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12703800 | Construction of extended networks with a trimeric pyrazole synthon. | A H-bonded pyrazole trimeric motif drives the self-association of bifunctional tetramethyl-4,4'-bipyrazole, yielding highly interpenetrated 3D frameworks and allowing the engineering by design of a novel supramolecular isomer. |
12703771 | A novel synthetic strategy for hexanuclear supramolecular architectures. | Hexanuclear cage complexes [M6L6X](X)5 [M = Cu(I), Ag(I); L = 6,6'-bis(4-ethynylpyridine)2,2'-bipyridine; X = BF4-, SbF6-] have been prepared using a self-assembly approach; these architectures encapsulate anions in the solid-state and are fluxional in solution. |
12703552 | Morphological study of disordered myelination and the degeneration of nerve fibers in the spinal cord of mice lacking complex gangliosides. | Gangliosides, a family of glycosphingolipids that contain sialic acid, are abundant on the neuronal cell membranes, but their precise functions in the central nervous system remain largely undefined. In a previous study of GalNAc-T(-/-) mice engineered to lack beta1,4-N-acetylgalactos-aminyltransferase (GM2/GD2 synthas... |
12702802 | Diversity in the SH2 domain family phosphotyrosyl peptide binding site. | Src homology 2 (SH2) domains are approximately 100 residue phosphotyrosyl peptide binding modules found in signalling proteins and are important targets for therapeutic intervention. The peptide binding site is evolutionarily well conserved, particularly at the two major binding pockets, pTyr and pTyr + 3. We present a... |
12697262 | Determination of plasma membrane fluidity with a fluorescent analogue of sphingomyelin by FRAP measurement using a standard confocal microscope. | Membrane perturbing effects have been described in neurodegenerative process like Alzheimer's disease and prion disorders. For example, non fibrillar amyloid-beta peptides (Abeta) implicated in Alzheimer's disease may exert its toxicity via membrane perturbation. Membrane organisation can be evaluated by its influence ... |
12685606 | Multivariate optimisation of a cyclodextrin-assisted-capillary zone electrophoretic method for the separation of torasemide and its metabolites. | In this work, a rapid cyclodextrin-assisted capillary electrophoretic method is developed for the separation of the diuretic torasemide and three of its metabolites. Both fractional factorial and central composite designs were employed to optimise the separation method. The factors studied were pH, concentration of met... |
12685590 | Determination of orotic acid in urine by capillary zone electrophoresis in tandem-coupled columns with diode array detection. | Analytical potentialities of capillary zone electrophoresis in the separation system with tandem-coupled columns to the spectral identification and determination of orotic acid (OA) in urine by diode array detection (DAD), coupled to the separation system via optical fibers, were investigated. A very significant "in-co... |
12685587 | Enantioseparation of basic pharmaceutical compounds by capillary electrophoresis using sulfated cyclodextrins. Application to E-6006, a novel antidepressant. | In this study, a chiral capillary electrophoresis method was optimized and validated for E-6006, a thienylpyrazolylethanamine derivative (pKa 8.9). Enantioselectivity of neutral and anionic cyclodextrins (CDs) was evaluated at acid pH (3), obtaining cathodic and anodic migration, respectively. Hydroxypropyl-beta-CD, ca... |
12685584 | Electrophoretic enantiomer separations at high pH using the new, single-isomer octakis(2,3-dimethyl-6-O-sulfo)-gamma-cyclodextrin as chiral resolving agent. | The latest, single-isomer, sulfated gamma-cyclodextrin, the sodium salt of octakis(2,3-dimethyl-6-O-sulfo)-gamma-cyclodextrin that is stable in basic media was used to separate the enantiomers of neutral, weak acid and weak base analytes by capillary electrophoresis in high pH aqueous background electrolytes. The effec... |
12683106 | A comparison of potential molecular wires as components for molecular electronics. | The field of electronics using single-molecule components has recently received much attention as a possible new design concept for the continued miniturisation of electronics. Molecular wires are the conceptually simplest components of such electronic systems and several different compound types have been used to prod... |
12678563 | Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis. | Modification of starch biosynthesis pathways holds an enormous potential for tailoring granules or polymers with new functionalities. In this study, we explored the possibility of engineering artificial granule-bound proteins, which can be incorporated in the granule during biosynthesis. The starch-binding domain (SBD)... |
12673413 | Clinical study of Japanese spotted fever and its aggravating factors. | Twenty-eight patients with Japanese spotted fever were clinically investigated. The diagnosis was determined by confirming an increase of specific antibody. All patients were treated with minocycline, and all recovered, excluding one patient with a fulminant course. Fever and exanthema were observed in all patients, an... |
12663529 | A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin. | Using the set of Saccharomyces cerevisiae mutants individually deleted for 5718 yeast genes, we screened for altered sensitivity to the antifungal protein, K1 killer toxin, that binds to a cell wall beta-glucan receptor and subsequently forms lethal pores in the plasma membrane. Mutations in 268 genes, including 42 in ... |
