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10,004,400 | A popular colorimetric analysis for serum acetaminophen, based on ring-nitration of the drug, is demonstrated to yield both "false positives" and erroneously high results for serum from uremic patients. The interference appears to be anionic at physiological pH, and correlates significantly with serum creatine concentr... | [
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10,004,401 | Macro creatine kinase: determination and differentiation of two types by their activation energies. | [
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10,004,402 | Determination of the MB isoenzyme of creatine kinase in patients with acute myocardial infarction may be disturbed by the presence of macro creatine kinase. The relative molecular mass of this form of creatine kinase in human serum is at least threefold that of the ordinary enzyme, and it is more thermostable. Here we ... | [
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10,004,403 | Glucose determinations in plasma and serum: potential error related to increased hematocrit. | [
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10,004,404 | This study reports the effect of increased hematocrit (packed cell volume) on the rate of decrease in plasma glucose during 6 h in blood specimens from seven newborns (cord blood) and heparin-treated blood specimens from eight adults. Mean hematocrits ranged frm 0.43 to 0.75 for the adults and from 0.51 to 0.81 for the... | [
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10,004,405 | Enzymic determination of citrate in serum and urine, with use of the Worthington "ultrafree" device. | [
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10,004,406 | We describe an enzymic method for conveniently measuring citrate in serum or urine. Interfering enzyme proteins are removed by a disposable ultrafilter ("Ultrafree"; Worthington Diagnostics), obviating the need for hazardous protein precipitants. A 50 mmol/L Tris buffer adequately controls pH, no lactate dehydrogenase ... | [
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10,004,407 | Influence or age and sex on the kinetics of intravenously administered L-phenylalanine. | [
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10,004,408 | We studied the kinetics of intravenously administered L-phenylalanine with respect to the effect of age and sex, using a two-compartment model. We found that the volume of the peripheral compartment and total body clearance decrease with age. The sex-related influence was less obvious when distribution volumes and tota... | [
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10,004,409 | Improved one-dimensional thin-layer chromatography of phospholipids in amniotic fluid. | [
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10,004,410 | With this extraction procedure and chromatographic method, six amniotic fluid phospholipids [phosphatidylcholine (lecithin), sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol] are completely resolved in less than 70 min from the time the specimen is received. Th... | [
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10,004,411 | Determination of phenobarbital and phenytoin by gas-liquid chromatography with on-column methylation. | [
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10,004,412 | Parathyrin (parathyroid hormone): radioimmunoassays for intact and carboxyl-terminal moieties. | [
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10,004,413 | Circulating IgG-LD complex, dissociable by addition of NAD+. | [
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10,004,414 | Macromolecular LD (lactate dehydrogenase, EC 1.1.1.27) was present in the serum of a patient suffering from idiopathic fibrosis of the lung and presenting signs of autoimmune disease. By using gel filtration and affinity chromatography techniques, the vast majority of the patient's serum LD activity was shown to consis... | [
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10,004,415 | Increase in serum alkaline phosphatase owing to furosemide. | [
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10,004,416 | Rapid detection and estimation of hemoglobins by agarose gel electrophoresis. | [
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10,004,417 | Misuse of glucose oxidase test strips. | [
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10,004,418 | Solid-phase radioimmunoassay of conjugated bile acid. | [
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10,004,419 | Superoxide dismutase, glutathione peroxidase, and catalase activities in the erythrocytes of patients with intermittent acute porphyria. | [
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10,004,420 | Evaluation of a kit for assessing cystinuria and homocystinuria. | [
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10,004,421 | Improved gas-liquid chromatographic method for measurement of mexiletine in plasma. | [
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10,004,422 | Novel thin-layer chromatographic visualization reagent for phencyclidine. | [
