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10,001,600 | Donated food staples available to hospitals under federal program. | [
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10,001,601 | Design trick: guessing right for a future that's anybody's guess. | [
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10,001,602 | Correlation between in vivo anti-Pseudomonas and anti-Candida activities and clearance of carbon by the reticuloendothelial system for various muramyl dipeptide analogs, using normal and immunosuppressed mice. | [
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10,001,603 | A linear correlation coefficient analysis, comparing in vivo anti-infective and reticuloendothelial stimulating activity of several different analogs of N-acetylmuramyl-L-alanyl-D-isoglutamine (muramyl dipeptide) suggests that the macrophage is an important target cell for these immunomodulating compounds. The increase in protection against infections of Candida albicans or Pseudomonas aeruginosa in normal or immunosuppressed mice after treatment with 18 different glycopeptides was found to correlate with the degree of clearance of colloidal carbon particles from the blood by the reticuloendothelial system after treatment with the same muramyl dipeptide analogs. The compound which gave the greatest protection in all four assays was N-acetylmuramyl-L-alpha-aminobutyryl-D-isoglutamine followed by N-acetyl-nor-muramyl-L-alanyl-D-isoglutamine. Both analogs were better than the parent muramyl dipeptide. Whether macrophage stimulation alone is responsible for the anti-infective properties of these compounds has not yet been determined. | [
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10,001,604 | N-acetyl muramyl dipeptide stimulation of bone resorption in tissue culture. | [
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10,001,605 | N-Acetyl-muramyl-L-alanyl-D-isoglutamine (MDP), a structurally defined fragment of bacterial peptidoglycan, stimulated significant release of previously incorporated 45Ca from fetal rat bones in tissue culture over the concentration range of 0.1 to 10.0 micrograms/ml. MDP-Stimulated bone resorption was not inhibited by the addition of the prostaglandin synthetase inhibitor indomethacin to the culture medium. MDP was neither mitogenic for nor stimulated the release of osteoclast-activating factor from cultured human peripheral blood mononuclear cells. Thus, MDP-stimulated bone resorption in vitro is mediated by a mechanism which is not dependent upon prostaglandins or osteoclast-activating factor. 6-O-Stearoyl-N-acetyl-muramyl-L-alanyl-D-isoglutamine, a lipophilic analog of MDP, was slightly more potent than MDP. Two diastereomers of MDP, N-acetyl-muramyl-L-alanyl-L-isoglutamine and N-acetyl-muramyl-D-alanyl-D-isoglutamine, which are inactive as adjuvants, were at least 1,000 times less active than MDP in stimulating bone resorption. The stereochemical specificity for bone-resorptive activity paralleled that required for adjuvant activity, macrophage activation, and activation of the reticuloendothelial system. | [
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10,001,606 | Transfer of resistance with syphilitic immune cells: lack of correlation with mitogenic activity. | [
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10,001,607 | Hamsters infected intradermally with Treponema pallidum Bosnia A develop extensive chronic skin lesions, usually accompanied by metastatic lesions involving the paws, lips, and anal region and by lymph nodes teeming with treponemes. Throughout the course of syphilitic infection, cells from the inguinal lymph nodes responded poorly to stimulation with suboptimal, optimal, or supraoptimal concentrations of concanavalin A, phytohemagglutinin P, or lipopolysaccharide. The response of syphilitic spleen cells was variable. Depression of lymphocyte reactivity to mitogens preceded clinical signs of infection and correlated well with the chronicity of syphilitic infection. When syphilitic hamsters were treated with a curative dose of penicillin, their mitogenic responses returned to normal or were slightly elevated. No correlation existed between mitogenic activity and the ability of lymphoid cells to induce an effective immune response when transferred to normal recipients. No significant differences in protection were detected among recipients of immune cells with or without activity to mitogens. These results demonstrate that lymphocyte transformation by mitogens in vitro is not a measure of effective treponemicidal activity and so may not be a valid indicator of the protective immune status of syphilitic animals. | [
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10,001,608 | Comparison fo agglutinin titers for Streptococcus mutans in tears, saliva, and serum. | [
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10,001,609 | The agglutinin titers for three Streptococcus mutans serotypes (AHT, BHT, and 10449, representing serotypes a, b, and c, respectively) were measured in the saliva, tears, and serum of 19 human subjects. Naturally occurring S. mutans agglutinins were routinely present in all fluids tested in the absence of overt local stimulation by antigen. The immunoglobulin A nature of this secretory agglutinin activity was suggested by blocking with alpha heavy-chain-specific antiserum and by the demonstration of S. mutans-reactive immunoglobulin A in the saliva and tears by indirect immunofluorescence. This finding is consistent with stimulation and antigen commitment of immunoglobulin A precursor lymphocytes at remote sites and subsequent homing to the lacrimal system. The relationship of anti-AHT agglutinins to anti-10449 agglutinins differed among the body fluids tested. The tears had more agglutinins for strain AHT than for strain 10449, whereas the reverse was true for saliva and serum. A possible explanation is local antigen-driven expansion of AHT-reactive committed lymphocytes in the lacrimal tissues. | [
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10,001,610 | Dual role of the immune response in street rabiesvirus infection of mice. | [
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10,001,611 | The cellular and humoral immune responses of mice to footpad injection of salivary gland suspensions of street rabiesvirus were investigated. Suppression of these responses with cyclophosphamide both increased the overall mortality rate from (50 to 100%) and delayed onset of disease signs and death for 1 to 2 weeks. Despite the absence of disease signs in these immunosuppressed animals, virus was present in the central nervous system, as shown by fluorescent-antibody staining of corneal epithelium. The onset of paralysis after limited immunosuppression was temporally related to a return to immune responsiveness, and passive transfer of homologous immune serum to infected immunosuppressed animals brought about their early paralysis and death. These findings indicate the importance of the immune response in the pathogenesis of street rabiesvirus infection. | [
