id stringlengths 4 8 | title stringlengths 1 642 | abstract stringlengths 50 17.9k |
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15038700 | Formation of pseudorotaxane induced by electrochemical oxidation of ferrocene-containing axis molecule in the presence of crown ether. | The electrochemical oxidation of aminomethylferrocenes in the presence of dibenzo[24]crown-8 affords pseudorotaxanes containing a ferrocenyl group in its axis moiety. The formation of the pseudorotaxanes results from formation of secondary ammonium ions by rapid electron transfer from the N atom to the initially formed... |
15038695 | Enantioselective guest binding and dynamic resolution of cationic ruthenium complexes by a chiral metal-ligand assembly. | A supramolecular metal-ligand assembly encapsulates a variety of cationic half-sandwich ruthenium complexes. Due to the chirality of both host and guest, chiral recognition is observed with diastereomeric excesses up to 70%. The chiral cavity can be used to carry out a dynamic resolution of the rapidly equilibrating en... |
15038161 | Development of a microarray assay that measures hybridization stoichiometry in moles. | Microarray data is most useful when it can be compared with other genetic detection technologies. In this report, we designed a microarray assay format that transforms raw data into a defined scientific unit (i.e., moles) by measuring the amount of array feature present and the cDNA sequence hybridized. This study prof... |
15036110 | Stereochemistry of new nitrogen containing heterocyclic compounds.; X. Supramolecular structures and stereochemical versatility of polymeric complexes. | New polymeric complexes of Cu(II), Co(II), Ni(II) and UO2(II) with 5-(2(1)-carboxyphenylazo)-8-hydroxyquinoline (LH2) have been prepared and characterized on the basis of analytical, magnetic, 1H NMR, EPR and electronic spectral studies. Tentative structures for the polymeric complexes are proposed. The important infra... |
15036092 | Study of the inclusion processes of styrene and alpha-methyl-styrene in beta-cyclodextrin. | The inclusion complexes of styrene and alpha-methyl-styrene with beta-cyclodextrin (beta-CD) were investigated by using [1H] NMR titration in solution and X-ray diffraction (XRD) analysis, thermo-gravimetric analysis (TGA), elemental analysis (EA) in the solid state. The inclusion process has been studied by using PM3 ... |
15036595 | Zeta 14-3-3 protein favours the formation of human tau fibrillar polymers. | Tau protein can aggregate, in an aberrant way, in Alzheimer's disease and other tauopathies. The formation of those aggregates could take place in vitro by the addition of different compounds like polyanions or fatty acids and their derivates. Now, we found that a protein, zeta 14-3-3, facilitates the assembly of tau a... |
15037548 | PPAR gamma signaling exacerbates mammary gland tumor development. | Breast cancer cell lines that express the nuclear peroxisome proliferator-activated receptor gamma (PPAR gamma) can be prompted to undergo growth arrest and differentiation when treated with synthetic PPAR gamma ligands. To evaluate the therapeutic potential of increased PPAR gamma signaling in vivo, we generated trans... |
15036264 | Thrombospondin 1: a multifunctional protein implicated in the regulation of tumor growth. | Thrombospondins belong to a family of extracellular matrix (ECM) proteins widely found from embryonic to adult tissues. The modular structure of thrombospondins contains a series of peptide sequences implicated in a multiplicity of biological functions. Extracellular matrix undergoes important alterations under proteol... |
15032351 | Simulation of size exclusion chromatography for characterization of supramolecular complex: a theoretical study. | A simple chromatographic model was used to simulate the retention behavior of supramolecular complex in size exclusion chromatography (SEC). Two fundamental model complexes, directional complementary couple (AB) and self-complementary unit (AA), were employed to investigate the influence of various parameters in the SE... |
15032347 | Polymer-based adsorption medium prepared using a fragment imprinting technique for homologues of chlorinated bisphenol A produced in the environment. | A polymer-based adsorption medium having molecular recognition ability for homologues of chlorinated bisphenol A produced in environment was prepared using a fragment imprinting technique. 2,6-Dimethyl phenol was utilized as a pseudo-template molecule and the adsorption media prepared was evaluated by high performance ... |
15030216 | Structural characterization of imazalil/beta-cyclodextrin inclusion complex. | An equimolar inclusion complex between imazalil, a selected fungicide, and beta-cyclodextrin using an aqueous standard solution procedure has been obtained. The complex has been investigated in solution by (1)H and (13)C NMR techniques in combination with computational methods in order to establish a valuable analytica... |
15029526 | [Intranasal and buccal midazolam in the treatment of acute seizures]. | <h4>Aims</h4>There are several personal and social problems involved in the administration of rectal diazepam that make it unsuitable for use in public places and by non medical workers, in children and especially in teenagers and adults. Intranasal and oral midazolam could be an alternative to rectal diazepam. We revi... |
