PMCID string | Title string | Sentences string |
|---|---|---|
PMC12172538 | Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells | In another study, methyltransferase DNMT3B was identified as a factor that stimulates the proliferation of colorectal cancer cells. |
PMC12172538 | Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells | A study has demonstrated that DNMT3A interacts directly with p53, which results in the suppression of p53-mediated transactivation of the p21 gene. |
PMC12172538 | Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells | In this study, it was observed that 1,25(OH)2D3 altered the expression levels of DNMT3A and DNMT3B, but did not cause any change in PTEN expression level in SH-SY5Y cells. |
PMC12172538 | Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells | These results suggest that the methyltransferase effect of DNMT3A and DNMT3B may be effective on other TSGs and different signalling pathways associated with these genes, but not on PTEN. |
PMC12172538 | Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells | Within the limits of this study, the fact that 1,25(OH)2D3 showed antiproliferative, antimigratory activity on neuroblastoma SH-SY5Y cells and decreased the expression levels of genes involved in methylation may be evidence of its anticancer effect. |
PMC12172538 | Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells | In this respect, further and more detailed investigations, both in vitro and in vivo, should be performed to strengthen the evidence of the anticancer effect of 1,25(OH)2D3 in human neuroblastoma. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 (pleckstrin homology-like domain, family A, member 1) is a multifunctional protein involved in pathophysiological conditions such as cancer. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Our previous research revealed changes in the proteome of PHLDA1-silenced human neuroblastoma IMR-32 cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | One of the proteins that was increased in PHLDA1-silenced cells was the efflux pump ABCB1 (ATP binding cassette subfamily B member 1). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In this study, ABCB1 efflux pump upregulation in PHLDA1-silenced IMR-32 cells led to increased resistance of the cells to xenobiotic agents. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Experiments revealed that xenograft tumors grown in immunocompromised mice from PHLDA1-silenced cells presented multiple changes in comparison with tumors grown from control cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Tumors grown from PHLDA1-silenced cells were larger and contained fewer apoptotic cells than control tumors. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The blood vessels within the tumors grown from PHLDA1-silenced cells displayed increased extravasation and the extracellular matrix of the tumors presented increased collagen content in comparison to that of the control tumors. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The results showed that silencing PHLDA1 in vivo promoted human neuroblastoma cell survival, decreased the apoptotic potential of the cells, disrupted angiogenesis in developing tumors, and altered collagen network formation. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 (pleckstrin homology-like domain, family A, member 1) is a protein that plays a role in many cellular functions, and owing to its ambiguous involvement in various types of cancers, this protein is a frequent subject of research. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 is involved in apoptosis and is a confirmed target of the P53 protein. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 participates in the apoptotic process by inhibiting the PI3K/Akt pathway, leading to caspase activation and the induction of cell death in many human cancer cell lines. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 expression may be reduced in certain types of cancer, potentially contributing to uncontrolled cancer cell growth. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 might act as a tumor suppressor and is a negative regulator of aurora A kinase in breast cancer, and downregulation of PHLDA1 mRNA expression is associated with poor prognosis in that cancer. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Moreover, the pleckstrin homology domain is known for binding membrane phospholipids, which suggests that PHLDA1 may be involved in cellular signaling. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 attracted our interest in previous research. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Its expression was upregulated in IMR-32 human neuroblastoma (NB) cells after anti-GD2 14G2a antibody treatment. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Another study revealed that IMR-32 cells in which the PHLDA1 gene was silenced presented increased cellular ATP concentrations and increased mitochondrial potential and were less prone to apoptosis than control cells were. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Moreover, the level of a marker of poor prognosis in neuroblastoma, TRKB (tropomyosin receptor kinase B), was increased in PHLDA1-silenced cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | We showed that silencing of PHLDA1 gene led to a significant increase in the expression of AURKA (encoding Aurora A kinase), increased phosphorylation of p-Aurora A at Thr288 and Akt at Thr308, and lower levels of cleaved PARP and caspase-3 as well as lower activity of apoptosis-executing caspase 3 and − 7. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Thus, the overall results suggested a role of PHLDA1 as a pro-apoptotic protein. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In addition, a significant decrease in the mRNA level of the P21 protein (a product of the CDKN1A gene, which encodes a cell cycle inhibitor), was noted after PHLDA1 silencing. