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10,003,700 | Severe hypertension was seen in a patient with mitral stenosis when in sinus rhythm. A large fall in systolic blood pressure and in left ventricular end-diastolic pressure was caused by ventricular pacing. Contrary to the usual situation in mitral stenosis this patient paradoxically is a subject who improved haemodynamically with the loss of sinus rhythm and of atrial systole. | [
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10,003,701 | Effects of halothane and sodium nitroprusside on renal function and autoregulation. | [
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10,003,702 | The effects of sodium nitroprusside and halothane on renal autoregulation and kidney function were studied in 14 mongrel dogs at mean arterial pressures of 120, 100, 80, 60 and 40 mm Hg. In group 1, stepwise decreases in mean arterial pressure were achieved by increasing the halothane concentration. In group II, mean arterial pressure was decreased by infusing sodium nitroprusside during halothane anaesthesia. In group I, renal blood flow decreased significantly at mean arterial pressures of 100, 80, 60 and 40 mm Hg. In group II, renal blood flow was well maintained at mean arterial pressures of 100 and 80 mm Hg, but decreased significantly at 60 and 40 mm Hg; at these low pressures flow was greater in group II than in group I. There were no significant differences between two groups in inulin clearance, inulin clearance/renal plasma flow, urine output, urine osmolarity and sodium excretion rate. Significantly larger fractions of cardiac output were distributed to the kidneys in group I. | [
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10,003,703 | Malposition of a central venous catheter. | [
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10,003,705 | Continuous suxamethonium infusion for microlaryngeal surgery. | [
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] |
10,003,706 | The suxamethonium infusion requirements to maintain a 100% neuromuscular block in patients undergoing microlaryngeal surgery were determined. Anaesthesia was maintained with halothane and the neuromuscular block monitored with a peripheral nerve stimulator delivering train-of-four stimuli. Suxamethonium requirements varied directly with time in a linear manner and the onset and recovery time of the block increased with time. No difficulties in recovery were observed when the duration of infusion was less than l h, even when the nature of the block had altered to the non-depolarizing type. A good correlation was observed between neuromuscular block as indicated by peripheral nerve stimulation and laryngeal muscle paralysis. | [
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10,003,707 | Antagonism of ketamine by 4-aminopyridine and physostigmine. | [
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] |
10,003,708 | Remote monitoring by mass spectrometry during anaesthesia. Evaluation of a suitable inlet system. | [
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10,003,709 | We describe a three-stage mass spectrometer inlet system suitable for use in operating theatres and evidence of its performance in delay and response times to step changes in oxygen and halothane concentrations. At 55 m and a sampled gas flow of 100mlmin-1, the inlet imposed a delay of 21s and prolonged the 10-90% response to 310ms for oxygen and 510ms for halothane. A linear relationship between inlet length and 10-90% response time at constant sampled gas flow was demonstrated for halothane but not for oxygen. Our results compared with those of other workers support the chosen compromise between practical flexibility and convenience versus maximum speed of response that was adopted in this system design. | [
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10,003,710 | Influence of extradural morphine on the adrenocortical and hyperglycaemic response to surgery. | [
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10,003,711 | Plasma cortisol and glucose concentrations were measured repeatedly from before the induction of anaesthesia until 9 h after skin incision in 36 patients undergoing abdominal hysterectomy. Twelve patients received general anaesthesia (halothane), and systemic opiates for postoperative pain; a further 12 patients underwent continuous extradural analgesia with a local anaesthetic agent (bupivacaine) and in the remaining 12 patients general anaesthesia (halothane) plus extradural morphine (4mg before skin incision and an additional 4mg at skin closure) were used. Patients receiving extradural morphine or bupivacaine were free of pain. The physiological cortisol and glucose response to surgery was blocked by the extradural analgesia with bupivacaine. Extradural morphine did not modify the initial increase in plasma cortisol and glucose concentrations during surgery, but suppressed the hyperglycaemic and cortisol response following surgery when compared with the general anaesthesia group receiving systemic opiates. However, cortisol and glucose concentrations were greater after operation in patients receiving extradural morphine compared with extradural bupivacaine, suggesting that the endocrine metabolic response to surgery is predominantly released by neurogenic stimuli other than pain stimuli involving opiate receptor-dependent nociceptive pathways. | [
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10,003,712 | Suppression of antidiuretic hormone hypersecretion during surgery by extradural anaesthesia. | [
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10,003,713 | The concentrations of antidiuretic hormone in plasma and urine were determined in three groups of patients submitted to the same operative procedure. Seven (group I) underwent general anaesthesia with thiopentone, fentanyl and nitrous oxide and received an infusion of isotonic saline solution 5ml min-l. Seven patients (group II) anaesthetized similarly, received isotonic saline solution 15 ml min-1. In group III (five patients) anaesthesia was produced by extradural blockade. Surgery under general anaesthesia induced a significant increase in plasma and urine ADH concentrations which were not modified by the fluid load. Extradural anaesthesia suppressed almost completely the release of ADH during surgery. This effect of extradural anaesthesia could be related to the interruption of conduction along nociceptive neural pathways. | [
