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1784 33846634 pubmed Inoue D (2021) 1-PMID33846634 34 593 No 34 EdgeR All Significant - High Throughput Phenotype Screen Pool Cytokine depletion 7 Days IL-3 withdrawal - - CRISPRn ZRSR2 splicing target library (Abdel-Wahab, 2021) CRISPRn Knockout Cas9 Ba/F3 Pre-B-Lymphocyte Cell Line regulation of signal transduction phenotype 3 Log2FC p-Value FDR - - 10090 M. musculus Genes with a positive LFC are genes whose loss conferred cytokine-independent growth. Genes with a negative LFC are genes whose loss conferred decreased growth in the absence of cytokine signal BioGRID ORCS Regulation of cytokine-independent growth
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1785 33846634 pubmed Inoue D (2021) 2-PMID33846634 50 593 No 50 EdgeR All Significant - High Throughput Phenotype Screen Pool Cytokine depletion 7 Days IL-3 withdrawal - - CRISPRn ZRSR2 splicing target library (Abdel-Wahab, 2021) CRISPRn Knockout Cas9 32Dcl3 bone marrow cell line regulation of signal transduction phenotype 3 Log2FC p-Value FDR - - 10090 M. musculus Genes with a positive LFC are genes whose loss conferred cytokine-independent growth. Genes with a negative LFC are genes whose loss conferred decreased growth in the absence of cytokine signal BioGRID ORCS Regulation of cytokine-independent growth
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1838 31509742 pubmed Freeman AJ (2019) 1-PMID31509742 20229 20229 Yes 1048 MaGeCK Score Significance Score.2 (p-Value) < 0.05 High Throughput Negative Selection Pool NK cell exposure 3 Days NK (activated natural killer) cells 1 Ratio of effector cells/target cells = 0.3 Brie (mouse) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line cell proliferation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Authors screened for tumor-specific genes that are associated with NK cell sensitivity|Hit genes increased sensitivity to NK cell exposure upon KO BioGRID ORCS Tumor cell response to NK cells
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1839 31509742 pubmed Freeman AJ (2019) 2-PMID31509742 20570 20570 Yes 1066 MaGeCK Score Significance Score.2 (p-Value) < 0.05 High Throughput Phenotype Screen Pool Cytokine exposure 1 Days Interferon gamma 1.0 ng/mL = 0.3 Brie (mouse) CRISPRn Knockout Cas9 B16-F10 Melanoma Cell Line protein/peptide accumulation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes show decreased MHC I expression after IFNgamma exposure upon KO BioGRID ORCS Regulation of MHC I expression after IFNgamma exposure
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1888 34497417 pubmed Kamber RA (2021) 3-PMID34497417 22898 22898 Yes 372 CasTLE Column Significance - High Throughput Phenotype Screen Pool Other 24 Hours Exposure to magnetic IgG-coated bead - - Bassik Mouse CRISPR Knockout Library CRISPRn Knockout Cas9 J774 macrophage phagocytosis 3 CasTLE Score CasTLE Effect p-Value - - 10090 M. musculus Hit genes with a negative castle effect are those whose KO blocks phagocytosis, hit genes with a positive castle effect are those whose KO increases phagocytosis BioGRID ORCS Regulators of phagocytosis by macrophages
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1889 34497417 pubmed Kamber RA (2021) 4-PMID34497417 22893 22893 Yes 38 CasTLE Column Significance - High Throughput Phenotype Screen Pool Other 72 Hours Exposure to APMAP-KO cancer cells - - Bassik Mouse CRISPR Knockout Library CRISPRn Knockout Cas9 J774 macrophage phagocytosis 3 CERES score CasTLE Effect p-Value - - 10090 M. musculus KO of hit genes with a negative castle effect results in decreased phagocytosis of APMAP-KO cancer cells|KO of hit genes with a positive castle effect results in increased phagocytosis of APMAP-KO cancer cells BioGRID ORCS Regulators of antibody-dependent cellular phagocytosis of APMAP-KO cancer cells
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1890 34497417 pubmed Kamber RA (2021) 5-PMID34497417 22894 22894 Yes 24 CasTLE Column Significance - High Throughput Phenotype Screen Pool Other 72 Hours Exposure to APMAP-KO cancer cells vs. Control-KO cancer cells - - Bassik Mouse CRISPR Knockout Library CRISPRn Knockout Cas9 J774 macrophage phagocytosis 3 CasTLE Score CasTLE Effect p-Value - - 10090 M. musculus KO of hit genes with a negative castle effect results in decreased phagocytosis of APMAP-KO, but not control-KO cancer cells|KO of hit genes with a positive castle effect results in increased phagocytosis of APMAP-KO, but not control-KO cancer cells BioGRID ORCS Regulators of selective antibody-dependent cellular phagocytosis of APMAP-KO cells
