id stringlengths 4 8 | title stringlengths 1 642 | abstract stringlengths 50 17.9k |
|---|---|---|
7724525 | High incidence of XXY and XYY males among the offspring of female chimeras from embryonic stem cells. | Injecting male embryonic stem cells into the blastocoel of female embryos occasionally produces female chimeras capable of transmitting the embryonic stem cell genome. In our experiments several embryonic stem cell-derived male offspring from female chimeras were observed to be infertile. Karyotypic analysis of these i... |
7536925 | Proline-rich sequences that bind to Src homology 3 domains with individual specificities. | To study the binding specificity of Src homology 3 (SH3) domains, we have screened a mouse embryonic expression library for peptide fragments that interact with them. Several clones were identified that express fragments of proteins which, through proline-rich binding sites, exhibit differential binding specificity to ... |
7722627 | Cyclooxygenase inhibition and the spinal release of prostaglandin E2 and amino acids evoked by paw formalin injection: a microdialysis study in unanesthetized rats. | Injection of formalin into the hind paw evokes a biphasic flinching of the injured paw. Pharmacological characterization of this behavior has implicated the spinal release of excitatory amino acids (EAAs) and cyclooxygenase (COX) products. To address this hypothesis, we examined the effect of paw formalin injection on ... |
7720710 | High mobility group protein 2 functionally interacts with the POU domains of octamer transcription factors. | The octamer transcription factors Oct1 and Oct2 are involved in the transcriptional regulation of both lymphoid-specific and ubiquitously expressed genes. Their activity depends critically on their interaction with distinct cellular cofactors. Therefore, we have isolated cDNAs encoding proteins that physically interact... |
7720709 | RAR-specific agonist/antagonists which dissociate transactivation and AP1 transrepression inhibit anchorage-independent cell proliferation. | Using retinoic acid receptor (RAR) reporter cells specific for either RAR alpha, beta or gamma, we have identified synthetic retinoids which specifically induce transactivation by RAR beta, while antagonizing RA-induced transactivation by RAR alpha and RAR gamma. Like RA, these synthetic retinoids allow all three RAR t... |
7719472 | Study of the enantioselective binding between BOF-4272 and serum albumins by means of high-performance frontal analysis. | High-performance frontal analysis (HPFA) was incorporated in an on-line HPLC system for the study of the enantioselective binding of BOF-4272, a new xanthine oxidase inhibitor, with human, bovine and rat serum albumins. This HPLC system consists of a HPFA column (diol-silica column), an extraction column (C4 column) an... |
7719463 | Unified enantioselective capillary chromatography on a Chirasil-DEX stationary phase. Advantages of column miniaturization. | Immobilized Chirasil-DEX (mono-6-O-octamethylenepermethyl-beta-cyclodextrin chemically linked to dimethylpolysiloxane) can be employed as a versatile chiral stationary phase in chromatography. The chiral polymer has a long lifetime and is configurationally and thermally stable. The concept of unified enantioselective c... |
7708755 | Interaction of the anthracycline 4'-iodo-4'-deoxydoxorubicin with amyloid fibrils: inhibition of amyloidogenesis. | All types of amyloidosis are structurally characterized by the cross beta-pleated sheet conformation of the fibrils, irrespective of their biochemical composition. The clinical observation that the anthracycline 4'-iodo-4'-deoxy-doxorubicin (IDOX) can induce amyloid resorption in patients with immunoglobulin light chai... |
7708014 | Protein-protein interactions: methods for detection and analysis. | The function and activity of a protein are often modulated by other proteins with which it interacts. This review is intended as a practical guide to the analysis of such protein-protein interactions. We discuss biochemical methods such as protein affinity chromatography, affinity blotting, coimmunoprecipitation, and c... |
7707320 | Search for new purine- and ribose-modified adenosine analogues as selective agonists and antagonists at adenosine receptors. | The binding affinities at rat A1, A2a, and A3 adenosine receptors of a wide range of derivatives of adenosine have been determined. Sites of modification include the purine moiety (1-, 3-, and 7-deaza; halo, alkyne, and amino substitutions at the 2- and 8-positions; and N6-CH2-ring, -hydrazino, and -hydroxylamino) and ... |
7702586 | In vitro fibrillogenesis of collagen II from pig vitreous humour. | Collagen from pig vitreous humour was fractionated into a soluble and an insoluble fraction by centrifugation. Most of the collagen II in the soluble fraction was present as pN-collagen II (procollagen II without the C-terminal propeptide), besides smaller quantities of procollagen II, collagen II and two as yet uniden... |
7702582 | A stopped-flow apparatus for infrared spectroscopy of aqueous solutions. | IR spectroscopy has been widely applied in the study of photo-activated biological processes such as photosynthesis, but has not been applied to the study of reacting systems which require rapid mixing of aqueous solutions. This has been due in part to the high pressure needed to make an aqueous solution flow rapidly t... |