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10,003,714 | Comparison of i. m. diazepam and hydroxyzine as premedicants. | [
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10,003,715 | The effectiveness of diazepam as premedication when administered by two different i.m. techniques was compared with hydroxyzine in 84 male and 101 female patients. Variables studied were relief of anxiety, sedation, acceptance by both patient and anaesthetist and pain at the injection site. Diazepam 20 mg in male and diazepam 10 mg in female patients administered by a Z-track injection technique were as effective premedicants as hydroxyzine 100mg i.m. In men diazepam was good as hydroxyzine regardless of the injection technique. In women the technique of injection of the diazepam was critical to achieving good results. The Z-track injection method decreased the amount of severe pain on injection in both sexes to levels similar to those achieved with hydroxyzine. | [
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10,003,716 | Single-dose comparison of buprenorphine 0.3 and 0.6 mg i.v. given after operation: clinical effects and plasma concentration. | [
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10,003,717 | The plasma concentrations and clinical effects of a single i.v. dose of buprenorphine 0.3 or 0.6 mg were studied in patients recovering from surgery. Analgesic and hormonal effects were greater with the greater dose without a parallel increase in respiratory depression. A comparison with previous work suggests that increased efficacy results either from the use of the larger dose or equivalently if the first required postoperative dose of 0.3 mg has been preceded by a similar loading dose. | [
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10,003,718 | Effects of the inhalation of enflurane on hepatic microsomal enzymatic activities in the rat. | [
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10,003,719 | We have investigated the possible hepatic microsomal enzyme induction effect of enflurane, administered by inhalation in anaesthetic doses for periods of 1 h a day. In 15 experimental groups, differing from one another in anaesthetics concentration (1, 2 and 3% enflurane) and in the number of days of treatment (3, 7, 10, 14, 21 and 24) we found (1) no alteration either in the ratio of liver to body weight or in the microsomal protein content; (2) no modifications in aniline hydroxylase activity; (3) a decrease in the aminopyrine demethylase activity after a number of exposures, varying with the dose. | [
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10,003,720 | The IgG binding function of the normal red cell plasma membrane: identification of integral polypeptides that bind IgG. | [
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] |
10,003,721 | Non-immune IgG binds to red cell integral membrane proteins obtained by mild alkaline extraction of ghosts. Detergent gel chromatography and electrophoretic analyses of IgG-membrane protein complexes obtained by nonionic detergent solubilization and affinity binding to protein A indicate that three polypeptides participate in the binding of IgG. These have apparent molecular weights of 90 000, 44 000 and 22 000 and are present in a 1:3:0.9 stoichiometry. Evidence obtained indicates that the major sialoglycoproteins are not involved in this type of binding. | [
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10,003,722 | Comparison of antithrombin III assays using biological and chromogenic substrates. | [
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10,003,723 | Antithrombin III (At III) levels in plasma samples were determined by incubation of diluted plasma with thrombin, either with or without heparin. Followed by measurement of residual thrombin using clotting and amidolytic methods. All assays were of multidose bioassay design suitable for parallel line analysis. Assays without heparin showed only a small difference between amidolytic and clotting methods, which was not significant at the 95% level. Assays with heparin showed a much larger difference between clotting and amidolytic methods which is shown to be attributable to the heat defibrination step used in the clotting assay. Heat defibrination and ancrod defibrination methods are compared using the amidolytic heparin cofactor assay and it is shown that heat defibrination can cause the loss of nearly 50% of the functional At III in a reconstituted freeze-dried plasma which was used as a standard. No loss occurs when ancrod is used for defibrination. It is an advantage of the amidolytic heparin cofactor assay that defibrination is not required. | [
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10,003,724 | Polymorphonuclear leucocyte hypersegmentation in heat stroke. | [
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10,003,725 | Ristocetin-induced platelet agglutination in Afro-Caribbean and Caucasian people. | [
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10,003,726 | Endotoxin in paroxysmal nocturnal haemoglobulinuria (PNH) | [
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10,003,727 | Erythrocytes with different surface immunological properties in the peripheral blood of beta-thalassaemic patients. | [
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10,003,728 | Erythrocyte membrane proteins in an unusual case of congenital dyserythropoietic anaemia type II (CDA II). | [
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10,003,729 | Analysis of the erythrocyte membrane protein of an atypical case of congenital dyserythropoietic anaemia type II (CDA II) by electrophoresis on polyacrylamide gels revealed marked abnormalities. One-dimensional analysis showed a pronounced decrease in levels of B1.1 components, an increase in the level of B1.4 and the appearance of new components in the A region as well as in the C region of the gel. There were no artefacts due either to the presence of early red cells or to abnormally high levels of proteolytic enzyme activity in the CDA II preparations. Two-dimensional analysis confirmed the alterations in membrane components showing two novel A region species not reported in other studies of this disease. Abnormalities in components of such large molecular size may explain the greater degree of membrane perturbation seen in the present case and support the idea that CDA II may embrace more than one disease entity. | [
