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10,010,003
1. Four pairs of Scottish Blackface ewes were given a basal diet of hay, providing 8 mg zinc/d, and one of four levels of supplementary Zn (0,75, 150 or 225 mg/d) continuously by intraruminal infusion. 2. 65Zn (59 microCi) was given intravenously after stabilization for 14 d and the changes in specific radioactivity (S...
[ 0.028855806216597557, 0.02279965579509735, 0.03580235317349434, 0.08488728106021881, 0.029482321813702583, -0.03494708985090256, 0.012910251505672932, 0.08860383182764053, -0.03272247686982155, 0.033617474138736725, 0.04673922061920166, -0.029916470870375633, 0.010262309573590755, 0.102515...
10,010,004
Obligatory urinary and faecal nitrogen losses in young Chilean men given two levels of dietary energy intake.
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10,010,005
1. The obligatory nitrogen losses were measured in young adult males of the low socio-economic group, consuming an N-free diet at 192 kJ (46 kcal)/kg per d from day 1 to 10 and 243 kJ (58 kcal)/kg per d from day 11 to 18. 2. All subjects, except one, lost weight compatible with N loss. 3. A kinetic evaluation of the re...
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10,010,006
The availability to the chick of pantothenic acid in foods.
[ 0.028286438435316086, -0.062480486929416656, -0.0039937179535627365, 0.03298051655292511, -0.00183896254748106, 0.10996823012828827, 0.03947976604104042, 0.024021780118346214, 0.06989412009716034, -0.04237104579806328, -0.05681806802749634, -0.030375447124242783, 0.002675761468708515, -0.0...
10,010,007
1. The content of total pantothenic acid (PaA) in two foodstuffs of microbial origin was measured by standard microbiological and chick biological assays. 2. Substantially and consistently higher values were found by the chick assay than by the microbiological determination, indicating that PaA-containing compounds wer...
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10,010,008
The origin of urinary aromatic compounds excreted by ruminants. 1. The metabolism of quinic, cyclohexanecarboxylic and non-phenolic aromatic acids to benzoic acid.
[ -0.016001442447304726, -0.03590758889913559, -0.006720236502587795, 0.005487128160893917, -0.01785288378596306, -0.016119590029120445, 0.07421590387821198, 0.025003859773278236, 0.07984083145856857, -0.01832401566207409, 0.06268055737018585, -0.030150175094604492, -0.10696321725845337, 0.0...
10,010,009
1. The contribution of dietary constituents to the large urinary output of benzoic acid characteristic of ruminants and some herbivores is not well understood. 2. Methods for the analysis of quinic, cyclohexanecarboxylic, benzoic, phenylacetic, 3-phenylpropionic and cinnamic acids in urine and in rumen fluids were deve...
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10,010,010
The origin of urinary aromatic compounds excreted by ruminants. 2. The metabolism of phenolic cinnamic acids to benzoic acid.
[ -0.00683229323476553, -0.054157909005880356, -0.021984556689858437, 0.008399983868002892, -0.011345924809575081, 0.030404934659600258, 0.03262076899409294, 0.033271290361881256, 0.09265713393688202, -0.03662215918302536, 0.05279622972011566, -0.048941802233457565, -0.10963164269924164, 0.0...
10,010,011
1. The extent to which phenolic derivatives of benzoic acid (seven); of phenylacetic acid (one); of 3-phenylpropionic acid (one) and of cinnamic acid (six) served as precursors of the urinary benzoic acid excreted by sheep was determined after administration as continuous drips via rumen or abomasal cannulas. 2. Phenol...
[ 0.005433260928839445, -0.09037315100431442, -0.01936079002916813, -0.0072890883311629295, -0.019600311294198036, 0.05801735445857048, -0.024918226525187492, 0.059442583471536636, 0.0707089751958847, -0.04484514892101288, 0.047530513256788254, -0.036280207335948944, -0.04819154366850853, 0....
10,010,012
Effect of dietary vicine and vitamin E supplementation on the productive performance of growing and laying chickens.
[ 0.038514651358127594, -0.03536944091320038, -0.0030508546624332666, 0.002416743664070964, 0.05090373754501343, 0.10558720678091049, 0.000918553036171943, 0.020831894129514694, 0.057665351778268814, -0.00881860964000225, -0.01896626129746437, 0.011531572788953781, -0.038578856736421585, 0.0...
10,010,013
1. Experiments were conducted to study the effects of dietary vicine (2, 6-diamino-4, 5 dihydroxy pyrimidine-5 (beta-D-glucopyranoside)) and supplemental vitamin E on the performance of laying hens and growing chicks, haemolysis of erythrocytes than birds fed on a control diet. 3. Vicine when fed to laying hens had a v...
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10,010,014
The effect of dietary methyl branched-chain fatty acids on aspects of hepatic lipid metabolism in the rat.
[ 0.051503121852874756, -0.03292951360344887, -0.012329880148172379, 0.014213749207556248, -0.04759300500154495, 0.052326906472444534, -0.07019773125648499, -0.02162851020693779, -0.03779899701476097, -0.05835425481200218, -0.012650756165385246, -0.004106916952878237, -0.02986558899283409, -...
10,010,015
1. Rats were fed to appetite on a stock laboratory diet or on diets consisting of the stock diet and in addition 50 or 200 g triolein/kg, 50 g palmitic acid/kg or 50 g/kg of a concentrate mixture of methyl branched-chain fatty acids (Me-BCFA) which had been prepared from sheep adipose triacylglycerols. 2. No difference...
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10,010,016
Motility of the reticulum and rumen of sheep given juice-extracted pasture.
