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15039342 | Detergent-resistant membrane microdomains facilitate Ib oligomer formation and biological activity of Clostridium perfringens iota-toxin. | Clostridium perfringens iota-toxin consists of two separate proteins identified as a cell binding protein, iota b (Ib), which forms high-molecular-weight complexes on cells generating Na(+)/K(+)-permeable pores through which iota a (Ia), an ADP-ribosyltransferase, presumably enters the cytosol. Identity of the cell rec... |
15034047 | Depletion of cellular cholesterol and lipid rafts increases shedding of CD30. | CD30, a lymphoid activation marker, is shed into the cell environment after endoproteolytic cleavage of its ectodomain. Soluble (s)CD30 is able to suppress the Th1-type immune response. Because high serum levels of sCD30 and cholesterol-lowering drugs seem to be beneficial in some Th1-type autoimmune diseases, we focus... |
15030893 | A selective and sensitive assay of berberine using total internal reflected resonance light scattering technique with fluorescein at the water/1,2-dichloroethane interface. | An assay of berberine (BE) was developed with good selectivity and sensitivity based on the total internal reflected resonance light scattering (TIR-RLS) signals from water/1,2-dichloroethane (H(2)O/DCE) interface. Under optimal conditions, amphiphilic complex formed by BE and fluorescein (Flu) was adsorbed to H(2)O/DC... |
15030882 | Physicochemical characterization and dissolution properties of meloxicam-cyclodextrin binary systems. | The objective of the work is physicochemical characterization of meloxicam (ME)-cyclodextrin (CD) binary systems both in solution and solid states and to improve the dissolution properties of meloxicam via complexation with alpha-, beta- and gamma-cyclodextrins. Detection of inclusion complexation was done in solution ... |
15030879 | Spectrophotometric and electrochemical study of the inclusion complex between beta-cyclodextrin and furnidipine. | Inclusion complexation between furnidipine (2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylic acid 3-tetrahydrofurfuryl 5-methyl diester), a calcium-channel antagonist, and beta-cyclodextrin (beta-CyD) was studied in aqueous solution by using both spectrophotometric and electrochemical measurements. T... |
15026071 | Cyclodextrin trimers as receptors for arranging ester and catalyst at optimized location to achieve enhancement of hydrolytic activity. | Two novel trimer receptor molecules (AC and AD trimers) consisting of two alpha-cyclodextrins (alpha-CDs) and one beta-cyclodextrin (beta-CD) have been designed and synthesized in order to make an optimum arrangement between a substrate and a catalyst. In the reaction systems that use trimer receptors as host molecules... |
15026053 | Complestatin synthetic studies; the effect of the amino acid configuration on peptide backbone conformation in the common western BCD macrocycle. | The synthesis and structural analysis, involving X-ray crystallographic, nuclear magnetic resonance, and computational studies of four diastereomers of the common western BCD diarylether macrocycle of the complestatins, a family of HIV entry inhibitors, has been achieved exploiting a ruthenium-promoted intramolecular S... |
15026036 | Discovery and SAR of trisubstituted thiazolidinones as CCR4 antagonists. | Substituted thiazolidinones were identified as CCR4 antagonists from high throughput screening. Subsequent lead optimization efforts resulted in defined structure-activity relationships and the identification of potent antagonists (compounds 90 and 91) that inhibited the chemotaxis of Th2 T-cells in vitro. |
15024090 | Chromatin-mediated restriction of nuclear factor 1/CTF binding in a repressed and hormone-activated promoter in vivo. | Mouse mammary tumor virus (MMTV) promoter-driven transcription is induced by glucocorticoid hormone via binding of the glucocorticoid receptor (GR). The MMTV promoter also harbors a binding site for nuclear factor 1 (NF1). NF1 and GR were expressed in Xenopus oocytes; this revealed GR-NF1 cooperativity both in terms of... |
15024059 | Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes. | Exchange of the glucocorticoid receptor (GR) at promoter target sites provides the only known system in which transcription factor cycling at a promoter is fast, occurring on a time scale of seconds. The mechanism and function of this rapid exchange are unknown. We provide evidence that proteasome activity is required ... |
15024750 | Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse. | The supramolecular anchoring/signaling complex at the postsynaptic density of glutamatergic synapses has been proposed to play a key role in regulating synaptic function and plasticity. One class of proteins present in the complex is the SAP90/PSD-95-associated protein family (SAPAPs). The SAPAPs, identified by their d... |
15020236 | Streptomyces lividans potassium channel KcsA is regulated by the potassium electrochemical gradient. | It is currently held that the Streptomyces lividans potassium channel, KcsA, requires an intracellular pH<5 to exhibit activity in planar lipid bilayers. Here, we show that KcsA functions well at normal physiological pH in the presence of a potassium electrochemical gradient. Single-channel conductance and open probabi... |
