id stringlengths 4 8 | title stringlengths 1 642 | abstract stringlengths 50 17.9k |
|---|---|---|
12584765 | Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties. | It has been found that lipase from Pseudomonas fluorescens (PFL) is able to aggregate into bimolecular structures (MW around 66 kD) even at moderate enzyme concentrations. At very low enzyme concentrations and in the presence of detergents, the same enzyme displayed a unimolecular structure with a molecular weight of 3... |
14528696 | [Study on stability of beta-cyclodextrin to chlorogenic acid]. | To investigate the stabilizing effect of chlorogenic acid against beta-cyclodextrin, beta-cyclodextrin inclusion compound of chlorogenic acid was prepared by liquid phase method, and affirmed by UV and different thermal analysis. Stability in solution was contrasted between chlorogenic acid and its inclusion complex wi... |
12967266 | Membrane peptides and their role in protobiological evolution. | How simple membrane peptides performed such essential protocellular functions as transport of ions and organic matter across membranes separating the interior of the cell from the environment, capture and utilization of energy, and transduction of environmental signals, is a key question in protobiological evolution. O... |
12961852 | [Studies on the formation of cyclodextrin nanotube by fluorescence and anisotropy measurements]. | Steady-state fluorescence and anisotropy measurements have been used to investigate the interaction of 2,2'-biquinoline (BQ) and 1,1'-(methylenedi-1,4-phenylene)bismaleimide (MDP-BMI) with alpha-, beta-, and gamma-cyclodextrins (CDs). It was found that the reaction patterns between fluorescence molecules and CDs are re... |
12929675 | Why a hexabromodiquinoline host preferentially includes small aromatic hydrocarbon guests. | The preparation and properties of the new hexabromodiquinoline derivative 4 are described. This lattice inclusion host shows a strong preference for trapping small aromatic hydrocarbons. The X-ray crystal structures of the benzene, toluene, o-xylene, and p-xylene compounds are reported, and are analysed from a crystal ... |
12929657 | The utility of cyclodextrins in lipase-catalyzed transesterification in organic solvents: enhanced reaction rate and enantioselectivity. | The use of enzymes as valuable catalysts in organic solvents has been well documented. However, some of their features limit their application in organic synthesis, especially the frequently lower enzyme activity under nonaqueous conditions, which constitutes a major drawback in the application of enzymes in organic so... |
12927053 | Cytokine gene expression in monocytes of patients undergoing cardiopulmonary bypass surgery evaluated by real-time PCR. | Cardiopulmonary bypass (CPB) surgery induces systemic release of proinflammatory cytokines causing unspecific inflammatory reactions. This study deals with the development of a sensitive technique for detecting changes at the mRNA level in monocytes of patients undergoing CPB surgery, by using real-time PCR. Blood samp... |
12926401 | Carboxylic acid clathrate hosts of Diels-Alder adducts of phencyclone and 2-alkenoic acids. Role of bidentate C-H ... O hydrogen bonds between the phenanthrene and carbonyl groups in host-host networks. | Carboxylic acid host compounds (3) having a phenanthrene-condensed bicyclo[2.2.1]hept-2-en-7-one skeleton have been synthesized by the [4 + 2]pi cycloaddition of phencyclone (1a) with 2-alkenoic acids (2) and their inclusion behavior was investigated. The endo [4 + 2]pi cycloadducts (3) enclathrated alcohols and ethers... |
12926398 | The synthesis and studies towards the self-replication of bis(capped porphyrins). | Connecting two facially-protected porphyrins was expected to lead to an equal mixture of laterally-bridged doubly-protected bis-porphyrins; one in which the two porphyrin units were protected on the same face (syn) and one with the two prophyrin units protected on opposite faces (anti). Addition of a co-factor (bidenta... |
12926397 | 1,3-bis(diarylmethylidene)-2-methylidenecyclohexanes in cycloaddition and cyclodimerization reactions. The role of stereoelectronic factors. | Cycloaddition and cyclodimerization reactions of 1,3-dibenzylidene- and 1,3-bis(p-methoxybenzylidene)-2-methylidenecyclohexanes, i.e., the diaryltrienes with fixed S-cis-configurations of exocyclic double bonds are studied. These compounds undergo in situ cyclodimerization of the [4 + 2]-type upon dehydration of the pr... |
12926390 | The synthesis of trianglimines: on the scope and limitations of the [3 + 3] cyclocondensation reaction between (1R,2R)-diaminocyclohexane and aromatic dicarboxaldehydes. | The synthesis of aromatic dicarboxaldehydes, using dilithiation methodology is described along with their reactivity, in the [3 + 3] cyclocondensation reaction, with (1R,2R)-diaminocyclohexane to give trianglimine macrocycles. The scope and limitations of the cyclocondensation reaction are studied and some comments on ... |
12882150 | Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosum. | Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytuber... |
12852447 | Enantioseparation of erythro-mefloquine and its analogues in capillary electrophoresis. | The enantioseparations of the chiral antimalaria drug (R,S)-erythro-alpha-(2-piperidyl)-2,8-bis(trifluoromethyl)4-quinolinemethanol (erythro-mefloquine, erythro-MQ) and its analogues were studied by capillary electrophoresis (CE) using cyclodextrins (CDs) as chiral selectors. The emphasis was put on the enantiomer affi... |
