id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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64,387 | SIMPLI ACV KETO GUMMIES REVIEWS *PROS & CONS* SHOCKING REPORTED ABOUT SIDE EFFECTS? | 1 | dx.doi.org/10.17504/protocols.io.n2bvj6bmwlk5/v1 | https://www.protocols.io/view/simpli-acv-keto-gummies-reviews-pros-amp-cons-shoc-ca5bsg2n | miketisen | TITLE: SIMPLI ACV KETO GUMMIES REVIEWS *PROS & CONS* SHOCKING REPORTED ABOUT SIDE EFFECTS?
AUTHORS: miketisen
[DESCRIPTION]
Simpli ACV Keto Gummies Reviews (Simpli Health ACV Gummies) Weight Loss WEBSITE Where to Buy
[STEPS]
1. People are gaining excessive body fat because they do not take care of their healt... | ["People are gaining excessive body fat because they do not take care of their health. People eat unhealthy food day and night and worry about how they are getting obese. This happens because of our negligence. If we follow an unhealthy routine daily and will not eat healthy food on time, then we can attract obes... |
60,312 | Direct nuclear tagmentation and RNA-sequencing (DNTR-seq) | 1 | null | https://www.protocols.io/view/direct-nuclear-tagmentation-and-rna-sequencing-dnt-b65yrg7w | Vasilios Zachariadis, Huaitao Cheng, Nathanael Andrews, Martin Enge | TITLE: Direct nuclear tagmentation and RNA-sequencing (DNTR-seq)
AUTHORS: Vasilios Zachariadis, Huaitao Cheng, Nathanael Andrews, Martin Enge
[DESCRIPTION]
Understanding how genetic variation alters gene expression - how genotype affects phenotype - is a central challenge in biology. To address this question in comple... | ["[Prepare lysis buffer plates for cell sorting] Prepare lysis buffer mix\n\nNOTE: Reagents are prepared on ice, working quickly. ERCC is stored in single-use aliquots at -80 °C , thawed on ice and added last. \n \n rxn13115228848006000H2O1,313,931509,12377,2862887860RNase Inhibitor0,050,1557,614,4240300ERCC (1:1... |
45,867 | Hepatorenal index protocol | 1 | dx.doi.org/10.17504/protocols.io.bq2jmycn | https://www.protocols.io/view/hepatorenal-index-protocol-bq2jmycn | fabio10stahl , Fabio Lucio Stahlschmidt | TITLE: Hepatorenal index protocol
AUTHORS: fabio10stahl , Fabio Lucio Stahlschmidt
[STEPS]
?. Each patient fasted for 4 h before the examination. First, the radiologist performed a comprehensive thoracic and abdominal ultrasound examination using the same ultrasound machine for all patients (S2000 HELX; Siemens, Erlan... | ["Each patient fasted for 4 h before the examination. First, the radiologist performed a comprehensive thoracic and abdominal ultrasound examination using the same ultrasound machine for all patients (S2000 HELX; Siemens, Erlangen, Germany). For all examinations, the conditions were as follows: frequency of 4.00 MHz, d... |
65,790 | RNA EXTRACTION FROM WHEAT STIGMAS STORED IN DNA/RNA SHIELD | 4 | dx.doi.org/10.17504/protocols.io.36wgq7kj3vk5/v1 | https://www.protocols.io/view/rna-extraction-from-wheat-stigmas-stored-in-dna-rn-ccg6stze | Marina Millán Blánquez | TITLE: RNA EXTRACTION FROM WHEAT STIGMAS STORED IN DNA/RNA SHIELD
AUTHORS: Marina Millán Blánquez
[DESCRIPTION]
Despite the importance of understanding the molecular processes plants go through under field conditions, transcriptomic studies are often difficult to perform on field samples since access to liquid nitro... | ["[Phase separation] Add two steel ball bearings into each Eppendorf tube containing the samples preserved in DNA/RNA Shield solution (~200 μL should be enough for five stigmas at Waddington stage 10 – i.e., stigmatic branches spreading outwards) (Fig. 1)", "[Phase separation] Grind stigmas in DNA/RNA Shield solution f... |
101,702 | Determination of Auxenochlorella protothecoides Cell Density With a Hemocytometer | 0 | dx.doi.org/10.17504/protocols.io.rm7vzjk12lx1/v1 | https://www.protocols.io/view/determination-of-auxenochlorella-protothecoides-ce-dfje3kje | Dimitrios Camacho, Sabeeha Merchant | TITLE: Determination of Auxenochlorella protothecoides Cell Density With a Hemocytometer
AUTHORS: Dimitrios Camacho, Sabeeha Merchant
[DESCRIPTION]
This protocol describes a reliable method for counting the cell densities of Auxenochlorella protothecoides cultures using a Neubauer hemocytometer. This method is accurat... | ["[Procedure] Move the culture flask to a clean sterile hood.", "[Procedure] Swirl the culture several times to ensure thorough mixing.", "[Procedure] Hold the stage clip open, set the hemocytometer on the microscope stage, and release the stage clip gently to secure the hemocytometer.", "[Procedure] Remove the dust co... |
null | null | null | dx.doi.org/10.17504/protocols.io.ekubcww | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This collection contains protocols for the computational analysis performed in the paper "The Human Skin dsDNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and its Dynamic Associations with the Host Microbiome". Geoffrey Hannigan and Jacquelyn Meisel are gra... | [] |
88,587 | Transfection by electroporation of GFP-LRRK2 and Immunofluorescent imaging of MEFs VPS35 (D620N) mutants stably expressing LysoTag | 4 | dx.doi.org/10.17504/protocols.io.n2bvj3dwxlk5/v1 | https://www.protocols.io/view/transfection-by-electroporation-of-gfp-lrrk2-and-i-c2rjyd4n | Rotimi Y. Fasimoye, Dario R. Alessi | TITLE: Transfection by electroporation of GFP-LRRK2 and Immunofluorescent imaging of MEFs VPS35 (D620N) mutants stably expressing LysoTag
AUTHORS: Rotimi Y. Fasimoye, Dario R. Alessi
[DESCRIPTION]
Transfection of foreign DNA and Immunofluorescent (IF) microscopy are powerful tools used in cellular and molecular biolog... | ["[Transfection of cells with GFP-LRRK2 plasmid by electroporation] Place coverslips in 6 well plate (one coverslip per well) and add 2 mL of media. Place the plate in an incubator.", "[Transfection of cells with GFP-LRRK2 plasmid by electroporation] Pellet cells from 100% confluent 10cm plate and resuspend in 1 mL med... |
80,877 | Protein Extraction from Dental Enamel | 1 | dx.doi.org/10.17504/protocols.io.8epv5j8n4l1b/v1 | https://www.protocols.io/view/protein-extraction-from-dental-enamel-cs8mwhu6 | Alexandra Burnett, simon.daled | TITLE: Protein Extraction from Dental Enamel
AUTHORS: Alexandra Burnett, simon.daled
[DESCRIPTION]
This protocol details a method for extracting proteins from ~5mg of powdered dental enamel for proteomic analysis by LC-MS/MS. This procedure includes the use of alkylating and reducing agents, a sample cleanup step, and... | ["[Sample acquisition] Place your tooth on a fresh piece of weighing paper on a clean worktop. \nUsing a small drill bit, gently abrade the surface of the tooth crown to remove powdered enamel and collect it on the weighing paper.", "[Sample acquisition] Carefully tip the powdered enamel into a protein lo-bind Eppendor... |
17,660 | Circular dichroism | null | dx.doi.org/10.17504/protocols.io.vg4e3yw | null | PALOMA VARELA | TITLE: Circular dichroism
AUTHORS: PALOMA VARELA
[STEPS] | [] |
54,407 | Luminol Calibration | 1 | dx.doi.org/10.17504/protocols.io.bzdfp23n | https://www.protocols.io/view/luminol-calibration-bzdfp23n | Michael Burgis | TITLE: Luminol Calibration
AUTHORS: Michael Burgis
[DESCRIPTION]
Calibration curve using luminol in order to standardize luminescence data
[STEPS]
SECTION: Preparation of the Buffers
1. Preparation of 100ml luminol stock solution (1 mM luminol sodium salt; 50 mM sodium carbonate; 300 mM sodium bicarbonate; 5 mM am... | ["[Preparation of the Buffers] Preparation of 100ml luminol stock solution (1 mM luminol sodium salt; 50 mM sodium carbonate; 300 mM sodium bicarbonate; 5 mM ammonium carbonate) :\ndissolve the following components in ddH2O\n19,94mg of Luminol sodium salt\n529,94mg of sodium carbonate\n2,52g of sodium bicarbonate\n48mg... |
92,032 | Native Barcoding (SQK-NBD114) gDNA for Adaptive Sampling using Oxford Nanopore Technologies | 1 | dx.doi.org/10.17504/protocols.io.kxygx3qx4g8j/v1 | https://www.protocols.io/view/native-barcoding-sqk-nbd114-gdna-for-adaptive-samp-c548y8zw | claire.anderson, Hannah Macpherson, Emil Gustavsson, Jasmaine Lee, zhongbo.chen | TITLE: Native Barcoding (SQK-NBD114) gDNA for Adaptive Sampling using Oxford Nanopore Technologies
AUTHORS: claire.anderson, Hannah Macpherson, Emil Gustavsson, Jasmaine Lee, zhongbo.chen
[DESCRIPTION]
This protocol describes how to carry out native barcoding of genomic DNA (gDNA) using the Native Barcoding Kit 24 V14... | ["[Prepare for your experiment] This protocol is based on ligation-sequencing-gdna-native-barcoding-v14-sqk-nbd114-24-NBE_9169_v114_revF_15Sep2022-promethion with some modifications for adaptive sampling. \n \n\nRefer to the ONT notes on adaptive sampling for further information and creating a .BED file.", "[Prepare s... |
58,873 | Open Field | 4 | dx.doi.org/10.17504/protocols.io.b5qzq5x6 | https://www.protocols.io/view/open-field-b5qzq5x6 | Haley Geertsma | TITLE: Open Field
AUTHORS: Haley Geertsma
[DESCRIPTION]
This protocol is used to test mice in the Open Field behavioural test.