12660112 | Hydration-optimized oriented phospholipid bilayer samples for solid-state NMR structural studies of membrane proteins. | The preparation of oriented, hydration-optimized lipid bilayer samples, for NMR structure determination of membrane proteins, is described. The samples consist of planar phospholipid bilayers, containing membrane proteins, that are oriented on single pairs of glass slides, and are placed in the coil of the NMR probe wi... |
12659195 | Application of cyclam-capped beta-cyclodextrin-bonded silica particles as a chiral stationary phase in capillary electrochromatography for enantiomeric separations. | Two novel types of substituted cyclam-capped beta-cyclodextrin (beta-CD)-bonded silica particles have been prepared and used as chiral stationary phases in capillary electrochromatography (CEC). The two stationary phases have a chiral selector with three recognition sites: beta-CD, cyclam, and the latter's sidearm. The... |
12657692 | Estrogen stimulates postsynaptic density-95 rapid protein synthesis via the Akt/protein kinase B pathway. | Estrogens induce synaptogenesis in the CA1 region of the dorsal hippocampus during the estrous cycle of the female rat. Functional consequences of such estrogen-mediated synaptogenesis include cyclic changes in neurotransmission and memory. At the molecular level, estrogen stimulates the rapid activation of specific si... |
12658701 | Chiral separation of 3,4-methylenedioxymeth- amphetamine and related compounds in clandestine tablets and urine samples by capillary electrophoresis/fluorescence spectroscopy. | The R-(-)- and S-(+)-isomers of 3,4-methylenedioxymethamphetamine (MDMA) and its metabolite 3,4-methylenedioxyamphetamine (MDA) were prepared, identified by gas chromatography/mass spectrometry (GC/MS) and then used as standards in a series of capillary electrophoresis (CE) experiments. Using these R-(-)- and S-(+)-iso... |
12658700 | Capillary electrophoretic separation of glutamate enantiomers in neural samples. | A micellar electrokinetic chromatographic (MEKC) separation of glutamate (Glu) enantiomers fluorescently tagged with naphthalene-2,3-dicarboxaldehyde (NDA) is described. Chiral selectors tested included alpha-, beta-, and gamma-cyclodextrins, modified cyclodextrins, D-glucosamine, sucrose, taurocholate, and their binar... |
12658699 | Comparative enantioseparations with native beta-cyclodextrin, randomly acetylated beta-cyclodextrin and heptakis-(2,3-di-O-acetyl)-beta-cyclodextrin in capillary electrophoresis. | Comparative enantioseparations were performed with three neutral cyclodextrins (CDs) in capillary electrophoresis (CE). In particular, native beta-CD was compared with single component heptakis(2,3-di-O-acetyl)-beta-CD (HDA-beta-CD) and randomly acetylated beta-CD (Ac-beta-CD) with the emphasis on the enantiomer migrat... |
12658697 | Migration order of dipeptide and tripeptide enantiomers in the presence of single isomer and randomly sulfated cyclodextrins as a function of pH. | The present study was conducted in order to evaluate the cyclodextrin (CD)-mediated chiral separation of peptide enantiomers as uncharged analytes at pH 5.3 using randomly sulfated beta-cyclodextrin, heptakis-6-sulfato-beta-CD and heptakis-(2,3-diacetyl-6-sulfato)-beta-CD as chiral selectors. Although less effective co... |
12651138 | Interfacial interactions between amphiphilic cyclodextrins and physiologically relevant cations. | The compression isotherms of a series of amphiphilic cyclodextrins, formed (a) by acylation at the secondary hydroxyl face and (b) by acylation accompanied by varying degrees of sulfatation (DS) at the primary hydroxyl face (DS=0, 4, and 7), have been studied on subphases of pure water and of water containing NaCl, KCl... |
12652330 | Separate and combined effects of local and continuous intravenous administration of beta-cyclodextrin tetradecasulfate on intimal hyperplasia after angioplasty in porcine coronary arteries. | <h4>Background</h4>Beta-Cyclodextrin tetradecasulfate binds fibroblast growth factors and possesses anticoagulant properties. This study was designed to assess the separate and combined effects of local intramural delivery and intravenous administration of beta-cyclodextrin tetrade-casulfate on neointimal formation and... |
12650254 | Monitoring dediazoniation product formation by high-performance liquid chromatography after derivatization. | A derivatization protocol that exploits the rapid reaction between arenediazonium ions and a suitable coupling agent followed by high-performance liquid chromatography analyses of the reaction mixture was employed to determine the product distribution, the rate constants for product formation and the association consta... |
12648199 | Anti-inflammatory cytokine response and the development of multiple organ failure in severe sepsis. | <h4>Background</h4>The production of proinflammatory cytokines activates the systemic inflammatory response in sepsis. Patients also develop a compensatory anti-inflammatory reaction, which may have an important down-regulatory effect on the overactive inflammation. However, the role of this anti-inflammatory response ... |
12644993 | Macromolecular assembly of Helicobacter pylori urease investigated by mass spectrometry. | The supramolecular assembly of Helicobacter pylori urease was studied by nanoflow electrospray ionization orthogonal time-of-flight mass spectrometry. The measured molecular mass of the urease complex of 1.06 MDa corresponds to a dodecameric (alphabeta)(12) assembly of urease alpha (26 kDa) and beta (61 kDa) subunits. ... |