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1908 35263388 pubmed Ortega-Gonzalez P (2022) 1-PMID35263388 873 20077 No 873 STARS All Significant - High Throughput Positive Selection Pool Virus Exposure 9 Days Virus: Reovirus strains T1L or T3D 100.0 PFU/cell = 0.2 Asagio (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line response to virus 4 STARS Score p-Value FDR q-Value - 10090 M. musculus KO of the hit gene results in greater resistance to virus BioGRID ORCS Increased resistance to virus
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1937 35842423 pubmed Zou Y (2022) 1-PMID35842423 79 20611 No 79 Log2 Fold Change (L2FC) All Significant - High Throughput Positive Selection Pool Cytokine exposure | Drug exposure 48 Hours TNF‐α | Z-VAD-FMK 20 µM < 0.3 GECKO v2 (mouse) CRISPRn Knockout Cas9 NCTC clone 929 Fibroblast Cell Line viability 2 Log2FC p-Value - - - 10090 M. musculus KO of the hit gene results in increased resistance to necroptisis inducing factors BioGRID ORCS Regulation of TNF-induced necroptisis
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2019 33033111 pubmed Rushworth LK (2020) 1-PMID33033111 17 20611 No 17 MaGeCK All Significant - High Throughput Negative Selection in vivo Drug Exposure - docetaxel 6.0 mg/kg < 0.4 GECKO v2 (mouse) CRISPRn Knockout Cas9 SP1 Breast Cancer Cell Line response to chemicals 2 p-Value Log2FC - - - 10090 M. musculus Hit genes are those that are essential to survive docetaxel treatment BioGRID ORCS Increased resistance to drug
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2049 33513212 pubmed McLellan LK (2021) 1-PMID33513212 20144 20144 Yes 9 Log2 Fold Change (L2FC) Score Significance Score.1 (p-Value) > 2.58 AND Score.2 (Log2FC) > 0.0 High Throughput Phenotype Screen Pool Bacteria Exposure 75 Minutes Escherichia coli (str. UTI89) 150.0 MOI = 0.5 Brie (mouse) CRISPRn Knockout Cas9 IMCD-3 renal medulla cell line response to bacteria 2 p-Value Log2FC - - - 10090 M. musculus KO of hit genes results in decreased binding of uropathogenic Escherichia coli to kidney cells in vitro BioGRID ORCS Decreased binding of bacteria
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2127 35750052 pubmed Belk JA (2022) 1-PMID35750052 17447 17447 Yes 99 Z-score Score Significance Score.3 (FDR) < 0.06 High Throughput Positive Selection Pool Other 10 Days Chronic T cell stimulation - - Teichmann Retroviral Mouse Genome-wide CRISPR Knockout Library CRISPRn Knockout Cas9 CD8+ T s (primary-derived) Regulatory T cell cell proliferation 3 Z-score p-Value FDR - - 10090 M. musculus Hit genes with a positive z-score are enriched in chronically stimulated T cells as opposed to acutely stimulated T-cells BioGRID ORCS Regulation of T cell exhaustion
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2141 35379808 pubmed Frey N (2022) 1-PMID35379808 66 19647 No 66 MaGeCK All Significant - High Throughput Positive and Negative Selection Pool T cell exposure 3 Days OT-I T cells 1.0 Ratio of effector cells/target cells = 0.3 Brie (mouse) CRISPRn Knockout Cas9 KPC Pancreatic Ductal Adenocarcinoma Cell Line cell proliferation 5 Log2FC p-Value FDR p-Value FDR 10090 M. musculus Hit genes with a positive logfc increase resistance of pancreatic cancer cells to cytotoxic T lymphocyte killing upon KO|Hit genes with a negative logfc sensitize pancreatic cancer cells to cytotoxic T lymphocyte killing upon KO BioGRID ORCS Tumor cell survival in the presence of CD8+ T cells
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2142 35379808 pubmed Frey N (2022) 2-PMID35379808 64 63 No 64 MaGeCK All Significant - High Throughput Positive and Negative Selection Pool T cell exposure 5 Days OT-I T cells implanted into the pancreata of RAG1-/- mice - = 0.3 Targeted CRISPRn library (Schwank, 2022) CRISPRn Knockout Cas9 KPC Pancreatic Ductal Adenocarcinoma Cell Line cell proliferation 5 Log2FC p-Value FDR p-Value FDR 10090 M. musculus Hit genes with a positive logfc increase resistance of pancreatic cancer cells to cytotoxic T lymphocyte killing upon KO in the pancreata of RAG1-/- mice|Hit genes with a negative logfc sensitize pancreatic cancer cells to cytotoxic T lymphocyte killing upon KO in the pancreata of RAG1-/- mice BioGRID ORCS Tumor cell survival in the presence of CD8+ T cells in vivo
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2143 35972292 pubmed Sullender ME (2022) 1-PMID35972292 27 22774 No 27 Log2 Fold Change (L2FC) All Significant - High Throughput Positive Selection Pool Virus Exposure 10 Days MNV (murine norovirus) CR6 5.0 MOI ~ 0.4 Caprano CRISPRa Activation dCas9-VP64 BV-2 Microglial Cell Line response to virus 2 Log2FC p-Value - - - 10090 M. musculus Overexpression of the hit gene results in increased resistance to virus BioGRID ORCS Increased resistance to virus