15016867 | High-resolution phylogenetic analysis of hepatitis C virus adaptation and its relationship to disease progression. | Hepatitis C virus (HCV) persists in the majority of those infected despite host immune responses. Evidence has accrued that selectively fixed mutations in the envelope genes (E1 and E2) are associated with viral persistence, particularly those that occur within the first hypervariable region of E2 (HVR1). However, the ... |
15016849 | N-linked glycosylation of the V3 loop and the immunologically silent face of gp120 protects human immunodeficiency virus type 1 SF162 from neutralization by anti-gp120 and anti-gp41 antibodies. | We examined how asparagine-linked glycans within and adjacent to the V3 loop (C2 and C3 regions) and within the immunologically silent face (V4, C4, and V5 regions) of the human immunodeficiency virus (HIV) SF612 envelope affect the viral phenotype. Five of seven potential glycosylation sites are utilized when the viru... |
15011256 | Development and evaluation of a generic evolutionary method for protein-ligand docking. | We have developed a generic evolutionary method with an empirical scoring function for the protein-ligand docking, which is a problem of paramount importance in structure-based drug design. This approach, referred to as the GEMDOCK (Generic Evolutionary Method for molecular DOCKing), combines both continuous and discre... |
15010130 | Haemolytic properties of some water-soluble para-sulphonato-calix-[n]-arenes. | In this paper, we describe the haemolytic effect of parent para-sulphonato-calix-[n]-arenes and their derivatives bearing one pendant group at the lower rim of calix-arene towards human erythrocytes. A maximum of 30% of haemolysis has been observed for para-sulphonato-calix-[8]-arene for a concentration of 200 mM repre... |
14999743 | Cytotoxicity evaluation of enzyme inhibitors and absorption enhancers in Caco-2 cells for oral delivery of salmon calcitonin. | The usefulness of enzyme inhibitors and absorption enhancers with least mucosal cell cytotoxicity was evaluated on Caco-2 cell monolayers. The temporal cytotoxicity of several protease inhibitors at 500 microg/mL (e.g., turkey and chicken ovomucoids, aprotinin, and Protease Inhibitor Cocktail) and absorption enhancers ... |
14999719 | Biopharmaceutics of beta-cyclodextrin derivative-based formulations of acitretin in Sprague-Dawley rats. | Acitretin, an active metabolite of etretinate, is as effective as etretinate in the treatment of psoriasis. Recently, we developed some water-soluble formulations of acitretin with 2-hydroxypropyl-beta-cyclodextrin (HPBCD)/randomly substituted methyl-beta-cyclodextrin (RMBCD). In this study, the biopharmaceutic propert... |
15252641 | Electron paramagnetic resonance studies on copper(II)-cyclodextrin systems. | Three copper(II) complexes containing beta-cyclodextrin (betaCD) derivatives as ligands [mono-6-methylamino-6-deoxy-betaCD dithiocarbamate (CDTC), mono-6-histamino-6-deoxy-betaCD (CDHM) and mono-6-Nalpha-arginyl-6-deoxy-betaCD (CDARG)] have been studied by electron paramagnetic resonance. The spectra have been recorded... |
15056758 | The HP-1 maquette: from an apoprotein structure to a structured hemoprotein designed to promote redox-coupled proton exchange. | Synthetic heme-binding four-alpha-helix bundles show promise as working model systems, maquettes, for understanding heme cofactor-protein assembly and function in oxidoreductases. Despite successful inclusion of several key functional elements of natural proteins into a family of heme protein maquettes, the lack of 3D ... |
15071237 | Hydrogen-bonded supramolecular motifs in 4,4'-bipyridinium 8-hydroxy-7-iodoquinoline-5-sulfonate dihydrate. | In the title compound, C(10)H(9)N(2)(+).C(9)H(5)INO(4)S(-).2H(2)O, the 4,4'-bipyridine molecule is protonated at one of the pyridine N atoms. These moieties self-assemble into a supramolecular chain along the a axis through N-H.N hydrogen bonds. The quinolinol OH group acts as a donor with respect to a sulfonate O atom... |
15071235 | 1,14-dioxa-5,10-diaza-2,3:12,13-dibenzocyclooctadeca-2,12-diene monohydrate. | The title compound, C(22)H(30)N(2)O(2).H(2)O, is an 18-membered diaza-crown ether ligand containing two ether O and two aza N atoms. In the macrocyclic ring, the mean N.O distance is 4.526 (4) A. The macrocyclic inner-hole size, estimated as twice the mean distance of the donor atoms from their centroid, is approximate... |
15071234 | 3,3,10,10-tetramethyl-1,2-dithia-5,8-diazacyclodeca-4,8-diene. | The title compound, C(10)H(18)N(2)S(2), acts as an important precursor for the synthesis of the pharmaceutically important diaminedithiol ligand system. The molecule has a local twofold axis and the arrangement of the S(2)N(2) donor atoms in the macrocycle is anticlinal. |
15054106 | Polarity and lipid raft association of the components of the ciliary neurotrophic factor receptor complex in Madin-Darby canine kidney cells. | Ciliary neurotrophic factor (CNTF) signals via a tripartite receptor complex consisting of the glycosyl-phosphatidylinositol (GPI)-anchored CNTF receptor (CNTF-R), the leukaemia inhibitory factor receptor (LIF-R) and the interleukin-6 (IL-6) signal transducer gp130. We have recently reported that gp130 is endogenously ... |
15116180 | Solid-state caging of 1,10-phenanthroline pi-pi stacked dimers by calix[4]arene dihydroxyphosphonic acid. | The calix[4]arene dihydroxyphosphonic acid-1,10-phenanthroline complex shows caging of the guest molecules as a pi-pi stacked dimer in a cavity formed by intermolecular hydrogen bonds and aromatic walls formed by the calixarene. |