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10,001,612 | Effect of nitrogen mustard on natural history of right-sided streptococcal endocarditis in rabbits: role for cellular host defenses. | [
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10,001,613 | Cellular host defenses are considered to be ineffective in bacterial endocarditis; the microorganisms in infected vegetations are protected from phagocytic cells by dense layers of fibrin. To test this hypothesis, nitrogen mustard-induced agranulocytosis and leukopenia were produced in rabbits with right-sided streptococcal endocarditis. The spontaneous sterilization of tricuspid infection observed in the control animals was not present in the granulocytopenic, leukopenic animals. Since the bacterium Streptococcus intermedius is not sensitive to the complement-mediated bactericidal effect of serum and since the animals were not bacteremic during the time of agranulocytosis, an inhibitory effect of the drug on local cellular host defense mechanisms is postulated. We suggest that the spontaneous sterilization of infective endocarditis in the right side of the heart in rabbits is mediated by cellular host defenses. | [
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10,001,614 | Characterization of an inducible bacteriophage from a leukotoxic strain of Actinobacillus actinomycetemcomitans. | [
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10,001,615 | A bacteriophage, designated phi Aa17, was isolated by mitomycin C induction from leukotoxic Actinobacillus actinomycetemcomitans strains 651. Electron microscopy of the virus revealed particles with regular, nonelongated, polyhedral heads, and tails consisting of a contractile sheath and core. Spikes emanated from the base of the tail. The head had a diameter of 70 nm. The fully extended tail sheath had a length of 127 nm and a diameter of 22 nm. In its contracted form, the tail sheath measured 47 nm in length and 25 nm in diameter. The phage had a buoyant density of 1.370 in CsCl, and its genome was found to be double-stranded DNA. A single-cycle growth curve revealed that the phage had a latent period of 30 min and a burst size of 435 PFU per cell. The host range of the phage was examined, and A. actinomycetemcomitans strains ATCC 29523 and ATCC 29524 were found to be phage sensitive, whereas strains Y4, ATCC 29522, 2043, 652, 651, 627, 2097, N27, 2112, and 511 were resistant. The host range of this virus does not suggest any association between the phage and leukotoxin production. | [
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10,001,616 | Ultrastructure of Gram-negative cotton bacteria with different pulmonary toxicities. | [
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10,001,617 | The shedding of outer membrane material was observed by electron microscopy in typical gram-negative cotton bacteria, except Agrobacterium sp. This finding is in accordance with the relative pulmonary toxicities of these bacteria. The presence of capsules did not seem to be correlated with the acute pulmonary toxicity of the bacteria. | [
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10,001,618 | Depression of monocyte and polymorphonuclear leukocyte oxidative metabolism and bactericidal capacity by influenza A virus. | [
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10,001,619 | Decreased host defense against bacterial disease associated with influenza infection may be related to virus-induced changes in phagocytic cell function. Influenza A virus initiates the respiratory burst in peripheral blood monocytes and polymorphonuclear leukocytes, with a peak chemiluminescent response approximately 3 min after virus is added to the cells in vitro. Electron micrographs of phagocytic cells incubated with influenza virus demonstrated virus attached to the cell membrane and within phagocytic vacuoles. After 20 min of incubation of the virus with phagocytic cells, the chemiluminescent response to opsonized zymosan or phorbol myristate acetate was decreased by 30 to 90%. Phagocytic activity of monocytes and polymorphonuclear leukocytes incubate with influenza virus was normal, but the bactericidal activity was significantly depressed. Influenza A virus therefore stimulates an oxidative burst in monocytes as well as polymorphonuclear leukocytes, leading to a subsequent depression of the oxidative metabolic response and bactericidal capacity of the phagocytic cells. | [
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10,001,620 | Fungistatic capacity of sera from guinea pigs injected with various iron solutions: differences between Trichophyton mentagrophytes and Rhizopus oryzae. | [
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10,001,621 | The fungistatic capacity and serum ion levels (SI) of guinea pigs given subcutaneous injections of various iron solutions were examined. The administration of 2.0 ml of 0.1 M ferric ammonium sulfate, ferric sulfate, or ferric chloride subcutaneously had no significant effect on the SI 3 h after administration, whereas ferric ammonium citrate, ferric citrate, or ferrous sulfate elevated the SI to 50 to 140 times that necessary to saturate the unbound transferrin in normal sera. The sera from 11 of 15 guinea pigs with an elevated SI remained fungistatic for Trichophyton mentagrophytes, whereas 3 of 15 guinea pigs with an elevated SI remained fungistatic for Rhizopus oryzae. The sera from normal guinea pigs were consistently fungistatic for Rhizopus oryzae. The sera from normal guinea pigs were consistently fungistatic for both T. mentagrophytes and R. oryzae. These data suggest that subcutaneous administration of certain iron compounds can significantly elevate the SI without completely abolishing the fungistatic capacity of the serum. | [
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10,001,622 | Genetic control of Propionibacterium acnes-induced protection of mice against Babesia microti. | [
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10,001,623 | Using various strains of inbred mice, we found that Propionibacterium acnes-induced protection against the hemoprotozoan parasite Babesia microti was controlled by a dominant gene(s) not linked to the major histocompatibility gene (H2) complex of mice. P. acnes-induced resistance to infection was not merely an amplification of the normal immune response to B. microti, since innate resistance to infection was controlled by different genes. Expression of the nonspecific protection induced by P. acnes was found to be transferable with cells of bone marrow origin, and it did not necessarily accompany the induction of hepatosplenomegaly. The implication of these results for the understanding of the mechanism of P. acnes-induced protection is discussed. | [