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2144 35972292 pubmed Sullender ME (2022) 2-PMID35972292 28 22774 No 28 Log2 Fold Change (L2FC) All Significant - High Throughput Positive Selection Pool Virus Exposure 10 Days MNV (murine norovirus) 1 (CW3) 5.0 MOI ~ 0.4 Caprano CRISPRa Activation dCas9-VP64 BV-2 Microglial Cell Line response to virus 2 Log2FC p-Value - - - 10090 M. musculus Overexpression of the hit gene results in increased resistance to virus BioGRID ORCS Increased resistance to virus
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2170 34529489 pubmed Martin TD (2021) 1-PMID34529489 7253 7253 Yes 480 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Negative Selection Pool Timecourse 10 Doublings - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line cell proliferation 5 Rank MaGeCK Score p-Value FDR Log2FC 10090 M. musculus Hit genes are depleted upon KO in 4T1 cells grown in vitro BioGRID ORCS Cell-essential genes in vitro
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2171 34529489 pubmed Martin TD (2021) 2-PMID34529489 7253 7253 Yes 198 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Negative Selection in vivo Implantation to Mouse Model - - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 Rank MaGeCK Score p-Value FDR Log2FC 10090 M. musculus Hit genes are significantly depleted upon KO in a tumor sample grown in immunocompetent wildtype mice BioGRID ORCS Tumor cell survival
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2172 34529489 pubmed Martin TD (2021) 3-PMID34529489 7253 7253 Yes 282 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Negative Selection in vivo Implantation to Mouse Model - SCID BALB/c host - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 Rank MaGeCK Score p-Value FDR Log2FC 10090 M. musculus Hit genes are significantly depleted upon KO in a tumor sample grown in immunocompromised hosts BioGRID ORCS Tumor cell survival in an immunodeficient host
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2173 34529489 pubmed Martin TD (2021) 4-PMID34529489 7253 7253 Yes 40 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Positive Selection Pool Timecourse 10 Doublings - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line cell proliferation 5 Rank MaGeCK Score p-Value FDR Log2FC 10090 M. musculus Hit genes are enriched upon KO in 4T1 cells grown in vitro BioGRID ORCS Growth inhibition genes in vitro
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2174 34529489 pubmed Martin TD (2021) 5-PMID34529489 7253 7253 Yes 100 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Positive Selection in vivo Implantation to Mouse Model - - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 Rank MaGeCK Score p-Value FDR Log2FC 10090 M. musculus Hit genes are significantly enriched upon KO in a tumor sample grown in immunocompetent wildtype mice BioGRID ORCS Growth inhibition of tumor cells
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2175 34529489 pubmed Martin TD (2021) 6-PMID34529489 7253 7253 Yes 18 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Positive Selection in vivo Implantation to Mouse Model - SCID BALB/c host - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 Rank MaGeCK Score p-Value FDR Log2FC 10090 M. musculus Hit genes are significantly enriched upon KO in a tumor sample grown in a SCID BALB/c (immunocompromised) mice BioGRID ORCS Growth inhibition of tumor cells in an immunodeficient host
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2176 34529489 pubmed Martin TD (2021) 7-PMID34529489 7253 7253 Yes 44 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Negative Selection Pool Timecourse 10 Doublings - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line cell proliferation 4 Rank Log2FC p-Value FDR - 10090 M. musculus Hit genes are depleted upon KO in CT26 cells grown in vitro BioGRID ORCS Cell-essential genes in vitro
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2177 34529489 pubmed Martin TD (2021) 8-PMID34529489 7253 7253 Yes 44 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Negative Selection in vivo Implantation to Mouse Model - - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line tumorigenicity 4 Rank Log2FC p-Value FDR - 10090 M. musculus Hit genes are significantly depleted upon KO in a tumor sample grown in immunocompetent hosts BioGRID ORCS Tumor cell survival
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2178 34529489 pubmed Martin TD (2021) 9-PMID34529489 7253 7253 Yes 186 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Negative Selection in vivo Implantation to Mouse Model - SCID BALB/c host - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line tumorigenicity 4 Rank Log2FC p-Value FDR - 10090 M. musculus Hit genes are significantly depleted upon KO in a tumor sample grown in immunocompromised hosts BioGRID ORCS Tumor cell survival in an immunodeficient host
