type
string
sequence
string
canonical_smiles
string
pKd_value
float64
label
int64
buffer
string
origin
string
source
string
RNA
CGAUCGGCGACUCACUCUCCAGUGAUUCGGGUAGAAACUGCGAA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
UAGGCGGCUGACUAUCACGUCAGGGAAGAAACUGCGCCUG
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
UGGUUGGUUGGCAAAACUGCGAAGGAAGACACUGCAUCCA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
ACUGGGGCUUUUGCUAAAGUCGGAAGAAAAUGCAGU
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
CGAACGGGGGAAGAAACUGCAAUCCGUUGGCCCGCUCG
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
CGGGGCCUAGGUGCCUAGGUCGGUAGAAACUGCGUUACGAUUAAA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GCACUUGGGGAAGAUACUGGGCCAAAACAUGGUCGGCCGAUUGC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GAAGUGGAAGAACCUGCAGCCCAUCGGCUGGACUU
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
CCGGGACAAUGUGGAGGACUCUGCGACGAAUUUAUCGGAUCC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
AAUCAGGAGGUAGAUACUGCAAUCGCGGUUGGUC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
CCAGGUGGAAGACCAUGCGCCUCGAGGCGGACCUAGG
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
CGCACACCCACGUGGAAGAACUGCAGCCAUCAGGCUGGACCUGUGUGUGCG
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
CAAGCCGUGGAAGACACUGCUCGAAUGUUCGAGGACGAC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GAUCAAGUGGAAGACGCUGCAUGGCCGUGGAC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
AGCGUGGAAGAAACUGCGAAUCAGCCCAAGACCG
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
AGCAUGGAAGAAACUGCGAGAUCGAUA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GUGUGGAAGAUCCUGCGCAUGGC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
CCGGUACAUGGAAGAACUGCGAA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GAGCAUGGUAGAAACUGCGCGUCA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GAGCAUGGAAGAACCUGCGACCAA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GAGCAUGGUAGAAACUGCGAAUCAAUCGA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
AGCAUGGAAGACACUGCGAAACAGGC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
UUGGAGCAUGGAAGACCCUGCGAAUCGAGAUUACAA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GGCAUGGUAGACACUGCGAAUCCGGGC
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
UGUUAGAGGGAAGAAACUGUAUCAGAGCGUU
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
AAAAUCGCAAUCAACCGUGGCUGGG
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GGGCAUGGUAGAAACUGCGGAUCGA
C[S+](CCC(N)C(=O)[O-])CC1OC(n2cnc3c(N)ncnc32)C(O)C1O
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9115371/
Aptamer Base
RNA
GGACGGCACCACGGUCGGAUCCGUGAGUUGUGACAAUUUAGCGGGUGGUAUUAGAGCCUACUGCCACAGCAAUAGGAUCGAUACAGAUCU
CCN(CC)c1ccc2c(-c3ccc(S(=O)(=O)O)cc3S(=O)(=O)[O-])c3ccc(=[N+](CC)CC)cc-3oc2c1
null
1
null
https://pubmed.ncbi.nlm.nih.gov/9889155/
Aptamer Base
DNA
GGGCACCGCACTCACACACCACATCAATCCAAGCTCCA
NC(=O)C(N)Cc1ccc(O)cc1
null
1
null
https://pubmed.ncbi.nlm.nih.gov/11557341/
Aptamer Base
DNA
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
NC(=O)C(N)Cc1ccc(O)cc1
null
1
null
https://pubmed.ncbi.nlm.nih.gov/11557341/
Aptamer Base
DNA
ATCTCTAGAGGATCCCCGGGTGGACACGGTGGCTTA
C=Cc1ccc(OC)cc1
7.042632
1
Not explicitly provided in the study
Hu, M., Wang, J., Liu, H., Chen, Q., Xie, C., Li, Q., Sun, J., Liao, D., Liu, Y., & Liu, H.-B. (2023). Development of functional nucleic acid sensor for detection of locust pheromone 4-vinylanisole. Chemical Engineering Journal, 466, 143065
AptaGen
DNA
ATCCAGAGTGACGCAGCAATGCTGCGTCACTCTGGATAATCTCTAGAGGATCCCCGGGTCCACACGGTGGCTTAGT
C=Cc1ccc(OC)cc1
6.787413
1
Not explicitly provided in the study
Hu, M., Wang, J., Liu, H., Chen, Q., Xie, C., Li, Q., Sun, J., Liao, D., Liu, Y., & Liu, H.-B. (2023). Development of functional nucleic acid sensor for detection of locust pheromone 4-vinylanisole. Chemical Engineering Journal, 466, 143065