12848291 | Enhancement of G protein-coupled signaling by DHA phospholipids. | The effect of phospholipid acyl chain and cholesterol composition on G protein-coupled signaling was studied in native rod outer segment (ROS) disk and reconstituted membranes by measuring several steps in the visual transduction pathway. The cholesterol content of disk membranes was varied from 4 to 38 mol% cholestero... |
12817648 | NMR evidence of a supramolecular structure of water. | The hypothesis advanced in this paper is based on the formation of dissipative structures, in the form of supramolecular structures of water, by virtue of its hydrogen bonds, structurally similar to a liquid crystal. The supramolecular structure could be a macromolecule typical of a living organism (plant or animal), w... |
12806827 | Hydrolytic properties of per (3,6-anhydro, 2-O-carboxymethyl) alpha cyclodextrin complexes of Ce (III) and Eu (III): application to soman (GD) degradation. | Per (3,6-anhydro-2-O-carboxymethyle) alpha-cyclodextrin ([ACX]) is a polydentate analog of EDTA, a well-known cation chelating reagent. ACX exhibits strong affinities in vitro for uranyl, cobalt and also for lanthanids such as Europium and Cerium. The hydrolytic activities of ACX-Eu and ACX-Ce complex were directly tes... |
12804063 | Intraocular availability of topically applied mycophenolate mofetil in rabbits. | The efficacy of mycophenolate mofetil (MMF), a prodrug of mycophenolic acid (MPA), in high risk keratoplasties and ocular immune-mediated diseases has been shown in recent years. As peroral administration of MMF can cause various side effects, topical application should be considered. This study investigates the intrao... |
12803804 | Capillary electrophoretic behaviors of pharmacologically active xanthones from Securidaca inappendiculata with beta-cyclodextrin as a buffer additive. | The capillary electrophoretic (CE) behaviors of ten xanthones in the presence of beta-cyclodextrin (CD) are investigated, and apparent analyte-selector binding constants between beta-CD and the xanthones in the CE running buffer are calculated to elucidate the migration order. Also, the separation selectivity with beta... |
12671029 | Cyclodextrins differentially mobilize free and esterified cholesterol from primary human foam cell macrophages. | Human monocyte-derived foam cell macrophages (HMFCs) are resistant to cholesterol efflux mediated by physiological acceptors. The role of the plasma membrane in regulating depletion of free cholesterol (FC) and of cholesteryl ester (CE) was investigated using cyclodextrins (CDs). HMFCs were incubated in media containin... |
12787893 | Inclusion complexes of ketosteroids with beta-cyclodextrin. | Inclusion complexes of several steroid derivatives with beta-cyclodextrin (7) were studied in dimethylsulfoxide solution. The investigated molecules were ketosteroids with different functional groups on the skeleton: 3beta-acetoxypregn-5-en-20-one (1), 3beta-acetoxypregna-5,16-dien-20-one (2), 3beta-acetoxyandrost-5-en... |
12785261 | Studies on intra-supramolecular and intermolecular electron-transfer processes between zinc naphthalocyanine and imidazole-appended fullerene. | Spectroscopic, computational, redox, and photochemical behavior of a self-assembled donor-acceptor dyad formed by axial coordination of zinc naphthalocyanine, ZnNc, and fulleropyrrolidine bearing an imidazole coordinating ligand (2-(4'-imidazolylphenyl)-fulleropyrrolidine, C60Im) was investigated in noncoordinating sol... |
12785715 | Toward an understanding of cell growth and the cell division cycle of unicellular photoautotrophic cyanobacteria. | The cell division cycle of Synechococcus sp. strain PCC 6301 in light is characterized by the sequential and orderly appearance of macromolecular synthesis periods. In the dark, macromolecular synthesis and cell division are severely curtailed. When dark-incubated cultures are reexposed to light, a new cell cycle is in... |
12785714 | Molecular adaptations to cold in psychrophilic enzymes. | Psychrophiles or cold-loving organisms successfully colonize cold environments of the Earth's biosphere. To cope with the reduction of chemical reaction rates induced by low temperatures, these organisms synthesize enzymes characterized by a high catalytic activity at low temperatures associated, however, with low ther... |
12782141 | Crystal structure of the collagen alpha1(VIII) NC1 trimer. | Collagen VIII is a major component of Descemet's membrane and is also found in vascular subendothelial matrices. The C-terminal non-collagenous domain (NC1) domain of collagen VIII, which is a member of the C1q-like protein family, forms a stable trimer and is thought to direct the assembly of the collagen triple helix... |
12779224 | Mixed chiral stationary phase containing modified resorcinarene and beta-cyclodextrin selectors bonded to a polysiloxane for enantioselective gas chromatography. | Both a resorcinarene with pendant L-valine diamide groups (used as hydrogen-bonding selector) and a permethylated beta-cyclodextrin (used as inclusion-type selector) were chemically bonded to poly(hydromethyl)dimethylsiloxane in a one-pot reaction via Pt-catalyzed alkene hydrosilylation. This novel mixed chiral station... |