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2179 34529489 pubmed Martin TD (2021) 10-PMID34529489 7253 7253 Yes 54 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Positive Selection Pool Timecourse 10 Doublings - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line cell proliferation 4 Rank Log2FC p-Value FDR - 10090 M. musculus Hit genes are enriched upon KO in CT26 cells grown in vitro BioGRID ORCS Growth inhibition genes in vitro
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2180 34529489 pubmed Martin TD (2021) 11-PMID34529489 7253 7253 Yes 30 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Positive Selection in vivo Implantation to Mouse Model - - - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line tumorigenicity 4 Rank Log2FC p-Value FDR - 10090 M. musculus Hit genes are significantly enriched upon KO in a tumor sample grown in immunocompetent wildtype mice BioGRID ORCS Growth inhibition of tumor cells
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2181 34529489 pubmed Martin TD (2021) 12-PMID34529489 7253 7253 Yes 41 MaGeCK Score Significance Score.4 (FDR) < 0.1 High Throughput Positive Selection in vivo Implantation to Mouse Model - SCID BALB/c host - - Mouse druggable genome (Elledge, 2021) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line tumorigenicity 4 Rank Log2FC p-Value FDR - 10090 M. musculus Hit genes are significantly enriched upon KO in a tumor sample grown in a SCID BALB/c (immunocompromised) mice BioGRID ORCS Growth inhibition of tumor cells in an immunodeficient host
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2182 34529489 pubmed Martin TD (2021) 13-PMID34529489 472 472 Yes 47 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection Pool Timecourse 10 Doublings - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 185-3 MPNST Malignant Peripheral Nerve Sheath Tumor (MPNST) cancer cell cell proliferation 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in an in vitro population upon KO.|Hit genes with a significant negative log2fc are depleted in an in vitro population upon KO. BioGRID ORCS Cell-essential and growth restrictive genes
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2183 34529489 pubmed Martin TD (2021) 14-PMID34529489 472 472 Yes 18 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model - Rag1-/- mice - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 185-3 MPNST Malignant Peripheral Nerve Sheath Tumor (MPNST) cancer cell tumorigenicity 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in a tumor population grown in Rag1-/- hosts upon KO.|Hit genes with a significant negative log2fc are depleted in a tumor population grown in Rag1-/- hosts upon KO. BioGRID ORCS Tumor cell survival in immunodeficient host
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2184 34529489 pubmed Martin TD (2021) 15-PMID34529489 472 472 Yes 21 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model - - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 185-3 MPNST Malignant Peripheral Nerve Sheath Tumor (MPNST) cancer cell tumorigenicity 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in a tumor population grown in WT (immunocompetent) hosts upon KO.|Hit genes with a significant negative log2fc are depleted in a tumor population grown in WT (immunocompetent) hosts upon KO. BioGRID ORCS Tumor cell survival
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2185 34529489 pubmed Martin TD (2021) 16-PMID34529489 471 471 Yes 58 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection Pool Timecourse 10 Doublings - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 B16 Melanoma Cell Line cell proliferation 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in an in vitro population upon KO.|Hit genes with a significant negative log2fc are depleted in an in vitro population upon KO. BioGRID ORCS Cell-essential and growth restrictive genes
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2186 34529489 pubmed Martin TD (2021) 17-PMID34529489 471 471 Yes 36 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model - Rag1-/- mice - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 B16 Melanoma Cell Line tumorigenicity 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in a tumor population grown in Rag1-/- hosts upon KO.|Hit genes with a significant negative log2fc are depleted in a tumor population grown in Rag1-/- hosts upon KO. BioGRID ORCS Tumor cell survival in an immunodeficient host
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2187 34529489 pubmed Martin TD (2021) 18-PMID34529489 471 471 Yes 23 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model - - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 B16 Melanoma Cell Line tumorigenicity 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in a tumor population grown in WT (immunocompetent) hosts upon KO.|Hit genes with a significant negative log2fc are depleted in a tumor population grown in WT (immunocompetent) hosts upon KO. BioGRID ORCS Tumor cell survival