7702567 | Interaction of component enzymes with the peripheral subunit-binding domain of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus: stoichiometry and specificity in self-assembly. | The interaction between the pyruvate decarboxylase (E1) component and a di-domain (lipoyl domain plus peripheral subunit-binding domain) from the dihydrolipoyl acetyltransferase (E2) component of the Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex was investigated. Only 1 mol of di-domain (bindin... |
7535098 | Peptide ligands for integrin alpha v beta 3 selected from random phage display libraries. | The integrin alpha v beta 3 binds promiscuously to cell-adhesive proteins: vitronectin, fibronectin, and several others containing the RGD motif. We have explored molecular recognition by alpha v beta 3 through selection of ligands from large random libraries of peptides displayed on phage. Ligands bound by alpha beta ... |
7695909 | Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteins. | We are analyzing features of the K+ channel subunit proteins that are critical for function and regulation of these proteins. Our studies show biochemically that subunit proteins from the Shaker and Shaw subfamilies fail to assemble into a heteromultimer. The basis for this incompatibility is the sequences contained wi... |
7896879 | Microtubule polarity and dynamics in the control of organelle positioning, segregation, and cytokinesis in the trypanosome cell cycle. | Trypanosoma brucei has a precisely ordered microtubule cytoskeleton whose morphogenesis is central to cell cycle events such as organelle positioning, segregation, mitosis, and cytokinesis. We have defined microtubule polarity and show the + ends of the cortical microtubules to be at the posterior end of the cell. Meas... |
7896873 | Ezrin NH2-terminal domain inhibits the cell extension activity of the COOH-terminal domain. | Overexpression in insect cells of the full coding sequence of the human membrane cytoskeletal linker ezrin (1-586) was compared with that of a NH2-terminal domain (ezrin 1-233) and that of a COOH-terminal domain (ezrin 310-586). Ezrin (1-586), as well as ezrin (1-233) enhanced cell adhesion of infected Sf9 cells withou... |
7893700 | Variable-temperature spectroelectrochemical study of horseradish peroxidase. | The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed vi... |
7893684 | The energetics and dynamics of molecular recognition by calmodulin. | Amide hydrogen exchange has been used to examine the structural dynamics and energetics of the interaction of a peptide corresponding to the calmodulin binding domain of smooth muscle myosin light chain kinase with calcium-saturated calmodulin. Heteronuclear NMR 15N-1H correlation techniques were used to quantitate ami... |
7893661 | Self-assembly of DNA oligomers into high molecular weight species. | Thermal denaturation, gel electrophoresis, and circular dichroism methods were used to characterize DNA oligomers possessing one or two segments of four contiguous G bases in order to investigate their environmentally dependent conformational properties. The sequences of the oligomers studied were the following: HP1-T ... |
7892246 | Selection of peptide inhibitors of interactions involved in complex protein assemblies: association of the core and surface antigens of hepatitis B virus. | As an example for studies of contacts involved in complex biological systems, peptide ligands that bind to the core antigen of hepatitis B virus (HBcAg) have been selected from a random hexapeptide library displayed on filamentous phage. Affinity-purified phage bearing aa sequence LLGRMK, or some related sequences, bou... |
7892244 | Enhanced activity of the bacteriophage lambda PL promoter at low temperature. | The response of the early phage lambda PL promoter to temperature was investigated. Experiments with lacZ reporter gene fusions demonstrated that the activity of the phage lambda PL promoter is inversely dependent on temperature. The bacterial DNA-binding protein integration host factor (IHF) further enhances lambda PL... |
7534342 | Tenascin knockout mice: barrels, boundary molecules, and glial scars. | In light of a previous report suggesting that the brains of tenascin-deficient animals are grossly normal, we have studied the somatosensory cortical barrel field and injured cerebral cortex in postnatal homozygous tenascin knockout, heterozygote, and normal wild-type mice. Nissl staining, cytochrome oxidase, and Dil a... |
7891144 | Structural plasticity in the Drosophila brain. | The Drosophila brain is highly variable in size. Female flies grown in densely populated larval cultures have up to 20% more Kenyon cell fibers in their mushroom bodies than flies from low-density cultures. These differences in the number of Kenyon cell fibers are accompanied by differences in the volume of the calyx. ... |
7887894 | Multiple native-like conformations trapped via self-association-induced hydrophobic collapse of the 33-residue beta-sheet domain from platelet factor 4. | Native platelet factor 4 (PF4) (70 residues) has a hydrophobic three-stranded anti-parallel beta-sheet domain on to which is folded an amphipathic C-terminal alpha-helix and an aperiodic N-terminal domain. The 33-amino acid beta-sheet domain from PF4 (residues 23-55) has been synthesized and studied by c.d. and n.m.r. ... |
