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84 20437 ENTREZ_GENE Siah1a AA982064|AI853500|Sinh1a 10090 Mus musculus 0.908 - - - - NO BioGRID ORCS
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84 56784 ENTREZ_GENE Ralgapa1 2310003F20Rik|4930400K19Rik|AI563624|GRIPE|Garnl1|Tulip1|mKIAA0884 10090 Mus musculus 0.915 - - - - NO BioGRID ORCS
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84 237339 ENTREZ_GENE L3mbtl3 AI481284|MBT-1 10090 Mus musculus 0.938 - - - - NO BioGRID ORCS
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84 225055 ENTREZ_GENE Fbxo11 C80048|Jf 10090 Mus musculus 0.94 - - - - NO BioGRID ORCS
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84 69917 ENTREZ_GENE Nabp2 2610036N15Rik|Obfc2b|SSB1 10090 Mus musculus 0.941 - - - - NO BioGRID ORCS
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84 20937 ENTREZ_GENE Suv39h1 AI852103|AL022883|DXHXS7466e|H3-K9-HMTase 1|KMT1A|mIS6 10090 Mus musculus 0.947 - - - - NO BioGRID ORCS
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84 74585 ENTREZ_GENE Sppl3 4833416I09Rik|Psl4|Usmg3 10090 Mus musculus 0.959 - - - - NO BioGRID ORCS
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84 23924 ENTREZ_GENE Katna1 - 10090 Mus musculus 0.964 - - - - NO BioGRID ORCS
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84 74200 ENTREZ_GENE 2810403A07Rik A430106P18Rik|AI256352|AI451678|Blom7|Kiaa0907 10090 Mus musculus 0.979 - - - - NO BioGRID ORCS
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84 102595 ENTREZ_GENE Plekho2 AI840980|Plekhq1 10090 Mus musculus 0.983 - - - - NO BioGRID ORCS
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84 17168 ENTREZ_GENE Nprl3 Aag|CGTHBA|HS-26|HS-40|Mare|Phg|Prox1|m(alpha)RE 10090 Mus musculus 0.988 - - - - NO BioGRID ORCS
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84 30841 ENTREZ_GENE Kdm2b Cxxc2|Fbl10|Fbxl10|Jhdm1b|PCCX2 10090 Mus musculus 0.994 - - - - NO BioGRID ORCS
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84 63872 ENTREZ_GENE Zfp296 2210018A16Rik|Znf296 10090 Mus musculus 1.001 - - - - NO BioGRID ORCS
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84 56878 ENTREZ_GENE Rbms1 2600014B10Rik|AI255215|MSSP-1|MSSP-2|MSSP-3|YC1 10090 Mus musculus 1.007 - - - - NO BioGRID ORCS
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84 238317 ENTREZ_GENE Elmsan1 9430029N19Rik|C130039O16Rik|Gm260 10090 Mus musculus 1.015 - - - - NO BioGRID ORCS
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84 77044 ENTREZ_GENE Arid2 1700124K17Rik|4432409D24Rik|zipzap/p200 10090 Mus musculus 1.016 - - - - NO BioGRID ORCS
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84 22019 ENTREZ_GENE Tpp2 TPP-2|TppII 10090 Mus musculus 1.034 - - - - NO BioGRID ORCS
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84 231821 ENTREZ_GENE Adap1 4930431P11Rik|Centa1|GC1L|p42IP4 10090 Mus musculus 1.039 - - - - NO BioGRID ORCS
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84 16993 ENTREZ_GENE Lta4h - 10090 Mus musculus 1.055 - - - - NO BioGRID ORCS
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84 19664 ENTREZ_GENE Rbpj AI843960|CBF1|Igkjrb|Igkrsbp|RBP-J|RBP-J kappa|RBP-Jkappa|RBPjk|Rbpsuh 10090 Mus musculus 1.065 - - - - NO BioGRID ORCS
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84 224617 ENTREZ_GENE Tbc1d24 9630033P11|C530046L02Rik|mKIAA1171 10090 Mus musculus 1.078 - - - - NO BioGRID ORCS
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84 56032 ENTREZ_GENE Nprl2 2810446G01Rik|G21|NPR2L|Tusc4 10090 Mus musculus 1.097 - - - - NO BioGRID ORCS
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84 30046 ENTREZ_GENE Zfp292 5730450D02Rik|5830493J20Rik|9430062L07Rik|AI449016|Krox-10|Zfp-15|Zfp15|Zn-15|Zn-16|mKIAA0530 10090 Mus musculus 1.103 - - - - NO BioGRID ORCS
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84 108837 ENTREZ_GENE Ibtk 5430411K16Rik|AA409502|BTKI|mKIAA1417 10090 Mus musculus 1.108 - - - - NO BioGRID ORCS
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84 12914 ENTREZ_GENE Crebbp AW558298|CBP|CBP/p300|KAT3A|p300/CBP 10090 Mus musculus 1.113 - - - - NO BioGRID ORCS
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84 14784 ENTREZ_GENE Grb2 AA408164|Ash 10090 Mus musculus 1.118 - - - - NO BioGRID ORCS
