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id stringlengths 15 19	title stringlengths 4 685	content stringlengths 54 6.04k	contents stringlengths 77 6.1k	PMID int64 21 3.77M
pubmed23n0001_600	The renaturation of reduced polyalanyl-chymotrypsinogen and chymotrypsinogen.	Chymotrypsinogen has been successfully renatured in solution, after reduction of its 5 disulfide bonds in 6 M guanidine-HCl. This has been made possible by the study of the renaturation of a model derivative, polyalanyl-chymotrypsinogen. The reduced derivative is shown to refold and reoxodize spontaneously, with a 30-4...	The renaturation of reduced polyalanyl-chymotrypsinogen and chymotrypsinogen. Chymotrypsinogen has been successfully renatured in solution, after reduction of its 5 disulfide bonds in 6 M guanidine-HCl. This has been made possible by the study of the renaturation of a model derivative, polyalanyl-chymotrypsinogen. The ...	1,254
pubmed23n0001_601	Further studies on lipid-peroxide formation in isolated hepatocytes.	Lipid peroxide formation was initiated by the addition of either ADP-complexed Fe3+ or cumene hydroperoxide to a suspension of isolated hepatocytes. The reaction was monitored by malonaldehyde measurements. Upon the addition of iron, malonaldehyde production in the cells started immediately but ceased within 30-60 min,...	Further studies on lipid-peroxide formation in isolated hepatocytes. Lipid peroxide formation was initiated by the addition of either ADP-complexed Fe3+ or cumene hydroperoxide to a suspension of isolated hepatocytes. The reaction was monitored by malonaldehyde measurements. Upon the addition of iron, malonaldehyde pro...	1,255
pubmed23n0001_602	On the mechanism of ketogenesis and its control. Purification, kinetic mechanism and regulation of different forms of mitochondrial acetoacetyl-CoA thiolases from ox liver.	1. Two mitochondrial forms of acetoacetyl-CoA thiolases designated as enzyme A and enzyme B were crystallized from ox liver. They could be shown to be homogenous by polyacrylamide gel electrophoresis. 2. In direction of acetoacetyl-CoA cleavage enzyme A shows a double competitive substrate inhibition when acetoacetyl-C...	On the mechanism of ketogenesis and its control. Purification, kinetic mechanism and regulation of different forms of mitochondrial acetoacetyl-CoA thiolases from ox liver. 1. Two mitochondrial forms of acetoacetyl-CoA thiolases designated as enzyme A and enzyme B were crystallized from ox liver. They could be shown to...	1,256
pubmed23n0001_603	D-glucose-6-phosphate dehydrogenase (Entner-Doudoroff enzyme) from Pseudomonas fluorescens. Purification, properties and regulation.	1. The existence of two different D-glucose-6-phosphate dehydrogenases in Pseudomonas fluorescens has been demonstrated. Based on their different specificity and their different metabolic regulation one enzyme is appointed to the Entner-Doudoroff pathway and the other to the hexose monophosphate pathway. 2. A procedure...	D-glucose-6-phosphate dehydrogenase (Entner-Doudoroff enzyme) from Pseudomonas fluorescens. Purification, properties and regulation. 1. The existence of two different D-glucose-6-phosphate dehydrogenases in Pseudomonas fluorescens has been demonstrated. Based on their different specificity and their different metabolic...	1,257
pubmed23n0001_604	Purification and properties of potato 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-alpha-(1,4-alpha-glucano)-transferase. Evidence against a dual catalytic function in amylose-branching enzyme.	Q-Enzyme, the enzyme that synthesizes the 1,6-alpha-glucosidic branch linkages of amylopectin, has been purified from potato to near homogeneity. The molecular weight of the enzyme is 85000. The active enzyme is a monomer, with a molar activity at pH 7.0 and 24 degrees C of 15. The energy of activation is 25 kJ/mol bel...	Purification and properties of potato 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-alpha-(1,4-alpha-glucano)-transferase. Evidence against a dual catalytic function in amylose-branching enzyme. Q-Enzyme, the enzyme that synthesizes the 1,6-alpha-glucosidic branch linkages of amylopectin, has been purified from potato to nea...	1,258