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2188 34529489 pubmed Martin TD (2021) 19-PMID34529489 472 472 Yes 31 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection Pool Timecourse 10 Doublings - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 KP1233 Non-Small Cell Lung Cancer Cell Line cell proliferation 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in an in vitro population upon KO.|Hit genes with a significant negative log2fc are depleted in an in vitro population upon KO. BioGRID ORCS Cell-essential and growth restrictive genes
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2189 34529489 pubmed Martin TD (2021) 20-PMID34529489 472 472 Yes 4 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model - Rag1-/- mice - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 KP1233 Non-Small Cell Lung Cancer Cell Line tumorigenicity 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in a tumor population grown in Rag1-/- hosts upon KO.|Hit genes with a significant negative log2fc are depleted in a tumor population grown in Rag1-/- hosts upon KO. BioGRID ORCS Tumor cell survival in an immunodeficient host
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2190 34529489 pubmed Martin TD (2021) 21-PMID34529489 472 472 Yes 0 EdgeR Score Significance Score.3 (FDR) < 0.1 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model - - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 KP1233 Non-Small Cell Lung Cancer Cell Line tumorigenicity 3 Log2FC p-Value FDR - - 10090 M. musculus Hit genes with a significant positive log2fc are enriched in a tumor population grown in WT (immunocompetent) hosts upon KO.|Hit genes with a significant negative log2fc are depleted in a tumor population grown in WT (immunocompetent) hosts upon KO. BioGRID ORCS Tumor cell survival
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2191 34529489 pubmed Martin TD (2021) 22-PMID34529489 472 472 Yes 13 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Negative Selection Pool T cell exposure 6 Hours OT-I T cells 0.1 Ratio of effector cells/target cells - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line cell proliferation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are depleted upon KO in C57BL/6 mouse pancreas cells expressing full-length ovalbumin exposed to cytotoxic OT-I CD8 cells at a ratio of 0.1 to 1 BioGRID ORCS Tumor cell survival in the presence of CD8+ T cells
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2192 34529489 pubmed Martin TD (2021) 23-PMID34529489 472 472 Yes 18 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Positive Selection Pool T cell exposure 6 Hours OT-I T cells 0.1 Ratio of effector cells/target cells - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line cell proliferation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are enriched upon KO in C57BL/6 mouse pancreas cells expressing full-length ovalbumin exposed to cytotoxic OT-I CD8 cells at a ratio of 0.1 to 1 BioGRID ORCS Tumor cell survival in the presence of CD8+ T cells
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2193 34529489 pubmed Martin TD (2021) 24-PMID34529489 472 472 Yes 2 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Negative Selection Pool T cell exposure 6 Hours OT-I T cells 0.25 Ratio of effector cells/target cells - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line cell proliferation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are depleted upon KO in C57BL/6 mouse pancreas cells expressing full-length ovalbumin exposed to cytotoxic OT-I CD8 cells at a ratio of 0.25 to 1 BioGRID ORCS Tumor cell survival in the presence of CD8+ T cells
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2194 34529489 pubmed Martin TD (2021) 25-PMID34529489 472 472 Yes 7 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Positive Selection Pool T cell exposure 6 Hours OT-I T cells 0.25 Ratio of effector cells/target cells - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line cell proliferation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are enriched upon KO in C57BL/6 mouse pancreas cells expressing full-length ovalbumin exposed to cytotoxic OT-I CD8 cells at a ratio of 0.25 to 1 BioGRID ORCS Tumor cell survival in the presence of CD8+ T cells
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2195 34529489 pubmed Martin TD (2021) 26-PMID34529489 472 472 Yes 5 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen Pool Cytokine exposure 16 Hours Interferon gamma 20.0 ng - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line protein/peptide accumulation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are enriched in a population showing reduced SIINFEKL presentation upon KO in the presence of IFN-gamma BioGRID ORCS Regulators of SIINFEKL presentation in response to IFN-gamma