AptaGen
DNA
ATCCAGAGTGACGCAGCAAGTCTCTGACACAATGTTCTTGTTCACTAATATTGAGATATGGACACGGTGGCTTAGT
C=Cc1ccc(OC)cc1
6.613769
1
Not explicitly provided in the study
Hu, M., Wang, J., Liu, H., Chen, Q., Xie, C., Li, Q., Sun, J., Liao, D., Liu, Y., & Liu, H.-B. (2023). Development of functional nucleic acid sensor for detection of locust pheromone 4-vinylanisole. Chemical Engineering Journal, 466, 143065
AptaGen
DNA
ATCCAGAGTGACGCAGCATATGCTATCTGTAGGCGACGTGCGTAGTTATGTTCAGTTTTGGACACGGTGGCTTAGT
C=Cc1ccc(OC)cc1
6.083657
1
Not explicitly provided in the study
Hu, M., Wang, J., Liu, H., Chen, Q., Xie, C., Li, Q., Sun, J., Liao, D., Liu, Y., & Liu, H.-B. (2023). Development of functional nucleic acid sensor for detection of locust pheromone 4-vinylanisole. Chemical Engineering Journal, 466, 143065
AptaGen
DNA
ATCCAGAGTGACGCAGCATACTTTGAATATTACGCAGCCAGCAAAAAGACTCTTAGATAATGTGGACACGGTGGCTTAGT
C=Cc1ccc(OC)cc1
6.171733
1
Not explicitly provided in the study
Hu, M., Wang, J., Liu, H., Chen, Q., Xie, C., Li, Q., Sun, J., Liao, D., Liu, Y., & Liu, H.-B. (2023). Development of functional nucleic acid sensor for detection of locust pheromone 4-vinylanisole. Chemical Engineering Journal, 466, 143065
AptaGen
DNA
TAGGGAATTCGTCGACGGATCCATGGCACATGGATACTAGCGAACGCATGGGCGGCCGCGGCGCATGCGTCGACCTG
CC12CC1(C)C(=O)N(c1cc(Cl)cc(Cl)c1)C2=O
7.615826
1
Buffer: 1×BB buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl, 1 mM MgCl₂, pH 7.4)
Dong, H., Wang, H., Guo, Z., Huang, J., Zhang, P., Guo, Y., & Sun, X. (2024). Combination of Capture-SELEX and Post-SELEX for procymidone-specific aptamer selection and broad-specificity aptamer discovery, and development of aptamer-based lateral flow assay. Analytica Chimica Acta, 1318, 342922. https://doi.org/10.1016...
AptaGen
DNA
GTAGCGGCGGTGTGGGCATTTTGGGCTAAA
O=Cc1ccc(CO)o1
5.055517
1
The 9th library of aptamers were incubated with the target for 1 gr at 37C, then an enzyme called T5 Exo was added to the mixture to digest unbound ssDNA library.
Liu, S., Cheng, J., Zhang, X., Qin, Y., Li, Y., Liu, H., Wu, Q., Hou, J., & Liu, X. (2025). T5 exonuclease improves the identification of aptamers specific for small molecules from the early screening library. Analytical Chemistry, 97(15), 8444–8451. https://doi.org/10.1021/acs.analchem.5c00251
AptaGen
DNA
GTATTGCGGAGGAAGGTTTTTAACCTTCGGGG
Nc1ncnc2c1ncn2C1OC(CO)C(O)C1O
7.829738
1
Column Buffer: 300 mM NaC1, 5 mM MgC12, 20 mM Tris (pH 7.6)
Li, Y., Liu, B., Huang, Z., & Liu, J. (2020). Engineering base-excised aptamers for highly specific recognition of adenosine. Chemical Science. doi:10.1039/d0sc00086h
AptaGen
DNA
TGCTAGACGATATTCGTCCATCCGAGCCCGTGGCGGGCTTTAGGACTCTGCGGCTTCGCGGCGCTGTCAGACTGAATATGTCA
O=C(O)CNCP(=O)(O)O
6.221849
1
qPCR detection assay from magnetic bead preparation in tap water / deionized water.
Shao Y, Tian R, Duan J, Wang M, Cao J, Cao Z, Li G, Jin F, Abd El-Aty AM, She Y. A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water. Sensors. 2023; 23(2):649. https://doi.org/10.3390/s23020649
AptaGen
DNA
TTCAAATCTAGCGCAGGTAGGGGTGTAGGTGTGGGGGTAAGTCTAGATTTGAA
CC(C)C(NC(=O)c1nn(Cc2ccc(F)cc2)c2ccccc12)C(N)=O
7.619789
1
null
Obtin Alkhamis, Caleb Byrd, Juan Canoura, Adara Bacon, Ransom Hill, Yi Xiao, Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity, Nucleic Acids Research, Volume 53, Issue 6, 11 April 2025, gkaf219, https://doi.org/10.1093/nar/gkaf219
AptaGen
DNA
ATCCGTCACACCTGCTCTCAGGGGATCACATTCTTGACGGTGTGATACAGTGCCTGGTGTTGGCTCCCGTAT
CC(=O)OCC[N+](C)(C)C
null
1
Binding Buffer: 0.5 M NaCl, 10 mM Tris HCl, and 1 mM MgCl
J.G. Bruno et al. Development of DNA aptamers for cytochemical detection of acetylcholine.