pubmed23n0001_605	Factors affecting the molecular structure and the agglutinating ability of concanavalin A and other lectins.	Ultracentrifugation analyses were performed on lectins under varying conditions of pH, ionic strength and temperature. It has been demonstrated that the phytohemagglutinin from Phaseolus vulgaris, the wheat germ agglutinin and the soybean agglutinin are stable when these parameters are varied, whereas the concanavalin ...	Factors affecting the molecular structure and the agglutinating ability of concanavalin A and other lectins. Ultracentrifugation analyses were performed on lectins under varying conditions of pH, ionic strength and temperature. It has been demonstrated that the phytohemagglutinin from Phaseolus vulgaris, the wheat germ...	1,259
pubmed23n0001_606	Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins.	The chemical composition of axoplasm extracted from the giant axon of Myxicola infundibulum has been analysed, and some of the factors which disperse its gel structure have been identified. 2. The axoplasm contains about 3-6% protein, and 0-12% lipid. It is isosmotic with sea water and has a pH near 7-0. 3. Inorganic i...	Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins. The chemical composition of axoplasm extracted from the giant axon of Myxicola infundibulum has been analysed, and some of the factors which disperse its gel structure have been identified. 2. The axoplasm contains about 3-6...	1,260
pubmed23n0001_607	The metabolism of cyclohexanol by Acinetobacter NCIB 9871.	Acinetobacter NCIB 9871 was isolated by elective culture on cyclohexanol and grows with this compound as sole source of carbon. It displays a restricted growth spectrum, being unable to grow on a wide range of alternative alicyclic alcohols and ketones. Cyclohexanol-grown cells oxidize the growth substrate at a rate of...	The metabolism of cyclohexanol by Acinetobacter NCIB 9871. Acinetobacter NCIB 9871 was isolated by elective culture on cyclohexanol and grows with this compound as sole source of carbon. It displays a restricted growth spectrum, being unable to grow on a wide range of alternative alicyclic alcohols and ketones. Cyclohe...	1,261
pubmed23n0001_608	Multiple forms of human glutathione S-transferase and their affinity for bilirubin.	The initial enzymic step in mercapturic acid formation is catalyzed by glutathione S-transferase. Several species of this enzyme, designated as transferases alpha, beta, gamma, delta and epsilon on the basis of increasing isoelectric points, were isolated from human liver. Evidence is presented that each of the purifie...	Multiple forms of human glutathione S-transferase and their affinity for bilirubin. The initial enzymic step in mercapturic acid formation is catalyzed by glutathione S-transferase. Several species of this enzyme, designated as transferases alpha, beta, gamma, delta and epsilon on the basis of increasing isoelectric po...	1,262
pubmed23n0001_609	Hydrophobic interaction determined by partition in aqueous two-phase systems. Partition of proteins in systems containing fatty-acid esters of poly(ethylene glycol).	In this report we describe a new method which is useful for measuring hydrophobic interactions between aliphatic hydrocarbon chains and proteins in aqueous environment. The method is based on partition of proteins in an aqueous two-phase system containing dextran and poly(ethylene glycol) and different fatty acid ester...	Hydrophobic interaction determined by partition in aqueous two-phase systems. Partition of proteins in systems containing fatty-acid esters of poly(ethylene glycol). In this report we describe a new method which is useful for measuring hydrophobic interactions between aliphatic hydrocarbon chains and proteins in aqueou...	1,263
pubmed23n0001_610	Spin-labelled phosphofructokinase. A simple and direct approach to the study of allosteric equilibria under near-physiological conditions.	Rabbit muscle phosphofructokinase, spin-labelled at its most reactive thiol group, has an electron spin resonance spectrum which is very sensitive to the binding of substrates and allosteric effectors. The spectral changes have been interpreted in terms of a concerted allosteric transition between two conformational st...	Spin-labelled phosphofructokinase. A simple and direct approach to the study of allosteric equilibria under near-physiological conditions. Rabbit muscle phosphofructokinase, spin-labelled at its most reactive thiol group, has an electron spin resonance spectrum which is very sensitive to the binding of substrates and a...	1,264