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10,010,017
1. Sheep were fed on different diets of juice-extracted herbage to determine what effect juice-extraction had on reticulo-rumen motility. 2. The frequency of A and B sequences of contraction of the reticulo-rumen were recorded during eating, rumination and inactivity for continuous periods of 24-72 h by using integrate...
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10,010,018
Fluorometric studies on the binding of gluconolactone, glucose, and glucosides to the subsites of glucoamylase.
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10,010,019
Stopped-flow kinetic studies on the binding of gluconolactone and maltose to glucoamylase.
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10,010,020
Functional properties of human adult hemoglobin specifically modified at the alpha-amino groups of the beta chains with D-glucose 6-phosphate.
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10,010,021
Rapid analysis of estrogen and progesterone receptors using gel-exclusion high-performance liquid chromatography.
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10,010,022
Estrogen and progesterone receptors prepared from mouse, rat, and human uteri, as well as from human breast cancers, have been characterized by gel-exclusion high-performance liquid chromatography. The qualitative relationships previously established by sedimentation analysis between the cytoplasmic [aggregated (approx...
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10,010,023
Determination of the free-energy coupling for binding of calcium ions and troponin I to calmodulin.
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10,010,024
Regulation of a wide variety of biological systems by Ca2+ is now known to be mediated through calmodulin, a Ca2+-binding protein. Calmodulin forms Ca2+-dependent complexes with several proteins, including troponin I. We have determined the free-energy coupling (delta GoCT) for binding of Ca2+ and troponin I to calmodu...
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10,010,025
Biosynthesis of cell surface sulfated glycoproteins by cultured vascular endothelial cells.
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10,010,026
Cultured vascular endothelial cells incorporate 35SO4 into a class of oligosaccharides which are N-glycosidically linked to cell-surface or extracellular proteins. This type of sulfated oligosaccharide was not synthesized by smooth muscle cell cultures and may represent sulfation of vascular tissue glycoproteins which ...
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10,010,027
Characterization of tubulin modified with the sulfhydryl-reactive fluorochrome monobromo(trimethylammonio)bimane.
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10,010,028
5-(dimethylamino)naphthalene-1-sulfonic acid, a fluorescent probe of the medium chain fatty acid binding site of serum albumin.
[ 0.007068211678415537, -0.02706139162182808, -0.07266755402088165, 0.022320717573165894, 0.0032652756199240685, 0.009604287333786488, 0.030458850786089897, 0.006119852885603905, 0.06225711107254028, -0.06654171645641327, 0.009446992538869381, -0.09543190151453018, -0.06172836571931839, 0.01...
10,010,029
Reversible binding of 5-(dimethylamino)-naphthalene-1-sulfonic acid (DNS) to human and bovine serum albumin has been monitored by changes in fluorescence intensity, wavelength maxima, and polarization. DNS has only one major binding site (ka = 5 x 10(6)) and one minor site (ka = 3 x 10(5)) on these proteins. The probe ...
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10,010,030
Identification and characterization of the direct folding process of hen egg-white lysozyme.
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10,010,031
Refolding kinetics of hen egg-white lysozyme (HEWL) have been studied by means of the stopped-flow method with guanidinium chloride as the denaturant. We show here that the three-species model U1 in equilibrium or formed from U2 in equilibrium or formed from N (U1 and U2 = unfolded; N = native) now established for panc...
[ -0.032697804272174835, -0.12145914137363434, 0.0323031023144722, -0.036898721009492874, 0.032251451164484024, 0.08637873828411102, -0.026171501725912094, 0.007503379601985216, 0.07842925190925598, 0.012814968824386597, -0.04647006094455719, 0.004961275029927492, -0.12522448599338531, 0.073...
10,010,032
Binding isotherms of nucleosides and polynucleotides measured by continuous ultrafiltration at constant volume: a rapid and precise technique.
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10,010,033
Equilibrium binding isotherms have been measured as a function of temperature for the interaction of 2- and 8-aminoadenosine with poly(U) by continuous, constant-volume ultrafiltration. An analytical method is described for determining the entire isotherm in a single experiment on a preequilibrated ligand--polymer solu...
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10,010,034
Reaction of antithrombin with proteases. Nature of the reaction with trypsin.
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10,010,035
Dopamine beta-hydroxylase: activity and inhibition in the presence of beta-substituted phenethylamines.
[ -0.004395660478621721, -0.08644668012857437, 0.0699712410569191, -0.007905831560492516, 0.0034599406644701958, 0.03430876135826111, 0.012281546369194984, -0.0424831360578537, 0.014356011524796486, 0.04713701829314232, -0.054590530693531036, 0.03846799209713936, -0.01371320616453886, 0.1023...
10,010,036
Solution structure of 5-keto-D-fructose: relevance to the specificity of hexose kinases.
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10,010,037
5-Keto-D-fructose (5KF) is isolated from cultures of Gluconobacter cerinus growing on D-fructose as the sole carbon source. 5KF is a substrate for hexokinase, fructokinase, and several polyol dehydrogenases. 1H and 13C nuclear magnetic resonance studies show that 5KF exists in different forms in anhydrous dimethyl-d6 s...
[ -0.08747636526823044, -0.1210169866681099, -0.10301003605127335, -0.020465148612856865, 0.06494266539812088, 0.04223035275936127, -0.05288625881075859, 0.046026065945625305, 0.04200783744454384, 0.0673384740948677, -0.02260240539908409, -0.0461064875125885, -0.12361720204353333, -0.0094409...
10,010,038
Effects of dietary iron deficiency of iron-sulfur proteins and bioenergetic functions of skeletal muscle mitochondria.