[STEPS]
1. Habituate mice in a separate room for 60 minutes prior to testing.
2. Confirm testing room lighting is set to 100 lux and EthoVision is set up properly.
3. Place mice into open f... | ["Habituate mice in a separate room for 60 minutes prior to testing.", "Confirm testing room lighting is set to 100 lux and EthoVision is set up properly.", "Place mice into open field box and record their movement for 10 minutes.", "When testing is complete, place mice back into their home cage and clean the open fiel... |
32,462 | Mouse Genotyping with KAPA Kit in 2 Hours (#KK7302) | null | dx.doi.org/10.17504/protocols.io.bbxnipme | null | Cq Wu | TITLE: Mouse Genotyping with KAPA Kit in 2 Hours (#KK7302)
AUTHORS: Cq Wu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol uses KAPA Mouse Genotyping kit (#KK7302)</div></div>
[STEPS]
?. [DNA Extraction]
Collect mouse tissues (ear or tail clips) into PCR strips.
?. [DNA Extraction]
Mak... | ["[DNA Extraction]\nCollect mouse tissues (ear or tail clips) into PCR strips.", "[DNA Extraction]\nMake extration buffer mix for each sample. AB110x Extraction buffer10 ul2H2O88 ul3Extraction Enzyme2 ul4Total100 ul\n100 µl\nAB110x Extraction buffer10 ul2H2O88 ul3Extraction Enzyme2 ul4Total100 ul", "[DNA Extraction]\... |
83,648 | Algaeorithm Classroom Guide: Constructing Mini Photobioreactors for Microalgae | 5 | null | https://www.protocols.io/view/algaeorithm-classroom-guide-constructing-mini-phot-cvw8w7hw | Ashwin Mukherjee, rohan chanani, Jacob J. Valenzuela | TITLE: Algaeorithm Classroom Guide: Constructing Mini Photobioreactors for Microalgae
AUTHORS: Ashwin Mukherjee, rohan chanani, Jacob J. Valenzuela
[DESCRIPTION]
A guide to constructing mini photobioreactors and cultivating algae in classroom settings.
[STEPS]
SECTION: Prepare Photobioreactors
1. Place the glass tube... | ["[Prepare Photobioreactors] Place the glass tube rack and unplugged air pump on a table or flat surface where they will not be disturbed.", "[Sample Photobioreactors + Prepare Hemocytometer] Combine 3 parts isopropyl alcohol and 7 parts water in a spray bottle", "[Analyze Samples] Place the 10x eyepieces into the bino... |
80,200 | Sequence analysis of Nanopore based fungal and bacterial metabarcode DNA reads | 5 | null | https://www.protocols.io/view/sequence-analysis-of-nanopore-based-fungal-and-bac-csjgwcjw | Rita.Tam, austin.bird, Benjamin Schwessinger | TITLE: Sequence analysis of Nanopore based fungal and bacterial metabarcode DNA reads
AUTHORS: Rita.Tam, austin.bird, Benjamin Schwessinger
[DESCRIPTION]
This protocols is part of the ANU Biosecurity mini-research project #1 "Plant Pathogen Diagnostics: Visuals, subcultures, and genomics".
You will be provided four... | ["[Data pre-processing] This section gets the raw signal data in the fast5 format into fastq files and filters them down to reasonable junks for you to handle. These reasonable chunks are 10,000 reads for each of your PCR reactions. \n\nAll steps were performed in bash on a Linux Ubuntu LTS 20 workstation.", "[Data pre... |
52,200 | CTAB chloroform DNA extraction from ethanol-preserved filters | 4 | dx.doi.org/10.17504/protocols.io.bw8gphtw | https://www.protocols.io/view/ctab-chloroform-dna-extraction-from-ethanol-preser-bw8gphtw | Dominique L. Chaput | TITLE: CTAB chloroform DNA extraction from ethanol-preserved filters
AUTHORS: Dominique L. Chaput
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol extracts high molecular weight genomic DNA from filters used to sample microbes from aquatic environments. It was developed for polycarbonat... | ["[Preparation]\nSamples collected in the field should be preserved in 100% molecular-grade ethanol, in 2 mL screw-cap cryotubes. Upon arrival at the laboratory, they should be stored at .\n-20 °C\nThis protocol was originally developed for polycarbonate filters, 47 mm diameter, 0.2 μm or 0.4 μm pore size, through whic... |
43,902 | Prevalence of CYP2C19 Polymorphism in Bogotá, Colombia: The first report of allele *17 | 4 | dx.doi.org/10.17504/protocols.io.bn46mgze | https://www.protocols.io/view/prevalence-of-cyp2c19-polymorphism-in-bogot-colomb-bn46mgze | Azucena Arevalo Gavis, William A. Otero-Regino (Unidad de Gastroenterología Facultad de Medicina, Universidad Nacional de Colombia Bogotá D.C., Colombia), Gloria Natalia Ovalle Celis , Eliana Rodriguez, Alba Trespalacios-Rangel | TITLE: Prevalence of CYP2C19 Polymorphism in Bogotá, Colombia: The first report of allele *17
AUTHORS: Azucena Arevalo Gavis, William A. Otero-Regino (Unidad de Gastroenterología Facultad de Medicina, Universidad Nacional de Colombia Bogotá D.C., Colombia), Gloria Natalia Ovalle Celis , Eliana Rodriguez, Alba Trespa... | ["Ethics aprovalThe ethics committees from participant institutes approved the study protocol. Since the study included human samples It was performed in agreement with Good Clinical Practice guidelines and the ethical principles of the Declaration of Helsinki.", "Participants1. Inclusion and exclusion criteria: This ... |
null | null | null | dx.doi.org/10.17504/protocols.io.h7jb9kn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.fa2bige | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocols describes materials needed and steps for maintaining coral larvae in the lab.</p>
[BEFORE_START]
<p>Materials needed:</p>
<p>Glass beakers</p>
<p>Glass bowls</p>
<p>Large glass bowl</p>
<p>Magnifying light</p>
<p>Counter for making larval counts</p>
<p>LED ful... | [] |
35,826 | Detection of SARS-Cov2 Without High Demand Reagents (Singleplex Assays) | null | dx.doi.org/10.17504/protocols.io.be8sjhwe | https://www.protocols.io/view/detection-of-sars-cov2-without-high-demand-reagent-be8sjhwe | Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton | TITLE: Detection of SARS-Cov2 Without High Demand Reagents (Singleplex Assays)
AUTHORS: Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In the United States, access to testing for the novel coronavirus (SARS-Cov2) is seve... | [] |
62,960 | ONT dA-tailing for Fungal Barcoding | 4 | null | https://www.protocols.io/view/ont-da-tailing-for-fungal-barcoding-b9qqr5vw | Stephen Douglas Russell | TITLE: ONT dA-tailing for Fungal Barcoding
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
Tailing is an enzymatic method for adding a non-templated nucleotide to the 3' end of a blunt, double-stranded DNA molecule. This puts A-chains on the end of our PCR product, creating a site for the ligation adapter to attach ... | ["[End repair/A-tailing] In a 0.2mL thin wall, sterile, nuclease-free tube, combine the following in order. Mix each reagent together after it is added by gently pipetting the entire volume up and down 10-20 times for each addition.\n\nIdeal amplicon DNA concentration is 0.5ng per 50mL for Flongle or 1ug DNA per 50uL f... |
20,724 | Tissue lysis and digestion for MS analysis | null | dx.doi.org/10.17504/protocols.io.yguftww | null | David Kotol | TITLE: Tissue lysis and digestion for MS analysis
AUTHORS: David Kotol
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Here, a workflow for sample preparation and quantification of brain and pancreatic tissue proteins with use of heavy labelled protein standards (QPrESTs) is described. The standards... | ["[Protein extraction]\nIncubate adapter of the Tissue Lyser on dry ice for", "[Protein extraction]\nAdd one 3mm bead to the tissue sample and incubate on dry ice for", "[Protein extraction]\nDisrupt the tissue using the Tissue Lyser for", "[Protein extraction]\nAdd of Lysis Buffer (7M Urea, 2M Thiourea, 2% SDC)\n250 µ... |
27,220 | Isolation of single nuclei from postmortem fresh frozen human brain and immunostaining for NeuN | null | dx.doi.org/10.17504/protocols.io.6tuhenw | null | Marcos Otero-Garcia, Inma Cobos | TITLE: Isolation of single nuclei from postmortem fresh frozen human brain and immunostaining for NeuN
AUTHORS: Marcos Otero-Garcia, Inma Cobos
