone action on 2,3-diphosphoglycerate level in human red cells. In healthy male subjects aldosterone excretion and plasma renin activity were reduced by a 4-6 hr head-out immersion in thermoindifferent water baths (35.5 +/- 0.1 degrees C). The red cell 2,3-diphosphoglycerate (DPG) concentration...
1,630
pubmed23n0001_832
[Alcohol dehydrogenase activity of nonsulfur purple bacteria].
Rhodopseudomonas palustris, Rh. viridis, Rh. acidophila, and Rhodomicrobium vanniellii grow on media containing ethanol, n-propanol, and n-butanol. The highest amount of lower alcohols is utilized by the strains of Rh. palustris. Only Rh. acidophila accumulates methanol. Alcohol dehydrogenase of Rh. palustris, Rh. viri...
[Alcohol dehydrogenase activity of nonsulfur purple bacteria]. Rhodopseudomonas palustris, Rh. viridis, Rh. acidophila, and Rhodomicrobium vanniellii grow on media containing ethanol, n-propanol, and n-butanol. The highest amount of lower alcohols is utilized by the strains of Rh. palustris. Only Rh. acidophila accumul...
1,631
pubmed23n0001_833
[Development of Mycobacterium lacticolum and its synthesis of exopolysaccharide under conditions of varying acidity of the medium].
Mycobacterium lacticolum 121 grows and synthesizes exopolysaccharide at the initial values of pH of the medium from 5.0 to 11.5. The accumulation of biomass is maximal (10 to 12 gram/litre) at pH 6.0 to 11.5; the rate of growth is highest at pH 8.0 to 11.5. The largest amount of exopolysaccharide (2.0 to 2.4 gram/litre...
[Development of Mycobacterium lacticolum and its synthesis of exopolysaccharide under conditions of varying acidity of the medium]. Mycobacterium lacticolum 121 grows and synthesizes exopolysaccharide at the initial values of pH of the medium from 5.0 to 11.5. The accumulation of biomass is maximal (10 to 12 gram/litre...
1,632
pubmed23n0001_834
[Effect of H+ and OH- ions on the physiological and biochemical properties of a Pseudomonas methanolica culture].
The growth of Pseudomonas methanolica was inhibited by unfavourable values of pH in the conditions of chemostat; the rate of the substrate assimilation was higher, and anabolic and catabolic reactions were decoupled. Hydrogen ions inhibited the activity of enzymes of the Krebs cycle, hydroxyl ions inhibited the activit...
[Effect of H+ and OH- ions on the physiological and biochemical properties of a Pseudomonas methanolica culture]. The growth of Pseudomonas methanolica was inhibited by unfavourable values of pH in the conditions of chemostat; the rate of the substrate assimilation was higher, and anabolic and catabolic reactions were ...
1,633
pubmed23n0001_835
[Panarteritis nodosa-Special aspects of glucocorticoid and immune suppressive therapy (author's transl)].
Report dealing with the clinical and pathoanatomical course as well as the autopsy findings in a 54 year old female suffering from panarteritis nodosa. Onset of the illness with polyneuritis and arthralgia. One year later diagnosis of panarteritis nodosa verified by muscle biopsy. Deterioration of the disease leading t...
[Panarteritis nodosa-Special aspects of glucocorticoid and immune suppressive therapy (author's transl)]. Report dealing with the clinical and pathoanatomical course as well as the autopsy findings in a 54 year old female suffering from panarteritis nodosa. Onset of the illness with polyneuritis and arthralgia. One yea...
1,639
pubmed23n0001_836
[Factors causing lysis of an Actinomyces rimosus culture, the producer of the antibiotic, oxytetracycline].
The lysis of Actinomyces rimosus producing oxytetracycline during its mass growth can be caused by two factors which were separated by differential centrifugation. The first factor is phage particles of a temperate phage produced by the culture; they are incapable of growth but may induce the lysis. The phage particles...
[Factors causing lysis of an Actinomyces rimosus culture, the producer of the antibiotic, oxytetracycline]. The lysis of Actinomyces rimosus producing oxytetracycline during its mass growth can be caused by two factors which were separated by differential centrifugation. The first factor is phage particles of a tempera...
1,634
pubmed23n0001_837
[Pulmonary vascular pressures and blood gases in young patients with thoracic scoliosis (author's transl)].
Pulmonary artery and pulmonary wedge pressures were measured at rest and during exercise in 25 young patients with moderate to severe scoliosis. Simultaneous determinations of arterial blood gases and pH were performed. The results of the vascular pressure measurements were compared with those obtained in 15 healthy yo...
[Pulmonary vascular pressures and blood gases in young patients with thoracic scoliosis (author's transl)]. Pulmonary artery and pulmonary wedge pressures were measured at rest and during exercise in 25 young patients with moderate to severe scoliosis. Simultaneous determinations of arterial blood gases and pH were per...
1,638
pubmed23n0001_838
Role of hyperkalemia in the metabolic acidosis of isolated hypoaldosteronism.
We studied the relative importance of hyperkalemia and mineralocorticoid deficiency in the metabolic acidosis of a patient with proved isolated hyporeninemic hypoaldosteronism and moderate kidney failure. The hyperkalemia and acidosis were severe in relation to the slight azotemia. Despite the systemic acidosis and uri...
Role of hyperkalemia in the metabolic acidosis of isolated hypoaldosteronism. We studied the relative importance of hyperkalemia and mineralocorticoid deficiency in the metabolic acidosis of a patient with proved isolated hyporeninemic hypoaldosteronism and moderate kidney failure. The hyperkalemia and acidosis were se...
1,674
pubmed23n0001_839
Isolation of Penicillium corylophium Dierckx from acid mine water and its optimal growth on hydrocarbons at acid pH.
Penicillium corylophilium Dieckx was isolated from sludge collected at the interface of an aqueous, copper-bearing leachate and an organic, kerosene based, ion exchange solvent. The organism assimilated kerosene and various straight chain and cyclic hydrocarbons including dodecane, hexadecane, octadecane, toluene, benz...