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10,001,624 | Neutrophil chemotaxis by Propionibacterium acnes lipase and its inhibition. | [
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10,001,625 | The chemoattraction of Propionibacterium acnes lipase for neutrophils and the effect of lipase inhibitor and two antibiotic agents on the chemotaxis were evaluated. Of the various fractions tested, partially purified lipase (fraction 2c) was the most active cytotaxin produced by P. acnes. Serum mediators were not required for the generation of chemotaxis by lipase in vitro. Diisopropyl phosphofluoridate at low concentration (10(-4) mM) completely inhibited lipase activity as well as polymorphonuclear leukocyte chemotaxis generated by lipase. Tetracycline hydrochloride and erythromycin base at concentrations of 10(-1) mM and 1 mM, respectively, caused 100% inhibition of PMN migration toward lipase or zymosan-activated serum. The inhibiting activity of the antibiotics was directed against cells independently of any effect on lipase. Chemotaxis by P. acnes lipase suggests a wider role for this enzyme in the inflammatory process and the pathogenesis of acne vulgaris. | [
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10,001,626 | Cytotoxin of Pasteurella haemolytica acting on bovine leukocytes. | [
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10,001,627 | The toxicity of Pasteurella haemolytica culture supernatant for bovine and porcine cells was evaluated by 51Cr release. Sterile bacterial culture supernatant was toxic for bovine pulmonary lavage cells, peripheral blood lymphocytes and neutrophils, and cultured peripheral blood mononuclear cells, resulting in marked 51Cr release. Only slight release was induced from cultured Madin-Darby bovine kidney cells, porcine pulmonary lavage cells, peripheral blood mononuclear cells, lymphocytes, or neutrophils. No release was detected with primary bovine spleen cell cultures, bovine erythrocytes, or porcine erythrocytes. The demonstrated specificity of this cytotoxin for bovine leukocytic cells may be important in the pathogenesis of bovine pneumonic pasteurellosis. | [
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10,001,628 | Correlation of the development of acoustic trauma to the intensity and time of acoustic overstimulation. | [
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10,001,629 | Distribution and significance of norepinephrine in the lateral cochlear wall of pigmented and albino rats. | [
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10,001,630 | Picogram quantities of norepinephrine were found in cochlear regions of pigmented and non-pigmented rats. These regions of the cochlea were the modiolus, organ of Corti--osseous spiral lamina and the lateral cochlear wall. The content of norepinephrine in the modiolus and lateral cochlear wall of the pigmented rat was significantly greater than that in areas of the non-pigmented rat. In contrast, there was no statistical difference between the norepinephrine content of the organ of Corti--osseous spiral lamina region of the pigmented rat and that of the albino rat. Since a major difference between the pigmented and albino rats is the presence of melanin-containing melanocytes in the modiolus and lateral cochlear wall region of the pigmented animals, it is possible that norepinephrine is stored in cochlear melanocytes. | [
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10,001,631 | Acoustic reflex after experimental lesions to inner and outer hair cells. | [
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10,001,632 | Chronic effects of noise or kanamycin on the acoustic intra-aural reflex in nonanesthetized rabbits were studied. The intra-aural reflex activity was simultaneously recorded in both ears upon alternate stimulation of the left and the right side. The inner ears were analyzed in scanning and transmission electronmicroscopy. Exposure to bandlimited high-level noise was found to induce extensive deformations in the sensory hairs of the inner hair cells without loss of outer hair cells. Kanamycin caused a degeneration of outer hair cells in the basal 1-2 turns without affecting the morphology of the inner hair cells. The morphological changes, as a function of the location on the basilar membrane, were compared to physiological changes, as a function of frequency. In the noise-exposed animals the threshold of the intra-aural reflex was found to be raised in a frequency range corresponding to the extent of abnormal sensory hairs of the inner hair cells. In the kanamycin-treated animals reflex changes correlated to the loss of outer hair cells. The observations were interpreted as indicating that both types of hair cells cooperate in the activation of the intra-aural reflex in rabbits. | [
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10,001,633 | Cochlear microphonic evidence for mechanical propagation of distortion products (f2 - f1) and (2f1 - f2). | [
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10,001,634 | The present cochlear microphonic (CM) study was undertaken to help resolve a conflict in the literature regarding cochlear nonlinear properties. The CM studies of Dallos and his coworkers have concluded that "(up to 70-80 dB SPL [re 20 mu Pa]), all orders of distortion components...do not seem to be accompanied by traveling waves of their own" (Dallos, P. (1973): The Auditory Periphery: Biophysics and Physiology. Academic Press, New York). However, studies of spatial distributions of cochlear nerve fiber responses, acoustic distortion products in the ear canal, and related modeling studies of Kim et al. (Kim, D.O. and Molnar, C.E. (1975): in: The Nervous System, Vol. 3: Human Communication and Its Disorders. Editor: D.B. Tower. Raven Press, New York; Kim, D.O., Molnar, C.E. and Matthews, J.W. (1980): J. Acoust. Soc. Am. 67, 1704-1721) have led to conclusions to the contrary. In the present study, CM data were obtained from the second and third turns of the chinchilla cochlea using fluid-filled glass micropipettes in scala media and nichrome wire electrodes in scala vestibuli and scala tympani. We sought responses containing predominant distortion products (f2 - f1) and (2f1 - f2) by fixing a distortion frequency (fD) near the characteristic frequency (CF) of the recording site and varying the stimulus frequencies f1 and f2 and SPLs (with L1 = L2). By subsequently varying the distortion frequency around the CF, e.g., fixing f1 well above the CF and varying f2, we measured the tuning characteristics of the distortion products (f2 - f1) and (2f1 - f2). Tuning characteristics of single-tone responses were measured by applying single-tone stimuli of various frequencies with a constant SPL at the eardrum. We have observed, with SPLs as low as 25 dB, that these distortion products in CM display tuning similar to the single-tone response which is consistent with above neural results. From these tuning similarities, we conclude that our CM data reflect the presence of mechanically propagated distortion products at low SPLs, in agreement with the above studies by Kim et al. Validity of our results is supported by the sensitivity and sharp tuning of our CM data and, in the case of the scala media recordings, by the presence of a normal d.c. endolymphatic potential. Plausible explanations for the opposing conclusions of previous studies of Dallos et al. and the present study are discussed. | [