AptaGen
DNA
ACGACGTTTGCGATGAGAAACGTATGGTTTCGAAGGTCGT
Nc1ncnc2c1ncn2C1OC(CO)C(O)C1O
5.60206
1
SELEX buffer: 50 mM Tris, pH 7.6, 500 mM NaCl, 20 mM MgCl2
null
AptaGen
DNA
ACGACACGGAGGCTTAGTTTGCTAAATGGTCATGTCGT
Nc1ncnc2c1ncn2C1OC(CO)C(O)C1O
5.638272
1
SELEX buffer: 50 mM Tris, pH 7.6, 500 mM NaCl, 20 mM MgCl2
null
AptaGen
RNA
GGGAGAGGAUACUACACGUGAUAGGACGAUUAUCGAAAAUCACCAGAUUGGACCCUGGUUAACGAUCCAUUGCAUGUAGCAGAAGCUUCCG
Nc1ncnc2nc[nH]c12
5
1
Binding Buffer: 100 mM HEPES, 500 mM NaCl, 5 mM MgCl
Meli, M.,
AptaGen
DNA
CCTGGGGGAGTATTGCGGAGGAAGG
Nc1ncnc2c1ncn2C1OC(CO)C(O)C1O
5.221849
1
Column Buffer: 300 mM NaC1, 5 mM MgC1
Huizenga and Szostak. "A DNA Aptamer That Binds Adenosine and ATP." Biochemistry, 34 (1995): 656-665.
AptaGen
RNA
GGGAGAUCUACGGAUCUCAGGGCUCUUACGGGAGCUACAUGGAAGGAGUCCAUGUGU
Nc1ncnc2c1ncn2C1OC(COP(=O)(O)OP(=O)(O)OP(=O)(O)O)C(O)C1O
5.318759
1
300 mM NaCl, 10 mM MgCl
P Sazani, R Larralde, and J Szostak.(2004) "A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP." J Am Chem Soc.
AptaGen
RNA
GGGCGACCCUGAUGAGCACACGAGGGGGAAACCCCGGACAAUCAGACACGGUGUUCGAAACGGUGAAAGCCGUAGGUUGCCCUUU
Nc1ncnc2c1ncn2C1OC(COP(=O)(O)OP(=O)(O)O)C(O)C1O
null
1
Selection Buffer ((50 mM HEPES [pH 7.4], 25 mM MgCl2, 150 mM NaCl) containing 10 g/ml tRNA)
Srinivasan et al. "ADP-Specific Sensors Enable Universal Assay of Protein Kinase Activity." Chemistry & Biology, 11(2004): 499-508.
AptaGen
DNA
AGCAGCACAGAGGTCAGATGGTGCTATCATGCGCTCAATGGGAGACTTTAGCTGCCCCCACCTATGCGTGCTACCGTGAA
COc1cc2c(c3oc(=O)c4c(c13)CCC4=O)C1C=COC1O2
7.943476
1
pH 7.0: 100 mmol/L NaCl, 20 mmol/L Trisƒ??HCl pH 7.6, 2 mmol/L MgCl2, 5 mmol/L KCl, 1 mmol/L CaCl2, 0.02 % Tween 20
Ma, X., Wang, W., Chen, X., Xia, Y., Wu, S., Duan, N., and Wang, Z. "Selection, identification, and application of Aflatoxin B1 aptamer." European Food Research and Technology 238.6 (2014): 919-925.
AptaGen
DNA
ATCCGTCACACCTGCTCTGACGCTGGGGTCGACCCGGAGAAATCGCATTCCCCTGTGGTGTTGGCTCCCGTAT
COc1cc2c(c3oc(=O)c4c(c13)CCC4=O)C1(O)C=COC1O2
7.44855
1
20 mM Tris-HCL, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2
Malhotra, S., Pandey, A., Rajput, S. and Sharma, R. "Selection of aptamers for Aflatoxin M1 and their characterization." Journal of Molecular Recognition 27 (2014): 493-500.
AptaGen
DNA
CTCGTTATGACTAACGCTAGCCCGGGG
NN1CC(=O)NC1=O
7.065502
1
Binding buffer: pH 7.4. 100mM NaCl, 2mM MgCl2, 20mM Tris-HCl, 1 mM CaCl2, 5mM KCl. 25°C in the dark for 2 hours.