Isolation of Penicillium corylophium Dierckx from acid mine water and its optimal growth on hydrocarbons at acid pH. Penicillium corylophilium Dieckx was isolated from sludge collected at the interface of an aqueous, copper-bearing leachate and an organic, kerosene based, ion exchange solvent. The organism assimilated ...
1,676
pubmed23n0001_840
The effect of microbial mycolytic agents on Trichophyton rubrum.
Chitinolytic microorganisms isolated from forest soil and from healthy gypsy moth larvae (Porthetria dispar (L.) were screened for their ability to lyse Trichophyton rubrum mycelia. A few of these isolates were mycolytic on both autoclaved and on actively growing, intact, T. rubrum mycelia. Supernatants from these isol...
The effect of microbial mycolytic agents on Trichophyton rubrum. Chitinolytic microorganisms isolated from forest soil and from healthy gypsy moth larvae (Porthetria dispar (L.) were screened for their ability to lyse Trichophyton rubrum mycelia. A few of these isolates were mycolytic on both autoclaved and on actively...
1,677
pubmed23n0001_841
Models for metal ion function in carbonic anhydrase.
A model catalyst is described which has properties in common with carbonic anhydrase. The model demonstrates the availability of a mechanism, previously only hypothetical, for the action of the enzyme. It also shows, however, that this mechanism alone is not adequate to produce the high activity of the enzyme.
Models for metal ion function in carbonic anhydrase. A model catalyst is described which has properties in common with carbonic anhydrase. The model demonstrates the availability of a mechanism, previously only hypothetical, for the action of the enzyme. It also shows, however, that this mechanism alone is not adequate...
1,680
pubmed23n0001_842
[Regulation of lipid synthesis in animal organs].
Studies were made of the mechanisms regulating the quantity and catalytic efficiency of hepatic acetyl coenzyme A carboxylase, which plays a critical role in the control of fatty acid biosynthesis. The microsomal enzyme system responsible for the formation of phosphatidic acid, the initial step in glycerolipid biosynth...
[Regulation of lipid synthesis in animal organs]. Studies were made of the mechanisms regulating the quantity and catalytic efficiency of hepatic acetyl coenzyme A carboxylase, which plays a critical role in the control of fatty acid biosynthesis. The microsomal enzyme system responsible for the formation of phosphatid...
1,682
pubmed23n0001_843
Comparison of properties between virulent and attenuated strains of transmissible gastroenteritis virus.
Strains of transmissible gastroenteritis (TGE) virus possessing different pathogenicity were examined for stability to digestive enzymes and acid, and growth at various temperatures. In growth experiments, virus titer obtained at 37 degrees C were about equal between attenuated and virulent strains, but titers attained...
Comparison of properties between virulent and attenuated strains of transmissible gastroenteritis virus. Strains of transmissible gastroenteritis (TGE) virus possessing different pathogenicity were examined for stability to digestive enzymes and acid, and growth at various temperatures. In growth experiments, virus tit...
1,684
pubmed23n0001_844
Growth of Ibaraki virus in suspension culture of HmLu-1 cells.
The established hamster lung cell line, HmLu-1 cells could grow in a suspended state. The initial cell count, 40 X 10(4)/ml, increased to 200 X 10(4)/ml on the 4th day of culture. The suspension culture of HmLu-1 cells was proved satisfactory for propagation of Ibaraki virus. The viral titer reached a maximum of 10(6.7...
Growth of Ibaraki virus in suspension culture of HmLu-1 cells. The established hamster lung cell line, HmLu-1 cells could grow in a suspended state. The initial cell count, 40 X 10(4)/ml, increased to 200 X 10(4)/ml on the 4th day of culture. The suspension culture of HmLu-1 cells was proved satisfactory for propagatio...
1,685
pubmed23n0001_845
Studies on the mechanism of the action of morphine on the peristalsis of guinea pig ileum in situ.
The influence of some drugs on the effect of morphine on the threshold pressure required to elicit peristalsis in the guinea pig ileum in situ was studied, in order to test the hypothesis that this effect of morphine is mediated by catecholamine release. Tachyphylaxis to this effect of morphine was confirmed. Pretreate...
Studies on the mechanism of the action of morphine on the peristalsis of guinea pig ileum in situ. The influence of some drugs on the effect of morphine on the threshold pressure required to elicit peristalsis in the guinea pig ileum in situ was studied, in order to test the hypothesis that this effect of morphine is m...
1,686
pubmed23n0001_846
Effects of alpha-ketomonocarboxylic acids upon insulin secretion and metabolism of isolated pancreatic islets.
In perifused isolated pancreatic islets alpha-ketoisocaproic acid (KIC) or alpha-ketocaproic acid (KC) induced a high insulin secretion rate and a steep increase of the fluorescence of reduced pyridine pyridine nucleotides [NAD(P)H] which fell again to almost prestimulatory levels 6 min after medium change. Insulin rel...
Effects of alpha-ketomonocarboxylic acids upon insulin secretion and metabolism of isolated pancreatic islets. In perifused isolated pancreatic islets alpha-ketoisocaproic acid (KIC) or alpha-ketocaproic acid (KC) induced a high insulin secretion rate and a steep increase of the fluorescence of reduced pyridine pyridin...
1,687
pubmed23n0001_847
The preparation and properties of nerve growth factor protein at alkaline pH.
The nerve growth factor (NGF) subunit of 7S NGF was isolated by chromatography at high pH on QAE-Sephadex. It has the same specific NGF activity as betaNGF isolated at acid pH, showing that this activity is an intrinsic property of the subunit and is independent of the pathway of dissociation. Continued exposure of the...
The preparation and properties of nerve growth factor protein at alkaline pH. The nerve growth factor (NGF) subunit of 7S NGF was isolated by chromatography at high pH on QAE-Sephadex. It has the same specific NGF activity as betaNGF isolated at acid pH, showing that this activity is an intrinsic property of the subuni...
1,689
pubmed23n0001_848
Concanavalin A-binding glycopeptides from rat brain glycoproteins.
The affinity of concanavalin A for neutral and acidic glycopeptides derived from rat brain glycoproteins was investigated by studying the inhibition of a concanavalin A-glycogen precipitation system. The neutral, mannose-rich glycopeptides obtained by column electrophoresis of the dialyzable glycopeptides that had been...
