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1270 32968282 pubmed Lawson KA (2020) 12-PMID32968282 19466 19466 Yes 2060 BAGEL Score Significance Score.1 (Bayes Factor) > 5.0 High Throughput Negative Selection Pool Timecourse 17 Days - - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 B16-MO4 Melanoma Cell Line cell proliferation 1 Bayes Factor - - - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
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1271 32968282 pubmed Lawson KA (2020) 13-PMID32968282 18680 18680 Yes 332 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 15 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1272 32968282 pubmed Lawson KA (2020) 14-PMID32968282 18983 18983 Yes 471 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 24 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1273 32968282 pubmed Lawson KA (2020) 15-PMID32968282 19041 19041 Yes 780 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 11 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 EMT6 Mammary Gland Tumor Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1274 32968282 pubmed Lawson KA (2020) 16-PMID32968282 18986 18986 Yes 624 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 19 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 EMT6 Mammary Gland Tumor Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1275 32968282 pubmed Lawson KA (2020) 17-PMID32968282 19060 19060 Yes 493 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 11 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1276 32968282 pubmed Lawson KA (2020) 18-PMID32968282 19032 19032 Yes 451 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 17 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 CT26 Colonic Cancer Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1277 32968282 pubmed Lawson KA (2020) 19-PMID32968282 19011 19011 Yes 243 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 14 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1278 32968282 pubmed Lawson KA (2020) 20-PMID32968282 18625 18625 Yes 167 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 25 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1279 32968282 pubmed Lawson KA (2020) 21-PMID32968282 19046 19046 Yes 125 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 12 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 MC38-OVA Colonic Adenocarcinoma Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1280 32968282 pubmed Lawson KA (2020) 22-PMID32968282 18638 18638 Yes 44 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 15 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 MC38-OVA Colonic Adenocarcinoma Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1281 32968282 pubmed Lawson KA (2020) 23-PMID32968282 19065 19065 Yes 119 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 13 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 B16-MO4 Melanoma Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1282 32968282 pubmed Lawson KA (2020) 24-PMID32968282 19060 19060 Yes 116 DrugZ Score Significance Score.2 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool T cell exposure 17 Days cytotoxic T lymphocytes (CTLs) - ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 B16-MO4 Melanoma Cell Line cell proliferation 2 Z-score FDR - - - 10090 M. musculus Genes with a positive z-score confer resistance to cytotoxic T lymphocytes upon KO. Genes with a negative score confer sensitivity to cytotoxic T lymphocytes upon KO. BioGRID ORCS Increased or decreased cancer cell sensitivity to activated cytotoxic T lymphocytes
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1283 32968282 pubmed Lawson KA (2020) 25-PMID32968282 19063 19063 Yes 139 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 15 Days Interferon gamma 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to IFN-gamma upon KO, genes with a negative Z-score confer reduced proliferation in response to IFN-gamma upon KO BioGRID ORCS Response to IFNgamma
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1284 32968282 pubmed Lawson KA (2020) 26-PMID32968282 19045 19045 Yes 236 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 24 Days Interferon gamma 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to IFN-gamma upon KO, genes with a negative Z-score confer reduced proliferation in response to IFN-gamma upon KO BioGRID ORCS Response to IFNgamma
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1285 32968282 pubmed Lawson KA (2020) 27-PMID32968282 19059 19059 Yes 931 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 15 Days Mutation: FITM2 (ETG228859) KO|IFNgamma 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to IFN-gamma upon KO, genes with a negative Z-score confer reduced proliferation in response to IFN-gamma upon KO BioGRID ORCS Response to IFNgamma in a Fitm2 mutant background
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1286 32968282 pubmed Lawson KA (2020) 28-PMID32968282 18972 18972 Yes 513 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 24 Days Mutation: FITM2 (ETG228859) KO|IFNgamma 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to IFN-gamma upon KO, genes with a negative Z-score confer reduced proliferation in response to IFN-gamma upon KO BioGRID ORCS Response to IFNgamma in a Fitm2 mutant background