[ -0.010192343033850193, 0.02484535612165928, -0.024861041456460953, 0.13067524135112762, 0.012786222621798515, 0.05829077959060669, -0.014598856680095196, 0.026513902470469475, -0.0256365817040205, -0.07529379427433014, 0.023576663807034492, 0.006919954903423786, 0.03838404640555382, -0.047...
10,010,039
Studies on reduced and oxidized coenzyme Q (ubiquinones). II. The determination of oxidation-reduction levels of coenzyme Q in mitochondria, microsomes and plasma by high-performance liquid chromatography.
[ -0.04444824159145355, 0.049481101334095, -0.001575907925143838, 0.08325059711933136, 0.04468385875225067, 0.05869171395897865, -0.0351867713034153, 0.014043467119336128, 0.008548262529075146, -0.026628604158759117, 0.04065365344285965, 0.02953053079545498, 0.08690803498029709, 0.0224889572...
10,010,040
Reduced and oxidized coenzyme Q10 (Q10H2 and Q10) in guinea-pig liver mitochondria were rapidly extracted and determined by high-performance liquid chromatography (HPLC). The percentages of Q10H2 as compared to the total (sum of Q10 and Q10H2) were increased by the addition of respiratory substrates such as succinate, ...
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10,010,041
Release of mitochondrial respiratory control by cyanate salts.
[ -0.05457082390785217, 0.04814562574028969, -0.0024979165755212307, 0.0026606679894030094, -0.03121224418282509, 0.012199359945952892, -0.005274743307381868, -0.01051846519112587, 0.0041534509509801865, 0.0038194137159734964, 0.07048548012971878, 0.012222523801028728, 0.08922694623470306, -...
10,010,042
Electro-osmosis at the surface of phospholipid bilayer membranes.
[ -0.06206396594643593, -0.02568850852549076, 0.01119641587138176, -0.005696348380297422, 0.018019862473011017, -0.02361038327217102, 0.10235103964805603, 0.017914816737174988, 0.03920217230916023, -0.05437268689274788, 0.021114496514201164, -0.04593334347009659, -0.03811937943100929, 0.0720...
10,010,043
The electro-osmotic velocity is the velocity of a fluid near an interface produced by an electric field parallel to a surface. The velocity adjacent to fixed phospholipid bilayer membranes was measured by observing the velocity of small vesicles suspended in the fluid. The charge densities of the bilayers ranged from 0...
[ -0.06228137016296387, 0.0022915180306881666, 0.041297927498817444, -0.0071915024891495705, 0.02562841586768627, -0.03605125471949577, 0.031017348170280457, 0.06121646240353584, 0.027696339413523674, -0.02292490005493164, 0.03228805959224701, -0.1106785386800766, 0.004397600423544645, 0.098...
10,010,044
Role of diffusion potential on the flow of angiotensin II, bradykinin and related molecules through cellophane membranes.
[ 0.007496924605220556, -0.09610123932361603, -0.022604890167713165, 0.0014848951250314713, 0.04353208467364311, -0.0017475795466452837, -0.007439153268933296, 0.060770388692617416, 0.10415076464414597, -0.04171629995107651, -0.03963068872690201, 0.03801029548048973, -0.02284260466694832, 0....
10,010,045
The diffusion of electrically charged peptides (angiotensin II, bradykinin and [Suc1]angiotensin II) across tight cellophane membranes, obtained by different degrees of acetylation, shows a kinetic behaviour which was interpreted in the literature as indicative of the existence of different molecular conformations pres...
[ 0.003059094538912177, -0.1309259533882141, -0.03459654375910759, -0.02665931172668934, -0.004963400773704052, -0.055830132216215134, 0.02700929343700409, 0.06373566389083862, 0.09471718966960907, 0.025036659091711044, 0.04234972596168518, -0.012279411777853966, -0.07124719023704529, 0.1061...
10,010,046
Radioimmunoassay of digitonin.
[ -0.0455353707075119, 0.024976644665002823, -0.08333031088113785, -0.018189145252108574, -0.1186603456735611, -0.0295723769813776, 0.08524155616760254, 0.10634510219097137, -0.04475392401218414, -0.024480711668729782, 0.03811453655362129, -0.004513837862759829, 0.0422428622841835, 0.1253408...
10,010,047
We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 micrograms/ml (...
[ -0.06442130357027054, -0.07044341415166855, -0.04658075049519539, -0.03136609122157097, -0.10380902886390686, -0.030674347653985023, 0.018348606303334236, 0.08711977303028107, -0.02137627825140953, -0.03729788213968277, 0.009512154385447502, -0.020927036181092262, -0.007026198785752058, 0....
10,010,048
Alamethicin-induced changes in lipid bilayer morphology.
[ -0.06558787822723389, -0.08134102821350098, 0.05571331828832626, 0.033417537808418274, -0.08390858769416809, -0.0011723502539098263, 0.017053555697202682, 0.011911624111235142, 0.041193872690200806, -0.06965803354978561, 0.053926143795251846, -0.05465161055326462, 0.0025399283040314913, 0....
10,010,049
We have found that alamethicin, in the absence of an electric field, modifies both the hydrophilic surface and hydrophobic core of lipid bilayers. As shown by freeze-fracture and X-ray diffraction experiments with multiwalled vesicles, alamethicin increases the fluid space between bilayers by as much as 50 nm, and at t...
[ -0.09715471416711807, -0.08013992756605148, 0.15287120640277863, 0.0006159568438306451, -0.04792062193155289, 0.034621186554431915, 0.021997230127453804, 0.045274123549461365, 0.03170599043369293, -0.08451515436172485, -0.04309845343232155, -0.06579187512397766, -0.008034823462367058, 0.06...
10,010,050
Biphasic uptake of iron-transferrin complex by L1210 murine leukemia cells and rat reticulocytes.