Zheng, X., Jiang, S., Ren, Y., Wang, S., Xie, Y., & Le, T. (2024). High-efficient selection of aptamers by magnetic cross-linking precipitation and development of aptasensor for 1-aminohydantoin detection. LWT - Food Science and Technology, 199. https://doi.org/10.1016/j.lwt.2024.116128
AptaGen
DNA
GCGGGCGGTTGTATAGCGG
CC1(C)SC2C(NC(=O)C(N)c3ccccc3)C(=O)N2C1C(=O)O
7.872895
1
Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl
Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods.
AptaGen
DNA
TTTAGTTGGGGTTCAGTTG
CC1(C)SC2C(NC(=O)C(N)c3ccccc3)C(=O)N2C1C(=O)O
8.008774
1
Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl
Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods.
AptaGen
DNA
CACGGCATGGTGGGCGTCGTG
CC1(C)SC2C(NC(=O)C(N)c3ccccc3)C(=O)N2C1C(=O)O
8.026872
1
Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl
Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods.
AptaGen
DNA
CTGTTTGGTAGGCAG
Cc1ncc([N+](=O)[O-])n1CCO
7.948076
1
200mL binding buffer: 50 mM Tris-HCl containing 5 mM KCl, 100 mM NaCl and 1 mMMgCl2, pH 7.4
Wei, Hao et al. “Screening and application of a truncated aptamer for high-sensitive fluorescent detection of metronidazole.” Analytica chimica acta vol. 1128 (2020): 203-210. doi:10.1016/j.aca.2020.07.003
AptaGen
RNA
GGGAGAAGGCGGCGCGUAGGCGAGCUUUAGGCCCGACAUUCCCCUAAAAAAGCUUGUUCUAUUCGUGGACGAUGCGCAC
CNC1C(OC2OC(CO)C(N)C(O)C2O)OC2CC(N)C(OC3C(N)CC(N)C(O)C3O)OC2C1O
7.546682
1
Binding Buffer: PBS with 10% (v/v) methanol
N. Derbyshire et al. Toggled RNA aptamers against aminoglycosides allowing facile detection of antibiotics using gold nanoparticle assays.
AptaGen
DNA
CTTACGACTCAGGCATTTTGCCGGGTAACGAAGTTACTGTCGTAAG
CCCC(C(=O)c1ccc2c(c1)OCO2)N1CCCC1
8.154902
1
10 mM Tris–HCl, 0.5 mM MgCl2, 20 mM NaCl, pH 7.4
Haixiang Yu, Weijuan Yang, Obtin Alkhamis, Juan Canoura, Kyung-Ae Yang, Yi Xiao, In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality, Nucleic Acids Research, Volume 46, Issue 8, 4 May 2018, Page e43, https://doi.org/10.1093/nar/gky026
AptaGen
DNA
GTGGAGGCGGTGGCCAGTCTCGCGGTGGCGGC
O=C1OC(C(O)CO)C(O)=C1O
6.005287
1
50mM sodium acetate, 1% (w/v) BSA, 0.05% (v/v) Tween 20, 1mM MgCl2 (Sigma, all components) adjusted to pH 5.5
Chiu, A.S., Sankarapani, V., Drabek, R., Jackson, G.W., Batchelor, R.H. and Kim, Y., 2018. Inhibition of vitamin C oxidation by DNA aptamers. Aptamers, 2, pp.1-20.
AptaGen
DNA
GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATTTTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCGAGATAGTAAGTGCAATCT
[As]
8.305395
1
Selection buffer: 150 mM NaCl, 50mM HEPES, 0.5 mM MgCl
Kim,
AptaGen
RNA
GGGUUGGGAAGAAACUGUGGCACUUCGGUGCCAGCAACCC
Nc1ncnc2c1ncn2C1OC(COP(=O)(O)OP(=O)(O)OP(=O)(O)O)C(O)C1O
6.154902
1
Binding Buffer (300mM NaCl, 20 mM Tris (pH 7.6), 5mM MgCl2)
sassanfar and Szostak. "An RNA motif that binds ATP."