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>- Protocol based on Krishnaswami </span><span style = "font-style:italic;">et al.</span><span>, Nat Pr... | ["Prepare Homogenization Buffer and cool it on ice. ABC1AmountReagentFinal concentration22925 µlIM133 µlDTT 1mM1 µM430 µl50x Protease Inhibitor0.5 x515 µlRNaseIN 40U/µl0.2 U/µl630 µlTritonX100 10 %v/v0.1 %\nABC1AmountReagentFinal concentration22925 µlIM133 µlDTT 1mM1 µM430 µl50x Protease Inhibitor0.5 x515 µlRNaseIN 4... |
46,455 | Primers for amplification of SARS-CoV-2 - 1500bp overlapping amplicons | 3 | dx.doi.org/10.17504/protocols.io.brkxm4xn | https://www.protocols.io/view/primers-for-amplification-of-sars-cov-2-1500bp-ove-brkxm4xn | Leonardo Caserta | TITLE: Primers for amplification of SARS-CoV-2 - 1500bp overlapping amplicons
AUTHORS: Leonardo Caserta
[STEPS] | [] |
21,136 | Electroporation of Thalassiosira pseudonana | null | dx.doi.org/10.17504/protocols.io.yvqfw5w | null | Joshua Bugge, Deborah Robertson | TITLE: Electroporation of Thalassiosira pseudonana
AUTHORS: Joshua Bugge, Deborah Robertson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>An electroporation-mediated genetic transformation or the marine diatom </span><span style = "font-style:italic;">Thalassiosira pseudonana</span><span> wa... | ["Grow cultures of T. psuedonana on f/2 supplemented sterile seawater to a density of approximately 1.1 x 106 cells mL-1", "[Cell collection option 1]\nAll procedures are carried out at 4 C and cells were kept on ice. Collect cells from 500 mL of culture (see step 1) by centrifugation for 10 m in at 3000 x g in 10 x 5... |
25,941 | Preparing Gene of interest for GateWay cloning (2 step PCR process) | null | dx.doi.org/10.17504/protocols.io.5jvg4n6 | null | Johannes Wolfram JWD. Debler | TITLE: Preparing Gene of interest for GateWay cloning (2 step PCR process)
AUTHORS: Johannes Wolfram JWD. Debler
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">GateWay recombination cloning is achieved by flanking your gene of interest with GateWay attachment sites. In our case attB1 and attB2. Tho... | ["[PCR 1 - gene specific PCR]\nUse your favourite High Fidelity Polymerase. I have used NEB Q5 and Thermo Fisher SuperFi Mastermix as listed below with great success. ABCDEF110 ul total25x Q5 Buffer2 ul98°C1 min32 mM dNTPs1 ul98°C10 s|410 uM F primer0.5 ul50°C20 s} 40 x510 uM R primer0.5 ul72°C30 s (per kb)|6Q5 polyme... |
30,716 | Identification of promising genotypes | null | dx.doi.org/10.17504/protocols.io.984h9yw | null | Ding Mingliang, Muhammad Asim, Li Mingju, Sedhom Abdelkhalik, Daniel Manore, Li Shaoxiang, Zhao Hong, Lin Liping | TITLE: Identification of promising genotypes
AUTHORS: Ding Mingliang, Muhammad Asim, Li Mingju, Sedhom Abdelkhalik, Daniel Manore, Li Shaoxiang, Zhao Hong, Lin Liping
[STEPS]
?. Average the yield data generated for genotypes across locations and years.
?. Perform the Welch’s variance-weighted analysis of variance for ... | ["Average the yield data generated for genotypes across locations and years.", "Perform the Welch’s variance-weighted analysis of variance for studied genotypes (across years and locations), at each location (across genotypes and years) and for each year (across genotypes and locations) using One-Way ANOVA model of SAS... |
86,225 | Western blotting using the BioRad Criterion Blotter system | 4 | dx.doi.org/10.17504/protocols.io.j8nlkowy1v5r/v1 | https://www.protocols.io/view/western-blotting-using-the-biorad-criterion-blotte-cyfrxtm6 | Louise Uoselis | TITLE: Western blotting using the BioRad Criterion Blotter system
AUTHORS: Louise Uoselis
[DESCRIPTION]
Protocol for performing an SDS-PAGE Western blot analysis using the BioRad Criterion Blotter system.
[STEPS]
SECTION: Day 1
1. Load samples onto a 4-12% Bis-Tris (NuPage) gel with MOPS buffer (ThermoFisher). If usi... | ["[Day 1] Load samples onto a 4-12% Bis-Tris (NuPage) gel with MOPS buffer (ThermoFisher). If using SDS loading dye (with 5% SDS final concentration), all lanes must contain the same volume of sample. Ensure all lanes are filled with 1x SDS loading dye at the same volumes as the sample/marker lanes. If this is not done... |
42,322 | ANGPTL-protocil-YCU | 3 | dx.doi.org/10.17504/protocols.io.bmjsk4ne | https://www.protocols.io/view/angptl-protocil-ycu-bmjsk4ne | Ty | TITLE: ANGPTL-protocil-YCU
AUTHORS: Ty
[STEPS] | [] |
82,623 | KAT8 compound inhibition inhibits the initial steps of PINK1-dependant mitophagy | 4 | dx.doi.org/10.17504/protocols.io.eq2ly7m8qlx9/v1 | https://www.protocols.io/view/kat8-compound-inhibition-inhibits-the-initial-step-cuw7wxhn | Capucine de Talhouet, Benjamin O'Callaghan, Noemi Esteras Gallego, Helene Plun-Favreau | TITLE: KAT8 compound inhibition inhibits the initial steps of PINK1-dependant mitophagy
AUTHORS: Capucine de Talhouet, Benjamin O'Callaghan, Noemi Esteras Gallego, Helene Plun-Favreau
[DESCRIPTION]
It has recently been shown that KAT8, a genome-wide association study candidate risk gene for Parkinson’s Disease, is inv... | ["[Cell Culture] Maintain cells in culture in a humified 5% CO2 incubator at 37 °C in Dulbecco's modified Eagle's medium (DMEM, Gibco, 11995-065) supplemented with 10% heat-inactivated foetal bovine serum (FBS, Gibco). Change cell medium every 3 days.", "[Cell Culture] Split cells at 75% to 90% confluency using 2 mL of... |
28,710 | Promega Glucose-Glo Assay | null | dx.doi.org/10.17504/protocols.io.8aehsbe | null | NUS iGEM | TITLE: Promega Glucose-Glo Assay
AUTHORS: NUS iGEM
[STEPS]
?. Prepare glucose detection reagent mix by adding: Luciferin Detection Solution Reductase Reductase Substrate Glucose Dehydrogenase NAD
These reagents are part of a boxed set - Promega's Glucose-Glo Assay.
?. Dilute overnight culture in 1x PBS and mix
2 µl
... | ["Prepare glucose detection reagent mix by adding: Luciferin Detection Solution Reductase Reductase Substrate Glucose Dehydrogenase NAD\nThese reagents are part of a boxed set - Promega's Glucose-Glo Assay.", "Dilute overnight culture in 1x PBS and mix\n2 µl\n198 µl", "Aliquot of each sample into three wells of 96-w... |
59,358 | Quantitative immunoblot analysis of LRRK1 signalling pathway | 4 | dx.doi.org/10.17504/protocols.io.6qpvr68e3vmk/v1 | https://www.protocols.io/view/quantitative-immunoblot-analysis-of-lrrk1-signalli-b576q9re | Asad Malik, Athanasios Karapetsas, Francesca Tonelli , Dario R Alessi | TITLE: Quantitative immunoblot analysis of LRRK1 signalling pathway
AUTHORS: Asad Malik, Athanasios Karapetsas, Francesca Tonelli , Dario R Alessi
[DESCRIPTION]
Accurate, quantitative analysis of protein expression and modifications (such as phosphorylation) is critical when studying cell signalling. Here we describ... | ["[Preparation of lysates from cultured cells] Quickly rinse cells in the tissue culture dish by carefully pouring 4 Room temperature culture media without Foetal bovine serum (FBS) into the dish.", "[Preparation of lysates from cultured cells] Pour off media from the culture dish and completely aspirate any residual m... |
43,593 | Elution, SDS-PAGE, and RNA Purification | 4 | dx.doi.org/10.17504/protocols.io.bnthmej6 | https://www.protocols.io/view/elution-sds-page-and-rna-purification-bnthmej6 | Clémentine Delan-Forino, David Tollervey | TITLE: Elution, SDS-PAGE, and RNA Purification
AUTHORS: Clémentine Delan-Forino, David Tollervey
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The RNA exosome complex functions in both the accurate processing and rapid degradation of many classes of RNA in eukaryotes and Archaea. Functional and st... | ["[Elution and Precipitation of Exosome Subunit–RNA Complexes]\nSpin out the void volume. Close the bottom of the column with the press-on stopper and add .\n[Elution buffer]", "[Elution and Precipitation of Exosome Subunit–RNA Complexes]\nIncubate the resin for .\non ice\nAlternatively, to increase efficiency, incuba... |
78,716 | ONT Flongle Flowcell Loading with Q20+ (V14) Chemistry | 4 | dx.doi.org/10.17504/protocols.io.ewov1nm5pgr2/v4 | https://www.protocols.io/view/ont-flongle-flowcell-loading-with-q20-v14-chemistr-cq44vyyw | Stephen Douglas Russell | TITLE: ONT Flongle Flowcell Loading with Q20+ (V14) Chemistry
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
Overview: This protocol describes the steps used to load a 10.4.1 Flongle flowcell utilizing the Q20+ (V14) Ligation Sequencing Kit from ONT.