[ -0.005349166691303253, -0.06682571768760681, -0.04426302760839462, 0.019560519605875015, 0.044433217495679855, -0.0005543187144212425, 0.07119952142238617, 0.02863808162510395, 0.06032286584377289, -0.040240634232759476, -0.037868890911340714, 0.05631082132458687, -0.016310354694724083, 0....
10,010,051
The kinetics of the cellular uptake of iron-transferrin complex was studied in L1210 murine leukemia cells and rat reticulocytes using 125I-transferrin. Saturation of transferrin with iron was necessary for optimal uptake. Following the incubation of cells with the radiolabeled complex a biphasic pattern of uptake was ...
[ -0.022394640371203423, -0.08421934396028519, -0.08932077139616013, 0.042738113552331924, -0.0037842493038624525, 0.03127323463559151, 0.06438176333904266, 0.06939581781625748, 0.05870575085282326, 0.02633773908019066, -0.04352084547281265, 0.06109073758125305, -0.009188778698444366, 0.0378...
10,010,052
Identification of exposed surface glycoproteins in undifferentiated and differentiated mouse N-18 neuroblastoma cells.
[ -0.04744822159409523, -0.02031264454126358, -0.039029210805892944, -0.02227642573416233, -0.008695997297763824, 0.03472978621721268, 0.022631749510765076, -0.019014451652765274, -0.011842132546007633, -0.042468249797821045, 0.013835749588906765, 0.06591203808784485, -0.015822026878595352, ...
10,010,053
A simple method is described that permitted rapid isolation of plasma membranes from mouse N-18 neuroblastoma cells. The purified plasma membranes gave a 10-fold increase in the specific activity of incorporated [3H]fucose over that of the cell homogenate. The specific activities of two other membrane markers, 5'-nucle...
[ -0.02734510228037834, -0.04202282056212425, -0.12260134518146515, -0.007685516495257616, -0.010893578641116619, 0.016916979104280472, 0.006662796251475811, -0.00545865623280406, 0.032303862273693085, -0.049157530069351196, 0.006645812187343836, 0.0649062842130661, -0.01636623591184616, 0.0...
10,010,054
Na+-independent L-arginine transport in rabbit renal brush border membrane vesicles.
[ -0.015427890233695507, -0.16004790365695953, 0.0042364224791526794, -0.023041382431983948, 0.0030172469560056925, -0.019854702055454254, 0.11892222613096237, -0.018565811216831207, 0.017554951831698418, 0.05767405778169632, -0.01400980819016695, 0.045033007860183716, 0.032084256410598755, ...
10,010,055
Na+-independent L-arginine uptake was studied in rabbit renal brush border membrane vesicles. The finding that steady-state uptake of L-arginine decreased with increasing extravesicular osmolality and the demonstration of accelerative exchange diffusion after preincubation of vesicles with L-arginine, but not D-arginin...
[ -0.00679381750524044, -0.19917498528957367, 0.046016350388526917, -0.03188084438443184, -0.009459871798753738, -0.007921886630356312, 0.090269073843956, 0.03426740691065788, 0.023285560309886932, 0.06535074859857559, -0.017962981015443802, 0.06845018267631531, 0.044481102377176285, 0.09441...
10,010,056
Kinetic and thermodynamic studies of the fusion of small unilamellar phospholipid vesicles.
[ -0.042008452117443085, -0.06658496707677841, -0.03978867083787918, 0.010745522566139698, -0.008292189799249172, -0.01141833234578371, -0.005365198478102684, 0.0377541221678257, 0.05820899456739426, -0.03260058909654617, 0.019326284527778625, -0.0052631753496825695, -0.03811018541455269, -0...
10,010,057
Small phospholipid vesicles, prepared so as to minimize impurities, fuse relatively slowly resulting in the time-dependent development of a characteristic endotherm in differential scanning calorimetry and corresponding changes in the Raman spectrum. The stability of small vesicles towards fusion increases with increas...
[ -0.07078580558300018, -0.09105878323316574, -0.007087205536663532, 0.006647062953561544, 0.0037370973732322454, -0.01895858719944954, -0.017719224095344543, 0.06962557137012482, 0.0786667913198471, -0.08372627943754196, 0.0683392658829689, -0.06102169677615166, -0.07193001359701157, 0.0319...
10,010,058
Tightly (covalently) bound fatty acids in cell membrane proteins.
[ -0.030217047780752182, -0.05366956442594528, -0.06183204427361488, 0.020895259454846382, -0.04440774768590927, 0.057405371218919754, -0.026607226580381393, 0.03744520992040634, 0.06111736223101616, -0.0760345309972763, 0.012577087618410587, -0.06509803980588913, -0.08109618723392487, -0.00...
10,010,059
The synthesis and use of thionphospholipids in 31P-NRM studies of lipid polymorphism.
[ -0.02282533049583435, -0.07363805919885635, -0.10470518469810486, -0.013328569941222668, -0.04225825518369675, 0.08280888944864273, 0.015210418961942196, 0.03816099837422371, -0.007201302796602249, 0.041525665670633316, -0.014770032837986946, -0.01734400913119316, -0.092367023229599, 0.011...
10,010,060
Effect of glycophorin on lipid polymorphism. A 31P-NMR study.
[ -0.006046575028449297, -0.11307911574840546, -0.040469489991664886, -0.03517609462141991, -0.048604659736156464, 0.020573100075125694, -0.028735294938087463, 0.01779717579483986, -0.05949466675519943, -0.07648835331201553, 0.03971613943576813, 0.03503701835870743, -0.09741998463869095, 0.0...