15029186 | Buckminsterfullerenes: a non-metal system for nitrogen fixation. | In all nitrogen-fixation processes known so far--including the industrial Haber-Bosch process, biological fixation by nitrogenase enzymes and previously described homogeneous synthetic systems--the direct transformation of the stable, inert dinitrogen molecule (N2) into ammonia (NH3) relies on the powerful redox proper... |
15028752 | Enantioselectivity of projection neurons innervating identified olfactory glomeruli. | Projection neurons (PNs) with arborizations in the sexually dimorphic "lateral large female glomerulus" (latLFG) in the antennal lobe (AL) of the moth Manduca sexta previously were shown to respond preferentially to antennal stimulation with (+/-)linalool, a volatile compound commonly emitted by plants. In the present ... |
15027855 | Countering cooperative effects in protease inhibitors using constrained beta-strand-mimicking templates in focused combinatorial libraries. | A major problem in de novo design of enzyme inhibitors is the unpredictability of the induced fit, with the shape of both ligand and enzyme changing cooperatively and unpredictably in response to subtle structural changes within a ligand. We have investigated the possibility of dampening the induced fit by using a cons... |
15027003 | The molecular basis of HIV capsid assembly--five years of progress. | The assembly of HIV is relatively poorly investigated when compared with the process of virus entry. Yet a detailed understanding of the mechanism of assembly is fundamental to our knowledge of the complete life cycle of this virus and also has the potential to inform the development of new antiviral strategies. The re... |
15025860 | Systemic and ocular pharmacokinetics of N-4-benzoylaminophenylsulfonylglycine (BAPSG), a novel aldose reductase inhibitor. | To better develop N-[4-(benzoylamino)phenylsulfonyl]glycine (BAPSG), a potent and selective aldose reductase inhibitor capable of delaying the progression of ocular diabetic complications, the objective of this study was to assess its pharmacokinetics. The plasma pharmacokinetics of BASPG was assessed in male Sprague-D... |
15027033 | Identification of differences in the specificity-determining residues of antibodies that recognize antigens of different size: implications for the rational design of antibody repertoires. | Studies of antibodies of known three-dimensional structure have revealed that insertion and deletion of amino acids at the hypervariable loops change the canonical structures, thus generating differences in the antigen-binding site topography. Such differences determine the size of the antigen with which the antibody i... |
15027032 | The role of flexibility and hydration on the sequence-specific DNA recognition by the Tn916 integrase protein: a molecular dynamics analysis. | The N-terminal domain of the Tn916 integrase protein (INT-DBD) is responsible for DNA binding in the process of strand cleavage and joining reactions required for transposition of the Tn916 conjugative transposon. Site-specific association is facilitated by numerous protein-DNA contacts from the face of a three-strande... |
15027031 | Kinetic and thermodynamic characterization of HIV-1 protease inhibitors. | Interaction kinetic and thermodynamic analyses provide information beyond that obtained in general inhibition studies, and may contribute to the design of improved inhibitors and increased understanding of molecular interactions. Thus, a biosensor-based method was used to characterize the interactions between HIV-1 pro... |
15027030 | Synthesis and receptor binding of IgG1 peptides derived from the IgG Fc region. | The IgG binding Fcgamma receptors (FcgammaRs) play a key role in defence against pathogens by linking humoral and cell-mediated immune responses. Impaired expression and/or function of FcgammaR may result in the development of pathological autoimmunity. Considering the functions of FcgammaRs, they are potential target ... |
15027029 | The substrate recognition mechanisms in chaperonins. | Chaperonins are a family of proteins devoted to assisting the folding of other proteins. They are large oligomers assembled into ring structures that enclose a cavity in which folding takes place. For this process to occur, the chaperonin must first recognize and interact with the unfolded polypeptide, then undergo a c... |
15025487 | Exceptionally stable, hollow tubular metal-organic architectures: synthesis, characterization, and solid-state transformation study. | An effective solvothermal procedure has been developed to synthesize the new three-dimensional metal-organic framework, [ZnF(AmTAZ)].solvents, using either 3-amino-1,2,4-triazole (AmTAZ) or 3-amino-1,2,4-triazole-5-carboxylic acid (AmTAZAc) and a choice of several Zn(II) salts as starting materials. The three-dimension... |
15025486 | Self-assembly of electron donor-acceptor dyads into ordered architectures in two and three dimensions: surface patterning and columnar "double cables". | We report the synthesis and characterization of covalent dyads and multiads of electron acceptors (A) and donors (D), with the purpose of exploiting their nanophase separation behavior toward (a) two-dimensional (2D) surface patterning with well-defined integrated arrays of dissimilar molecular electronic features and ... |
15025484 | Self-assembly of rod-coil molecules into molecular length-dependent organization. | A series of rod-coil molecules (n-x, where n represents the number of repeating units in a PPO coil and x the number of phenyl groups in a rod segment) with variation in the molecular length, but an identical rod to coil volume ratio was synthesized, and their self-assembling behavior was investigated by using DSC and ... |