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10,004,423 | Determination of thioethers in urine. | [
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10,004,424 | Quantitation of urinary normetanephrine and metanephrine by reversed-phase extraction and mass-fragmentographic analysis. | [
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10,004,425 | Hydrolyzed urine with added ring-trideuterated normetanephrine and metanephrine is applied to wet C18-reversed-phase minicolumns. The "metanephrines" are eluted, dried, derivatized with pentafluoropropionic anhydride, and analyzed with the gas chromatograph-mass spectrometer. Ions for the nondeuterated and trideuterate... | [
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10,004,426 | Upward drift, rather than compression of sample-line air segments, as an important source of error in routine operation of the Technicon SMAC system. | [
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10,004,427 | Interference in assay of human thyrotropin by endogenous antibodies to bovine thyrotropin. | [
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10,004,428 | Improved method for determination of "true" creatinine. | [
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10,004,429 | Stability of the composite solution [Mercury (II)-TPTZ-iron(II)] used for the automated determination of serum chloride. | [
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10,004,430 | Liquid-chromatographic determination of the total thiamin content of blood. | [
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10,004,431 | A liquid-chromatographic method for determining the total thiamin content of blood is presented. Blood is deproteinized and incubated with Aspergillus oryzae carboxyl proteinase (EC 3.4.23.6; Takadiastase) to convert thiamin phosphate esters to free thiamin. An aliquot of the sample is applied to the column (Shodex OH-... | [
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10,004,432 | Liquid-chromatographic determination of ethylene glycol in plasma. | [
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10,004,433 | In this novel procedure for determining ethylene glycol in plasma by liquid chromatography, benzoyl esters of ethylene glycol and of benzyl alcohol (used as the internal standard) are prepared directly in plasma. The benzoyl esters, highly ultraviolet-absorbing chromogens, are ideal compounds for analysis by reversed-p... | [
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10,004,434 | Electrophoretic determination of isoamylases in serum with commercially available reagents. | [
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10,004,435 | We describe and evaluate a method for determining isoamylases in serum. With this method, which involves conventional electrophoretic apparatus and commercially available reagents, seven isoenzymes can be resolved. The electrophoresis is performed in agarose gel, and isoenzymes are detected with a water-insoluble cross... | [
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10,004,436 | Stability of creatine kinase-3 in lyophilized materials. | [
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10,004,437 | We examined the stability of creatine kinase (EC 2.7.3.2) isoenzyme-3 (CK-3) in lyophilized bovine albumin matrices in the presence and absence of various sulfhydryl compounds and ADP. We initially purified CK-3 from human myocardium and skeletal muscle by the batch-chromatographic technique and by gradient elution col... | [
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... |
10,004,438 | Characterization of uremic "middle molecular" fractions by gas chromatography, mass spectrometry, isotachophoresis, and liquid chromatography. | [
0.005612784996628761,
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0.... |
10,004,439 | Uremic ultrafiltrates (and normal serum, for comparison) were fractionated by means of gel filtration. The collected fractions were further investigated by combined analytical techniques: "high-performance" liquid chromatography, gas chromatography, mass spectrometry, and isotachophoresis. Ultrafiltrate fractions in th... | [
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... |
10,004,440 | Influence of erythrocyte iodothyronine-binding proteins on radioimmunoassay of thyroxin in dried blood spots. | [
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0.0516... |
10,004,441 | Three erythrocyte proteins, one identified as hemoglobin, bind thyroid hormones. Using a dextran/charcoal radioimmunoassay for thyroxin in dried blood spots, we demonstrate that such binding differs with the buffer used. Barbital, phosphate, and borate buffers significantly enhance the binding more than glycine and tri... | [
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0.051698... |
10,004,442 | Development and validation of a new radioimmunoassay for parathyrin (PTH). | [
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0.09... |