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | By flow cytometry analysis of propidium iodide stained cells, we measured slightly higher percentage of cells in G0/G1 phase for the PHLDA1-silenced clones with down-regulation of PHLDA1 (ca. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | 70%) - as compared to WT and Mock1 cells (ca. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | 65%) and marginally lower number of cells in S and G2/M phases, as compared to WT and Mock1 cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Our most recent study on the IMR-32 human neuroblastoma cell line revealed noticeable changes in the global proteome and phosphoproteome after PHLDA1 silencing, which led to the upregulation of proteins associated with mitochondria. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Among the proteins identified in PHLDA1-silenced cells, ABCB1 was highly upregulated according to mass spectrometry analysis. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 (other names: MDR1, P-GP1) is an efflux pump that exports xenobiotics and other harmful substances from cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | It is strongly involved in the multidrug resistance of cancer cells (MDR). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In human neuroblastoma, multidrug resistance induced by the activity of various ABC transporters often occurs in the tumors of high-risk patients. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 and several other ABC transporters are regulated directly by the MYCN oncogene, which is a major oncogenic driver in neuroblastoma. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In this study, the inverse correlation between PHLDA1 and ABCB1 found in our previous studies via mass spectrometry was confirmed via RT-qPCR and western blotting. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The increased level of ABCB1 after PHLDA1 silencing led to the induction of xenobiotic resistance in IMR-32 and CHP-134 cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Our in vivo experiments revealed that xenograft tumors derived from PHLDA1-silenced cells were larger and more densely packed than control tumor cells, hemorrhagic, and their collagen network was more abundant than that of tumors grown from control cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | These findings point to a role for PHLDA1 in modulating neuroblastoma tumor growth dynamics, ECM remodeling and chemoresistance development. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Our previous study involving global mass spectrometry analysis revealed that the ABCB1 protein, encoding an efflux pump, was found as one of the characteristic significantly enhanced proteome compound in PHLDA1-silenced IMR-32 cells (shP). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | To investigate this finding further, RT‒qPCR and western blot analyses were performed (Fig. 1; Supplementary Fig. S1-S3 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The level of the ABCB1 transcript was more than 15-fold greater in PHLDA1-silenced cells (shP) than in control cells (shC) (Fig. 1a). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The relative PHLDA1 gene expression levels are presented in Fig. 1b. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | As previously reported, PHLDA1-silenced cells are characterized by a significantly decreased level of the PHLDA1 transcript. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The decreased level of PHLDA1 protein is shown in Fig. 1c (as detected with the anti-PHLDA1 antibody Sc-23866 from Santa Cruz Biotechnology). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The nature of the upper band was verified in a separate experiment with an additional anti-PHLDA1 antibody (ab133654 from Abcam, Supplementary Fig. S4 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Therefore, the upper band shown was labeled as an unspecific band. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Moreover, the level of the ABCB1 protein was easily detected in shP cells, although the protein remained undetectable in control shC and WT cells (Fig. 1c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Fig. 1Gene expression analysis of PHLDA1-silenced IMR-32 cells (shP), control cells (shC) and wild-type cells (WT) revealed significant changes in ABCB1 (a) and PHLDA1 (b) transcript levels. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The ABCB1 mRNA content in shC was set as 1. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The PHLDA1 mRNA content in the WT was set as 1. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The data are shown as the means (± SEM) of 3 (a) and 4 (b) independent experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The samples were run in triplicate. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance was calculated with Student’s t test for independent samples (*p < 0.05, exact p = 0.023) (a) and with Kruskal‒Wallis ANOVA followed by Dunn’s test (*p < 0.05, exact p = 0.042) (b). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Western blot analysis of ABCB1 and PHLDA1 in shP, shC and WT IMR-32 cells (c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The HepG2 cell line was used as a positive control for the ABCB1 protein. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | α-Tubulin was used as a reference. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Representative immunoblots of 3 independent experiments are shown (*- unspecific band). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Gene expression analysis of PHLDA1-silenced IMR-32 cells (shP), control cells (shC) and wild-type cells (WT) revealed significant changes in ABCB1 (a) and PHLDA1 (b) transcript levels. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The ABCB1 mRNA content in shC was set as 1. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The PHLDA1 mRNA content in the WT was set as 1. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The data are shown as the means (± SEM) of 3 (a) and 4 (b) independent experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The samples were run in triplicate. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance was calculated with Student’s t test for independent samples (*p < 0.05, exact p = 0.023) (a) and with Kruskal‒Wallis ANOVA followed by Dunn’s test (*p < 0.05, exact p = 0.042) (b). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Western blot analysis of ABCB1 and PHLDA1 in shP, shC and WT IMR-32 cells (c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The HepG2 cell line was used as a positive control for the ABCB1 protein. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | α-Tubulin was used as a reference. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Representative immunoblots of 3 independent experiments are shown (*- unspecific band). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The level of ABCB1 following PHLDA1 silencing was assessed in another human neuroblastoma cell line, CHP-134. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 and ABCB1 levels were measured in PHLDA1-silenced (the S6 and S17 clones), control (the Mock3 clone) and WT cells via western blotting. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The upregulation of ABCB1 following the silencing of PHLDA1 was also confirmed in CHP-134 cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The levels of ABCB1 in the S6 and S17 PHLDA1-silenced clones were greater than those in the Mock3 and WT clones after 48 h of culture in vitro (Supplementary Fig. S5 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The activity of the ABCB1 protein as an efflux pump was examined via two functional assays: a rhodamine 123 accumulation test and a doxorubicin cytotoxicity test. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Rhodamine 123 is a fluorescent dye that is known to be one of the chemicals actively exported by the ABCB1 protein. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | To test ABCB1 protein activity in our model, shP, shC and WT cells were treated with 1 µM rhodamine 123 solution for 4 h, and the fluorescence was measured. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | shC and WT cells displayed high fluorescence intensity because rhodamine 123 accumulated within the cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In contrast, shP cells were nonfluorescent, i.e., they did not accumulate rhodamine 123. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | This means that rhodamine 123 was actively and efficiently pumped out of the shP cells (Fig. 2a; Supplementary Fig. S6 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Fig. 2ABCB1 activity in IMR-32 cells with PHLDA1 silencing. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 activity was verified via a rhodamine 123 accumulation assay (a) and visualized via fluorescence microscopy. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Images of PHLDA1-silenced (shP), control (shC), and WT IMR-32 cells are shown in fluorescence mode, and visible light is presented in the upper and lower panels, respectively. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars – 100 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Fluorescence microphotographs were taken during all three experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Brightfield microphotographs were taken during one experiment (Supplementary Fig. S6 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 functional test after doxorubicin treatment (b). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1-silenced (shP) and control (shC) IMR-32 cells were treated with 30 nM doxorubicin (dox) or water (Ø) for 48 h, protein lysates were subsequently obtained, and western blot analysis was performed in three independent experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Representative immunoblots are shown (*- unspecific band). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | An ATP luminescence test was performed to confirm the cytotoxic effect of doxorubicin (c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The number of control cells treated with water was set to 1. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The samples were run in triplicate. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The data are shown as the means (± SEM) of three independent experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance was assessed by one-way ANOVA, followed by post hoc Tukey’s test (*p < 0.05, exact p = 0.022 for shP water vs. shC water; ***p < 0.001, exact p = 0.00043 for shC water vs. shC doxorubicin; p = 0.000024 for shP doxorubicin vs. shC doxorubicin). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 activity in IMR-32 cells with PHLDA1 silencing. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 activity was verified via a rhodamine 123 accumulation assay (a) and visualized via fluorescence microscopy. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Images of PHLDA1-silenced (shP), control (shC), and WT IMR-32 cells are shown in fluorescence mode, and visible light is presented in the upper and lower panels, respectively. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars – 100 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Fluorescence microphotographs were taken during all three experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Brightfield microphotographs were taken during one experiment (Supplementary Fig. S6 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 functional test after doxorubicin treatment (b). |
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