15054139 | A single amino acid substitution in human APOBEC3G antiretroviral enzyme confers resistance to HIV-1 virion infectivity factor-induced depletion. | HIV-1 and other retroviruses occasionally undergo hypermutation, characterized by a high rate of G-to-A substitution. Recently, the human apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G (APOBEC3G), first identified as CEM15, was shown to be packaged into retroviral virions and to deaminate deoxycyt... |
15198540 | Triamterene-beta-cyclodextrin systems: preparation, characterization and in vivo evaluation. | The purpose of this research was to improve the solubility and therefore dissolution and bioavailability of triamterene, a poorly water soluble diuretic, by complexation with beta-cyclodextrin. Triamterene has been reported to show low bioavailability after oral administration, with wide intersubject variation. This st... |
15051811 | A third base pair for the polymerase chain reaction: inserting isoC and isoG. | Two additional bases (isoguanosine and isocytosine), generating a third base pair, have been implemented in PCR. Enzyme fidelity for the third base pair is demonstrated using molecular thermodynamic melting, chemical cleavage and molecular beacons. When amplifying as few as 15 targets containing multiple non-natural ba... |
15048107 | The tetrameric L27 domain complex as an organization platform for supramolecular assemblies. | L27 domain, initially identified in the Caenorhabditis elegans Lin-2 and Lin-7 proteins, is a protein interaction module that exists in a large family of scaffold proteins. The domain can function as an organization center of large protein assemblies required for establishment and maintenance of cell polarity. We have ... |
15252635 | Aminoacid N-substituted 1,4,7-triazacyclononane and 1,4,7,10-tetraazacyclododecane Zn2+, Cd2+ and Cu2+ complexes. A preparative, potentiometric titration and NMR spectroscopic study. | The pK(a)s and Zn2+, Cd2+ and Cu2+ complexation constants (K) for 1,4,7-tris[(2''S)-acetamido-2''-(methyl-3''-phenylpropionate)]-1,4,7-triazacyclononane, 1, 1,4,7-tris[(2''S)-acetamido-2''-(1''-carboxy-3''-phenylpropane)]-1,4,7-triazacyclononane, H(3)2, 1,4,7-tris[(2''S)-acetamido-2''-(methyl-3''-(1H-3-indolyl)propiona... |
15252630 | Cation sensors containing a (bpy)Re(CO)3 group linked to an azacrown ether via an alkenyl or alkynyl spacer: synthesis, characterisation, and complexation with metal cations in solution. | Two [(bpy)Re(CO)3L]+ complexes (bpy = 2,2'-bipyridine), where L contains an aza-15-crown-5 ether which is linked to Re via an alkenyl- or alkynyl-pyridine spacer, have been synthesised along with model complexes. Solutions of the complexes in acetonitrile have been studied by UV-Vis absorption spectroscopy, and by 1D a... |
15047759 | Emergence of new regulatory mechanisms in the Benson-Calvin pathway via protein-protein interactions: a glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex. | Protein-protein interactions are involved in many metabolic pathways. This review will focus on the role of such associations in CO2 assimilation (Benson-Calvin cycle) and especially on the involvement of a GAPDH/CP12/PRK complex which has been identified in many photosynthetic organisms and may have an important role ... |
15044453 | Supramolecular organization of cytochrome c oxidase- and alternative oxidase-dependent respiratory chains in the filamentous fungus Podospora anserina. | To elucidate the molecular basis of the link between respiration and longevity, we have studied the organization of the respiratory chain of a wild-type strain and of two long-lived mutants of the filamentous fungus Podospora anserina. This established aging model is able to respire by either the standard or the altern... |
15044703 | Complex molecular architecture of beet yellows virus particles. | Closteroviruses possess exceptionally long filamentous virus particles that mediate protection and active transport of the genomic RNA within infected plants. These virions are composed of a long "body" and short "tail" whose principal components are the major and minor capsid proteins, respectively. Here we use bioche... |
15040800 | A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol. | <h4>Background</h4>Mutations in either of two genes comprising the STSL locus, ATP-binding cassette (ABC)-transporters ABCG5 (encoding sterolin-1) and ABCG8 (encoding sterolin-2), result in sitosterolemia, a rare autosomal recessive disorder of sterol trafficking characterized by increased plasma plant sterol levels. B... |
15252625 | Unexpected formation of a copper(II) 12-metallacrown-4 with (S)-glutamic-gamma-hydroxamic acid: a thermodynamic and spectroscopic study in aqueous solution. | The equilibria of copper(II) with (S)-glutamic-gamma-hydroxamic acid (H2L) were investigated in aqueous solution by different techniques: glass electrode potentiometry; calorimetry; VIS and CD spectrophotometry; and ES-MS. An unexpected pentacopper(II) 12-metallacrown-4 [Cu5L4H(-4)](2-) was detected, analogous to those... |
15044691 | Apoptosis or growth arrest: Modulation of tumor suppressor p53's specificity by bacterial redox protein azurin. | The tumor suppressor protein p53 is known to induce either apoptosis or growth arrest depending on cellular background. We have previously reported that a bacterial redox protein azurin induces apoptosis in J774 cell line-derived macrophages whereas a site-directed mutant M44KM64E azurin shows very little cytotoxicity ... |