10,010,061
(1) The effect of glycophorin, a major intrinsic glycoprotein of the human erythrocyte membrane, on lipid polymorphism has been investigated by 31P-NRM (at 36.4 MHz) and be freeze-fracture electron microscopy. (2) Incorporation of glycophorin into vesicles of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) results in t...
[ -0.01725839264690876, -0.14828933775424957, -0.04754767566919327, 0.006657567340880632, -0.006998294964432716, 0.0036909407936036587, 0.019091419875621796, 0.08110731095075607, -0.007733334321528673, -0.009163230657577515, -0.012189017608761787, 0.016900138929486275, -0.05682121962308884, ...
10,010,062
Dependence of membrane potential on Ca2+ transport in cultured cytotrophoblasts of human immature placentas.
[ -0.031646743416786194, -0.05280893296003342, -0.017385607585310936, 0.03457208722829819, -0.07884480804204941, 0.0044312248937785625, 0.09319853037595749, 0.06713731586933136, 0.07798278331756592, 0.04976256191730499, 0.010501382872462273, -0.08874748647212982, -0.05249733850359917, 0.0695...
10,010,063
The electrical membrane properties of cultured human cytotrophoblast were examined by means of a standard electrophysiological technique. The mean values of the membrane potential (Rm) and the membrane resistance in a physiological medium were around -49 mV and 12 M omega , respectively. The membrane potential was depe...
[ -0.027398716658353806, -0.029993781819939613, 0.011235177516937256, 0.07570281624794006, -0.10282077640295029, 0.006215013097971678, 0.052249494940042496, 0.056438691914081573, 0.11318305879831314, 0.030169274657964706, -0.006363543216139078, -0.07003738731145859, 0.01183683518320322, 0.03...
10,010,064
Immunoglobulin-lipid interaction. A model membrane study.
[ -0.0290329921990633, -0.10008101165294647, -0.04229757562279701, 0.014738212339580059, -0.09344541281461716, 0.028951570391654968, 0.08162036538124084, 0.09924709796905518, 0.03755263239145279, -0.018202263861894608, -0.08177616447210312, -0.016584543511271477, 0.0085765914991498, 0.100673...
10,010,065
We recently presented evidence (Vandenbranden, M., De Coen, J.L., Jeener, R., Kanarek, L. and Ruysschaert, J.M. (1981) Mol. Immunol. 8, 621-631) for the existence of two conformational rabbit serum IgG immunoglobulin isomers. In the present report, their capacity to interact with lipid is investigated in model membrane...
[ -0.06996241211891174, -0.12810838222503662, 0.018519729375839233, 0.040600791573524475, -0.03276316449046135, -0.016093572601675987, 0.10841820389032364, 0.09816167503595352, 0.05713760852813721, -0.01073615625500679, -0.03438809886574745, -0.04071245342493057, -0.04306689649820328, 0.1607...
10,010,066
Cholesterol movement between human skin fibroblasts and phosphatidylcholine vesicles.
[ 0.007541260216385126, -0.11100959032773972, -0.05475950613617897, -0.00805891864001751, -0.038707513362169266, -0.03456288203597069, 0.03247243911027908, 0.05601498857140541, 0.027250947430729866, 0.009731066413223743, 0.011336010880768299, 0.001024105353280902, -0.06357835233211517, 0.021...
10,010,067
Cholesterol readily exchanges between human skin fibroblasts and unilamellar phospholipid vesicles. Only a fraction of the exchangeable cholesterol and only 10-15% of the total cellular free cholesterol is available for net movement or depletion to cholesterol-free phosphatidylcholine vesicles. [14C]Cholesterol introdu...
[ -0.03348568081855774, -0.1395583152770996, -0.056650832295417786, -0.003904627403244376, -0.008619707077741623, -0.0036179081071168184, 0.028349099680781364, 0.06646854430437088, 0.032807838171720505, 0.02977188676595688, -0.01612435281276703, -0.024990061298012733, -0.06689152866601944, -...
10,010,068
Kinetic characteristics of bicarbonate-chloride exchange across the neonatal human red cell membrane.
[ 0.029380472376942635, -0.07981356233358383, -0.054318178445100784, -0.014448368921875954, -0.06506854295730591, 0.0035865185782313347, 0.10786326974630356, 0.10504459589719772, 0.07655410468578339, -0.020755166187882423, -0.07635825872421265, -0.055686455219984055, -0.04599345847964287, 0....
10,010,069
The kinetics of HCO-3/Cl- exchange across red cell membrane of newborn infants was studied using a stopped-flow rapid reaction apparatus with a glass pH electrode attached. The measured apparent permeability P is (1.35 +/- 0.08 (S.E.)). 10(-4) cm/s (n = 30) for newborns, compared with (3.1 +/- 0.4) . 10(-4) cm/s (n = 1...
[ 0.018291549757122993, -0.14154410362243652, -0.09647028893232346, -0.011375133879482746, -0.027270006015896797, 0.02799159288406372, 0.08859879523515701, 0.05194184184074402, 0.0352548211812973, 0.028646456077694893, -0.08191081136465073, -0.06220576912164688, -0.0024434649385511875, 0.034...
10,010,070
Freeze-fracture characterization of 'young' and 'old' human erythrocytes.
[ -0.023334886878728867, -0.05495050176978111, -0.012897273525595665, 0.043159082531929016, -0.05947920307517052, 0.03973396494984627, 0.029264308512210846, 0.11240318417549133, -0.02247518301010132, 0.009226305410265923, -0.06630802154541016, -0.010004011914134026, -0.05382732301950455, 0.0...
10,010,071
While 'young' and 'old' erythrocytes separated by density show differences in various biochemical properties, the membrane ultrastructure as demonstrated by freeze-fracture remains unchanged. This implies that although superficial membrane components may be affected by aging, and whole segments of membrane may be lost,...