pubmed23n0001_611	Cytochrome c interaction with membranes. Absorption and emission spectra and binding characteristics of iron-free cytochrome c.	A cytochrome c derivative from which iron is removed has been prepared and characterized. Several lines of evidence indicate that native and porphyrin cytochrome c have similar conformations: they have similar elution characteristics on Sephadex gel chromatography; in both proteins the tryptophan fluorescence is quench...	Cytochrome c interaction with membranes. Absorption and emission spectra and binding characteristics of iron-free cytochrome c. A cytochrome c derivative from which iron is removed has been prepared and characterized. Several lines of evidence indicate that native and porphyrin cytochrome c have similar conformations: ...	1,265
pubmed23n0001_612	The trypsin and chymotrypsin inhibitors in chick peas (Cicer arietinum L.). Purification and properties of the inhibitors.	From a crude extract of chick peas (Cicer arietinum L.) inhibitors of trypsin and chymotrypsin were isolated by affinity chromatography on a column of trypsin-Sepharose 6B. The content of inhibitors was found to be 1.5 g/kg. They were further separated into six isoinhibitors by ion-exchange chromatography on DEAE-Sepha...	The trypsin and chymotrypsin inhibitors in chick peas (Cicer arietinum L.). Purification and properties of the inhibitors. From a crude extract of chick peas (Cicer arietinum L.) inhibitors of trypsin and chymotrypsin were isolated by affinity chromatography on a column of trypsin-Sepharose 6B. The content of inhibitor...	1,266
pubmed23n0001_613	Investigations of the structure of 3-methylcrotonyl-CoA carboxylase from Achromobacter.	It was shown by gel electrophoresis in sodium dodecylsulphate solution that 3-methylcrotonyl-CoA carboxylase from Achromobacter IVS is composed of two different subunits with molecular weights of about 78000 and 96000, respectively. The biotin is bound to the heavier subunit. It was previously found that 3-methylcroton...	Investigations of the structure of 3-methylcrotonyl-CoA carboxylase from Achromobacter. It was shown by gel electrophoresis in sodium dodecylsulphate solution that 3-methylcrotonyl-CoA carboxylase from Achromobacter IVS is composed of two different subunits with molecular weights of about 78000 and 96000, respectively....	1,267
pubmed23n0001_614	Polyadenylated RNA from Vicia faba meristematic root cells. Localization and size estimation of the poly (A) segment.	After incubating root apices from two-day-old bean seedlings with [3H] adenine the RNA was extracted from whole cells or polysomes, and the poly (A) sequences were isolated by nuclease digestion followed by poly(U)-Sepharose chromatography. The alterations of the RNA molecules due to the various treatments were monitor...	Polyadenylated RNA from Vicia faba meristematic root cells. Localization and size estimation of the poly (A) segment. After incubating root apices from two-day-old bean seedlings with [3H] adenine the RNA was extracted from whole cells or polysomes, and the poly (A) sequences were isolated by nuclease digestion followe...	1,268
pubmed23n0001_615	Time-dependent inhibition of diamine oxidase by carbonyl-group reagents and urea.	1. The behaviour of several carbonyl group reagents and urea as time-dependent inhibitors of both pig kidney and human placental diamine oxidase is described. 2. Plots of log (vt/vo) against time were not linear with these reagents as the usual theories predict. 3. This was particularly the case with aminoguanidine and...	Time-dependent inhibition of diamine oxidase by carbonyl-group reagents and urea. 1. The behaviour of several carbonyl group reagents and urea as time-dependent inhibitors of both pig kidney and human placental diamine oxidase is described. 2. Plots of log (vt/vo) against time were not linear with these reagents as the...	1,269
pubmed23n0001_616	Acetylglucagon: preparation and characterization.	Acetylated derivatives of glucagon have been prepared by reacting this hormone under various conditions with acetic anhydride. They have been chemically characterized by the use of a 14C-labeled reagent, by peptide mapping techniques following hydrolysis by pronase and chymotrypsin, and by spectroscopy. Acetylation in ...	Acetylglucagon: preparation and characterization. Acetylated derivatives of glucagon have been prepared by reacting this hormone under various conditions with acetic anhydride. They have been chemically characterized by the use of a 14C-labeled reagent, by peptide mapping techniques following hydrolysis by pronase and ...	1,270