AptaGen
RNA
GGGCGACCCUGAUGAGCCUUGGGAAGAAACUGUGGCACUUCGGUGCCAGCACGUCGAAACGGUGAAAGCCGUAGGUUGCCC
Nc1ncnc2c1ncn2C1OC(COP(=O)(O)OP(=O)(O)OP(=O)(O)O)C(O)C1O
null
1
reaction buffer [50 mM Tris.HCl (pH 7.5) and 20 mM MgCl2] for 20 hr
Garrett A. Soukup and Ronald R. Breaker. "Engineering precision RNA molecular switches"
AptaGen
RNA
GGGCGACCCUGAUGAGAUGGGGAAGAAACUGUGGCACUUCGGUGCCAGCCUCUCGAAACGGUGAAAGCCGUAGGUUGCCC
Nc1ncnc2c1ncn2C1OC(COP(=O)(O)OP(=O)(O)OP(=O)(O)O)C(O)C1O
null
1
Reaction buffer [50 mM Tris.HCl (pH 7.5) and 20 mM MgCl2] for 20 hr
Garrett A. Soukup and Ronald R. Breaker. "Engineering precision RNA molecular switches"
AptaGen
DNA
TGTACCGTCTGAGCGATTCGTACGAACGGCTTTGTACTGTTTGCACTGGCGGATTTAGCCAGTCAGTGTTAAGGAGTGC
CCNc1nc(Cl)nc(NC(C)C)n1
9.207608
1
500 µL of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride
Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332
AptaGen
DNA
TGTACCGTCTGAGCGATTCGTACCATTAGTGGGTGCTCCTTACCTGATGGTCATCTAGCCAGTCAGTGTTAAGGAGTGC
CCNc1nc(Cl)nc(NC(C)C)n1
8.29243
1
500 µL of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride
Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332
AptaGen
DNA
CACTGGATAGTCAACTCTGGCAACGAAGTGCTCCTCCAAGGTTAGGTGACGGTATTGACTGGATCGCAATCATTTGCTACTGACTATGGAAGCTC
C1=Cc2ccccc2NN=C1
null
1
Annealing Buffer: 50 mM Tris pH 8.0 150 mM NaCl
Samuelian, John S., and Dana A. Baum. “Identification of DNA aptamers for benzodiazepine detection in synthetic urine.” Analysis & Sensing, vol. 2, no. 6, 10 Aug. 2022, https://doi.org/10.1002/anse.202200044.
AptaGen
DNA
GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC
CC12CCC3c4ccc(O)cc4CCC3C1CCC2O
5.221849
1
1X Binding Buffer: 20 mm Tris (pH 7.4), 1 M NaCl, 10 mM MgCl
Yang K, Pei R, Stefanovic D, Stojanovic M.N. (2011) Optimizing Cross-reactivity with Evolutionary Search for Sensors.
AptaGen
DNA
ATAAGCTCTTAGCGCGTGTTTATCCACCTTT
C=CC1=C(C)C(=Cc2[nH]c(Cc3[nH]c(C=C4NC(=O)C(C)=C4C=C)c(C)c3CCC(=O)O)c(CCC(=O)O)c2C)NC1=O
6.692504
1
Selection buffer - 200 mM NaCl, 10 mM phosphate, 2 mM MgCl2, pH 7.6. The randomized aptamer library was annealed with the biotinylated capture strand in selection buffer. Streptavidin-agarose resin was loaded, and biotin-ssDNA complex was added into it. Bilirubin was loaded onto the library and the eluted ssDNA was obt...
Yachen Xie, Yunus A. Kaiyum, Lide Gu, Yibo Liu, Philip E. Johnson, and Juewen Liu
AptaGen
DNA
GGGTGTGGAACACGAACGTAGTGCGAATACTTTCGA
C=CC1=C(C)C(C=c2[nH]c(=Cc3[nH]c(C=C4NC(=O)C(C)=C4C=C)c(C)c3CCC(=O)O)c(CCC(=O)O)c2C)=NC1=O
8.19382
1
Selection buffer - 200 mM NaCl, 10 mM phosphate, 2 mM MgCl2, pH 7.6. The randomized aptamer library was annealed with the biotinylated capture strand in selection buffer. Streptavidin-agarose resin was loaded, and biotin-ssDNA complex was added into it. Bilirubin was loaded onto the library and the eluted ssDNA was obt...
Yachen Xie, Yunus A. Kaiyum, Lide Gu, Yibo Liu, Philip E. Johnson, and Juewen Liu
AptaGen
DNA
ATCGACTGACCCATATGGATCCCCTATTCGTCGGGACATTATCTCTCGTGTAAGCTTGAT
COc1cc2c(c3oc(=O)c4c(c13)CCC4=O)C1C=COC1O2
5.065502
1
PBSMT: (pH 7.2, 137 mM NaCl, 2.68 mM KCl, 8.1 mM Na2HPO4, 1.76 mM KH2PO4, 1 mM MgCl2, 0.025% Tween 20) and 20% human serum.
Yao L, Feng J, Zhou Y, Gao S,
AptaGen
RNA
GGAAGAGAUGGCGACUAAAACGACUUGUCGC
Nc1ncnc2c1ncn2C1OC2COP(=O)(O)OC2C1O
5
1
Binding Buffer (20 mM Tris-HCl (pH 7.5 at 23 °C), 450 mM NaCl, 100 mM KCl, 10 mM MgCl
Kozumi and Breaker. "Molecular Recognition of cAMP by an RNA Aptamer." Biochemistry, 39 (2000): 8983-8992.