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10,003,731 | Using urea-polyacrylamide gel electrophoresis it has been possible to distinguish the molecular forms of transferrin in rabbit serum. When 59Fe-labelled diferric transferrin is injected into normal, anaemic or hypertransfused, polycythaemic rabbits, iron is removed from diferric transferrin in essentially pairwise fashion. Exchange of iron between transferrin and tissues was also studied using predominantly monoferric transferrin labelled with 59Fe or 125I, and with 125I-labelled apotransferrin. The return of iron from tissue stores to circulating transferrin occurs one atom at a time to either site of the protein and, possibly, in pairwise fashion as well. The rate of clearance of iron from diferric transferrin differs from that of monoferric transferrins, and the rates at which iron is returned to empty sites of transferrin also differ, so that serum iron is not a kinetically homogeneous pool in the rabbit. | [
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10,003,732 | A new perspective on Bruch's membrane and the retinal pigment epithelium. | [
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10,003,733 | Trypsin digestion of retinal pigment epithelium is a technique that bares Bruch's membrane, to allow topographical examination by scanning electron microscopy. Twenty-five human eyes were examined. The zonula occludens of the pigment epithelium was clearly seen as a surface feature, but attachment plaques at the sides and base were not visible. The adhesion between the pigment epithelium and the basal lamina was stronger than between the basal lamina and the rest of Bruch's membrane. Surface features of the basal lamina, inner collagenous zone, elastic layer, and outer collagenous zone were seen in a way that previously required an artist's representation constructed from microscopic sections. | [
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] |
10,003,734 | Preserved para-arteriole retinal pigment epithelium (PPRPE) in retinitis pigmentosa. | [
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10,003,735 | Five patients with retinitis pigmentosa (RP) with probable autosomal recessive inheritance have been identified in whom there is relative preservation of retinal pigment epithelium adjacent to and under retinal arterioles despite a panretinal degenerative process. All the patients were hypermetropic, though patients with RP tend to be myopic. This implies that there is a factor associated with retinal arterioles which locally retards the RP process in these patients. It may be appropriate to look for the PPRPE pattern in hypermetropic RP patients. | [
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] |
10,003,736 | The age variation of "senile' cataract in various parts of the world. | [
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] |
10,003,737 | As its name implies, senile cataract is age-related. A survey of 9 studies indicates to what extent the epidemiology of senile cataract can be represented by Gompertz plots. These are shown to be suitable separately for men and women and to enable one to distinguish between inborn and environmental influences. Further support is obtained for the view that myopia and cataract may be linked, and attention is drawn to the potentially noxious effect of (ultraviolet) light. | [
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10,003,738 | Prevalence and aetiology of cataract in Punjab. | [
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10,003,739 | Three districts in the Punjab plains were surveyed in 1976-7 for senile cataract and potential risk factors. The prevalence of senile cataract was 15.3% among 1269 persons examined who were aged 30 ang older, and 4.3% for all ages. These figures confirmed previous reports of high prevalence. The prevalence was 1% for ages 30-49 and increased markedly in the sixth and seventh decades to 67% for ages 70 and older. Univariate age-adjusted analyses of selected socioeconomic, demographic, dietary, and other variables suggested that a higher prevalence was associated with being widowed, low education, use of rock salt in cooking, infrequent consumption of various protein foods (beans and lentils, milk, eggs, and curd), short height, and low weight. Multivariate analysis further suggested low total protein consumption as a risk factor that may account for as much as 40% of the excess prevalence of Punjab cataract over that in a US population study. | [
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10,003,740 | Expulsive choroidal haemorrhage: an experimental study. | [
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10,003,741 | The closure of 2 or more vortex veins in rabbits' eyes results in a persistent rise in intraocular pressure. In these circumstances a small opening into the anterior chamber at the corneoscleral limbus resulted in an iris prolapse, while a larger limbal wound was immediately followed by a massive choroidal expulsive haemorrhage. It appears from this experimental study that a definite and persistent rise in intraocular pressure could be a sign of impaired venous outflow and that the occurrence of an iris prolapse after small limbal opening into the anterior chamber should prompt us to examine the fundus of the eye and look for a choroidal complication. It is possible that the present set of experiments is relevant to the mechanism of expulsive haemorrhage in man. | [
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] |
10,003,742 | Visual disorders associated with cerebral palsy. | [
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10,003,743 | School children severely afflicted with cerebral palsy, but unselected in regard to their visual status. have been studied. Of 120 children examined only 24 (20%) had normal eyes or ocular adnexae. Squint was found in 52.5% of the children and significant refractive errors in 50%. There was also a high incidence of strabismic and anisometropic amblyopia (15%) and visual field defects (11%). A number of other ocular abnormalities were found, the majority of which were not amenable to any form of treatment. Early identification of treatable ocular defects and their treatment along conventional lines is emphasised. It is also important to identify untreatable defects that may have a bearing on the child's education. These children need all the help available, and visual function should be at its best. A diagnosis of cerebral palsy has usually been made by the time such a child reaches 18 months of age. In view of the high incidence of ocular defects a full ophthalmological assessment should be part of the routine assessment of the child. | [