This protocol has been tested with Flongle R10.4.1 flowcells... | ["[Starting out] Before starting, watch the first 13 minutes of this video: https://vimeo.com/651243660\n\nIt covers all of the vital aspects of this protocol.", "[Starting out] Begin by restarting your computer. This will help to ensure there are no performance issues during your run or other programs running that may... |
null | null | null | dx.doi.org/10.17504/protocols.io.ug5ety6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Depending on tissue and analytes that will be analysed different matrices can be used. The following 2 matrices are first steps to analyse lipids (SDHB) and peptides (CHCA). Matrix concentration and solvent ratio as well as instrument settings can be adjusted specifically to tis... | ["Open the SunCollect Software V1.7.56.", "Open the Nitrogen Flow to 2 bar.", "Take the 1 ml syringe out of the syringe pump, fill it with cleaning solution and mount the syringe again to the syringe pump.", "Select a flow of 20 µl/min at the syringe pump settings and start the flow. Check if droplets are coming out at... |
35,619 | Opentrons COVID-19 testing (Zymo, station B, 24 samples) | null | dx.doi.org/10.17504/protocols.io.be2bjgan | https://www.protocols.io/view/opentrons-covid-19-testing-zymo-station-b-24-sampl-be2bjgan | Max Marrone | TITLE: Opentrons COVID-19 testing (Zymo, station B, 24 samples)
AUTHORS: Max Marrone
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Station B: Initial OT-2 setup]
Clean the OT-2.
?. [Station B: Initial OT-2 setup]
Place the following labware on the OT-2's deck:Slot 1: An Opentrons Temperature Module wit... | ["[Station B: Initial OT-2 setup]\nClean the OT-2.", "[Station B: Initial OT-2 setup]\nPlace the following labware on the OT-2's deck:Slot 1: An Opentrons Temperature Module with an Opentrons 96 well aluminum block and an empty, sterile NEST 100 µL PCR plate.Slots 3 and 6: A full, sterile rack of Opentrons 200 µL filte... |
90,547 | Short amplicons panels (Artic-like) for RSVA and RSVB | 4 | dx.doi.org/10.17504/protocols.io.eq2lyj1nrlx9/v1 | https://www.protocols.io/view/short-amplicons-panels-artic-like-for-rsva-and-rsv-c4ntyven | Flora Donati, matthieu.prot, Banujaa Jeyarajah, etienne.simon-loriere | TITLE: Short amplicons panels (Artic-like) for RSVA and RSVB
AUTHORS: Flora Donati, matthieu.prot, Banujaa Jeyarajah, etienne.simon-loriere
[DESCRIPTION]
This SOP describes the procedure for generating cDNA from respiratory syncytial virus A or B (RSVA or RSVB) viral nucleic acid extracts and subsequently producing ~4... | ["[cDNA preparation] In a mastermix cabinet, mix the following components:\n\nComponent Volume\nLunaScript Master Mix 2 µL \nTemplate RNA 8 µL \n\nMix by pipetting gently and spin down the tube.", "[cDNA preparation] Incubate as follows:\n\n25 °C for 2 min \n55 °C for 20 min \n9... |
null | null | null | dx.doi.org/10.17504/protocols.io.geubtew | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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21,778 | Criminal Justice Policy Document Analysis . Version 2 | null | dx.doi.org/10.17504/protocols.io.zhsf36e | null | Drew Gitomer | TITLE: Criminal Justice Policy Document Analysis . Version 2
AUTHORS: Drew Gitomer
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is an adaptation of Yanovitzky & Weber - because we were interested adapting Yanovitzky and Weber's tool for criminal justice reform.
All of the policymaking pro... | ["[Interviews of Congressional Staff]\nWhat information influenced your position on childhood obesity legislation?", "[Interviews of Congressional Staff]\nHow did you obtain that information?", "[Interviews of Congressional Staff]\nHow did you evaluate that information?"] |
105,751 | Titration of Lentivirus | 4 | dx.doi.org/10.17504/protocols.io.14egn6yj6l5d/v1 | https://www.protocols.io/view/titration-of-lentivirus-djhx4j7n | Anita Adami | TITLE: Titration of Lentivirus
AUTHORS: Anita Adami
[DESCRIPTION]
This protocol is about titrating lentivirus.
[STEPS]
SECTION: Virus transduction
1. When aliqouting the virus, prepare 1 vial with 6 μl for titration. This vial must go through one cycle of freeze / thaw before titration and if you want to titer in con... | ["[Virus transduction] When aliqouting the virus, prepare 1 vial with 6 μl for titration. This vial must go through one cycle of freeze / thaw before titration and if you want to titer in conjunction with aliqouting you can put the vials on dry-ice for a bit.", "[Virus transduction] Seed cells in 6 well plates seed 100... |
50,125 | Serial Sectioning Optical Histology - Cell Census Network -data acquisition and processing | 3 | null | https://www.protocols.io/view/serial-sectioning-optical-histology-cell-census-ne-bu7mnzk6 | shuaibin Chang, Jiarui Yang | TITLE: Serial Sectioning Optical Histology - Cell Census Network -data acquisition and processing
AUTHORS: shuaibin Chang, Jiarui Yang
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">No current imaging technology can directly and without significant distortion visualize the defining microscopic fea... | [] |
63,383 | Hazel Hills CBD Gummies | 3 | dx.doi.org/10.17504/protocols.io.n92ldzjbov5b/v1 | https://www.protocols.io/view/hazel-hills-cbd-gummies-b95xr87n | NanicLews | TITLE: Hazel Hills CBD Gummies
AUTHORS: NanicLews
[DESCRIPTION]
Hazel Hills CBD Gummies its 100% effective & beneficial for reducing stress, pain & anxiety {buy now on official website}
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.dim4c5 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
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80,139 | Determination of NM Concentration | 1 | dx.doi.org/10.17504/protocols.io.n2bvj8wxxgk5/v1 | https://www.protocols.io/view/determination-of-nm-concentration-cshjwb4n | Xiqun Chen | TITLE: Determination of NM Concentration
AUTHORS: Xiqun Chen
[DESCRIPTION]
This is the protocol for determining neuromelanin concentration and data.
[STEPS]
1. Place SNpc tissue in a plastic tube and carefully ground it. Weigh 10 mg of tissue for each sample, and place itin a 5 mL glass tube.
2. In each tube, add 1.5... | ["Place SNpc tissue in a plastic tube and carefully ground it. Weigh 10 mg of tissue for each sample, and place itin a 5 mL glass tube.", "In each tube, add 1.5 mL of pH 7.4 phosphate buffer (50mM), shake, and centrifuge at 9000 x g for 30 mins. Discard the supernatant.", "Wash with phosphate buffer and repeat once mor... |
42,573 | Kidney biopsy findings in vancomycin-induced acute kidney injury: a pooled analysis | 1 | dx.doi.org/10.17504/protocols.io.bmtmk6k6 | https://www.protocols.io/view/kidney-biopsy-findings-in-vancomycin-induced-acute-bmtmk6k6 | Ioannis Bellos | TITLE: Kidney biopsy findings in vancomycin-induced acute kidney injury: a pooled analysis
AUTHORS: Ioannis Bellos
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Vancomycin represents a tricyclic glycopeptide that is widely administered to patients with Gram-positive infections. Acute kidney injury... | ["Background: Vancomycin represents a tricyclic glycopeptide that is widely administered to patients with Gram-positive infections. Acute kidney injury is considered as an important adverse effect, as it commonly leads to significant morbidity and high rates of treatment discontinuation. Vancomycin has been suggested t... |
92,774 | Environmental DNA (eDNA) 12S Metabarcoding PCR Protocol (with Platinum SuperFi II Taq) | 1 | null | https://www.protocols.io/view/environmental-dna-edna-12s-metabarcoding-pcr-proto-c6uezete | Kathleen Pitz, Jacoby Baker | TITLE: Environmental DNA (eDNA) 12S Metabarcoding PCR Protocol (with Platinum SuperFi II Taq)
AUTHORS: Kathleen Pitz, Jacoby Baker
[DESCRIPTION]
The 12S protocol is aimed at amplifying the hypervariable region of the mitochondrial DNA 12S rRNA gene in eukaryotes. The primers (MiFish-U-F & MiFish-U-R) used in this pro... | ["[MIOP: Minimum Information about an Omics Protocol] MIOP Term\nValue\n \nmethodology category\n\n \nproject\n\n \npurpose\n\n \nanalyses\n\n \ngeographic location\n\n \nbroad-scale environmental context\n\n \nlocal environmental context\n\n \nenvironmental medium\n\n \ntarget\n\n \ncreator\n\n \nmaterials required\... |
29,886 | Cell culture | 1 | null | https://www.protocols.io/view/cell-culture-9e6h3he | Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying | TITLE: Cell culture
AUTHORS: Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol explains the cell culture and characterizatio... | ["[Cell Culture]\nObtain human iPSC lines from Coriell Cell Repositories or through reprogramming patient cells.", "[Cell Culture]\nCharacterize pluripotency of iPSCs by immunochemistry for pluripotent cell markers (NANOG, OCT4, TRA‐1‐60, and SSEA‐3) and through embryoid body formation assay.", "[Cell Culture]\nMaintai... |
99,107 | BrainSaw 50 mM pH 7.4 PB slicing buffer | 0 | dx.doi.org/10.17504/protocols.io.q26g714r3gwz/v1 | https://www.protocols.io/view/brainsaw-50-mm-ph-7-4-pb-slicing-buffer-dc2b2yan | Rob Campbell | TITLE: BrainSaw 50 mM pH 7.4 PB slicing buffer
AUTHORS: Rob Campbell
[DESCRIPTION]
This protocol makes 2L of pH 7.4 phosphate buffer. This solution is used for serial section 2-photon imaging.
