PMCID string | Title string | Sentences string |
|---|---|---|
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Before the experiments, the efficiency of the reaction for each pair of primers was checked. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The cDNA was serially diluted 5-5000, qPCR was performed, and the efficiency of the reaction was calculated in MS Excel via the equation E = 10. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | E = 2 specifies 100% efficiency. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Only starters for which the calculated reaction efficiency was greater than 95% were used in the experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Doxorubicin is a chemotherapeutic agent that is actively removed from cells by the ABCB1 efflux pump. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The cells were treated with a 30 nM solution of doxorubicin (sc-200923, Santa Cruz) (dose selected on the basis of a previous publication or water (solvent) and then seeded at a density of 1 × 10 cells in 5 ml of medium. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The mixture was incubated for 48 h at 37 °C, after which ATP and P21 protein levels were measured as described above. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The experiments were performed three times. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The ABCB1 efflux pump actively removes foreign substances from the inside of the cells. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | One such substance is rhodamine 123, which possesses fluorescent properties. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The cells were seeded at a density of 1 × 10 cells in 5 ml of medium and incubated overnight. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Next, the cells were treated for 4 h with a 1 µM solution of rhodamine 123 (83702, Sigma‒Aldrich), washed with PBS, and observed via a fluorescence microscope (Nikon Eclipse Ti-S, Japan). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The excitation and emission wavelengths were 485 and 539 nm, respectively. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The experiments were performed three times. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1-silenced (shP) and control (shC) cells were grown in 150 cm culture flasks. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The cells were harvested via TrypLeTM reagent (12604021, Gibco) incubation for 1–2 min at 37 °C. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Afterwards, the enzyme was inactivated with 10% FBS in EMEM (with 1% NEAA, 1 mM sodium pyruvate) without the antibiotics gentamycin and puromycin, and the cells were subsequently centrifuged (200 × g, 5 min). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The pellets were resuspended in PBS. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The pools of all resuspended cells were mechanically dispersed to dissociate clumps (as described in) and diluted in PBS for cell counting in a Bürker counting chamber. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The excess cells were then rewashed with PBS (200 × g, 5 min). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | After the removal of the PBS, fresh PBS was added, and the cells were recounted to a specific number for an experiment (with the appropriate excess). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Confirmation of PHLDA1 levels in modified cells prepared for injection into mice was performed via western blotting. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ShP and shC IMR-32 cells (1 × 10 per well of a 6-well plate) were seeded and cultured for 48 h. Afterwards, the cells were harvested and lysed in RIPA buffer supplemented with protease and phosphatase inhibitors. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | After PHLDA1 detection, the membrane was stripped, and then α-tubulin was detected (Supplementary Fig. S14, S15 online). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | To generate xenograft tumors, athymic nude mice (Rj: ATHYM-Foxn1nu/nu, Janvier Lab, France) were used on the basis of procedures approved by the 2nd Local Animal Ethical Committee in Kraków, Poland (Approval no. 153/2022). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | All the experiments were performed in accordance with the relevant guidelines and regulations. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The authors complied with the ARRIVE guidelines. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The mice were housed under pathogen-free conditions in a temperature-controlled (approximately 22 °C) animal facility with a 14/10 hour light/dark cycle (in ventilated cages, with 55 ± 10% humidity) and were fed ad libitum. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The mice were allocated to cages (4 animals per cage) by the animal care staff, but no other blinding/randomization was applied by the researchers. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The animals were housed in the same rack and at the same height for a given experiment. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | No criteria for the exclusion or inclusion of animals were set a priori, and no animals were excluded during the described experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Each of the 2 separate experiments described here involved 16 mice in 2 groups, which were named after the treatment received. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Each experiment included a control group. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The mice (8 females per group, approximately 6 weeks old) were subcutaneously (s.c.) |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | injected without anesthesia in the right flank with 4 × 10 shP or shC IMR-32 cells in Matrigel Matrix HC (354262, Corning; the Matrigel was diluted 1:1 with cells in PBS, and the injection volume was 200 µl). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The s.c. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | tumors were monitored at the inoculation site via caliper measurements to ensure that the tumors did not exceed a volume of 1500 mm. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The weight was monitored to avoid weight loss of more than 20%. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | At the endpoint, the animals were euthanized with carbon dioxide (CO2), and the tumors were collected, weighed, and processed immediately. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The collected tumor samples were incubated for 24 h in 10% formalin (114321730, Chempur), dehydrated in increasing concentrations of ethanol and xylene, and then embedded in paraffin. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The paraffin blocks were stored at 4 °C. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The paraffin blocks were briefly frozen at -20 °C before being cut on a microtome. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | To examine the histology of the tumors, formalin-fixed, paraffin-embedded (FFPE) 4 μm tumor slices were stained with hematoxylin and eosin. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | To examine the collagen net within the tumors, FFPE slices were stained with a Masson trichrome kit (3459.1, Carl Roth GmbH + Co. KG) and a van Gieson Trichrome kit (8275.1, Carl Roth GmbH + Co. KG) according to the manufacturer’s protocol. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Both methods stain connective tissue, but Masson–Goldner staining allows for the visualization and differentiation of muscles and connective tissue (connective tissue is green, muscles are brick red), whereas van Gieson staining differentiates collagen and elastin fibers (elastic fibers are black, and collagen fibers are red). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1, ABCB1, cleaved PARP-1 and cleaved caspase-3 immunohistochemical reactions were performed via standard formalin-fixed, paraffin-embedded (FFPE) 4 μm slices of neuroblastoma tumors. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | ABCB1 antigens were retrieved using low-pH buffer (H-3300, Antigen Unmasking Solution, Citrate-based, pH 6.0; Vector Laboratories). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Endogenous peroxidase activity was blocked with 3% H2O2 solution. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Membrane permeabilization was achieved with 0.1% Triton X-100 reagent. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The tissue sections were incubated overnight with a 1:100 primary antibody mixture (see Table 1) at 4 °C. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The next day, the sections were incubated for 30 min with secondary anti-rabbit antibodies (MP-7451, Vector Laboratories). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | As positive controls, mouse liver and kidney sections were used. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | PHLDA1, cleaved PARP-1 and cleaved caspase-3 antigens were retrieved using high-pH buffer (H-3301, Antigen Unmasking Solution, Tris-based, pH 9.0; Vector Laboratories). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Endogenous peroxidase activity was blocked with 3% H2O2 solution. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The samples were incubated overnight with a 1:40 (PHLDA1), 1:75 (cleaved PARP-1) or 1:50 (cleaved caspase-3) primary antibody mixture (see Table 1). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | For PHLDA1 and cleaved PARP-1, the sections were incubated overnight at 4 °C, and for cleaved caspase-3, they were incubated for 3 h at room temperature. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Then, the sections were incubated for 30 min with secondary anti-rabbit antibodies (MP-7451, Vector Laboratories). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | For PHLDA1, positive controls for immunostaining of mouse kidney sections were used. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The neuroblastoma with the greatest number of morphologically detected apoptotic cells served as a positive control for cleaved PARP-1 and cleaved caspase-3. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The ImmPACT Nova Red (SK-4805, Vector Laboratories) reagent was used to visualize the immunostaining reaction. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Nuclei were counterstained with Mayer’s hematoxylin. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | For PHLDA1 and ABCB1 staining the area of the tumors was examined in five random fields of vision for each group and, the both staining intensity, evaluated as 0-negative, 1-weak, 2-moderate, 3-strong. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Then the percentage of IHC-positive cells was assessed and used for the staining index calculation according to the formula: [% x staining intensity]. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Apoptosis in hematoxylin and eosin-stained samples was identified based on morphology. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The number of cells with pyknotic nuclei and apoptotic bodies was counted under a bright-field microscope (Olympus Optical Co., Tokyo, Japan) with a 40x objective. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Five adjacent fields of view present on each slide were examined. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Apoptotic cells were also detected with an anti-cleaved PARP-1 antibody and an anti-cleaved caspase-3 antibody, and the number of cells with a positive IHC signal was counted with a 40x objective. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The results of the counting of apoptotic cells are shown in the Supplementary Table S2a-f online. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Bioinformatic screening was performed via the R2 platform (http://r2.amc.nl, http://r2platform.com). |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The Versteeg 88 dataset was used for the analyses. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Each of the in vitro experiments was conducted at least three times. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | The results are presented as the means (± SEM) of independent experiments. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Statistical analyses of experiments involving two groups were performed via two-tailed Student’s t test for independent samples. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Other experiments were statistically analyzed via the Kruskal‒Wallis ANOVA test, followed by Dunn’s post hoc analysis or one-way ANOVA followed by the post hoc Tukey’s test. |