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10,003,744 | Intraocular lens power calculation for emmetropia: a clinical study. | [
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10,003,745 | A series of 50 eyes received an intraocular lens (IOL) of power calculated for emmetropia from data of axial length, corneal curvature, and postoperative anterior chamber depth by R. D. Binkhorst's formulae. The postoperative refraction results were compared with those of 100 control eyes which received +19 D standard power IOLs without calculation. The calculated group had postoperative refractions which were closer to emmetropia, and the difference was os statistical significance, with 92% within the +/- 1D range and 98% within the +/- 2 D range from emmetropia. The calculated predictions of postoperative refraction were of a useful level of accuracy. Consideration of the sources of error indicates that there is no justification for the use of IOLs in power steps of less than 1 D. The calculation of IOL power allows the surgeon to control the postoperative refraction and avoid unwanted ametropia. | [
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10,003,746 | Binocular interactions in normal and anomalous binocular vision: effects of flicker. | [
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10,003,747 | Temporal modulation thresholds were determined for monocular viewing and for binocular viewing of stimuli presented in phase or in counterphase to each eye of observers with normal binocular vision and those lacking stereopsis. The results showed that in individuals with normal binocular vision sensitivity was much greater for in-phase than for counterphase stimulation at low temporal frequencies, but that this superiority declined at higher temporal frequencies. Averaged across frequencies, binocular sensitivity for in-phase stimulation was 40-50% higher than monocular sensitivity. In contrast, in the observers lacking stereopsis the ratios of binocular in-phase/monocular sensitivity averaged 1.02, and there were no significant differences in sensitivity to in-phase and counterphase stimulation. This failure of binocular integration at threshold does not result from differences in transmission time between the 2 eyes. However, while individuals lacking stereopsis showed an absence of binocular interaction for uniform-field flicker at threshold, they showed suprathreshold dichoptic temporal frequency masking which was similar to that found in normal persons. | [
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10,003,748 | The sensitive period: transfer of fixation after occlusion for strabismic amblyopia. | [
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10,003,749 | Of 2649 patients with strabismic amblyopia who attended clinics between 1941 and 1978, 1904 patients were reviewed. Of these, 115 patients had transfer of fixation after occlusion. These were analysed according to the type of transfer of fixation and relation to occlusion and age. The period of maximum sensitivity to short periods of occlusion extended to 18 months, declining to about 30 months of age. Sensitivity to occlusion reaches low levels about the age of 5 to 51/2 years, though it is still present to some degree, especially in patients with relatively good visual acuity of 6/36 or better. The upper limit of the sensitive period was found to be about the end of the seventh year of age. | [
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] |
10,003,750 | Lacrimal canalicular obstruction following chickenpox. | [
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10,003,751 | Parallel measurements of bound calcium and force in glycerinated rabbit psoas muscle fibers. | [
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10,003,752 | A simple double-isotope procedure has been developed for making simultaneous measurements of bound Ca2+ and relative force in glycerinated rabbit psoas bundles containing two fibers. With this preparation it is possible to study Ca2+-troponin interactions coincident with MgATP-induced force development. Over the free [Ca2+] range 6 . 10(-8)--1.2 . 10(-5) M the bound Ca2+ varied from 0.25 to 1.65 mumol/g protein. The free [Ca2+] at half-maximal Ca2+ saturation was 2 . 10(-7) M while that a half-maximal force was 5 . 10(-7) M. Half-maximal Ca2+ saturation was associated with 20% maximal force. The force-[Ca2+] saturation curve showed a steep rise in slope at greater than half saturation. The observed relationship was consistent with a model in which multiple occupancy of troponin Ca2+-binding sites is essential for initiation of cross-bridge cycling. | [
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10,003,753 | Transport of Na+ by monensin across bimolecular lipid membranes. | [
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10,003,754 | Transmembrane 22Na fluxes across bimolecular lipid membranes are measured under two different experimental conditions: (a) the pH is the same in the two bulk aqueous solutions on either side of the membrane while the concentrations of Na+ are different; (b) the concentration of Na+ are identical but pH of the two solutions are different. In this latter case, the transport of Na+ occurs in the opposite direction to the difference of the proton concentration. In both cases, the electrical charge flux in negligible. A transport model is proposed to account for the experimental data. | [
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10,003,755 | Sodium uptake mechanisms in brush-border membrane vesicles prepared from rabbit renal cortex. | [
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10,003,756 | Transport of glucosamine (aldohexoses) by preimplantation mouse blastocysts. | [
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10,003,757 | A carrier-mediated system for uptake of glucosamine (and other aldohexoses) was identified in preimplantation mouse blastocysts. With early and late blastocysts, the values for Km (525 +/- 82 and 651 +/- 137 microM, respectively) and V (0.29 +/- 0.02 and 0.29 +/- 0.04 pmol/h per ng protein, respectively) for glucosamine are statistically indistinguishable. The same carrier system, therefore, is operative at the two developmental stages. Glucosamine uptake was temperature sensitive and inhibited by structural analogues and phloretin. | [