[ -0.0272932518273592, -0.054338548332452774, -0.02929224818944931, 0.0320219062268734, -0.03182201832532883, 0.03223561495542526, 0.03595734387636185, 0.07084988802671432, -0.0123175960034132, -0.03601223975419998, -0.01940671168267727, 0.010155119933187962, -0.06524493545293808, 0.05943838...
10,010,072
A rapid and sensitive assay for determination of cholesterol in membrane lipid extracts.
[ -0.03746608644723892, -0.0829472616314888, -0.08017274737358093, 0.00498247193172574, -0.07428400218486786, 0.014883192256093025, -0.011124277487397194, 0.042289555072784424, 0.0020958730019629, -0.06630774587392807, 0.031472399830818176, -0.006358094979077578, -0.04386729747056961, -0.053...
10,010,073
A commercially available enzymatic assay (Boehringer Monotest) was modified to allow a rapid and sensitive determination of cholesterol in membrane lipid extracts. This was achieved by adding 0.5% Triton X-100 to the reagent solution. The detergent did not interfere with the assay. The relationship between the amount o...
[ -0.031854111701250076, -0.08155614882707596, -0.07098400592803955, -0.047450244426727295, -0.07558060437440872, -0.02524414099752903, -0.029158711433410645, 0.07060214132070541, -0.03961396962404251, -0.07055128365755081, 0.03473789244890213, -0.013290983624756336, -0.06221379339694977, -0...
10,010,074
The erythrocyte membrane in essential hypertension. Modified temperature-dependence of Li+ efflux.
[ -0.013729274272918701, -0.09439553320407867, -0.08106288313865662, 0.09109414368867874, 0.05733755603432655, 0.03105049952864647, 0.047001831233501434, 0.011250960640609264, 0.000011808080671471544, -0.07498494535684586, 0.014923806302249432, -0.047170933336019516, 0.01629241369664669, 0.0...
10,010,075
The rate of ouabain-resistant Li+-efflux was studied in erythrocytes of normal controls and of patients with essential hypertension. Despite variability in rate, erythrocytes from normotensive persons revealed a uniform pattern of temperature dependence of the efflux, with two slopes (K = 9.4 and 19.1 kcal/mol, respect...
[ -0.017262503504753113, -0.0777062475681305, -0.08800364285707474, 0.08294257521629333, -0.03509073704481125, 0.038039226084947586, -0.003073018044233322, 0.02007974497973919, 0.02416977286338806, -0.10575702041387558, 0.005225272383540869, -0.06443104147911072, 0.027967775240540504, 0.0055...
10,010,076
Equilibrium binding of amphotericin B and its methyl ester and borate complex to sterols.
[ 0.002643462037667632, -0.02264656126499176, -0.10042399913072586, -0.037901245057582855, -0.04535665735602379, 0.010307925753295422, 0.005488102324306965, -0.0035492083989083767, 0.04624433070421219, -0.025568492710590363, 0.025815505534410477, -0.010197819210588932, -0.016992131248116493, ...
10,010,077
Scatchard analysis of the binding of amphotericin B to sterols in egg phosphatidylcholine/sterol vesicles revealed that amphotericin B was bound to ergosterol by approximately one order of magnitude more tightly than to cholesterol. Amphotericin B methyl ester formed a tighter complex with each of the sterols investiga...
[ 0.01784510351717472, -0.04557494819164276, -0.07258328050374985, -0.015199110843241215, -0.032877445220947266, -0.014361429959535599, -0.009120553731918335, -0.009253700263798237, 0.03229828178882599, -0.03286326304078102, 0.002094607800245285, 0.019643941894173622, -0.01835920289158821, -...
10,010,078
A hepatic membrane-associated factor stimulates nuclear DNA synthesis in cultured fibroblastic cells.
[ 0.03423410281538963, -0.06430529803037643, -0.08376691490411758, 0.03632671758532524, -0.005699263419955969, 0.006980791222304106, 0.004108543042093515, 0.007268017157912254, -0.028613293543457985, 0.013966104947030544, 0.019597139209508896, 0.006852773483842611, -0.01442630309611559, -0.0...
10,010,079
Effects of hydroxyurea on DNA synthesis in mouse L-cells.
[ -0.03829601779580116, -0.025036903098225594, 0.004610784817487001, -0.06114252656698227, -0.08029869198799133, 0.0640794113278389, -0.03652811422944069, 0.02239035628736019, -0.037992384284734726, 0.014900333248078823, -0.017136404290795326, 0.054274849593639374, -0.014523149468004704, -0....
10,010,080
The effect of the anti-metabolite hydroxyurea on DNA synthesis in mouse L-cells has been examined. It was shown previously that when DNA synthesis was diminished to very low levels by treatment with the drug there was preferential incorporation of added [3H]dThd into low molecular weight fragments (Martin, R.F., Radfor...
[ -0.05597341060638428, 0.008761313743889332, -0.0020594813395291567, -0.07892195135354996, -0.05037081241607666, -0.024271132424473763, -0.041862279176712036, 0.11096923053264618, -0.012378384359180927, -0.0013859836617484689, 0.032582271844148636, 0.09307150542736053, -0.0017985656158998609,...
10,010,081
Inhibition of DNA synthesis and cell death.
[ -0.014316513203084469, 0.05582715943455696, -0.06716527789831161, -0.012362319976091385, -0.02426989935338497, 0.006751943379640579, -0.002894188743084669, 0.046489275991916656, 0.03260551765561104, 0.08903304487466812, -0.0033623669296503067, 0.056009478867053986, 0.0029222434386610985, -...