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84 76901 ENTREZ_GENE Jade2 1200017K05Rik|AI480685|Phf15|mKIAA0239 10090 Mus musculus 1.118 - - - - NO BioGRID ORCS
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84 20527 ENTREZ_GENE Slc2a3 AA408729|AL023014|AL024341|AU040424|C78366|Glut-3|Glut3 10090 Mus musculus 1.133 - - - - NO BioGRID ORCS
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84 26992 ENTREZ_GENE Brd7 BP75|CELTIX1|Ptpn13ip 10090 Mus musculus 1.148 - - - - NO BioGRID ORCS
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84 76890 ENTREZ_GENE Memo1 0610016J10Rik|D930048L02Rik 10090 Mus musculus 1.188 - - - - NO BioGRID ORCS
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84 56637 ENTREZ_GENE Gsk3b 7330414F15Rik|8430431H08Rik|C86142|GSK-3|GSK-3beta|GSK3 10090 Mus musculus 1.191 - - - - NO BioGRID ORCS
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84 100233208 ENTREZ_GENE Gm10778 - 10090 Mus musculus 1.218 - - - - NO BioGRID ORCS
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84 76233 ENTREZ_GENE Dnttip1 6430706C13Rik 10090 Mus musculus 1.235 - - - - NO BioGRID ORCS
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84 16563 ENTREZ_GENE Kif2a C530030B14Rik|Kif2|Kns2|M-kinesin 10090 Mus musculus 1.25 - - - - NO BioGRID ORCS
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84 17859 ENTREZ_GENE Mxi1 Gm10197|Mad2|bHLHc11 10090 Mus musculus 1.253 - - - - NO BioGRID ORCS
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84 110794 ENTREZ_GENE Cebpe C/EBPe|CRP1|Gm294 10090 Mus musculus 1.262 - - - - NO BioGRID ORCS
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84 654362 ENTREZ_GENE Fbxw7as1 Dear|Dear1|Despr 10090 Mus musculus 1.262 - - - - NO BioGRID ORCS
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84 103537 ENTREZ_GENE Mbtd1 AA408199|AI194990|hemp 10090 Mus musculus 1.274 - - - - NO BioGRID ORCS
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84 67579 ENTREZ_GENE Cpeb4 Cpe-bp4|Cpeb-4|mCPEB-4 10090 Mus musculus 1.316 - - - - NO BioGRID ORCS
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84 50754 ENTREZ_GENE Fbxw7 1110001A17Rik|AGO|Cdc4|Fbw7|Fbwd6|Fbx30|Fbxo30|Fbxw6|SEL-10 10090 Mus musculus 1.371 - - - - NO BioGRID ORCS
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84 56484 ENTREZ_GENE Foxo3 1110048B16Rik|2010203A17Rik|C76856|FKHRL1|Fkhr2|Foxo3a 10090 Mus musculus 1.395 - - - - NO BioGRID ORCS
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84 66923 ENTREZ_GENE Pbrm1 2610016F04Rik|AI507524|BAF180|Pb1 10090 Mus musculus 1.421 - - - - NO BioGRID ORCS
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84 14359 ENTREZ_GENE Fxr1 1110050J02Rik|9530073J07Rik|AI851072|Fxr1h|Fxr1p 10090 Mus musculus 1.438 - - - - NO BioGRID ORCS
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84 64930 ENTREZ_GENE Tsc1 - 10090 Mus musculus 1.523 - - - - NO BioGRID ORCS
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84 74747 ENTREZ_GENE Ddit4 5830413E08Rik|AA415483|REDD1|Rtp801|dig2 10090 Mus musculus 1.584 - - - - NO BioGRID ORCS
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84 101739 ENTREZ_GENE Psip1 AA408851|AU015605|Dfs70|Ledgfa|Ledgfb|Psip2 10090 Mus musculus 1.628 - - - - NO BioGRID ORCS
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84 320376 ENTREZ_GENE Bcorl1 6720425J07Rik|B930011H20Rik 10090 Mus musculus 1.638 - - - - NO BioGRID ORCS
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84 69837 ENTREZ_GENE Pcgf1 2010002K04Rik|AU024121|Nspc1 10090 Mus musculus 1.646 - - - - NO BioGRID ORCS
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84 70294 ENTREZ_GENE Rnf126 2610010O19Rik 10090 Mus musculus 1.687 - - - - NO BioGRID ORCS
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84 17283 ENTREZ_GENE Men1 AW045611 10090 Mus musculus 1.704 - - - - NO BioGRID ORCS
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84 22059 ENTREZ_GENE Trp53 Tp53|bbl|bfy|bhy|p44|p53 10090 Mus musculus 1.792 - - - - NO BioGRID ORCS
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84 16911 ENTREZ_GENE Lmo4 A730077C12Rik|Crp3|Etohi4 10090 Mus musculus 1.852 - - - - NO BioGRID ORCS
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84 15902 ENTREZ_GENE Id2 AI255428|C78922|Idb2|bHLHb26 10090 Mus musculus 1.878 - - - - NO BioGRID ORCS
