PMCID string | Title string | Sentences string |
|---|---|---|
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1-silenced (shP) and control (shC) IMR-32 cells were treated with 30 nM doxorubicin (dox) or water (Ø) for 48 h, protein lysates were subsequently obtained, and western blot analysis was performed in three independent experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Representative immunoblots are shown (*- unspecific band). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | An ATP luminescence test was performed to confirm the cytotoxic effect of doxorubicin (c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The number of control cells treated with water was set to 1. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The samples were run in triplicate. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The data are shown as the means (± SEM) of three independent experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance was assessed by one-way ANOVA, followed by post hoc Tukey’s test (*p < 0.05, exact p = 0.022 for shP water vs. shC water; ***p < 0.001, exact p = 0.00043 for shC water vs. shC doxorubicin; p = 0.000024 for shP doxorubicin vs. shC doxorubicin). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Another functional test, an indirect test of ABCB1 protein activity, involved incubating shP and shC cells with doxorubicin (Fig. 2b, c; Supplementary Fig. S7-S10 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The P21 protein is strongly elevated in many types of cells exposed to doxorubicin, including human IMR-32 neuroblastoma cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Therefore, in this test, the protein served as a marker for the cellular response to this chemotherapeutic agent. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1-silenced cells (shP) and control cells (shC) were incubated with complete medium containing 30 nM doxorubicin (or water as the drug diluent) for 48 h, after which the levels of P21, ABCB1 and PHLDA1 were determined (Fig. 2b). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Doxorubicin induced high P21 expression solely in shC cells (Fig. 2b). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Additionally, a significant reduction in ATP levels, by more than 50%, was detected in the doxorubicin-treated shC cells, indicating a strong cytotoxic effect of the chemotherapeutic agent in the control IMR-32 cells (Fig. 2c; Supplementary Fig. S10 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In contrast, the relative ATP levels in shP cells after doxorubicin treatment remained unchanged, indicating that PHLDA1-silenced cells gained resistance to doxorubicin. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | These findings indicate that the ABCB1 efflux pump operates efficiently in PHLDA1-silenced cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Furthermore, we aimed to investigate the response of CHP-134 cells in which PHLDA1 was silenced to doxorubicin to examine whether a similar regulatory effect could be observed in yet another neuroblastoma cell line. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | To assess the cytotoxicity of doxorubicin following PHLDA1 silencing in CHP134, the intracellular ATP content was again measured. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The decreased cytotoxicity of doxorubicin was observed following PHLDA1 silencing in the S6 and S17 clones of CHP-134 neuroblastoma cells compared with that in the control cells, and a statistically significant decrease in ATP levels was detected only at the highest concentration tested (150 nM) (Supplementary Fig. S11 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Finally, IMR-32 cells overexpressing PHLDA1, after stable transfection with a plasmid vector, presented lower ABCB1 levels, which correlated with their increased susceptibility to 30 nM doxorubicin (Supplementary Fig. S12, S13 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Therefore, we can conclude that PHLDA1 affected levels of the ABCB1 transporter in IMR-32 and CHP-134 neuroblastoma cells, which impacted on their sensitivity to doxorubicin. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Athymic nude mice were injected subcutaneously (s.c.) |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | with shP or shC human neuroblastoma IMR-32 cells to determine the impact of PHLDA1 silencing on xenograft tumor growth and morphology. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The silencing of PHLDA1 was monitored before injecting the cells into the mice (Supplementary Fig. S14, S15 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Tumors derived from shP cells showed many differences compared to those from shC cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Macroscopically, tumors derived from shC cells presented light pink coloration, whereas tumors derived from shP cells presented intensely red coloration, indicating a greater blood supply and extravasation (Fig. 3a; Supplementary Fig. S16 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Indeed, numerous extravasations were observed in tumors derived from shP cells, which were absent in tumors derived from shC cells (Supplementary Fig. S17 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Another observed difference was that the cells in the shP tumors were packed thicker than those in the control tumors were (Figs. 3c and 4; Supplementary Fig. S17, S18 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Neuroblastoma tumors with PHLDA1 silencing tended to have greater masses than tumors derived from control cells did (Fig. 3b; Supplementary Table S1a, b online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | This change was clearly visible, although it was statistically significant only for the means of one of the experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Microscopic observations revealed that neuroblastoma tumors derived from implanted shP and shC cells display the typical packed, small round cell tumor architecture, which is characteristic of NB tumors (Fig. 3c; Supplementary Fig. S17 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | They exhibited a nonhomogeneous structure. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | These studies demonstrated that PHLDA1 silencing significantly affected the morphology of neuroblastoma tumors. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The cells within the shP tumors also had more morphologically altered nuclei, indicating frequent mitotic disruptions. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Apoptotic cells, identified on the basis of morphological features and the expression of cleaved PARP-1 and cleaved caspase-3, were statistically significantly more common in shC tumors than in shP tumors (Fig. 3c, d; Supplementary Fig. S19, S20 online; Supplementary Table S2a-f online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | This finding was consistent with our in vitro observations, where more dead cells were present in the microscopic field of view in the shC group during trypan blue staining for cell counting (for passages and experiments). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Fig. 3Impact of PHLDA1 gene silencing on NB tumors grown in vivo in immunocompromised mice. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Photographic images of xenograft neuroblastoma tumors grown from PHLDA1-silenced (shP) and control cells (shC) in the second experiment (a). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Photographs of tumors from two complete experiments are presented in Supplementary Fig. S16 online. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Comparison of the weights of extracted shC and shP tumors in the first and second experiments (b). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The data are presented as the means of 8 tumors per group (± SEM). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The exact tumor weights are shown in the Supplementary Table S1a, b online. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance between the groups was measured with two-tailed Student’s t tests for independent samples. *** |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | p < 0.001, exact p = 0.00016; ns – not statistically significant. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Microscopy images of xenograft neuroblastoma tumors grown from shP and shC cells in immunocompromised mice. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The tissue slices were stained with Meyer’s hematoxylin and eosin (left column), cleaved PARP-1 (middle column), and cleaved caspase-3 (right column) (c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Enlarged fragments of the images containing apoptotic cells identified in HE-stained sections on the basis of morphological features, cleaved PARP-1- and cleaved caspase-3-positive cells are indicated with white rectangles and are shown as inserts. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars, 100 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Mean (± SEM) number of apoptotic cells in the shC and shP groups detected on the basis of morphological features via HE staining, cleaved PARP-1, and cleaved caspase-3 immunostaining, respectively (see Supplementary Table S2a-f online for the counting results from a given tumor sample). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance was assessed via the two-tailed unpaired Student’s t test for independent samples, *p < 0.05, exact p = 0.043 for apoptotic cells; ***p < 0.001, exact p = 9.05 × 10 for cleaved PARP-1-positive cells; ***p < 0.001, exact p = 1.7 × 10 for cleaved caspase-3-positive cells (d). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Impact of PHLDA1 gene silencing on NB tumors grown in vivo in immunocompromised mice. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Photographic images of xenograft neuroblastoma tumors grown from PHLDA1-silenced (shP) and control cells (shC) in the second experiment (a). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Photographs of tumors from two complete experiments are presented in Supplementary Fig. S16 online. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Comparison of the weights of extracted shC and shP tumors in the first and second experiments (b). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The data are presented as the means of 8 tumors per group (± SEM). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The exact tumor weights are shown in the Supplementary Table S1a, b online. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance between the groups was measured with two-tailed Student’s t tests for independent samples. *** |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | p < 0.001, exact p = 0.00016; ns – not statistically significant. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Microscopy images of xenograft neuroblastoma tumors grown from shP and shC cells in immunocompromised mice. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The tissue slices were stained with Meyer’s hematoxylin and eosin (left column), cleaved PARP-1 (middle column), and cleaved caspase-3 (right column) (c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Enlarged fragments of the images containing apoptotic cells identified in HE-stained sections on the basis of morphological features, cleaved PARP-1- and cleaved caspase-3-positive cells are indicated with white rectangles and are shown as inserts. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars, 100 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Mean (± SEM) number of apoptotic cells in the shC and shP groups detected on the basis of morphological features via HE staining, cleaved PARP-1, and cleaved caspase-3 immunostaining, respectively (see Supplementary Table S2a-f online for the counting results from a given tumor sample). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance was assessed via the two-tailed unpaired Student’s t test for independent samples, *p < 0.05, exact p = 0.043 for apoptotic cells; ***p < 0.001, exact p = 9.05 × 10 for cleaved PARP-1-positive cells; ***p < 0.001, exact p = 1.7 × 10 for cleaved caspase-3-positive cells (d). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The xenograft shP tumors were stiff, possibly because of hemorrhages within them and the presence of extracellular matrix (ECM) abnormalities. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In that context, the collagen network was examined in shP and shC cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Masson-Goldner and van Gieson trichrome staining revealed that tumors derived from shP cells contained more collagen fibers and that the fibers were denser than the fibers of tumors derived from shC cells were (Fig. 4; Supplementary Fig. S18 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Fig. 4PHLDA1 silencing augments the collagen net density within tumor tissue. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Microscopy images of xenograft neuroblastoma tumors grown from PHLDA1-silenced (shP) and control cells (shC) in immunocompromised mice. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The tissue slices were stained with Masson-Goldner trichrome or van Gieson trichrome. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars – 100 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The arrows indicate collagen fibers stained with Masson-Goldner trichrome or van Gieson trichrome. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1 silencing augments the collagen net density within tumor tissue. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Microscopy images of xenograft neuroblastoma tumors grown from PHLDA1-silenced (shP) and control cells (shC) in immunocompromised mice. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The tissue slices were stained with Masson-Goldner trichrome or van Gieson trichrome. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars – 100 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The arrows indicate collagen fibers stained with Masson-Goldner trichrome or van Gieson trichrome. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | IMR-32 shP cells used to generate tumors were checked for the occurrence of stable PHLDA1 silencing via western blot analysis (Supplementary Fig. S14, S15 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Tumors derived from shP cells presented a lower intensity of immunohistochemical (IHC) staining with anti-PHLDA1 antibodies (Fig. 5a, b; Supplementary Fig. S21 online; Supplementary Table S3 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | After injection and development into tumors in mice, shP cells presented some level of PHLDA1, but the silencing still persisted. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In shP slices, the mean number of positively stained cells was lower (13 cells per field view) than that in shC slices (24.8 cells per field view; see Fig. 5a, b; Supplementary Table S3 online); however, statistical significance was not reached. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The PHLDA1 level in shP tumors was stably downregulated, compared to tumors derived from shC cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In shC cells, the staining intensity was greater. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | In both shP and shC tumors, PHLDA1 localization was cytoplasmic, however in shC tumors localization of the protein was observed as more intense staining foci. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Fig. 5Expression and localization of the PHLDA1 and ABCB1 proteins in tumors grown in vivo from PHLDA1-silenced and control human IMR-32 cells (a). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Representative microphotographs visualizing immunostaining of shC and shP tumors are shown. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Nuclei were counterstained with Meyer’s hematoxylin. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | A positive control for anti-PHLDA1 antibodies was presented in the mouse kidney. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | A positive control for anti-ABCB1 antibodies was presented on the mouse kidney and mouse liver. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars – 50 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Arrows indicate PHLDA1-positive or ABCB1-positive cells, respectively. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The percentages of positively stained cells are shown in the graphs below (as means ± SEM, see the Supplementary Table S3 online for the counting results from a given tumor sample). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical significance was assessed via the two-tailed unpaired Student’s t test. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | No statistically significant differences in PHLDA1-positive or ABCB1-positive cell numbers were found between shC and shP tumors (b, c). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Expression and localization of the PHLDA1 and ABCB1 proteins in tumors grown in vivo from PHLDA1-silenced and control human IMR-32 cells (a). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Representative microphotographs visualizing immunostaining of shC and shP tumors are shown. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Nuclei were counterstained with Meyer’s hematoxylin. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | A positive control for anti-PHLDA1 antibodies was presented in the mouse kidney. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | A positive control for anti-ABCB1 antibodies was presented on the mouse kidney and mouse liver. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Scale bars – 50 μm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Arrows indicate PHLDA1-positive or ABCB1-positive cells, respectively. |
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