15069528 | Supramolecular assembly of a quaterthiophene surfactant. | A newly synthesized oligothiophene surfactant self-assembles into unilamellar vesicles in aqueous solution and exhibits semiconductive behavior as a cast film. |
15039455 | Cell type-specific recruitment of Drosophila Lin-7 to distinct MAGUK-based protein complexes defines novel roles for Sdt and Dlg-S97. | Stardust (Sdt) and Discs-Large (Dlg) are membrane-associated guanylate kinases (MAGUKs) involved in the organization of supramolecular protein complexes at distinct epithelial membrane compartments in Drosophila. Loss of either Sdt or Dlg affects epithelial development with severe effects on apico-basal polarity. Moreo... |
15044473 | Reversible pore block of connexin channels by cyclodextrins. | Cyclodextrins (CDs), a series of hollow cyclic glucosaccharides, can reversibly block molecular permeation through channels formed by connexin-32 and/or connexin-26 reconstituted into liposomes. The character of the block changes as a function of the size of the CD relative to the connexin pore diameter, suggesting tha... |
15103406 | Cyclam complexes and their applications in medicine. | Cyclams are 14-membered tetraamine macrocycles which bind strongly to a wide range of metal ions. Medical interest has centred on clinical trials of a bicyclam for the treatment of AIDS and for stem cell mobilization, and on adducts with Tc and Cu radionuclides for diagnosis and therapy. Other potential applications pa... |
15035675 | Library on a slide for bacterial comparative genomics. | <h4>Background</h4>We describe a novel application of microarray technology for comparative genomics of bacteria in which libraries of entire genomes rather than the sequence of a single genome or sets of genes are arrayed on the slide and then probed for the presence or absence of specific genes and/or gene alleles.<h... |
15037754 | The unique insert in myosin VI is a structural calcium-calmodulin binding site. | Myosin VI contains an inserted sequence that is unique among myosin superfamily members and has been suggested to be a determinant of the reverse directionality and unusual motility of the motor. It is thought that each head of a two-headed myosin VI molecule binds one calmodulin (CaM) by means of a single "IQ motif". ... |
15037598 | Nerve-independent formation of a topologically complex postsynaptic apparatus. | As the mammalian neuromuscular junction matures, its acetylcholine receptor (AChR)-rich postsynaptic apparatus is transformed from an oval plaque into a pretzel-shaped array of branches that precisely mirrors the branching pattern of the motor nerve terminal. Although the nerve has been believed to direct postsynaptic ... |
15071231 | Molecular conformation and supramolecular aggregation in two fused pyrazoles: pi-stacked R(2)(2)(6) dimers in 2,8,8-trimethyl-6,7,8,9-tetrahydropyrazolo[2,3-a]quinazolin-6-one, and sheets of alternating R(2(2)12) and R(6)(6)(48) rings in 3-tert-butyl-4',4'-dimethyl-1-phenyl-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-b]pyridine... | In 2,8,8-trimethyl-6,7,8,9-tetrahydropyrazolo[2,3-a]quinazolin-6-one, C(13)H(15)N(3)O, (I), the heterobicyclic system is planar and exhibits peripheral ten pi-electron delocalization. In 3-tert-butyl-4',4'-dimethyl-1-phenyl-4,5,6,7-tetrahydro-1H- pyrazolo[3,4-b]pyridine-5-spiro-1'-cyclohexane-2',6'-dione, C(23)H(25)N(3... |
15034168 | Recognition of single-stranded RNA viruses by Toll-like receptor 7. | Viral infection of mammalian host results in the activation of innate immune responses. Toll-like receptors (TLRs) have been shown to mediate the recognition of many types of pathogens, including viruses. The genomes of viruses possess unique characteristics that are not found in mammalian genomes, such as high CpG con... |
15070724 | Molecular clamp mechanism of substrate binding by hydrophobic coiled-coil residues of the archaeal chaperone prefoldin. | Prefoldin (PFD) is a jellyfish-shaped molecular chaperone that has been proposed to play a general role in de novo protein folding in archaea and is known to assist the biogenesis of actins, tubulins, and potentially other proteins in eukaryotes. Using point mutants, chimeras, and intradomain swap variants, we show tha... |
15069483 | A chiral molecular bowl containing three ferrocenes: synthesis and its efficiency in an optical resolution of 1,1'-bi-2-naphthol. | Condensation reaction of 1,1'-ferrocenedicarboxaldehyde with (1R,2R)-1,2-diaminocyclohexane affords a novel bowl-shaped macrocycle with a chiral concave cavity which exhibits a remarkable ability as a host material for the enantioselective enclathration of 1,1'-bi-2-naphthol. |
15034146 | Silencer elements as possible inhibitors of pseudoexon splicing. | Human pre-mRNAs contain a definite number of exons and several pseudoexons which are located within intronic regions. We applied a computational approach to address the question of how pseudoexons are neglected in favor of exons and to possibly identify sequence elements preventing pseudoexon splicing. A search for pos... |
15034722 | Structure-activity relationships of three differently substituted 2,7,12,17-tetrakis-(beta-methoxyethyl) porphycene derivatives in vitro. | The subcellular localization, efficacy and photooxidative mechanism of three new photosensitizing porphycenes (HexoTMPn, PeloTMPn, CpoTMPn) for photodynamic therapy with different substituents at position 9 of the tetrapyrrole macrocycle were investigated in vitro using different human skin-derived cell lines (HaCaT, S... |