12643725 | Complexity of coordinative bonding in thallium(I) anthranilates and salicylates. | An inventory of the structural chemistry of thallium(I) shows many unexpected, almost random coordination numbers and coordination geometries that appear erratic and inconsistent. This nonstandard behavior is often ascribed to the specific lone-pair characteristics originating from relativistic effects. To provide data... |
12643714 | Supramolecular pseudorotaxane polymers from complementary pairs of homoditopic molecules. | Self-assembly of supramolecular pseudorotaxane polymers from complementary homoditopic building blocks comprised of bis(dibenzo-24-crown-8) esters derived from the hydroxymethyl crown ether and aliphatic diacid chlorides (CxC, x = number of methylene units in the diacid segment) and 1,10-bis[p-(benzylammoniomethyl)phen... |
12643707 | Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. | Number- and size-controlled macromolecular associations are common in biology with viruses as a typical example. We report here a novel example of artificial viruses, in which the double-helical DNA is coated with 4-nm sized neutral glycocluster nanoparticles (GNPs) with a coating stoichiometry of approximately 2 GNPs ... |
12643702 | Direct preparation of nanoporous carbon by nanocasting. | Nanoporous carbon with narrow pore size distribution was prepared via a nanocasting technique by direct carbonization of cyclodextrin-silica organic-inorganic hybrid composite. The obtained carbon exhibited very high BET surface area and high pore volume. |
12643692 | Cross-linked dendrimer hosts containing reporter groups for amine guests. | The integration of a chromogenic reporter group into the recently reported (Zimmerman, S. C.; Wendland, M. S.; Rakow, N. A.; Zharov, I.; Suslick, K. S. Nature 2002, 418, 399-403) monomolecular imprinting approach is described. The resulting highly cross-linked, macromolecular hosts show rapid, selective, high affinity,... |
12643673 | Partial purification of latent persimmon fruit polyphenol oxidase. | Persimmon fruit polyphenol oxidase (PPO) was partially purified using a combination of phase partitioning with Triton X-114 and ammonium sulfate fractionation between 50 and 75%. The enzyme, which showed both monophenolase and diphenolase activities, was partially purified in a latent form and could be optimally activa... |
12645023 | Tetra- and octalactam macrocycles and catenanes with exocyclic metal coordination sites: versatile building blocks for supramolecular chemistry. | The synthesis of a new tetralactam macrocycle and the simultaneous formation of catenanes and larger octalactam macrocycles is reported. These species bear 2,2'-biquinoline moieties suitably positioned to bind a metal center at the outer periphery of the macrocycles. (1)H NMR chemical shifts permit the unambiguous dist... |
12645021 | Characterization of the 310-helix in model peptides by HRMAS NMR spectroscopy. | A tetra- and a hepta-homopeptide from the C(alpha)-tetrasubstituted Aib (alpha-aminoisobutyric acid) residue were covalently linked to the POEPOP resin by the fragment-condensation approach. The conformational preferences of the two model peptides were determined for the first time on a solid support by means of high-r... |
12644376 | Macrocycle conformation and self-inclusion phenomena in octakis(3-O-butanoyl-2,6-di-O-pentyl)-gamma-cyclodextrin (Lipodex E) by NMR spectroscopy and molecular dynamics. | Octakis(3-O-butanoyl-2,6-di-O-pentyl)-gamma-cyclodextrin (Lipodex E) is a lipophilic chiral selector successfully used for the enantioselective gas chromatographic separation of a multitude of racemic analytes. NMR data (13C chemical shifts, 3J(HH), rotating frame NOEs (ROEs)) and molecular dynamics (MD) simulations po... |
12643755 | Generation of a functional monomolecular protein lattice consisting of an s-layer fusion protein comprising the variable domain of a camel heavy chain antibody. | Crystalline bacterial cell surface layer (S-layer) proteins are composed of a single protein or glycoprotein species. Isolated S-layer subunits frequently recrystallize into monomolecular protein lattices on various types of solid supports. For generating a functional protein lattice, a chimeric protein was constructed... |
12643749 | Macromolecular MRI contrast agents with small dendrimers: pharmacokinetic differences between sizes and cores. | Large macromolecular MRI contrast agents with albumin or dendrimer cores are useful for imaging blood vessels. However, their prolonged retention is a major limitation for clinical use. Although smaller dendrimer-based MRI contrast agents are more quickly excreted by the kidneys, they are also able to visualize vascula... |
12643744 | In vitro and in vivo gene transfer by an optimized alpha-cyclodextrin conjugate with polyamidoamine dendrimer. | The purpose of the present study is to optimize the structure of the polyamidoamine starburst dendrimer (dendrimer) conjugate with alpha-cyclodextrin (alpha-CDE conjugate) as a nonviral vector. alpha-CDE conjugates of dendrimer (generation 3, G3) with various average degrees of substitution (DS) of alpha-CyD of 1.1, 2.... |
