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10,000,400 | From light bulbs to CT scanners, group purchasing is filling the bill at a lower price. | [
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10,000,401 | Survey points to need for more efficient inventory management. | [
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10,000,402 | Administrators must understand and support the purchasing function. | [
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10,000,403 | Hospitals must conduct ongoing review of their policies on "disposables versus reusables'. | [
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10,000,404 | What to buy and how to get it: the cash flow factor. | [
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10,000,405 | Nonenzymatic galactosylation of human serum albumin. In vitro preparation. | [
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10,000,406 | Incubation of purified human serum albumin with D-[1-14C]galactose (5 mM) or D-[1-14C]glucose (5 mM) in vitro for 7 days under physiological conditions resulted in the time-dependent accumulation of radioactivity into trichloroacetic acid-precipitable material. Comparative studies indicated that the rate of sugar incor... | [
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10,000,407 | Heterogeneous metabolism and subcellular localization of a potentially leukemogenic membrane glycoprotein encoded by Friend erythroleukemia virus. Isolation of viral and cellular processing mutants. | [
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10,000,408 | Purification and characterization of chicken liver T-protein, a component of the glycine cleavage system. | [
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10,000,409 | T-protein, a component of the glycine cleavage system, has been purified to apparent homogeneity from chicken liver mitochondria. The molecular weight was 37,000 by sedimentation equilibrium centrifugation and 38,000 by gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis gave a molecular weight of... | [
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10,000,410 | The structure of partially oxygenated hemoglobin. A highly reactive intermediate toward a sulfhydryl titrant. | [
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10,000,411 | It is known that Cys beta 93 in hemoglobin A reacts with sulfhydryl reagents more rapidly in the oxy than in the deoxy form. In this study, the reaction of the residues toward 4,4'-dithiodipyridine was measured at various degrees of saturation with O2. Analysis of initial rates of the reaction revealed that an intermed... | [
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10,000,412 | 2-Thioflavins as active site probes of flavoproteins. | [
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10,000,413 | A non-calcitonin secretory peptide derived from preprocalcitonin. | [
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10,000,414 | Asymmetric metabolism of phosphatidylethanolamine in the human red cell membrane. | [
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10,000,415 | The incorporation of labeled fatty acids into phosphatidylethanolamine (PE) on the two sides of the human red cell membrane was studied by use of the vectorial probe trinitrobenzene sulfonate (TNBS). A small population of PE molecules on the outer surface of the membrane has a 4-fold higher turnover rate than the remai... | [
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10,000,416 | In vitro biosynthesis of the tetrasialoganglioside GQ1b. | [
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10,000,417 | A sialyltransferase activity which catalyzes the synthesis of the tetrasialoganglioside GQ1b (N-acetylneuraminyl-N-acetylneuraminylgalactosyl-N-acetylgalactosaminyl [N-acetylneuraminyl-N-acetylneuraminyl]-galactosylglucosylceramide) from added trisialoganglioside GT1b (N-=acetylneuraminylgalactosyl-N-acetylgalactosamin... | [
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10,000,418 | Effect of p-nitrophenyl-beta-D-xyloside on proteoglycan and glycosaminoglycan biosynthesis in rat serosal mast cell cultures. | [
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10,000,419 | Rat serosal mast cells cultured in the presence of heat-inactivated fetal calf serum incorporated (35S) sulfate into heparin proteoglycan of approximately Mr = 750,000 after a 3-h pulse and a 2-h chase. beta-D-Xyloside (0.1 mM) treatments of cultures of rat mast cells resulted in an insignificant increase in total (35S... | [
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10,000,420 | The effect of troponin I phosphorylation on the Ca2+-binding properties of the Ca2+-regulatory site of bovine cardiac troponin. | [
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10,000,421 | pH gradient-dependent phosphate transport catalyzed by the purified mitochondrial phosphate transport protein. | [
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10,000,422 | The mitochondrial phosphate transport protein (Wohlrab, H. (1980) J. Biol. Chem. 255, 8170-8173), which co-purifies with the adenine nucleotide translocase, has been isolated with a modified procedure resulting in an increased yield and in a preparation that is stable to storage in liquid nitrogen. The phosphate transp... | [