15064797 | Cyclodextrin effect on solvolysis of substituted benzoyl chlorides. | A kinetic study was carried out on the solvolysis of substituted benzoyl chlorides in the presence of alpha-, beta- and gamma-CD. Combination of the substituent dependent mechanism for solvolysis of benzoyl chlorides and the complexation ability of the cyclodextrin yields the following experimental behavior: (i) cataly... |
15029242 | Structural basis for host recognition by the Haemophilus influenzae Hia autotransporter. | Haemophilus influenzae is an important human pathogen that initiates infection by colonizing the upper respiratory tract. The H. influenzae Hia autotransporter is an adhesive protein that promotes adherence to respiratory epithelial cells. Hia adhesive activity resides in two homologous binding domains, called HiaBD1 a... |
15017094 | Crystal structure of the supramolecular linear polymer formed by the self-assembly of mono-6-deoxy-6-adamantylamide-beta-cyclodextrin. | Mono-6-deoxy-6-adamantylamide-beta-cyclodextrin-dimethylformamide-15H2O, C53H85NO35.C3H7NO.15H2O, crystallizes in the orthorhombic space group P2(1)2(1)2(1). The adamantyl group is inserted into the cyclodextrin cavity of the adjacent molecule, entering by the side of the secondary hydroxy rim, thus forming a supramole... |
15084232 | Phyto-oestrogens and breast cancer chemoprevention. | Phytoestrogens are polyphenol compounds of plant origin that exhibit a structural similarity to the mammalian steroid hormone 17beta-oestradiol. In Asian nations the staple consumption of phyto-oestrogen-rich foodstuffs correlates with a reduced incidence of breast cancer. Human dietary intervention trials have noted a... |
15069487 | Engineering silver(I) coordination networks through hydrogen bonding. | The silver(I) coordination networks [Ag2(mu-O2CCF3)2(mu-NN)2](infinity) exist as a polymer of macrocycles or a double-stranded polymer when NN = 1,2-C6H4[NHC(O)-4-C5H4N]2 or 1,2-C6H4[NHC(O)-3-C5H4N]2, respectively. Crystal engineering of the polymers is achieved through interchain hydrogen bonds. |
15028630 | Cholesterol efflux alters lipid raft stability and distribution during capacitation of boar spermatozoa. | A reduction in plasma membrane cholesterol is one of the early events that either triggers or is closely associated with capacitation of mammalian spermatozoa. In this investigation, we have examined the effects of cholesterol efflux on tyrosine phosphorylation, lipid diffusion, and raft organization in boar spermatozo... |
15028716 | On the mechanism and significance of ligand-induced internalization of human neutrophil chemokine receptors CXCR1 and CXCR2. | It is well established that leukocyte chemotactic receptors, a subset of G protein-coupled receptors, undergo endocytosis after stimulation by ligand. However, the significance of this phenomenon to cell motility and other important leukocyte functions induced by chemoattractants has not been clearly defined. Here we s... |
15075240 | Troponin I is required for myofibrillogenesis and sarcomere formation in Drosophila flight muscle. | Myofibrillar proteins assemble to form the highly ordered repetitive contractile structural unit known as a sarcomere. Studies of myogenesis in vertebrate cell culture and embryonic developmental systems have identified some of the processes involved during sarcomere formation. However, isoform changes during vertebrat... |
15024426 | N-terminal phosphorylation of the dopamine transporter is required for amphetamine-induced efflux. | Amphetamine (AMPH) elicits its behavioral effects by acting on the dopamine (DA) transporter (DAT) to induce DA efflux into the synaptic cleft. We previously demonstrated that a human DAT construct in which the first 22 amino acids were truncated was not phosphorylated by activation of protein kinase C, in contrast to ... |
15069481 | Symmetry induced supramolecular assembly of a resorcinarene trimeric molecular box. | A novel trimeric resorcinarene molecular box is induced during hydrothermal synthesis by use of the 3-fold symmetric tripyridyl triazine linker molecule. |
15034560 | Stimuli-responsive polypeptide vesicles by conformation-specific assembly. | In biology, lipids are well known for their ability to assemble into spherical vesicles. Proteins, in particular virus capsids, can also form regular vesicle-like structures, where the precise folding and stable conformations of many identical subunits directs their self-assembly. Functionality present on these subunit... |
15024385 | p27 binds cyclin-CDK complexes through a sequential mechanism involving binding-induced protein folding. | p27 controls cell proliferation by binding and regulating nuclear cyclin-dependent kinases (CDKs). In addition, p27 interacts with other nuclear and cytoplasmic targets and has diverse biological functions. We seek to understand how the structural and dynamic properties of p27 mediate its several functions. We show tha... |
15069475 | Self-assembly and magnetic properties of a double-propeller octanuclear copper(II) complex with a meso-helicate-type metallacryptand core. | An octanuclear copper(II) complex possessing a dimer-of-tetramers structure self-assembles from a binuclear oxamatocopper(II) metallacryptand of the meso-helicate type; its magnetic behaviour is consistent with its unique double-propeller molecular topology. |