[STEPS]
1. Fill 2L flask with MQ water to the 2L notch. Place on magnetic stirrer and set to 1000 RPM.
2. Weigh 3.1 g of ... | ["Fill 2L flask with MQ water to the 2L notch. Place on magnetic stirrer and set to 1000 RPM.", "Weigh 3.1 g of and pour into the flask.", "Weigh out 20.8 g of but do not pour into flask until existing solute is dissolved.", "Wait until has dissolved. Skipping this step will result in a small quantity of fine p... |
null | null | null | dx.doi.org/10.17504/protocols.io.smwec7e | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.rd4d28w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>There has been an increased use of medical <em>Cannabis</em> in the United States of America as more states legalize its use. Complete chemical analyses of this material can vary considerably between producers and is often not fully provided to consumers. We report the devel... | [] |
67,444 | https://www.facebook.com/PrimaDietpillsDragonsDenUK/ | 1 | dx.doi.org/10.17504/protocols.io.kqdg3pj5pl25/v1 | https://www.protocols.io/view/https-www-facebook-com-primadietpillsdragonsdenuk-cd4us8ww | alexballosz | TITLE: https://www.facebook.com/PrimaDietpillsDragonsDenUK/
AUTHORS: alexballosz
[DESCRIPTION]
Prima Weight Loss Dragons Den UK
Prima Weight Loss Dragons Den UK is a company that helps people who are interested in using their fitness knowledge to make more money. They offer an online blog and video courses, which pr... | ["[https://www.facebook.com/PrimaDietpillsDragonsDenUK/]"] |
34,551 | Measuring PPFD on Algal Shaker | null | dx.doi.org/10.17504/protocols.io.bdyxi7xn | null | Jakub Nedbal | TITLE: Measuring PPFD on Algal Shaker
AUTHORS: Jakub Nedbal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The illuminated orbital shaker features a glowing growing area. The illumination is not entirely homogeneous. It is brightest near the centers of the LEDs and becomes dimmer closer to the edge... | ["[Preparation]\nSwitch off the algal shakerClear out any culture flasks from the algal shaker.Move it onto a bench.", "[Preparation]\nWork in a dark room. Turn all lights in the room off. Keep them off during the measurements.", "[Preparation]\nConnect the Walz probe to the Li-COR Light Meter.Set the Li-COR Light Mete... |
34,984 | Trypan blue and Turk solution | null | dx.doi.org/10.17504/protocols.io.beegjbbw | https://www.protocols.io/view/trypan-blue-and-turk-solution-beegjbbw | Marco Cosentino, Elisa Storelli, Alessandra Luini, Emanuela Rasini, Massimiliano Legnaro, Marco Ferrari, Franca Marino | TITLE: Trypan blue and Turk solution
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Emanuela Rasini, Massimiliano Legnaro, Marco Ferrari, Franca Marino
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Trypan Blue test</span></div><div class = "text-block... | [] |
45,621 | Delay gesture experiment | 1 | dx.doi.org/10.17504/protocols.io.bqsvmwe6 | https://www.protocols.io/view/delay-gesture-experiment-bqsvmwe6 | Federica Cavicchio | TITLE: Delay gesture experiment
AUTHORS: Federica Cavicchio
[STEPS]
?. | [] |
73,932 | GenomeTrakr WGS Protocol Collection and Workflow for MiSeq | 1 | dx.doi.org/10.17504/protocols.io.3byl4bwyjvo5/v2 | https://www.protocols.io/view/genometrakr-wgs-protocol-collection-and-workflow-f-ckfkutkw | Tina.Pfefer, Julie Haendiges, Maria Balkey, Ruth Timme | TITLE: GenomeTrakr WGS Protocol Collection and Workflow for MiSeq
AUTHORS: Tina.Pfefer, Julie Haendiges, Maria Balkey, Ruth Timme
[DESCRIPTION]
Here we have created a collection of all the protocols used for WGS using the MiSeq, in order, from sample extraction to NCBI submission.
This collection has three sections... | ["[Wet lab] WGS Wet lab workflow for Illumina MiSeq", "[Wet lab] DNA Extraction", "[Wet lab] DNA Quantification", "[Wet lab] Library Preparation", "[Wet lab] Sequencing", "[Dry lab - Direct Submission] Dry lab workflow for sequence QC and NCBI submission - Direct Submission: \n\n\nThe following protocols are also inclu... |
28,916 | Hybridization of DNA oligos | null | dx.doi.org/10.17504/protocols.io.8guhtww | null | Laura Sánchez | TITLE: Hybridization of DNA oligos
AUTHORS: Laura Sánchez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to hibridate two complementary DNA chains.</div></div>
[STEPS]
?. [Annealing of oligonucleotides]
Add the required volume of H2O to the lyophilized oligonucleotides to obt... | ["[Annealing of oligonucleotides]\nAdd the required volume of H2O to the lyophilized oligonucleotides to obtain a concentration of 100 μM. Vortex both tubes for 30 s and incubate them at RT for 5 min to dissolve them.", "[Annealing of oligonucleotides]\nPrepare the annealing mix by adding into a PCR tube:> 45.5 μl of ... |
41,829 | COVIDscanDX LAMP | 1 | dx.doi.org/10.17504/protocols.io.bk4dkys6 | https://www.protocols.io/view/covidscandx-lamp-bk4dkys6 | drdon | TITLE: COVIDscanDX LAMP
AUTHORS: drdon
[STEPS]
?. [Specimen Swab Collection]
Use a flocked tapered swab. Tilt head back 70 degrees. While gently rotating the swab, insert swab less than one inch (about 2 cm) into nostril (until resistance is met at turbinates). Rotate the swab several times against nasal wall and rep... | ["[Specimen Swab Collection]\nUse a flocked tapered swab. Tilt head back 70 degrees. While gently rotating the swab, insert swab less than one inch (about 2 cm) into nostril (until resistance is met at turbinates). Rotate the swab several times against nasal wall and repeat in other nostril using the same swab.", "[Spe... |
45,084 | nPCR measurement method | 3 | dx.doi.org/10.17504/protocols.io.bp94mr8w | https://www.protocols.io/view/npcr-measurement-method-bp94mr8w | lyzhaojh | TITLE: nPCR measurement method
AUTHORS: lyzhaojh
[STEPS] | [] |
58,087 | Primary astrocyte culture | 4 | dx.doi.org/10.17504/protocols.io.b4yfqxtn | https://www.protocols.io/view/primary-astrocyte-culture-b4yfqxtn | Xiqun Chen, Qing Ye | TITLE: Primary astrocyte culture
AUTHORS: Xiqun Chen, Qing Ye
[DESCRIPTION]
Primary astrocytes were obtained from C57BL/6 mice embryonic day 17. The dissected cortical tissue was digested, triturated, and centrifuged. The cell pellet was resuspended in high-glucose DMEM/F12 supplemented with 10% FBS. Cells were seeded... | ["For primary astrocyte culture - Use C57BL/6J mice at embryonic day 17", "Anesthetized pregnant mice (1% sodium pentobarbital, 80mg/kg), dissect their embryos and collect the cortex. \n(Separate and remove the soft membrane and blood vessels, rinse the cerebral cortex in PBS, and use the ophthalmic scissor to cut piec... |
null | null | null | dx.doi.org/10.17504/protocols.io.jv7cn9n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Modified from Bigelow: NCMAM Center. Components to prepare 1 L of f/2 vitamin solution.</p>
[BEFORE_START]
<p>Prepare primary stock solutions (in dH<sub>2</sub>O) of the following:</p>
<ul>
<li>biotin 0.1 g/L</li>
<li>cyanocobalamin: 1.0 g/L</li>
</ul>
<p>Filter sterilize th... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ibkcakw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) or Maddie Denney (mdenney5@vols.utk.edu) for additional information regarding this protocol.</p>
<p> </p>
<p>Modified from Guan, Riezman, Wenk & Riezman, 2010</p>
<p> </p>
<p>Please note that there are two versions of th... | [] |
72,495 | Extraction and ONT MinION Library Preparation of uHMW gDNA | 4 | dx.doi.org/10.17504/protocols.io.j8nlkww11l5r/v3 | https://www.protocols.io/view/extraction-and-ont-minion-library-preparation-of-u-ci2pugdn | Kaylee S. Herzog, jfauver | TITLE: Extraction and ONT MinION Library Preparation of uHMW gDNA
AUTHORS: Kaylee S. Herzog, jfauver
[DESCRIPTION]
This custom protocol optimizes extraction, purification, and Oxford Nanopore Technologies (ONT) MinION library preparation for ultra-high molecular weight genomic DNA (uHMW gDNA) from parasitic nematodes.... | ["[Part 1: Ultra-HWM gDNA extraction | Zymo Quick-DNA HWM MagBead Plus Kit | ~3 hr] Set dry bath to 55 °C", "For each sample, add the following to a clean 1.5 mL microcentrifuge tube to create a master mix:\n 95 µL \n 95 µL \n 10 µL", "Vortex the master mix gently to mix, then spin down and keep ... |
62,556 | Approved Science Keto Reviews - Is It legitimate Or Fake? | 1 | dx.doi.org/10.17504/protocols.io.kxygxzo2ov8j/v1 | https://www.protocols.io/view/approved-science-keto-reviews-is-it-legitimate-or-b9b4r2qw | approvedsciencereviews | TITLE: Approved Science Keto Reviews - Is It legitimate Or Fake?