7883725 | Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component. | The recombinant CelS (rCelS), the most abundant catalytic subunit of the Clostridium thermocellum cellulosome, displayed typical exoglucanase characteristics, including (i) a preference for amorphous or crystalline cellulose over carboxymethyl cellulose, (ii) an inability to reduce the viscosity of a carboxymethyl cell... |
7883713 | Cloning, sequencing, and expression of the uroporphyrinogen III methyltransferase cobA gene of Propionibacterium freudenreichii (shermanii). | We cloned, sequenced, and overexpressed cobA, the gene encoding uroporphyrinogen III methyltransferase in Propionibacterium freudenreichii, and examined the catalytic properties of the enzyme. The methyltransferase is similar in mass (27 kDa) and homologous to the one isolated from Pseudomonas denitrificans. In contras... |
7533757 | The S-layer from Bacillus stearothermophilus DSM 2358 functions as an adhesion site for a high-molecular-weight amylase. | The S-layer lattice from Bacillus stearothermophilus DSM 2358 completely covers the cell surface and exhibits oblique symmetry. During growth of B. stearothermophilus DSM 2358 on starch medium, three amylases with molecular weights of 58,000, 98,000, and 184,000 were secreted into the culture fluid, but only the high-m... |
7880832 | Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity. | Tyrosine 195 is located in the center of the active site cleft of cyclodextrin glycosyltransferase (EC 2.4.1.19) from Bacillus circulans strain 251. Alignment of amino acid sequences of CGTases and alpha-amylases, and the analysis of the binding mode of the substrate analogue acarbose in the active site cleft [Strokopy... |
7877692 | A structural basis of the interactions between leucine-rich repeats and protein ligands. | The leucine-rich repeat is a recently characterized structural motif used in molecular recognition processes as diverse as signal transduction, cell adhesion, cell development, DNA repair and RNA processing. We present here the crystal structure at 2.5 A resolution of the complex between ribonuclease A and ribonuclease... |
7877231 | Platelet count and the outcome of operation for ruptured abdominal aortic aneurysm. | <h4>Purpose</h4>The purpose of this study was to determine the relationship between admission and postoperative platelet count (PC) and mortality and morbidity rates after emergency surgery for ruptured abdominal aortic aneurysm (AAA).<h4>Methods</h4>We performed a case record review of 65 consecutive patients admitted... |
7877175 | Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution. | The three-dimensional structure of the calcium-free form of Bacillus licheniformis alpha-amylase (BLA) has been determined by multiple isomorphous replacement in a crystal of space group P4(3)2(1)2 (a = b = 119.6 A, c = 85.4 A). The structure was refined using restrained crystallographic refinement to an R-factor of 0.... |
7876306 | A posttranslational modification of beta-actin contributes to the slow dissociation of the spectrin-protein 4.1-actin complex of irreversibly sickled cells. | Irreversibly sickled cells (ISCs) remain sickled even under conditions where they are well oxygenated and hemoglobin is depolymerized. In our studies we demonstrate that triton extracted ISC core skeletons containing only spectrin, protein 4.1, and actin also retain their sickled shape; while reversibly sickled cell (R... |
7876227 | Identification of the tryptophan residue in the thiamin pyrophosphate binding site of mammalian pyruvate dehydrogenase. | The pyruvate dehydrogenase (E1) component of the mammalian pyruvate dehydrogenase complex catalyzes the oxidative decarboxylation of pyruvate with the formation of an acetyl residue and reducing equivalents, which are transferred sequentially to the dihydrolipoyl acetyltransferase and dihydrolipoamide dehydrogenase com... |
7874343 | A multicenter study of phospholipase A2 in patients in intensive care units. | <h4>Background</h4>Multiple organ failure (MOF) is becoming the most common cause of death in patients in surgical intensive care units. In this respect, a multicenter study in four different groups of patients in surgical intensive care units was done to evaluate the role of phospholipase A2 (PLA2) in comparison with ... |
7868585 | Characterization of the protein conferring immunity to the antimicrobial peptide carnobacteriocin B2 and expression of carnobacteriocins B2 and BM1. | Cloning of a 16-kb DNA fragment from the 61-kb plasmid of Carnobacterium piscicola LV17B into plasmidless C. piscicola LV17C restores the production of the plasmid-encoded carnobacteriocin B2 and the chromosomally-encoded carnobacteriocin BM1 and restores the immune phenotype. This fragment also has sufficient genetic ... |
7868237 | Expression of a nonagglutinating fimbria by Proteus mirabilis. | We have clarified growth conditions and isolation strategies for the nonagglutinating fimbriae from Proteus mirabilis. Nonagglutinating fimbriae were expressed by all P. mirabilis strains we examined, and the major subunit proteins, which ranged from 23 to 29 kDa as determined by sodium dodecyl sulfate-polyacrylamide g... |
7862165 | Targeted mutagenesis in mammalian cells mediated by intracellular triple helix formation. | As an alternative to standard gene transfer techniques for genetic manipulation, we have investigated the use of triple helix-forming oligonucleotides to target mutations to selected genes within mammalian cells. By treating monkey COS cells with oligonucleotides linked to psoralen, we have generated targeted mutations... |