12645054 | Interactions with aromatic rings in chemical and biological recognition. | Intermolecular interactions involving aromatic rings are key processes in both chemical and biological recognition. Their understanding is essential for rational drug design and lead optimization in medicinal chemistry. Different approaches-biological studies, molecular recognition studies with artificial receptors, cr... |
12642617 | Formation of filopodia-like bundles in vitro from a dendritic network. | We report the development and characterization of an in vitro system for the formation of filopodia-like bundles. Beads coated with actin-related protein 2/3 (Arp2/3)-activating proteins can induce two distinct types of actin organization in cytoplasmic extracts: (1) comet tails or clouds displaying a dendritic array o... |
12642616 | A neurotoxic peripherin splice variant in a mouse model of ALS. | Peripherin, a neuronal intermediate filament (nIF) protein found associated with pathological aggregates in motor neurons of patients with amyotrophic lateral sclerosis (ALS) and of transgenic mice overexpressing mutant superoxide dismutase-1 (SOD1G37R), induces the selective degeneration of motor neurons when overexpr... |
12642615 | Drosophila paramyosin is important for myoblast fusion and essential for myofibril formation. | Paramyosin is a major structural protein of thick filaments in invertebrate muscles. Coiled-coil dimers of paramyosin form a paracrystalline core of these filaments, and the motor protein myosin is arranged on the core surface. To investigate the function of paramyosin in myofibril assembly and muscle contraction, we f... |
12642609 | Axonal transport of membranous and nonmembranous cargoes: a unified perspective. | Membranous and nonmembranous cargoes are transported along axons in the fast and slow components of axonal transport, respectively. Recent observations on the movement of cytoskeletal polymers in axons suggest that slow axonal transport is generated by fast motors and that the slow rate is due to rapid movements interr... |
12642377 | Cannabinoid receptor-independent inhibition by cannabinoid agonists of the peripheral 5-HT3 receptor-mediated von Bezold-Jarisch reflex. | (1) On the basis of previous findings that cannabinoids inhibit the function of human and rat 5-HT(3) receptors in vitro, we investigated whether cannabinoid receptor agonists also modulate the activity of the rat peripheral 5-HT(3) receptors on the terminals of cardiopulmonary afferent C-fibres in vivo. (2) In urethan... |
12641284 | Preparative-scale isoelectric trapping enantiomer separations. | The new Gradiflow BF200 IET unit, developed for isoelectric trapping protein separations has been modified and used to carry out preparative-scale enantiomer separations. Hydroxypropyl beta-cyclodextrin was used as the chiral resolving agent to induce an isoelectric point difference between the enantiomers. Three isoel... |
12639155 | Mixed dinitrogen-organocyanamide complexes of molybdenum(0) and their protic conversion into hydrazide and amidoazavinylidene derivatives. | Organocyanamides, Ntbd1;CNR(2) (R = Me or Et), react with trans-[Mo(N(2))(2)(dppe)(2)] (1, dppe = Ph(2)PCH(2)CH(2)PPh(2)), in THF, to give the first mixed molybdenum dinitrogen-cyanamide complexes trans-[Mo(N(2))(NCNR(2))(dppe)(2)] (R = Me 2a or Et 2b) which are selectively protonated at N(2) by HBF(4) to yield the hyd... |
12639144 | Porphyrins linked directly to the 5,5' positions of 2,2'-bipyridine: a new supramolecular building block and switch. | The synthesis and coordination chemistry of two porphyrin dimers linked either at the 5,5' or the 4,4' positions of 2,2'-bipyridine are described. These compounds, which may serve a molecular tectons for the constructions of a variety of supramolecular arrays of diverse function, reveal that the ground- and excited-sta... |
12639130 | Self-assembly in palladium(II) and platinum(II) chemistry: the biomimetic approach. | The self-assembly of complex cationic structures by combination of cis-blocked square planar palladium(II) or platinum(II) units with bis(pyridyl) ligands having bridging amide units has been investigated. The reactions have yielded dimers, molecular triangles, and polymers depending primarily on the geometry of the bi... |
12639120 | Zinc(II)-templated synthesis of a [2]-catenane consisting of a 2,2',6',2' '-terpyridine-incorporating cycle and a 1,10-phenanthroline-containing ring. | A nonsymmetrical [2]-catenane has been synthesized, with a 5-coordinated metal center (Zn(2+)) as template. One of the two rings contains a terdentate ligand (2,2',6',2' '-terpyridine) and the other one incorporates a bidentate chelate (1,10-phenanthroline). The first ring was prepared separately and, subsequently, Zn(... |
12636418 | Molecular recognition of fluoride anion: benzene-based tripodal imidazolium receptor. | A benzene-based tripodal imidazolium receptor utilizing the strong (C-H)(+)...X(-) hydrogen bonding interaction between imidazolium moieties and halide anions is extensively investigated both theoretically and experimentally. Ab initio calculations predict that this receptor has a very high affinity for fluoride ion (F... |
12636406 | Crowned dendrimers: pH-responsive pseudorotaxane formation. | With the end goal of incorporating the unique structural and physical properties of dendrimers into supramolecular assemblies, bis(m-phenylene)-32-crown-10-functionalized poly(propyleneimine) dendrimers of the first and third generations have been synthesized and their interaction with paraquat diol has been investigat... |