10,004,443 | A radioimmunoassay for human parathyrin has been developed and characterized with whole-molecule (residues 1-84) human parathyrin and with the 1-34, 44-68, and 53-84 amino acid residue fragments of it. The antiserum used reacted with the whole molecule and with the 44-68 and 53-84 fragments, but not with the 1-34 fragm... | [
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0.043... |
10,004,444 | Gas-chromatographic determination of ethylene glycol in serum. | [
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-0.... |
10,004,445 | We describe a new gas-chromatographic procedure for ethylene glycol in serum, determined as the cyclic phenylboronate ester. The method is rapid, requires only 100 microL of serum, and either an OV-1 or an OV-17 column is used. The linear dynamic range extends to at least 5000 mg/L, with a lower limit of detection of 1... | [
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-... |
10,004,446 | More sensitive flameless atomic absorption analysis of vanadium in tissue and serum. | [
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0.020258342... |
10,004,447 | We describe a sensitive method for the determination of vanadium in biological substances by atomic absorption spectroscopy. By using background correction and pyrolytically coated graphite tubes with flameless apparatus, this vanadium assay has a sensitivity of 65 pg and a detection limit of 30 pg, approximately sixfo... | [
0.08386518061161041,
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0.02524... |
10,004,448 | Radioimmunoassay of serum parathyrin: comparison of five commercial kits. | [
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0.0... |
10,004,449 | We compared serum parathyrin radioimmunoassay data obtained with five commercially available kits for normal subjects, patients on dialysis, and patients with hyperparathyroidism. The Cambridge Nuclear kit gave low results, did not distinguish the dialysis-group sera from the normal sera, and had the greatest CV (56%) ... | [
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10,004,450 | Hemoglobin A1C by isoelectric focusing. | [
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0.... |
10,004,451 | We separated the hemoglobins in an unwashed erythrocyte hemolysate according to their isoelectric points on a thin-layer horizontal polyacrylamide gel containing ampholyte (40 g/L) over a pH gradient of 6-8. We scanned the fixed, unstained gels by microdensitometry and calculated the percentage of hemoglobin A1C. The o... | [
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0.04... |
10,004,452 | Improved method for determining erythrocyte creatine by the diacetyl-alpha-naphthol reaction: elimination of endogenous glutathione interference. | [
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0.0... |
10,004,453 | We describe a simple, accurate, and reproducible method for determining erythrocyte creatine. The method is free from glutathione inhibition and is adaptable to use with standard spectrophotometers as well as centrifugal analyzers. A clear filtrate, essentially free of protein, hemoglobin, and glutathione, is prepared ... | [
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0.... |
10,004,454 | New spectrophotometric method for the determination of hemoglobin A1 compared with a microcolumn technique. | [
0.05116312950849533,
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-0.06452545523643494,
0.01921... |
10,004,455 | We compared a spectrophotometric kit method (Glycospec) for determination of glycosylated hemoglobin (HbA1) with a microcolumn kit method (Bio-Rad). The Glycospec method is based on the change in absorbance when phytic acid binds to hemoglobin A. With glycosylated hemoglobin there is no such change because the binding ... | [
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0.014280910603702068,
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-0.01521... |
10,004,456 | The multivariate reference range: an alternative interpretation of multi-test profiles. | [
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10,004,457 | To test the utility of multivariate interpretation of laboratory data, we developed a multivariate reference range based of the Mahalonobis distance (D2) measure. Results for 20 commonly measured clinical chemistry analytes were collated for two sophomore medical-school classes (118 and 143 individuals in 1979 and 1980... | [
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0.0... |
10,004,458 | Effectiveness of analysis of free thyroxin concentration in serum for diagnosis of covert thyroid disease. | [
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0.0... |
10,004,459 | The indirect indices of free-thyroxin concentration (fT4) are of limited effectiveness in diagnosing covert (mild) thyroid disease. We compared measurements of fT4 and of normalized T4 (NT4, the effective thyroxin ratio) in a group of patients being treated for thyroid disease, most of whom were clinically euthyroid. A... | [
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0.0225... |
10,004,460 | ILIS--An integrated laboratory information system. I. Biochemistry and hematology. | [