7862153 | A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. | To understand the mechanisms involved in homologous recombination, we have performed a search for Saccharomyces cerevisiae mutants unable to carry out plasmid-to-chromosome gene conversion. For this purpose, we have developed a colony color assay in which recombination is induced by the controlled delivery of double-st... |
7853539 | Contribution of conserved amino acids in mediating the interaction between EBNA2 and CBF1/RBPJk. | The Epstein-Barr virus EBNA2 protein is a transcriptional activator that achieves promoter specificity through interaction with the cellular DNA-binding protein CBF1/RBPJk. Within the amino acid 252-to-425 EBNA2 domain that targets CBF1/RBPJk lie three amino acid clusters, conserved regions (CR) 5, 6, and 7, that are r... |
24394260 | The interplay between nutrition and body composition. | Stress and malnutrition are associated with altered body composition. Extracellular fluid increases, with wt gain, but in response to stress BCM may gradually shrink with wt loss. In catabolic illness there is extracellular fluid expansion and erosion of AT and BCM. In stress, net loss of body fat was associated with i... |
17020854 | Molecular dynamics of amphotericin B I. Single molecule in vacuum and water. | Molecular dynamics simulations were performed for an antifungal polyene antibiotic amphotericin B (AMB). A single molecule of the AMB was modeled in vacuum as well as in water. In the latter case it was surrounded by 354 SPC water molecules and a periodic boundary condition was applied. An amino-sugar mycosamine ring w... |
8549903 | [Observed vs expected mortality in the emergency service of a general hospital. Evaluation by the APACHE II and multiple organ failure classification systems]. | <h4>Unlabelled</h4>Problem that originated the investigation: It was not known if the observed mortality in the Emergency room of the Tijuana General Hospital was lower, equal or higher, than the expected mortality.<h4>Objectives</h4>1) Evaluate in a prospective fashion the mortality rate in that hospital service, and ... |
7556801 | Regeneration of the tunic cuticle in the compound ascidian, Botrylloides simodensis. | The tunic cuticle of Botrylloides simodensis, a colonial ascidian, is an electrondense sheet overlaying the tunic in which zooids are embedded. When the margins of colonies were cut off and the colonies were incubated in running seawater, the tunic cuticle regenerated at the cut surface of the tunic matrix by the follo... |
7630860 | Improvement of ursodeoxycholic acid bioavailability by 2-hydroxypropyl-beta-cyclodextrin complexation in healthy volunteers. | Tablets containing the inclusion complex of ursodeoxycholic acid (UDCA) with 2-hydroxypropyl-beta-cyclodextrin were prepared by direct compression. Plasma concentrations of UDCA were determined in six healthy volunteers after oral administration of tablets containing the inclusion complex or UDCA alone (Ursacol). Follo... |
7620708 | Role of angiotensin converting enzyme in the vascular effects of an endopeptidase 24.15 inhibitor. | 1. We investigated the role of angiotensin converting enzyme (ACE) in the cardiovascular effects of N-[1-(R,S)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate (cFP), a peptidase inhibitor selective for metalloendopeptidase (EP) E.C. 3.4.24.15. 2. In conscious rabbits, cFP (5 mg kg-1, i.v.) markedly slowed the degra... |
7620705 | Characterization of [3H]-imidazenil binding to rat brain membranes. | 1. The binding of [3H]-imidazenil, an imidazobenzodiazepine carboxamide, to rat cerebellar membranes was characterized at different temperatures. 2. Specific binding was linear with tissue concentrations and reached maximum after 90, 30 and 5 min incubation at 0, 21 and 37 degrees C, respectively. The binding was of hi... |
7616373 | Theoretical studies on the structures of natural and alkylated cyclodextrins. | A series of semiempirical molecular orbital calculations using the AM1 method have been performed on isolated natural and alkylated alpha- and beta-cyclodextrins. For natural cyclodextrins, three geometries were considered: (i) molecular mechanics (MM2); (ii) AM1 fully optimized geometry; (iii) X-ray structures based o... |
7616366 | beta-cyclodextrin derivatives, SBE4-beta-CD and HP-beta-CD, increase the oral bioavailability of cinnarizine in beagle dogs. | The absolute bioavailabilities (Fabs) of cinnarizine after oral administration as two modified beta-cyclodextrin (SBE4-beta-CD or HP-beta-CD) solutions, an aqueous suspension, and two capsules in fasted beagle dogs were determined. Cinnarizine was administered orally (25.0 mg) and intravenously (12.5 mg) to four dogs. ... |
7614909 | New injectable aqueous carbamazepine solution through complexing with 2-hydroxypropyl-beta-cyclodextrin: tolerability and pharmacokinetics after intravenous injection in comparison to a glycofurol-based formulation. | The poor water solubility of the antiepileptic drug (AED) carbamazepine (CBZ) is generally considered an absolute contraindication to parenteral administration in epileptic patients. However, the water solubility of CBZ can be largely enhanced through formation of an inclusion complex with an amorphous cyclodextrin der... |