12775156 | Sex pheromone of the vine mealybug, Planococcus ficus in Israel: occurrence of a second component in a mass-reared population. | Two pheromonal components were detected in airborne collections from the vine mealybug Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae) mass-reared on potato sprouts. The compounds were identified as (S)-lavandulyl senecioate (I) and (S)-lavandulyl isovalerate (II) by GC and GC-MS by comparison with synthetic s... |
12769991 | Structural and biochemical analysis of the Leptinotarsa decemlineata (Coleoptera; Chrysomeloidea) crystalline chorionic layer. | The developmental aspects of the Leptinotarsa decemlineata crystalline chorionic layer (CCL) morphogenesis, its composition and its supramolecular structure were studied. The mature Leptinotarsa decemlineata eggshell consists of the vitelline membrane and the CCL, while the follicle cell remnants following their degene... |
12769230 | Evaluation of the antiulcerogenic activity of violacein and its modulation by the inclusion complexation with beta-cyclodextrin. | The effects of beta-cyclodextrin (betaCD) inclusion complexation on the ability of violacein to prevent gastric ulceration in mice were studied. Violacein-betaCD inclusion complexes were prepared in 1:1 and 1:2 molar ratios and analysed by differential scanning calorimetry and powder X-ray diffractometry. Violacein pre... |
14755938 | [Cyclodextrins implications in drugs control and analysis]. | The discovery and development of substances require new forms at a level which was considered satisfactory a few years ago. The research on secondary effects and drugs optimizations to new pharmaceutical forms. So, we can speak about the inclusion complexes. The purpose of the paper was to describe the cyclodextrins pr... |
12752382 | The transient depression of hippocampal CA1 LTP induced by chronic intermittent ethanol exposure is associated with an inhibition of the MAP kinase pathway. | Using electrophysiological and biochemical approaches, we investigated the effects of chronic, intermittent ethanol (CIE) treatment on activation of the mitogen activated protein kinase (MAPK), also known as extracellular signal regulated protein kinase 1 and 2. In hippocampal slices taken from control rats, brief high... |
12668760 | Structure and assembly of an augmented Sm-like archaeal protein 14-mer. | To better understand the roles of Sm proteins in forming the cores of many RNA-processing ribonucleoproteins, we determined the crystal structure of an atypical Sm-like archaeal protein (SmAP3) in which the conserved Sm domain is augmented by a previously uncharacterized, mixed alpha/beta C-terminal domain. The structu... |
12668642 | Quantitation of CD8+ T cell responses to newly identified HLA-A*0201-restricted T cell epitopes conserved among vaccinia and variola (smallpox) viruses. | Immunization with vaccinia virus resulted in long-lasting protection against smallpox and was the approach used to eliminate natural smallpox infections worldwide. Due to the concern about the potential use of smallpox virus as a bioweapon, smallpox vaccination is currently being reintroduced. Severe complications from... |
12734626 | Separation of cocaine stereoisomers by capillary electrophoresis using sulfated cyclodextrins. | A capillary electrophoretic method for the separation of cocaine and its stereoisomers was developed. In this study, the effect of organic modifier was also investigated. The separation was achieved using 1% sulfated cyclodextrin, 10 mmol L(-1) phosphate buffer, 10% methanol at pH 3. The method provides good reproducib... |
12663860 | Induced nucleotide specificity in a GTPase. | In signal-recognition particle (SRP)-dependent protein targeting to the bacterial plasma membrane, two GTPases, Ffh (a subunit of the bacterial SRP) and FtsY (the bacterial SRP receptor), act as GTPase activating proteins for one another. The molecular mechanism of this reciprocal GTPase activation is poorly understood... |
12663652 | Organization of the G protein-coupled receptors rhodopsin and opsin in native membranes. | G protein-coupled receptors (GPCRs), which constitute the largest and structurally best conserved family of signaling molecules, are involved in virtually all physiological processes. Crystal structures are available only for the detergent-solubilized light receptor rhodopsin. In addition, this receptor is the only GPC... |
12657820 | Molecular structures of the inclusion complexes beta-cyclodextrin-1,2-bis(4-aminophenyl)ethane and beta-cyclodextrin-4,4'-diaminobiphenyl; packing of dimeric beta-cyclodextrin inclusion complexes. | The present investigation is part of an ongoing study on the influence of the long end-functonalized guest molecules DBA and BNZ in the crystal packing of beta-cyclodextrin (betaCD) dimeric complexes. The title compounds are 2:2 host:guest complexes showing limited host-guest hydrogen bonding at the primary faces of th... |
12657818 | supramolecular structures of N(4)-substituted 2,4-diamino-6-benzyloxy-5-nitrosopyrimidines. | The molecular and supramolecular structures of eight N(4)-substituted 2,4-diamino-6-benzyloxy-5-nitrosopyrimidines are discussed, along with one analogue containing no nitroso substituent. The nitroso derivatives all exhibit polarized molecular-electronic structures leading to extensive charge-assisted hydrogen bonding... |