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1287 32968282 pubmed Lawson KA (2020) 29-PMID32968282 19064 19064 Yes 259 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 15 Days TNF‐α 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to TNF upon KO, genes with a negative Z-score confer reduced proliferation in response to TNF upon KO BioGRID ORCS Response to TNF
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1288 32968282 pubmed Lawson KA (2020) 30-PMID32968282 19050 19050 Yes 214 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 24 Days TNF‐α 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to TNF upon KO, genes with a negative Z-score confer reduced proliferation in response to TNF upon KO BioGRID ORCS Response to TNF
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1289 32968282 pubmed Lawson KA (2020) 31-PMID32968282 19058 19058 Yes 904 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 15 Days Mutation: ATG12 (ETG67526) KO | TNF 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to TNF upon KO, genes with a negative Z-score confer reduced proliferation in response to TNF upon KO BioGRID ORCS Response to TNF in an ATG12 mutant background
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1290 32968282 pubmed Lawson KA (2020) 32-PMID32968282 19032 19032 Yes 924 DrugZ Score Significance Score.3 (FDR) < 0.05 OR Score.5 (FDR) < 0.05 High Throughput Positive and Negative Selection Pool Cytokine exposure 24 Days Mutation: ATG12 (ETG67526) KO | TNF 10.0 ng/mL ~ 0.5 mTKO (mouseTKO) CRISPRn Knockout Cas9 RenCa Mouse kidney carcinoma cell cell proliferation 5 Z-score p-Value FDR p-Value FDR 10090 M. musculus Genes with a positive Z-score confer increased proliferation in response to TNF upon KO, genes with a negative Z-score confer reduced proliferation in response to TNF upon KO BioGRID ORCS Response to TNF in an ATG12 mutant background
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1358 31346084 pubmed Orvedahl A (2019) 1-PMID31346084 1536 20147 No 14 STARS Score Significance Score.1 (STARS Score) > 0.0 AND Score.2 (FDR) < 0.1 High Throughput Positive Selection Pool Ligand Exposure 7 Days Interferon gamma 10.0 units/mL - Brie (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line cell proliferation 2 STARS Score FDR - - - 10090 M. musculus This CRISPR screen identified genes that showed resistance to interferon gamma (STARS analysis at FDR < 10%) and none that showed increased sensitivity.|The authors note the following: p-value=0 means <5.97728631202e-06, FDR=0 means <0.00131158739647 BioGRID ORCS Increased ligand resistance
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1359 31346084 pubmed Orvedahl A (2019) 2-PMID31346084 1700 20147 No 9 STARS Score Significance Score.1 (STARS Score) > 0.0 AND Score.2 (FDR) < 0.1 High Throughput Positive Selection Pool Ligand Exposure 7 Days Interferon gamma 10.0 units/mL - Brie (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line cell proliferation 2 STARS Score FDR - - - 10090 M. musculus This genome wide genetic interaction CRISPR screen involved using Atg5 knockout (Atg5KO) cells with the purpose of identifying genes required for the hypersensitivity of Atg5KO cells to interferon gamma-induced cell death.|The identified genes showed resistance to interferon gamma (STARS analysis at FDR < 10%) and none showed increased sensitivity.|The authors note the following: p-value=0 means <5.97728631202e-06, FDR=0 means <0.0012701733413 BioGRID ORCS Increased ligand resistance
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1398 31038839 pubmed Kim B (2019) 1-PMID31038839 19672 19672 Yes 10 MaGeCK Score Significance Score.2 (p-Value) < 0.0005 High Throughput Phenotype Screen Pool Timecourse 8 Doublings - - - Brie (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line protein/peptide accumulation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus CDr20- cell populations indicate KO of the gene results in loss of microglia as measured by the CDr20 fluorescent reporter BioGRID ORCS Microglia essential genes
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1399 31038839 pubmed Kim B (2019) 2-PMID31038839 19672 19672 Yes 10 MaGeCK Score Significance Score.2 (p-Value) < 0.0005 High Throughput Phenotype Screen Pool Timecourse 9 Doublings - - - Brie (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line protein/peptide accumulation 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus CDr20- cell populations indicate KO of the gene results in loss of microglia as measured by the CDr20 fluorescent reporter BioGRID ORCS Microglia essential genes
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1422 33208938 pubmed Ma H (2020) 1-PMID33208938 22468 22468 Yes 56 MaGeCK Score Significance Score.2 (p-Value) < 0.01 High Throughput Phenotype Screen Pool Virus Exposure 54 Hours Virus: Venezuelan equine encephalitis virus (VEEV) based chimera (SINV–VEEV–GFP) 1 MOI ~ 0.1 GECKO v2 (mouse) CRISPRn Knockout Cas9 Neuro-2a Neuroblastoma Cell Line response to virus 4 MaGeCK Score p-Value FDR Log2FC - 10090 M. musculus KO of the hit genes prevents infection by Venezuelan equine encephalitis virus as measured by expression of GFP after inoculation with a chimeric SINV-VEEV-GFP that encodes GFP BioGRID ORCS Increased resistance to virus