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2196 34529489 pubmed Martin TD (2021) 27-PMID34529489 472 472 Yes 3 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen Pool Timecourse 16 Hours - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line protein/peptide accumulation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are enriched in a population showing reduced SIINFEKL presentation upon KO BioGRID ORCS Regulators of SIINFEKL presentation
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2197 34529489 pubmed Martin TD (2021) 28-PMID34529489 472 472 Yes 9 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen Pool Cytokine exposure 16 Hours Interferon gamma 20.0 ng - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line protein/peptide accumulation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are enriched in a population showing reduced HLA presentation upon KO in the presence of interferon-gamma BioGRID ORCS Regulators of HLA presentation in response to IFN-gamma
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2198 34529489 pubmed Martin TD (2021) 29-PMID34529489 472 472 Yes 40 MaGeCK Score Significance Score.3 (FDR) < 0.1 High Throughput Phenotype Screen Pool Timecourse 16 Hours - - - Mouse Tumor Suppressor Gene (TSG) targeted library (Elledge, 2021) CRISPRn Knockout Cas9 C57BL/6 mouse pancreas cells expressing full-length ovalbumin Pancreatic Cell Line protein/peptide accumulation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus Hit genes are enriched in a population showing reduced HLA presentation upon KO BioGRID ORCS Regulators of HLA presentation
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2211 36794958 pubmed Gilliland HN (2023) 1-PMID36794958 11702 11702 Yes 100 Robust-rank aggregation (RRA) Score Significance Score.1 (Rank) <= 100.0 High Throughput Phenotype Screen Pool Bacteria Exposure 4 Hours Mycobacterium abscessus 5.0 MOI - Brie (mouse) CRISPRn Knockout Cas9 iBMDM (immortalized bone marrow derived macrophages) macrophage response to bacteria 3 Rank Log2FC p-Value - - 10090 M. musculus Hit genes are enriched in a population sorted for low Mycobacterium abscessus uptake, suggesting KO of the hit gene prevents uptake|Authors used a combination of gene level p-values and sgRNA level log2fc values to determine hits. The top 100 ranked genes from their results met these thresholds. BioGRID ORCS Increased resistance to bacterial uptake
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2307 36396656 pubmed Liu Y (2022) 1-PMID36396656 19674 19674 Yes 1303 MaGeCK Score Significance Score.1 (Beta Score) > 0.643 OR Score.1 (Beta Score) < -0.575 High Throughput Positive and Negative Selection Pool Drug Exposure 12 Days Gemcitabine 20.0 nM - Brie (mouse) CRISPRn Knockout Cas9 4292 KRASG12D/p53R172H Pancreatic Ductal Adenocarcinoma Cell Line response to chemicals 1 Beta Score - - - - 10090 M. musculus Genes with significant positive beta score show increased resistance to gemcitabine upon KO|Genes with significant negative beta score show increased sensitivity to gemcitabine upon KO BioGRID ORCS Increased/decreased drug resistance
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2308 36396656 pubmed Liu Y (2022) 2-PMID36396656 19674 19674 Yes 3483 MaGeCK Score Significance Score.1 (Beta Score) >= 0.1188 OR Score.1 (Beta Score) < -0.1893 High Throughput Positive and Negative Selection Pool Drug Exposure 12 Days selinexor 0.33 µM - Brie (mouse) CRISPRn Knockout Cas9 4292 KRASG12D/p53R172H Pancreatic Ductal Adenocarcinoma Cell Line response to chemicals 1 Beta Score - - - - 10090 M. musculus Genes with significant positive beta score show increased resistance to selinexor upon KO|Genes with significant negative beta score show increased sensitivity to selinexor upon KO BioGRID ORCS Increased/decreased drug resistance
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2359 35804923 pubmed Yang H (2022) 1-PMID35804923 22507 22507 Yes 3 MaGeCK Score Significance Score.1 (MaGeCK Score) > 0.0 AND Score.2 (p-Value) < 0.05 AND Score.3 (FDR) < 0.05 High Throughput Positive Selection Pool Drug Exposure 15 Days Gemcitabine 50.0 nM = 0.3 GECKO v2 CRISPRn Knockout Cas9 TB32047 Pancreatic Cancer Cell Line response to chemicals 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus KO of the hit gene results in increased growth in the presence of gemcitabine BioGRID ORCS Increased drug resistance