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10,003,758 | Stabilization of bilayer structure for unsaturated phosphatidylethanolamines by detergents. | [
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10,003,759 | The structural preferences of mixed lipid systems containing egg yolk or 18:1c/18:1c phosphatidylethanolamine and representative detergents (Triton X-100, deoxycholate, octylglucoside and lyso-phosphatidylcholine) have been examined. It is shown that all these detergents exhibit an ability to stabilize a bilayer organization for the phosphatidylethanolamine at detergent to phosphatidylethanolamine molar rations of 0.05 to 0.5, depending on the detergent and/or phosphatidylethanolamine species. These results are interpreted in terms of molecular shape, where the 'inverted cone' shape detergents combine in a complementary fashion with 'cone shaped' phosphatidylethanolamine to result in net bilayer structure. | [
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10,003,760 | Transport of ascorbate into guinea pig liver mitochondria. | [
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10,003,761 | The amount of ascorbate associated with guinea pig liver mitochondria was estimated by high-performance liquid chromatography. Incubation of mitochondria with ascorbate revealed a time-dependent and temperature-dependent accumulation of the vitamin. A steady-state level of ascorbate was obtained in the mitochondria after about 20 min of incubation at 37 degrees C, whereas no accumulation was observed at 0 degrees C. The matrix concentration of ascorbate was highly correlated to the concentration of ascorbate in the incubation medium. The initial rate of accumulation (about 7 pmol/mg protein per min at 10 degrees C) was three orders of magnitude less than for compounds that are transported across the mitochondrial inner membrane by specific carriers. Experiments with the enzyme ascorbate oxidase demonstrated that the mitochondrial membrane is also permeable to dehydroascorbate, and that the accumulated dehydroascorbate is stable in the mitochondria. There was no effect of the energy state of the mitochondrial membrane of the initial transport rate of ascorbate. Electrostatic binding of ascorbate to the membrane was excluded from experiments performed in isosmotic potassium chloride medium. Diffusion of ascorbate across the mitochondrial inner membrane accounts for the experimental findings. | [
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10,003,762 | Core structure, internal osmotic pressure and irreversible structural changes of chromaffin granules during osmometer behaviour. | [
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] |
10,003,763 | In the adrenal medullary cells, catecholamines are stored in and secreted from specialized secretory vesicles, the chromaffin granules. In order to gain some understanding of both functions of chromaffin granules, it is important to characterize their biophysical organization. Using isolated bovine chromaffin granules we have investigated the osmometer behaviour of chromaffin granules by 31P-NMR and fluorescence spectroscopy, by turbidity measurements and by electron-microscopic determination of chromaffin granule size distributions. On the basis of the osmometer model we have formulated equations predicting the behaviour of the native catecholamine fluorescence quenching and of the size of chromaffin granules a a function of osmolarity and have shown experimentally that the granules' behaviour conforms to these. It was possible to estimate the osmotic activity of the chromaffin granule core solution and the mean absolute water space in chromaffin granules from the determination of the size distributions as a function of osmotic pressure. With NMR spectroscopy a selective line-broadening of the alpha-resonances was observed with increasing osmolarities, while the gamma-phosphorus resonances remained virtually unchanged. Possibly there is an increase in core viscosity with osmolarity which affects only the alpha- and beta-phosphorus groups. While suspending chromaffin granules from lower to higher osmolarities causes no lysis, moving them back to their original osmolarity at which they were previously stable lyses them, thereby releasing a maximum of 70% of their releasable protein. This 'hyperosmolar' lysis is independent of preincubation times in the higher osmolarities and of the absolute dilution applied but depends on dilution beyond the 405 to 322 mosM sucrose range. Under the experiment conditions no uptake of sucrose from the medium into the granules could be measured, thereby suggesting that hyperosmolar lysis is a phenomenon not due to solute penetration. Since with NMR and fluorescence spectroscopy no chemical changes in the core composition can be observed, we conclude that hyperosmolar lysis may be caused by irreversible membrane relaxation upon osmotic shrinking. | [
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10,003,764 | 31P-NMR studies on phospholipid structure in membranes of intact, functionally-active, rat liver mitochondria. | [
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10,003,765 | The structure of mixed cholesterol-phospholipid monolayers spread at the air-water interface as probed by interactions with band 3 protein from erythrocyte membranes. | [
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10,003,766 | Solubilized band 3 protein from human erythrocyte membranes (the anion transport protein) interacts strongly and specifically with monolayers of cholesterol spread at the air-water interface whereas, at pH 7-10, it shows only moderate interactions with phospholipid monolayers (Klappauf, E. and Schubert, D. (1979) Hoppe-Seyler's Z. Physiol. Chem. 360, 1225-1235). When band 3 protein, at pH 7 and an ionic strength of approx. 100 mM, is added to the subphase of mixed cholesterol-glycerophospholipid monolayers, the changes delta pi in monolayer surface pressure induced by the protein depend on the mole fraction X of sterol in the mixture. However, delta pi (X) only increases with increasing X towards the high values of delta pi that are characteristic of cholesterol monolayers if X greater than 0.67 +/- 0.04; at lower cholesterol content, delta pi (X) is practically identical to the value obtained with the pure glycerophospholipid. With mixtures of coprostanol and glycerophospholipids, the break in the delta pi (X) curves occurs when X = 0.33 +/- 0.03. Cholesterol-sphingomyelin and epicoprostanol-phosphatidylethanolamine mixtures show an increase of delta pi (X) when X greater than 0. The data seem to support earlier claims that cholesterol can form stoichiometric complexes with glycerophospholipids, the stoichiometries revealed by the band 3-monolayer interactions being 2:1 and 1:2. They also show that cholesterol-sphingomyelin complexes, if they should exist, must be structurally different from the cholesterol-glycerophospholipid complexes. | [