15025483 | Ion-driven ATP pump by self-organized hybrid membrane materials. | We report new hybrid organic-inorganic materials, based on macrocyclic receptors 1-3 self-organized in tubular superstructures prepared by sol-gel process. Fourier transform infrared (FTIR) and NMR spectroscopic analyses demonstrate that the self-organization by hydrogen bonding of organogel superstructures of 2 and 3 ... |
15025475 | Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. | Using a recently described self-assembly process (Bayburt, T. H.; Grinkova, Y. V.; Sligar, S. G. Nano Letters 2002, 2, 853-856), we prepared soluble monodisperse discoidal lipid/protein particles with controlled size and composition, termed Nanodiscs, in which the fragment of dipalmitoylphosphatidylcholine (DPPC) bilay... |
15025467 | Asymmetric benzoin condensation catalyzed by chiral rotaxanes tethering a thiazolium salt moiety via the cooperation of the component: can rotaxane be an effective reaction field? | Although some reactions on rotaxanes have been reported, the characteristic features of the rotaxanes providing unique reaction fields have hardly been studied, especially as catalyst. In our continuous studies on interlocked molecules such as rotaxanes and catenanes, we have noticed the importance of such interlocked ... |
15025466 | Formation of hypervalent complexes of PhCCSiF3 with pyridine through intermolecular silicon...nitrogen interaction. | We report here the synthesis of trifluoro-phenylethynyl-silane (1) that forms with pyridine (Py), through intermolecular Si...N interaction, the pentacoordinate 1.Py complex and at low temperatures also the hexacoordinate 1.Py2 complex. 1H, 19F, 29Si, and 15N NMR spectra, as well as the first report for an intermolecul... |
15025442 | Convergent synthesis and photophysics of [60]fullerene/porphyrin-based rotaxanes. | A series of Sauvage-type rotaxanes containing [60]fullerene and tetraarylporphyrin moieties has been synthesized by a convergent route. Photoinduced energy-transfer and electron-transfer reactions in these rotaxanes yield long-lived change-separated states, in agreement with the large distance between the fullerene and... |
15025433 | An operational supramolecular nanovalve. | A functioning nanomachine in the form of a supramolecular nanovalve that opens and closes the orifices to molecular-sized pores and releases a small number of molecules on demand is reported. The nanovalve, which is used to open and close the nanocontainer, is a pseudorotaxane composed of two components-a long thread c... |
15024044 | PKCtheta signals activation versus tolerance in vivo. | Understanding the pathways that signal T cell tolerance versus activation is key to regulating immunity. Previous studies have linked CD28 and protein kinase C-theta (PKCtheta) as a potential signaling pathway that influences T cell activation. Therefore, we have compared the responses of T cells deficient for CD28 and... |
15023252 | Simultaneous determination of several antalgic drugs based on their interactions with beta-cyclodextrin by capillary zone electrophoresis. | The binding constants of beta-cyclodextrin (beta-CD) with antalgic drugs such as naproxen, ketoprofen, ibuprofen, acemetacin, and aspirin are determined by affinity capillary electrophoresis. Based on these interactions, a reliable method for the separation and simultaneous determinations of these compounds in the pres... |
15019020 | Investigating the potential of erythromycin and derivatives as chiral selector in capillary electrophoresis. | Macrocyclic antibiotics are present in an increasing number of chiral separation applications. In this study, erythromycin and some derivatives, belonging to the group of macrolide antibiotics, were investigated for their potential as chiral selector. Erythromycin A itself and five related substances namely erythromyci... |
15019074 | Prolongation of epidural bupivacaine effects with hyaluronic acid in rabbits. | To assess the prolongation of epidural bupivacaine by hyaluronic acid viscous formulations we designed a cross-over study in rabbits. Different doses of bupivacaine (3 or 6 mg) either as a solution (bupivacaine hydrochloride), or as viscous formulations with hyaluronic acid (bupivacaine base and bupivacaine hydrochlori... |
15019071 | Development of parenteral formulation for a novel angiogenesis inhibitor, CKD-732 through complexation with hydroxypropyl-beta-cyclodextrin. | The effect of hydroxypropyl-beta-cyclodextrin (HP-beta-CyD) on the aqueous solubility and chemical stability of O-(4-Dimethylaminoethoxycinnamoyl)fumagillol (CKD-732), a new angiogenesis inhibitor, was investigated with an aim of preparing a stable and effective parenteral formulation. The CKD-732/HP-beta-CyD inclusion... |
15018531 | Substituent effects on the site of electron transfer during the first reduction for gold(III) porphyrins. | Gold(III) porphyrins of the type (P-R)AuPF(6), where P = 5,10,15,20-tetrakis(3,5-di-tert-butylphenyl)porphyrin and R is equal to H (1), NO(2) (2), or NH(2) (3) which is substituted at one of the eight beta-pyrrolic positions of the macrocycle, were investigated as to their electrochemistry and spectroelectrochemistry i... |