12636398 | Synthesis of all stereoisomers of sulfinylcalix[4]arenes. | All four stereoisomers of p-tert-butylsulfinylcalix[4]arene [4(rccc), 4(rcct), 4(rctt), and 4(rtct)], arising from the disposition of the four sulfinyl groups with respect to the mean plane of the four bridging sulfur atoms, have been prepared via oxidation of p-tert-butylthiacalix[4]arene (2) or its tetra-O-benzyl eth... |
12636396 | Concerted rearrangement versus heterolytic cleavage in anionic [2,3]- and [3,3]-sigmatropic shifts. A DFT study of relationships among anion stabilities, mechanisms, and rates. | The anionic [2,3] sigmatropic Wittig rearrangements of deprotonated 4-hetera-1-pentenes and the anionic [3,3] sigmatropic Cope rearrangements of 3-substituted-1,5-hexadienes were explored by using density functional theory calculations. While the deprotonated anionic 3-hydroxy-1,5 hexadiene (2a), 3-thiohydroxy-1,5-hexa... |
12636382 | Synthetic studies toward sarain A. Formation of the western macrocyclic ring. | Starting with the tricyclic core 2b, annulation to form the 13-membered western ring of sarain A has been achieved to afford the macrocycle 30a by initial construction of the sterically congested quaternary center at C-3, followed by elaboration of the C-3 side-chain and ring-closing olefin metathesis. Also included is... |
12636376 | An approach to long and unsubstituted molecular wires: synthesis of redox-active, cationic phenylethynyl oligomers designed for self-assembled monolayers. | Various oligo(phenyleneethynylene)s (OPEs) have been synthesized in the past, as they are considered as prototypes of molecular wires. When the oligomers are capped by a redox site at one end and a thiol at the other end, the resulting molecules can be grafted as a self-assembled monolayer on a gold electrode and fully... |
12637105 | Phase solubility studies of pure (-)-alpha-bisabolol and camomile essential oil with beta-cyclodextrin. | (-)-Alpha-bisabolol was found to form an inclusion complex with beta-cyclodextrin (beta-CD) in solution as well as in the solid state. To investigate molecular associations of beta-CD with pure (-)-alpha-bisabolol or (-)-alpha-bisabolol as a component of camomile essential oil, phase solubility studies were undertaken.... |
12637096 | Complex formation of sericoside with hydrophilic cyclodextrins: improvement of solubility and skin penetration in topical emulsion based formulations. | The main objective of this study was to devise novel methods for improving the solubility of the anti-inflammatory triterpenoid sericoside, the main component of Terminalia sericea extract, thus enabling its incorporation into topical formulations. Sericoside was stabilized by complex formation with hydrophilic derivat... |
12637094 | A study of the freeze-drying conditions of calixarene based solid lipid nanoparticles. | In this note, we report a study of cryoprotectant carbohydrate (glucose, fructose, mannose and maltose) effects on the reconstitution of calixarene based solid lipid nanoparticle (SLN) suspensions after freeze-drying, studied by atomic force microscopy and photon correlation spectroscopy. All carbohydrates tested showe... |
12637090 | Improvement of pulmonary absorption of cyclopeptide FK224 in rats by co-formulating with beta-cyclodextrin. | FK224 is a cyclopeptide drug with a low aqueous solubility. Following oral administration to rats, poor absorption was observed due to proteolysis in the gastrointestinal tract. The objective of this study was to investigate the effect of the pulmonary route on the systemic absorption of FK224 in comparison with other ... |
12633728 | Luminescence behavior of a water soluble calix[4]arene derivative complex with terbium ion(III) in gelation solution. | The complexation luminescence behavior of a water soluble calix[4]arene derivative, 5,11,17,23-tetra-sulfonate-25,26,27,28-tetra-carboxymethoxycalix[4]arene (L) with lanthanoid ion (Tb(3+)) has been investigated in gelation solution at 25 degrees C by using UV-vis and fluorescence spectra. The results obtained indicate... |
12633721 | Molecular recognition of 7-(2-octadecyloxycarbonylethyl)guanine to cytidine at the air/water interface and LB film studied by Fourier transform infrared spectroscopy. | Monolayer behavior of a nucleolipid amphiphile, 7-(2-octadecyloxycarbonylethyl)guanine (ODCG), on aqueous cytidine solution was investigated by means of surface-molecular area (pi-A) isotherms. It indicates that molecular recognition by hydrogen bonding is present between ODCG monolayer and the cytidine in subphase. Th... |
12633708 | Spectrofluorimetric study of host-guest complexation of ibuprofen with beta-cyclodextrin and its analytical application. | The characteristics of host-guest complexation between beta-cyclodextrin (beta-CD) and two forms of ibuprofen (protonated and deprotonated) were investigated by fluorescence spectrometry. 1:1 stoichiometries for both complexes were established and their association constants at different temperatures were calculated by... |
12634062 | Reversible amyloid formation by the p53 tetramerization domain and a cancer-associated mutant. | The tetramerization domain for wild-type p53 (p53tet-wt) and a p53 mutant, R337H (p53tet-R337H), associated with adrenocortical carcinoma (ACC) in children, can be converted from the soluble native state to amyloid-like fibrils under certain conditions. Circular dichroism, Fourier transform infrared spectroscopy and st... |