Concanavalin A-binding glycopeptides from rat brain glycoproteins. The affinity of concanavalin A for neutral and acidic glycopeptides derived from rat brain glycoproteins was investigated by studying the inhibition of a concanavalin A-glycogen precipitation system. The neutral, mannose-rich glycopeptides obtained by c...
1,690
pubmed23n0001_849
The use of hybrid molecules in a study of the equilibrium between nerve growth factor monomers and dimers.
The major protein in beta nerve growth factor preparations, beta1NGF, is a dimer in which both peptide chains have COOH-terminal arginine residues. Digestion of beta1NGF with carboxypeptidase B produced a dimer, beta3NGF, in which both chains lack these terminal arginine residues. Exposure of mixtures of beta1 and beta...
The use of hybrid molecules in a study of the equilibrium between nerve growth factor monomers and dimers. The major protein in beta nerve growth factor preparations, beta1NGF, is a dimer in which both peptide chains have COOH-terminal arginine residues. Digestion of beta1NGF with carboxypeptidase B produced a dimer, b...
1,691
pubmed23n0001_850
Changes in hypothalamic neurotransmitter uptake following pinealectomy, superior cervical ganglionectomy or melatonin administration to rats.
Rats subjected to pinealectomy or to pinealectomy plus bilateral superior cervical ganglionectomy exhibited depressed serotonin uptake by hypothalamic synaptosomes; norepinephrine, dopamine or glutamate uptake was not affected by any of the surgical procedures. Treatment with melatonin resulted in inhibition of hypotha...
Changes in hypothalamic neurotransmitter uptake following pinealectomy, superior cervical ganglionectomy or melatonin administration to rats. Rats subjected to pinealectomy or to pinealectomy plus bilateral superior cervical ganglionectomy exhibited depressed serotonin uptake by hypothalamic synaptosomes; norepinephrin...
1,692
pubmed23n0001_851
Timolol maleate (Blocadren) in the treatment of essential hypertension.
The antihypertensive activity of timolol (Blocadren, FROSST-MSD), a new beta blocking agent, was assessed in a single blind crossover placebo-controlled study in 14 patients with essential hypertension. Ten patients completed the trial. The average standing pressure during the placebos periods was 168/109mmHg and durin...
Timolol maleate (Blocadren) in the treatment of essential hypertension. The antihypertensive activity of timolol (Blocadren, FROSST-MSD), a new beta blocking agent, was assessed in a single blind crossover placebo-controlled study in 14 patients with essential hypertension. Ten patients completed the trial. The average...
1,695
pubmed23n0001_852
Effects of ritodrine hydrochloride on uterine activity and the cardiovascular system in toxemic patients.
The effects of ritodrine hydrochloride were evaluated in 25 toxemic patients in active labor utilizing continuous electronic monitoring of fetal and maternal cardiovascular systems and uterine activity. Fetal scalp blood and free flowing maternal antecubital venous blood was obtained for pH, Po2, Pco2, base deficit and...
Effects of ritodrine hydrochloride on uterine activity and the cardiovascular system in toxemic patients. The effects of ritodrine hydrochloride were evaluated in 25 toxemic patients in active labor utilizing continuous electronic monitoring of fetal and maternal cardiovascular systems and uterine activity. Fetal scalp...
1,696
pubmed23n0001_853
Treatment of ovarian hyperstimulation syndrome by antihistamine.
Ovarian hyperstimulation was produced by human menopausal gonadotropin and chorionic gonadotropin in rabbits. A more rapid regression of the hyperstimualted ovaries was observed in an antihistamine-treated group than in a control group. The difference in regression was found to be statistically significant. The possibi...
Treatment of ovarian hyperstimulation syndrome by antihistamine. Ovarian hyperstimulation was produced by human menopausal gonadotropin and chorionic gonadotropin in rabbits. A more rapid regression of the hyperstimualted ovaries was observed in an antihistamine-treated group than in a control group. The difference in ...
1,697
pubmed23n0001_854
High pH ammonia toxicity, and the search for life on the Jovian planets.
Jovian plants have enviroments apparently suitable for the evolution of life, but nevertheless, present severe challenges to organisms. One such challenge arises from the presence of ammonia. Ammonia is an efficient biocide, its effect being dependent on pH as well as on concentration. The effects of pH and ammonia con...
High pH ammonia toxicity, and the search for life on the Jovian planets. Jovian plants have enviroments apparently suitable for the evolution of life, but nevertheless, present severe challenges to organisms. One such challenge arises from the presence of ammonia. Ammonia is an efficient biocide, its effect being depen...
1,698
pubmed23n0001_855
Gamma-glutamyltranspeptidase in the rat liver after portacaval shunt.
Gamma-Glutamyltranspeptidase (GGTP) activity was studied in livers of rats submitted to an end-to-side portacaval shunt (PCS) and in developing animals. To correlate the evolution of the enzymatic activity measured in vitro, histochemical techniques were used to localize enzyme activity in liver tissue. The GGTP activi...
Gamma-glutamyltranspeptidase in the rat liver after portacaval shunt. Gamma-Glutamyltranspeptidase (GGTP) activity was studied in livers of rats submitted to an end-to-side portacaval shunt (PCS) and in developing animals. To correlate the evolution of the enzymatic activity measured in vitro, histochemical techniques ...
1,703
pubmed23n0001_856
Cardiovascular effects of electrical stimulation of the forebrain in the fetal lamb.
Modified stereotaxic techniques were applied to fetal lambs during the latter third of gestation. Electrical stimulation in the region of the hypothalamus in 10 acute experiments was associated with three patterns of arterial blood pressure and heart rate changes: a pressor-tachycardia response; a pure tachycardia resp...
Cardiovascular effects of electrical stimulation of the forebrain in the fetal lamb. Modified stereotaxic techniques were applied to fetal lambs during the latter third of gestation. Electrical stimulation in the region of the hypothalamus in 10 acute experiments was associated with three patterns of arterial blood pre...
1,704
pubmed23n0001_857
Elevated levels of immunoglobulin E in the acute febrile mucocutaneous lymph node syndrome.