15020763 | Structural basis for HLA-DQ2-mediated presentation of gluten epitopes in celiac disease. | Celiac disease, also known as celiac sprue, is a gluten-induced autoimmune-like disorder of the small intestine, which is strongly associated with HLA-DQ2. The structure of DQ2 complexed with an immunogenic epitope from gluten, QLQPFPQPELPY, has been determined to 2.2-A resolution by x-ray crystallography. The glutamat... |
15020707 | The maize ID1 flowering time regulator is a zinc finger protein with novel DNA binding properties. | The INDETERMINATE protein, ID1, plays a key role in regulating the transition to flowering in maize. ID1 is the founding member of a plant-specific zinc finger protein family that is defined by a highly conserved amino sequence called the ID domain. The ID domain includes a cluster of three different types of zinc fing... |
15016646 | Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein. | A novel coronavirus, severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A panel of S protein-derived p... |
15071203 | Poly[[aquaneodymium(III)]-micro(3)-decane-1,10-dicarboxylato-micro(3)-9-carboxynonanecarboxylato]. | The title compound, [Nd(C(10)H(16)O(4))(C(10)H(17)O(4))(H(2)O)](n), has a novel Nd-organic framework constructed from sebacic acid (C(10)H(18)O(4)) linkers, the longest aliphatic ligand used to date in lanthanide metal-organic framework compounds. The structure contains edge-shared chains of NdO(8)(H(2)O) tricapped tri... |
15071202 | A two-dimensional network of R(2)(2)(16) and R(4)(2)(8) rings in trans-bis(4-aminopyridine-kappaN(1))bis(benzoato-kappa(2)O,O')copper(II) benzene 0.75-solvate. | The structure of the title supramolecular complex, [Cu(C(7)H(5)O(2))(2)(C(5)H(6)N(2))(2)].0.75C(6)H(6), has been determined. The Cu(2+) ion lies on an inversion centre and is coordinated by four O atoms of two opposing benzoate molecules and two pyridine N atoms of two opposing aminopyridine molecules. The partially oc... |
15071199 | 3-(4-cyanophenyl)pentane-2,4-dione and its copper(II) complex. | The beta-diketone 3-(4-cyanophenyl)pentane-2,4-dione crystallizes as the enol tautomer 4-(2-hydroxy-4-oxopent-2-en-3-yl)benzonitrile, C(12)H(11)NO(2), (I), with an intramolecular O-H.O hydrogen bond [O.O = 2.456 (2) A]. Reaction of (I) with copper acetate monohydrate in the presence of triethylamine leads to the format... |
15016920 | Modulation of the bilayer thickness of exocytic pathway membranes by membrane proteins rather than cholesterol. | A biological membrane is conceptualized as a system in which membrane proteins are naturally matched to the equilibrium thickness of the lipid bilayer. Cholesterol, in addition to lipid composition, has been suggested to be a major regulator of bilayer thickness in vivo because measurements in vitro have shown that cho... |
15016833 | A novel marker of tissue junctions, collagen XXII. | Here we describe a novel specific component of tissue junctions, collagen XXII. It was first identified by screening an EST data base and subsequently expressed as a recombinant protein and characterized as an authentic tissue component. The COL22A1 gene on human chromosome 8q24.2 encodes a collagen that structurally b... |
15016831 | Hyaluronan cell surface binding is induced by type I collagen and regulated by caveolae in glioma cells. | Hyaluronan (HA) is a component of the brain extracellular matrix environment that is synthesized and secreted by glioma cells. The primary cell surface receptor for HA is CD44, a membrane glycoprotein that is functionally regulated by a membrane type 1 matrix metalloproteinase (MT1-MMP). Both CD44 and MT1-MMP are parti... |
15252654 | Fused pyridazines: rigid multidentates for designing and fine-tuning the structure of hybrid organic/inorganic frameworks. | Fused pyridazines (1,2,3,6,7,8-hexahydro-cinnolino[5,4,3-cde]cinnoline, L and its 2,2,7,7-tetramethyl derivative, Me4L) are designed as rigid multidentate ligands for the construction of framework solids. In combination with copper(I) bromide (iodide) they provide excellent structural examples for predictive engineerin... |
15010548 | Heme as an optical probe of a conformational transition of ovine recPrP. | The prion protein occurs as a globular domain and a leading fragment whose structure is not well-defined. For the ovine species, all of the tryptophan residues are in the initial fragment, while the globular domain is rich in tyrosine residues. Using heme as a spectroscopic probe, we have studied the recombinant prion ... |
15010542 | Energy functions for protein design I: efficient and accurate continuum electrostatics and solvation. | Electrostatics and solvation energies are important for defining protein stability, structural specificity, and molecular recognition. Because these energies are difficult to compute quickly and accurately, they are often ignored or modeled very crudely in computational protein design. To address this problem, we have ... |
15010541 | Equilibrium thermal transitions of collagen model peptides. | The folding of collagen in vitro is very slow and presents difficulties in reaching equilibrium, a feature that may have implications for in vivo collagen function. Peptides serve as good model systems for examining equilibrium thermal transitions in the collagen triple helix. Investigations were carried out to ascerta... |