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10,001,635 | Corresponding effects of acoustic fatigue on the cochlear microphonic and the compound action potential. | [
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10,001,636 | Moderate levels of acoustic fatigue, usually induced by a 100 dB SPL pure tone at a frequency appropriate to the location of intracochlear differential electrodes, have a surprising and paradoxical influence on the cochlear microphonic and the compound action potential of the auditory nerve. While the low-level microphonic becomes smaller, the low-level action potential becomes considerably larger and exhibits a shortened latency. The high-level microphonic and the high-level action potential are left virtually unchanged at these levels of fatigue. Nonetheless, if the duration or intensity of exposure is increased, both the high-level and low-level action potentials decrease as well. Assuming that the low-level microphonics are generated by outer hair cells, these data suggest that one relationship between outer hair cell function and neural function is inhibition at low intensities. Assuming that the high-level microphonic tends to be generated by inner hair cells, the decrease in the action potential at all intensities whenever the high-level microphonic potential is impaired suggests that the functional relationship between inner hair cells and auditory nerve function is excitatory. | [
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10,001,637 | An electronmicroscopic study of microtubules in the development of marginal cells of the mouse stria vascularis. | [
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10,001,638 | This study provides an ultrastructural evaluation of cytoplasmic microtubules during the maturation of marginal cells of the mouse stria vascularis. Postnatal marginal cells are cuboidal in structure and over a period of days develop the numerous cellular processes typical of transporting epithelia. Immediately after birth, marginal cells contain numerous microtubules randomly oriented throughout the cytoplasm. Golgi bodies and vesicles are also abundant. The initiation of cellular extension is characterized by the presence of sheet-like extensions of plasma membrane about areas of the cell periphery. Subsequently, large organelle-containing processes form, whose plasmalemma appear pleated due to the presence of the above mentioned sheet-like extensions. Typically, these processes contain microtubules oriented parallel to the direction of their extension. Within these processes, microtubles are closely associated with organelles, such as mitochondria, and may function to displace and stratify these organelles within the processes. The number and length of microtubules increase as the processes grow larger. In the adult mouse, the strial marginal cell processes are attenuated and contain almost exclusively microtubules and mitochondria. The membrane pleats unfold, apparently to provide plasma membrane for cellular extension. The data strongly implicate microtubules in strial development. Furthermore, it is suggested that the depolymerization of microtubules in the adult may underlie strial atrophy. | [
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10,001,639 | Corresponding effects of hypoxia on the cochlear microphonic and the compound action potential. | [
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] |
10,001,640 | Mild hypoxia has an unexpected influence on the compound action potential (CAP) compared to its effect on the cochlear microphonic (CM). While the CM decreases in amplitude near threshold, the low-level CAP increases in amplitude by as much as 400% and decreases in latency and width. The magnitude of latency decrease is dependent on the center frequency of 1/3 octave band-filtered clicks used as stimuli. Below 13 500 Hz the latency is increasingly shortened at a rate of 0.073 ms/octave. At high intensities moderate hypoxia has no effect on either the CM or the CAP. However, with more severe hypoxia the high-level CM also begins to deteriorate. Correspondingly both the high- and low-intensity CAP decrease in magnitude and increase in latency relative to their control values. Assuming that the low-level CM is generated by outer hair cells, these results suggest that one relationship between outer hair cell function and auditory nerve function near function near threshold is that of inhibition. The fact that N1 latency is shortened suggests that this inhibition occurs in the basalward direction. | [
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10,001,641 | Relationship between temperature and growth rate of bacterial cultures. | [
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10,001,642 | The Arrhenius Law, which was originally proposed to describe the temperature dependence of the specific reaction rate constant in chemical reactions, does not adequately describe the effect of temperature on bacterial growth. Microbiologists have attempted to apply a modified version of this law to bacterial growth by replacing the reaction rate constant by the growth rate constant, but the modified law relationship fits data poorly, as graphs of the logarithm of the growth rate constant against reciprocal absolute temperature result in curves rather than straight lines. Instead, a linear relationship between in square root of growth rate constant (r) and temperature (T), namely, square root = b (T - T0), where b is the regression coefficient and T0 is a hypothetical temperature which is an intrinsic property of the organism, is proposed and found to apply to the growth of a wide range of bacteria. The relationship is also applicable to nucleotide breakdown and to the growth of yeast and molds. | [
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10,001,643 | Iron transport in Mycobacterium smegmatis: Uptake of iron from ferric citrate. | [
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10,001,644 | In mycobacterial growth medium 40 to 400 microM citrate was required to solubilize 2 microM 55Fe. This solubilized 55Fe was taken up into both iron-deficient and iron sufficient washed cell suspensions of Mycobacterium smegmatis and Mycobacterium bovis BCG. Although the 55Fe was taken up into the cell, the citrate was not. The uptake system with M. smegmatis was not inhibited by electron transport inhibitors, uncouplers of oxidative phosphorylation, or thiol reagents and was saturable with iron at approximately 35 microM. The system was independent of the iron transport systems already known to exist in M. smegmatis: i.e., the two exochelin routes of assimilation as well as the mycobactin-salicylate system. It was not induced by the presence of 400 microM citrate in the growth medium, nor did the presence of citrate in the medium affect the production of either exochelin or mycobactin. | [