15018523 | Characterization and purification of supramolecular metal complexes using gel-permeation chromatography. | Gel-permeation chromatography (GPC) has been used to analyze transition-metal-based squares, triangles, and related supramolecular complexes. Using rhenium-containing molecular squares of different sizes, a linear calibration curve has been established, which was used for confirming the relative sizes of other assembli... |
15018508 | Irreversible but noncovalent Ru(II)-pyridine bond: its use for the formation of [2]-catenanes. | The reaction between ligand 1, which consists of two terminal pyridines attached to a central 1,10-phenanthroline (phen), and the complex Ru(phen)2(CH3CN)2(PF6)2 has been studied. A new ruthenium containing metallamacrocycle has been obtained and fully characterized. Despite the relatively poor yield for the cyclizatio... |
15018506 | Octanuclear and nonanuclear supramolecular copper(II) complexes with linear "tritopic" ligands: structural and magnetic studies. | The structures and magnetic properties of self-assembled copper(II) clusters and grids with the "tritopic" ligands 2poap (a), Cl2poap (b), m2poap (c), Cl2pomp (d), and 2pomp (e) are described [ligands derived by reaction of 4-R-2,6-pyridinedicarboxylic hydrazide (R = H, Cl, MeO) with 2-pyridinemethylimidate (a-c, respe... |
15018496 | Structure of two azide salts of a copper(II) macrocycle and magnetic properties of Cu(14ane)Cu(N3)4. | Synthesis of azide complexes with the copper(II) macrocycle complex Cu(14ane)(2+) (where 14ane = 1,4,8,11-tetraazacyclothetradecane) has yielded two compounds. Cu(14ane)Cu(N(3))(4) contains micro(1,3)-azido bridged chains of Cu(14ane)(2+) cations and Cu(N(3))(4)(2)(-) anions. Magnetic studies reveal the presence of fer... |
15018983 | Solubility enhancement of celecoxib using beta-cyclodextrin inclusion complexes. | Celecoxib has very low water solubility. It forms a complex with beta-cyclodextrin (betaCD) both in aqueous and in solid state. It was observed that due to formation of the inclusion complex, the solubility and dissolution rate of celecoxib were enhanced. The formation of 1:1 complex with betaCD in solution was confirm... |
15018982 | Preparation and evaluation of drug-loaded gelatin nanoparticles for topical ophthalmic use. | Gelatin nanoparticles encapsulating pilocarpine HCl or hydrocortisone as model drugs were produced using a desolvation method. The influence of a number of preparation parameters on the particle properties was investigated. For the pilocarpine HCl-loaded spheres, an influence of the pH during particle preparation on th... |
15018975 | Association complexes between ovalbumin and cyclodextrins have no effect on the immunological properties of ovalbumin. | Delivery systems are designed to deliver necessary amounts of drugs without modifying their biological features. Cyclodextrins (CD) are potential candidates for such a role due to their ability to alter physical and chemical properties of guest molecules by the formation of inclusion/association complexes. They have al... |
15018971 | Development of acrylic-based copolymers for oral insulin delivery. | We developed nanospheres of crosslinked networks of methacrylic acid grafted with poly(ethylene glycol), and acrylic acid grafted with poly(ethylene glycol) nanospheres for use as oral insulin delivery devices. The copolymer nanospheres were synthesized via free-radical precipitation/dispersion. The average particle di... |
15018925 | Synthesis and duplex DNA recognition studies of oligonucleotides containing a ureido isoindolin-1-one homo-N-nucleoside. A comparison of host-guest and DNA recognition studies. | In an effort to construct non-natural bases to be used in triplex-based antigene DNA recognition strategies, a uriedo-isoindolin-1-one homo-N-nucleoside base was designed to bind the cytosine-guanine (CG) base pair. An organic soluble analogue of this base was shown to effectively complex CG (K(assoc)=740M(-1)) in chlo... |
15018906 | Gramicidin-based channel systems for the detection of protein-ligand interaction. | To detect protein-ligand interaction a gramicidin-based sensor was developed. Biotin was tagged to the C-terminus of gramicidin (Gram-bio 1). The biotin-moiety, which faces the electrolyte, gave little effect on single-channel conductance. Streptavidin added to the electrolyte was detected by Gram-bio 1 through the mon... |
15018905 | Engineering charge selectivity in model ion channels. | Most ion channel proteins exhibit some degree of charge selectivity, that is, an ability to conduct ions of one charge more efficiently than ions of the opposite charge. The structural origins of charge selectivity remain incompletely understood despite recent advances in the determination of cation-selective and anion... |
15018904 | Outer surface modification of synthetic multifunctional pores. | The characteristics of pores formed by p-octiphenyl beta-barrels with LWV triads at the outer surface are reported in comparison with the conventional rigid-rod beta-barrels with all-L outer surface. Maintained multifunctionality of tetrameric pores with external LWV triads (inversion of ion selectivity, molecular reco... |
15018900 | Design, synthesis, and characterization of peptide nanostructures having ion channel activity. | We report the synthesis and the functional studies of multiple crown alpha-helical peptides designed to form artificial ion channels. The approach combines the versatility of solid phase peptide synthesis, the conformational predictability of peptidic molecules, and the solution synthesis of crown ethers with engineera... |