Mucocutaneous lymph node syndrome (MCLS) is a newly recognized disease characterized by fever persisting for more than 5 days, an erythematous skin eruption, conjunctival congestion, dry red fissured lips, reddened tongue, palms, and soles, nonpurulent lymphadenopathy, and sometines diarrhea, arthralgia, and aseptic me...
Elevated levels of immunoglobulin E in the acute febrile mucocutaneous lymph node syndrome. Mucocutaneous lymph node syndrome (MCLS) is a newly recognized disease characterized by fever persisting for more than 5 days, an erythematous skin eruption, conjunctival congestion, dry red fissured lips, reddened tongue, palms...
1,705
pubmed23n0001_858
Diminished pulmonary lecithin synthesis in acidosis: experimental findings as related to the respiratory distress syndrome.
Lung slices from term fetal rats were incubated in vitro at various pH values and the rates of the two de novo pathways for lecithin biosynthesis were determined by measuring the conversion of either 14C-choline (pathway 1) or 14C-methionine (pathway 2) to the phospholipid. It was observed that the choline pathway, but...
Diminished pulmonary lecithin synthesis in acidosis: experimental findings as related to the respiratory distress syndrome. Lung slices from term fetal rats were incubated in vitro at various pH values and the rates of the two de novo pathways for lecithin biosynthesis were determined by measuring the conversion of eit...
1,706
pubmed23n0001_859
pH and bicarbonate excretion in the rat parotid gland as a function of salivary rate.
The bicarbonate concentration in rat parotid saliva increases with increasing flow rates and approximates plasma values at highest salivation. At lowest flow rates the bicarbonate concentration in the secretory fluid markedly exceeds the plasma levels. Intravenous administration of acetazolamide has no influence on the...
pH and bicarbonate excretion in the rat parotid gland as a function of salivary rate. The bicarbonate concentration in rat parotid saliva increases with increasing flow rates and approximates plasma values at highest salivation. At lowest flow rates the bicarbonate concentration in the secretory fluid markedly exceeds ...
1,707
pubmed23n0001_860
H+ transport and membrane-bound HCO - 3 ATPase in salivary duct epithelium.
An ATPase stimulated by HCO - ions and other oxybases and inhibited by SCN- has been found in main excretory duct of rat submaxillary gland, a tissue, capable of actively secreting HCO - 3 ions. No such ATPase was found in the rabbit duct, which normally does not secrete HCO - 3. The HCO - 3 ATPase was localized in th...
H+ transport and membrane-bound HCO - 3 ATPase in salivary duct epithelium. An ATPase stimulated by HCO - ions and other oxybases and inhibited by SCN- has been found in main excretory duct of rat submaxillary gland, a tissue, capable of actively secreting HCO - 3 ions. No such ATPase was found in the rabbit duct, whi...
1,708
pubmed23n0001_861
Effects of cyanide and doxapram during hypothermia.
The ventilatory responses, blood gases and acid-base status to intravenous injections of KCN and doxapram hydrochloride were studied in anesthetized dogs during normothermia and at two levels of hypothermia. In the normothermic animal, KCN evoked significant elevations of minute and alveolar ventilations. For the mildl...
Effects of cyanide and doxapram during hypothermia. The ventilatory responses, blood gases and acid-base status to intravenous injections of KCN and doxapram hydrochloride were studied in anesthetized dogs during normothermia and at two levels of hypothermia. In the normothermic animal, KCN evoked significant elevation...
1,709
pubmed23n0001_862
Effects of cyanide and doxapram during panting.
The ventilatory responses, blood gases and acid-base status to intravenous injections of KCN and doxapram hydrochloride were studied in anesthetized dogs during normothermia and thermally induced panting. In the normothermic animal, KCN evoked elevation of VE (154.7%), VT (70.1%), f (48.3%, PaO2 (12.1%) and pH (0.098 u...
Effects of cyanide and doxapram during panting. The ventilatory responses, blood gases and acid-base status to intravenous injections of KCN and doxapram hydrochloride were studied in anesthetized dogs during normothermia and thermally induced panting. In the normothermic animal, KCN evoked elevation of VE (154.7%), VT...
1,710
pubmed23n0001_863
The effect of alpha-adrenolytics on the levels of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in rat's brain.
Alpha-Adrenolytics (ABA)-phenoxybenzamine, phentolamine and aceperone-increase 5-hydroxyindoleacetic (5-HIAA) acid content in rat's brain. At the same time, these compounds either increase or do not affect 5-hydroxytryptamine (5-HT) level. Moreover, they potentiate L-dopa and reserpine-induced increase in 5-HIAA level ...
The effect of alpha-adrenolytics on the levels of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in rat's brain. Alpha-Adrenolytics (ABA)-phenoxybenzamine, phentolamine and aceperone-increase 5-hydroxyindoleacetic (5-HIAA) acid content in rat's brain. At the same time, these compounds either increase or do not affe...
1,713
pubmed23n0001_864
Combating diabetic ketoacidosis and other hyperglycemic-ketoacidotic syndromes.
Diabetic ketoacidosis is an acute medical emergency that requires immediate diagnosis and treatment. Diagnosis may be established rapidly by measurement of urinary glucose and ketones, arterial blood pH and blood gases, and serum ketones. Rapid infusion of large volumes of fluids and electrolytes, together with continu...
Combating diabetic ketoacidosis and other hyperglycemic-ketoacidotic syndromes. Diabetic ketoacidosis is an acute medical emergency that requires immediate diagnosis and treatment. Diagnosis may be established rapidly by measurement of urinary glucose and ketones, arterial blood pH and blood gases, and serum ketones. R...
1,717
pubmed23n0001_865
[Current conception of the Bohr effect].
The molecular mechanism of the Bohr effect is explained according to the molecular model proposed by Perutz et al. The Bohr effect is due to changes in the pK of specific carboxyl and amino groups of the four globin chains following the transition between the deoxy and oxy conformations of the molecule. Carbon dioxide ...