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10,003,767 | Interactions of chlorinated hydrocarbon insecticides with membranes. | [
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10,003,768 | Chlorinated hydrocarbon insecticides quench the fluorescence of N-alkyl derivatives of carbazole. We used phospholipids with covalently attached carbazole as probes for the interactions of chlorinated hydrocarbon insecticides with lipid bilayers, the object being to understand better the toxicities of chlorinated hydro-carbons. Fluorescence quenching measurements revealed the lipid-water partition coefficients of the chlorinated hydrocarbons, their diffusion coefficients in the membranes, and the binding capacities of the membranes for the chlorinated hydrocarbons. Active insecticides were compared with inactive analogues to test whether activities correlated with chlorinated hydrocarbon-membrane interactions. Thus DDT and methoxychlor were compared with inactive DDE, and insecticidal gamma-lindane was compared with three less active stereoisomers. The partition coefficients, diffusion coefficients and membrane saturation capacities did not correlate with insecticidal potency. The partition coefficients of these chlorinated hydrocarbons were larger in bilayers containing unsaturated fatty acyl chains as compared to bilayers containing saturated fatty acyl chains. Interestingly, neural membranes are known to contain a large percentage of unsaturated lipids. Our results indicate that the activities of chlorinated hydrocarbons are not a result of specific interactions of these compounds with the lipids of membranes. However, the neurotoxicity of chlorinated hydrocarbons may be amplified by selective partitioning in the unsaturated neural membranes. | [
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10,003,769 | Discrimination of three parallel pathways of lactate transport in the human erythrocyte membrane by inhibitors and kinetic properties. | [
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10,003,770 | The transmembrane movements of lactate and other monocarboxylate anions in mammalian erythrocytes have been claimed, by virtue of their sensitivity to SH-reagents, to involve a transfer system different from the classical anion system (Deuticke, B., Rickert, I. and Beyer, E. (1978) Biochim. Biophys. Acta 507, 137-155). Inhibition of monocarboxylate transfer by SH-reagents, however, was incomplete to an extent varying for different monocarboxylates. The transport component insensitive to SH-reagents has now been shown to involve (a) the classical anion-exchange system, as demonstrated by sensitivity to specific disulfonate inhibitors, and (b) nonionic diffusion, as indicated by the characteristic pH- and concentration dependency of this component and its stimulation by aliphatic alcohols. Under physiological conditions about 90% of total lactate movement proceed via the specific system, 5% via the classical anion-transfer system, 5% by nonionic diffusion. These three components of lactate exchange differ in their activation energies. The specific lactate system mediates net fluxes almost as fast as exchange fluxes, in marked contrast to the classical anion-exchange system which mediated halide exchange much faster than halide net movements. The underlying mechanism, for maintenance of electroneutrality, is an OH- -antiport or an H+ -symport as indicated by the particular response of lactate net fluxes to changes of intra- or extracellular pH. | [
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10,003,771 | Transport of neutral amino acids by human erythrocytes. | [
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10,003,772 | The transport of several neutral amino acids by human erythrocytes in vitro was studied. The measurements made included steady-state distributions, kinetics of initial rates of uptake, effects of monovalent cations and anions, general mutual inhibitory interactions, kinetics of inhibitions, effluxes, ability to produce accelerative exchange diffusion, and the inhibitory action of the thiol reagent N-ethylmaleimide. The results are interpreted as showing that the human erythrocyte membrane possesses several distinct transport systems for these amino acids, including one Na+-dependent system and one dependent on both Na+ and a suitable anion, that are qualitatively similar to those systems previously described in pigeon erythrocytes and mammalian reticulocytes. Quantitatively, however, the systems differ among the different kinds of red cell and a major difference lies in their abilities to produce accelerative exchange diffusion. | [
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10,003,773 | Local anesthetics and divalent cations have the same effect on the headgroups of phosphatidylcholine and phosphatidylethanolamine. | [
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] |
10,003,774 | The effects of the local anesthetic dibucaine on the membrane headgroup conformations of phosphatidylcholine and phosphatidylethanolamine were determined using 2H- and 31P-NMR. The size of the deuterium quadrupole splittings of the two methylene segments of the choline and ethanolamine groups changed dramatically and the 31-phosphorus chemical shift anisotropy of the phosphatidylcholine headgroup decreased by about 7 ppm in the presence of local anesthetic. The quadrupole splittings of the 3-glycerol and choline methyl segments were relatively insensitive to the addition of dibucaine. The headgroup data for dibucaine addition paralleled similar data for the addition of various cations. These NMR results agree with the previous observation that these drugs displace calcium from phospholipids. The effects of this local anesthetic on these headgroups were distinctly different from the changes induced by cholesterol, heat and the general anesthetic chloroform. | [