AUTHORS: approvedsciencereviews
[DESCRIPTION]
Approved Science Keto Reviews - Is It legitimate Or Fake?
[STEPS]
1. Approved Science Keto Reviews - Is It legitimate Or Fake?
Currently everyone loves junk food and some people are consuming lots of unhe... | ["Approved Science Keto Reviews - Is It legitimate Or Fake?\nCurrently everyone loves junk food and some people are consuming lots of unhealthy food constantly. But this habit can take you towards rotundity and it's the problem which is being faced by millions in the USAalone.However, also we've an effective product fo... |
null | null | null | dx.doi.org/10.17504/protocols.io.cy7xzm | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<strong>MATERIALS<br /><br />Medium</strong>: NO LIF ES medium<br /><strong>Dish</strong>: BD Petri Dish.<br />Trypsin<br />4% PFA <br />PBS
[STEPS]
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86,562 | Mouse colon vasculature labeling combined with immunofluorescence | 1 | dx.doi.org/10.17504/protocols.io.e6nvwddd7lmk/v1 | https://www.protocols.io/view/mouse-colon-vasculature-labeling-combined-with-imm-cysaxwae | Lixin Wang | TITLE: Mouse colon vasculature labeling combined with immunofluorescence
AUTHORS: Lixin Wang
[DESCRIPTION]
To study the distribution, morphology and innervation of vasculature in different mouse colonic segments and layers, as well as spatial relationships of the vasculature with the enteric plexuses, glia and macroph... | ["[Tissue collection and preparation]", "[Vasculature painting]", "[Vasculature painting] Dilute WGA AF-488 in 0.1M phosphate-buffer saline (PBS) at 30 µg/ml.", "[Vasculature painting] Anesthetized mice deeply with 5% isoflurane.", "[Tissue collection and preparation] Remove the whole colon is removed from the ileoceca... |
null | null | null | dx.doi.org/10.17504/protocols.io.dik4cv | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
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null | null | null | dx.doi.org/10.17504/protocols.io.hiub4ew | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A description of how samples are collected and processed as part of MLML Smith Lab's weekly HAB monitoring efforts in Monterey, CA.</p>
<ul>
<li>Sampling Location: Monterey Commercial Wharf, Monterey, CA. 36° 36.3' N 121° 53.3' W</li>
<li>Oceanographic and meteorological obse... | [] |
9,361 | Transformation of Synechocystis sp. PCC 6803 | null | dx.doi.org/10.17504/protocols.io.mdrc256 | null | Anika Wiegard, Ilka Maria Axmann | TITLE: Transformation of Synechocystis sp. PCC 6803
AUTHORS: Anika Wiegard, Ilka Maria Axmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes how to transform naturally competent </span><span style = "font-style:italic;">Synechocystis</span><span> sp. PCC 6803.</span></... | ["Grow Synechocystis sp. PCC 6803 cells in BG-11 medium (see e.g. Köbler and Wilde https://dx.doi.org/10.17504/protocols.io.wj5fcq6 for recipe) until OD750 nm =~0.5 -1\nshould take about 4 days after inoculation", "Transfer 10 ml of the cell suspension to a sterile 15 ml falcon tube and spin down at ~ 2 200 rcf at room... |
60,953 | Protocolo Tcg4 | 4 | dx.doi.org/10.17504/protocols.io.8epv59nq5g1b/v1 | https://www.protocols.io/view/protocolo-tcg4-b7rzrm76 | dgalvana | TITLE: Protocolo Tcg4
AUTHORS: dgalvana
[DESCRIPTION]
Protocolo para realizar PCR de Tcg4
[STEPS]
SECTION: Protocolo de Tcg4
1. NOTA: Antes de comenzar es recomendable preparar la reacción sobre hielo. Todos los materiales deben estar estériles y limpios. Todo el procedimiento debe ser llevado a cabo dentro de la c... | ["[Protocolo de Tcg4] NOTA: Antes de comenzar es recomendable preparar la reacción sobre hielo. Todos los materiales deben estar estériles y limpios. Todo el procedimiento debe ser llevado a cabo dentro de la campana negra (previamente limpia con solución agua: cloro y esterilizada con UV durante 15 min)", "[Protocolo ... |
78,434 | Indirect immunofluorescence - tissue staining in TMA and whole tissue FFPE sections | 4 | null | https://www.protocols.io/view/indirect-immunofluorescence-tissue-staining-in-tma-cquavwse | Anna Martinez Casals, Nicholas Mitsios, Jan Mulder, Emma Lundberg | TITLE: Indirect immunofluorescence - tissue staining in TMA and whole tissue FFPE sections
AUTHORS: Anna Martinez Casals, Nicholas Mitsios, Jan Mulder, Emma Lundberg
[DESCRIPTION]
Immunofluorescence staining allows detection and localization of antigens in different tissue types providing high sensitivity. The indirec... | ["[Tissue preparation] Place the microscope slide (tissue facing up) in a slide warmer and bake it at 55 °C \n during 60 min.", "[Staining day 2] Take an aliquot of TNB buffer and leave it at RT.", "[Tissue preparation] Label the slide.", "[Tissue preparation] Place the microscope slide in a staining rack and let it co... |
null | null | null | dx.doi.org/10.17504/protocols.io.sh6eb9e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p> </p>
<p> <strong> </strong></p>
[BEFORE_START]
<p><strong>Medium recipe:</strong></p>
<p>ATCC medium: 802 Sonneborn's Paramecium medium Solution 1 Rye grass Cerophyll:</p>
<p>Cerophyll*...................2.5 g</p>
<p>Distilled water..............1.0 L</p>
<p>Add cerophyll t... | [] |
61,964 | Labelled APIs | 6 | dx.doi.org/10.17504/protocols.io.5qpvobq8zl4o/v1 | https://www.protocols.io/view/labelled-apis-b8rkrv4w | BOC Sciences | TITLE: Labelled APIs
AUTHORS: BOC Sciences
[DESCRIPTION]
Isotope-labeled API is a chemical reagent that contains one or several isotopic atoms in the molecule, and uses its traceability for analytical determination. The chemical properties of the reagents replaced by isotopes are usually unchanged except for isot... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.j9mcr46 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">4ヵ月齢のヌードマウスの歩行。 </span></span></p>
[STEPS]
?. | [] |
15,866 | Manual dissection of the Schistosoma mansoni esophagus and back end for proteomic analysis | null | dx.doi.org/10.17504/protocols.io.tq2emye | null | Leandro Neves, R. Alan Wilson, William de Castro Borges | TITLE: Manual dissection of the Schistosoma mansoni esophagus and back end for proteomic analysis
AUTHORS: Leandro Neves, R. Alan Wilson, William de Castro Borges
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Schistosomes are intravenous parasites with ability to survive in the mammalian hos... | ["[Place the Petri dish with worms on the microscope]\nTake the Petri dish out of the ice bath, touch it against a paper to remove the excess of water, place it under the stereomicroscope and adjust the focus.", "[Zoom in at the head of a male worm]\nLook for a male parasite with uncurled head/esophagus. Increase the l... |
47,715 | Basic Analysis Protocol | 5 | null | https://www.protocols.io/view/basic-analysis-protocol-bsubnesn | Clark Fritsch | TITLE: Basic Analysis Protocol
AUTHORS: Clark Fritsch
[DESCRIPTION]
This protocol is meant to describe the basic procedure needed to go from .nd2 files that are recorded using NIS-ELEMENTS during a standard single-molecule FRET experiment to usable FRET time traces that can be used for further downstream analysis.