10,010,082
The association between DNA synthesis inhibition and cell death in mouse L-cells was investigated using the drug hydroxyurea. This drug produces a preferential labelling of low molecular weight DNA and dose-response studies revealed a correlation between this effect and cytoxicity. Investigation of the reassociation ki...
[ -0.010336372070014477, -0.013586597517132759, -0.004446992184966803, -0.08782923221588135, -0.0933086946606636, 0.014234980568289757, 0.004959402605891228, 0.09563259780406952, 0.004291212186217308, 0.015831030905246735, 0.00038001235225237906, 0.09165819734334946, 0.04353129863739014, -0....
10,010,083
The effect of canavanine on protein synthesis and protein degradation in IMR-90 fibroblasts.
[ 0.01096991915255785, -0.04750336334109306, -0.07715670019388199, -0.06492798030376434, -0.008387534879148006, 0.03315161168575287, -0.039106011390686035, 0.07262944430112839, 0.021566588431596756, 0.05101083219051361, -0.002094677882269025, 0.0777454599738121, 0.029472745954990387, 0.08807...
10,010,084
Proteins of IMR-90 fibroblasts incorporating [35S]methionine during a 1 h labelling period in the presence of the arginine analogue canavanine were degraded twice as rapidly in the cells as were proteins similarly made in the presence of arginine. Using both isoelectric focusing and SDS-polyacrylamide gel electrophoret...
[ 0.013965558260679245, -0.12349396198987961, -0.08154617995023727, -0.04176165908575058, 0.002239350927993655, 0.03501783311367035, 0.05105756223201752, 0.12499972432851791, 0.054308220744132996, 0.009153569117188454, 0.026233859360218048, 0.06291494518518448, 0.05507642403244972, 0.1162300...
10,010,085
Low-angle X-ray scattering analysis of the Thermoplasma acidophilum nucleoprotein subunit.
[ -0.039516631513834, -0.011626658961176872, -0.044034138321876526, -0.0027363693807274103, 0.04207409918308258, -0.008035537786781788, -0.024109501391649246, 0.06703642010688782, 0.014200788922607899, -0.03648070991039276, 0.02183489501476288, -0.011776110157370567, 0.03984362632036209, 0.0...
10,010,086
DNA fork displacement rates in Bloom's syndrome fibroblasts.
[ -0.013371741399168968, -0.022664783522486687, 0.03135170415043831, -0.019254028797149658, -0.09014992415904999, 0.007066532038152218, -0.012159155681729317, 0.1079128161072731, 0.028707899153232574, 0.02654360421001911, 0.04045545309782028, 0.08122418820858002, -0.06053603067994118, 0.0232...
10,010,087
Peptide mapping of fat cell membrane substrates for cholera toxin-catalyzed ADP-ribosylation.
[ 0.013411660678684711, -0.0976770892739296, -0.06552073359489441, -0.008432019501924515, 0.05331544950604439, 0.009909717366099358, -0.03567809239029884, 0.02503926120698452, 0.06273786723613739, -0.0389951728284359, 0.01765410043299198, 0.008281536400318146, -0.016792815178632736, 0.106336...
10,010,088
Incubating rat fat cell membranes with [32P]NAD+ and cholera toxin results in ADP-ribosylation of three distinct components with approximate molecular weights of 42,000, 46,000 and 48,000. Partial proteolytic peptide maps of the Mr = 46,000 and 48,000 toxin-specific substrates generated by elastase, alpha-chymotrypsin,...
[ 0.046298474073410034, -0.09277299791574478, -0.08221381902694702, -0.04447537288069725, 0.05268942937254906, 0.041002579033374786, -0.02593131922185421, 0.08044890314340591, 0.06441900134086609, -0.013657104223966599, 0.009691908024251461, -0.010687656700611115, 0.03496775031089783, 0.1511...
10,010,089
Reversible depression of the reticuloendothelial system by liposomes.
[ 0.0345195010304451, -0.055101606994867325, -0.03667907789349556, 0.07610250264406204, 0.0018302993848919868, 0.01967420056462288, -0.032500505447387695, 0.05327954888343811, 0.05371247977018356, 0.07566475123167038, -0.05662081763148308, 0.038715660572052, -0.009641828946769238, 0.04305800...
10,010,090
The effect of various doses of different types (reverse phase evaporation vesicles and small unilamellar vesicles) of intravenously injected liposomes on reticuloendothelial activity, as measured by the blood clearance rate of intravenously injected carbon, was investigated. Also the effect of pretreatment with reverse...
[ 0.07030411064624786, -0.055118829011917114, 0.008445448242127895, 0.08795484900474548, -0.005206377245485783, -0.04583737999200821, -0.04616216942667961, 0.12357739359140396, 0.02210542932152748, 0.017646625638008118, 0.00455893762409687, 0.025188958272337914, 0.020305272191762924, 0.05468...
10,010,091
Mitochondrial damage in galactosamine-induced liver intoxication in rats.
[ 0.05808091163635254, -0.06906595081090927, 0.008471430279314518, 0.05930038169026375, 0.012818078510463238, -0.016384804621338844, -0.013510895892977715, 0.06540787220001221, -0.0520484521985054, 0.0504019521176815, 0.004001912660896778, 0.011973359622061253, 0.07641702890396118, 0.0158582...
10,010,092
Mitochondria isolated from livers of rats which received D-galactosamine (375 mg/kg body wt., four times) demonstrated a marked decrease in respiratory control ratios, the ADP/O ratios, and state 3 respiration rates and an increase in state 4 respiration rates. The aberration was profound with site I being altered prio...
[ 0.024852128699421883, -0.07760989665985107, -0.052369073033332825, 0.02181452326476574, 0.0483684279024601, 0.01844668760895729, -0.028923632577061653, 0.03774780035018921, -0.02225564233958721, 0.04001184552907944, -0.002155324211344123, -0.007639854680746794, 0.051294051110744476, 0.0074...