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10,003,775 | Proton permeability of liposomes from natural phospholipid mixtures. | [
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] |
10,003,776 | A method to determine the proton permeability coefficient of phospholipid membrane with the fluorescent probe pyranine is described. Very high proton permeability coefficients of liposomes from natural extracts are measured with great accuracy. The proton permeability appears to be linearly related to the fluidity of the bilayers. This relation as well as the comparison of the activation energies of proton permeability and fluidity support the hypothesis (Nichols J.W. and Deamer D.W. (1980) Proc. Natl. Acad. Sci. USA 77, 2038--2042) of a transfer process along a network of hydrogen bonded water molecules. It is suggested that the common lipid characteristics of biological membranes (net surface charge and unsaturation) favor a high proton permeability. | [
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10,003,777 | Specific binding sites for D-alpha-tocopherol on human erythrocytes. | [
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10,003,778 | Since vitamin E deficiency is associated with increased susceptibility of erythrocytes to hemolysis, we investigated the presence of tocopherol binding sites in human red blood cells. Erythrocytes were found to have specific binding sites for D-alpha-[3H]tocopherol with properties of receptors. Kinetic studies of binding demonstrated two binding sites: one with high affinity (equilibrium association constant Ka = 2.6 x 10(7) M-1), low capacity (7600 sites/cell) and the second with low affinity (Ka = 1.24 x 10(6) M-1), high capacity (150 000 sites/cell). These sites are at least partly protein in nature. | [
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10,003,779 | Action of peptidolipidic antibiotics of the iturin group on erythrocytes. Effect of some lipids on hemolysis. | [
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10,003,780 | Iturin A, bacillomycin L and bacillomycin L dimethyl ester have a strong lytic activity upon human erythrocytes while iturin C is totally inactive. The hemolytic action of the antibiotics is inhibited by free cholesterol as well as by cholesterol included in mixed liposomes of phosphatidylcholine-cholesterol and to a lesser extent by phosphatidylcholine liposomes. This inhibition is the result of an interaction between the antibiotic and added lipids which diminishes the concentration of free antibiotic available to lyse erythrocytes. The inhibitory effect of liposomes on hemolysis demonstrated the affinity of the antibiotic for artificial membrane, especially those containing cholesterol. | [
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10,003,781 | The effect of amine structure on complexation with lasalocid in model membrane systems. I. Identification of charged complexes in lipid bilayer membranes. | [
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10,003,782 | The electrical properties of X-537A (lasalocid) doped lipid bilayer membranes were studied in the presence of a series of nine biogenic amines which contain beta-phenylethylamine as the basic structural unit. The ionophore antibiotic was found to form charged complexes within the membrane during the transport of some of the amines. The dependence of membrane conductance on the concentration of ionophore and amine was studied. The amines are divided into three classes according to the nature of the complexes formed: (1) charged complex involving two ionophores (phenylephrine, metanephrine, and amphetamine); (2) charged complex containing three ionophores (dopamine, norepinephrine and epinephrine); and (3) no charged species formed (p- and m-tyramine and beta-phenylethylamine). | [
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10,003,783 | Cell surface of the fish pathogenic bacterium Aeromonas salmonicida. I. Relationship between autoagglutination and the presence of a major cell envelope protein. | [
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] |
10,003,784 | A comparison was made of membrane protein patterns of various Aeromonas salmonicida strains, initially isolated from different habitats with respect to fish species affected, pathological entity, and geographic location of the outbreak of the disease. A major protein with a molecular weight of 54 000 was found in all autoagglutinating strains, whereas this protein is present in low amounts, or not at all, in non-autoagglutinating strains. Evidence for a causal relationship between the presence of this protein and the phenomenon of autoagglutination came from the observation that a change of the growth medium led simultaneously to an almost complete loss of the additional cell envelope protein and the property of autoagglutination. As it has already been reported that autoagglutination is correlated with the presence of an additional cell surface layer, we hypothesize that the additional cell envelope protein is the (major) subunit of this layer. The application of the gel immuno radio assay, an immunological technique suited to detect antigens in a gel, revealed that the additional cell envelope proteins of all tested strains are immunologically related. The possibility to the use of this protein as a component of a vaccine against A. salmonicida infections is discussed. | [
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10,003,785 | Cell surface of the fish pathogenic bacterium Aeromonas salmonicida. II. Purification and characterization of a major cell envelope protein related to autoagglutination, adhesion and virulence. | [
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10,003,786 | The purification of the major protein of the membrane fraction of an autoagglutinating strain of Aeromonas salmonicida is described. This protein, designated as additional cell envelope protein, is water-insoluble, has a molecular weight of about 54 000 and its amino terminal sequence is H2N-Asp-Val-Leu-Leu. Neither sulphur-containing amino acids nor sugar residues were detected. Its amino acid composition, which shows that the additional cell envelope protein is hydrophobic in nature, is remarkably similar to those of various proteins known to be present in additional surface layers of other bacteria, to the adhesive K88 fimbriae of enteropathogenic Escherichia coli and to a pore protein of the outer membrane of E. coli K12. | [