[Current conception of the Bohr effect]. The molecular mechanism of the Bohr effect is explained according to the molecular model proposed by Perutz et al. The Bohr effect is due to changes in the pK of specific carboxyl and amino groups of the four globin chains following the transition between the deoxy and oxy confo...
1,718
pubmed23n0001_866
The central action of drugs influencing beta-adrenergic receptor. part V. The interaction of drugs affecting beta-adrenergic receptor in motility test.
Propranolol, alprenolol and sotalol given intraventricularly (ivc) to rats reduce depressing effects of isoprenaline (IPS) and antagonize stimulating effects of noradrenaline (NA). Phentolamine given by the same route of administration does not affect IPS action.
The central action of drugs influencing beta-adrenergic receptor. part V. The interaction of drugs affecting beta-adrenergic receptor in motility test. Propranolol, alprenolol and sotalol given intraventricularly (ivc) to rats reduce depressing effects of isoprenaline (IPS) and antagonize stimulating effects of noradre...
1,714
pubmed23n0001_867
[Study of P50 in patients under continuous O2 inhalation and during chronic respiratory acidosis].
In vitro, the affinity of Hb for O2 depends on pH and capnia by the intermediate of the 2-3 DPG level, the concentration of which lowers in the case of acidosis and hypercapnia. Thus, an increase in the affinity results, but while Bohr's effect is immediate, on the contrary the 2-3 DPG effect is slow. Authors have veri...
[Study of P50 in patients under continuous O2 inhalation and during chronic respiratory acidosis]. In vitro, the affinity of Hb for O2 depends on pH and capnia by the intermediate of the 2-3 DPG level, the concentration of which lowers in the case of acidosis and hypercapnia. Thus, an increase in the affinity results, ...
1,719
pubmed23n0001_868
The effect of alpha, alpha1-dipyridyl on noradrenaline, dopamine and 5-hydroxytryptamine levels and on dopamine-beta-hydroxylase activity in brain.
The effects of alpha, alpha1-dipyridyl (DP) on noradrenaline (NA), dopamine (DA) and 5-hydroxytryptamine (5-HT) levels in rat and mouse brain and on dopamine-beta-hydroxylase (DbetaH) activity in rat brain have been studied, DP decreases NA level and inhibits DbetaH activity in a dose dependent manner, without affectin...
The effect of alpha, alpha1-dipyridyl on noradrenaline, dopamine and 5-hydroxytryptamine levels and on dopamine-beta-hydroxylase activity in brain. The effects of alpha, alpha1-dipyridyl (DP) on noradrenaline (NA), dopamine (DA) and 5-hydroxytryptamine (5-HT) levels in rat and mouse brain and on dopamine-beta-hydroxyla...
1,715
pubmed23n0001_869
Colorimetric determination of bromisoval and carbromal.
New colorimetric procedure for determination of bromisoval and carbromal is based on the hydroxamation reaction and colour developing after addition of Fe(ClO4)3. The method is employed for the estimation of both compounds in substances and in tablets.
Colorimetric determination of bromisoval and carbromal. New colorimetric procedure for determination of bromisoval and carbromal is based on the hydroxamation reaction and colour developing after addition of Fe(ClO4)3. The method is employed for the estimation of both compounds in substances and in tablets.
1,716
pubmed23n0001_870
[Effect of cultivation conditions on the synthesis of citric and isocitric acids in Candida lipolytica on hexadecane medium].
The influence of aeration, pH and iron concentration on the growth of yeast C. lipolytica 704 on the hexadecane medium and on the synthesis of citric and isocitric acids was investigated. The yeast synthesized citric acids actively during intensive aeration. The acid formation was strongly dependent on the medium acidi...
[Effect of cultivation conditions on the synthesis of citric and isocitric acids in Candida lipolytica on hexadecane medium]. The influence of aeration, pH and iron concentration on the growth of yeast C. lipolytica 704 on the hexadecane medium and on the synthesis of citric and isocitric acids was investigated. The ye...
1,720
pubmed23n0001_871
[Study of inosine transformation into 5'-inosinic acid by the culture of Pseudomonas trifoli].
The transformation of inosine into 5'-inosine acid by Pseudomonas trifolii cells was studied. The synthesis of 5'-inosine acid can be performed by both live intact and dry cells. The effectiveness of inosine phosphorylation depends on the ratio of the inosine and phosphate donor concentrations and the amount of cells. ...
[Study of inosine transformation into 5'-inosinic acid by the culture of Pseudomonas trifoli]. The transformation of inosine into 5'-inosine acid by Pseudomonas trifolii cells was studied. The synthesis of 5'-inosine acid can be performed by both live intact and dry cells. The effectiveness of inosine phosphorylation d...
1,721
pubmed23n0001_872
[Effect of pH on the enzymic activity of the fungi Trichothecium roseum and Aspergillus niger hydrolyzing nonstarch polysaccharides].
The purpose of the study was to determine optimal pH values for the enzymic activity of the fungi Trichothecium roseum and Aspergillus niger hydrolyzing nonstarch polysaccharides of barley and disrupting cell walls (cytolysis) of grain, the so called cytolytic enzymes. The effect of the acidity of the medium on the sta...
[Effect of pH on the enzymic activity of the fungi Trichothecium roseum and Aspergillus niger hydrolyzing nonstarch polysaccharides]. The purpose of the study was to determine optimal pH values for the enzymic activity of the fungi Trichothecium roseum and Aspergillus niger hydrolyzing nonstarch polysaccharides of barl...
1,722
pubmed23n0001_873
[Study of the yeast dissolving enzymic complex by isoelectric focusing].
By isoelectric focussing the lyzing complex produced by Actinomyces griseinus-11 has been fractionated. The sole neutral protease which is active at pH 7.0 does not participate in lysis induced by other enzymes. The lyzing activity of this complex is associated with the carbohydrase enzymes that are at least three in n...
[Study of the yeast dissolving enzymic complex by isoelectric focusing]. By isoelectric focussing the lyzing complex produced by Actinomyces griseinus-11 has been fractionated. The sole neutral protease which is active at pH 7.0 does not participate in lysis induced by other enzymes. The lyzing activity of this complex...