15016364 | Crystal structure of the type III effector AvrB from Pseudomonas syringae. | AvrB is a Pseudomonas syringae type III effector protein that is translocated into host plant cells during attempted pathogenesis. Arabidopsis harboring the corresponding resistance protein RPM1 can detect AvrB and mount a rapid host defense response, thus avoiding active infection. In the plant cell, AvrB induces phos... |
15014524 | Supramolecular dendritic liquid quasicrystals. | A large number of synthetic and natural compounds self-organize into bulk phases exhibiting periodicities on the 10(-8)-10(-6) metre scale as a consequence of their molecular shape, degree of amphiphilic character and, often, the presence of additional non-covalent interactions. Such phases are found in lyotropic syste... |
15013091 | Neurokinin 1-receptors and sensory neuropeptides in tendon insertions at the medial and lateral epicondyles of the humerus. Studies on tennis elbow and medial epicondylalgia. | There is no information on the sensory innervation at the flexor muscle origin at the medial epicondyle of the humerus and it is not known if substance P receptors (Neurokinin 1-receptors, NK1-R) are present in tendon insertions in general. In the present investigation, we have studied the muscle origin in patients suf... |
15012148 | Synthetic creatinine receptor: imprinting of a Lewis acidic zinc(II)cyclen binding site to shape its molecular recognition selectivity. | Molecularly imprinted polymers (MIPs) from polymerizable Lewis acidic zinc(II)cyclen complexes and ethylene glycol dimethyl acrylate have been prepared. For the imprinting process the template molecule creatinine is reversibly coordinated to the zinc atom. The high strength of this interaction allows analyte binding to... |
15012144 | Nanosized molecular propellers by cyclodehydrogenation of polyphenylene dendrimers. | In a polymer analogous approach, large dendritic oligophenylenes containing benzene and tetraphenylmethane cores are transformed via oxidative cyclodehydrogenation to novel propeller-shaped molecules with large polycyclic aromatic hydrocarbon units as "blades". Structure analysis is performed by a combination of MALDI-... |
15012133 | Carotenoid and pheophytin on semiconductor surface: self-assembly and photoinduced electron transfer. | Self-assembling of a carotenoid and pheophytin a into a supramolecular system was observed on the surface of nanocrystalline TiO2, and the photoinduced electron-transfer reactions within the system were studied by means of femtosecond transient absorption and laser flash photolysis techniques. Excitation of the pheophy... |
15012131 | Enantioselective molecular recognition between beta-sheets. | This communication asks whether homochiral or heterochiral interaction is preferred between enantiomeric beta-sheets and finds that homochiral pairing is strongly preferred. Interactions between beta-sheets occur widely among proteins through pairing of the hydrogen-bonding edges. Although the hydrogen-bonding edges of... |
15012119 | Surface-to-surface bridges formed by reversibly assembled polymers. | Self-assembled polymers whose main chains are defined by reversible DNA base pairing form bridges between the tip of an atomic force microscope and substrate. The forces associated with the rupture of these assemblies are independent of polymer bridge length, and they resemble those expected for the isolated associatio... |
15012107 | Mineralomimetic sodalite- and muscovite-type coordination frameworks. Dynamic crystal-to-crystal interconversion processes sensitive to ion pair recognition. | A flexible sodalite-type metal organic framework [Cu(pyrimidine-2-olate)2]n (1R) is obtained in a self-assembly process involving Cu2+ ions and pyrimidine-2-olate ligands. 1R suffers a series of sequential and reversible structural changes upon solid-liquid sorption processes of metal nitrates. The 1C-to-1O transformat... |
15013987 | Prevalence of neutralizing antibodies to adenoviral serotypes 5 and 35 in the adult populations of The Gambia, South Africa, and the United States. | One of the major limitations of the use of adenoviruses as gene therapy vectors is the existence of preformed immunity in various populations. Recent studies have linked failure of adenoviral gene therapy trials to the presence of antiadenoviral neutralizing antibodies (NAb). Understanding the distribution and specific... |
15006452 | New evidence for the selective, long-lasting central effects of the brain-targeted estradiol, Estredox. | The present study examined the dose- and time-dependent central effects of an estradiol chemical delivery system cyclodextrin complex (E(2)-CDS-CD) on the reestablishment of copulatory behavior of castrated male and ovariectomized female rats with concomitant determination of the blood luteinizing hormone (LH) and E(2)... |
15007100 | Iron transport systems in Neisseria meningitidis. | Acquisition of iron and iron complexes has long been recognized as a major determinant in the pathogenesis of Neisseria meningitidis. In this review, high-affinity iron uptake systems, which allow meningococci to utilize the human host proteins transferrin, lactoferrin, hemoglobin, and haptoglobin-hemoglobin as sources... |
15007097 | Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism. | We present an analysis of over 1,100 of the approximately 10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa. Seven major areas of Neurospora genomics and biology are covered. First, the basic features of the genome, including the automated assembly, gene calls, and glo... |