12632484 | Peptidergic innervation of human esophageal and cardiac carcinoma. | <h4>Aim</h4>To investigate the distribution of neuropeptide-immunoreactive nerve fibers in esophageal and cardiac carcinoma as well as their relationship with tumor cells so as to explore if there is nerve innervation in esophageal and cardiac carcinoma.<h4>Methods</h4>Esophageal and cardiac carcinoma specimens were co... |
12631725 | A dynamin-cortactin-Arp2/3 complex mediates actin reorganization in growth factor-stimulated cells. | The mechanisms by which mammalian cells remodel the actin cytoskeleton in response to motogenic stimuli are complex and a topic of intense study. Dynamin 2 (Dyn2) is a large GTPase that interacts directly with several actin binding proteins, including cortactin. In this study, we demonstrate that Dyn2 and cortactin fun... |
12631724 | Dynamin participates in focal extracellular matrix degradation by invasive cells. | The degradation of extracellular matrix (ECM) by matrix metalloproteases is crucial in physiological and pathological cell invasion alike. Degradation occurs at specific sites where invasive cells make contact with the ECM via specialized plasma membrane protrusions termed invadopodia. Herein, we show that the dynamin ... |
12631723 | Myo3A, one of two class III myosin genes expressed in vertebrate retina, is localized to the calycal processes of rod and cone photoreceptors and is expressed in the sacculus. | The striped bass has two retina-expressed class III myosin genes, each composed of a kinase, motor, and tail domain. We report the cloning, sequence analysis, and expression patterns of the long (Myo3A) and short (Myo3B) class III myosins, as well as cellular localization and biochemical characterization of the long is... |
12631719 | Specification of actin filament function and molecular composition by tropomyosin isoforms. | The specific functions of greater than 40 vertebrate nonmuscle tropomyosins (Tms) are poorly understood. In this article we have tested the ability of two Tm isoforms, TmBr3 and the human homologue of Tm5 (hTM5(NM1)), to regulate actin filament function. We found that these Tms can differentially alter actin filament o... |
12630879 | Cavity-directed synthesis within a self-assembled coordination cage: highly selective [2 + 2] cross-photodimerization of olefins. | Herein reported is the highly selective [2 + 2] cross-photodimerization of olefins within a self-assembled coordination cage that acts as a molecular flask in an aqueous medium. An M(6)L(4) coordination nanocage that self-assembles from six Pd(II) complexes and four tridentate ligands accommodates two different kinds o... |
12632428 | Identification and characterization of a peptide mimetic that may detect a species of disease-associated anticardiolipin antibodies in patients with the antiphospholipid syndrome. | <h4>Objective</h4>To test the feasibility of applying a mimetic (specific for a patient-derived prothrombotic anticardiolipin antibody [aCL]) to study the homologous, disease-associated aCL in patients with antiphospholipid syndrome (APS).<h4>Methods</h4>We used the CL15 monoclonal aCL to screen 17 phage-display peptid... |
12629187 | Perinatal neurosteroid levels influence GABAergic interneuron localization in adult rat prefrontal cortex. | Neurosteroids are a class of steroids synthesized de novo in the brain, several of which are potent modulators of GABA(A) receptor function. In developing brain GABA(A) receptor, stimulation plays a trophic role. Cortical levels of the GABAergic neurosteroid 3alpha-hydroxy-5alpha-pregnan-20-one (3alpha,5alpha-THP) vary... |
12629171 | Interaction between liprin-alpha and GIT1 is required for AMPA receptor targeting. | Liprin-alpha is a multidomain protein that interacts with the LAR family of receptor protein tyrosine phosphatases and the GRIP/ABP family of AMPA receptor-interacting proteins. Previous studies have indicated that liprin-alpha regulates the development of presynaptic active zones and that the association of liprin-alp... |
12628256 | Thermodynamics of a designed protein catenane. | Topological linking of proteins is a new approach for stabilizing and controlling the oligomerization state of proteins that fold in an interwined manner. The recent design of a backbone cyclized protein catenane based on the p53tet domain suggested that topological cross-linking provided increased stability against th... |
12628933 | Protein motion from non-specific to specific DNA by three-dimensional routes aided by supercoiling. | DNA-binding proteins are generally thought to locate their target sites by first associating with the DNA at random and then translocating to the specific site by one-dimensional (1D) diffusion along the DNA. We report here that non-specific DNA conveys proteins to their target sites just as well when held near the tar... |
12628932 | The complex between a four-way DNA junction and T7 endonuclease I. | The junction-resolving enzyme endonuclease I is selective for the structure of the DNA four-way (Holliday) junction. The enzyme binds to a four-way junction in two possible orientations, with a 4:1 ratio, opening the DNA structure at the centre and changing the global structure into a 90 degrees cross of approximately ... |
12628479 | Statin effects on cholesterol micro-domains in brain plasma membranes. | Recent epidemiological studies revealed inhibitors of the hydroxymethylglutaryl-coenzyme A reductase, so-called statins, to be effective in lowering the prevalence of Alzheimer's disease (AD). In vitro, statins strongly reduced the cellular amyloid beta-protein load by modulating the processing of the amyloid beta prec... |