pubmed23n0001_617	Purification and properties of a periplasmic aminoendopeptidase from Escherichia coli.	A periplasmic aminoendopeptidase from Escherichia coli has been purified to hemogeneity. It is a monomer of molecular weight 45000 and containing one -- SH group that is necessary for catalytic activity. The study of its substrate specificity indicated that the enzyme has both aminopeptidase and endopeptidase activity....	Purification and properties of a periplasmic aminoendopeptidase from Escherichia coli. A periplasmic aminoendopeptidase from Escherichia coli has been purified to hemogeneity. It is a monomer of molecular weight 45000 and containing one -- SH group that is necessary for catalytic activity. The study of its substrate sp...	1,271
pubmed23n0001_618	The interaction of organic phosphates with human and chicken hemoglobin.	In this study of the binding properties of inositol hexaphosphate and 2,3-bisphosphoglycerate to chicken and human deoxyhemoglobin and carboxyhemoglobin were compared. It appeared that in all cases the binding to chicken hemoglobin is much stronger than to human hemoglobin. This is very probably due to the fact that 4 ...	The interaction of organic phosphates with human and chicken hemoglobin. In this study of the binding properties of inositol hexaphosphate and 2,3-bisphosphoglycerate to chicken and human deoxyhemoglobin and carboxyhemoglobin were compared. It appeared that in all cases the binding to chicken hemoglobin is much stronge...	1,272
pubmed23n0001_619	Hepatic nucleases. Extrahepatic origin and association of neutral liver ribonuclease with lysosomes.	In the large granule fraction of rat liver, the density distribution of inhibitor-sensitive neutral ribonuclease is similar to that for acid hydrolases and its density distribution is similarly modified by Triton WR-1339 accumulation in lysosomes. Particulate neutral ribonuclease is latent; the enzyme is unmasked by ve...	Hepatic nucleases. Extrahepatic origin and association of neutral liver ribonuclease with lysosomes. In the large granule fraction of rat liver, the density distribution of inhibitor-sensitive neutral ribonuclease is similar to that for acid hydrolases and its density distribution is similarly modified by Triton WR-133...	1,273
pubmed23n0001_620	Glutathione reductase from human erythrocytes. Molecular weight, subunit composition and aggregation properties.	Glutathione reductase from human erythrocytes exists predominatly as an entity of 100 000 molecular weight under various conditions of pH and ionic strength. The S20,W of 5.5 S and D20W of 50 mum2/s correlate with the molecular weight determined by sedimentation equilibrium. The homogeneity of this species is primarily...	Glutathione reductase from human erythrocytes. Molecular weight, subunit composition and aggregation properties. Glutathione reductase from human erythrocytes exists predominatly as an entity of 100 000 molecular weight under various conditions of pH and ionic strength. The S20,W of 5.5 S and D20W of 50 mum2/s correlat...	1,274
pubmed23n0001_621	GAMMA-Glutamyl transpeptidase of sheep-kidney cortex. Isolation, catalytic properties and dissociation into two polypeptide chains.	Gamma-Glutamyl transpeptidase was isolated from sheep kidney cortex as an apparently homogeneous, highly active protein. At optimal pH and in the absence of acceptors, the enzyme catalyzes the release of about 510 mumol of p-nitroaniline per mg protein per min from the model substrate L-gamma-glutamyl-p-nitroanilide. P...	GAMMA-Glutamyl transpeptidase of sheep-kidney cortex. Isolation, catalytic properties and dissociation into two polypeptide chains. Gamma-Glutamyl transpeptidase was isolated from sheep kidney cortex as an apparently homogeneous, highly active protein. At optimal pH and in the absence of acceptors, the enzyme catalyzes...	1,275
pubmed23n0001_622	Pyrophosphatase and glucuronosyltransferase in microsomal UDPglucuronic-acid metabolism in the rat liver.	1. A radiochemical method for the studies on the microsomal UDPglucuronic acid metabolism has been developed. 2. The rat liver microsomes caused a rapid hydrolysis of UDPglucuronic acid to D-glucuronic acid 1-phosphate and further although much slower to free D-glucuronic acid. In Tris-HCl buffer (pH 7.4) they were pro...	Pyrophosphatase and glucuronosyltransferase in microsomal UDPglucuronic-acid metabolism in the rat liver. 1. A radiochemical method for the studies on the microsomal UDPglucuronic acid metabolism has been developed. 2. The rat liver microsomes caused a rapid hydrolysis of UDPglucuronic acid to D-glucuronic acid 1-phosp...	1,276