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84 66540 ENTREZ_GENE Fam107b 3110001A13Rik|AA589595|AA959793|AI132312 10090 Mus musculus 2.059 - - - - NO BioGRID ORCS
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84 12575 ENTREZ_GENE Cdkn1a CAP20|CDKI|CIP1|Cdkn1|P21|SDI1|Waf1|mda6|p21Cip1|p21WAF 10090 Mus musculus 2.29 - - - - NO BioGRID ORCS
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84 26413 ENTREZ_GENE Mapk1 9030612K14Rik|AA407128|AU018647|C78273|ERK|Erk2|MAPK2|PRKM2|Prkm1|p41mapk|p42mapk 10090 Mus musculus 2.395 - - - - NO BioGRID ORCS
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#SCREEN_ID SOURCE_ID SOURCE_TYPE AUTHOR SCREEN_NAME SCORES_SIZE FULL_SIZE FULL_SIZE_AVAILABLE NUMBER_OF_HITS ANALYSIS SIGNIFICANCE_INDICATOR SIGNIFICANCE_CRITERIA THROUGHPUT SCREEN_TYPE SCREEN_FORMAT EXPERIMENTAL_SETUP DURATION CONDITION_NAME CONDITION_DOSAGE MOI LIBRARY LIBRARY_TYPE LIBRARY_METHODOLOGY ENZYME CELL_LINE CELL_TYPE PHENOTYPE SCORE_COL_COUNT SCORE.1_TYPE SCORE.2_TYPE SCORE.3_TYPE SCORE.4_TYPE SCORE.5_TYPE ORGANISM_ID ORGANISM_OFFICIAL NOTES SOURCE SCREEN_RATIONALE
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81 28162770 pubmed Wang T (2017) 16-PMID28162770 18987 18987 Yes 3426 Log2 Fold Change (L2FC) Score Significance Score.1 (CRISPR Score (CS)) < -1.0 High Throughput Negative Selection Pool Timecourse 14 Doublings - - - Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library CRISPRn Knockout Cas9 Ba/F3 Pre-B-Lymphocyte Cell Line cell proliferation 1 CRISPR Score (CS) - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
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82 28162770 pubmed Wang T (2017) 17-PMID28162770 18987 18987 Yes 4759 Log2 Fold Change (L2FC) Score Significance Score.1 (CRISPR Score (CS)) < -1.0 High Throughput Negative Selection Pool Timecourse 14 Doublings - - - Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library CRISPRn Knockout Cas9 Ba/F3 Pre-B-Lymphocyte Cell Line cell proliferation 1 CRISPR Score (CS) - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
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83 28162770 pubmed Wang T (2017) 18-PMID28162770 18987 18987 Yes 3296 Log2 Fold Change (L2FC) Score Significance Score.1 (CRISPR Score (CS)) < -1.0 High Throughput Negative Selection Pool Timecourse 14 Doublings Murine Interleukin 3 1 ng/mL - Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library CRISPRn Knockout Cas9 Ba/F3 Pre-B-Lymphocyte Cell Line cell proliferation 1 CRISPR Score (CS) - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
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84 28162770 pubmed Wang T (2017) 19-PMID28162770 18987 18987 Yes 3761 Log2 Fold Change (L2FC) Score Significance Score.1 (CRISPR Score (CS)) < -1.0 High Throughput Negative Selection Pool Timecourse 14 Doublings Murine Interleukin 3 1 ng/mL - Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library CRISPRn Knockout Cas9 Ba/F3 Pre-B-Lymphocyte Cell Line cell proliferation 1 CRISPR Score (CS) - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
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161 29290584 pubmed Pusapati GV (2017) 1-PMID29290584 19674 19674 Yes 3053 MaGeCK Score Significance Score.1 (p-Value) < 0.1 High Throughput Phenotype Screen Pool Timecourse 24 Hours - - = 0.3 Brie (mouse) CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line regulation of signal transduction phenotype 3 p-Value FDR Log2 - - 10090 M. musculus Screen for negative regulators of the Hedgehog signaling pathway BioGRID ORCS Negative regulators of sonic hedgehog signalling pathway
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163 29290584 pubmed Pusapati GV (2017) 2-PMID29290584 19674 19674 Yes 3020 MaGeCK Score Significance Score.1 (p-Value) < 0.1 High Throughput Phenotype Screen Pool Ligand Exposure 24 Hours Sonic hedgehog protein (mouse) 3.2 nM = 0.3 Brie (mouse) CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line regulation of signal transduction phenotype 3 p-Value FDR Log2 - - 10090 M. musculus Screen for negative regulators of the Hedgehog signaling pathway BioGRID ORCS Negative regulators of sonic hedgehog signalling pathway