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2372 38395437 pubmed Qian Z (2024) 1-PMID38395437 59 59 Yes 9 MaGeCK Score Significance Score.2 (p-Value) < 0.1 High Throughput Phenotype Screen in vivo Implantation to Mouse Model 10 Weeks - - = 0.3 CRISPRa targeted library based on Caprano sgRNAs (Zhao, 2024) CRISPRa Activation dCas9-VP64 BNL CL.2 Liver cell line tumorigenicity 5 MAGeCK pos score p-Value MAGeCK neg score p-Value Log2FC 10090 M. musculus Authors performed a screen to look for genes identified in commonly amplified regions by HBV integrations that induce tumorigenicity in the mouse fetal hepatocyte BNL-CL.2 upon implantation to immune incompetent mice when activated BioGRID ORCS Genes involved in early stage development of Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC)
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2403 36772830 pubmed Al Adhami H (2023) 1-PMID36772830 19673 19673 Yes 126 MaGeCK Score Significance Score.1 (p-Value) < 0.005 High Throughput Phenotype Screen Pool Timecourse 10 Days - - = 0.3 Brie (mouse) CRISPRn Knockout Cas9 Mouse Embryonic Stem Cell Embryonic Cell Line protein/peptide accumulation 2 p-Value Log2FC - - - 10090 M. musculus KO of the hit gene led to increased expression of germline genes suggesting the gene normally functions in silencing of germline gene expression|Authors used a cell line expressing GFP under the control of the endogenous promoter of the germline gene Dazl as a reporter and assayed for increased GFP expression BioGRID ORCS Germline gene silencing factors
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2411 34879274 pubmed Zhao H (2021) 1-PMID34879274 2643 19674 No 2643 Log2 Fold Change (L2FC) All Significant - High Throughput Negative Selection in vivo Implantation to Mouse Model 7 Days - - ~ 0.5 Brie (mouse) CRISPRn Knockout Cas9 OT-I cells Cytotoxic T-lymphocyte (CD8+ T cells) cell proliferation 1 Log2FC - - - - 10090 M. musculus Hit genes represent sgRNAs that are depleted in OT-I cells implanted into wild-type mice for expansion in vivo without lymphodepletion or supplementation of cytokines, suggesting that KO of the gene results in decreased growth and the gene is essential for normal CD8 T cell expansion BioGRID ORCS Cell-essential genes in vivo
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2412 34879274 pubmed Zhao H (2021) 2-PMID34879274 1555 19674 No 1555 Log2 Fold Change (L2FC) All Significant - High Throughput Negative Selection Pool Cytokine exposure 7 Days Interleukin-2 (IL-2) - ~ 0.5 Brie (mouse) CRISPRn Knockout Cas9 OT-I cells Cytotoxic T-lymphocyte (CD8+ T cells) cell proliferation 1 Log2FC - - - - 10090 M. musculus Hit genes represent sgRNAs that are depleted in OT-I cells, suggesting that KO of the gene results in decreased growth and the gene is essential for normal CD8 T cell expansion in vitro BioGRID ORCS Cell-essential genes
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2429 40372436 pubmed Soriaga LB (2025) 2-PMID40372436 21189 21189 Yes 109 MaGeCK Score Significance Score.2 (FDR) < 0.05 OR Score.4 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Virus Exposure 48 Hours Virus: Influenza A (A/Puerto Rico/8/1934(H1N1) 1.0 MOI - Brie (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line response to virus 5 MAGeCK pos score FDR MAGeCK neg score FDR Log2FC 10090 M. musculus Genes with a significant positive score are enriched in a population exposed to the virus suggesting KO of the gene results in increased survival and the gene normally play a pro-viral role|Genes with a significant negative score are depleted in a population exposed to the virus suggesting KO of the gene results in decreased survival and the gene normally play an anti-viral role BioGRID ORCS Increased/decreased resistance to virus
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2469 10.1101/2025.08.26.672429 prepub Fatimah J. Al Khazal (2025) 1-CUSTOM00000028 19648 19648 Yes 2171 MaGeCK Score Significance Score.1 (MAGeCK pos score) < 0.05 AND Score.2 (p-Value) < 0.05 OR Score.4 (MAGeCK neg score) < 0.05 AND Score.5 (p-Value) < 0.05 High Throughput Positive and Negative Selection Pool Timecourse 10 Doublings - - = 0.4 Brie (mouse) CRISPRn Knockout Cas9 imCC Immortal mouse chromaffin cells cell viability 5 MAGeCK pos score p-Value Log2FC MAGeCK neg score p-Value 10090 M. musculus A genome-wide CRISPR screen was carried out in immortalized mouse chromaffin cells to identify potential therapeutic targets for succinate dehydrogenase (SDH)-deficient paraganglioma and pheochromocytoma (PPGL). BioGRID ORCS Cell-essential genes in a Sdhb mutant background
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2474 36476853 pubmed He B (2022) 1-PMID36476853 1146 1146 Yes 59 MaGeCK Score Significance Score.1 (Log2FC) > 0.58 AND Score.2 (p-Value) < 0.05 OR Score.1 (Log2FC) < -0.58 AND Score.2 (p-Value) < 0.05 High Throughput Phenotype Screen Pool Timecourse 7 Days - - = 0.3 MitoCarta2.0 CRISPRn Knockout Cas9 MEF Embryonic Fibroblast Cell Line protein/peptide accumulation 3 Log2FC p-Value FDR - - 10090 M. musculus KO of genes with a significant positive log2foldchange induced expression of a fluorescent protein reporter in place of the ISG15 coding sequence, suggesting that KO of the gene activates the IFN-I response|KO of genes with a significant negative log2foldchange inhibited expression of a fluorescent protein reporter in place of the ISG15 coding sequence, suggesting that KO of the gene inhibits the IFN-I response BioGRID ORCS Regulation of the IFN-I response