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1438 33087464 pubmed Urbanek K (2020) 1-PMID33087464 5382 20077 No 895 STARS Score Significance Score.3 (FDR) < 0.25 High Throughput Positive Selection Pool Virus Exposure 9 Days Virus: Reovirus (strain T3SA+) 100.0 MOI = 0.2 Asagio (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line response to virus 3 STARS Score p-Value FDR - - 10090 M. musculus Hit genes represent host factors required for reovirus-induced cell killing BioGRID ORCS Increased resistance to virus
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1439 33087464 pubmed Urbanek K (2020) 2-PMID33087464 6736 20077 No 52 STARS Score Significance Score.3 (FDR) < 0.25 High Throughput Positive Selection Pool Virus Exposure 9 Days Virus: Reovirus (strain T3SA-) 100.0 MOI = 0.2 Asagio (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line response to virus 3 STARS Score p-Value FDR - - 10090 M. musculus Hit genes represent host factors required for reovirus-induced cell killing BioGRID ORCS Increased resistance to virus
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1440 31209294 pubmed Fu X (2019) 2-PMID31209294 20646 20646 Yes 477 MaGeCK Score Significance Score.2 (neg score p-value) < 0.01 OR Score.4 (pos score p-value) < 0.01 High Throughput Phenotype Screen Pool Other 1 Days Dux gene induction - - Brie (mouse) CRISPRn Knockout Cas9 Mouse Embryonic Stem Cell Embryonic Cell Line protein/peptide accumulation 5 MAGeCK neg score neg score p-value MAGeCK pos score pos score p-value Log2FC 10090 M. musculus Genes whose KO results in a negative MAGeCK score associated with a significant p-value are depleted in the totipotent population, suggesting they are positive regulators of totipotency.|Genes whose KO results in a positive MAGeCK score associated with a significant p-value are enriched in the totipotent population, suggesting they are negative regulators of totipotency. BioGRID ORCS Regulators of totipotency
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1460 33157015 pubmed Bieging-Rolett KT (2020) 1-PMID33157015 89 89 Yes 11 CasTLE Score Significance Score.1 (CasTLE Effect) > 0.0 AND Score.2 (p-Value) < 0.05 High Throughput Positive Selection in vivo Implantation to Mouse Model 3 Weeks Mutation: HrasG12V (ETG15461) - = 0.2 TSAG library CRISPRn Knockout Cas9 MEF Embryonic Fibroblast Cell Line tumorigenicity 2 CasTLE Effect p-Value - - - 10090 M. musculus KO of the hit genes causes increased growth in an E1A;HrasG12V,p53 tumor environment, suggesting that normal function of the gene is tumor suppressive BioGRID ORCS Tumor suppressor genes
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1468 33185187 pubmed Fomicheva M (2020) 1-PMID33185187 22619 22619 Yes 6 MaGeCK Score Significance Score.3 (FDR) < 0.25 High Throughput Phenotype Screen Pool Timecourse 14 Days - - = 0.3 GECKO v2 (mouse) CRISPRn Knockout Cas9 EpH4 breast epithelium cell cycle progression 3 MaGeCK Score p-Value FDR - - 10090 M. musculus Authors screened for genes whose KO resulted in proliferation despite high cell density, suggesting the gene normally contributes to density-dependent cell cycle arrest BioGRID ORCS Positive regulators of density-dependent cell cycle arrest
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1469 33185187 pubmed Fomicheva M (2020) 2-PMID33185187 22619 22619 Yes 4 MaGeCK Score Significance Score.3 (FDR) < 0.25 High Throughput Phenotype Screen Pool Timecourse 28 Days - - = 0.3 GECKO v2 (mouse) CRISPRn Knockout Cas9 EpH4 breast epithelium cell cycle progression 3 MaGeCK Score p-Value FDR - - 10090 M. musculus Authors screened for genes whose KO resulted in proliferation despite high cell density, suggesting the gene normally contributes to density-dependent cell cycle arrest BioGRID ORCS Positive regulators of density-dependent cell cycle arrest
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1470 33185187 pubmed Fomicheva M (2020) 3-PMID33185187 22619 22619 Yes 6 MaGeCK Score Significance Score.3 (FDR) < 0.25 High Throughput Positive Selection Pool Timecourse 42 Days - - = 0.3 GECKO v2 (mouse) CRISPRn Knockout Cas9 EpH4 breast epithelium cell cycle progression 3 MaGeCK Score p-Value FDR - - 10090 M. musculus Authors screened for genes whose KO resulted in proliferation despite high cell density, suggesting the gene normally contributes to density-dependent cell cycle arrest BioGRID ORCS Positive regulators of density-dependent cell cycle arrest
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1481 32640256 pubmed Loo CS (2020) 1-PMID32640256 19668 19668 Yes 490 MaGeCK Score Significance Score.2 (p-Value) < 0.01 AND Score.3 (Log2FC) < -0.5 High Throughput Phenotype Screen Pool Timecourse 6 Days - - < 0.2 Brie (mouse) CRISPRn Knockout Cas9 primary Treg Regulatory T cell protein/peptide accumulation 3 MAGeCK neg score p-Value Log2FC - - 10090 M. musculus - BioGRID ORCS Negative regulators of Foxp3
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1482 32640256 pubmed Loo CS (2020) 2-PMID32640256 19668 19668 Yes 254 MaGeCK Score Significance Score.2 (p-Value) < 0.01 AND Score.3 (Log2FC) > 0.5 High Throughput Phenotype Screen Pool Timecourse 6 Days - - < 0.2 Brie (mouse) CRISPRn Knockout Cas9 primary Treg Regulatory T cell protein/peptide accumulation 3 MAGeCK pos score p-Value Log2FC - - 10090 M. musculus - BioGRID ORCS Positive regulators of Foxp3