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165 29290584 pubmed Pusapati GV (2017) 3-PMID29290584 19674 19674 Yes 2383 MaGeCK Score Significance Score.1 (p-Value) < 0.1 High Throughput Phenotype Screen Pool Ligand Exposure 24 Hours Sonic hedgehog protein (mouse) 25 nM = 0.3 Brie (mouse) CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line regulation of signal transduction phenotype 3 p-Value FDR Log2 - - 10090 M. musculus Screen for negative regulators of the Hedgehog signaling pathway BioGRID ORCS Negative regulators of sonic hedgehog signalling pathway
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167 29290584 pubmed Pusapati GV (2017) 4-PMID29290584 19674 19674 Yes 2377 MaGeCK Score Significance Score.1 (p-Value) < 0.1 High Throughput Phenotype Screen Pool Ligand Exposure 24 Hours Sonic hedgehog protein (mouse) 25 nM = 0.3 Brie (mouse) CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line regulation of signal transduction phenotype 3 p-Value FDR Log2 - - 10090 M. musculus Screen for positive regulators of the Hedgehog signaling pathway BioGRID ORCS Positive regulators of sonic hedgehog signalling pathway
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345 29459677 pubmed Breslow DK (2018) 2-PMID29459677 22414 22414 Yes 1516 CasTLE Column Significance - High Throughput Negative Selection Pool Timecourse 15 Days - - ~ 0.3 Bassik Mouse CRISPR Knockout Library CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line cell proliferation 3 CasTLE Score CasTLE Effect p-Value - - 10090 M. musculus Genes whose depletion caused a growth defect were determined so that they could be excluded from Hedgehog pathway specific analyses. BioGRID ORCS Cell-essential genes
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578 27760321 pubmed Tzelepis K (2016) 8-PMID27760321 19149 19149 Yes 360 MaGeCK Score Significance Score.2 (FDR) < 0.1 High Throughput Negative Selection Pool Timecourse 14 Days - - - Murine lentiviral gRNA library (version 1) CRISPRn Knockout Cas9 JM8.F6 Embryonic Stem Cell Line cell proliferation 2 MaGeCK Score FDR - - - 10090 M. musculus Depleted genes were identified at a cutoff of FDR 10% (FDR < 0.1). BioGRID ORCS Cell-essential genes
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579 27760321 pubmed Tzelepis K (2016) 9-PMID27760321 18424 18424 Yes 1680 MaGeCK Score Significance Score.2 (FDR) < 0.1 High Throughput Negative Selection Pool Timecourse 14 Days - - - Mouse improved genome-wide library v2 CRISPRn Knockout Cas9 JM8.F6 Embryonic Stem Cell Line cell proliferation 2 MaGeCK Score FDR - - - 10090 M. musculus Depleted genes were identified at a cutoff of FDR 10% (FDR < 0.1). BioGRID ORCS Cell-essential genes
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599 28723893 pubmed Manguso RT (2017) 1-PMID28723893 455 2368 No 62 STARS Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen in vivo Implantation to Mouse Model 12 Days Immunotherapy (GVAX + PD-1 blockade) - - CRISPRn Targeted Library (Haining, 2017) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line tumorigenicity 4 STARS Score p-Value FDR q-Value - 10090 M. musculus sgRNAs that are significantly depleted from tumours treated with immunotherapy ( GVAX + PD-1 blockade) relative to TCRalpha KO mice which are unable to apply adaptive immune-selective pressure on the tumours, indicate that loss of the targeted gene increased the sensitivity of tumour cells to immune attack|The immunotherapy consisted of animals injected with tumour cells being treated with a granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting, irradiated tumour cell vaccine (GVAX) combined with PD-1 blockade using a monoclonal antibody against PD-1 to generate an adaptive immune response sufficient to apply immune-selective pressure on the tumour cells BioGRID ORCS Tumorigenicity in presence of immunotherapy