AptaGen
DNA
CTCCTTCAACGAAGTGGGTCTCCTTCAACGAAGTGGGTCTC
COC(=O)C1C(OC(=O)c2ccccc2)CC2CCC1N2C
7.443697
1
10% saliva in 10 mM Tris, 0.1 mM MgCl2, pH 7.4
Yu, H., Canoura, J., Guntupalli, B., Lou, X., & Xiao, Y. (2017). A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva. Chemical Science, 8(1), 131–141. doi:10.1039/c6sc01833e
AptaGen
DNA
GAGACAAGGGACAAGGAG
COC(=O)C1C(OC(=O)c2ccccc2)CC2CCC1N2C
7.443697
1
10% saliva in 10 mM Tris, 0.1 mM MgCl2, pH 7.4
Yu, H., Canoura, J., Guntupalli, B., Lou, X., & Xiao, Y. (2017). A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva. Chemical Science, 8(1), 131–141. doi:10.1039/c6sc01833e
AptaGen
RNA
GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGAAAGCCAAAGCCGUAGCGCAGAUGAUCUCGCCAUCAGUACCGAAACGGUAGCGAGAGCUC
[Cd+2]
4
0
Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl
Seetharaman et al. "Immobolized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341.
AptaGen
DNA
GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG
OCC1OC(OC2C(CO)OC(O)C(O)C2O)C(O)C(O)C1O
6.221849
1
Binding buffer: 20 mM Tris (pH 7.5), 100 mM NaCl, 5 mM MgCl
Yang, Q.,
AptaGen
DNA
AGCAGCACAGAGGTCAGATGACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAGCCTATGCGTGCTACCGTGAA
O=C(NC(CO)C(O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
6.115771
1
100mM NaCl, 20 mM Tris-HCl, 2 mM MgCl
J Mehta
AptaGen
RNA
GGGAUCACAGUGAAAAAAGACGUGUGAAUGUCACACUGAAAAAAGAUCCC
O=C(NC(CO)C(O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
6.958607
1
Binding Buffer (20 mM MgCl2, 400 mM NaCl, 100 mM bis-Tris (pH 6.4)
Burke et al. "RNA aptamers to the peptidyl transferase inhibitor chloramphenicol." Chemistry & Biology, 4(1997): 833-843.
AptaGen
DNA
GTACCAGCTTATTCAATTACCGCGAAGAAGTGTCATTGTTTTGGAGATTCGAAGCGCTGTACACAGGTAATGAAGCCTTCTAAGATAGTATGTTCATCAG
CC(CCC(=O)O)C1CCC2C3C(O)CC4CC(O)CCC4(C)C3CC(O)C12C
10.30103
1
selection buffer (50 mM Trisƒ??HCl, 300 mM NaCl, 30 mM KCl, 5 mM MgCl2, pH 7.6
Kato, T., et al. "In vitro selection of DNA aptamers which bind to cholic acid." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1493 (2000):
AptaGen
DNA
ACGACGGTGGCATGAACATAAGTTCCCCCTTGAAAGTCGT
CC12CCC3c4ccc(O)cc4CCC3C1CCC2O
7.60206
1
SELEX Buffer: 20 mM Tris, pH 7.4, 500 mM NaCl, 10 mM MgCl2.
Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively Chenqi Niu, Chong Zhang, and Juewen Liu Environmental Science & Technology 2022 56 (24), 17702-17711 DOI: 10.1021/acs.est.2c05808
AptaGen
DNA
CGACTTAAGGTATGTGATCTTAGTTGTAGTTCAAGTCG
CC12CCC3c4ccc(O)cc4CCC3C1CCC2O
7.853872
1
SELEX Buffer: 20 mM Tris, pH 7.4, 500 mM NaCl, 10 mM MgCl2.
Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively
AptaGen
RNA
GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGAAAGCCAAAGCCGUAGCGCAGAUGAUCUCGCCAUCAGUACCGAAACGGUAGCGAGAGCUC
[Co+2]
4
0
Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl
Seetharaman et al. "Immobolized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341.
AptaGen
DNA
GACAAGGATAAATCCTTCAATGAAGTGGGTCACTCATCTGTGA
COC(=O)C1C(OC(=O)c2ccccc2)CC2CCC1N2C
null
1
The buffer for immobilization of ssDNA probe consisted of 0.8M phosphate buffer (PB) with 1.0M NaCl, 5 mM MgCl2 and 1 mM ethylenediamine tetraacetic acid (EDTA), pH 7.0. The DNA hybridization buffer and cocaine incubation buffer was composed of 50mM PB with 100mM K2SO4 (pH 7.4).
Yang, Z. et al. Community Sewage Sensors towards Evaluation of Drug Use Trends: Detection of Cocaine in Wastewater with DNA-Directed Immobilization Aptamer Sensors. Sci. Rep. 6, 21024; doi: 10.1038/srep21024 (2016).
AptaGen
DNA
GGGAGACAAGGAAAATCCTTCAATGAAGTGGGTCGACA
COC(=O)C1C(OC(=O)c2ccccc2)CC2CCC1N2C
6.39794
1
Phosphate buffer: 20 mM, pH7, 200 mM NaCl.