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1483 32640256 pubmed Loo CS (2020) 3-PMID32640256 19674 19674 Yes 1497 MaGeCK Score Significance Score.2 (p-Value) < 0.002 AND Score.3 (Log2FC) < -1.0 High Throughput Negative Selection Pool Timecourse 6 Days - - < 0.2 Brie (mouse) CRISPRn Knockout Cas9 primary Treg Regulatory T cell cell proliferation 3 MaGeCK Score p-Value Log2FC - - 10090 M. musculus - BioGRID ORCS Cell-essential genes
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1484 32640256 pubmed Loo CS (2020) 4-PMID32640256 19674 19674 Yes 22 MaGeCK Score Significance Score.2 (p-Value) < 0.002 AND Score.3 (Log2FC) >= 0.65567 High Throughput Positive Selection Pool Timecourse 6 Days - - < 0.2 Brie (mouse) CRISPRn Knockout Cas9 primary Treg Regulatory T cell cell proliferation 3 MaGeCK Score p-Value Log2FC - - 10090 M. musculus - BioGRID ORCS Growth restrictive genes
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1489 32156810 pubmed Ma H (2020) 1-PMID32156810 200 20077 No 200 MaGeCK All Significant - High Throughput Phenotype Screen Pool Cytokine exposure 16 Hours IFN-β (Interferon beta) 40.0 IU/ml - Asagio (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line protein/peptide accumulation 3 MaGeCK Score p-Value FDR - - 10090 M. musculus To screen for negative regulators of the IFN pathway the authors used Bst2 (tetherin), a cell surface protein induced by type I IFNs, to select cells with high IFN pathway activity and then looked at sgRNAs enriched in this population suggesting that KO of the hit gene resulted in increased IFN pathway activity BioGRID ORCS Negative regulators of the IFN pathway
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1490 32156810 pubmed Ma H (2020) 2-PMID32156810 200 20077 No 200 MaGeCK All Significant - High Throughput Phenotype Screen Pool Cytokine exposure 16 Hours IFN-β (Interferon beta) 40.0 IU/ml - Asagio (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line protein/peptide accumulation 3 MaGeCK Score p-Value FDR - - 10090 M. musculus To screen for negative regulators of the IFN pathway the authors used Bst2 (tetherin), a cell surface protein induced by type I IFNs, to select cells with high IFN pathway activity and then looked at sgRNAs enriched in this population suggesting that KO of the hit gene resulted in increased IFN pathway activity BioGRID ORCS Negative regulators of the IFN pathway
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1521 31657721 pubmed Kinnebrew M (2019) 1-PMID31657721 1239 1239 Yes 253 MaGeCK Score Significance Score.1 (p-Value) < 0.1 High Throughput Phenotype Screen Pool Ligand Exposure 24 Hours Sonic hedgehog protein (mouse) 3.2 nM - Targeted lipid-related genes library (Rohatgi, 2019) CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line protein/peptide accumulation 3 p-Value FDR Log2FC - - 10090 M. musculus From the paper: we treated cells with a low, sub-saturating concentration of SHH (LoSHH) that activated the reporter to <10% of maximal strength and selected super-responders, cells with the top 5% of GLI-GFP fluorescence BioGRID ORCS Negative regulators of the HH pathway
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1522 31657721 pubmed Kinnebrew M (2019) 2-PMID31657721 1239 1239 Yes 190 MaGeCK Score Significance Score.1 (p-Value) < 0.1 High Throughput Phenotype Screen Pool Ligand Exposure 24 Hours Sonic hedgehog protein (mouse) 25 nM - Targeted lipid-related genes library (Rohatgi, 2019) CRISPRn Knockout Cas9 NIH 3T3 Embryonic Fibroblast Cell Line protein/peptide accumulation 3 p-Value FDR Log2FC - - 10090 M. musculus From the paper: "we treated cells with a high, saturating concentration of the ligand Sonic Hedgehog (HiSHH) and used Fluorescence Activated Cell Sorting (FACS) to collect poor responders, those with the lowest 10% of GLI-GFP fluorescence" BioGRID ORCS Positive regulators of the HH pathway
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1523 32355287 pubmed Liu X (2020) 1-PMID32355287 566 566 Yes 27 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days 6-Mercaptopurine 0.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1525 32355287 pubmed Liu X (2020) 2-PMID32355287 566 566 Yes 51 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days A-966492 2.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1526 32355287 pubmed Liu X (2020) 3-PMID32355287 566 566 Yes 43 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days ABT-888 5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1527 32355287 pubmed Liu X (2020) 4-PMID32355287 566 566 Yes 26 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Amonafide 0.25 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1528 32355287 pubmed Liu X (2020) 5-PMID32355287 566 566 Yes 32 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Amsacrine 25.84 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1529 32355287 pubmed Liu X (2020) 6-PMID32355287 566 566 Yes 30 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days 5-Azacytidine 0.125 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1530 32355287 pubmed Liu X (2020) 7-PMID32355287 566 566 Yes 56 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days AZD-2461 2.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1531 32355287 pubmed Liu X (2020) 8-PMID32355287 566 566 Yes 29 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days BMH-21 100 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