15010525 | Isolation of an endotoxin-MD-2 complex that produces Toll-like receptor 4-dependent cell activation at picomolar concentrations. | Host proinflammatory responses to minute amounts of endotoxins derived from many Gram-negative bacteria require the interaction of lipopolysaccharide-binding protein (LBP), CD14, Toll-like receptor 4 (TLR4) and MD-2. Optimal sensitivity to endotoxin requires an ordered series of endotoxin-protein and protein-protein in... |
15010456 | Caveolar and lipid raft localization of the growth hormone receptor and its signaling elements: impact on growth hormone signaling. | The growth hormone receptor (GHR) is a cell surface receptor that mediates the somatogenic and metabolic effects of the growth hormone (GH). GHR signaling is transduced via the receptor-associated cytoplasmic tyrosine kinase called Janus protein kinase 2 (JAK2). The major intracellular signaling systems activated by JA... |
15007069 | Nitric oxide inhibition of adenylyl cyclase type 6 activity is dependent upon lipid rafts and caveolin signaling complexes. | Several cell types, including cardiac myocytes and vascular endothelial cells, produce nitric oxide (NO) via both constitutive and inducible isoforms of NO synthase. NO attenuates cardiac contractility and contributes to contractile dysfunction in heart failure, although the precise molecular mechanisms for these effec... |
15007173 | Cholesterol depletion induces PKA-mediated basolateral-to-apical transcytosis of the scavenger receptor class B type I in MDCK cells. | Cholesterol-based membrane microdomains, or lipid rafts, are believed to play important, yet poorly defined, roles in protein trafficking and signal transduction. In polarized epithelial cells, the current view is that rafts are involved in apical but not in basolateral protein transport from the trans-Golgi network (T... |
15004551 | Steering molecular organization and host-guest interactions using two-dimensional nanoporous coordination systems. | Metal-organic coordination networks (MOCNs) have attracted wide interest because they provide a novel route towards porous materials that may find applications in molecular recognition, catalysis, gas storage and separation. The so-called rational design principle-synthesis of materials with predictable structures and ... |
15252677 | From the tetra(amino) phosphonium cation, [P(NHPh)4]+, to the tetra(imino) phosphate trianion, [P(NPh)4]3-, two-faced ligands that bind anions and cations. | The tetraanilino phosphonium cation, [P(N(H)Ph)4]+, 1+, is sequentially deprotonated by Bu(n)Li in thf. The deprotonation reaction of the chloride derivative, Cl, was monitored by (31)P NMR, which revealed the successive formation of the neutral [P(N(H)Ph)3(NPh)], 2, the monoanionic [P(N(H)Ph)2(NPh)2]-, 3-, the dianion... |
15004227 | Dynamic regulation of a GPCR-tetraspanin-G protein complex on intact cells: central role of CD81 in facilitating GPR56-Galpha q/11 association. | By means of a variety of intracellular scaffolding proteins, a vast number of heterotrimeric G protein-coupled receptors (GPCRs) may achieve specificity in signaling through a much smaller number of heterotrimeric G proteins. Members of the tetraspanin family organize extensive complexes of cell surface proteins and th... |
15004222 | An assembly-incompetent mutant establishes a requirement for dynamin self-assembly in clathrin-mediated endocytosis in vivo. | Dynamin GTPase activity is required for its biological function in clathrin-mediated endocytosis; however, the role of self-assembly has not been unambiguously established. Indeed, overexpression of a dynamin mutant, Dyn1-K694A, with impaired ability to self-assemble has been shown to stimulate endocytosis in HeLa cell... |
15198508 | Beta-cyclodextrin complexes of celecoxib: molecular-modeling, characterization, and dissolution studies. | Celecoxib, a specific inhibitor of cycloxygenase-2 (COX-2) is a poorly water-soluble nonsteroidal anti-inflammatory drug with relatively low bioavailability. The effect of beta-cyclodextrin on the aqueous solubility and dissolution rate of celecoxib was investigated. The possibility of molecular arrangement of inclusio... |
15004029 | Extracellular bone acidic glycoprotein-75 defines condensed mesenchyme regions to be mineralized and localizes with bone sialoprotein during intramembranous bone formation. | Bone acidic glycoprotein-75 is expressed very early during in vivo models of intramembranous bone formation, highly enriched in condensing osteogenic mesenchyme after marrow ablation and the osteoprogenitor layer of tibial periosteum. Bone sialoprotein accumulates within bone acidic glycoprotein-75-enriched matrix area... |
15089497 | Quantum magneto-oscillations in a supramolecular Mn(II)-[3 x 3] grid. | The magnetic grid molecule Mn(II)-[3 x 3] has been studied by high-field torque magnetometry at 3He temperatures. At fields above 5 T, the torque versus field curves exhibit an unprecedented oscillatory behavior. A model is proposed which describes these magneto-oscillations well. |
15045109 | A mixed-bridging ligand nonanuclear Ru(II) dendrimer containing a tris- chelating core. Synthesis and redox properties. | The first ligand-cored dendrimer based on branching Ru(II) centers and containing mixed polypyridine bridging ligands has been prepared; redox experiments suggest that the redox-active core is not reduced at the expected potential, probably as a consequence of shielding induced by the rigid dendritic array. |