7599265 | Yttrium-90 chelation properties of tetraazatetraacetic acid macrocycles, diethylenetriaminepentaacetic acid analogues, and a novel terpyridine acyclic chelator. | Realization of the potential of yttrium-90 for the radioimmunotherapy of cancer depends on rapid and kinetically stable chelation. Conditions were evaluated that influenced the chelation efficiency of these select chelators for yttrium-90: the macrocyclic chelators 2-(rho-nitrobenzyl)-1,4,7,10-tetraazacyclododecane-N,N... |
7598230 | Rapid and slow hydroxylators of seminal E prostaglandins among men in barren unions. | E prostaglandins are formed in seminal vesicles and can be oxygenated by (omega-1)-hydroxylation catalysed by cytochrome P450 to 19(R)-hydroxy metabolites. The latter are not further metabolized. Prostaglandin E1 (PGE1), PGE2, 19-hydroxy-PGE1 and 19-hydroxy-PGE2 were measured in seminal fluid of 95 men, who attended th... |
7596284 | Differential DNA bending introduced by the Pseudomonas putida LysR-type regulator, CatR, at the plasmid-borne pheBA and chromosomal catBC promoters. | The plasmid-borne pheBA operon of Pseudomonas putida strain PaW85 allows growth of the host cells on phenol. The promoter of this operon is activated by the chromosomally encoded LysR-type regulator CatR, in the presence of the inducer cis,cis-muconate. cis,cis-muconate is an intermediate of catechol degradation by the... |
7795783 | Enantioselective determination of thiamylal in human serum by high-performance liquid chromatography. | Thiamylal, a widely used anesthetic drug, has two enantiomers. We developed a novel and simple method for measuring thiamylal enantiomers in human serum using reversed-phase high-performance liquid chromatography. R(+)- and S(-)-Thiamylal were separated using a chiral mobile phase containing beta-cyclodextrin, and dete... |
7795782 | High-performance liquid chromatographic determination of (S)- and (R)-propranolol in human plasma and urine with a chiral beta-cyclodextrin bonded phase. | The determination of propranolol enantiomers in microsamples of human plasma and urine by HPLC using a chiral stationary phase is described. After extraction from 200 microliters of plasma or urine with racemic alprenolol as internal standard (I.S.), the enantiomers are separated on a beta-cyclodextrin column with a po... |
7795531 | Stabilization of a strained protein loop conformation through protein engineering. | Staphylococcal nuclease is found in two folded conformations that differ in the isomerization of the Lys 116-Pro 117 peptide bond, resulting in two different conformations of the residue 112-117 loop. The cis form is favored over the trans with an occupancy of 90%. Previous mutagenesis studies have shown that when Lys ... |
7795530 | A proposed structural model of domain 1 of fasciclin III neural cell adhesion protein based on an inverse folding algorithm. | Fasciclin III is an integral membrane protein expressed on a subset of axons in the developing Drosophila nervous system. It consists of an intracellular domain, a transmembrane region, and an extracellular region composed of three domains, each predicted to form an immunoglobulin-like fold. The most N-terminal of thes... |
7795529 | Refined solution structure of human profilin I. | Profilin is a ubiquitous eukaryotic protein that binds to both cytosolic actin and the phospholipid phosphatidylinositol-4,5-bisphosphate. These dual competitive binding capabilities of profilin suggest that profilin serves as a link between the phosphatidyl inositol cycle and actin polymerization, and thus profilin ma... |
7795522 | Conservative and nonconservative mutations in proteins: anomalous mutations in a transport receptor analyzed by free energy and quantum chemical calculations. | Experimental studies on a bacterial sulfate receptor have indicated anomalous relative binding affinities for the mutations Ser130-->Cys,Ser130-->Gly, and Ser130-->Ala. The loss of affinity for sulfate in the former mutation was previously attributed to a greater steric effect on the part of the Cys side chain relative... |
7795519 | Approaches to labeling and identification of active site residues in glycosidases. | Glycosidases play a key role in a number of biological processes and, as such, are of considerable clinical and biotechnological importance. Knowledge of the identifies of catalytically important active site residues is essential for understanding the catalytic mechanism, for enzyme classification, and for targeted bio... |
7795518 | Structural basis of substrate specificity in the serine proteases. | Structure-based mutational analysis of serine protease specificity has produced a large database of information useful in addressing biological function and in establishing a basis for targeted design efforts. Critical issues examined include the function of water molecules in providing strength and specificity of bind... |
7794597 | [Modification of the pharmacokinetics of some antibiotics by oral administration with an L-(-)verbenone beta-cyclodextrin complex]. | With the aim to evaluate the effect of L (-) Verbenone on the pharmacokinetic of some antibiotics, a compound has been prepared with beta cyclodextrin, allowing the administration per os. Pharmacological studies have been carried out using erythromycin and ampicillin, determining their kinetics in the hepatic and lung ... |
7793894 | Dimerization and membrane anchors in extracellular targeting of vancomycin group antibiotics. | Antibiotics of the vancomycin group are shown to enhance their affinities for the bacterial cell wall by the devices of either dimerization (vancomycin and other glycopeptides which dimerize even more strongly) or use of a membrane anchor (teicoplanin); a chelate mechanism is suggested in both cases, as supported by an... |