12657817 | Phosphonopropionic acid as a building block in supramolecular chemistry: salts with organic diamines. | The structures of seven salts formed by phosphonopropionic acid with organic diamines are reported; in these salts, the hydrogen-bonded substructures formed by the anions can be zero-, one- or two-dimensional, while the overall hydrogen-bonded supramolecular structures are three-dimensional. The 1:1 adduct, compound (1... |
12657643 | Differential mobilization of newly synthesized cholesterol and biosynthetic sterol precursors from cells. | Previous work demonstrates that the biosynthetic precursor of cholesterol, desmosterol, is released from cells and that its efflux to high density lipoprotein or phosphatidylcholine vesicles is greater than that of newly synthesized cholesterol (Johnson, W. J., Fischer, R. T., Phillips, M. C., and Rothblat, G. H. (1995... |
12655062 | Enterobactin: an archetype for microbial iron transport. | Bacteria have aggressive acquisition processes for iron, an essential nutrient. Siderophores are small iron chelators that facilitate cellular iron transport. The siderophore enterobactin is a triscatechol derivative of a cyclic triserine lactone. Studies of the chemistry, regulation, synthesis, recognition, and transp... |
12655056 | The bioinorganic chemistry of iron in oxygenases and supramolecular assemblies. | The bioinorganic chemistry of iron is central to life processes. Organisms must recruit iron from their environment, control iron storage and trafficking within cells, assemble the complex, iron-containing redox cofactors of metalloproteins, and manage a myriad of biochemical transformations by those enzymes. The coord... |
12655053 | Salmochelins, siderophores of Salmonella enterica and uropathogenic Escherichia coli strains, are recognized by the outer membrane receptor IroN. | Members of a family of catecholate siderophores, called salmochelins, were isolated by reversed-phase HPLC from Salmonella enterica serotype Typhimurium and structurally characterized by Fourier transform ion cyclotron resonance-MSMS and GC-MS. The tentative structure of salmochelin 1 contained two 2,3- dihydroxybenzoy... |
12655051 | New clues for platinum antitumor chemistry: kinetically controlled metal binding to DNA. | From the metal ions and metal compounds that are known to bind to DNA, many anticancer Pt(II) and Ru(II)Ru(III) compounds are known to have ligand-exchange kinetics in the same order of magnitude as the division of tumor cells. The present article discusses this process in detail with special attention to cisplatin and... |
12652302 | A defective response to Hedgehog signaling in disorders of cholesterol biosynthesis. | Smith-Lemli-Opitz syndrome (SLOS), desmosterolosis and lathosterolosis are human syndromes caused by defects in the final stages of cholesterol biosynthesis. Many of the developmental malformations in these syndromes occur in tissues and structures whose embryonic patterning depends on signaling by the Hedgehog (Hh) fa... |
12806128 | Amylin in the periphery. | Amylin (islet amyloid polypeptide) is a peptide synthesized principally in the beta-cells of the pancreatic islets together with insulin and has actions as a hormone, growth factor, and modifier of behavior. As a hormone, amylin acts to modify gastric motility, renal resorption, and has metabolic actions. It is postula... |
12806126 | Chemical stabilisation of collagen as a biomimetic. | Collagen is the most abundant protein in animals and because of its high mechanical strength and good resistance to degradation has been utilized in a wide range of products in industry whilst its low antigenicity has resulted in its widespread use in medicine. Collagen products can be purified from fibres, molecules r... |
12659661 | Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance. | <h4>Background</h4>A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20-200 micro-grams total RNA or 0.5-2 micro-grams poly (A) RNA is typically required for monitoring gene expression. In addition, gene expression profiles from large, heterogeneous... |
12719860 | [Antipruritic effects of pimecrolimus and tacrolimus]. | The development of topical calcineurin inhibitors resulted in a significant improvement in the treatment of inflammatory skin diseases such as atopic dermatitis. In addition, an excellent amelioration of pruritus could be observed. Other itchy dermatoses such as chronic irritative hand dermatitis, rosacea, graft-versus... |
12682389 | Diaqua(2,2'-bipyridine)malonatomanganese(II). | In the crystal structure of the title compound, [Mn(C(3)H(2)O(4))(C(10)H(8)N(2))(H(2)O)(2)], the Mn(II) atom demonstrates a distorted octahedral geometry, being coordinated by two N atoms of a 2,2'-bipyridine ligand, two O atoms from the carboxylate groups of the chelating malonate dianion and two O atoms of two cis wa... |
12651957 | Canonical and unconventional pairing schemes between bis(nucleobase) complexes of trans-a2PtII: artificial nucleobase quartets and C-H...N bonds. | If two nucleobases are crosslinked by trans-a(2)Pt(II), self-association via H bonding may take place either through individual bases or jointly through both bases. Due to the blockage of an acceptor site by the metal, the number of feasible pairing patterns can be reduced, and the preferred ones altered. If the metala... |