15001562 | Membrane cholesterol interferes with neuronal apoptosis induced by soluble oligomers but not fibrils of amyloid-beta peptide. | Neuronal cell death in Alzheimer's disease (AD) is partly induced by the interaction of the amyloid-beta peptide (Abeta) with the plasma membrane of target cells. Accordingly, recent studies have suggested that cholesterol, an important component of membranes that controls their physical properties and functions, plays... |
15001708 | Docking of cytosolic chaperone-substrate complexes at the membrane ATPase during flagellar type III protein export. | Bacterial type III protein export underlies flagellum assembly and delivery of virulence factors into eukaryotic cells. The sequence of protein interactions underlying the export pathway are poorly characterized; in particular, it is not known how chaperoned substrates in the cytosol are engaged by the membrane-localiz... |
15001706 | Protein dynamics and the immunological evolution of molecular recognition. | While it is accepted that protein flexibility plays a role in protein folding, catalysis, and molecular recognition, few techniques are capable of the rigorous measurement of protein motions required to quantify flexibility. Three-pulse photon echo shift spectroscopy can be used to measure the time scale of protein mot... |
14998921 | Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development. | Basement membranes are specialized extracellular matrices consisting of tissue-specific organizations of multiple matrix molecules and serve as structural barriers as well as substrates for cellular interactions. The network of collagen IV is thought to define the scaffold integrating other components such as, laminins... |
14997356 | Size and composition of membrane protein clusters predicted by Monte Carlo analysis. | Biological membranes contain a high density of protein molecules, many of which associate into two-dimensional microdomains with important physiological functions. We have used Monte Carlo simulations to examine the self-association of idealized protein species in two dimensions. The proteins have defined bond strength... |
14996913 | Ganglioside GM1 levels are a determinant of the extent of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus. | Cholera toxin is associated with caveolae and raft domains in various cell types and previous studies have shown that cholera toxin can be internalized by caveolae/raft-dependent endocytosis as well as by other pathways. We undertook the study of cholera toxin endocytosis in CaCo-2 and HeLa cells. CaCo-2 cells do not e... |
15004280 | The quantal nature of Ca2+ sparks and in situ operation of the ryanodine receptor array in cardiac cells. | Intracellular Ca(2+) release in many types of cells is mediated by ryanodine receptor Ca(2+) release channels (RyRCs) that are assembled into two-dimensional paracrystalline arrays in the endoplasmic/sarcoplasmic reticulum. However, the in situ operating mechanism of the RyRC array is unknown. Here, we found that the e... |
18969351 | Solvent extraction of silver picrate by 3m-crown-m ethers (m = 5, 6) and its mono-benzo-derivative from water into benzene or chloroform: elucidation of an extraction equilibrium using component equilibrium constants. | Ion-pair formation constant (K(AgPic) in mol(-1)dm(3)) of silver picrate (AgPic), those (K(AgLPic)) of its ion-pair complexes (AgLPic) with crown ethers (L) and complex formation constants (K(AgL)) of Ag(+) with L (15-crown-5 ether (15C5) and benzo-15C5) in water (w) were determined potentiometrically at 25 degrees C. ... |
18969350 | Fast determination of flavonoids in Hippophae rhamnoides and its medicinal preparation by capillary zone electrophoresis using dimethyl-beta-cyclodextrin as modifier. | A fast capillary zone electrophoresis (CZE) method, using dimethyl-beta-cyclodextrin (DM-beta-CD) as modifier, has been developed for the determination of three flavonoids (quercetin (QU), kaempferol (KA) and isorhamnetin (IS)) in the Chinese herbal extract from Hippophae rhamnoides and its medicinal preparation (Sinda... |
17191861 | Associating hyaluronan derivatives: a novel horizon in viscosupplementation of osteoarthritic joints. | The association of two high-molecular-weight hyaluronan (HA) derivatives, namely a beta-cyclodextrin (HA-beta-CD) and an N-acylurea (EDC-HA), dissolved in aqueous NaCl, was studied. The weight-average of the molecular weights (Mw) of HA-beta-CD and of EDC-HA was 185.3 and 86.8 kDa, respectively. However, the Mw value d... |
17191863 | Diastereoisomer-selective inclusion complexation of Cinchona alkaloids with a modified beta-cyclodextrin: fluorescent behavior enhanced by chiral-tether binding. Short communication. | The molecular 1:1 complexation of cinchona alkaloids by mono(6-deoxy-6-{[(R)-1-(hydroxymethyl)propyl]amino})-beta-cyclodextrin (1) in aqueous solution has been investigated by 2D-NMR, fluorescence titration, and fluorescence-lifetime experiments. Generally, with 1 as the host, in contrast to beta-cyclodextrin proper, s... |
15832513 | Solution structures of 1:1 complexes of oxyphenonium bromide with beta- and gamma-cyclodextrins. | The solution structures of complexes of oxyphenonium bromide (OB) with beta- and gamma-cyclodextrins (beta- and gamma-CDs, respectively) in deuterium oxide have been investigated by 500 MHz proton NMR spectroscopy and molecular mechanics calculations. The chemical shifts induced by complex formation provide the 1:1 bin... |
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