M Stojanovic, P de Prada, and D Landry. Aptamer-based folding fluorescent sensor for cocaine.
AptaGen
DNA
GAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT
CC12CCC(=O)C=C1CCC1C2C(O)CC2(C)C1CCC2(O)C(=O)CO
4.793174
1
50 mM Tris, 137 mM NaCl, 5 mM MgCl2, pH 7.4.
Martin, J. A., Chávez, J. L., Chushak, Y., Chapleau, R. R., Hagen, J., & Kelley-Loughnane, N. (2014). Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol. Anal. Bioanal. Chem. 2015 Mar 13; 406(19): 4637–4647. doi:10.1007/s00216-014-7883-8
AptaGen
DNA
GACGACGCCCGCATGTTCCATGGATAGTCTTGACTAGTCGTC
CC12CCC(=O)C=C1CCC1C2C(O)CC2(C)C1CCC2(O)C(=O)CO
6.610834
1
To study aptamer binding under different conditions, a number of buffers were prepared. Buffer 1 (the buffer used for selecting the CSS.1 aptamer): 20 mM HEPES (pH 7.5), 5 mM KCl, 1 M NaCl and 10 mM MgCl2. Buffer 1-1: 20 mM HEPES (pH 7.5), 5 mM KCl, 100 mM NaCl and 10 mM MgCl2. Buffer 1-2: 20 mM HEPES (pH 7.5), 5 mM KC...
Niu, C., Ding, Y., Zhang, C., & Liu, J. (2022). Comparing two cortisol aptamers for label-free fluorescent and colorimetric biosensors. Sensors & Diagnostics, 1(3), 541–549. https://doi.org/10.1039/d2sd00042c
AptaGen
RNA
GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUGUGGAAACAGACGUGGCACAUGACUACGUCGAAACGGUAGCGAGAGCUC
Nc1ncnc2c1ncn2C1OC2COP(=O)(O)OC2C1O
null
1
Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl
Seetharaman et al. "Immbolized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341.
AptaGen
RNA
GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUUAGGGCCAAGUGUGGUGAAAGACACACUCGAAACGGUAGCGAGAGCUC
Nc1ccn(C2OC3COP(=O)(O)OC3C2O)c(=O)n1
null
1
Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl
Seetharaman et al. "Immobolized RNA switches for the analysis of complex chemical and biological mixture." Nature Biotechnology, 19(2001):336-341.
AptaGen
DNA
ATACCAGCTTATTCAATTTAATGCGGGGTGAGGCTCAATCAAGGAAAGATATAAGTAAGCAAAAAGGTCAAACAAGGGCGAGATAGTAAGTGCAATCT
NC1=NC(O)C2C3OC4(O)OC(C(O)C2(N1)C4O)C3(O)CO
9
1
1x PBS buffer with 1.5 mM MgCl2, 22°C
Shkembi et al., 2021
AptaGen
DNA
ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTACGTCT
COc1cccc2c1C(=O)c1c(O)c3c(c(O)c1C2=O)CC(O)(C(C)=O)CC3OC1CC(N)C(O)C(C)O1
6.565431
1
Binding Buffer (20 mM Tris-HCl (pH 7.4), 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, and 0.05% v/v Tween-20)
Wochner et al. "A DNA aptamer with high affinity and specificity for therapeutic anthracyclines." Analytical Biochemistry, 373(2008): 34-42.
AptaGen
DNA
GCATCACTACAGTCATTACGCATCGTAGGGGGGATCGTTAAGGAAGTGCCCGGAGGCGGTATCGTGTGAAGTGCTGTCCC
CC1=CC2OC3C(O)CC(C)(C34CO4)C2(CO)C(O)C1=O
6.158203
1
null
Wu, S. Q., Liu, H. X., & Liu, Y. W. (2011). Deoxynivalenol nucleic acid aptamer and
AptaGen
DNA
GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC
NCCc1ccc(O)c(O)c1
6.154902
1
Buffer: 50 mM Tris-HCl (pH 7.4), 5 mM MgCl
R Walsh and M DeRosa. Retention of function in the DNA homolog of the RNA dopamine aptamer.
AptaGen
RNA
GUCUCUGUGUGCGCCAGAGAACACUGGGGCAGAUAUGGGCCAGCACAGAAUGAGGCCC
NCCc1ccc(O)c(O)c1
null
1
Column Buffer: 50 mM Tris-HCl (pH 7.4), 5 mM MgCl
Mannironi, C., et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-9734.
AptaGen
RNA
GGGAAUUCCGCGUGUGCGCCGCGGAAGAGGGAAUAUAGAGGCCAGCACAUAGUGAGGCCCUCCUCCC
NCCc1ccc(O)c(O)c1
null
1
Assay Buffer: 10 mM PBS, pH 7.2, 5mM MgCl
Sequence from C Mannironi, et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-973.