12651950 | A redox switch in CopC: an intriguing copper trafficking protein that binds copper(I) and copper(II) at different sites. | The protein CopC from Pseudomonas syringae has been found capable of binding copper(I) and copper(II) at two different sites, occupied either one at a time or simultaneously. The protein, consisting of 102 amino acids, is known to bind copper(II) in a position that is now found consistent with a coordination arrangemen... |
12654724 | A divergent INS protein in Caenorhabditis elegans structurally resembles human insulin and activates the human insulin receptor. | Caenorhabditis elegans contains a family of putative insulin-like genes proposed to regulate dauer arrest and senescence. These sequences often lack characteristic sequence features of human insulin essential for its folding, structure, and function. Here, we describe the structure and receptor-binding properties of IN... |
12857855 | The hippocampal laminin matrix is dynamic and critical for neuronal survival. | Laminins are extracellular matrix proteins that participate in neuronal development, survival, and regeneration. During excitotoxin challenge in the mouse hippocampus, neuron interaction with laminin-10 (alpha5,beta1,gamma1) protects against neuronal death. To investigate how laminin is involved in neuronal viability, ... |
12733014 | Comparison of the voltammetric studies at mercury and glassy carbon electrodes for the interaction of lumichrome with DNA and analytical applications. | The determination of the interaction between lumichrome (LC), one of the products of decomposition of the biologically important flavins, and calf thymus double-stranded DNA was performed by using cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV) in connection with a hanging mercury drop elect... |
12688943 | Structural and electronic properties of metal-encapsulated silicon clusters in a large size range. | Structural and electronic properties of metal-doped silicon clusters MSi(n)s (M=W, Zr, Os, Pt, Co, etc.) in a large size range of 8<or=n<or=20 are investigated via ab initio calculations. Different from a recent experimental suggestion that the metal atom is endohedral in MSi(n), we reveal that the formation of endohed... |
12646707 | The CsrR/CsrS two-component system of group A Streptococcus responds to environmental Mg2+. | Group A streptococci control expression of key virulence determinants via the two-component sensorregulator system CsrRCsrS. The membrane-bound sensor CsrS is thought to respond to previously unknown environmental signal(s) by controlling phosphorylation of its cognate regulator component CsrR. Phosphorylation of CsrR ... |
12644710 | Structural effects of quinacrine binding in the open channel of the acetylcholine receptor. | Noncompetitive inhibitors of the nicotinic acetylcholine (ACh) receptors suppress cation flux directly by binding in and blocking the open channel or indirectly by stabilizing closed states of the receptor. The lidocaine derivative QX-314 and the acridine derivative quinacrine act directly as open channel blockers, but... |
12642673 | ARC5, a cytosolic dynamin-like protein from plants, is part of the chloroplast division machinery. | Chloroplast division in plant cells is orchestrated by a complex macromolecular machine with components positioned on both the inner and outer envelope surfaces. The only plastid division proteins identified to date are of endosymbiotic origin and are localized inside the organelle. Employing positional cloning methods... |
12642670 | Speculative synthetic chemistry and the nitrogenase problem. | There exist a limited but growing number of biological metal centers whose properties lie conspicuously outside the realm of known inorganic chemistry. The synthetic analogue approach, broadly directed, offers a powerful exploratory tool that can define intrinsic chemical possibilities for these sites while simultaneou... |
12642669 | A duodenally absorbable CXC chemokine receptor 4 antagonist, KRH-1636, exhibits a potent and selective anti-HIV-1 activity. | A low molecular weight nonpeptide compound, KRH-1636, efficiently blocked replication of various T cell line-tropic (X4) HIV type 1 (HIV-1) in MT-4 cells and peripheral blood mononuclear cells through the inhibition of viral entry and membrane fusion via the CXC chemokine receptor (CXCR)4 coreceptor but not via CC chem... |
12642662 | Dealing with iron: common structural principles in proteins that transport iron and heme. | Iron is essential to life, but poses severe problems because of its toxicity and the insolubility of hydrated ferric ions at neutral pH. In animals, a family of proteins called transferrins are responsible for the sequestration, transport, and distribution of free iron. Comparison of the structure and function of trans... |
12806121 | AFM imaging of lipid domains in model membranes. | Characterization of the two-dimensional organization of biological membranes is one of the most important issues that remains to be achieved in order to understand their structure-function relationships. According to the current view, biological membranes would be organized in in-plane functional microdomains. At least... |
12642593 | Palmitoylation regulates regulators of G-protein signaling (RGS) 16 function. I. Mutation of amino-terminal cysteine residues on RGS16 prevents its targeting to lipid rafts and palmitoylation of an internal cysteine residue. | Regulators of G-protein signaling (RGS) proteins down-regulate signaling by heterotrimeric G-proteins by accelerating GTP hydrolysis on the G alpha subunits. Palmitoylation, the reversible addition of palmitate to cysteine residues, occurs on several RGS proteins and is critical for their activity. For RGS16, mutation ... |