10,010,093
A proton nuclear magnetic resonance study of the binding of methylmercury in human erythrocytes.
[ 0.05234598368406296, -0.04154184088110924, -0.10199610143899918, 0.006833409424871206, -0.05905861780047417, 0.04201485589146614, 0.0357913076877594, 0.016286665573716164, -0.001146591268479824, -0.0980248898267746, 0.00044636998791247606, -0.011695299297571182, -0.0020864829421043396, -0....
10,010,094
The binding of methylmercury, CH3Hg(II), by small molecules in the intracellular region of human erythrocytes has been studied by 1H-NMR spectroscopy. To suppress or completely eliminate interfering resonances from the much more abundant hemoglobin protons, spectra were measured by a technique based on the transfer of ...
[ 0.030163975432515144, -0.09870642423629761, -0.11306595802307129, -0.05073339492082596, -0.011327017098665237, 0.004374157637357712, -0.022483156993985176, 0.038547731935977936, 0.024748992174863815, -0.07194339483976364, -0.022925689816474915, 0.029507098719477654, 0.00035457490594126284, ...
10,010,095
Influenza virus: an NMR study of mechanisms involved in infection.
[ -0.06268233060836792, -0.029023470357060432, -0.0760522410273552, -0.04499008506536484, 0.011624799109995365, 0.07579810917377472, -0.06922262907028198, 0.006909547373652458, 0.04803134500980377, 0.062045373022556305, -0.014490208588540554, 0.06877215206623077, 0.06997282058000565, 0.09723...
10,010,096
High resolution 1H-NMR spectroscopy has been used to study the infection of chicken embryo fibroblasts by influenza virus. Marked changes in the NMR spectrum occur when infectious influenza virus is introduced into the fibroblasts and these changes appear to depend upon the presence of active neuraminidase (EC 3.2.1.18...
[ -0.09802568703889847, -0.1221376582980156, -0.08387434482574463, -0.06962219625711441, 0.04995277523994446, 0.03076392039656639, -0.03370840847492218, 0.020117565989494324, 0.07554014027118683, 0.0743589773774147, -0.006188887171447277, 0.048466503620147705, 0.02729021944105625, 0.12044323...
10,010,097
Measurement of the intracellular pH of blood platelets using a photolabel fluorescent probe.
[ -0.014019391499459743, -0.05166206508874893, -0.038238972425460815, -0.05113239213824272, -0.0156775563955307, -0.03133571520447731, -0.0006148715037852526, 0.07815734297037125, 0.06013455614447594, 0.0015071133384481072, 0.0337497815489769, -0.016674663871526718, -0.04217018932104111, 0.0...
10,010,098
The internal pH of blood platelets using the intracellular photolabel probe azidofluorescein diacetate was determined. No change of intracellular pH during thrombin activation of human platelets was observed. Platelets were found to adjust themselves very quickly to the external pH. Quantitative subcellular localizatio...
[ -0.01391484122723341, -0.0895640105009079, -0.08870987594127655, -0.024886813014745712, 0.030323602259159088, -0.028024597093462944, 0.05287713557481766, 0.13933689892292023, 0.0698537528514862, 0.05061919987201691, 0.05849720537662506, -0.036921434104442596, -0.07290695607662201, 0.129909...
10,010,099
The effect of the cryoprotectants, dimethylsulfoxide and glycerol on water transport in the human red blood cell.
[ -0.001525749103166163, -0.03330341354012489, -0.049023352563381195, 0.04746457189321518, -0.043914858251810074, 0.04789595678448677, -0.014725558459758759, 0.07934383302927017, 0.02412354201078415, -0.059413328766822815, -0.04819053038954735, 0.022695649415254593, -0.07436338812112808, 0.0...
10,010,100
The response of human red blood cells to the cryoprotective agents, DMSO and glycerol, has been investigated using a pulsed NMR method. The experimentally determined parameters are: (1) the intracellular transverse relaxation time, T2a; (2) the mean residence time of intracellular water, tau a, which is effectively a r...
[ -0.03022157773375511, -0.09132954478263855, -0.0557684600353241, 0.004008618649095297, -0.07057324051856995, -0.012158066965639591, -0.03407704830169678, 0.0830858051776886, 0.024648822844028473, -0.03051779419183731, -0.035726260393857956, 0.03468269482254982, -0.04846442863345146, 0.0612...
10,010,101
Iron utilization in rabbit reticulocytes. A study using succinylacetone as an inhibitor or heme synthesis.
[ 0.13073427975177765, -0.06684444844722748, -0.05547764524817467, 0.14863480627536774, 0.0387239083647728, 0.0407269112765789, 0.06202073022723198, 0.05759624019265175, -0.01316311489790678, 0.021288959309458733, -0.03832982853055, 0.06152501329779625, 0.006538511253893375, 0.03269269689917...
10,010,102
We have investigated the effect of succinylacetone (4,6-dioxoheptanoic acid) on hemoglobin synthesis and iron metabolism in reticulocytes. Succinylacetone, 0.1 and 1 mM, inhibited [2-14C]glycine incorporation into heme by 91.2 and 96.4%, respectively, and into globin by 85 and 90.2%, respectively. 60 microMM hemin comp...
[ 0.05037343129515648, -0.06179865822196007, -0.09532256424427032, 0.01229992974549532, 0.06429215520620346, 0.08020645380020142, 0.012326187454164028, 0.0808088630437851, -0.033131569623947144, -0.004223078489303589, -0.040845200419425964, 0.049326661974191666, 0.0295852217823267, -0.027005...