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10,000,423 | Irreversible coupling of immunoglobulin fragments to preformed vesicles. An improved method for liposome targeting. | [
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10,000,424 | Rabbit Fab' antibody fragments were covalently couple to preformed large unilamellar vesicles using a new sulfhydryl-reactive phospholipid derivative N-[4-(p-maleimidophenyl)butyryl]phosphatidylethanolamine (MPB-PE). A highly efficient reaction between the sulfhydryl group on each Fab' fragment and the maleimide moiety... | [
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10,000,425 | Reduced sulfhydryl groups are required for activation of uterine progesterone receptor. Possible involvement of an inhibitor of activation. | [
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10,000,426 | The dependence of uterine progesterone receptor activation on sulfhydryl groups was studied using binding to DNA-cellulose as a measure of activated receptor. Dithiothreitol, thioglycerol, or other sulfhydryl reducing reagents stimulated receptor activation 2- to 3-fold. This effect was produced under mild conditions, ... | [
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10,000,427 | Incorporation of defined cholesteryl esters into lipoproteins using cholesteryl ester-rich microemulsions. | [
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10,000,428 | Cholesteryl ester transfer to low density lipoproteins (LDL) was achieved either by incubation of a cholesteryl ester-rich lipid microemulsion with whole plasma or by incubation of the cholesteryl ester-rich microemulsion with lipoprotein-free plasma and LDL. Sequential ultracentrifugal isolation gave LDL that containe... | [
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10,000,429 | Incomplete correspondence between repressive and substrate action by amino acids on transport systems A and N in monolayered rat hepatocytes. | [
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10,000,430 | Stimulation of System N transport of glutamine by amino acid starvation of the rat hepatocyte can be repressed by one of its substrates, histidine, but not by two others, glutamine or asparagine. Furthermore, 2-(methylamino)isobutyric acid is also repressive, although it is not perceptibly a substrate or inhibitor of t... | [
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10,000,431 | Characteristics of an endogenous glucocorticoid receptor stabilizing factor. | [
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10,000,432 | Interaction of cytochalasin B with actin filaments nucleated or fragmented by villin. | [
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10,000,433 | Villin, a 95,000-dalton protein, is a major component of microvillus cores isolated from intestinal brush borders. In this study, we compared the Ca2+-dependent action of this protein on actin filaments with that of cytochalasin B, a fungal metabolite that binds to the "barbed" end of actin filaments and nuclei. We fou... | [
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10,000,434 | Heparin with two binding sites for antithrombin or platelet factor 4. | [
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10,000,435 | A new, highly discriminating affinity chromatographic technique has been developed which employs antithrombin and concanavalin A-Sepharose to fractionate heparin species of all molecular sizes. This methodology is able to subdivide the active mucopolysaccharide pools of molecular weight 6,000 to 8,000 (LMW) or 18,000 t... | [
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10,000,436 | Regulatory properties and behavior of activity of carbamoyl phosphate synthetase II (glutamine-hydrolyzing) in normal and proliferating tissues. | [
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10,000,437 | In rat livers and hepatomas, carbamoyl phosphate synthetase (glutamine-hydrolyzing) (EC 6.3.5.5) (synthetase II), the rate-limiting enzyme of de novo pyrimidine nucleotide biosynthesis, was separated from carbamoyl phosphate synthetase (ammonia) (EC 6.3.4.16) (synthetase I) ammonium sulfate and hydroxylapatite fraction... | [
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0.0... |
10,000,438 | Structures of branched blood group A-active glycosphingolipids in human erythrocytes and polymorphism of A- and H-glycolipids in A1 and A2 subgroups. | [
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10,000,439 | The structures of two branched variants of blood group A-active glycolipid, Ac and Ad (Hakomori, S., Stellner, K., and Watanabe, K. (1972) Biochem. Biophys. Res. Commun. 49, 1061-1068), isolated from human type A-erythrocytes, were elucidated. The intact glycolipids and fragments produced by treatment with endo-beta-ga... | [
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10,000,440 | Solubilization of nuclear structures by the polyanion heparin. | [
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10,000,441 | Rat liver nuclei were treated with different concentrations of the polyanion heparin, at low ionic strength and in the absence of divalent cations. Heparin at the optimal concentration (Heparin:DNA greater than or equal to 1) completely solubilized the intranuclear structures, allowing the preparation of pure nuclear m... | [