7793892 | SCH 51048, a new antifungal triazole active against hematogenous Candida krusei infections in neutropenic mice. | Candida krusei is increasingly recognized as an opportunistic pathogen in immunocompromised patients and is inherently resistant to fluconazole. We tested the in vivo efficacy of SCH 51048, an investigational antifungal triazole, in experimental hematogenous murine infection caused by two C. krusei isolates and compare... |
7793820 | Adjuvant chemotherapy for colorectal cancer. | Colorectal cancer is the second most common cancer in the Western world, and yet the survival after potentially curative excisional surgery has improved little over the last half century. Newer tumour prognostic markers are not superior to conventional Dukes' staging and there are currently no markers which predict res... |
7793287 | [Water uptake of tablets, their disintegration and dissolution are in close relation]. | Authors investigated the factors influencing this connection on phenylbutazone tablets. Tablets were prepared by wet granulation using three binders (gelatine, hydroxypropyl-cellulose, hydroxyethylcellulose) in different concentrations. Three disintegrants were compared (polyvinylpolypyrrolidon, formalin-casein and cyc... |
7792834 | A convenient synthesis of 7 alpha-hydroxycholest-4-en-3-one by the hydroxypropyl-beta-cyclodextrin-facilitated cholesterol oxidase oxidation of 3 beta,7 alpha-cholest-5-ene-3,7-diol. | The initial biosynthetic conversions of cholesterol to the bile acids involve sequential 7 alpha-hydroxylation (catalyzed by cholesterol 7 alpha-hydroxylase) followed by C-3 oxidation and concomitant double bond migration (to a delta 4-configuration, catalyzed by 3 beta-delta 5-C27-steroid oxidoreductase) to provide 7 ... |
7780814 | [The role of tumor necrosis factor in acute pancreatitis and associated multiple organ failure]. | In 32 patients with acute pancreatitis (AEP = 15, ANP = 17), tumor necrosis factor (TNF) activity, oxygen-derived free radicals (OFR) and endotoxin contents were determined by L929 cell bioassay, Tetrabarbituric acid fluorometry and Limulus test respectively. The results showed that TNF and OFR elevated in patients wit... |
7775976 | The neural cell adhesion molecule and synaptic plasticity. | Highly stereotyped patterns of neuronal connections are laid down during the development of the nervous system via a range of activity independent and activity dependent mechanisms. Whereas the coarse hard-wiring of the nervous system appears to rely on molecular recognition events between the neuron, its pathway, and ... |
7762821 | Determination and use of Rohrschneider-McReynolds constants for chiral stationary phases used in capillary gas chromatography. | The overall polarity of 22 chiral stationary phases (CSPs) used in capillary open tubular gas chromatography columns was estimated using the first five Rohrschneider-McReynolds constants. Most of the columns (i.e., 18) were wall-coated, and four were of the wall-immobilized or of the so-called "bonded" type. A wall-coa... |
7762799 | Charge-transfer chromatographic study of the complex formation of some anticancer drugs with gamma-cyclodextrin. | The interaction between 23 anticancer drugs and gamma-cyclodextrin (gamma-CD) was studied by reversed-phase charge-transfer thin-layer chromatography and the relative strength of interaction was calculated. gamma-CD formed inclusion complexes with 14 compounds, the complex always being more or less hydrophobic than the... |
7756548 | Computer modeling of electrostatic steering and orientational effects in antibody-antigen association. | Brownian dynamics simulations are performed to investigate the role of long-range electrostatic forces in the association of the monoclonal antibody HyHEL-5 with hen egg lysozyme. The electrostatic field of the antibody is obtained from a solution of the nonlinear Poisson-Boltzmann using the x-ray crystal coordinates o... |
7756545 | Interpretation of preferential interaction coefficients of nonelectrolytes and of electrolyte ions in terms of a two-domain model. | For a three-component system consisting of solvent (1), polymer or polyelectrolyte (2J), and a nonelectrolyte or electrolyte solute (3), a two-domain description is developed to describe thermodynamic effects of interactions between solute components (2J) and (3). Equilibrium dialysis, which for an electrolyte solute p... |
7756527 | Polarized Raman spectra of oriented fibers of A DNA and B DNA: anisotropic and isotropic local Raman tensors of base and backbone vibrations. | Polarized Raman spectra of oriented fibers of calf thymus DNA in the A and B conformations have been obtained by use of a Raman microscope operating in the 180 degrees back-scattering geometry. The following polarized Raman intensities in the spectral interval 200-1800 cm-1 were measured with both 514.5 and 488.0 nm la... |
7756525 | Mechanochemical study of NaDNA and NaDNA-netropsin fibers in ethanol-water and trifluoroethanol-water solutions. | Highly oriented calf-thymus NaDNA fibers, prepared by a wet-spinning method, were complexed with netropsin in ethanol-water and trifluoroethanol (TFE)-water solutions. The relative fiber length, L/L0, was measured at room temperature as a function of ethanol or TFE concentration to obtain information on the B-A conform... |