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615 28723893 pubmed Manguso RT (2017) 2-PMID28723893 436 2368 No 38 STARS Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen in vivo Implantation to Mouse Model 12 Days Immunotherapy (GVAX + PD-1 blockade) - - CRISPRn Targeted Library (Haining, 2017) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line tumorigenicity 4 STARS Score p-Value FDR q-Value - 10090 M. musculus sgRNAs that are significantly enriched from tumours treated with immunotherapy ( GVAX + PD-1 blockade) relative to TCRalpha KO mice which are unable to apply adaptive immune-selective pressure on the tumours, indicate that loss of the targeted gene decreased the sensitivity of tumour cells to immune attack|The immunotherapy consisted of animals injected with tumour cells being treated with a granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting, irradiated tumour cell vaccine (GVAX) combined with PD-1 blockade using a monoclonal antibody against PD-1 to generate an adaptive immune response sufficient to apply immune-selective pressure on the tumour cells BioGRID ORCS Tumorigenicity in presence of immunotherapy
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616 28723893 pubmed Manguso RT (2017) 3-PMID28723893 454 2368 No 55 STARS Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen in vivo Implantation to Mouse Model 12 Days Immunotherapy (GVAX only) - - CRISPRn Targeted Library (Haining, 2017) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line tumorigenicity 4 STARS Score p-Value FDR q-Value - 10090 M. musculus sgRNAs that are significantly depleted from tumours treated with the immunotherapy cancer vaccine GVAX relative to TCRalpha KO mice which lack CD4+ and CD8+ T cells and are unable to apply adaptive immune-selective pressure on the tumours, indicate that loss of the targeted gene increased the sensitivity of tumour cells to GVAX|GVAX is a vaccine composed of whole tumor cells genetically modified to secrete the immune stimulatory cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), and then irradiated to prevent further cell division to generate an adaptive immune response sufficient to apply immune-selective pressure on tumour cells BioGRID ORCS Tumorigenicity in presence of immunotherapy
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617 28723893 pubmed Manguso RT (2017) 4-PMID28723893 456 2368 No 17 STARS Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen in vivo Implantation to Mouse Model 12 Days Immunotherapy (GVAX only) - - CRISPRn Targeted Library (Haining, 2017) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line tumorigenicity 4 STARS Score p-Value FDR q-Value - 10090 M. musculus sgRNAs that are significantly enriched in tumours treated with the immunotherapy cancer vaccine GVAX relative to TCRalpha KO mice which lack CD4+ and CD8+ T cells and are unable to apply adaptive immune-selective pressure on the tumours, indicate that loss of the targeted gene decreased the sensitivity of tumour cells to GVAX|GVAX is a vaccine composed of whole tumor cells genetically modified to secrete the immune stimulatory cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), and then irradiated to prevent further cell division to generate an adaptive immune response sufficient to apply immune-selective pressure on tumour cells BioGRID ORCS Tumorigenicity in presence of immunotherapy
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684 29478914 pubmed Yamauchi T (2018) 1-PMID29478914 1714 20611 No 1714 MaGeCK All Significant - High Throughput Negative Selection Pool Timecourse 16 Days - - - GECKO v2 (mouse) CRISPRn Knockout Cas9 MLL/AF9 Cas9 Acute Myeloid Leukemia Cell Line cell proliferation 1 FDR - - - - 10090 M. musculus authors reported genes that met a cut-off of FDR<0.25 as determined my Mageck BioGRID ORCS Cell-essential genes
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685 29478914 pubmed Yamauchi T (2018) 2-PMID29478914 1722 20611 No 1722 MaGeCK All Significant - High Throughput Negative Selection Pool Timecourse 16 Days - - - GECKO v2 (mouse) CRISPRn Knockout Cas9 CALM/AF10 Cas9 Acute Myeloid Leukemia Cell Line cell proliferation 1 FDR - - - - 10090 M. musculus authors reported genes that met a cut-off of FDR<0.25 as determined my Mageck BioGRID ORCS Cell-essential genes
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687 30797773 pubmed Codina A (2019) 1-PMID30797773 19590 19590 Yes 9 MaGeCK Column Significance - High Throughput Positive Selection in vivo Implantation to Mouse Model 90 Days - - ~ 0.3 Brie (mouse) CRISPRn Knockout Cas9 IMC9 Immortal Mouse Liver-derived Cell Line tumorigenicity 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus a p53-/-;Myc-primed mouse hepatocyte cell line was transfected with the Brie CRISPR library and implanted into a Nu/Nu mouse background. Genes whose knockout induced tumor formation based on the results of 3 separate analyses methods (RIGER, 3-way HitCaller and MAGECK) were considered high confidence screen hits BioGRID ORCS Tumorigenicity