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0.034... |
10,004,461 | IBM System 34 (central processing unit, 128 kilobytes; fixed disks, 128.4 megabytes) with seven cathode-ray tubes has been used by our clinical laboratories for the last 30 months. All data-entry programs are in a conversational mode, for on-line corrections of possible errors in patient identification and results. Dai... | [
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0.06458914279937744,
-0.0078... |
10,004,462 | Creatine kinase and creatine kinase B-subunit activity in serum in cases of suspected myocardial infarction. | [
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0.0... |
10,004,463 | We evaluated a diagnostic strategy by studying 481 patients suspected of having had an acute myocardial infarction; the prevalence of infarction by independent criteria was 0.43. This strategy is based on the sequential application of: (a) clinical criteria; (b) total creatine kinase determinations in two serum samples... | [
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0.039... |
10,004,464 | A candidate reference method for uric acid in serum. I. Optimization and evaluation. | [
0.04363539069890976,
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0.0017539015971124172,
0.054... |
10,004,465 | We describe our optimization and evaluation of a candidate Reference Method for uric acid in serum. Reaction parameters were optimized for a manual, enzymic method for uric acid in which highly purified microbial uricase is used to quantitate uric acid by a differential ultraviolet procedure. We evaluated the method in... | [
0.0003588241816032678,
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0.01687277853488922,
0.01929651014506817,
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-0.00475511746481061,
0.062... |
10,004,466 | A candidate reference method for uric acid in serum. II. Interlaboratory testing. | [
0.018083985894918442,
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-0.0008635980193503201... |
10,004,467 | We describe the interlaboratory testing of a candidate Reference Method (Part I) for uric acid in serum. The method is based on the ultrasound spectrophotometric quantitation of uric acid before and after incubation with uricase. A comprehensive investigation involving 12 laboratories was organized to document the tran... | [
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0.0... |
10,004,468 | On individual reference intervals based on a longitudinal study of plasma proteins and lipids in healthy subjects, and their possible clinical application. | [
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0.03... |
10,004,469 | In a longitudinal study, we determined interindividual and intra-individual variation in 20 plasma proteins and lipids and in other blood constituents by analysis of variance. Blood from 20 healthy subjects was sampled monthly for six months, a rigorous blood-sampling technique being applied. The mean proportion of int... | [
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0.07066062092781067,
0.06486055254936218,
0.071... |
10,004,470 | Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes. | [
0.08373663574457169,
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0.0633746013045311,
-0.... |
10,004,471 | We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC 1.11.1.9), in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies ... | [
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0.02375829592347145,
-0.001759... |
10,004,472 | Creatine kinase isoenzymes in serum from cord blood and the blood of healthy full-term infants during the first three postnatal days. | [
-0.036199744790792465,
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-0.0995512530207634,
0.048... |
10,004,473 | Isoenzymes of creatine kinase (ATP:creatine phosphotransferase; EC 2.7.3.2; CK) were measured by electrophoresis in serum from cord blood and skin-puncture blood taken from 45 healthy full-term infants during the first three postnatal days. Mean total CK activities (in U/L at 30 degrees C) were 185 in cord samples, 536... | [
0.0003322928969282657,
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0.02337413839995861,
0.07049217075109482,
-0.06692255288362503,
-0.03818957507610321,
0.01... |
10,004,474 | Laboratory differentiation of hypercalcemic patients. | [
0.08045943081378937,
0.0051686884835362434,
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0.030581476166844368,
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0.00716715632006526,
0.027264315634965897,
-0.062365513294935226,
-0.08522633463144302,
0.02... |
10,004,475 | We studied the biochemical discrimination of primary hyperparathyroidism from other causes of hypercalcemia. This potentially serious disorder is readily remedied, and because recent work has suggested that even very mild cases in elderly people derive substantial benefit from surgery, the correct diagnosis is crucial.... | [
0.013967216946184635,
0.0659804716706276,
0.03298267722129822,
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0.008600041270256042,
0.023554541170597076,