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10,000,442 | Lipid effects on the binding properties of a reconstituted insulin receptor. | [
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10,000,443 | The turkey erythrocyte membrane insulin receptor was solubilized and reconstituted into vesicles composed of either soy or dimyristoyl phosphatidylcholine. Reconstitution with soy phosphatidylcholine provided a lipid environment containing 43% unsaturated fatty acids, as compared with 82% saturated fatty acids in the d... | [
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10,000,444 | Distribution and metabolism of 3H-labeled leukotriene C3 in the mouse. | [
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10,000,445 | The distribution of [5,6,8,9,11,12-3H6]leukotriene C3 in mice was determined by whole body autoradiography of sagittal sections. Tritium was rapidly eliminated from the circulation by uptake in liver and excretion in bile as well as by renal uptake and excretion in urine. Chromatographic analyses of tritium in tissues ... | [
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10,000,446 | Changes in tropomyosin during development of chick embryonic skeletal muscles in vivo and during differentiation of chick muscle cells in vitro. | [
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10,000,447 | We have studied the changes in tropomyosin subunits and their pattern of phosphorylation during embryonic development and also during in vitro differentiation of cultured muscle cells. We have found that in 10-day-old embryos tropomyosin consists of 75% beta subunit and 25% alpha subunit. However, only 60% of the beta ... | [
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10,000,448 | Glycosphingolipids of rat tissues. Different composition of epithelial and nonepithelial cells of small intestine. | [
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10,000,449 | The epithelial cells of rat small intestine (jejunum-ileum) were separated from their supporting stroma (residue). Total nonacid and acid glycosphingolipids were prepared from the two compartments. The acetylated nonacid glycolipids were separated into 10-12 fractions by column chromatography. These were analyzed by ch... | [
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10,000,450 | Active subunits in hybrid-modified malate dehydrogenase. | [
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10,000,451 | Inactivation of porcine heart mitochondrial malate dehydrogenase (L-malate: NAD+ oxidoreductase, EC 1.1.1.37) by selective modification of an active center histidine residue with the reagent iodoacetamide has been further investigated to examine the existence of and the enzymatic activity of a hybrid (half)-modified di... | [
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10,000,452 | Favored sites for thyroid hormone formation on the peptide chains of human thyroglobulin. | [
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10,000,453 | Intracellular glycosylation of vitellogenin in the liver of estrogen-stimulated Xenopus laevis. | [
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10,000,454 | Pulse-chase experiments measuring the rates of incorporation of radiolabeled glucosamine and galactose into intracellular vitellogenin show that glycosylation of this multicomponent protein occurs in a Golgi-enriched fraction isolated from homogenized liver slices. No apparent role of the rough endoplasmic reticulum in... | [
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10,000,455 | The epidemiology of high density lipoprotein cholesterol levels in post-menopausal women. | [
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10,000,456 | High density lipoprotein cholesterol (HDLc) appears to be the most important risk factor of heart attack where increased HDLc levels are associated with reduced heart attack risk. Little is known regarding the determinants of HDLc in older women. The current research investigated possible HDLc correlates in 75 post-men... | [
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10,000,457 | Sex differences in life satisfaction among the elderly. | [
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10,000,458 | Sex differences in life satisfaction are a recurrent research issue in the field of social gerontology. However, the evidence concerning the effect of sex on morale is still inconclusive. This may be due to (a) the neglect of other relevant variables, (b) the failure to distinguish between main effects and interaction ... | [
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10,000,459 | Adaptation to marital separation in later and earlier life. | [
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10,000,460 | In recent years, the divorce rate has increased markedly for people of all ages. Whereas the consequences of the divorce experience for those in young adulthood have received considerable attention, relatively little is known about their impact on older adults. This paper evaluates the psychosocial functioning of recen... | [