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1532 32355287 pubmed Liu X (2020) 9-PMID32355287 566 566 Yes 21 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Cytarabine 100 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1533 32355287 pubmed Liu X (2020) 10-PMID32355287 566 566 Yes 41 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Dacarbazine 0.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1534 32355287 pubmed Liu X (2020) 11-PMID32355287 566 566 Yes 52 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Daunorubicin 31.6 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1535 32355287 pubmed Liu X (2020) 12-PMID32355287 566 566 Yes 36 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days DR 2313 9.146 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1536 32355287 pubmed Liu X (2020) 13-PMID32355287 566 566 Yes 48 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Ellipticine 0.147 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1537 32355287 pubmed Liu X (2020) 14-PMID32355287 566 566 Yes 55 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Epirubicin 0.192 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1538 32355287 pubmed Liu X (2020) 15-PMID32355287 566 566 Yes 43 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Etoposide 25 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1539 32355287 pubmed Liu X (2020) 16-PMID32355287 566 566 Yes 17 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days EX-527 2.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1540 32355287 pubmed Liu X (2020) 17-PMID32355287 566 566 Yes 57 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Hydroxycamptothecin 13.06 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1541 32355287 pubmed Liu X (2020) 18-PMID32355287 566 566 Yes 1 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Hydroxyurea 25 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1542 32355287 pubmed Liu X (2020) 19-PMID32355287 566 566 Yes 66 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Irinotecan 1.25 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1543 32355287 pubmed Liu X (2020) 20-PMID32355287 566 566 Yes 52 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days KU-57788 0.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1544 32355287 pubmed Liu X (2020) 21-PMID32355287 566 566 Yes 39 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days KU-60019 1 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1545 32355287 pubmed Liu X (2020) 22-PMID32355287 566 566 Yes 55 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Melphalan 1 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1546 32355287 pubmed Liu X (2020) 23-PMID32355287 566 566 Yes 63 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Mitomycin C 25 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1547 32355287 pubmed Liu X (2020) 24-PMID32355287 566 566 Yes 50 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days MK-4827 0.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1548 32355287 pubmed Liu X (2020) 25-PMID32355287 566 566 Yes 59 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Methyl methanesulfonate (MMS) 50 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1549 32355287 pubmed Liu X (2020) 26-PMID32355287 566 566 Yes 58 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Olaparib 1.25 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1550 32355287 pubmed Liu X (2020) 27-PMID32355287 566 566 Yes 48 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Oxaliplatin 0.25 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1551 32355287 pubmed Liu X (2020) 28-PMID32355287 566 566 Yes 64 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Pirarubicin 50 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1552 32355287 pubmed Liu X (2020) 29-PMID32355287 566 566 Yes 36 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Pyridostatin 0.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1553 32355287 pubmed Liu X (2020) 30-PMID32355287 566 566 Yes 49 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Rucaparib 1.25 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1554 32355287 pubmed Liu X (2020) 31-PMID32355287 566 566 Yes 60 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days SN-38 10 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1555 32355287 pubmed Liu X (2020) 32-PMID32355287 566 566 Yes 22 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days SRT 1720 2.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1556 32355287 pubmed Liu X (2020) 33-PMID32355287 566 566 Yes 49 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Thio-TEPA 1 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1557 32355287 pubmed Liu X (2020) 34-PMID32355287 566 566 Yes 56 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Topotecan 25 nM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1558 32355287 pubmed Liu X (2020) 35-PMID32355287 566 566 Yes 65 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Treosulfan 2.5 µM - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1559 32355287 pubmed Liu X (2020) 36-PMID32355287 566 566 Yes 74 MaGeCK Score Significance Score.1 (p-Value) < 0.05 AND Score.2 (FDR) < 0.05 Low Throughput Negative Selection Pool Drug Exposure 12 Days Zeocin 2.5 µg/mL - CRISPRn targeted mouse library (Liu, 2020) CRISPRn Knockout Cas9 CH12F3 Lymphoma Cell Line response to chemicals 3 p-Value FDR MAGeCK neg score - - 10090 M. musculus The authors combined chemical screens using 36 DNA-damaging compounds with targeted CRISPR knockout screens focused on 414 DNA repair genes in the mouse CH12F3 lymphoma cell line. BioGRID ORCS Decreased resistance to drug
