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1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] RNA-- cytidylate DNA--deoxycytidylate [proteome] | WHat's the name for nucleotide for cytosine RNA and DNA? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] comprised of the 4 nucleotides deoxyguanylate, deoxyadenylate, deoxycytidylate thymidylate 5'-pApCpGpT-3' chain polarity and only one direction. [proteome] | Describe the composition of DNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] single strand 4.6 mil nucleotides long [proteome] | E coli DNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 2 oppositely oriented helical polynucleotide chains coiled around common axis bases are inside, sugars are outside bases perpindicular to axis rise of 3.4 A structures repeat every 34 A so 10 bases / turn and rotation of 36 degrees / base diameter 20A [proteome] | Describe a double helix | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] G=C A=T held together by weak H bonds 1-5 kcal/mol van der waals forces = 0.5-2.0 kcal / mol bases are hydrophobic and clustered inside while polar sugars (- charge) are exposed to water [proteome] | How are bases paired (DNA)? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] basically shows that the double helix splits open and replicates. In the daughter generation one strand on new helix is from parent and one is nacent [proteome] | What is semiconservative replication? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] E Coli parent DNA labeled with 15N. Then they grew Ecoli in 14N environment. Showed the incorporation of 14N during replication They could detect the difference due to the density difference (1.66 vs. 1.76 g/mol) after one replication, density was half for 14N and half for 15N after 2 gen... | Explain the Meselson and Stahl experiment | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] DNA helix can be reversibly melted heat to Tm (temp at which 50% of structure is lost)~70 degrees C or add alkali or acid to ionize bases [proteome] | What is DNA melting? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] a way to determine if bases are stacked or not Stacked bases absorb less light than unstacked so at Tm single strands will abosorb more uv light at 260 nm [proteome] | What is hypochromism? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] continuous may be supercoiled to superhelix for compactibility may be relaxed [proteome] | What are some characteristics of circular DNA? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] made by ssDNA or ssRNA may form watson-crick base pairs with other mismatched base pairs can have non-standard base pairing [proteome] | Describe a stem loop structure | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Kornberg discovered it catalyzes DNA synthesis adds deoxyribonucleotides to preexisting DNA template directed by the existing DNA template some remove mismatched pairings [proteome] | What is DNA polymerase? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] DNA n dNTP (of all 4 bases) 5' deoxynucleoside triphosphates Mg2+ free 3'OH primer [proteome] | What does DNA replication require to take place? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] goes in a 5' -----> 3' direction 3' OH mounts a Nucleophilic attack on the alpha triphosphate chain attached to the 5' of the previous nucleotide PPi (pyrophosphate) is given off [proteome] | How does the template direct DNA replication? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] DNA transcription to mRNA mRNA takes it to ribosome RNA translates with help from tRNA the codons into proteins [proteome] | describe the order in which a protein is made. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] template with codons which serves as the master for translation. 1.2 kb long average for E coli 5% of total RNA (ribosomal RNA, tRNA make up rest) [proteome] | Describe mRNA (E.coli) | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] one for each of 20 amino acids ~75 nucleotides ~25 kDa carries the activated amino acid and with its corresponding anticodon aids in translation in ribosome makes up about 15% of RNA [proteome] | Describe tRNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] ribosomal RNA makes up 80% in E. coli This 80% is comprised of 23S, 16S and 5S species [proteome] | Describe rRNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] only in Eukaryotes works in splicing out the inons [proteome] | Describe snRNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] micro RNA class of small non-coding RNA's (~21 nucleotides These guys bind complementary mRNAs and therefore inhibit translation [proteome] | Describe miRNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] siRNA small non-coding RNA bind up mRNA and degrade it [proteome] | describe small interfering RNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] catalyzes transcription uses dsDNA or ssDNA as template makes and elongates RNA chains [proteome] | Describe RNA polymerases | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] ATP, GTP, UTP, CTP Mg2+ or Mn2+ template but NO primer needed [proteome] | What does an RNA polymerase need to start elongation? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] RNA polymerase aids for the free 3'OH to mount nucleophilic attack on the incoming alpha phosphate reaction is aided by hydrolysis of PPi No proofreading here. The new mRNA strand is identical to the coding DNA strand. (not identical to template, but complementary to template) [proteome] | Describe transcription | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] There is a 5' cap and a 3' poly AAAAAAAA structure added after transcription [proteome] | How do mRNA in Eukaryotes differ from those in Prokaryotes? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] brings amino acids to ribosome for making proteins aminoacyl tRNA synthetase uses ATP to make aminoacyl-tRNA which is a unique tRNA for each amino acid each one of these aminoacyl-tRNA's have a unique anticoding site which will recognize the codon on mRNA [proteome] | Describe the role and mechanism of tRNA | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] amino acids can have more than one codon. 64 possible codons for 20 amino acids 3 triplets are stop codons so the leftover 61 code for the 20 amino acids degenerate code decreases harmful mutations and having too many chain terminators [proteome] | what is and what is the significance of a degenerate genetic code? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 3' end of tRNA attaches to the carboxyl carbon of the amino acid 5' end is phosphorolated [proteome] | Where is the amino acid attached to tRNA? What's on the other end | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Trp Met [proteome] | Which two amino acids only have one codon? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Leu Ser Arg [proteome] | Which three amino acids have 6 codons? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] UAA UAG UGA [proteome] | What are the stop codons? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] UGG Take a Trp to australia to get some uggs [proteome] | What is the codon for Trp | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] AUG Mett's birthday is in AUG [proteome] | What is the codon for Met? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] C and U [proteome] | XY___ and XY____ ALWAYS encode for the same amino acid | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] G and A [proteome] | XY____ and XY_____ USUALLY code for same amino acid | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] On the mRNA chain a 5' shine dalgano (purine rich) marker occurs at -10, and then an AUG or GUG is +1 which tells the fMet to bind there and begin [proteome] | For E. coli what starts translation? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] At the 5' cap, Met-tRNA binds to the first AUG it sees and goes from there. [proteome] | For eukaryotes what starts translation? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] UAA, UAG and UGA are stop codons This binds protein which is not tRNA and it uses release factors to release the new protein [proteome] | How does translation know when to stop? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Nearly universal but some deviations in humans UGA is a code for Trp and not a stop codon instead we have AGA and AGG as stop codons and we don't code for Arg mitochondrial DNA is different because they make their own tRNA's 16 organisms deviate from the standard genetic code [proteome] | Describe how universal the genetic code is. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] introns are unused code and exons are expressed genes which means we must splice these guys. Humans have 8 introns which can be 50-10000 nucleotides long [proteome] | what are introns and exons | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 3 exons and 2 introns [proteome] | Describe human hemoglobin B chain with regard to introns and exons | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] pre m RNA is heavier--has a 15S rate as opposed to the actual mRNA which is 9S rate. [proteome] | Describe how we know about introns and exons? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] assemblies of proteins and small RNA molecules recognizes that introns begin with GU and end with AG preceeded by essential pyrimidine tract [proteome] | What is a spliceosome? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] shuffling allows for recombined genes which may provide diversity of function alternative splicing may lead to new proteins being formed [proteome] | What would be the benefit of exon shuffling or alternative splicing? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] restriction endonucleases cut DNA in certain spots where they recognize specific sequences and cut both strands [proteome] | How do restriction enzymes provide the bases for gene exploration? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] the cleavage site is 4-8 bp at a palindromic symmetry site. They see this symmetry and hydrolyze the phosphodiester bonds here can leave staggered (sticky) or even (blunt) cuts. [proteome] | Where do restriction enzymes know where to cut? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 5' G*AATTC 3' 3' CTTAA*G 3' derived from E. coli [proteome] | EcoRI | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] In cells, these guys cleave foriegn DNA without harming host DNA In labs, we use it to cleave DNA into fingerprints so we can work with it better [proteome] | What are the endogenous and laboratory uses for restriction endonucleases | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 5.1 kb circular DNA has 1 EcoRI cleavage sit has 4 HpaI has 11 HindIII [proteome] | Describe SV40 | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Gel electrophoresis will separate up to 1,000bp (more porous agarose up to 20 kb) Pulse Field Gel Elect. separates chromosomes [proteome] | Once you have DNA fragments what do you do with them to manipulate them? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] stains dsDNA fragments after separated by gel elect. and has sensitivity to show 50ng with orange fluorescence [proteome] | what does ethidium bromide do? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Separate different fragments in gel denature them to make ssDNA use a nitrocellulose sheet a strand, matching the one you are looking for has 32P incorporated. when it finds its ppair it links up and audioradiography will show you which one it is on paper southern blotting [proteome] | Explain how to identify restriction fragments using 32P probe which technique is this? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] mutations on restriction fragment grounds for polymorphism RFLP restriction fragment length polymorphism [proteome] | What other things can southern blots reveal about a restriction fragment? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] same technique as southern but for RNA [proteome] | What is northern blotting? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] The method of incorporating a small amount of dideoxynucleoside triphosphatees which serve to effectively terminate replication at various points primed with synthesized fragment use DNA polymerase add all the deoxyribonucleoside triphosphates and 2' 3' dideoxy gel and audioradiogram to s... | Describe dideoxy method for DNA sequencing | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] fluorescence tagging can run all four bases in one reaction up to 500 bases can be determined automated. can generate more than 1 million bases / day [proteome] | what's a more modern less radioactive sanger method? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] solid phase phosphite triester method use glass bead add excess reagents to drive rxn to completion sequential addition of protonated (proteceted) deoxyribonucleoside 3' phosphoramidites wash soluable protectors away [proteome] | What method is used for making DNA or DNA probes? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] deoxynucleoside 3' phosphoramidite DMT dimethoxytrityl BCE B cyanoethyl I2 (oxidizer) dicholoroacetic acid NH3 [proteome] | what are the reagents for phosphite triester method? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] remove all protecting groups and remove the deoxyribonucleoside 3' phosphoramidite from the glass bead [proteome] | What is the role of NH3 in phosphite triester method? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] removes DMT from 5' end of newly added deoxyribonucleoside. [proteome] | what is role of dichloroacetic acid in phosphite triester method? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] phosphit on 3' of activated monomer attaches to 5 ' OH of chain. [proteome] | where does the triester form in phosphite diester method on activated monomer and chain? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 99% 10 min per cycle elongation is never 100% so the longest one is the golden one. You can find this using PAGE or HPLC [proteome] | What's the efficiency of the phosphite triester method? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] make probes (32P or fluorescence tag and then set it loose in fragments to find the needles in the haystack) use it as a primer for new neighboring DNA make new designer genes which can make new funcitons or new proteins [proteome] | Once you makes some DNA using the phosphite triester method, what can you do with it? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Polymerase Chain Reaction amplifies your sample [proteome] | What is PCR? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] strand separation (heat) hybridize primers (cool with a ton of primers in there --20-30 nucleotides long) heat to 72 so Taq polymerase can start making the strand 5' -3' repeat 25-30 times n cycles = 2tothe n results [proteome] | What are the 3 steps in PCR> | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] DNA fragment + a vector (plasmids or lambda phage) host (E. coli) [proteome] | Describe recombinant DNA technology | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] cuts pSC101 at a specific site leaving a sticky end. If you use Eco RI to cut any piece of inserting DNA those same sticky ends will line up and voila. [proteome] | Describe how Eco RI works with plasmid pSC101 | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] DNA ligase catalyzes the formation of phosphodiester bonds needs a free 3'OH and 5' phosphate as well as ATP or NAD+ [proteome] | What is DNA ligase? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] If you have a DNA segment you can add cohesive ends to put the tape on the ends. polynucleotide kinase phosphorylates the 5' end of a linker T4phage ligase seals them (can be blunt ended). then use EcoRI to make the sticky ends and insert into your vector [proteome] | Describe the cohesive ends (Linkers) method. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] a synthetic DNA fragment programed with several sequences that many restriction endonucleases recognize. Can custom make your sticky ends. [proteome] | what is a polylinker? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] they get the recombinant DNA into bacteria easily which you can then select for. Plasmids carry genes for inactivation of antibiotics and can replicate independently of host genome [proteome] | why are plasmids and lambda phages good vectors? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] has genes for resistance to tetracycline and ampicilin the EcoRI site isn't in these genes for the ab resistance but it does have other restriction enzyme sites which will interrupt the resistance [proteome] | What is it about pBR322 that makes it good for cloning? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] pBR322 uses the enzyme cut in amp. resistant element and foreign DNA ligated in. Now these guys are going to be amp sensitive. Still resistant to tetra. grow E coli on plates of tetra. transferred in matching postions to new plates . One plate has tetra and another has amp and tetra. Now ... | Describe selection using pBR322 | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 2-100+ kb [proteome] | What size is a plasmid? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 48 kb [proteome] | What is the size of lambda phage? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] lytic kills host cell quickly and produces ~100 virons lysogenic inserts DNA into host cell genome and it is replicated for many generations Upon activation it expresses dormant viral DNA [proteome] | What are the differences between a lytic and lysogenic pathway? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] mutant lambda phage with only 2 EcoRI sites as opposed to the normal 5 enter bacteria way easier than plasmid [proteome] | What is lambda gt-lambdaB | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] EcoRI cleavage gives two arms, and foreign DNA inserted to make it 93% (fits in the 75-105% size requirements [proteome] | Describe lambda gt-lambdaB and how it gets packaged. | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] hybrid of lambda phage and a plasmid accomodates large DNA inserts of 45 kb!! [proteome] | what is cosmid lambda? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Bacterial artificial chromosome engineered version of ecoli fertility F factor. accomodates inserts of DNA in 100,000-300,000 bp selectable with chloramphenicol resistance put in bacteria by electroporation [proteome] | What is a BAC | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Yeast Artificial Chromosome accomodates inserts as big as 2,000,000 bp lots of stuff in em that we didn't really cover but that makes them ideal for big genomes [proteome] | What is a YAC | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] mechanically sheared fragments of a 3,000,000 kb genome makes random overlapping fragments which can be PAGEd isolate 15 kb long molecules and add linkers and then insert into lambda phages and then grown in E coli. gives you genomic library [proteome] | What is a genomic digest? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Allows us to screen the genomic library [proteome] | Why do we need DNA hybridization? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] the recombinant phages are plated on a lawn of bacteria where they like guys grow a plaque. Nitrocellulose is then applied and we add NaOH to lyse and denature the DNA. 32P probe DNA is introduced and the target sequence is radioaudiographed. [proteome] | What is DNA hybridization? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] take the mRNA from an enriched source of what you are looking for. Make a cDNA from this mRNA and use as probe use amino acid sequence from target (use sequences with a lot of Trp and Met Synthesize the probes with 32P Use all the probes and isolate and sequence positive clones [proteome]... | How do we know how to make the probe for human hybridization screening? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] lots of peptides are encoded by several oligonucleotides. Sequences where there is alot of Met or Trp are best because they only have one codon [proteome] | Why do we need a probe with minimal degeneracy? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] E. coli doesn't have the ability to splice, so we have to make cDNA from mRNA (which doesn't have introns) [proteome] | Why isn't Ecoli. good for mammalian mRNA? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] Short intersperced elements (DNA that doesn't code for proteins) 1,000,000 Alu sequences of 300bp Long intersperced elements nearly 1 million LINES up to 10 kbp [proteome] | What is the difference between SINES and LINES? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] mRNA + oligo T primer with 3'OH + 4 dNTP's oligo T pairs with poly A at 3' end of mRNA reverse transcriptase makes a DNA complementary to RNA template increase the pH and dissolve mRNA add terminal transferase which adds oligo GGG to 3' end of the ssDNA then add oligo CCCC to make cDNA [p... | how do we make cDNAs | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 125I antibody [proteome] | What antibody do we use in immunochemical screening of cloned cDNA? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] 0.1 mm micropipet yeilds 2% viability foster mother mouse [proteome] | Describe DNA microinjection method/ | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
1a5bd233_r_3_Berg_Flashcards___Cram_com__proteome | [proteins expressed by genome] rat growth hormone in rats it is promoted by metallothionein promoter have an activator [proteome] | what is GH 21-kd protein? | [] | Proteins and Proteomes Chapter 3 Berg Flashcards - Cram.com | proteome | http://www.cram.com/flashcards/proteins-and-proteomes-chapter-3-berg-1808545 | 29/1438042990123.20_20150728002310-00085-ip-10-236-191-2_385146738_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] classical concept | a concept that can be characterized by unchanging criteria: For instance, a triangle can be defined as a three sided figure. | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] color term | any word that refers to a color, e.g. magenta | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] compound word | a word composed of two or more free morphemes (e.g. blackboard, merry-go-round) | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] core group | a small subset of the vocabulary of a child, used very frequently | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] deictic terms | from the Greek deiktikos (able to show); words that are used as linguistic pointers (e.g. here, there) also call deixis. | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] derived word | a complex word made from a base morpheme to which various affixes have been assed: e.g. unhappiness is derived from happy by the addition of the affixes un- and -ness | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] fast mapping | children's ability to form an initial hypothesis about a word's meaning very quickly, after hearing it only once or twice; in depth learning requires multiple exposures to the word in many different contexts, however. | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
30967d05_keyterms_Flashcards___Cram_com____rather_than_animal_or_collie | [basic level category] focal colors | among colors, those that are the most typical, the reddest reds and bluest blues. | [] | 4380 exam 2 ch 4 keyterms Flashcards - Cram.com | the level of abstraction that is most generally appropriate in a given situation or for the given speaker: e.g. dog, rather than animal or collie | http://www.cram.com/flashcards/4380-exam-2-ch-4-keyterms-679875 | 29/1438042987628.47_20150728002307-00057-ip-10-236-191-2_399003266_0.json |
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