1,723
pubmed23n0001_874
[Isolation and basic properties of thiamine pyrophosphokinase from brewing yeast].
Thiamine pyrophosphokinase (EC 2.7.7.2) isolated from dry brewing yeast has been purified 20-fold with a 70% yield. Certain properties of the enzyme have been determined: pH and temperature optima, donor and acceptor concentrations, and relationship between the rate of cocarboxylase biosynthesis and the incubation time...
[Isolation and basic properties of thiamine pyrophosphokinase from brewing yeast]. Thiamine pyrophosphokinase (EC 2.7.7.2) isolated from dry brewing yeast has been purified 20-fold with a 70% yield. Certain properties of the enzyme have been determined: pH and temperature optima, donor and acceptor concentrations, and ...
1,724
pubmed23n0001_875
[Trypsin interaction with sodium alginate].
The formation of an insoluble product of the interaction of trypsin and sodium alginate at pH 3-9 was studied. The optic density of the system was in an extreme relation to the composition. The insoluble phase was enriched in trypsin. The dispersion phase formed as a result of the electrostatic interaction between algi...
[Trypsin interaction with sodium alginate]. The formation of an insoluble product of the interaction of trypsin and sodium alginate at pH 3-9 was studied. The optic density of the system was in an extreme relation to the composition. The insoluble phase was enriched in trypsin. The dispersion phase formed as a result o...
1,725
pubmed23n0001_876
[Study of properties of heat activated enzyme preparations].
The paper describes studies of heat treatment of the preparations amylorizin G10x, amylosubtilin G10x and glucoendomycopsin G15 and its effect on the relationshop between the rate of the enzymic reaction and the preparation concentration, optimal pH and temperatures, and the EDTA influence on the activity of heated pre...
[Study of properties of heat activated enzyme preparations]. The paper describes studies of heat treatment of the preparations amylorizin G10x, amylosubtilin G10x and glucoendomycopsin G15 and its effect on the relationshop between the rate of the enzymic reaction and the preparation concentration, optimal pH and tempe...
1,726
pubmed23n0001_877
[Effect of pH on the properties of the chemostatic culture of Canida utilis].
The effect of hydrogen and hydroxyl ions on the physiological features of the yeast C. utilis VKMU-1668 was studied. High acidity inhibited yeast growth and uncoupled pathways of the energy and constructive metabolism: normal respiration was disturbed and the electron transport chain was damaged in the site of cytochro...
[Effect of pH on the properties of the chemostatic culture of Canida utilis]. The effect of hydrogen and hydroxyl ions on the physiological features of the yeast C. utilis VKMU-1668 was studied. High acidity inhibited yeast growth and uncoupled pathways of the energy and constructive metabolism: normal respiration was ...
1,727
pubmed23n0001_878
[Activation of L-amino acids by aminoacyl-tRNA-synthetases from yeast Candida utilis IBPM-405].
The procedure for isolating aminoacyl-tRNA-synthetases from yeast Candida utilis IBPM-405 was developed. The rate of activation of L-amino acids in the formation of hydroxamates was different. Aspartic acid, asparagine, glutamic acid, tryptophane, phenyl alanine and methionine underwent the highest activation. The acti...
[Activation of L-amino acids by aminoacyl-tRNA-synthetases from yeast Candida utilis IBPM-405]. The procedure for isolating aminoacyl-tRNA-synthetases from yeast Candida utilis IBPM-405 was developed. The rate of activation of L-amino acids in the formation of hydroxamates was different. Aspartic acid, asparagine, glut...
1,729
pubmed23n0001_879
[Properties of immobilized trypsin and alpha-chymotrypsin and their use for purification of proteinase inhibitors from potatoes].
Immobilized trypsin and alpha-chymotrypsin were obtained as a result of the enzyme attachment to bromo-cyanogen activated cepharose. Proteolytic activity (substrate--casein) of immobilized trypsin and alpha-chymotrypsin was 18.7 and 9%, respectively and their esterase activity with methyl ester benzoyl-L-arginine (tryp...
[Properties of immobilized trypsin and alpha-chymotrypsin and their use for purification of proteinase inhibitors from potatoes]. Immobilized trypsin and alpha-chymotrypsin were obtained as a result of the enzyme attachment to bromo-cyanogen activated cepharose. Proteolytic activity (substrate--casein) of immobilized t...
1,733
pubmed23n0001_880
[Some properties of proteases of the mould Mucor pusillus-917].
The proteolytic preparation of Mucor pusillus-917 has been obtained. The preparation produces an effective hydrolytic influence on milk casein. Thermal and acid inactivation of the proteolytic complex is the reaction of the first order. The activity-pH curves, calculations of ionization heat, inactivation of proteases ...
[Some properties of proteases of the mould Mucor pusillus-917]. The proteolytic preparation of Mucor pusillus-917 has been obtained. The preparation produces an effective hydrolytic influence on milk casein. Thermal and acid inactivation of the proteolytic complex is the reaction of the first order. The activity-pH cur...
1,731
pubmed23n0001_881
[Effect of different pH values on the activity and quaternary structure of asparaginase in Escherichia coli extracts].
The effect of low pH values on the activity and stability of the quaternary structure of asparaginase from Escherichia coli was investigated at early stages of purification of the enzyme. Acidification of the E. coli extract was most effective before the biomass separation. This procedure helped to separate biomass tog...
[Effect of different pH values on the activity and quaternary structure of asparaginase in Escherichia coli extracts]. The effect of low pH values on the activity and stability of the quaternary structure of asparaginase from Escherichia coli was investigated at early stages of purification of the enzyme. Acidification...
1,737
pubmed23n0001_882
[Preparation of proteolytic enzymes from the thermophilous actinomycete Actinomyces thermovulgaris str. T-54].
A preparation with a high proteolytic activity has been obtained by acetone precipitation from the culture liquid filtrate of the thermophilous actinomycete Actinomyces thermovulgaris str. T-54. The proteolytic (caseinolytic), fibrinolytic and thrombolytic activity of the preparation is comparable with that of trypsin ...