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688 30797773 pubmed Codina A (2019) 2-PMID30797773 21288 21288 Yes 4 MaGeCK Column Significance - High Throughput Positive Selection in vivo Implantation to Mouse Model 90 Days - - ~ 0.3 GECKO v2 (mouse) CRISPRn Knockout Cas9 IMC9 Immortal Mouse Liver-derived Cell Line tumorigenicity 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus a p53-/-;Myc-primed mouse hepatocyte cell line was transfected with the mGECKO CRISPR library and implanted into a Nu/Nu mouse background. Genes whose knockout induced tumor formation based on the results of 3 separate analyses methods (RIGER, 3-way HitCaller and MAGECK) were considered high confidence screen hits BioGRID ORCS Tumorigenicity
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690 30905739 pubmed Yang J (2019) 1-PMID30905739 142 19994 No 142 None All Significant - High Throughput Phenotype Screen Pool Timecourse 16 Days - - = 0.3 CRISPRa (Metzakopian, 2019) CRISPRa Activation SAM (NLS-dCas9-VP64/MS2-p65-HSF1) EpiSC (primed epiblast) Mouse cell protein/peptide accumulation 1 Read counts - - - - 10090 M. musculus authors used an Oct4-GFP reporter and maintained Oct4 expression to determine reprogramming state BioGRID ORCS Activation of the gene induces reprogramming in a differentiated cell
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1043 31253397 pubmed Yazawa R (2019) 1-PMID31253397 698 18424 No 698 None All Significant - High Throughput Phenotype Screen Pool Timecourse 24 Hours Starvation - - Mouse improved genome-wide library v2 CRISPRn Knockout Cas9 MEF Embryonic Fibroblast Cell Line autophagy 1 Read counts - - - - 10090 M. musculus Cas9 MEF cells expressing a tagged autophagy reporter (pHlurin-LC3-mCherry) were transduced with the genome-wide gRNA library and starved. Cells with a high pHluorin/mCherry ratio, indicating the impairment of autophagic flux, were enriched and next-generation sequencing (NGS) was used to identify integrated gRNA region which identified 1303 genes involved in autophagic activity in two replicate experiments BioGRID ORCS Regulators of autophagy
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1044 31253397 pubmed Yazawa R (2019) 2-PMID31253397 909 18424 No 909 None All Significant - High Throughput Phenotype Screen Pool Timecourse 24 Hours Starvation - - Mouse improved genome-wide library v2 CRISPRn Knockout Cas9 MEF Embryonic Fibroblast Cell Line autophagy 1 Read counts - - - - 10090 M. musculus Cas9 MEF cells expressing a tagged autophagy reporter (pHlurin-LC3-mCherry) were transduced with the genome-wide gRNA library and starved. Cells with a high pHluorin/mCherry ratio, indicating the impairment of autophagic flux, were enriched and next-generation sequencing (NGS) was used to identify integrated gRNA region which identified 1303 genes involved in autophagic activity in two replicate experiments BioGRID ORCS Regulators of autophagy
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1047 30932271 pubmed Yu JSL (2019) 1-PMID30932271 18424 18424 Yes 14 MaGeCK Score Significance Score.1 (Depletion-Enrichment (DE) score) > 3.0 High Throughput Phenotype Screen Pool Timecourse 17 Days Reprogramming conditions (5% O2, 2 μM SB431542/Alk5i) - = 0.3 Mouse improved genome-wide library v2 CRISPRn Knockout Cas9 primary cultured fibroblast Mouse cell protein/peptide accumulation 3 Depletion-Enrichment (DE) score p-Value p-Value - - 10090 M. musculus Authors screened Cas9 expressing primary cultured mouse cardiac fibroblasts for genes effecting the reprogramming of differentiated fibroblasts into multi-potent progenitor cells using the progenitor cell marker Nkx2-5 as a reporter|Authors used a MaGeCK derived p-values to generate a depletion-enrichment (DE) score for each gene. A positive DE score indicates that loss of that particular gene is advantageous for reprogramming. BioGRID ORCS Regulators of reprogramming and differentiation