12682728 | Characterization of the human autoimmune response to the major C-terminal epitope of the ribosomal P proteins. | Autoantibodies to the ribosomal phospho (-P) proteins P0, P1, and P2, collectively referred to as Rib-P, are specifically found in 10-40% of patients with systemic lupus erythematosus (SLE). These antibodies are believed to be correlated with lupus nephritis, hepatitis, and central nervous system involvement. The major... |
12654735 | Structure-activity relationships for a series of peptidomimetic antimicrobial prodrugs containing glutamine analogues. | Synthetic glutamine analogues such as N3-(4-methoxyfumaroyl)-l-2,3-diaminopropanoic acid (FMDP) inhibit purified glucosamine-6-phosphate synthase, an intracellular enzyme that is essential for microbial cell wall synthesis, but they are inactive against intact organisms because they cannot enter the cell. However, when... |
12637320 | Membrane cholesterol regulates LFA-1 function and lipid raft heterogeneity. | Many surface receptors and signaling molecules are thought to associate with unique membrane microdomains termed lipid rafts. We examined the involvement of lipid rafts in the activation of leukocyte function-associated antigen-1 (LFA-1). Depletion or sequestration of cholesterol with methyl-beta-cyclodextrin (MCD) or ... |
12634425 | Opening protein pores with chaotropes enhances Fe reduction and chelation of Fe from the ferritin biomineral. | Iron is concentrated in ferritin, a spherical protein with a capacious cavity for ferric nanominerals of <4,500 Fe atoms. Global ferritin structure is very stable, resisting 6 M urea and heat (85 degrees C) at neutral pH. Eight pores, each formed by six helices from 3 of the 24 polypeptide subunits, restrict mineral ac... |
12700944 | Advancing role of radiolabeled antibodies in the therapy of cancer. | This review focuses on the use of radiolabeled antibodies in the therapy of cancer, termed radioimmunotherapy (RAIT). Basic problems concerned with the choice of antibody, radionuclide, and physiology of the tumor and host are discussed, followed by a review of the pertinent clinical publications of various radioantibo... |
12793896 | 42nd Annual Meeting of the American Society for Cell Biology, San Francisco, California, USA, 14-18 December 2002. | The Annual Meeting of the American Society for Cell Biology (ASCB) is a diverse conference covering all topics in cell biology. While all of the basic biology presented at this meeting may potentially contribute to breast cancer research, there were a significant number of presentations and posters directly pertinent t... |
12629210 | Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants. | The DnaE intein in Synechocystis sp. strain PCC6803 is the first and only naturally split intein that has been identified so far. It is capable of catalyzing a protein trans-splicing mechanism to assemble a mature protein from two separate precursors. Therefore, it is a powerful tool for protein modification and engine... |
12629206 | Mechanics of DNA packaging in viruses. | A new generation of single-molecule experiments has opened up the possibility of reexamining many of the fundamental processes of biochemistry and molecular biology from a unique and quantitative perspective. One technique producing a host of intriguing results is the use of optical tweezers to measure the mechanical f... |
12626766 | Proteasome-dependent, ubiquitin-independent degradation of the Rb family of tumor suppressors by the human cytomegalovirus pp71 protein. | Most of the substrates degraded by the proteasome are marked with polyubiquitin chains. However, there are a limited number of examples of nonubiquitinated proteins that are degraded by the proteasome. Here, we describe the degradation of the retinoblastoma family of tumor suppressor proteins by the proteasome in the a... |
12626752 | Chiral tubule self-assembly from an achiral diynoic lipid. | Tubules possessing microm-scale chiral substructure self-assemble from an achiral isomer of the tubule-forming diynoic phosphatidylcholine, 1,2-bis(10,12-tricosadiynoyl)sn-glycero-3-phosphocholine [DC(8,9)PC], showing that molecular chirality is not essential for tubule formation. CD spectroscopy shows that these struc... |
12626750 | Potent inhibition of scrapie prion replication in cultured cells by bis-acridines. | Prion diseases are characterized by an accumulation of PrP(Sc), a misfolded isoform of the normal cellular prion protein, PrP(C). We previously reported the bioactivity of acridine-based compounds against PrP(Sc) replication in scrapie-infected neuroblastoma cells and now report the improved potency of bis-acridine com... |
20686640 | Dielectrically Addressable Microspheres Engineered Using Self-Assembled Monolayers. | We have used self-assembled monolayer techniques to produce a new class of microspheres with specifically engineered dielectric properties to enable their dielectrophoretic manipulation and identification in microsystems. Dielectrophoresis is an electrokinetic phenomenon that exploits frequency-dependent polarizability... |