15005624 | Quinones facilitate the self-assembly of the phosphorylated tubulin binding region of tau into fibrillar polymers. | The fragment of tau containing the first and third tubulin-binding motifs, involved in self-assembly of tau, was phosphorylated by protein kinase A (PKA). In the presence of hydroxynonenal (HNE) or in the presence of quinones such as juglone, 2,3-dimethoxy-5-methyl-1,4-benzoquinone (coenzyme Q(0) or DMM), or menadione,... |
15005607 | Conformational and molecular modeling studies of beta-cyclodextrin-heptagastrin and the third extracellular loop of the cholecystokinin 2 receptor. | The conformational features of a conjugate of the C-terminus of human gastrin (HG[11-17]), the shortest gastrin sequence retaining biological function, with beta-cyclodextrin ([Nle(15)]-HG[11-17]-betaCD) were determined by NMR spectroscopy in an aqueous solution of dodecylphosphocholine (DPC) micelles. The peptide-beta... |
15006538 | Agonist enhacement of macrocyclic lactone activity at a glutamate-gated chloride channel subunit from Haemonchus contortus. | The mode of action of ivermectin (IVM) in nematodes appears to be the opening of inhibitory ion channels, including the glutamate-gated chloride channel (GluCl). Recently, it has been shown that IVM binds with high affinity to a Haemonchus contortus GluCl subunit (HcGluCla) expressed in COS-7 cells, and this binding is... |
15006776 | 2-O-methylation of fucosyl residues of a rhizobial lipopolysaccharide is increased in response to host exudate and is eliminated in a symbiotically defective mutant. | When Rhizobium etli CE3 was grown in the presence of Phaseolus vulgaris seed extracts containing anthocyanins, its lipopolysaccharide (LPS) sugar composition was changed in two ways: greatly decreased content of what is normally the terminal residue of the LPS, di-O-methylfucose, and a doubling of the 2-O-methylation o... |
15006773 | Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification. | The chimeric gene encoding a C-terminally-truncated form of the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and two copies of the Fc-binding Z-domain was constructed, cloned, and heterologously expressed in Escherichia coli HMS174(DE3). The Z-domain is a synthetic analogue of the B-domain of protein A, capab... |
15003458 | The solution NMR structure of Antheraea polyphemus PBP provides new insight into pheromone recognition by pheromone-binding proteins. | Pheromone-binding proteins (PBPs) located in the antennae of male moth species play an important role in olfaction. They are carrier proteins, believed to transport volatile hydrophobic pheromone molecules across the aqueous sensillar lymph to the membrane-bound G protein-coupled olfactory receptor proteins. The roles ... |
15004846 | Polymeric alkenoxy amino acid surfactants: II. Chiral separations of beta-blockers with multiple stereogenic centers. | Two amino acid-based (leucine and isoleucine) alkenoxy micelle polymers were employed in this study for the separation of multichiral center-bearing beta-blockers, nadolol and labetalol. These polymers include polysodium N-undecenoxy carbonyl-L-leucinate (poly-L-SUCL) and polysodium N-undecenoxy carbonyl-L-isoleucinate... |
15004691 | Amyloid fibrils from the viewpoint of protein folding. | In amyloid related diseases, proteins form fibrillar aggregates with highly ordered beta-sheet structure regardless of their native conformations. Formation of such amyloid fibrils can be reproducible in vitro using isolated proteins/peptides, suggesting that amyloid fibril formation takes place as a result of protein ... |
15003016 | Acid- and salt-triggered multifunctional poly(propylene imine) dendrimer as a prospective drug delivery system. | A novel dendrimeric compound is designed with the objective of simultaneously addressing issues commonly encountered in drug delivery, i.e., stability in biological milieu as well as targeting. For this purpose, a multifunctional dendrimeric system derived from diaminobutane poly(propylene imine) dendrimers (DAB) is pr... |
15002999 | Biodegradable cellulose diacetate-graft-poly(L-lactide)s: thermal treatment effect on the development of supramolecular structures. | Thermal transition property of cellulose diacetate-graft-poly(L-lactide) (CDA-g-PLLA) varies depending seriously on the molar substitution (MS) of lactyl unit, as represented by a drastic Tg depression with increasing MS (0<MS< or =8) and a subsequent crystallization of the PLLA side chains (MS> or =14). To make clear ... |
15001355 | Structure of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy and single-particle image analysis. | The FliF ring is the base for self-assembly of the bacterial flagellum and the FliF/FliG ring complex is the core of the rotor of the flagellar motor. We report the structures of these two ring complexes obtained by electron cryomicroscopy and single-particle image analysis at 22A and 25A resolution, respectively. Dire... |
14998547 | Estimation of the proton affinity values of fifteen matrix-assisted laser desorption/ionization matrices under electrospray ionization conditions using the kinetic method. | The kinetic method, which is known to be simple, fast and precise, is used for the measurement of proton affinity values of fifteen selected matrix-assisted laser desorption/ionization matrices under electrospray ionization conditions. The stabilization of [M - H](-)/[M + Matrix - H](-) ions for beta-cyclodextrin in ne... |