pubmed23n0001_623	Incorporation of (1-14C)palmitoyl-CoA into phosphatidylcholine by plasma membranes of rat submaxillary glands in vitro.	1. On incubation with the isolated rat submaxillary gland plasma membranes, [1-14C]palmitoyl-CoA was incorporated mainly into phosphatidylcholine and hydrolysed to [1-14C]palmitic acid and CoASH. 2. The addition of lysophosphatidylcholine enhanced the incorporation into phosphatidylcholine and lowered the hydrolysis of...	Incorporation of (1-14C)palmitoyl-CoA into phosphatidylcholine by plasma membranes of rat submaxillary glands in vitro. 1. On incubation with the isolated rat submaxillary gland plasma membranes, [1-14C]palmitoyl-CoA was incorporated mainly into phosphatidylcholine and hydrolysed to [1-14C]palmitic acid and CoASH. 2. T...	1,278
pubmed23n0001_624	Hydrogen-isotope exchange of oxidized and reduced cytochrome c. A comparison of mass spectrometry and infrared methods.	Hydrogen-deuterium exchange in 2H20 solutions of the two redox states of horse heart cytochrome c was investigated at 20 degrees C, pH 7, by mass spectrometry and infrared spectroscopy. Mass spectrometry indicates that ferricytochrome has 20 hydrogens unexchanged after 24 h, 28 hydrogens exchanging between 10 min and 2...	Hydrogen-isotope exchange of oxidized and reduced cytochrome c. A comparison of mass spectrometry and infrared methods. Hydrogen-deuterium exchange in 2H20 solutions of the two redox states of horse heart cytochrome c was investigated at 20 degrees C, pH 7, by mass spectrometry and infrared spectroscopy. Mass spectrome...	1,279
pubmed23n0001_625	Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC.	The hydroxylase activities observed in extracts of Pseudomonas putida ORC after growth on orcinol and resorcinol as sole source of carbon have been purified to homogeneity. Both enzymes were shown to be flavoproteins and to contain approximately 1 mol of FAD for each polypeptide chain, S20,W values for each enzyme are ...	Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC. The hydroxylase activities observed in extracts of Pseudomonas putida ORC after growth on orcinol and resorcinol as sole source of carbon have been purified to homog...	1,280
pubmed23n0001_626	[Possible structural-functional organization of the system of local cerebral blood flow regulation].	rCBF under normal conditions in the rabbit, cat, and monkey brain was found to have a spontaneous periodicity while rCBF responses to afferent flicker stimulation usually revealed a double-phasic fluctuative pattern. This suggests that the rCBF regulatory system consists of not less than two regulatory chains with diff...	[Possible structural-functional organization of the system of local cerebral blood flow regulation]. rCBF under normal conditions in the rabbit, cat, and monkey brain was found to have a spontaneous periodicity while rCBF responses to afferent flicker stimulation usually revealed a double-phasic fluctuative pattern. Th...	1,291
pubmed23n0001_627	[Regulation of local tissue PO2 in the cerebral cortex of the cat].	Local PO2 was measured in the cat cortex on adjacent sites with a platinum multiwire surface electrode both during steady state conditions and with varying arterial oxygen supply. Concomitantly, PO2 in the sinus sagittalis was recorded continuously through the vascular wall. Under normoxia and steady state conditions l...	[Regulation of local tissue PO2 in the cerebral cortex of the cat]. Local PO2 was measured in the cat cortex on adjacent sites with a platinum multiwire surface electrode both during steady state conditions and with varying arterial oxygen supply. Concomitantly, PO2 in the sinus sagittalis was recorded continuously thr...	1,292