-0.01391806360334158,
0.06940282881259918,
0.03971254453... |
10,004,476 | Improved procedure for lecithin/sphingomyelin ratio in amniotic fluid reduces false predictions of lung immaturity. | [
0.045378781855106354,
0.0026730571407824755,
-0.05005447566509247,
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0.08151664584875107,
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-0.006552616134285927,
-0.01344228070229292,
-0.050323717296123505,
-0.0023301406763494015,
... |
10,004,477 | Many laboratories have found that their procedure for determining lecithin/sphingomyelin (L/S)ratios gives an unacceptably high proportion of false predictions of fetal lung immaturity. We investigated each step in the procedure, in an attempt to improve the clinical performance of the test and to make the method more ... | [
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0.041165705770254135,
0.05431012064218521,
-0.016406670212745667,
-0.0008460758253931999,
... |
10,004,478 | Methylene unit calibration for analysis of organic acids by gas--liquid chromatography/mass spectrometry. | [
0.04668315500020981,
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-0.08016379177570343,
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0.11006594449281693,
0.03302859887480736,
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0.055070359259843826,
0.045361410826444626,
-0.04060276597738266,
0.022339479997754097,
-0.10999208688735962,
-0.02952258102595806,
-0.03... |
10,004,479 | We describe a method for automatic computation of "methylene unit" retention-time indices in real time on a computer-controlled gas chromatograph/mass spectrometer system. The calculation is based on the exponential relationship between the methylene unit values of hydrocarbon reference standards and the oven temperatu... | [
0.011704379692673683,
0.01442632544785738,
-0.11951538920402527,
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0.013579186983406544,
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0.03226981312036514,
-0.09115470945835114,
-0.008369220420718193,
-0.0913054347038269,
0.009817821905016899,
-0.020... |
10,004,480 | Radioimmunoassay for hepatitis B core antigen. | [
0.01624634861946106,
0.010109898634254932,
-0.12829144299030304,
-0.09478684514760971,
-0.05647767335176468,
0.0585453063249588,
0.06681019067764282,
0.10815377533435822,
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0.02779233269393444,
-0.02750081568956375,
-0.05390668287873268,
0.050170157104730606,
0.03523669... |
10,004,481 | Serum hepatitis B core antigen (HBcAg) is an important marker of hepatitis B virus replication. We describe an easy, sensitive radioimmunoassay for determination of HBcAg in detergent-treated serum pellets containing Dane particles. Components of a commercial kits for anti-core determination are used, and HBcAG is meas... | [
0.01416175439953804,
0.00005341026917449199,
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-0.027424480766057968,
-0.07969634979963303,
0.06834383308887482,
-0.002... |
10,004,482 | The cerebrospinal fluid immunoglobulin G index as a diagnostic aid in multiple sclerosis: a Bayesian approach. | [
0.01579958386719227,
-0.06502332538366318,
0.06294526904821396,
-0.03379625082015991,
0.016620120033621788,
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0.12194875627756119,
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0.028124257922172546,
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0.011890632100403309,
0.050881534814834595,
0.0591... |
10,004,483 | Having determined immunoglobulin G (IgG) and albumin concentrations in 1100 cerebrospinal fluid and serum samples, we calculated the IgG index. Likelihood ratios for multiple sclerosis were calculated by using a training set consisting of 100 patients with definite multiple sclerosis and one consisting of 97 patients s... | [
0.01655324548482895,
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0.014733139425516129,
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0.03309806436300278,
-0.08802244067192078,
-0.031091032549738884,
0.05933384597301483,
0.0306797... |
10,004,484 | Six methods for urinary protein compared. | [
0.007581244222819805,
-0.08027806878089905,
0.004863250534981489,
-0.03065752610564232,
-0.06243716552853584,
0.003119621891528368,
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0.12349849939346313,
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0.007618648000061512,
0.059563472867012024,
-0.08855389058589935,
0.099... |
10,004,485 | Six assays for protein in urine were compared for linearity, ease of standardization, precision, comparability of assay values, technical ease of assay, and current cost. The assays investigated were three dye-binding techniques, a recent turbidimetric technique, the trichloroacetic acid--biuret reaction, and a tannic ... | [
-0.028061244636774063,
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0.02869010716676712,
-0.01759626530110836,
0.0796099... |
10,004,486 | Gastrin radioimmunoassay. | [
0.012366068549454212,
0.07055722922086716,