19277214 | A Simple 1-D Physical Model for the Crawling Nematode Sperm Cell. | We develop a one-dimensional physical model of the crawling movement of simple cells: The sperm of a nematode, Ascaris suum. The model is based on the assumptions that polymerization and bundling of the cytoskeletal filaments generate the force for extension at the front, and that energy stored in the gel formed from t... |
18968969 | Interaction of oligopyrrole macrocycles with aromatic acids: spectroscopical, quantum chemical and chromatographic aspects. | The study of the behavior of oligopyrrole macrocyclic compounds in solution and covalently bond on silica surface were carried out. For this purpose ultraviolet-visible titrations and chromatography measurements were employed. Interactions of porphyrin and sapphyrin with nitrobenzoic, aminobenzensulfonic, hydroxybenzoi... |
18968933 | Determination of the binding constant for the inclusion complex between procaine hydrochloride and beta-cyclodextrin by capillary electrophoresis. | The apparent electrophoretic mobilities of procaine hydrochloride (mu(i)) in a series of concentration of beta-cyclodextrin were measured directly by capillary electrophoresis technology. A new mathematical treatment method is proposed, which based on the fact that the molar ratio of the inclusion complex was 1:1 estab... |
12737321 | Design and evaluation of a diabody to improve protection against a potent scorpion neurotoxin. | Diabodies are recombinant, dimeric, antibody-based molecules composed of two non-covalently associated single-chain antibody fragments that bind to an antigen in a divalent manner. In an attempt to develop more effective therapeutic molecules against scorpion venoms, we designed a diabody derived from monoclonal antibo... |
12611909 | Trafficking defects in endogenously synthesized cholesterol in fibroblasts, macrophages, hepatocytes, and glial cells from Niemann-Pick type C1 mice. | Niemann-Pick type C1 disease (NPC1) is an inherited neurovisceral lipid storage disorder, hallmarked by the intracellular accumulation of unesterified cholesterol and glycolipids in endocytic organelles. Cells acquire cholesterol through exogenous uptake and endogenous biosynthesis. NPC1 participation in the traffickin... |
12730034 | Re-operation for complicated secondary peritonitis - how to identify patients at risk for persistent sepsis. | <h4>Introduction</h4>There is an ongoing dispute on the benefit of planned relaparotomy for patients with diffuse peritonitis.<h4>Setting</h4>Surgery Department, university hospital.<h4>Patients</h4>145 patients with diffuse peritonitis treated with planned relaparotomy were analysed for APACHE II, MOF- and MODS-score ... |
14724386 | Effects of congenital deafness in the cochlear nuclei of Shaker-2 mice: an ultrastructural analysis of synapse morphology in the endbulbs of Held. | It is well established that manipulation of the sensory environment can significantly alter central auditory system development. For example, congenitally deaf white cats exhibit synaptic alterations in the cochlear nucleus distinct from age-matched, normal hearing controls. The large, axosomatic endings of auditory ne... |
12725700 | Investigation of host-guest stability constants of calix[n]arenes complexes with aromatic molecules by RP-HPLC method. | Under reversed-phase high-performance liquid chromatographic conditions [Spherisorb ODS 1 stationary phase, UV detection at 254 nm, and acetonitrile-dichloromethane-acetic acid-methyl-tert-butylether (84.6/4.5/0.9/10, v/v/v/v) as the mobile phase], adding p-tert-butylcalix[8] (10(-5)-3.10(-5))-[12]arenes (10(-5)-4.10(-... |
12725693 | Chromatographic behavior of ion pair enantiomers of dansyl leucine cyclohexylammonium salt on a beta-cyclodextrin stationary phase and the effect of a competitive-binding mobile phase additive. | The separation of dansyl leucine enantiomers on a beta-cyclodextrin stationary phase is significantly complicated by the association of the amino acid with its cyclohexylammonium counter ion, in a mobile phase of 80:20 (v/v) methanol-water. This produces very unusual chromatography, with two partially superimposed peak... |
12724033 | Antimicrobial activity of beta-lactam antibiotics against clinical pathogens after molecular inclusion in several cyclodextrins. A novel approach to bacterial resistance. | Recognition and uptake by specific cellular receptors and transport systems for cyclodextrins have been demonstrated. Based on this concept, natural and synthetically modified cyclodextrins were used as drug carriers. Several beta-lactam antibiotics were selected and their inclusion complexes with different cyclodextri... |
12723697 | Dosage dependent gene regulation and the compensation of the X chromosome in Drosophila males. | Dosage compensation refers to the phenomenon that despite the difference in copy number of X chromosomes in males and females approximately equal expression results from the sex chromosomes. We describe evidence for a model that dosage compensation is caused by an 'inverse dosage effect' that results from an altered st... |
12722662 | [Binding of aliphatic aldehydes by cornstarch polysaccharides]. | Binding by cryotextured cornstarch of individual aliphatic aldehydes (C6-C10; saturated or unsaturated) and their mixtures from aqueous solutions has been studied using capillary gas chromatography. The amount of compounds absorbed by the cryotextures depended linearly on the concentration of aldehydes in the original ... |