PMC12738729 | PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors | Calculations were performed via Excel software (Microsoft), R (R version 3.2.1 Patched), and Graph Pad Prism 6. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | In our previous study on Admission Delirium Tremens (ADT), a severe form of alcohol withdrawal marked by tremors and confusion, we found a significant increase in the neuronal biomarker neurofilament light chain (NfL) . |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | Although haloperidol, used for DT by antagonizing dopamine D2 and serotonin 5-HT2A receptors ., |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | was once a common treatment, it did not impact the duration of DT and is no longer the primary therapy. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | Genetic association analyses revealed a correlation between the serotonin 5-HT2A receptor gene (HTR2A) and NfL. This study aims to investigate whether the expression level of HTR2A correlates with the release of NfL in cell culture media. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | A total of 221 alcohol-dependent patients were enrolled for genotyping verification. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | Plasma and culture media NfL levels were measured using the Simple Plex™ NfL Assay on the Ella™ instrument, following the manufacturer's instructions. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | HTR2A SNPs were selected from the imputed dataset generated by the Michigan Imputation Server, and the polymorphism rs9567746 was validated using TaqMan Real-Time PCR assays. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | Its association with NfL levels was then evaluated through linear regression analysis. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | The SK-N-SH human neuroblastoma cell line was cultured, and HTR2A expression was knocked down using HTR2A siRNA targeting exon 4 (1466–1486, NM_000621.5) in the presence or absence of TNF-α or haloperidol. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | HTR2A mRNA expression levels were quantified by real-time PCR. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | Data visualization was conducted using GraphPad Prism 5 software, with statistical significance set at P < 0.05. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | ADT patients exhibited significantly higher plasma NfL levels compared to non-DT patients and controls. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | Notably, DT patients’ HTR2A genetic polymorphisms located at intron 3 were significantly associated with NfL levels (P = 0.001). |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | Carriers of the minor allele at rs9567746 showed significantly elevated NfL levels than the major genotype carriers. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | In vitro, selective knockdown of HTR2A significantly increased NfL release in SK-N-SH cell culture media, particularly when co-incubated with TNF-α (100 ng/mL). |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | These findings suggest a mechanistic link between HTR2A expression and NfL release. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | In rs9567746 polymorphism of HTR2A, the ADT patient’s minor allele type carrier, present in approximately 20% of ADT patients, showed significant increase of plasma NfL levels than major genotype carriers. |
PMC12359789 | 433. HTR2A EXPRESSION LEVELS ARE CORRELATED WITH THE RELEASE OF NEUROFILAMENT LIGHT CHAIN | This minor genotype carriers could be having lower HTR2A expression, this could be demonstrated by HTR2A knock-down and increase the NfL release through pathways with or without TNF-α, highlighting the low expression of HTR2A could be a pathway toward more severe brain neuronal injury in ADT. |
PMC12732662 | Pharmacological Inhibition of JNK Signalling Exerts Anti-Neoplastic Effects on SH-SY5Y Human Neuroblastoma Cells | Neuroblastoma (NB) is the most prevalent paediatric extracranial solid tumour, which remains a major therapeutic challenge, especially in cases of recurrent and disseminated disease. |
PMC12732662 | Pharmacological Inhibition of JNK Signalling Exerts Anti-Neoplastic Effects on SH-SY5Y Human Neuroblastoma Cells | c-Jun N-terminal kinases (JNKs) are increasingly evidenced to play a key role in NB tumourigenesis and progression through apoptosis regulation, making selective JNK inhibitors promising candidates for use in targeted anticancer drugs in NB. |
PMC12732662 | Pharmacological Inhibition of JNK Signalling Exerts Anti-Neoplastic Effects on SH-SY5Y Human Neuroblastoma Cells | Our study comprehensively investigated the acute antineoplastic potential of the selective JNK inhibitor AS601245 (JNK inhibitor V) on the human MYCN-non-amplified neuroblastoma cell line, SH-SY5Y, with particular focus on its effects on NB cell viability, proliferation, migration, apoptosis, gene and protein expression, and mitochondrial metabolism. |
PMC12732662 | Pharmacological Inhibition of JNK Signalling Exerts Anti-Neoplastic Effects on SH-SY5Y Human Neuroblastoma Cells | JNK V selectively impaired NB cell survival and function, without exerting cytotoxicity toward normal human Schwann cells (HSC) and fibroblasts (BJ). |
PMC12732662 | Pharmacological Inhibition of JNK Signalling Exerts Anti-Neoplastic Effects on SH-SY5Y Human Neuroblastoma Cells | Our findings highlighted a dose-dependent inhibition of proliferation (XTT assay), colony formation (clonogenic assay), and migration (wound healing assay), accompanied by increased caspase-3 activity (caspase-3 assay), pro-apoptotic genes (qRT-PCR) and protein (Western blotting) expression, and significant disruption of both oxidative phosphorylation and glycolysis (Agilent Seahorse XF Assay). |
PMC12732662 | Pharmacological Inhibition of JNK Signalling Exerts Anti-Neoplastic Effects on SH-SY5Y Human Neuroblastoma Cells | These results provide new insights into the therapeutic potential of JNK inhibition as a targeted strategy for NB.Neuroblastoma (NB) is the most frequently occurring paediatric extracranial solid tumour, constituting up to 15% of all cancer deaths in children . |
PMC12732662 | Pharmacological Inhibition of JNK Signalling Exerts Anti-Neoplastic Effects on SH-SY5Y Human Neuroblastoma Cells | NB is an embryonal neuroendocrine neoplasm of the sympathetic nervous system, originating from neural crest progenitor cells, which undergo maladaptive differentiation because of genomic and epigenetic defects . |
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