7754396 | [Effect of early intubation on the reduction of post-traumatic organ failure]. | In a prospective multiple trauma study lasting from 1986 to 1991 the influence of early intubation (EI) within 2 h after the accident on posttraumatic (multiple) organ failure (M)OF was compared with that of delayed intubation (DI) in 131 patients with multiple injuries (ISS 37). The indications for intubation were unc... |
7749568 | Localization of seed protein genes on metaphase chromosomes of Vicia faba via fluorescence in situ hybridization. | Using fluorescence in situ hybridization (FISH), four different seed protein genes were physically mapped on metaphase chromosomes of Vicia faba L. dropped on slides. FISH with a 2.8 kb genomic probe of a legumin B4 gene resulted in reproducible signals on the long arm of chromosome III near the centromere. The same cl... |
7749567 | High-resolution cytological localization of the XhoI and EcoRI repeat sequences in the pachytene ZW bivalent of the chicken. | The pachytene ZW pair of the chicken has been studied with a novel combination of fluorescent in situ hybridization and silver staining of the synaptonemal complexes, and with electron microscopic in situ hybridization. Probes for the EcoRI and the XhoI repeat sequences were used for light microscopy and probes for Xho... |
7749566 | Mapping of C-banded Crepis chromosomes by imaging methods. | Crepis capillaris is a highly suitable model plant for a computer-aided analysis of chromosomes because of its small number of chromosomes and well-defined bands. The identification of both large and small bands by means of profiles [grey value-function (position)] is frequently not possible with simple discrimination.... |
7749565 | Establishment of the marker order pter-NRAS-NGFB-D1S189-D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-qte r in relation to the centromere on human chromosome 1. | Recent developments in genetic linkage mapping of the human genome have generated a large number of short tandem repeat polymorphic markers (Weissenbach et al. 1992, Gyapay et al. 1994), and eventual integration of these markers into a physical map is a logical progression. A number of Généthon microsatellite (CA repea... |
7749564 | Whole-arm reciprocal translocation (WART) between Robertsonian chromosomes: finding of a Robertsonian heterozygous mouse with karyotype derived through WARTs. | The karyotype of a mouse trapped in a hybrid zone between a Robertsonian (Rb) population (2n = 22) and a population with the standard karyotype (2n = 40-alltelocentrics) shows two Rb chromosomes with new arm compositions. We suggest that whole-arm reciprocal translocations between Rb chromosomes gave rise to the new ch... |
7749563 | Efficient reidentification of chromosome slides from human metaphase spreads via microsatellite polymerase chain reaction. | In order to exclude inadvertent mixing of slides carrying metaphase spreads, chromosomal DNA was re-extracted several months after routine slide preparation (hypotonic treatment, spreading, fixation, staining, embedding and microscopic inspection) in different laboratories. The DNA yield was largely dependent on the nu... |
7749562 | Atomic force microscopy of plant chromosomes. | Atomic force microscopy has been used to image plant chromosomes from standard preparations without staining or coating. This has enabled the collection of high-resolution three-dimensional data on surface structure. The technique has been further applied to the imaging of C-banded chromosomes revealing structural chan... |
7749561 | Ordered mapping of three alpha satellite DNA subsets on human chromosome 22. | We report the physical order of three alphoid DNA subsets on human chromosome 22 determined by a combination of low- and high-resolution cytological mapping. Multicolor fluorescence in situ hybridization was performed on metaphase chromosomes, interphase nuclei and extended chromatin preparations. The results visually ... |
7749560 | Abnormal integrity of the nucleolus associated with cell cycle arrest owing to the temperature-sensitive ubiquitin-activating enzyme E1. | A mouse cell mutant, ts85, containing the temperature-sensitive ubiquitin-activating enzyme was arrested in G2 phase at the non-permissive temperature. In the arrested cells, azure C, a nucleolus-specific stain, revealed a U-shaped or ring-shaped arrangement of nucleolar lobes with an unstained region in the center. Si... |
7749559 | Characterization of Dasypyrum villosum (L.) candargy chromosomal chromatin by means of in situ restriction endonucleases, fluorochromes, silver staining and C-banding. | The distribution of C-banded heterochromatin was determined in an inbred line of Dasypyrum villosum. Practically no difference in chromosome morphology or band distribution could be observed within the chromosomes of the same pair. Heterochromatin bands, revealed by Giemsa banding, were characterized by means of their ... |
7538406 | Chromosome identification and mapping in the grass Zingeria biebersteiniana (2n = 4) using fluorochromes. | The grass Zingeria biebersteiniana is one of five angiosperms known with 2n = 2x = 4. Its chromosomes were studied using fluorochrome banding and fluorescence in situ hybridization (FISH). The large pericentromeric region fluoresced much more brightly on chromosome 2 than on chromosome 1, using two different fluorochro... |