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10,001,645 | Peptidoglycan of Rhodopseudomonas viridis: partial lack of N-acetyl substitution of glucosamine. | [
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10,001,646 | A lack of at least 70% of N-acetyl substitution of glucosamine in the glycan strands of the peptidoglycan from the gram-negative bacterium Rhodopseudomonas viridis is reported. A disaccharide, very likely GlcN beta(1 leads to 4) Mur, was observed in hydrolysates of the isolated peptidoglycan. The disaccharide was not observed when peptidoglycan was N-acetylated before hydrolysis. The peptidoglycan of R. viridis was resistant to lysozyme but became sensitive after N-acetylation with acetic anhydride. The disaccharide was found with peptidoglycan from all R. viridis strains investigated, as well as with R. sulfoviridis P1 and R. palustris strains, but not with peptidoglycan from R. gelatinosa, Rhodospirillum tenue, and Pseudomonas diminuta NCTC 8545. | [
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10,001,647 | Physiological characteristics of Thiomicrospira sp. Strain L-12 isolated from deep-sea hydrothermal vents. | [
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10,001,648 | Growth of the obligately chemolithotrophic Thiomicrospira sp. strain L-12, isolated from a hydrothermal vent at a depth of 2,550 m in the Galapagos Rift region, was optimal at pH 8 and required 200 mM Na+ and divalent ions (Ca2+ and Mg2+). The organism was microaerophilic and tolerated 300 microM sulfide without a decrease in the rate of CO2 incorporation. Growth and CO2 incorporation occurred within the temperature range of 10 to 35 degrees C, with both optimal at 25 degrees C. At the in situ pressure of 250 atm. the rate of CO2 incorporation was reduced by 25% relative to that measured at 1 atm: it was entirely suppressed at 500 atm. The results of this physiological characterization suggest that Thiomicrospira sp. strain L-12 can be an active autotroph in the hydrothermal environment. | [
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10,001,649 | Purification and properties of pyruvate kinase from Streptococcus mutans. | [
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10,001,650 | Pyruvate kinase (EC 2.7.1.40) from Streptococcus mutans strain JC2 was purified, giving a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight of the native enzyme was 180,000 to 190,000, and the enzyme was considered to consist of four identical subunits. This enzyme was completely dependent on glucose 6-phosphate for activity, and the saturation curve for activation by glucose 6-phosphate was sigmoidal. In the presence of 0.5 mM glucose 6-phosphate, the saturation curves for the substrates phosphoenolpyruvate and ADP were hyperbolic, and the Km values were 0.22 and 0.39 mM, respectively. GDP, IDP, and UDP could replace ADP, and the Km for GDP (0.026 mM) was 0.067 of that for ADP. The enzyme required not only divalent cations, Mg2+ or Mn2+, but also monovalent cations, K+ or NH4+, for activity, and it was strongly inhibited by Pi. When the concentration of Pi was increased, the half-saturating concentration and Hill coefficient for glucose 6-phosphate increased. However, the enzyme was immediately inactivated in a solution without Pi. The intracellular concentration of glucose 6-phosphate, in cooperation with that of Pi, may regulate pyruvate kinase activity in S. mutans. | [
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10,001,651 | Possible association of segregated lipid domains of Mycoplasma gallisepticum membranes with cell resistance to osmotic lysis. | [
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10,001,652 | Freeze-fracturing of cholesterol-rich Mycoplasma gallisepticum membranes from cells grown in a medium containing horse serum revealed particle-free patches. The patches appeared in cells quenched from either 4 or 37 degrees C. Particle-free patches also occurred in membranes of cells grown in a serum-free medium supplemented with egg-phosphatidylcholine but not in membranes of cells grown with dioleoylphosphatidylcholine. The appearance of particle-free patches was attributed to the presence of disaturated phosphatidylcholine (PC) molecules in M. gallisepticum membranes, which were synthesized by the insertion of a saturated fatty acid at position 2 of lysophosphatidylcholine derived from exogenous PC present in the growth medium. Consequences of the synthesis of the disaturated PC also included a decrease in osmotic fragility and the ability of the cells to be permeated by K+. Electron paramagnetic resonance and fluorescence polarization measurements revealed that the fluidity of the lipid domain in the protein-rich M. gallisepticum membranes was almost identical to that of an aqueous dispersion of M. gallisepticum membrane lipids. Furthermore, the electron paramagnetic resonance spectra of the membranes were single-component spectra showing no indication of immobilized regions. The possibility that the osmotic resistance of M. gallisepticum cells is associated with the particle-free patches rather than with a restricted membrane fluidity caused by membrane proteins is discussed. | [
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10,001,653 | Spontaneous protoplast formation in Methanobacterium bryantii. | [
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10,001,654 | Methanobacterium bryantii was found to undergo rapid lysis when grown in a prereduced chemically defined medium under H2-CO2 (4:1, vol/vol). The addition of 20 mM MgCl2 to the medium gave, rather than rapid lysis, a gradual formation of phase-dark spherical bodies which in thin section appeared as true protoplasts. In general, the protoplasts were stabilized by divalent but not monovalent cations and, unlike whole cells, were sensitive to lysis by Triton X-100. Electron microscopic examination revealed that protoplast formation was preceded by a general breakdown of the cell wall with an apparent squeezing out of the protoplast through the degraded wall. The growth of cells was greatly increased and not accompanied by detectable lysis in a medium modified by elevating the levels of nickel and ammonium. | [
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10,001,655 | Exospore formation in Methylosinus trichosporium. | [
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] |