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0.07054576277732849,
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0.009308405220508575,
0.00680942228063941,
-0.02029883675277233,
0.09497... |
10,004,487 | A variant alkaline phosphatase in renal cell carcinoma. | [
-0.03319026902318001,
0.03840363770723343,
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0.004028323572129011,
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0.011502875946462154,
-0.... |
10,004,488 | We report a case of renal cell carcinoma in which up to 32% of the abnormally increased alkaline phosphatase activity in serum was contributed by a variant alkaline phosphatase originating in the primary tumor and its secondary deposits. The variant enzyme was probably an altered form of normal renal alkaline phosphata... | [
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0.018057806417346,
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-0.017673293128609657,
0.011504719965159893,
0.01071... |
10,004,489 | A bichromatic technique for serum iron on the CentrifiChem 400. | [
0.06330873817205429,
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-0.009067750535905361,
0.03161432221531868,
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0.013941604644060135,
-0.013... |
10,004,490 | Comments on interference from bilirubin in a system for HDL-cholesterol determination. | [
0.006810634862631559,
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0.02430468611419201,
0.012709599919617176,
-0.0323... |
10,004,491 | Combined enzymic-Jaffé method for creatinine in serum. | [
-0.045232273638248444,
-0.07976378500461578,
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0.04654755815863609,
0.02252889983355999,
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0.10319554060697556,
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-0.08716720342636108,
-0.01539171114563942,
-0.038701388984918594,
0.013382094912230968,
0.060... |
10,004,492 | Improved estimation of formate in body fluids and tissues. | [
0.02731219120323658,
-0.0778915286064148,
0.035856943577528,
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0.008993752300739288,
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0.009291079826653004,
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-0.03944648057222366,
-0.046894170343875885,
0.122764... |
10,004,493 | Improved measurement of low concentrations of conjugated bilirubin with the DuPont aca. | [
0.025791997089982033,
-0.06007865071296692,
-0.05926474928855896,
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-0.00021793920313939452,
-0.005760325118899345,
0.03178511932492256,
... |
10,004,494 | Glycosylated protein in whole blood spotted on filter paper. | [
-0.07869419455528259,
-0.020558083429932594,
-0.02898440696299076,
0.0027522663585841656,
0.05478416383266449,
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0.05013216659426689,
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0.04437469318509102,
0.0516914427280426,
-0.06883832067251205,
0.0802506... |
10,004,495 | Even easier measurement of viscosity of semi-micro serum samples. | [
0.029446253553032875,
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-0.00527964998036623,
0.016854891553521156,
0.05220649763941765,
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-0.06663532555103302,
-0.03170783072710037,
0.09... |
10,004,496 | Fluoride and iodoacetate interfere with glucose determination with the Yellow Springs Glucose Analyzer. | [
-0.023706994950771332,
0.011917920783162117,
-0.06305670738220215,
0.02293902263045311,
0.11712329834699631,
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0.020604554563760757,
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0.029874727129936218,
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0.008983450010418892,
-0.07234707474708557,
0.04... |
10,004,497 | Some lots of xylene contain substance(s) interfering with tocopherol assay. | [
0.028902197256684303,
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0.013496684841811657,
0.09412398934364319,
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0.02812386304140091,
0.04935967177152634,
0.010595238767564297,
0.013398976996541023,
0.058837149292230606,
0.016031567007303238,
0.03166033700108528,
0.0914... |
10,004,498 | A discrete analyzer (ABA-100) method for prostatic acid phosphatase with thymolphthalein monophosphate as substrate. | [
-0.058932073414325714,
0.04142707958817482,
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0.057057950645685196,
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-0.005745544098317623,
-0.012157080695033073,
0.... |
10,004,499 | Two-step assay of erythrocyte transketolase activity. | [
0.0066777984611690044,
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-0.06446031481027603,
0.03521107882261276,
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0.07279738783836365,
0.08261637389659882,
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-0.0006828323821537197,
-0.06244056671857834,
-0.04674508795142174,
-0.0705239549279213,
0.... |
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