12720279 | Recognition promoted by Zn2+ between phenanthroline bridging polyaza ligands and nucleotides--Zn2+ acts as 'messenger' between the receptor and substrate. | The stability constants of the supramolecular complexes formed between L ((a,b,c,d)) or their Zn(2+) complexes, and adenosine 5'-triphosphate (ATP) in aqueous solution were determined by potentiometric titrations (25 degrees C, I = 0.1 mol dm(-3) KNO(3)). The results show that protonated aliphatic-substituted L (a,d) a... |
12720278 | Therapeutic approaches to superantigen-based diseases: a review. | Superantigens secreted by the bacterial pathogen Staphyloccocus aureus are extremely potent toxins that overstimulate the host immune system by binding to the MHC class II and T cell receptors and activating a large population of T cells. Superantigen infection has been shown to be the causative agents in acute disease... |
12720277 | Comparison of the three-dimensional structures of a human Bence-Jones dimer crystallized on Earth and aboard US Space Shuttle Mission STS-95. | Crystals of a human (Sea) Bence-Jones dimer were produced in a capillary by vapor diffusion under microgravity conditions in the 9 day US Space Shuttle Mission STS-95. In comparison to ground-based experiments, nucleation was facile and spontaneous in space. Appearance of a very large (8 x 1.6 x 1.0 mm) crystal in a sh... |
12720276 | X-ray structural analysis of the ligand-recognition mechanism in the dual-affinity labeling of c-type lysozyme with 2',3'-epoxypropyl beta-glycoside of N-acetyllactosamine. | In spite of the belonging to the same c-type lysozyme family, hen egg-white lysozyme (HEWL) was much less susceptible to the dual-affinity labeling with 2',3'-epoxypropyl beta-glycoside of N-acetyllactosamine (Galbeta1,4GlcNAc-Epo) than human lysozyme (HL). The three-dimensional structures of the HEWL labeled with sing... |
12720275 | Multiple-point adsorption of terbium ions by lead ion templated thermosensitive gel: elucidating recognition of conformation in gel by terbium probe. | Lead ion templated thermosensitive heteropolymer gel which has recognition ability of methacrylate pairs has been synthesized and characterized. The gel consists of a main monomer component, N-isopropylacrylamide (NIPA), responsible for volume phase transition, methacrylic acid (MAA) moieties imprinted as pairs to adso... |
12714446 | Gurmarin suppression of licking responses to sweetener-quinine mixtures in C57BL mice. | Gurmarin (Gur) is a peptide that selectively suppresses responses of the chorda tympani nerve to sweet substances in rats and mice. In the present study, we examined the effect of Gur on behavioral responses to sweet substances in C57BL mice. To accomplish this, we developed a new short-term lick test and measured numb... |
12713218 | Microenvironmental effects in the excited state properties of p-dimethylaminobenzonitrile complexed to alpha- and beta-cyclodextrin. | The steady state and time resolved fluorescence and the triplet-triplet absorption of p-dimethylaminobenzonitrile (DMABN) in presence of alpha- and beta-cyclodextrin (CD) were investigated at various host and guest concentrations and temperatures. The formation of 1:1 and 1:2 DMABN:alpha-CD and 1:1 and 2:2 complexes DM... |
12713215 | Energy transfer within perylene-terrylene dendrimers evidenced by polychromatic transient absorption measurements. | The time dependent spectral properties of a first and a second generation dendrimer with peryleneimide chromophores at the rim and a terrylenediimide chromophore in the core were investigated by time resolved polychromatic transient absorption measurements. The obtained results of the dendritic structures were compared... |
12710621 | Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. | Three novel strains of cold-adapted bacteria, ST-82T, ST-10 and ST-92, were isolated from freshwater sediments. These three isolates were very similar to each other in phenotypic and chemotaxonomic traits, as well as in 16S rDNA sequence. The strains were Gram-negative, elongated filament-like rods that formed bright y... |
12708763 | Modification of polymer surfaces: optimization of approaches. | Modification of polymer surfaces to achieve a surface with enhanced compatibility is an important means of obtaining improved biomaterials. Techniques are available for altering the hydrophilicity or charge of a surface, attaching macromolecules or attempting to resemble cell membranes. Relevant to the clinical success... |
12705382 | Electrochemical recognition of analytes using quaternary ammonium binaphthyl salts. | The electrooxidation of quaternary ammonium binaphthyl salts is shown to proceed by a quasi-reversible one-electron process. The oxidation of an aza-crown ether substituted binaphthyl salt is affected by the presence of lithium and sodium ions in solution and there is shown to be a linear relationship between the limit... |
12703828 | Synthesis of dendrimers with multifunctional periphery using an ABB' monomer. | A new methodology using an ABB' monomer for synthesizing dendrons with multiple functionalities in the periphery is described. |
12703806 | Electrochemically tuneable hydrogen bonding interactions between a phenyl-urea terminated dendrimer and phenanthrenequinone. | Electrochemically tuneable hydrogen bonding interactions are described between a phenyl-urea terminated dendrimer and phenanthrenequinone. |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.