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10,003,787 | Divalent cations and chlorpromazine can induce non-bilayer structures in phosphatidic acid-containing model membranes. | [
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10,003,788 | (1) The structural organization of aqueous dispersions of 1,2-dioleoylphosphatidic acid has been investigated by freeze-fracture electron microscopy in relation to variations in pH, divalent cations and the local anaesthetic chlorpromazine. (2) In the pH range 4--8 in the presence of 100 mM Na+, dioleoylphosphatidic acid is organized in bilayers. (3) At pH 6 and not at pH 4 and 8.5 addition of Ca2+, Mg2+, Mn2+ and chlorpromazine results in the formation of the hexagonal HII phase. (4) Ca2+ and chlorpromazine addition to mixed phosphatidylcholine-dioleoylphosphatidic acid bilayers at pH 6 results in the formation of lipidic particles. | [
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10,003,789 | Cross-linking studies of the protein topography of rat liver microsomes. | [
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10,003,790 | A cleavable cross-linking reagent, dithiobis(succinimidyl propionate), DSP, was used to study the topography of the proteins in the endoplasmic reticulum membrane of rat liver. Reaction of untreated (control), phenobarbital- or 3-methylcholanthrene-induced microsomes with 0.5 mM DSP for 30 min at 0 degrees C resulted in the cross-linking of a protein with a molecular weight of about 52 000 to form an apparent dimer. In phenobarbital microsomes, a smaller amount of a 52 000-dalton protein also appeared in a dimer in the absence of DSP if N-ethylmaleimide was not included during homogenization. In phenobarbital and 3-methylcholanthrene microsomes, a 48 000-dalton protein was cross-linked by DSP to a protein of about 57 000. In all three types of microsomes, a protein with an Mr of about 52 000 was also cross-linked to a protein of about 79 000. In phenobarbital and control microsomes, cross-linking resulted in an oligomeric protein of approximate molecular weight 180 000 which contained three proteins, two with Mr of about 52 000 and one about 79 000. Under the cross-linking conditions, little or no denaturation of cytochrome P-450 and NADPH-cytochrome c reductase was observed. The aryl hydrocarbon hydroxylase activity was significantly inhibited by the bifunctional cross-linking reagent, DSP, but not by the monofunctional reagent N-succinimidyl-3-(4-hydroxyphenyl) propionate. However, attempts to regenerate the aryl hydrocarbon hydroxylase activity by cleavage of the disulfide linkage with 2-mercaptoethanol or dithiothreitol were not successful. | [
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10,003,791 | Structural changes in alkaline-treated postsynaptic membranes from Torpedo marmorata are not due to lipid hydrolysis. | [
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10,003,792 | The removal of the 43 kDa peptide from postsynaptic membranes from the electric organ of Torpedo by alkaline treatment (pH 11) or by lithium diiodosalicylate at pH 8 results in similar ultrastructural changes that cannot be produced by the action of porcine pancreatic phospholipase A2 or lysophosphatidylcholine. Thin-layer chromatography fails to reveal significant alkaline hydrolysis of membrane lipids from postsynaptic membranes and erythrocyte ghosts under the conditions used for peptide extraction. | [
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10,003,793 | Effect of pH on the transport of Krebs cycle intermediates in renal brush border membranes. | [
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10,003,794 | Lowering extravesicular pH stimulated Na+-dependent citrate transport in renal brush border membrane vesicles: e.g., at pHout = 5.5, the initial rate of citrate uptake was increased 10-fold compared to parallel control experiments at pH 7.5. The same experimental conditions had little effect on succinate uptake. The influence of pH on citrate transport is a product of the extravesicular H+ concentration; pH gradients did not potentiate the effects nor were proton gradients capable of driving transport in the absence of Na+. The effect of pH is adequately explained if only the mono- and divalent species of citrate (Cit1-, Cit2-) are considered acceptable substrates for transport. The stimulatory influence of pH on transport correlated quite well with pH-related increases in the concentrations of Cit1- and Cit2-, and over the same pH range [Cit3-] was inversely related to citrate uptake. A model of the Na+-dependent dicarboxylate transport system is discussed in which three sodium ions are translocated per molecule of dicarboxylic acid. | [
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10,003,795 | The effect of amphotericin B on the permeability of lipid bilayers to divalent trace metals. | [
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10,003,796 | In this study amphotericin B released the divalent trace metals Zn2+, Co2+, Cu2+, Ni2+, Mn2+, Fe2+, Cd2+ and Pb2+ from multilamellar liposomes containing cholesterol. This observation is consistent with amphotericin B channels being permeable to these metals, and it is proposed, therefore, that the antibiotic may be useful in investigating the metabolism of these elements. | [
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10,003,797 | Structural features of fibrinogen associated with binding to chelated zinc. | [
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] |
10,003,798 | Fibrinogen absorbed to zinc chelate columns at pH 7.8 and was eluted sharply with a pK of 5.8 indicative of the involvement of a histidine residue. The binding was unaffected by high concentrations of calcium or zinc. Fibrinogen degradation products X and Y also bound to zinc chelate columns. On passage of fibrinogen fragments D and E through these columns fragment E was not bound and fragment D was eluted subsequently in a pure form with a pK of 6.1. | [
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10,003,799 | Selective arylation of cysteine-237 of rabbit muscle aldolase with 4-chloro-7-nitrobenzofurazan. | [
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] |
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