PMCID string | Title string | Sentences string |
|---|---|---|
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Once cells reached confluence, perpendicular scratches were carefully made across the marked lines. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Subsequently, PBS was employed to remove any cellular debris, and the cells were cultured in McCoy’s 5 A medium with low serum at 37 ℃ and 5% CO2.The widths of the scratches were observed and documented at 0-, 24-, and 48- hours post-scratching using an inverted biological microscope (CX53, Olympus, Japan). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Three fields of view were captured at each time point, ensuring a comprehensive assessment of the wound healing process. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | This meticulous procedure provides valuable insights into cell migration dynamics over the specified time intervals. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | For apoptosis assays, the Annexin V-FITC Apoptosis Detection Kit (BD, San Jose, CA, USA) was employed. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Following the manufacturer’s protocol, THP-1 cells were harvested, resuspended in 100 µL of binding buffer, and incubated with 5 µL of annexin V-FITC and 10 µL of propidium iodide (PI) for 15 min in the dark at room temperature. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Subsequently, 400 µL of binding buffer was added, and the stained cells were assessed by flow cytometry after 1 h. This meticulous procedure ensures accurate detection and quantification of apoptotic cells, contributing to a comprehensive analysis of cellular responses. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | HEK293T cells at 30% confluence were carefully seeded into 24-well plates. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | In each well, a combination of the reporter plasmid (100 ng, containing 34 signaling pathways), pRL-CMV (5 ng), and the specified gene-expressing plasmids (Flag-USP5, 500 ng) or an empty vector was transiently transfected into the HEK293T cells. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | This transfection process aimed to introduce the relevant genetic elements into the cells. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Following a 48-hour incubation period, luciferase reporter assays were conducted using a dual luciferase assay kit (E1960, Promega, Beijing). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | This assay provides a sensitive and quantitative measure of gene expression and signaling pathway activities. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The experimental design and execution were meticulous to ensure accurate and reliable results. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Cell lysis was carried out using SDS lysis buffer (62.5 mM Tris-HCl, pH 6.8, 2% SDS, and 10% glycerol) at 95 °C for 10 min. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The total protein was subsequently separated through 10% SDS-PAGE and transferred onto PVDF membranes (IPVH00010; Millipore, Billerica, MA, USA). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Following transfer, the membranes were blocked with 5% skim milk for 1 h to prevent nonspecific binding. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Primary antibodies were then applied to the membranes and allowed to incubate overnight at 4 °C, facilitating specific binding to target proteins. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Subsequently, the membranes were thoroughly washed in TBST before being incubated with HRP-labelled secondary antibodies at room temperature for 1 h, enabling the detection of primary antibody-bound proteins. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Chemiluminescence of the protein bands was evaluated using the Tanon 5500 chemiluminescence image analysis system (Tanon, Shanghai, China). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | GAPDH was utilized as the internal control, ensuring normalization of protein expression levels. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | This method ensures a robust and precise analysis of protein content and expression in the experimental samples. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | To assess the anti-tumor efficacy of circRNA on GISTs cells, we employed a subcutaneous xenograft model in nude mice. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | GIST-T1 and GIST-882 cells, labeled with fluorescence, were subcutaneously injected, with each experimental group comprising a minimum of 5 mice. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The determination of animal numbers per group was meticulously performed through power analysis to ensure statistical robustness. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Randomization of animals into experimental groups was carried out for each study, minimizing potential bias. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | It’s important to note that no blinding of experiment groups was applied in this study. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Tumor volume was diligently monitored on a weekly basis using the formula V = 1/2×Width×Length. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The growth curve, derived from length and time, provided a dynamic representation of tumor development. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Following a 5-week period, fluorescence imaging was conducted to visualize the subcutaneous xenografts in mice. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Subsequently, the mice were euthanized by cervical dislocation in accordance with ARRIVE guidelines to ensure humane and ethical treatment. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | And the subcutaneous xenografts were excised and measured for size and weight. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Histological changes, Ki-67 staining, and TUNEL assay were performed on the subcutaneous tumor tissues to comprehensively examine the impact of circRNA on GISTs cells. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | This multifaceted analysis aimed to provide a thorough understanding of the therapeutic effects at both macroscopic and microscopic levels. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The data are expressed as the mean ± standard deviation unless otherwise specified. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Statistical analyses were performed using SPSS 17.0 software (SPSS Inc., Chicago, IL, USA), for more information, please visit [SPSS official website] (https://www.ibm.com/products/spss-statistics). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | And GraphPad Prism 7 (GraphPad Software, Inc., La Jolla, CA, USA). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Unpaired two-tailed Student’s t-test was applied to assess differences in cell viability, cell migration, invasion ability, cell apoptosis level, xenograft tumor volume, and xenograft tumor weight among various groups. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The paired two-tailed Student’s t-test was employed to compare the expression of circ_SMA4 between GISTs tissues and their paired adjacent tissues. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Spearman’s rho was utilized to evaluate the correlation between different variables. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The chi-square test was conducted to analyze the association between circ_SMA4 expression and the clinicopathological features of the patients. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Kaplan-Meier curves and log-rank tests were utilized to compare the survival of patients with high and low circ_SMA4 expression. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | A significance level of P < 0.05 was considered statistically significant. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Differential expression profiles of circRNAs in GISTs were successfully established. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The cluster heat map in Supplemental Fig. 1A illustrates the top eight upregulated circRNAs (Supplemental Table SII, fold change > 4, P < 0.05) and the top eight downregulated circRNAs (Supplemental Table SIII, fold change > 3, P < 0.05). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Validation through RT-qPCR in 20 GISTs and adjacent tissue pairs confirmed significant upregulation of circ_SMA4 (circRNA_103870) in GISTs tissues (FC = 4.06) compared to adjacent tissues (P < 0.05; Supplemental Fig. 1B). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | To explore the correlation of circ_SMA4 expression with clinical-pathologic features, the 20 GISTs tissue were divided into higher and lower circRNA expression groups based on the median circ_SMA4 expression. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The statistical analysis revealed no significant correlation between circ_SMA4 and the following clinicopathological features: age, gender, tumor size (cm), mitotic figure (HPF), or malignant degrees and type, as well as gene mutation status (P > 0.05). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Chi-square analysis revealed a positive association between circ_SMA4 expression and mitotic figures, malignant degrees (P < 0.05; Supplemental Fig. 1C), while no significant relation was observed with tumor size (P > 0.05; Supplemental Fig. 1C). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Furthermore, the Receiver Operating Characteristic (ROC) curve analysis highlighted the high diagnostic efficiency of circ_SMA4, with an Area Under the Curve (AUC) of 0.9824 (P < 0.01; Figure Supplemental Fig. 1D). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Upon querying the circBase database, has_circRNA_103870 (circBase ID: hsa_circ_0072805) was identified to be located on chromosome 5:69206201–69,206,389. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | It represents one of the most prevalent circRNAs derived from exons and is associated with the gene symbol SMA4; therefore, has_circRNA_103870 is also referred to as circ_SMA4. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | In our study, GISTs cell lines GIST-T1 and GIST-882 were chosen as parental cells, and Human Stomach smooth muscle cells (HGSMC) served as a control. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The results of the RT-qPCR assay revealed a significant upregulation of circ_SMA4 in GIST-T1 and GIST-882 cells compared to HGSMC cells (P < 0.05; Supplemental Fig. 2A). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Also, a slightly lower expression of circ_SMA4 was observed in GIST-T1 cells compared to GIST-882 cells, although this difference was not statistically significant (P > 0.05; Supplemental Fig. 2B). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Additionally, we constructed overexpression and knockdown lentiviral vectors, as well as negative control (NC) vectors for circ_SMA4. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Subsequently, these vectors were stably transfected into GIST-T1 and GIST-882 cells respectively, successfully establishing circ_SMA4 overexpression and knockdown cellular models (P < 0.05; Fig. 1A). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Furthermore, CCK-8 assay, Annexin V-FITC/PI assay, Transwell chamber assay, Cell scratch assay assays were performed to explore the impact of circ_SMA4 on cell proliferation and apoptosis in GISTs. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The CCK-8 assay revealed that circ_SMA4 overexpression significantly promoted GISTs cell proliferation, while circ_SMA4 knockdown dramatically inhibited GISTs cell proliferation (P < 0.05; Fig. 1B). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The Annexin V-FITC/PI assay demonstrated that circ_SMA4 overexpression significantly suppressed GISTs cell apoptosis, whereas circ_SMA4 knockdown significantly promoted GISTs cell apoptosis (P < 0.05; Fig. 1C). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Subsequent assays, including Transwell chamber and cell scratch assays, suggested that circ_SMA4 overexpression markedly promoted GISTs cell invasion and migration, while circ_SMA4 knockdown dramatically inhibited GISTs cell invasion and migration (P < 0.05; Fig. 2A-B). |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Collectively, these findings indicate that circ_SMA4 promotes GISTs proliferation, invasion, and migration while inhibiting apoptosis, thereby playing an oncogenic role in GISTs. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Fig. 1circ_SMA4 Promotes GISTs Cell Proliferation and Inhibits Cell Apoptosis In Vitro. ( |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Successful establishment of circ_SMA4 overexpression and knockdown cellular models. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The transfection efficiency of each group was confirmed through RT-qPCR. ( |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Cell counting kit-8 (CCK8) assay and (C) Annexin V-FITC/PI assay was performed to analyze the effect of circ_SMA4 on GISTs cell proliferation and apoptosis. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | n = 3. * |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | P < 0.05, **P < 0.01. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | RT-qPCR, reverse transcription- quantitative PCR. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | OE: OverExpression. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | shRNA: short hairpin RNA. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | NC: negative control. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | OD, Optical Density. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | circ_SMA4: circ_103870. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | circ_SMA4 Promotes GISTs Cell Proliferation and Inhibits Cell Apoptosis In Vitro. ( |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Successful establishment of circ_SMA4 overexpression and knockdown cellular models. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | The transfection efficiency of each group was confirmed through RT-qPCR. ( |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Cell counting kit-8 (CCK8) assay and (C) Annexin V-FITC/PI assay was performed to analyze the effect of circ_SMA4 on GISTs cell proliferation and apoptosis. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | n = 3. * |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | P < 0.05, **P < 0.01. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | RT-qPCR, reverse transcription- quantitative PCR. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | OE: OverExpression. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | shRNA: short hairpin RNA. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | NC: negative control. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | OD, Optical Density. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | circ_SMA4: circ_103870. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Fig. 2circ_SMA4 Promotes GISTs cell Invasion and Migration In Vitro. ( |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Transwell chamber assay (D) Cell scratch assay was performed to analyze cell invasion and migration ability. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | n = 3. * |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | P < 0.05, **P < 0.01. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | RT-qPCR, reverse transcription- quantitative PCR. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | OE: OverExpression. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | shRNA: short hairpin RNA. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | NC: negative control. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | OD, Optical Density. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | circ_SMA4: circ_103870. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | circ_SMA4 Promotes GISTs cell Invasion and Migration In Vitro. ( |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | Transwell chamber assay (D) Cell scratch assay was performed to analyze cell invasion and migration ability. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | n = 3. * |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | P < 0.05, **P < 0.01. |
PMC11422497 | circ_SMA4 promotes gastrointestinal stromal tumors malignant progression by sponging miR-494-3p/KIT axis and activating JAK/STAT pathway | RT-qPCR, reverse transcription- quantitative PCR. |
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