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1077 30595499 pubmed Villegas F (2019) 1-PMID30595499 19138 19138 Yes 18 MaGeCK Score Significance Score.3 (FDR) < 0.25 High Throughput Phenotype Screen Pool Timecourse 3 Days Differentiation conditions (absence of 2i) - = 0.25 Murine lentiviral gRNA library (version 1) CRISPRn Knockout Cas9 RGd2 ES Mouse Embryonic Stem Cell protein/peptide accumulation 3 MaGeCK Score p-Value FDR - - 10090 M. musculus authors used the presence of GFP-tagged Rex1 (a self-renewing ESC reporter) to screen for genes whose loss prevented differentiation BioGRID ORCS Regulators of differentiation
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1087 31744829 pubmed Li F (2019) 1-PMID31744829 727 727 Yes 75 MaGeCK Score Significance Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 High Throughput Phenotype Screen in vivo Implantation to Mouse Model 24 Days - - = 0.2 Targeted epigenetic regulator library (Wong, 2019) CRISPRn Knockout Cas9 KP-Cas9-Clone 7 Lung Cancer Cell Line tumorigenicity 5 MaGeCK Score p-Value MaGeCK Score p-Value Log2FC 10090 M. musculus - BioGRID ORCS Cancer cell response to immune response
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1089 31744829 pubmed Li F (2019) 2-PMID31744829 727 727 Yes 74 MaGeCK Score Significance Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 High Throughput Phenotype Screen in vivo Implantation to Mouse Model 24 Days Anti–PD-1 - = 0.2 Targeted epigenetic regulator library (Wong, 2019) CRISPRn Knockout Cas9 KP-Cas9-Clone 7 Lung Cancer Cell Line tumorigenicity 5 MaGeCK Score p-Value MaGeCK Score p-Value Log2FC 10090 M. musculus - BioGRID ORCS cancer cell response to anti-PD-1 immunotherapy
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1130 29459677 pubmed Breslow DK (2018) 1-PMID29459677 20898 20898 Yes 472 CasTLE Score Significance Score.4 (FDR) = 1.0 High Throughput Phenotype Screen Pool Ligand Exposure 5 Days Sonic hedgehog protein (mouse) - ~ 0.3 Bassik Mouse CRISPR Knockout Library CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line regulation of signal transduction phenotype 4 CRISPR Score (CS) CasTLE Effect p-Value FDR - 10090 M. musculus The authors screened for genes affecting ciliary Hh signaling using a a reporter that converts Hh signaling into antibiotic resistance. Genes with a positive CasTLE effect increase Hh pathway signaling while genes with a negative CasTLE effect decrease Hh pathway signaling BioGRID ORCS Regulators of Hh signaling pathway
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1133 29769725 pubmed Zhang R (2018) 1-PMID29769725 21545 21545 Yes 1118 MaGeCK Score Significance Score.2 (p-Value) < 0.05 High Throughput Positive Selection Pool Virus Exposure 24 Hours Virus: CHIKV (Chikungunya virus) 1 MOI = 0.3 GECKO v2 (mouse) CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line response to virus 3 MaGeCK Score p-Value FDR - - 10090 M. musculus Phenotypic readout: cell proliferation|CHIKV is short for Chikungunya virus strain Senegal 37997 BioGRID ORCS Increased resistance to virus
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1152 31209294 pubmed Fu X (2019) 1-PMID31209294 20664 20664 Yes 486 MaGeCK Score Significance Score.2 (neg score p-value) < 0.01 OR Score.4 (pos score p-value) < 0.01 High Throughput Phenotype Screen Pool Other 1 Days Dux gene induction - - Brie (mouse) CRISPRn Knockout Cas9 Mouse Embryonic Stem Cell Embryonic Cell Line protein/peptide accumulation 5 MAGeCK neg score neg score p-value MAGeCK pos score pos score p-value Log2FC 10090 M. musculus Genes whose KO results in a negative MAGeCK score associated with a significant p-value are depleted in the totipotent population, suggesting they are positive regulators of totipotency.|Genes whose KO results in a positive MAGeCK score associated with a significant p-value are enriched in the totipotent population, suggesting they are negative regulators of totipotency. BioGRID ORCS Regulators of totipotency
|
1157 29301958 pubmed Pan D (2018) 1-PMID29301958 325 19674 No 325 MaGeCK Score Significance Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 3 Days Pmel-1 T cells - = 0.06 Brie (mouse) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line cell proliferation 5 Log2 p-Value FDR p-Value FDR 10090 M. musculus Phenotypic readout: cell proliferation|The authors screened for gene knockouts in a melanoma cell line that affect sensitivity to killing by cytotoxic Pmel-1 T cells, which have a relatively low TCR affinity for an endogenous melanoma antigen|Knock out of genes with a positive log fold change score results in increased resistance to Pmel-1 T cells. A negative log fold change score indicates that knock out of the gene resulted in increased sensitivity to Pmel-1 cells BioGRID ORCS Tumor response to cytotoxic T-cells