pubmed23n0001_628	[Differences in the responses of taste receptors to organic and inorganic acids with changes in the concentration of bicarbonate in the solution].	The thresholds of the pH for citric acid (pH=4.9) were found to exceed by 1.4 pH the thresholds for HC1 (3.5) at 1.2 mmol/1 bicarbonate in the solution. The reaction to citric acid was higher than to HC1 at equal pH. Decreasing of bicarbonate from 1.2 mmol/1 to 0 reduced pH threshold only for sitric acid from 4.90 to 3...	[Differences in the responses of taste receptors to organic and inorganic acids with changes in the concentration of bicarbonate in the solution]. The thresholds of the pH for citric acid (pH=4.9) were found to exceed by 1.4 pH the thresholds for HC1 (3.5) at 1.2 mmol/1 bicarbonate in the solution. The reaction to citr...	1,293
pubmed23n0001_629	Analysis of skin grafts across the MSA-barrier in mice pretreated with sera from specifically or syngeeically grafted donors.	Prolonged survival of weakly incompatible skin allografts in mice (across the barrier presented by the MSA) can be induced by pretreating the recipients not only with a specific anti-MSA serum (obtained on day 5 after a single MSA-incompatible skin graft) but also be means of control serum obtained in a similar way fro...	Analysis of skin grafts across the MSA-barrier in mice pretreated with sera from specifically or syngeeically grafted donors. Prolonged survival of weakly incompatible skin allografts in mice (across the barrier presented by the MSA) can be induced by pretreating the recipients not only with a specific anti-MSA serum (...	1,295
pubmed23n0001_630	Effect of normal and ulcer-type diet on the acidity of gastric contents in patients with duodenal ulcer.	Patients with uncomplicated duodenal ulcer were given two types of diet -a normal and a ulcer-type diet. The data obtained did not show any statistically significant difference between the action of the two diets. No evidence was then found to be in support of the still widely used restricted diet in the treatment of p...	Effect of normal and ulcer-type diet on the acidity of gastric contents in patients with duodenal ulcer. Patients with uncomplicated duodenal ulcer were given two types of diet -a normal and a ulcer-type diet. The data obtained did not show any statistically significant difference between the action of the two diets. N...	1,298
pubmed23n0001_631	Impaired Sertoli cell function in experimental cryptorchidism in the rat.	The production of testicular androgen-binding protein (ABP), as a measure of Sertoli cell function, was studied after unilateral or bilateral experimental cryptorchidism in adult rats. Two or 4 weeks after the testis had been translocated to the abdomen, no major changes were found in the concentration of ABP per mg pr...	Impaired Sertoli cell function in experimental cryptorchidism in the rat. The production of testicular androgen-binding protein (ABP), as a measure of Sertoli cell function, was studied after unilateral or bilateral experimental cryptorchidism in adult rats. Two or 4 weeks after the testis had been translocated to the ...	1,296
pubmed23n0001_632	The oxygen transport system of red blood cells during diabetic ketoacidosis and recovery.	Daily evaluations of 8 newly detected ketoacidotic diabetics showed the Bohr-effect of haemoglobin to be decreased by 50% while erythrocyte 2,3-DPG was decreased below 10 mumoles/g Hb. 2,3-DPG correlated strongly with pH during acidosis and with plasma inorganic phosphate (Pi) subsequently to the first insulin administ...	The oxygen transport system of red blood cells during diabetic ketoacidosis and recovery. Daily evaluations of 8 newly detected ketoacidotic diabetics showed the Bohr-effect of haemoglobin to be decreased by 50% while erythrocyte 2,3-DPG was decreased below 10 mumoles/g Hb. 2,3-DPG correlated strongly with pH during ac...	1,319
pubmed23n0001_633	Fluorescence of oxidized flavoproteins from perifused isolated pancreatic islets.	In perifused pancreatic islets, the fluorescence of oxidized flavoproteins (FAD) was recorded continuously. Elevation of glucose concentration in the medium form 0 or 5 mM to 20 mM led to decrease in FAD-fluorescence beginning 10 sec after change of medium. L-leucine (10 mM), (+/-)-B-BCH (20 mM) and alpha-ketoisocaproi...	Fluorescence of oxidized flavoproteins from perifused isolated pancreatic islets. In perifused pancreatic islets, the fluorescence of oxidized flavoproteins (FAD) was recorded continuously. Elevation of glucose concentration in the medium form 0 or 5 mM to 20 mM led to decrease in FAD-fluorescence beginning 10 sec afte...	1,320