12627638 | Role of metalloporphyrin core metals in the mediated reductive dechlorination of tetrachloroethylene. | A promising approach to abiotically dechlorinate a variety of chlorinated organic contaminants under reducing conditions is to utilize porphyrins or other tetrapyrrole macrocycles as electron transfer mediators/shuttles for catalyzing their reduction. In this study, various experimental approaches were used to elucidat... |
12627395 | Structural evidence for common functions and ancestry of the reovirus and adenovirus attachment proteins. | The crystal structure of the reovirus attachment protein, sigma1, reveals a fibre-like structure that is remarkably similar to that of the adenovirus attachment protein, fibre. Both proteins are trimers with head-and-tail morphology. They share unique domain structures and functional properties including defined region... |
12626715 | An NMR study on the interaction of Escherichia coli DinI with RecA-ssDNA complexes. | The SOS response, a set of cellular phenomena exhibited by eubacteria, is initiated by various causes that include DNA damage-induced replication arrest, and is positively regulated by the co- protease activity of RecA. Escherichia coli DinI, a LexA-regulated SOS gene product, shuts off the initiation of the SOS respon... |
12626704 | Crystal structure of the Escherichia coli dcm very-short-patch DNA repair endonuclease bound to its reaction product-site in a DNA superhelix. | Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising by deamination of 5-methyl-cytosines in specific regulatory sequences. We have now determined the structure of the archetypal dcm-Vsr endonuclease from Escherichia coli bound to... |
12626685 | Bacteriophage T4 genome. | Phage T4 has provided countless contributions to the paradigms of genetics and biochemistry. Its complete genome sequence of 168,903 bp encodes about 300 gene products. T4 biology and its genomic sequence provide the best-understood model for modern functional genomics and proteomics. Variations on gene expression, inc... |
12626684 | Two families of mechanosensitive channel proteins. | Mechanosensitive (MS) channels that provide protection against hypoosmotic shock are found in the membranes of organisms from the three domains of life: bacteria, archaea, and eucarya. Two families of ubiquitous MS channels are recognized, and these have been designated the MscL and MscS families. A high-resolution X-r... |
12627443 | Separation of peptides and proteins by capillary electrophoresis using acidic buffers containing tetraalkylammonium cations and cyclodextrins. | A method for improving separations of peptides and other positively charged species in capillary zone electrophoresis with untreated capillaries using acidic buffers containing tetraalkylammonium cations is described. Tetramethylammonium and tetrabutylammonium cations dynamically modify the capillary surface, leading t... |
12626455 | Serological cross-reactivities between antibodies to simian virus 40, BK virus, and JC virus assessed by virus-like-particle-based enzyme immunoassays. | Enzyme immunoassays (EIAs) for detection of serum antibodies to simian virus 40 (SV40), BK virus (BKV), and JC virus (JCV) were developed by using virus-like-particles (VLPs) produced in insect cells from recombinant baculoviruses expressing the VP1 protein of the respective virus. Rhesus macaque sera with neutralizing... |
12626451 | Contribution of epitope specificity to the binding of monoclonal antibodies to the capsule of Cryptococcus neoformans and the soluble form of its major polysaccharide, glucuronoxylomannan. | Incubation of encapsulated cryptococci with monoclonal antibodies (MAbs) specific for glucuronoxylomannan (GXM), the major capsular polysaccharide of Cryptococcus neoformans, produces two distinct capsular quellung-type reactions termed rim and puffy. The type of capsular reaction that occurs is determined by the epito... |
12625746 | Use of small-angle neutron scattering to study tubulin polymers. | Small-angle neutron scattering has been used to examine taxol-stabilized microtubules and other tubulin samples in both H(2)O and D(2)O buffers. Measurements were made at pH/pD values between 6.0 and 7.8, and observed scattered intensities, I(Q), have been interpreted in terms of multicomponent models of microtubules a... |
12623195 | Effect of pH and sodium chloride on the strength and selectivity of the interaction of gamma [correction for tau] -cyclodextrin with some antisense nucleosides. | The influence of pH and the concentration of sodium chloride on the strength and selectivity of the interaction of twelve 8-substituted-2'-deoxyadenosine and sixteen 5-substituted-2'-deoxyuridine derivatives with gamma-cyclodextrin (GCD) have been studied by the spectral mapping technique (SPM). The potency values of t... |
12623123 | Tumor necrosis factor alpha-induced apoptosis in astrocytes is prevented by the activation of P2Y6, but not P2Y4 nucleotide receptors. | The physiological role of the uracil nucleotide-preferring P2Y(6) and P2Y(4) receptors is still unclear, although they are widely distributed in various tissues. In an effort to identify their biological functions, we found that activation by UDP of the rat P2Y(6) receptor expressed in 1321N1 human astrocytes significa... |
12620248 | Pheromonal stimulation of spawning release of gametes by gonadotropin releasing hormone in the chiton, Mopalia sp. | The chiton Mopalia sp., a mollusc, was exposed to various dilutions of gonadotropin releasing hormone (GnRH) in sea water to determine whether this peptide is capable of acting as a pheromone that could stimulate release of ripe gametes (spawning). Two of the peptides, lamprey GnRH-1 and tunicate GnRH-2, had this actio... |