AptaGen
DNA
GGGCGAGAACAGTGTCCCAGTCGCTAGTTTTCTCTGCCC
CC1c2cccc(O)c2C(O)=C2C(=O)C3(O)C(O)=C(C(N)=O)C(=O)C(N(C)C)C3C(O)C21
6.823909
1
20 mM Tris at pH 7.5 with 100 mM NaCl, 2 mM MgCl2, 5 mM KCl and 1 mM CaCl2, RT
Lee, K.H., Zeng, H. A general double library SELEX strategy for aptamer selection using unmodified nonimmobilized targets. Anal Bioanal Chem 409, 5081–5089 (2017).
AptaGen
DNA
TTCTGAATTAAAGAGAATAAATGAATTGAA
CCN1CCN(c2cc3c(cc2F)c(=O)c(C(=O)O)cn3C2CC2)CC1
6.742321
1
In SELEX Buffer (see Refolding Protocol) at 25°C with shaking at 200 rpm.
Enhanced Detection of Enrofloxacin in Seawater Using a Newly Selected Aptamer on a Graphite Oxide-Based Biosensor
AptaGen
DNA
GGACTTAAGGTATGTGATCTTAGTTGTAGTTCAAGTCCTTAAG
CC12CCC3c4ccc(O)cc4CCC3C1CCC2O
6.221849
1
20 mM HEPES pH 7.4,
Shi, L., Jin, Y., & Liu, J. (2024). Intramolecular aptamer switches. The Analyst, 149(3), 745–750. https://doi.org/10.1039/d3an02022c
AptaGen
DNA
ATACCAGCTTATTCAATTTGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTCAGATAGTAAGTGCAATCT
NCCO
8.221849
1
Binding Buffer (100 mM NaCl, 20 mM Tris/HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20).
Mann, Doerthe, et al. "In vitro selection of DNA aptamers binding ethanolamine." Biochemical and Biophysical Research Communication, 338 (2005): 1928-1934. Tradmarked in patent, "New DNA aptamers specific for compunds that contain the ethylamino group, useful for diagnosis, prevention and treatment of ethanolamino...
AptaGen
RNA
GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGCAUGAUGCAGAAGGACGUCGAAACGGUAGCGAGAGCUC
Cc1cc2nc3c(=O)[nH]c(=O)nc-3n(CC(O)C(O)C(O)COP(=O)(O)O)c2cc1C
null
1
Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl
Seetharaman et al. "Immobilized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341.
AptaGen
RNA
GGGCGACCCUGAUGAGCCUUAGGAUAUGCUUCGGCAGAAGGACGUCGAAACGGUGAAAGCCGUAGGUUGCCC
Cc1cc2nc3c(=O)[nH]c(=O)nc-3n(CC(O)C(O)C(O)COP(=O)(O)O)c2cc1C
null
1
Reaction buffer [50 mM Tris.HCl (pH 7.5) and 20 mM MgCl2] for 20 hr
Garrett A. Soukup and Ronald R. Breaker. "Engineering precision RNA molecular switches" Proc. Natl. Acad. Sci. 96(1999):3584-3589.
AptaGen
RNA
GGGCGACCCUGAUGAGAUGAGGAUAUGCUUCGGCAGAAGGCUCUCGAAACGGUGAAAGCCGUAGGUUGCCC
Cc1cc2nc3c(=O)[nH]c(=O)nc-3n(CC(O)C(O)C(O)COP(=O)(O)O)c2cc1C
null
1
Reaction buffer [50 mM Tris.HCl (pH 7.5) and 20 mM MgCl2] for 20 hr
Garrett A. Soukup and Ronald R. Breaker. "Engineering precision RNA molecular switches"
AptaGen
DNA
CTCTCGGGACGACGGCTGGCACGTTTGGTTCAAGAATGTGGGTGTCGTCCC
OCC1(O)OCC(O)C(O)C1O
8.39794
1
Shinkai's receptor 50uM with 2% methanol.
Yang KA, Barbu M, Halim M, et al. Recognition and sensing of low-epitope targets via ternary complexes with oligonucleotides and synthetic receptors. Nat Chem. 2014;6(11):1003-1008. doi:10.1038/nchem.2058
AptaGen
DNA
GGGACGACGGAGGTGTAGGGTTTAGGTAGTGGTTTGAAGTCGTCCC
Cc1c(Nc2ncccc2C(=O)O)cccc1C(F)(F)F
6.787812
1
null
Obtin Alkhamis, Caleb Byrd, Juan Canoura, Adara Bacon, Ransom Hill, Yi Xiao, Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity, Nucleic Acids Research, Volume 53, Issue 6, 11 April 2025, gkaf219, https://doi.org/10.1093/nar/gkaf219
AptaGen