[S... | ["In NIS-Elements, we record our single-molecule FRET movies with a .nd2 file format. However, to analyze our data we must first convert our movies from .nd2 files to .tiff files.", "We can convert .nd2 files to .tiff files in several ways, depending on whether you used alternating laser excitation (ALEX) during your m... |
46,795 | Human Brain Vascular Pericytes (HBVP) Culture and Plating | 4 | dx.doi.org/10.17504/protocols.io.brxjm7kn | https://www.protocols.io/view/human-brain-vascular-pericytes-hbvp-culture-and-pl-brxjm7kn | Rayan Khaddaj, Maxime Bernad, Daniel Manrique-Castano | TITLE: Human Brain Vascular Pericytes (HBVP) Culture and Plating
AUTHORS: Rayan Khaddaj, Maxime Bernad, Daniel Manrique-Castano
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol implements the culture of Human Brain Vascular Pericytes (HBVP) and is suitable for several research technique... | ["[Cell Culture]\nPrepare and warm of Pericyte Complete Medium (PCM, see materials section) at for .\n50 mL\n37 °C", "[Cell Culture]\nRetrieve form the Human Brain Vascular Pericytes (HBVP) and place them at for .\n-130 °C\n37 °C\nAs frozen HBVP contain DMSO, cell exposure to this reagent at should be minimal.... |
94,198 | Object Location Test | 4 | dx.doi.org/10.17504/protocols.io.rm7vzxdo4gx1/v1 | https://www.protocols.io/view/object-location-test-c78wzrxe | Lisa Blackmer-Raynolds, Ian N Krout, Tim Sampson | TITLE: Object Location Test
AUTHORS: Lisa Blackmer-Raynolds, Ian N Krout, Tim Sampson
[DESCRIPTION]
The object location test is a spatial recognition memory test used to assess cognitive function in rodents. It is based on a rodent’s natural preference to explore objects in a novel location over objects in a location ... | ["[Schematic Overview] Testing begins with a 10 min habituation phase that allows the mice to acclimate to the testing environment (note, this can also serve as an open field test to assess anxiety and locomotor behavior). On the following day, OLT testing—consisting of two phases—begins. During the initial study phase... |
90,142 | Village Nuclei Isolation With Optiprep | 4 | dx.doi.org/10.17504/protocols.io.36wgq3bmxlk5/v1 | https://www.protocols.io/view/village-nuclei-isolation-with-optiprep-c396yr9e | liv_spina, Tara McDonald, Nora Reed, Alyssa Lutservitz | TITLE: Village Nuclei Isolation With Optiprep
AUTHORS: liv_spina, Tara McDonald, Nora Reed, Alyssa Lutservitz
[DESCRIPTION]
Isolation of nuclei from fresh-frozen brain tissue from sets of multiple (typically 2-20) human donors for analysis as a “cell village” (Wells et al., PMID 36796362) in which nuclei from all dono... | ["[Before Starting] Gather Supplies\nScalpels\nGlass slides\n14 mL Dounce\n20 µm vacuum filter\nEppendorf tubes (1.5 mL and 5 mL)\nEppendorf or Rainin pipette tips\nDry ice\nMetal plate\nOCT (Optimal cutting temperature compound)\nRNAse free water\nCell counting supplies (LUNA-FL)\nOther Reagents:\nPBS\nBSA\nRNAse inhi... |
null | null | null | dx.doi.org/10.17504/protocols.io.r9id94e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Third generation sequencing aims to sequence long DNA molecules. High-quality and high-molecular-weight DNA is needed to fully benefit from the potential of third-generation sequencers. The cost of sequencing continues to decrease but DNA extraction kits remain expensive. Her... | [] |
63,923 | Cell Apoptosis Assay | 6 | dx.doi.org/10.17504/protocols.io.eq2lyn2mevx9/v1 | https://www.protocols.io/view/cell-apoptosis-assay-cantsden | BOC Sciences | TITLE: Cell Apoptosis Assay
AUTHORS: BOC Sciences
[DESCRIPTION]
Understanding the mechanism of cell death and survival is the key content of toxicological analysis and drug development and application. Since the cell death pathway is complex and dynamic, multi-parameter analysis is very important for the accurat... | [] |
23,018 | U Cinn - Body Composition & Carcass Analysis | null | dx.doi.org/10.17504/protocols.io.2qigdue | null | Patrick Tso, Dana Lee | TITLE: U Cinn - Body Composition & Carcass Analysis
AUTHORS: Patrick Tso, Dana Lee
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Total body composition in live, un-anaesthetized small animals and carcasses will revea... | ["Insert the calibration tube into opening on right side of the EchoMRI-100 as far in as possible.", "Select “Calibrate” at the bottom of the screen to calibrate the system.", "After calibration has passed, weigh the animal and carefully place in the restrainer tube.", "Insert the restrainer tube into the opening on th... |
31,084 | Immunohistochemical labelling of spinal cord neurons involved in bladder activity | null | dx.doi.org/10.17504/protocols.io.bakkicuw | https://www.protocols.io/view/immunohistochemical-labelling-of-spinal-cord-neuro-bakkicuw | Janet Keast, Peregrine Osborne, Nicole Wiedmann | TITLE: Immunohistochemical labelling of spinal cord neurons involved in bladder activity
AUTHORS: Janet Keast, Peregrine Osborne, Nicole Wiedmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used for immunohistochemical visualisation of immediate early gene expression (c-Fos or E... | ["[Preparation of cryosections]\nCryoprotect fixed tissue (L5-S2 spinal cord) in phosphate-buffered saline (PBS; 0.1 M, pH7.2) containing 30% sucrose. This should be performed at 4C, 24-72h prior to cutting.", "[Preparation of cryosections]\nEmbed tissue in cryomold using OCT, freeze in cryostat and cut sections (40 µm... |
60,659 | Mod3D Live Cell Chambers and holders 3D printing and Assembly | 1 | dx.doi.org/10.17504/protocols.io.b7gtrjwn | https://www.protocols.io/view/mod3d-live-cell-chambers-and-holders-3d-printing-a-b7gtrjwn | and Ray Truant | TITLE: Mod3D Live Cell Chambers and holders 3D printing and Assembly
AUTHORS: and Ray Truant
[DESCRIPTION]
Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However... | ["For FDM prints of holders, print at 20% grid infill with a layer height of 0.16 mm on either a Creality Ender 3 or a CR10 printer (Creality, Shenzhen, China) or similar FDM printer. Poly lactic acid (PLA) or Polyethylene terephthalate glycol (PETG) 1.75mm filament should be used. Acrylonitrile butadiene styrene (ABS)... |
62,240 | Optimum Max Keto Pills - Does It Have Side Effects? | 1 | dx.doi.org/10.17504/protocols.io.5qpvob7n9l4o/v1 | https://www.protocols.io/view/optimum-max-keto-pills-does-it-have-side-effects-b8z8rx9w | optimummaxpillseffects | TITLE: Optimum Max Keto Pills - Does It Have Side Effects?
AUTHORS: optimummaxpillseffects
[DESCRIPTION]
Optimum Max Keto Pills - Does It Have Side Effects?
[STEPS]
1. Optimum Max Keto Pills - Does It Have Side Effects?
OPTIMAL MAX KETO REVIEWS
Optimal Max Keto is a slice- edge result that aids in the fat- burni... | ["Optimum Max Keto Pills - Does It Have Side Effects?\nOPTIMAL MAX KETO REVIEWS \n\nOptimal Max Keto is a slice- edge result that aids in the fat- burning process. It allows you to lose weight naturally by releasing fat deposited in your body. This review will explain how it functions and how to use it. \n\nKeto supple... |
27,496 | Long-read DNA preparation for bacterial isolates. | 1 | dx.doi.org/10.17504/protocols.io.64ghgtw | https://www.protocols.io/view/long-read-dna-preparation-for-bacterial-isolates-64ghgtw | He Sun, Christian Brandt, Anna Schnürer | TITLE: Long-read DNA preparation for bacterial isolates.