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10,000,461 | Time budgets of older people:" a window on four lifestyles. | [
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10,000,462 | The purposes of this research were first to describe how a group of urban elderly spend their days and, second, to examine the responsiveness of the behavioral day to rather marked environmental and personal differences. Detailed time budgets for one full day (including the content, duration, social and environmental c... | [
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10,000,463 | Myocardial kinetics and organ distribution of thallium-201 in two different age groups of rats. | [
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10,000,464 | Effects of aging were investigated on kinetics and distribution of 201 thallium (201TI) in isolated myocardial tissue and other organs of rats after injection in vivo of this radionuclide. Older rats showed a trend toward lesser organ uptake, but this was statistically significant only in kidneys, fat, and fur. Experim... | [
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10,000,465 | Age-related changes in monosynaptic reflex excitability. | [
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10,000,466 | The excitability of the H-reflex was tested in a younger group (19 to 31 years) and an older group (60 to 72 years) of subjects. The threshold for the H-reflex was higher and the reflex latency was 7 msec longer in the older group of subjects. Also, the H-reflex amplitude was significantly smaller, longer in duration a... | [
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10,000,467 | Ploidy of human embryonic fibroblasts during in vitro aging. | [
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10,000,468 | The DNA content of single nuclei in confluent cultures of aging human embryonic fibroblasts, TIG-3, was measured by means of flow cytofluorometry. In the early and late stages of their in vitro lifespan, they had considerable numbers of 4C, 8C and 16C nuclei. In the other period more than 95% of their nuclei were 2C nu... | [
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10,000,469 | Inverse correlation between species life span and specific cytochrome P-448 content of cultured fibroblasts. | [
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10,000,470 | The quantity and type of microsomal cytochrome P-450 was determined in early passage cultured fibroblasts from six mammalian species of widely differing life spans. The proportion of cytochrome P-450 and P448 varied considerably among species, with the shorter-lived species having a preponderance of cytochrome P-448. T... | [
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10,000,471 | Emotionality and aging: a theoretical and empirical analysis. | [
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10,000,472 | Much has been written about mood, emotionality, and affect in humans, but rarely has this topic been addressed from an adult developmental perspective. Indeed, the major theories on the determinants of human emotions have little to say about emotionality through middle and old age. Theories and data from several discip... | [
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0.027358081191778183,
0.03255420... |
10,000,473 | A comparison of the emitted late positive potential in older and young adults. | [
0.02521754615008831,
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10,000,474 | An emitted potential paradigm was used to investigate P3 scalp potentials in a group of older individuals (M = 66.8 years) and a group of younger persons (M = 22.0 years). Since no physical stimulus is necessary to elicit an emitted potential, an age comparison of P3 can be made which does not depend on normal sensory ... | [
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10,000,475 | Isolating the age deficit in speeded performance. | [
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10,000,476 | The 'locus' of the age-associated behavioral slowing was investigated with Sternberg's additive-factor method of identifying information processing stages. The factor of adult age was found to interact with manipulations assumed to influence stages concerned with stimulus encoding, internal comparison, and response pre... | [
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10,000,477 | Sucrose taste thresholds: age-related differences. | [
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10,000,478 | Sucrose taste detection thresholds were measured in 71 adults aged 20 to 88 years. A forced-choice, tracking procedure was used to eliminate subject response bias and rinses were included between all stimuli. A significant age-related decrease in sensitivity was observed. However, the loss of sensitivity was not great ... | [
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10,000,479 | Adult age similarities in free recall output order and strategies. | [
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10,000,480 | Adult age differences on a variety of free recall measures were examined. Although primary memory capacity was found to be the same in young and old adults, there was a smaller recency effect in the older group. Recall of primacy items was also less for that group. However, the pattern of serial position effects was th... | [