10,001,656 | Formation of exospores in Methylosinus trichosporium was examined by electron microscopy; serial sectioning was used to visualize the shape and location of the developing exospore in relation to the vegetative cell. The initial stage was the formation of a budlike enlargement on one end of the vegetative cell. The enlargement was surrounded by the exospore capsule, and the cell wall was continuous around both the cell and the developing exospore. A constriction occurred in the area where the budlike structure was attached to the vegetative cell, and the constriction continued to form until the immature exospore was detached from the vegetative cell. The cup-shaped immature exospore was surrounded by the exospore capsule, which appeared to hold the exospore close to the vegetative cell. After separation from the vegetative cell, the immature exospore developed further by forming the exospore wall and by becoming spherical. | [
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10,001,657 | Production of deferriferrioxamines B and E from a ferroverdin-producing Streptomyces species. | [
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10,001,658 | When Streptomyces sp. strain Wak. A-305 is cultured in iron (III)- or cobalt (II)-containing media, tris(p-vinylphenyl-4-hydroxy-3-nitrosobenzoato-N3, O4)ferrate(II) (ferroverdin) or the analogous tris-cobalt(III) complex, respectively, is produced intracellularly. In contrast, when cultured in transition metal ion-limiting media. Streptomyces sp. strain Wak. A-305 did not produce p-vinylphenyl 4-hydroxy-3-nitrosobenzoate (deferroferroverdin). Instead, deferriferrioxamines B and E, which have been characterized from other streptomycetes, were isolated extracellularly and may act as siderophores in this organism.U | [
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10,001,659 | Induction of 3-hydroxybenzoate 2-hydroxylase in a Pseudomonas testosteroni mutant. | [
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10,001,660 | Benzoate was established as the inducer of a unique 3-hydroxybenzoate 2-hydroxylase activity found in a Pseudomonas testosteroni mutant which is unable to grow on m-hydroxybenzoate as its sole source of carbon and energy. | [
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10,001,661 | Methylamine uptake in Pseudomonas species strain MA: utilization of methylamine as the sole nitrogen source. | [
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10,001,662 | The uptake of methylamine as the sole nitrogen, but not carbon, source by Pseudomonas sp. strain MA was investigated. Under these growth conditions, a high-affinity, low-capacity uptake system was present having a Km of 16 microM and Vmax of 2 nmol.min-.mg (dry weight) of cells that was competitively inhibited by ammonium chloride. The transport system was induced by growth on succinate with methylamine as the sole nitrogen source. | [
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10,001,663 | Lipoteichoic acid, a major amphiphile of Gram-positive bacteria that is not readily extractable. | [
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10,001,664 | Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. | [
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10,001,665 | Glycolipids from some extreme thermophilic bacteria belonging to the genus Thermus. | [
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] |
10,001,666 | The lipids of Thermus aquaticus YT1, Thermus thermophilus HB8, Thermus sp. strains H and J (from Icelandic hot springs), and Thermus sp. strain NH (from domestic hot water) have been investigated. Each strain contained two major components, a glycolipid and a glycophospholipid, which have been isolated and analyzed. All of the strains contained as the principal component (41 to 57% of total lipid) a diacyldiglycosyl-(N-acyl)glycosaminylglucosylglycerol, but the five glycolipids differed in carbohydrate composition. The glycophospholipid appeared to be identical in each strain and contained an N-acylglucosamine residue. The principal fatty acids were C15 and C17 branched-chain compounds. This unique polar lipid composition should be of value in the classification of other thermophiles in the genus Thermus. The exceptionally high carbohydrate content of the lipids of these extreme thermophiles may be of significance in relation to the molecular basis of thermophily. | [
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10,001,667 | Physostigmine treatment of acute phencyclidine intoxication. | [
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10,001,668 | Involuntary admission--a century of experience. | [
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10,001,669 | Anxiety and mitral valve prolapse syndrome. | [
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10,001,670 | The diagnosis of Mitral Valve Prolapse Syndrome (MVPS), even though symptomatically similar to anxiety neurosis, may prompt pharmacological treatment only, neglecting any underlying emotional problems. If only a dichotomy were considered, one diagnosis might be seen as mutually exclusive of the other. A case history is presented illustrating features of both clinical syndromes. We consider the interaction of both etiologies, as well as the possibility of both being unrelated and coincidental. As physiological factors are explored when the diagnosis of anxiety neurosis is considered, so should psychological factors be examined with MVPS. | [
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] |
10,001,671 | A trial of dextroamphetamine in patients with involutional agitated depression. | [
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10,001,672 | Unusual presentation of manic depressive disorder in adolescence. | [
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10,001,673 | The importance of drug use histories in a series of alcoholics. | [
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10,001,674 | Visions of snakes. | [
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10,001,675 | The histamine-induced skin reaction in schizophrenic and normal subjects. | [
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10,001,676 | Most published data on histamine skin tests on schizophrenic patients show them to be insensitive relative to normals or other psychotic patients. However, experimental conditions have varied and in most cases only a single dose of histamine was used. We have compared 13 volunteer controls and 16 paranoid schizophrenics; each subject was injected intradermally with histamine, 10, 20, and 50 microgram, doses predetermined in volunteers to give a dose-response curve. Wheal and flare were measured at 10 and 20 minutes, intervals near the time of maximum flare. We found no statistically significant differences between normal controls and schizophrenic patients. | [
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] |
10,001,677 | Chemical characterization of thymosin beta 4. | [