7538061 | Topical FK-506 prevents experimental corneal allograft rejection. | We evaluated the efficacy of topical cyclodextrin-encapsulated FK-506 in the prevention of experimental corneal allograft rejection. Two weeks after inducing corneal inflammation and neovascularization with 8-0 silk sutures, 23 albino rabbits received a unilateral 8-mm diameter central penetrating corneal allograft fro... |
7736469 | Stereoselective thermal transfer of fructose from sucrose to cyclodextrins. | Branched cyclodextrins (CDs) have been formed by the thermal transfer of a fructosyl group from sucrose to O-6 of one of the glucose residues of cyclomaltohexa- and hepta-ose (alpha-CD and beta-CD). In each case the fructosyl group adds almost entirely in the beta configuration. The resultant fructosylcyclodextrins (Fr... |
7735460 | Capillary electrophoresis of carboxylated carbohydrates. I. Selective precolumn derivatization of gangliosides with UV absorbing and fluorescent tags. | We demonstrate that the precolumn derivatization reaction, recently introduced by our laboratory for the selective labeling of carboxylated monosaccharides, can be readily transposed to other glycoconjugates containing carboxylated sugar residues, namely sialogangliosides. The selective derivatization reaction describe... |
7731811 | S-adenosyl methionine alters the DNA contacts of the EcoKI methyltransferase. | The EcoKI methyltransferase methylates two adenines on opposite strands of its bipartite DNA recognition sequence AAC(N6)GTGC. The enzyme has a strong preference for hemimethylated DNA substrates, but the methylation state of the DNA does not influence its binding affinity. Methylation interference was used to compare ... |
7731800 | Transferring the purine 2-amino group from guanines to adenines in DNA changes the sequence-specific binding of antibiotics. | The proposition that the 2-amino group of guanine plays a critical role in determining how antibiotics recognise their binding sites in DNA has been tested by relocating it, using tyrT DNA derivative molecules substituted with inosine plus 2,6-diaminopurine (DAP). Irrespective of their mode of interaction with DNA, suc... |
7719454 | Extending the scope of chiral separation of basic compounds by cyclodextrin-mediated capillary zone electrophoresis. | In a previous paper on cyclodextrin-mediated capillary zone electrophoresis, it was shown that the use of short-chain tetraalkylammonium cations leads to a reversal in the direction of the electroosmotic flow without an adverse effect on enantioselectivity. As a result, enantiomeric resolution of basic (cationic) compo... |
7718114 | A preliminary exploration of the relationship between tumour necrosis factor (TNF) and monocytic in vitro production of interleukin-1 (IL-1) and internal organ dysfunction in severely burned patients. | Biological assays were adopted in this study to examine the changes in serum tumour necrosis factor (TNF) activity and blood monocytic in vitro production of interleukin 1 (IL-1) in 24 severely burned patients. The myocardial and hepatic enzymes (which included aspartate aminotransferase (AST), creatine kinase (CPK), l... |
7709228 | [A standardized large animal model of multiple organ failure after severe trauma]. | In the past various attempts have been made to develop a standardized animal model of multiple organ failure (MOF). Until now there has been no large animal model, that imitates the clinical situation of multiple trauma patients up to MOF. In a manner similar to the pathophysiological sequence in multiple trauma patien... |
7700965 | Penetration of hydrocortisone into excised human skin under the influence of cyclodextrins. | Hydrocortisone (HC) penetration into excised human skin from 1% (w/w) HC containing formulations with white petrolatum, an o/w cream and an aqueous polyacrylate gel is described. The experiments were performed with pure HC and the inclusion compounds with beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin. The small... |
7696515 | Grand canonical Monte Carlo molecular and thermodynamic predictions of ion effects on binding of an oligocation (L8+) to the center of DNA oligomers. | Grand canonical Monte Carlo (GCMC) simulations are reported for aqueous solutions containing excess univalent salt (activities a +/- = 1.76-12.3 mM) and one of the following species: an octacationic rod-like ligand, L8+; a B-DNA oligomer with N phosphate charges (8 < or = N < or = 100); or a complex resulting from the ... |
7696496 | Molecular dynamics simulations of the glucocorticoid receptor DNA-binding domain in complex with DNA and free in solution. | Molecular dynamics simulations have been performed on the glucocorticoid receptor DNA binding domain (GR DBD) in aqueous solution as a dimer in complex with DNA and as a free monomer. In the simulated complex, we find a slightly increased bending of the DNA helix axis compared with the crystal structure in the spacer r... |
7899500 | Sequence specificity of tetrahydrobenzopsoralen photobinding to DNA. | The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity was... |
7899091 | A new DNA nanostructure, the G-wire, imaged by scanning probe microscopy. | G-DNA is a polymorphic family of quadruple helical nucleic acid structures containing guanine tetrad motifs [G-quartets; Williamson, J.R., Raghuraman, M.K. and Cech, T.R. (1989) Cell 59, 871-880; Williamson, J.R. (1993) Proc. Natl. Acad. Sci. USA 90, 3124-3124]. Guanine rich oligonucleotides that are self-complimentary... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.