AUTHORS: He Sun, Christian Brandt, Anna Schnürer
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">General:</span></div><div class = "text-block">This is an optimized DNA isolation protocol adapted to the proper... | ["[Samples preparation]\nAdd cation-adjusted Müller-Hinton broth in a 15 ml Falcon tube. (Müller-Hinton medium is used here for antibiotic-resistant bacteria)\n[CAMHB]", "[Samples preparation]\nAdd 1/4 10 µl-loop of colony from an agar plate in the solution.", "[Samples preparation]\nVortex the mixture for 5 s at maxim... |
40,996 | Nuclei Isolation for Single-Nuclei RNA sequencing | 4 | dx.doi.org/10.17504/protocols.io.bkacksaw | https://www.protocols.io/view/nuclei-isolation-for-single-nuclei-rna-sequencing-bkacksaw | Danh Truong, Salah-Eddine Lamhamedi-Cherradi, Joseph A. Ludwig | TITLE: Nuclei Isolation for Single-Nuclei RNA sequencing
AUTHORS: Danh Truong, Salah-Eddine Lamhamedi-Cherradi, Joseph A. Ludwig
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">We developed this protocol while trying to isolate single-nuclei from frozen liposarcoma tissue. We tested this protocol o... | ["[Nuclei Isolation (most tissues)]\nUse a scalpel and mince tissue on ice. Mincing tissue will improve nuclei isolation efficiency.", "[Nuclei Isolation (most tissues)]\nAddof EZ Lysis Buffer to tissue in a 1.5 mL microcentrifuge tube.\n500 µl", "[Nuclei Isolation (most tissues)]\nHomogenize tissue using plastic micro... |
94,883 | Parallel rapid expression and purification of proteins for crystallography (PREPX): 48x 100 mL cultures | 1 | dx.doi.org/10.17504/protocols.io.yxmvm35zbl3p/v2 | https://www.protocols.io/view/parallel-rapid-expression-and-purification-of-prot-c8wbzxan | michael.fairhead | TITLE: Parallel rapid expression and purification of proteins for crystallography (PREPX): 48x 100 mL cultures
AUTHORS: michael.fairhead
[DESCRIPTION]
This protocol details the parallel rapid expression and purification of 24x proteins for crystallography (PREPX) at 100 mL culture scale. Recombinant proteins are expre... | ["[Expression] Either transform BL21 (DE3) with the appropriate plasmid OR streak from glycerol stock onto 24 well agar plate and incubate 240 min 37 °C*.", "[Expression] Grow 1.5 mL 240 min in suitable container (2 mL 96-well plate, 15 ml faclon) of each clone in superbroth + 1 % glucose + the appropriate antibiotics... |
86,872 | Tri-plex staining for IBA-1, CD4, and CSF1R detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues | 4 | dx.doi.org/10.17504/protocols.io.bp2l6xx6zlqe/v1 | https://www.protocols.io/view/tri-plex-staining-for-iba-1-cd4-and-csf1r-detectio-cy3yxypw | Jayne E Wiarda, Crystal L Loving | TITLE: Tri-plex staining for IBA-1, CD4, and CSF1R detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues
AUTHORS: Jayne E Wiarda, Crystal L Loving
[DESCRIPTION]
A protocol for staining of protein (IBA-1) and RNA (CD4, CSF1R) in pig tissues
[BEFORE_START]
Starting specimens:
Starting samples = FFPE tissues... | ["[Baking] Before starting the assay: \nPreheat a dry oven to 60℃\nLoad slides for assay into vertical slide rack\n\nBaking\nBake slides 30 min 60℃\nOptional stopping point: store slides in a dry place & use within 1 week\n\nWhile slides bake:\nPrepare 0.05% PBS-T (can store at RT up to 1 month)\nPrepare 1X Co-Detectio... |
null | null | null | dx.doi.org/10.17504/protocols.io.eucbesw | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p><strong>STOCK SOLUTIONS:</strong><br /><br /> 1) 10.0 gm MgSO<sub>4</sub>·7H<sub>2</sub>O per liter d-H<sub>2</sub>O<br /><br /> 2) 1.0 gm KNO<sub>3</sub> per liter d-H<sub>2</sub>O<br /><br /> 3) 1.0 gm K<sub>2</sub>HPO<sub>4</sub> per liter d-H<sub>2</sub>O<br /><br /> 4) 50... | [] |
95,374 | CTAB | 4 | dx.doi.org/10.17504/protocols.io.rm7vzxz14gx1/v1 | https://www.protocols.io/view/ctab-c9dnz25e | George Odette | TITLE: CTAB
AUTHORS: George Odette
[DESCRIPTION]
Effective extraction and purification of DNA from mycobacteria is critical for downstream molecular applications. However, mycobacteria have a complex cell wall structure that makes DNA extraction challenging. Here, we describe an optimized protocol for extracting DNA ... | ["Grow strains of interest on Löwenstein-Jensen medium at 37°C until growth becomes clearly visible", "Transfer an appropriate number (1-3 inoculation loops) of bacterial cells into a microcentrifuge tube containing 400 μl TE-Puffer", "Incubate for 20 min at 80° C in a water bath to kill bacteria (check temperature wit... |
20,254 | Dissection and fixation of murine colonic tissue for myenteric plexus visualization | null | dx.doi.org/10.17504/protocols.io.xz6fp9e | null | Dante Heredia, Terence Smith | TITLE: Dissection and fixation of murine colonic tissue for myenteric plexus visualization
AUTHORS: Dante Heredia, Terence Smith
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for harvest of colonic intestinal tissue, with the intent of imaging the myenteric plexus.</div></div>
[STEPS]
?.... | ["A ventral midline incision is made and the whole colon is carefully excised into a Sylguard lined dissection dish.", "Cut along the mesenteric border until the colonic tube is now a rectanular in shape.", "Pin the colon at 110% of length and width mucosa side up. Gently remove the mucosal layer. Re-pin in a new Sylgu... |
36,294 | Analysis of clinical features and early warning signs in patients with severe COVID-19: a retrospectivecohort study | 1 | dx.doi.org/10.17504/protocols.io.bfpejmje | https://www.protocols.io/view/analysis-of-clinical-features-and-early-warning-si-bfpejmje | Xinpei Yue, Xinkui Liu | TITLE: Analysis of clinical features and early warning signs in patients with severe COVID-19: a retrospectivecohort study
AUTHORS: Xinpei Yue, Xinkui Liu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The Coronavirus disease 2019 (COVID-19) was first identified in Wuhan, China during December of ... | [] |
62,606 | Keto Advanced 1500 Review- Ingredients and Benefits, Price, Official Website -2022 | 3 | dx.doi.org/10.17504/protocols.io.261gen2kjg47/v1 | https://www.protocols.io/view/keto-advanced-1500-review-ingredients-and-benefits-b9dnr25e | bawi.jaice | TITLE: Keto Advanced 1500 Review- Ingredients and Benefits, Price, Official Website -2022
AUTHORS: bawi.jaice
[DESCRIPTION]
Different elements can cause weight gain, including an absence of satisfactory active work to consume calories. An individual ought to spend no less than 150 minutes out of every week on extrao... | [] |
27,336 | Vandy - Mouse Myocardial Infarction | null | dx.doi.org/10.17504/protocols.io.6xghfjw | null | Lin Zhong, Jeffrey Rottman, Chee Lim | TITLE: Vandy - Mouse Myocardial Infarction
AUTHORS: Lin Zhong, Jeffrey Rottman, Chee Lim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">The most common cause of cardiovascular mortality in man is the outcome from myoca... | ["Mice are anesthetized with pentobarbital (50 mg.kg, IP).", "The ventral neck and left parasternal region is shaved and disinfected with Betadine followed by 70% alcohol.", "The mouse is positioned supineon a heating pad and a small incision is made through the skin underlying the trachea.", "The trachea is exposed, a... |
35,310 | Ultrafiltration and purification of conditioned media (Pall Jumbsosep and Izon qEV-10) | 1 | dx.doi.org/10.17504/protocols.io.beqnjdve | https://www.protocols.io/view/ultrafiltration-and-purification-of-conditioned-me-beqnjdve | Joshua Welsh, Julia Kepley, Bryce Killingsworth, Tim Traynor, Jennifer Jones | TITLE: Ultrafiltration and purification of conditioned media (Pall Jumbsosep and Izon qEV-10)
AUTHORS: Joshua Welsh, Julia Kepley, Bryce Killingsworth, Tim Traynor, Jennifer Jones
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for ultrafiltration of cell culture conditioned media using P... | ["[Preparing the Pall Jumbosep device]\nMake up 70% ethanol with HPLC or other pure water", "[Preparing the Pall Jumbosep device]\nFilter at least 20 mL of the 70% ethanol with a 0.02 µm syringe-driven filter, or other small pore size filter", "[Preparing the Pall Jumbosep device]\nObtain a Jumbosep sample reservoir, a... |
105,748 | A simple and rapid protocol for real-time PCR detection of monkeypox virus (all clades including Ib) | 0 | dx.doi.org/10.17504/protocols.io.bp2l62yp1gqe/v1 | https://www.protocols.io/view/a-simple-and-rapid-protocol-for-real-time-pcr-dete-djhu4j6w | Sudhir Bhatia, Gudrun Baersch | TITLE: A simple and rapid protocol for real-time PCR detection of monkeypox virus (all clades including Ib)
AUTHORS: Sudhir Bhatia, Gudrun Baersch
[DESCRIPTION]
Monkeypox virus is re-emerging. It is causing outbreaks again in many countries, especially in African countries such as Congo, and poses a threat to other ... | ["Thaw one tube each: A, B, D1 and D2. If the kit is not in use, store them at -20°C. Keep tubes away from sunlight.", "Mark your microtubes with a sample number, positive and negative Control.", "Thaw tube A. Add 8µl of Tube A to each tube. Otherwise use a 96 microwell plates.", "Add 10µl of B to each microtube. Avoid... |
null | null | null | dx.doi.org/10.17504/protocols.io.rumd6u6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Zebrafish, and other small teleosts, are used as experimental models to evaluate human pathologies, including those linked to oxidative stress. The protocol presents an optimized technique to evaluate the activity of catalase, an important antioxidant enzyme, in zebrafish tis... | [] |
54,401 | in vitro assembly and transformation | 1 | null | https://www.protocols.io/view/in-vitro-assembly-and-transformation-bzc9p2z6 | Yuichiroh Ikagawa | TITLE: in vitro assembly and transformation
AUTHORS: Yuichiroh Ikagawa
[DESCRIPTION]
For the in vitro assembly of the DNA fragments, we decided to use the NEBuilder®, an assembly kit based on the Gibson Assembly. In NEBuilder®, exonucleases break down the ends of the DNA fragments and hybridize the cohesive ends, fol... | ["[in vitro assembly] DNA solutions and reagents were mixed according to the compositions in the table below.", "[in vitro assembly] Thaw the DNA solution and NEBuilder® Assembly Master Mix on ice.", "[in vitro assembly] Mix the DNA solution by vortexing, and centrifuge to collect the solution to the bottom of the tube... |
28,879 | Breeding Scheme and Selection of Animals for DiaComp Experiments | null | dx.doi.org/10.17504/protocols.io.8fphtmn | null | Nobuyo Maeda, Oliver Smithies, Nobuyuki Takahashi | TITLE: Breeding Scheme and Selection of Animals for DiaComp Experiments
AUTHORS: Nobuyo Maeda, Oliver Smithies, Nobuyuki Takahashi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary</span></div><div class = "text-block"><span>It is extremely important to measur... | ["When testing the effects of homozygosity for a mutant (such as a knockout)Testgene on diabetes induced by the dominant Ins2Akita mutation, a conservative and usually trouble-free breeding scheme is: Testgene mutant/wt & Ins2 wt/wt female (Inbred1) x Testgene mutant/wt & Ins2 Akita/wt male (Inbred2)Males and females o... |
null | null | null | dx.doi.org/10.17504/protocols.io.rund6ve | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Superoxide dismutase (SOD) activity is evaluated by means of a previously described spectrophotometric method [48]. </p>
[STEPS] | [] |
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