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10,001,678 | As part of our ongoing investigations on the endocrine thymus, we have isolated and purified to homogeneity a hormone-like peptide which we have termed thymosin beta 4. Thymosin beta 4 has Mr = 4982 and an isoelectric point of 5.1. The complete amino acid sequence of this polypeptide has been established by automated Edman degradation as well as by manual sequence analysis. Thymosin beta 4 is composed of 43 amino acid residues with acetylserine at the NH2 terminus. This molecule induces expression of terminal deoxynucleotidyl transferase in transferase-negative murine thymocytes in vivo and in vitro. It also exhibits ability to inhibit the migration of macrophages. Comparison of the sequence of thymosin beta 4 to other thymic hormones or other published protein sequences does not reveal any statistically significant relationship. Two helical regions were identified in the structure using data for prediction of protein conformation. It is proposed that thymosin beta 4 is one of the biologically active peptides present in thymosin fractions 5 and 5A which participate in the regulation, differentiation, and function of thymus-derived lymphocytes and may also act directly or indirectly on macrophages and perhaps other cells involved in cell-mediated immunity. | [
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] |
10,001,679 | Characterization of polynucleotide phosphorylase from Micrococcus luteus and isolation of the 13,000 base poly(A) product of the polymerization reaction. | [
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10,001,680 | A new purification procedure for polynucleotide phosphorylase from freeze-dried Micrococcus luteus cells gives approximately 20% yield of nearly homogeneous, primer-independent enzyme which is free of nucleic acid. The physicochemical properties of M. luteus polynucleotide phosphorylase are similar to those previously described for the enzyme from Escherichia coli in terms of Mr, subunit structure, and amino acid composition. The purified enzyme appears to be a trimer composed of three identical subunits (Mr 92,000), but it probably does not exist as such in the cell. Ferguson plot analyses of enzyme in cell extracts indicate that prior to purification the enzyme exists in oligomeric forms characterized by both higher charge and greater Mr. Changes in size and charge of oligomers which occur during purification are probably due to the dissociation of proteins and/or nucleic acids. Dissociation of the oligomers is achieved by dilution and electrophoresis, but reassociation does not occur after concentration. The poly(A) product of the initial polymerization stages migrates as a single band on both nondenaturing and urea-agarose gels. It is 13,000 +/- 2,000 nucleotides long, as measured by electron microscopy, and 8,000 nucleotides long by gel electrophoretic analysis. This poly(A) product remains bound to the enzyme after synthesis, yet can be easily obtained free of protein by proteinase K digestion. | [
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10,001,681 | Estrogen-induced changes in chick oviduct membrane glycoproteins. | [
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] |
10,001,682 | Membrane protein and glycoprotein changes during estrogen-induced differentiation of the chick oviduct were studied. Membrane preparations from chicks treated with diethylstilbestrol for 3, 6, 10, and 20 days; from chicks treated with hormone for 10 days and then withdrawn for 2 days; and from mature hens were incubated with either GDP-[14C]Man or GDP-[3H]Man. Double label analysis of proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicates that labeling of proteins with apparent molecular weights of 91,000 and 57,000 is enhanced upon diethylstilbestrol treatment. The effect is reversed upon hormone withdrawal. Analysis of [14C]Man-proteins by SDS-PAGE and fluorography reveals at least 18 labeled proteins, confirms the double label gel results, and shows changes in three additional proteins. Estrogen-induced changes in membrane proteins and glycoproteins were also studied by Coomassie blue staining, periodic acid-Schiff staining, and autoradiography of 125I-concanavalin A binding following SDS-PAGE. Changes are observed in at least 9 Coomassie blue, 7 periodic acid-Schiff and 13 125I-concanavalin A bands. Some proteins are enhanced by estrogen treatment and are diminished upon withdrawal, whereas other proteins are diminished by estrogen treatment and are enhanced by withdrawal. Hen oviduct membrane preparations were labeled with combinations of UDP-GlcNAc, GDP-Man, and UDP-Glc. Tunicamycin inhibits incorporation of [3H]Man and [14C]GlcNAc into protein by 84% and 65%, respectively. SDS-PAGE reveals that transfer of GlcNAc to a 36,000 protein is the least sensitive to tunicamycin. SDS-PAGE of proteins labeled by incubation with GDP-[14C]Man and UDP-[3H]Glc indicate that the presence of Glc does not alter the typical Man-protein-labeling pattern and processing of Glc from individual acceptors uniform. | [
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10,001,683 | Ribosomal protein synthesis during spore germination and vegetative growth in Dictyostelium discoideum. | [
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10,001,684 | We have investigated the regulation of the synthesis of each ribosomal protein during spore germination and in vegetatively growing cells of Dictyostelium discoideum. Germinating spores and exponentially growing amoebae were labeled with [35S]methionine and the individual ribosomal proteins were resolved by electrophoresis on two-dimensional polyacrylamide gels. Functional mRNA levels for ribosomal proteins were analyzed by translating the slime mold RNA in a wheat germ cell-free system and separating the translation products by two-dimensional polyacrylamide gel electrophoresis. All slime mold ribosomal proteins were synthesized at every stage of spore germination and during vegetative growth; and the amount of synthesis of these proteins was highest at 1 to 2 h of germination. A comparison between the synthesis of ribosomal proteins in germinating spores with those in vegetatively growing cells suggests that although the majority of them seem to be similarly regulated, the regulation of the synthesis of some of the proteins may be different in both conditions. Although dormant spores lacked functional mRNAs for 16 ribosomal proteins, these mRNAs were detected as early as 1 h of germination. The mRNAs corresponding to all ribosomal proteins were present throughout germination, and their levels were highest at 2 h of germination. These results are consistent with the conclusion that the synthesis of the majority of ribosomal proteins during spore germination is regulated transcriptionally. However, vegetative amoebae lack three proteins (A, E, and L in our nomenclature), yet contain mRNA for these proteins that can be translated in vitro. Therefore, the synthesis of these proteins is apparently regulated translationally. | [