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10,000,481 | Aging and the picture superiority effect in recall. | [
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10,000,482 | One recurrent theme in the literature on aging and memory is that the decline of memory for nonverbal information is steeper than for verbal information. This research compares verbal and visual encoding using the picture superiority effect, the finding that pictures are remembered better than words. In the first exper... | [
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10,000,483 | Coding of spatial information by young and elderly adults. | [
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10,000,484 | Two experiments are reported in which the presumed automatic nature of spatial coding is examined. In Experiment 1, 48 young and 48 elderly female adults, half of each group aware and half unaware of the task requirements, studied 16 cards with four nouns arranged vertically on each card. They were then given these 64 ... | [
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10,000,485 | Estimates of age changes on the Benton Visual Retention Test. | [
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10,000,486 | Performances on the Benton Visual Retention Test were collected for 894 men 17 to 96 years of age between 1960 and 1976 and were analyzed using slopes to estimate time-of-measurement effects. For each of the nine birth cohorts and eight age groups, performance (errors) was plotted on calendar time. Comparisons of the w... | [
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10,000,487 | A research and service oriented multilevel assessment instrument. | [
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10,000,488 | An assessment instrument capable of measuring the wellbeing of the aged in a number of significant domains is described. This Philadelphia Geriatric Center Multilevel Assessment Instrument (MAI) systematically assesses behavioral competence in the domains of health, activities of daily living, cognition, time use, and ... | [
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10,000,489 | Biochemical evidence for the coexistence of monomorphic and polymorphic N-acetyltransferase activities on a common protein in rabbit liver. | [
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10,000,490 | New Zealand White rabbits were classified as rapid or slow acetylators by measuring the N-acetylation half-life of i.v. administered sulfadiazine, by pedigree analysis and by determination of liver N-acetyltransferase (NAT) activity in vitro. Slow acetylator rabbits were found to have significantly lower levels of p-am... | [
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10,000,491 | Inhibition of [3H]norepinephrine uptake in organ cultured rat superior cervical ganglia by xylamine. | [
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10,000,492 | The superior cervical ganglia (SCG) of the rat, when maintained in organ culture, show an increase in [3H]norepinephrine (NE) uptake in the first 2 days and then a leveling off of this activity through 6 days of culture. The [3H]NE uptake in cultured SCG is Na+ - and temperature-dependent and is inhibited by cocaine, d... | [
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10,000,493 | Antipyrine kinetics in the elderly: prediction of age-related changes in benzodiazepine oxidizing capacity. | [
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0.0... |
10,000,494 | Comparison of the behavioral effects of phencyclidine, ketamine, d-amphetamine and morphine in the squirrel monkey. | [
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10,000,495 | Augmentation by theophylline of [3H]purine release from vascular adrenergic nerves: evidence for presynaptic autoinhibition. | [
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10,000,496 | The effect of theophylline (a P1-purinoceptor blocker) on the tritium release evoked by high KCl (50 mM) and l-epinephrine (3 microM) was evaluated using [3H]adenosine-prelabeled pulmonary artery of the rabbit. It has previously been shown that KCl elicits purine release from adrenergic nerve terminals whereas epinephr... | [
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0.07720... |
10,000,497 | Effects of dantrolene sodium on the electrophysiological properties of canine cardiac Purkinje fibers. | [
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0.0... |
10,000,498 | Dantrolene sodium at concentrations between 8.8 and 35.1 microM produced rather selective changes in the electrophysiological properties of isolated dog Purkinje fibers. The action potential duration at 90% repolarization and effective refractory period of normally polarized fibers were increased in a dose- and frequen... | [
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0.021548733115196228,
0.05065480... |
10,000,499 | Presynaptic effects of tetrahydropapaveroline on striatal dopaminergic neurons. | [
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0.016640624031424522,
0.000204076204681769,
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0.058... |
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