pubmed23n0001_634	Comparison of amino acids bathing the oxyntic gland area in the stimulation of gastric secretion.	This study was undertaken to compare the ability of L- and D-isomers of amino acids bathing the oxyntic gland area to stimulate acid secretion in conscious dogs with Heidenhain pouch (HP), gastric fistula (GF) and pancreatic fistula (PF). Acid outputs from HP were determined by an intragastric titration method when ami...	Comparison of amino acids bathing the oxyntic gland area in the stimulation of gastric secretion. This study was undertaken to compare the ability of L- and D-isomers of amino acids bathing the oxyntic gland area to stimulate acid secretion in conscious dogs with Heidenhain pouch (HP), gastric fistula (GF) and pancreat...	1,322
pubmed23n0001_635	Evidence of tandem duplication of genes in a merodiploid region of Pneumococcal mutants resistant to sulfonamide.	A Pneumococcal mutant, sulr-c, resistant to sulfonamides, and three transformants bearing associated d or d+ resistance markers have earlier been reported to be unstable and show distinct patterns and frequencies of segregating stable progeny lacking the c marker. Each of the four strains showed a characteristic dosage...	Evidence of tandem duplication of genes in a merodiploid region of Pneumococcal mutants resistant to sulfonamide. A Pneumococcal mutant, sulr-c, resistant to sulfonamides, and three transformants bearing associated d or d+ resistance markers have earlier been reported to be unstable and show distinct patterns and frequ...	1,323
pubmed23n0001_636	A study of Pneumococcal merodiploids at the molecular level.	The DNA of a sulfonamide-resistant Pneumococcal strain (heterozygous for sulr-c) and that of three highly resistant and persistently heterozygous cd transformants, derived by introducing sulr-c marker into a stable sulfonamide resistant strain (sulr-d), were studied to analyze the genetic basis of their merodiploidy. T...	A study of Pneumococcal merodiploids at the molecular level. The DNA of a sulfonamide-resistant Pneumococcal strain (heterozygous for sulr-c) and that of three highly resistant and persistently heterozygous cd transformants, derived by introducing sulr-c marker into a stable sulfonamide resistant strain (sulr-d), were ...	1,324
pubmed23n0001_637	Genetic hybridization at the unlinked thy and str loci of Streptococcus.	The sanguis and pneumoniae species of Streptococcus were used as recipients in transformations from str+ to str-r and from thy- to thy+. The str-r mutations in the two species had been previously shown to be allelic. Homology of the thy- mutations in the two species was demonstrated in the similar phenotypic properties...	Genetic hybridization at the unlinked thy and str loci of Streptococcus. The sanguis and pneumoniae species of Streptococcus were used as recipients in transformations from str+ to str-r and from thy- to thy+. The str-r mutations in the two species had been previously shown to be allelic. Homology of the thy- mutations...	1,325
pubmed23n0001_638	Fibrin plate method with reagents purified by affinity chromatography and its use for determination of fibrinolytic and other proteolytic activity in saliva, bile and plasma.	A modification of the fibrin plate method is presented. Plasminogen-free human fibrinogen and plasminogen purified by affinity chromatography have been used. Fibrin plates without and with a constant amount of plasminogen and with agarose as stabilizing medium were used for the estimation of plasmin and plasminogen act...	Fibrin plate method with reagents purified by affinity chromatography and its use for determination of fibrinolytic and other proteolytic activity in saliva, bile and plasma. A modification of the fibrin plate method is presented. Plasminogen-free human fibrinogen and plasminogen purified by affinity chromatography hav...	1,332
pubmed23n0001_639	[Thefts without motive of gain as a psychopathologic syndrome (author's transl)].	Thefts without motive of pain have been known since the early 19th century. But the problem has not been solved. While they were formerly considered a mental disease, today they are not seen as something special. But they still happen. Only a small percentage of common shop-lifting can be called a psycholopathologic sy...	[Thefts without motive of gain as a psychopathologic syndrome (author's transl)]. Thefts without motive of pain have been known since the early 19th century. But the problem has not been solved. While they were formerly considered a mental disease, today they are not seen as something special. But they still happen. On...	1,333