12620848 | Transcriptional cross-regulation of the catechol and protocatechuate branches of the beta-ketoadipate pathway contributes to carbon source-dependent expression of the Acinetobacter sp. strain ADP1 pobA gene. | Transcriptional control of carbon source preferences by Acinetobacter sp. strain ADP1 was assessed with a pobA::lacZ fusion during growth on alternative substrates. The pobA-encoded enzyme catalyzes the first step in the degradation of 4-hydroxybenzoate, a compound consumed rapidly as a sole carbon source. If additiona... |
12619931 | Functional domains for amelogenin revealed by compound genetic defects. | We have previously used the yeast two-hybrid assay and multiple in vitro methodologies to show that amelogenin undergoes self-assembly involving two domains (A and B). Using transgenic animals, we show that unique enamel phenotypes result from disruptions to either the A- or B-domain, supporting the role of amelogenin ... |
12618518 | Chronic myelogenous leukemia shapes host immunity by selective deletion of high-avidity leukemia-specific T cells. | We have shown that cytotoxic T lymphocytes specific for PR1, an HLA-A2-restricted nonopeptide derived from proteinase 3, kill leukemia cells and may contribute to the elimination of chronic myelogenous leukemia (CML) after treatment with IFN or allogeneic bone marrow transplant. Some patients with persistent disease al... |
12617673 | Porous lanthanide-organic frameworks: synthesis, characterization, and unprecedented gas adsorption properties. | The reactions of Ln(NO(3))(3) (Ln = La, Er) with 1,4-phenylendiacetic acid (H(2)PDA) under hydrothermal conditions produce isostructural lanthanide coordination polymers with the empirical formula [Ln(2)(PDA)(3)(H(2)O)] x 2H(2)O. The extended structure of [Ln(2)(PDA)(3)(H(2)O)] x 2H(2)O consists of Ln-COO triple helice... |
12617667 | Sensing and fixation of NO2/N2O4 by calix[4]arenes. | An approach toward visual detection and chemical utilization of NO(2)/N(2)O(4) is proposed, which employs simple calix[4]arenes. Exposure of tetra-O-alkylated calix[4]arenes 1 and 2, possessing either a cone or a 1,3-alternate conformation, to NO(2)/N(2)O(4), both in chloroform solution and in the solid state, results ... |
12617666 | Catalytic asymmetric aldol reactions in aqueous media using chiral bis-pyridino-18-crown-6-rare earth metal triflate complexes. | Catalytic asymmetric aldol reactions in aqueous media have been developed using Pr(OTf)(3) and chiral bis-pyridino-18-crown-6 1. In the asymmetric aldol reaction using rare earth metal triflates (RE(OTf)(3)) and 1, slight changes in the ionic diameters of the metal cations greatly affected the diastereo- and enantiosel... |
12617627 | Chloride transport across lipid bilayers and transmembrane potential induction by an oligophenoxyacetamide. | This contribution describes the discovery and properties of a synthetic, low-molecular weight compound that transports Cl- across bilayer membranes. Such compounds have potential as therapeutics for cystic fibrosis and cancer. The H+/Cl- co-transport activities of acyclic tetrabutylamides 1-6 were compared by using a p... |
12617626 | A non-covalent strategy for the assembly of supramolecular photocurrent-generating systems. | Three photocurrent-generating thin films were assembled on gold surfaces. SAM I was constructed from molecules consisting of an alkyl disulfide group linked covalently to a 12-residue helical peptide and terminated with an alanine residue containing a pyrene chromophore. SAM I served as a benchmark for multilayered fil... |
12617625 | Stepwise formation of heterobimetallic macrocycles synthesized via the weak-link approach. | A new dissymmetric bis-hemilabile ligand with strong binding phosphorus moieties, weak binding thioether moieties, and weaker binding ether moieties has been synthesized to construct heterobimetallic Rh(I)- and Pd(II)-containing macrocycles. The metals are placed in either the phosphorus/thioether or the phosphorus/eth... |
12618377 | Genotyping on a thermal gradient DNA chip. | Silicon-based chips with discrete, independently temperature-controlled islands have been developed for use in DNA microarray hybridization studies. Each island, containing a heater made of a diffusion layer and a temperature sensor based on a p-n junction, is created on a silicon dioxide/nitride surface by anisotropic... |
12617898 | Design, synthesis and binding affinity of 3'-fluoro analogues of Cl-IB-MECA as adenosine A3 receptor ligands. | Several 3'-fluoro analogues, 1a, 1b, and 1c of selective and potent adenosine A(3) receptor agonist, Cl-IB-MECA were synthesized from D-xylose via highly regioselective opening of lyxo-epoxides, 8a and 8b with fluoride anion. Compared to the high binding affinity of Cl-IB-MECA to the A(3) adenosine receptor, the corres... |
12615229 | Chiral and non chiral determination of Dopa by capillary electrophoresis. | The suitability of capillary electrophoresis for determining the enantiomeric purity of levodopa in a pharmaceutical formulation also containing benserazide was assessed. To this end, the pharmaceutical components were separated in a non-chiral medium that allowed the total amount of Dopa and that of benserazide to be ... |
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