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10,001,685 | Purification and ATP substrate site of a cyclic nucleotide-independent protein kinase (NI) from porcine liver nuclei. | [
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10,001,686 | Synthesis and inactivation of bacterial luciferase determined by immunochemical techniques. Comparison with total protein synthesis and turnover. | [
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10,001,687 | Podophyllotoxin-resistant mutants of Chinese hamster ovary cells. Alteration in a microtubule-associated protein. | [
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10,001,688 | Characterization of a human intestinal difucosyl heptaglycosylceramide with a blood group B determinant and a type 1 carbohydrate chain. | [
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10,001,689 | Kinetics and mechanism of carbon monoxide binding to purified liver microsomal cytochrome P-450 isozymes. | [
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10,001,690 | Haloenol lactones: enzyme-activated irreversible inactivators for serine proteases. Inactivation of alpha-chymotrypsin. | [
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10,001,691 | Two haloenol lactones have been shown to inactivate alpha-chymotrypsin by an enzyme-mediated process. According to the postulated mechanism, acylation of the active site serine by the haloenol lactone reveals an alpha-haloketone, which alkylates the enzyme at the active site and renders the enzyme inactive. The inactivation has been shown to require enzymatic activation of the inhibitor to its reactive form to take place within the acyl-enzyme intermediate and to be irreversible. | [
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] |
10,001,692 | Inhibition of chemotaxis by S-3-deazaadenosylhomocysteine in a mouse macrophage cell line. | [
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10,001,693 | Chemotaxis by a macrophage cell line, RAW264, is specifically inhibited by the intracellular accumulation of 3-deazaadenosylhomocysteine (3-deaza-AdoHcy). Intracellular accumulation of 3-deaza-AdoHcy is the result of incubation of the cells with 3-deazaadenosine, a compound that can function both as a substrate and as an inhibitor of adenosylhomocysteine (AdoHcy) hydrolase. Accumulation of AdoHcy per se, brought about in chemotactic cell lines by incubation with either 3-deazaadenosine or with 3-deazaaristeromycin, does not affect chemotaxis. The specific role of 3-deazaadenosine as an inhibitor of macrophage cell line chemotaxis is supported by the following findings. Another macrophage cell line, RAW309CR, is resistant to the inhibition of chemotaxis by 3-deazaadenosine, and the resistance can be ascribed to the failure to accumulate 3-deaza-AdoHcy in this cell line. It is noteworthy that both RAW264 and RAW309CR accumulate similar amounts of AdoHcy after incubation with 3-deazaadenosine. The difference in the accumulation of 3-deaza-AdoHcy in RAW264 and RAW309CR is explained by the finding that sonicates of RAW264 rapidly form 3-deaza-AdoHcy but sonicates of RAW309CR do not. Both hydrolysis of AdoHcy and the inhibition of the hydrolysis of AdoHcy by 3-deazaadenosine are the same in sonicates of the two macrophage cell lines. | [
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10,001,694 | Amino acid sequences of the epsilon and alpha E globins of HbE, a minor early embryonic hemoglobin of the chicken. | [
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] |
10,001,695 | We have determined amino acid sequences for the alpha-like and beta-like globin components of HbE, one of the two minor hemoglobins in early chick embryos. The complete primary structure of the epsilon chain differs at 18 positions from the adult chicken beta globin, but there are no changes in heme-binding residues, alpha 1 beta 2 contact positions, or allosteric regulatory sites. By amino acid sequence analysis, we have identified a new alpha-like globin that we have called alpha E. The alpha E globin chain differs from the major adult alpha A chain at 22 amino acid positions. This paper discusses the structural and implied functional characteristics of these globins and presents hypotheses regarding the possible role of minor embryonic hemoglobins. | [
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10,001,696 | Minor early embryonic chick hemoglobin M. Amino acid sequences of the epsilon and alpha D chains. | [
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10,001,697 | Erythrocytes of the early chick embryo contain four hemoglobins, two major and two minor. In this paper, we present amino acid sequences for the beta-like and alpha-like chains of HbM, the least abundant of the four early chick hemoglobins. The complete amino acid sequence of the beta-like chain of HbM is identical with that of the epsilon chain of HbE, the other minor early embryonic hemoglobin in the domestic chicken. Analysis of the alpha-like chain of HbM (92 of 141 residues) reveals a globin sequence closely related to the minor adult alpha D chain. Comparison of our sequence data with the nucleotide sequence of the alpha D globin gene suggests that a single gene encodes both the embryonic and adult alpha D globin polypeptides. We discuss the structure, possible function, and evolution of the HbM globin chains. | [
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10,001,698 | Clofibric acid and phenylpyruvic acid as biochemical probes for studying soluble bovine liver branched chain ketoacid dehydrogenase. | [
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10,001,699 | Purified bovine liver branched chain ketoacid dehydrogenase is inhibited by its reaction products, NADH and the branched chain acyl-CoA thiol esters. This provides an immediate, short term regulation mechanism for this enzyme which commits leucine, isoleucine, and valine to the catabolic pathway. We find that clofibric acid (p-chlorophenoxymethylpropionic acid) and phenylpyruvate also specifically inhibit this multienzyme complex. Fifty percent inhibition occurs at 0.24 mM and 0.40 mM concentrations, respectively. Both compounds bind either free enzyme (E) or the enzyme-substrate (ES) complex to give an abortive enzyme-substrate-inhibitor (ESI) complex. Phenylpyruvate preferentially binds E (Ki = 48 microM) over ES (alpha Ki = 97 microM), while clofibric acid has a reverse preference, binding ES (alpha Ki = 48 microM) with more avidity than E (Ki = 142 microM). Amino acids like L-leucine, beta-leucine, and 2-aminonorbornyl-2-carboxylic acid do not inhibit this enzyme at concentrations as high as 10 mM. These biochemical probes will be useful for studying the catalytic site of this enzyme complex and the consequences of accumulated branched chain ketoacids and their amino acid precursors on muscle and liver metabolism when the dehydrogenase function is impaired. | [
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