14998537 | Remarkable amine-TFA self assembly. | Supramolecular assemblies that are formed between amines and trifluoroacetic acid were studied using electrospray ionization mass spectrometry. Distinctive association behavior of primary, secondary, and tertiary amines with trifluoroacetic acid upon identical experimental conditions is observed and indicates that ster... |
14996807 | Interaction of the crystalline bacterial cell surface layer protein SbsB and the secondary cell wall polymer of Geobacillus stearothermophilus PV72 assessed by real-time surface plasmon resonance biosensor technology. | The interaction between S-layer protein SbsB and the secondary cell wall polymer (SCWP) of Geobacillus stearothermophilus PV72/p2 was investigated by real-time surface plasmon resonance biosensor technology. The SCWP is an acidic polysaccharide that contains N-acetylglucosamine, N-acetylmannosamine, and pyruvic acid. F... |
14995211 | Kinetics and thermodynamics of hexameric capsule formation. | Resorcinarene 1b forms a hexameric assembly in water-saturated CDCl(3) that encapsulates one tetraalkylammonium salt (2(+)Br(-)). The remaining space is occupied by coencapsulated solvent molecules. A maximum of three and minimum of one CHCl(3) molecule were found inside of capsules with tetrapropyl- and tetraheptylamm... |
14995204 | Kinetically stable complexes in water: the role of hydration and hydrophobicity. | We describe here the synthesis and characterization of a molecular receptor that forms kinetically and thermodynamically stable host-guest complexes in water. This cavitand-based host is composed of a preorganized aromatic pocket whose rim is decorated with four negatively charged carboxylate groups. (1)H NMR and isoth... |
14995200 | Multielectron chemistry of zinc porphyrinogen: a ligand-based platform for two-electron mixed valency. | The synthesis, electronic structure, and oxidation-reduction chemistry of a homologous series of Zn(II) porphyrinogens are presented. The fully reduced member of the series, [LZn](2-), was prepared in two steps from pyrrole and acetone. The compound undergoes consecutive two-electron, ligand-based, oxidations at +0.21 ... |
14997213 | Selective disruption of the E-cadherin-catenin system by an algal toxin. | Yessotoxins (YTXs) are algal toxins that can be accumulated in edible molluscs. YTX treatment of MCF-7 breast cancer cells causes the accumulation of a 100 kDa fragment of E-cadherin, which we have named ECRA(100). A relative decrease in the concentrations of intact E-cadherin did not accompany the accumulation of ECRA... |
14993639 | Influence of flanking sequences on presentation efficiency of a CD8+ cytotoxic T-cell epitope delivered by parvovirus-like particles. | We have previously developed an antigen-delivery system based on hybrid recombinant porcine parvovirus-like particles (PPV-VLPs) formed by the self-assembly of the VP2 protein of PPV carrying a foreign epitope at its N terminus. In this study, different constructs were made containing a CD8(+) T-cell epitope of chicken... |
14993249 | Mapping of a functional recombination motif that defines isotype specificity for mu-->gamma3 switch recombination implicates NF-kappaB p50 as the isotype-specific switching factor. | Ig class switch recombination (CSR) requires expression of activation-induced deaminase (AID) and production of germline transcripts to target S regions for recombination. However, the mechanism of CSR remains unclear. Here we show that an extrachromosomal S plasmid assay is AID dependent and that a single consensus re... |
14993753 | Separation of stereoisomers of some terpene derivatives by capillary gas chromatography-mass spectrometry and high-performance liquid chromatography using beta-cyclodextrin derivative columns. | Gas chromatographic separations of the stereoisomers of menthol derivatives, important intermediates in the synthesis of physiologically active natural products, were carried out on several substituted beta-cyclodextrin (CD) columns, including per-O-methyl-beta-cyclodextrin (PME-beta-CD), heptakis(2,3-di-O-acetyl-6-ter... |
14990263 | A new form of bacterial movement, dragging of multicellular aggregate structures over solid surfaces, is powered by macrofiber supercoiling. | Growing Bacillus subtilis macrofibers use twist and supercoiling to: power their own self-assembly, join fibers together into multiclonal aggregates, move themselves over solid surfaces, and to drag other structures (cargo) over solid surfaces. The dragging of multiclonal aggregates attached to the ends of growing macr... |
14990736 | The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5. | The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to its core epitope on gp41, ELDKWAS, revealed that the peptide interacts with residues at the base of the unusually long (... |
14990734 | The amino-terminal region of major capsid protein P3 is essential for self-assembly of single-shelled core-like particles of Rice dwarf virus. | The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one of which is inserted by the amino-terminal region of another P3 protein. The P3 proteins with serial amino-terminal deletions, expressed in a baculovirus system, formed particles with gradually decreasing stability. The capacity for self-assembly... |