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2477 37059858 pubmed Ruan Y (2023) 1-PMID37059858 19674 19674 Yes 4342 MaGeCK Score Significance Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 High Throughput Positive and Negative Selection Pool Other 14 Days Leukaemia inhibitory factors (LIF)/serum condition (L/S) - = 0.4 Brie (mouse) CRISPRn Knockout Cas9 R1 Embryonic Stem Cell Line cell proliferation 5 MAGeCK neg score p-Value MAGeCK pos score p-Value Log2FC 10090 M. musculus Genes whose sgRNAs are significantly depleted (negative MAGeCK score) are required for mESC fitness|Genes whose sgRNAs were enriched (positive MAGeCK score) inhibit the self-renewal of mESCs BioGRID ORCS Regulation of embryonic stem cell renewal
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2488 39681701 pubmed Uijttewaal ECH (2024) 1-PMID39681701 20461 20461 Yes 477 CRISPR-StAR–MAGeCK analysis pipeline Score Significance Score.1 (Log2FC) < -3.0 AND Score.2 (RRA score) >= 10.0 High Throughput Negative Selection Pool Timecourse 14 Days - - = 0.25 CRISPRn library in CRISPR-StAR vector (Elling, 2024) CRISPRn Knockout Cas9 YUMM1.7 Melanoma Cell Line cell proliferation 2 Log2FC RRA score - - - 10090 M. musculus The authors screened for genes whose KO resulted in decreased proliferation in the Yumm1.7 450R mouse melanoma cell line using CRISPR-StAR BioGRID ORCS Cell-essential genes
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2490 39681701 pubmed Uijttewaal ECH (2024) 3-PMID39681701 538 538 Yes 29 CRISPR-StAR–MAGeCK analysis pipeline Score Significance Score.1 (Log2FC) < -1.5 OR Score.1 (Log2FC) > 1.0 High Throughput Positive and Negative Selection Pool Drug Exposure 14 Days dabrafenib 100.0 nM = 0.25 Targeted CRISPRn library in CRISPR-StAR vector (Elling, 2024) CRISPRn Knockout Cas9 YUMM1.7 Melanoma Cell Line response to chemicals 3 Log2FC RRA score UMI - - 10090 M. musculus Hit genes show increased (negative log2fc) or decreased (positive log2fc) sensitivity to dabrafenib upon KO BioGRID ORCS Increased/decreased drug resistance
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2491 39681701 pubmed Uijttewaal ECH (2024) 4-PMID39681701 538 538 Yes 19 CRISPR-StAR–MAGeCK analysis pipeline Score Significance Score.1 (Log2FC) < -1.5 OR Score.1 (Log2FC) > 1.0 High Throughput Positive and Negative Selection Pool Timecourse 14 Days - - = 0.25 Targeted CRISPRn library in CRISPR-StAR vector (Elling, 2024) CRISPRn Knockout Cas9 YUMM1.7 Melanoma Cell Line cell proliferation 2 Log2FC RRA score - - - 10090 M. musculus Hit genes show increased (positive log2fc) or decreased (negative log2fc) growth upon KO in vitro BioGRID ORCS Cell-essential and growth restrictive genes
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2492 39681701 pubmed Uijttewaal ECH (2024) 2-PMID39681701 20461 20461 Yes 515 CRISPR-StAR–MAGeCK analysis pipeline Column Significance - High Throughput Positive and Negative Selection Pool Implantation to Mouse Model 14 Days - - = 0.25 CRISPRn library in CRISPR-StAR vector (Elling, 2024) CRISPRn Knockout Cas9 YUMM1.7 Melanoma Cell Line cell proliferation 3 Log2FC RRA score UMI - - 10090 M. musculus The authors screened for genes whose KO resulted in decreased (negative log2fc) or increased (positive log2fc) proliferation in established tumors from Yumm1.7 450R mouse melanoma cells injected into Rag2-/- mouse models using CRISPR-StAR.|To select for genes whose dependency is specific for in vivo conditions genes with an in vitro log2fc <-1.5 have been filtered out from the list of hits BioGRID ORCS Cell-essential and growth restrictive genes in established tumors in vivo
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2493 39681701 pubmed Uijttewaal ECH (2024) 5-PMID39681701 538 538 Yes 139 CRISPR-StAR–MAGeCK analysis pipeline Score Significance Score.1 (Log2FC) < -1.5 OR Score.1 (Log2FC) > 1.0 High Throughput Positive and Negative Selection Pool Implantation to Mouse Model 14 Days - - = 0.25 Targeted CRISPRn library in CRISPR-StAR vector (Elling, 2024) CRISPRn Knockout Cas9 YUMM1.7 Melanoma Cell Line cell proliferation 2 Log2FC RRA score - - - 10090 M. musculus The authors screened for genes whose KO resulted in decreased (negative log2fc) or increased (positive log2fc) proliferation in established tumors from Yumm1.7 450R mouse melanoma cells injected into Rag2-/- mouse models using CRISPR-StAR BioGRID ORCS Cell-essential and growth restrictive genes in established tumors in vivo
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