|
1158 29301958 pubmed Pan D (2018) 2-PMID29301958 174 19674 No 174 MaGeCK Score Significance Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 1 Days OT-I T cells - = 0.06 Brie (mouse) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line cell proliferation 5 Log2 p-Value FDR p-Value FDR 10090 M. musculus Phenotypic readout: cell proliferation|The authors screened for gene knockouts in a melanoma cell line that conferred increased resistance to killing by cytotoxic OT-I T cells, which have a high TCR affinity for an endogenous melanoma antigen|Knock out of genes with a positive log fold change score results in increased resistance to OT-I T cells. A negative log fold change score indicates that knock out of the gene resulted in increased sensitivity to OT-I T cells BioGRID ORCS Tumor response to cytotoxic T-cells
|
1197 28768188 pubmed Okada M (2017) 1-PMID28768188 100 19150 No 42 FDR Score Significance Score.1 (FDR) < 0.15 High Throughput Phenotype Screen Pool Timecourse 10 Days - - = 0.5 Murine lentiviral gRNA library (version 1) CRISPRn Knockout Cas9 68-41-Cas9 T cell protein/peptide accumulation 1 FDR - - - - 10090 M. musculus T cell exhaustion is associated with high expression of PD-1|KO of hit genes leads to low PD-1 expression, suggesting the gene normally activates PD-1 expression, contributing to T cell exhaustion BioGRID ORCS Regulators of PD-1 expression
|
1259 32968282 pubmed Lawson KA (2020) 1-PMID32968282 19466 19466 Yes 1865 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 15 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1260 32968282 pubmed Lawson KA (2020) 2-PMID32968282 19466 19466 Yes 1982 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 24 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1261 32968282 pubmed Lawson KA (2020) 3-PMID32968282 19466 19466 Yes 1912 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 14 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1262 32968282 pubmed Lawson KA (2020) 4-PMID32968282 19466 19466 Yes 1888 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 25 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1263 32968282 pubmed Lawson KA (2020) 5-PMID32968282 19466 19466 Yes 1989 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 11 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1264 32968282 pubmed Lawson KA (2020) 6-PMID32968282 19466 19466 Yes 2141 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 17 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1265 32968282 pubmed Lawson KA (2020) 7-PMID32968282 19466 19466 Yes 1743 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 11 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 EMT6 Mammary Gland Tumor Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1266 32968282 pubmed Lawson KA (2020) 8-PMID32968282 19466 19466 Yes 1907 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 19 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 EMT6 Mammary Gland Tumor Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1267 32968282 pubmed Lawson KA (2020) 9-PMID32968282 19466 19466 Yes 1736 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 12 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 MC38-OVA Colonic Adenocarcinoma Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1268 32968282 pubmed Lawson KA (2020) 10-PMID32968282 19466 19466 Yes 1713 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 15 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 MC38-OVA Colonic Adenocarcinoma Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
1269 32968282 pubmed Lawson KA (2020) 11-PMID32968282 19466 19466 Yes 1919 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 13 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 B16-MO4 Melanoma Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
|
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