14990731 | Crystal structures of a multidrug-resistant human immunodeficiency virus type 1 protease reveal an expanded active-site cavity. | The goal of this study was to use X-ray crystallography to investigate the structural basis of resistance to human immunodeficiency virus type 1 (HIV-1) protease inhibitors. We overexpressed, purified, and crystallized a multidrug-resistant (MDR) HIV-1 protease enzyme derived from a patient failing on several protease ... |
14990728 | Cholesterol is required for endocytosis and endosomal escape of adenovirus type 2. | The species C adenovirus type 2 (Ad2) and Ad5 bind the coxsackievirus B Ad receptor and alphav integrin coreceptors and enter epithelial cells by clathrin-mediated endocytosis. This pathway is rapid and efficient. It leads to cell activation and the cholesterol-dependent formation of macropinosomes. Macropinosomes are ... |
14990720 | Treatment with anti-LFA-1 delays the CD8+ cytotoxic-T-lymphocyte response and viral clearance in mice with primary respiratory syncytial virus infection. | Cytotoxic T lymphocytes (CTLs) play an important role in the immune response against respiratory syncytial virus (RSV) infection. The cell surface molecule lymphocyte function-associated antigen 1 (LFA-1) is an important contributor to CTL activation, CTL-mediated direct cell lysis, and lymphocyte migration. In an atte... |
14990688 | Requirements for CEACAMs and cholesterol during murine coronavirus cell entry. | Previous reports have documented that cholesterol supplementations increase cytopathic effects in tissue culture and also intensify in vivo pathogenicities during infection by the enveloped coronavirus murine hepatitis virus (MHV). To move toward a mechanistic understanding of these phenomena, we used growth media enri... |
14991059 | New insights into atopic dermatitis. | Atopic dermatitis is a chronic inflammatory skin disease associated with cutaneous hyperreactivity to environmental triggers and is often the first step in the atopic march that results in asthma and allergic rhinitis. The clinical phenotype that characterizes atopic dermatitis is the product of interactions between su... |
14989661 | Stereochemistry in functionalized macrocycle complexes: control of hydroxyl substituent orientation. | Use of a hydroxyl-functionalized open chain tetramine in a template reaction based on its Cu(II) complex leads, after reduction, to a new tetraaza macrocycle with both amino and hydroxyl substituents. The macrocycle is formed predominantly as its trans (anti) isomer, though the cis form is detectable and both have been... |
14989660 | Functionalized macrocycles from functionalized tetra-amines: pendent-arm macrocycles derived from dichloropivalic acid. | Reaction between ethane-1,2-diamine and 3,3'-dichloropivalic acid results in different, isomeric tetra-amine derivatives, one a tetraamino carboxylic acid and the other a carboxamidotriamino alcohol, depending upon reaction conditions. Intended conversion of the Cu(II) complex of the former to a cyclam-like macrocycle ... |
14991662 | Inclusion complex of fenbufen with beta-cyclodextrin. | An inclusion complex of fenbufen with beta-cyclodextrin (beta-CD) in aqueous solution was characterized by (1)H-NMR spectroscopy. The [1:1] stoichiometry was determined and a stability constant of several 1000s (M(-1)) was calculated. The geometry of the inclusion complex was established based on MM(+) molecular mechan... |
14990511 | Hydration dynamics near a model protein surface. | The evolution of water dynamics from dilute to very high concentration solutions of a prototypical hydrophobic amino acid with its polar backbone, N-acetyl-leucine-methylamide (NALMA), is studied by quasi-elastic neutron scattering (QENS) and molecular dynamics (MD) simulation for both the completely deuterated and com... |
14990499 | Mapping oxygen accessibility to ribonuclease a using high-resolution NMR relaxation spectroscopy. | Paramagnetic contributions to nuclear magnetic spin-lattice relaxation rate constant induced by freely diffusing molecular oxygen measured at hundreds of different protein proton sites provide a direct means for characterizing the exploration of the protein by oxygen. This report focuses on regions of ribonuclease A wh... |
14990489 | Effects of HU binding on the equilibrium cyclization of mismatched, curved, and normal DNA. | The effects of HU, the histone-like protein from Escherichia coli, on the equilibrium cyclization of duplex DNAs have been observed as a function of protein concentration and DNA sequence. The results indicate that the presence of HU significantly enhances the extent of cyclization and increases the melting temperature... |
14990486 | Molecular dynamics simulation of a palmitoyl-oleoyl phosphatidylserine bilayer with Na+ counterions and NaCl. | Two 40 ns molecular dynamics simulations of a palmitoyl-oleoyl phosphatidylserine (POPS) lipid bilayer in the liquid crystalline phase with Na(+) counterions and NaCl were carried out to investigate the structure of the negatively charged lipid bilayer and the effect of salt (NaCl) on the lipid bilayer structure. Na(+)... |
14990481 | Scrutiny of the failure of lipid membranes as a function of headgroups, chain length, and lamellarity measured by scanning force microscopy. | A fast, quantitative, and unambiguous screening of material properties of biomembranes using scanning force microscopy in pulsed force mode on lipid membranes is presented. The spatially resolved study of breakthrough force, breakthrough distance, adhesion, stiffness, and topography of lipid membranes as determined sim... |
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