|
1565 33676427 pubmed Du R (2021) 1-PMID33676427 19674 19674 Yes 1943 MaGeCK Score Significance Score.3 (FDR) < 0.05 High Throughput Negative Selection Pool Drug Exposure 12 Days salirasib (Farnesyl Thiosalicylic Acid) 100.0 µM - Brie (mouse) CRISPRn Knockout Cas9 4292 KRASG12D/p53R172H Pancreatic Ductal Adenocarcinoma Cell Line response to chemicals 3 MaGeCK Score p-Value FDR - - 10090 M. musculus KO of hit genes with a negative beta score causes decreased proliferation in the presence of FTS, KO of hit genes with a positive beta score causes increased proliferation in the presence of FTS BioGRID ORCS Increased/decreased drug resistance
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1570 32499641 pubmed Cortez JT (2020) 1-PMID32499641 493 493 Yes 41 MaGeCK Score Significance Score.2 (FDR) < 0.5 OR Score.4 (FDR) < 0.5 High Throughput Phenotype Screen Pool Timecourse 8 Days - - - CRISPRn Nuclear Factor Targeted Library based on Brie sgRNAs CRISPRn Knockout Cas9 primary Treg Regulatory T cell protein/peptide accumulation 5 MAGeCK neg score FDR MAGeCK pos score FDR Log2FC 10090 M. musculus Authors screened mouse primary Treg cells using a targeted library of ~490 nuclear factors based on guides from the Brie library. They sorted cells using a Foxp3 fluorescent reporter for cells with low Foxp3 expression.|Genes whose KO resulted in enrichment in a low Foxp3 population (significant pos MAGECK score) suggest the gene normally functions as a positive regulator of Foxp3 levels|Genes whose KO resulted in depletion in a low Foxp3 population (significant neg MAGECK score) suggest the gene normally functions as a negative regulator of Foxp3 levels BioGRID ORCS Regulators of Foxp3 stability
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1585 33372156 pubmed Balce DR (2021) 1-PMID33372156 17448 17448 Yes 229 Log2 Fold Change (L2FC) Score Significance Score.1 (Log2FC) > 0.0 AND Score.2 (p-Value) > 1.3 High Throughput Phenotype Screen Pool Cytokine exposure 20 Hours Interferon gamma 2.0 units/mL - Asagio (mouse) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line protein/peptide distribution 2 Log2FC p-Value - - - 10090 M. musculus authors screened for genes whose KO resulted in increased levels of iNOS (a classic marker for macrophage activation) suggesting the gene normally acts as a negative regulator of IFNgamma responses BioGRID ORCS Negative regulators of the IFN pathway
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1587 30944478 pubmed Pluvinage JV (2019) 1-PMID30944478 2967 2967 Yes 286 CasTLE Score Significance Score.2 (p-Value) < 0.05 High Throughput Phenotype Screen Pool Drug Exposure 7 Days - - < 1 Bassik Mouse CRISPR Knockout Library: Drug targets, kinases, phosphatases and Membrane Proteins sub-libraries CRISPRn Knockout Cas9 BV-2 Microglial Cell Line phagocytosis 2 CasTLE Score p-Value - - - 10090 M. musculus - BioGRID ORCS Regulators of phagocytosis
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1662 34290256 pubmed VanDusen NJ (2021) 1-PMID34290256 2444 2444 Yes 262 MaGeCK Score Significance Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 High Throughput Phenotype Screen in vivo Timecourse 4 Weeks - - - Murine Transcriptional Regulator Library (Pu, 2021) CRISPRn Knockout Cas9 cardiomyocyte cell line cardiac muscle cell line protein/peptide accumulation 5 MAGeCK pos score p-Value MAGeCK neg score p-Value Log2FC 10090 M. musculus Myh7-YFP is being used as a reporter for cardiomyocyte (CM) maturation|Authors used CRISPR/Cas9 AAV9 (CASAAV)-based somatic mutagenesis|Genes with a significant positive score (p<0.05) represent genes whose KO results in enrichment in a Myh7-YFP high population, suggesting that KO of the gene results in decreased CM maturation and the gene normally promotes CM maturation|Genes with a significant negative score (p<0.05) represent genes whose KO results in the depletion in a Myh7-YFP high population, suggesting KO of the gene results in increased CM maturation and the gene normally inhibits CM maturation BioGRID ORCS Genes involved in regulation of cardiomyocyte maturation
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1663 34582788 pubmed Wang X (2021) 1-PMID34582788 4721 4721 Yes 265 MaGeCK Score Significance Score.2 (p-Value) < 0.001 OR Score.4 (p-Value) < 0.001 High Throughput Positive and Negative Selection Pool Timecourse 10 Passages - - ~ 0.3 MusCK CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line cell proliferation 5 MAGeCK neg score p-Value MAGeCK pos score p-Value Log2FC 10090 M. musculus Authors screened a library of over 4,500 genes implicated in tumor initiation, progression, and immune modulation for essentiality in a breast cancer cell line BioGRID ORCS Cell-essential genes and growth restrictive genes