[Preparation of proteolytic enzymes from the thermophilous actinomycete Actinomyces thermovulgaris str. T-54]. A preparation with a high proteolytic activity has been obtained by acetone precipitation from the culture liquid filtrate of the thermophilous actinomycete Actinomyces thermovulgaris str. T-54. The proteolyti...
1,739
pubmed23n0001_883
[Regulation of glutamine synthetase of the fodder yeast Candida tropicalis by ammonium ions].
The effect of ammonium on glutamine synthetase of fodder yeast Candida tropicalis was studied. Ammonium ions were found to repress the synthesis of glutamine synthetase of fodder yeast and to inhibit the enzyme in the cells. The substitution of glutamic acid for ammonium in the nutrient medium brought about depression ...
[Regulation of glutamine synthetase of the fodder yeast Candida tropicalis by ammonium ions]. The effect of ammonium on glutamine synthetase of fodder yeast Candida tropicalis was studied. Ammonium ions were found to repress the synthesis of glutamine synthetase of fodder yeast and to inhibit the enzyme in the cells. T...
1,735
pubmed23n0001_884
[Rhizopus microsporus strain UzLT-I--a thermotolerant producer of lipase].
The properties of the thermotolerant fungus Rhizopus microsporus strain UzLT-1--producer of lipolyptic enzymes are described. Optimal cultivation conditions--40 degrees, C, pH 4.5--Are determined. The lipolytic activity of the culture on the medium consisting of corn extract (2%), cotton-seed oil (1%) and water is 850 ...
[Rhizopus microsporus strain UzLT-I--a thermotolerant producer of lipase]. The properties of the thermotolerant fungus Rhizopus microsporus strain UzLT-1--producer of lipolyptic enzymes are described. Optimal cultivation conditions--40 degrees, C, pH 4.5--Are determined. The lipolytic activity of the culture on the med...
1,738
pubmed23n0001_885
[Effect of the composition of the nutrient medium on the synthesis of acid-fast alpha-amylase by different strains of Aspergillus].
The capacity of 86 strains of the Aspergillus fungus to synthesize acid stable alpha-amylase was examined. The strains of Asp. niger showing a high capacity of synthesizing the enzyme were isolated. Repeated cultivation of the selected cultures on the Minoda agar medium led to a 200% increase in the enzyme activity in ...
[Effect of the composition of the nutrient medium on the synthesis of acid-fast alpha-amylase by different strains of Aspergillus]. The capacity of 86 strains of the Aspergillus fungus to synthesize acid stable alpha-amylase was examined. The strains of Asp. niger showing a high capacity of synthesizing the enzyme were...
1,736
pubmed23n0001_886
[Analysis of acid proteinases from Aspergillus terricola].
The specific action and composition of the functional groups of active centres of three fractions of acid proteinases from Aspergillus terricola have been studied. With respect to the hydrolysis rates of acetyl-L-phenylalanyl-L-tyrosine and carbobenzoxy-D, L-glycyl-phenylalanine by the three fractions it is suggested t...
[Analysis of acid proteinases from Aspergillus terricola]. The specific action and composition of the functional groups of active centres of three fractions of acid proteinases from Aspergillus terricola have been studied. With respect to the hydrolysis rates of acetyl-L-phenylalanyl-L-tyrosine and carbobenzoxy-D, L-gl...
1,740
pubmed23n0001_887
[Thermo- and pH-stability of soluble and silichrome-80 immobilized proteinase from Bacillus subtilis].
The stability to heating and pH changing of two proteinases of Bacillus subtilis was studied. The preparations were: protosubtilin G10x with an activity of 21500 units/g and proteinase immobilized on silochrome C-80 with an activity of 3880 units/g. For protosubtilin G10x the pH optimum was 7.0-7.2. For the immobilized...
[Thermo- and pH-stability of soluble and silichrome-80 immobilized proteinase from Bacillus subtilis]. The stability to heating and pH changing of two proteinases of Bacillus subtilis was studied. The preparations were: protosubtilin G10x with an activity of 21500 units/g and proteinase immobilized on silochrome C-80 w...
1,741
pubmed23n0001_888
Regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase.
We have examined three mutants of Klebsiella aerogenes whose genetic lesions (glnB, glnD, and glnE) are in loci unlinked to the structural gene for glutamine sythetase (glnA) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. Each mutation alters a different compon...
Regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase. We have examined three mutants of Klebsiella aerogenes whose genetic lesions (glnB, glnD, and glnE) are in loci unlinked to the structural gene for glutamine sythetase (glnA) and in which the control of both the level and state of ade...
1,744
pubmed23n0001_889
[Preparation and properties of beta-galactosidase linked covalently with KM-cellulose].
Fungal beta-galactosidase was immobilized by covalent binding with KM-cellulose. The resultant preparation contained 3 mg protein per 1 g carrier; its specific activity was 65% of the initial one. As a result of immobilization pH optimum remained unchanged whereas the temperature optimum decreased from 65 degrees to 50...
[Preparation and properties of beta-galactosidase linked covalently with KM-cellulose]. Fungal beta-galactosidase was immobilized by covalent binding with KM-cellulose. The resultant preparation contained 3 mg protein per 1 g carrier; its specific activity was 65% of the initial one. As a result of immobilization pH op...
1,742
pubmed23n0001_890
[Conditions for splitting protodioscine--the main glycoside from Tribulus terrestris L. by the enzymatic preparation from Aspergillus niger BKMt-33].
The conditions for splitting protodioscine--the main steroid saponine isolated from Tribulus terrestris L. by the enzymic preparation of Aspergillus niger str. BKMt-33 were investigated. The optimal conditions were found to be as follows: pH 4-5, temperature 30-37 degrees (the substrate concentration--5 mg%, concentrat...
[Conditions for splitting protodioscine--the main glycoside from Tribulus terrestris L. by the enzymatic preparation from Aspergillus niger BKMt-33]. The conditions for splitting protodioscine--the main steroid saponine isolated from Tribulus terrestris L. by the enzymic preparation of Aspergillus niger str. BKMt-33 we...
1,743
pubmed23n0001_891
Neuronal properties of hybrid neuroblastoma X sympathetic ganglion cells.