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1664 34582788 pubmed Wang X (2021) 2-PMID34582788 4776 4776 Yes 12 MaGeCK Score Significance Score.2 (p-Value) < 0.001 OR Score.4 (p-Value) < 0.001 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model 16 Days BALB/c VS. BALB/c Foxn1nu/nu hosts - ~ 0.3 MusCK CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 MAGeCK neg score p-Value MAGeCK pos score p-Value Log2FC 10090 M. musculus Authors inspected sgRNAs depleted from tumors in wild-type immunocompetent hosts compared to nude immunodeficient mice to reveal key genes promoting immune evasion in 4T1 cancer cells|Significant negative score indicates depletion of sgRNA in wt compared to immuno-deficent host, significant positive score indicates enrichment of sgRNA in wt compared to immuno-deficient host BioGRID ORCS Regulation of cancer cell response to immune system
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1666 34582788 pubmed Wang X (2021) 3-PMID34582788 83 83 Yes 6 MaGeCK Score Significance Score.2 (p-Value) < 0.001 OR Score.4 (p-Value) < 0.001 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model 16 Days BALB/c VS. BALB/c Foxn1nu/nu hosts - ~ 0.3 MusCK 2.0 CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 MAGeCK neg score p-Value MAGeCK pos score p-Value Log2FC 10090 M. musculus Analysis of sgRNA in wildtype vs. immune-deficient hosts|Significant negative score indicates depletion of sgRNA in wt compared to immuno-deficent host BioGRID ORCS Regulation of cancer cell response to immune system
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1667 34582788 pubmed Wang X (2021) 4-PMID34582788 83 83 Yes 9 MaGeCK Score Significance Score.2 (p-Value) < 0.001 OR Score.4 (p-Value) < 0.001 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model 16 Days BALB/c +OVA VS. BALB/c Foxn1nu/nu hosts - ~ 0.3 MusCK 2.0 CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 MAGeCK neg score p-Value MAGeCK pos score p-Value Log2FC 10090 M. musculus Analysis of sgRNA in wildtype hosts pre-vaccinated with ovalbumin vs. immune-deficient hosts|Significant negative score indicates depletion of sgRNA in wt compared to immuno-deficent host BioGRID ORCS Regulation of cancer cell response to immune system
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1668 34582788 pubmed Wang X (2021) 5-PMID34582788 83 83 Yes 6 MaGeCK Score Significance Score.2 (p-Value) < 0.001 OR Score.4 (p-Value) < 0.001 High Throughput Positive and Negative Selection in vivo Implantation to Mouse Model 16 Days BALB/c +OVA+anti-PD-1 antibody VS. BALB/c Foxn1nu/nu hosts - ~ 0.3 MusCK 2.0 CRISPRn Knockout Cas9 4T1 Breast Adenocarcinoma Cell Line tumorigenicity 5 MAGeCK neg score p-Value MAGeCK pos score p-Value Log2FC 10090 M. musculus Authors inspected sgRNAs depleted from tumors in wild-type immunocompetent hosts, pre-vaccinated with ovalbumin and monoclonal anti-PD-1 treatment compared to nude immunodeficient mice to reveal key genes promoting immune evasion in 4T1 cancer cells and factors that affect antigen-specific T-cell immunity through the PD-1/PD-L1 axis|Significant negative score indicates depletion of sgRNA in wt compared to immuno-deficent host BioGRID ORCS Regulation of cancer cell response to immune system
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1678 31959936 pubmed Marschallinger J (2020) 1-PMID31959936 2807 2807 Yes 219 CasTLE Score Significance Score.3 (p-Value) < 0.05 High Throughput Phenotype Screen Pool Timecourse 90 Hours 5 μg/ml LPS in DMEM with 5% FBS - < 1.0 CRISPRn targeted library (Wyss-Coray, 2020) CRISPRn Knockout Cas9 BV-2 Microglial Cell Line regulation of lipid localization 3 CasTLE Score CasTLE Effect p-Value - - 10090 M. musculus Positive casTLE effect size represents genes targeted by sgRNAs that were enriched in lipid droplet- negative cells; negative effect size represents genes targeted by sgRNAs that were under-represented in lipid droplet- negative cells. BioGRID ORCS Regulators of lipid droplet formation
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1679 31618756 pubmed Hoshino A (2019) 1-PMID31618756 19983 19983 Yes 3198 Delta Z scores Column Significance - High Throughput Phenotype Screen Pool Drug Exposure 10 Days CCCP 13.0 µM ~ 0.5 GECKO v2 (mouse) CRISPRn Knockout Cas9 C2C12 myoblast cell line protein/peptide distribution 2 Z-score Z-score - - - 10090 M. musculus The authors used loss of ectopically expressed matrix GFP protein (Cox8-GFP) as a metric for mitophagy BioGRID ORCS Regulation of mitophagy
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1680 31618756 pubmed Hoshino A (2019) 2-PMID31618756 19983 19983 Yes 3165 Delta Z scores Column Significance - High Throughput Phenotype Screen Pool Drug Exposure 10 Days OAR (1.0 μg/mL Oligomycin, 1.0 μg/mL Antimycin A and 100 nM Rotenone) - ~ 0.5 GECKO v2 (mouse) CRISPRn Knockout Cas9 C2C12 myoblast cell line protein/peptide distribution 2 pos Z-score neg Z-score - - - 10090 M. musculus The authors used loss of ectopically expressed matrix GFP protein (Cox8-GFP) as a metric for mitophagy BioGRID ORCS Regulators of mitophagy
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1681 31618756 pubmed Hoshino A (2019) 3-PMID31618756 19983 19983 Yes 2968 Delta Z scores Column Significance - High Throughput Phenotype Screen Pool Timecourse 10 Days - - ~ 0.5 GECKO v2 (mouse) CRISPRn Knockout Cas9 C2C12 myoblast cell line protein/peptide distribution 2 pos Z-score neg Z-score - - - 10090 M. musculus The authors screened for loss of mitotracker labeling of mitochondrial membrane as a marker for mitophagy BioGRID ORCS Regulators of mitophagy
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1682 31618756 pubmed Hoshino A (2019) 4-PMID31618756 19983 19983 Yes 2684 Delta Z scores Column Significance - High Throughput Phenotype Screen Pool Drug Exposure 10 Days CCCP 13.0 µM ~ 0.5 GECKO v2 (mouse) CRISPRn Knockout Cas9 C2C12 myoblast cell line protein/peptide distribution 2 pos Z-score neg Z-score - - - 10090 M. musculus The authors used altered fluorescence of matrix-targeted mKeima from 440 to 586 as it encounters the low pH environment of the lysosome as a marker for mitophagy BioGRID ORCS Regulators of mitophagy
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1683 31618756 pubmed Hoshino A (2019) 5-PMID31618756 19983 19983 Yes 3008 Delta Z scores Column Significance - High Throughput Phenotype Screen Pool Drug Exposure 10 Days OAR (1.0 μg/mL Oligomycin, 1.0 μg/mL Antimycin A and 100 nM Rotenone) - ~ 0.5 GECKO v2 (mouse) CRISPRn Knockout Cas9 C2C12 myoblast cell line protein/peptide distribution 2 pos Z-score neg Z-score - - - 10090 M. musculus The authors used altered fluorescence of matrix-targeted mKeima from 440 to 586 as it encounters the low pH environment of the lysosome as a marker for mitophagy BioGRID ORCS Regulators of mitophagy
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1684 31618756 pubmed Hoshino A (2019) 6-PMID31618756 19983 19983 Yes 2902 Delta Z scores Column Significance - High Throughput Phenotype Screen Pool Drug Exposure 10 Days CCCP 13.0 µM ~ 0.5 GECKO v2 (mouse) CRISPRn Knockout Cas9 C2C12 myoblast cell line protein/peptide distribution 2 pos Z-score neg Z-score - - - 10090 M. musculus The authors used loss of ectopically expressed outer membrane-targeted GFP (GFP-Omp25) as a marker for mitophagy BioGRID ORCS Regulators of mitophagy
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1685 31618756 pubmed Hoshino A (2019) 7-PMID31618756 19983 19983 Yes 2943 Delta Z scores Column Significance - High Throughput Phenotype Screen Pool Drug Exposure 10 Days OAR (1.0 μg/mL Oligomycin, 1.0 μg/mL Antimycin A and 100 nM Rotenone) - ~ 0.5 GECKO v2 (mouse) CRISPRn Knockout Cas9 C2C12 myoblast cell line protein/peptide distribution 2 pos Z-score neg Z-score - - - 10090 M. musculus The authors used loss of ectopically expressed outer membrane-targeted GFP (GFP-Omp25) as a metric for mitophagy BioGRID ORCS Regulators of mitophagy
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1714 34016692 pubmed Norton JP (2021) 1-PMID34016692 764 20611 No 764 MaGeCK All Significant - High Throughput Negative Selection Pool Timecourse 12 Doublings - - < 1.0 GECKO v2 (mouse) CRISPRn Knockout Cas9 Rb1/Trp53-deleted SCLC mouse small cell lung cancer cell proliferation 3 MaGeCK Score FDR Log2FC - - 10090 M. musculus Authors screened for gene necessary for SCLC cell growth BioGRID ORCS Cell-essential genes
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1716 34016692 pubmed Norton JP (2021) 3-PMID34016692 293 20611 No 293 MaGeCK All Significant - High Throughput Negative Selection Pool Timecourse 12 Doublings - - < 1.0 GECKO v2 (mouse) CRISPRn Knockout Cas9 MEF Embryonic Fibroblast Cell Line cell proliferation 3 MaGeCK Score FDR Log2FC - - 10090 M. musculus Authors screened for genes whose KO resulted in decreased growth of Trp53-null MEFs BioGRID ORCS Cell-essential genes
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1717 34016692 pubmed Norton JP (2021) 4-PMID34016692 23 20611 No 23 MaGeCK All Significant - High Throughput Positive Selection Pool Timecourse 12 Doublings - - < 1.0 GECKO v2 (mouse) CRISPRn Knockout Cas9 MEF Embryonic Fibroblast Cell Line cell proliferation 3 MaGeCK Score FDR Log2FC - - 10090 M. musculus KO of the hit gene causes increased growth BioGRID ORCS Growth restrictive genes
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1720 33562682 pubmed Sanghvi VR (2021) 1-PMID33562682 15829 15829 Yes 17 DESeq Score Significance Score.2 (p-Value) < 0.05 High Throughput Positive Selection Pool Drug Exposure - silvestrol 10.0 nM - mouse CRISPRn library (Yusa, 2013) CRISPRn Knockout Cas9 FL5.12 pro-B-lymphocyte response to chemicals 2 Log2FC p-Value - - - 10090 M. musculus Authors screened for genes whose KO confers resistance to silvestrol BioGRID ORCS Increased drug resistance
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1781 33953401 pubmed Griffin GK (2021) 1-PMID33953401 1057 1057 Yes 155 STARS Score Significance Score.2 (p-Value) < 0.05 High Throughput Negative Selection in vivo Implantation to Mouse Model 15 Days PD-1/CTLA-4 blockade - - CRISPRn Targeted Chromatin Regulator Library (Bernstein, 2021) CRISPRn Knockout Cas9 LL/2 (LLC1) Lung Cancer Cell Line tumorigenicity 3 STARS Score p-Value FDR - - 10090 M. musculus Authors triggered immune checkpoint blockade (ICB) by combination PD-1/CTLA-4 blockade and looked for genes whose KO sensitized (decreased proliferation) of tumor cells in response to ICB. BioGRID ORCS Tumor cell response to immune checkpoint blockade
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