Clonal mouse neuroblastoma cells without tyrosine 3-monooxygenase [EC 1.14.16.2; tyrosine hydroxylase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating)] activity were fused with normal cells from embryonic mouse sympathetic ganglia. One of the 37 hybrid cell lines obtained possesses high tyrosine...
Neuronal properties of hybrid neuroblastoma X sympathetic ganglion cells. Clonal mouse neuroblastoma cells without tyrosine 3-monooxygenase [EC 1.14.16.2; tyrosine hydroxylase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating)] activity were fused with normal cells from embryonic mouse sympathetic...
1,745
pubmed23n0001_892
Regulation of ferredoxin-catalyzed photosynthetic phosphorylations.
Under aerobic conditions that are likely to prevail in chloroplasts in vivo, the optimal concentration of ferredoxin for cyclic photophosphorylation was found to be equal to that required for NADP reduction and about one-tenth of that needed for cyclic photophosphorylation under anaerobic conditions. In the presence of...
Regulation of ferredoxin-catalyzed photosynthetic phosphorylations. Under aerobic conditions that are likely to prevail in chloroplasts in vivo, the optimal concentration of ferredoxin for cyclic photophosphorylation was found to be equal to that required for NADP reduction and about one-tenth of that needed for cyclic...
1,746
pubmed23n0001_893
High resolution 31P nuclear magnetic resonance studies of intact yeast cells.
High resolution 31P nuclear magnetic resonance (NMR) spectra at 145.7 MHZ are presented for intact yeast cells. Several peaks are resolved and assigned. They include the middle phosphate peaks from long chain or cyclic polyphosphates. Our results are consistent with the suggestion that these polyphosphates act as a pho...
High resolution 31P nuclear magnetic resonance studies of intact yeast cells. High resolution 31P nuclear magnetic resonance (NMR) spectra at 145.7 MHZ are presented for intact yeast cells. Several peaks are resolved and assigned. They include the middle phosphate peaks from long chain or cyclic polyphosphates. Our res...
1,747
pubmed23n0001_894
Reversal of UDP-galactose 4-epimerase deficiency of human leukocytes in culture.
Stimulation with phytohemagglutinin of the leukocytes from six of the seven known individuals with UDP-galactose 4-epimerase (= UDP-glucose 4-epimerase; EC 5.1.3.2) deficiency consistently resulted in the appearance of epimerase activity in the cultured cells. A long-term lymphoblast culture derived from one proband al...
Reversal of UDP-galactose 4-epimerase deficiency of human leukocytes in culture. Stimulation with phytohemagglutinin of the leukocytes from six of the seven known individuals with UDP-galactose 4-epimerase (= UDP-glucose 4-epimerase; EC 5.1.3.2) deficiency consistently resulted in the appearance of epimerase activity i...
1,748
pubmed23n0001_895
beta-Bungarotoxin, a pre-synaptic toxin with enzymatic activity.
beta-Bungarotoxin, a pre-synaptic neurotoxin isolated from the venom of the snake Bungarus multicinctus, has been shown to modify release of neurotransmitter at the neuromuscular junction. In this communication, we demonstrate that beta-bungarotoxin is a potent phospholipase A2 (phosphatide 2-acyl hydrolase, EC 3.1.1.4...
beta-Bungarotoxin, a pre-synaptic toxin with enzymatic activity. beta-Bungarotoxin, a pre-synaptic neurotoxin isolated from the venom of the snake Bungarus multicinctus, has been shown to modify release of neurotransmitter at the neuromuscular junction. In this communication, we demonstrate that beta-bungarotoxin is a ...
1,749
pubmed23n0001_896
Isolation and characterization of an unsaturated fatty acid-requiring mutant of cultured mammalian cells.
An unsaturated fatty acid-requiring mutant derived from Chinese hamster ovary (CHO) cells has been isolated and characterized. This mutant grows normally when oleate or other unsaturated fatty acids are supplemented in the growth medium. Unlike the wild-type CHO cells, growth stops when medium is deprived of unsaturate...
Isolation and characterization of an unsaturated fatty acid-requiring mutant of cultured mammalian cells. An unsaturated fatty acid-requiring mutant derived from Chinese hamster ovary (CHO) cells has been isolated and characterized. This mutant grows normally when oleate or other unsaturated fatty acids are supplemente...
1,750
pubmed23n0001_897
DNA single-strand breaks during repair of UV damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum.
The method of DNA alkaline elution was applied to a study of the formation and resealing of DNA single-strand breaks after irradiation of human fibroblasts with ultraviolet light (UV). The general features of the results were consistent with current concepts of DNA excision repair, in that breaks appeared rapidly after...
DNA single-strand breaks during repair of UV damage in human fibroblasts and abnormalities of repair in xeroderma pigmentosum. The method of DNA alkaline elution was applied to a study of the formation and resealing of DNA single-strand breaks after irradiation of human fibroblasts with ultraviolet light (UV). The gene...
1,751
pubmed23n0001_898
Modifications of purified glucose-6-phosphate dehydrogenase and other enzymes by a factor of low molecular weight abundant in some leukemic cells.
Highly purified platelet glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) can be modified in its isoelectric point and its molecular specific activity by extracts of some leukemic granulocytes. The "G6PD modifying factors" are relatively small molecules (molecular weig...
Modifications of purified glucose-6-phosphate dehydrogenase and other enzymes by a factor of low molecular weight abundant in some leukemic cells. Highly purified platelet glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) can be modified in its isoelectric point and its...
1,752
pubmed23n0001_899
Copper-induced activation of aortic lysyl oxidase in vivo.
Raising day-old chicks on diets lacking copper severely depressed the activity of lysyl oxidase, a copper metalloenzyme in connective tissue. Administration of CuSO4 either through the diet or through intraperitoneal injections restored the lysyl oxidase activity in aortic tissue. Two hours after the chicks received Cu...
Copper-induced activation of aortic lysyl oxidase in vivo. Raising day-old chicks on diets lacking copper severely depressed the activity of lysyl oxidase, a copper metalloenzyme in connective tissue. Administration of CuSO4 either through the diet or through intraperitoneal injections restored the lysyl oxidase activi...
1,753