id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.ebibake | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
These are protocols from: <br /><br />Stedman, K. M., K. Porter, and M. L. Dyall-Smith. 2010. Chapter 6: The isolation of viruses infecting Archaea. Manual of Aquatic Viral Ecology. Waco, TX:American Society of Limnology and Oceanography. doi:10.4319/mave.2010.978-0-9845591-0-7<... | [] |
87,311 | Salivary collection participant instructions using OMNIgene ORAL OM 501 device | 1 | dx.doi.org/10.17504/protocols.io.n92ldm8qol5b/v1 | https://www.protocols.io/view/salivary-collection-participant-instructions-using-czhpx35n | Elisa Menozzi, Roxana Mezabrovschi, Sara Lucas, Jane Macnaughtan, Sofia Koletsi, Aleesa Nazeer, Nadine Loefflad | TITLE: Salivary collection participant instructions using OMNIgene ORAL OM 501 device
AUTHORS: Elisa Menozzi, Roxana Mezabrovschi, Sara Lucas, Jane Macnaughtan, Sofia Koletsi, Aleesa Nazeer, Nadine Loefflad
[DESCRIPTION]
This is an adapted protocol for collection of salivary samples from participants. This protocol wa... | [] |
97,583 | Nova-ST Chip Preparation Protocol | 0 | dx.doi.org/10.17504/protocols.io.n92ld835ov5b/v1 | https://www.protocols.io/view/nova-st-chip-preparation-protocol-dbip2kdn | Suresh Poovathingal, Kristofer Davie, Stein Aerts | TITLE: Nova-ST Chip Preparation Protocol
AUTHORS: Suresh Poovathingal, Kristofer Davie, Stein Aerts
[DESCRIPTION]
Nova-ST is a an open-source, high-resolution sequencing based spatial transcriptomics workflow. This method gives comparable resolution to BGI Stereoseq, SeqScope & PIXEL seq. Nova-ST is derived from dense... | ["[HDMI Sequencing] Before Starting", "[HDMI Sequencing] The NovaSeq S4 reagents stored at -20 °C is taken out and thawed at 4 °C for atleast 960 min -1200 min before sequencing. Prior to sequencing these reagents are removed and warmed to 25 °C for atleast 30 min .", "[HDMI Sequencing] The NovaSeq S4 flowcell stor... |
36,574 | Chloroform-Methanol Protein Extraction for Gram-negative Bacteria (High Throughput) | null | dx.doi.org/10.17504/protocols.io.bfx6jpre | https://www.protocols.io/view/chloroform-methanol-protein-extraction-for-gram-ne-bfx6jpre | Jennifer Gin, Yan Chen, Christopher Petzold | TITLE: Chloroform-Methanol Protein Extraction for Gram-negative Bacteria (High Throughput)
AUTHORS: Jennifer Gin, Yan Chen, Christopher Petzold
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Recent improvements in the speed and sensitivity of liquid chromatography-mass spectrometry systems have dri... | ["[Protein extraction]\nThaw cells at\non ice\nNote: If transferring directly from active cultures, omit this step. Adapt as needed for your specific organism and culturing conditions.", "[Protein extraction]\nTransfer 2-4 OD*mLs of cells to 8-Strip PCR tubes (Axygen, Cat.#14-222-251) or a 96-well PCR plate (ThermoFish... |
108,953 | Genomic Assembly of Plasmid DNA from Bacterial Cultures | 0 | null | https://www.protocols.io/view/genomic-assembly-of-plasmid-dna-from-bacterial-cul-dnmz5c76 | Ryan Teague | TITLE: Genomic Assembly of Plasmid DNA from Bacterial Cultures
AUTHORS: Ryan Teague
[DESCRIPTION]
This protocol was developed for BIT 495: Special Topics in Biotechnology: Portable Genome Sequencing at North Carolina State University.
The purpose of this protocol is to consistently complete genomic assem... | ["[1. Plasmid Extraction from Bacterial Colony] To start, obtain an overnight culture of bacteria (of whatever type of bacteria)", "[1. Plasmid Extraction from Bacterial Colony] Centrifuge the culture to pellet the bacteria", "[1. Plasmid Extraction from Bacterial Colony] Remove all supernatant and resuspend the bacter... |
49,527 | Whole-genome sequencing of two human rhinovirus A types - A15 and A101 | 4 | dx.doi.org/10.17504/protocols.io.bukxnuxn | https://www.protocols.io/view/whole-genome-sequencing-of-two-human-rhinovirus-a-bukxnuxn | Martha Luka, Everlyn Kamau, Zaydah R. de Laurent, John Mwita Morobe, Leonard K. Alii, Charles Agoti, D. James Nokes | TITLE: Whole-genome sequencing of two human rhinovirus A types - A15 and A101
AUTHORS: Martha Luka, Everlyn Kamau, Zaydah R. de Laurent, John Mwita Morobe, Leonard K. Alii, Charles Agoti, D. James Nokes
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align... | ["[RNA extraction]\nExtract viral RNA from 140 μl of sample using the QIAamp viral RNA mini kit according to the manufacturer's recommendations, eluting in 60 μl of EB buffer", "[Reverse transcription]\nCombine the following in a 1.5 ml tube:ComponentVolumeRNA5 μlDEPC-treated water3 μlrandom primer (10 μM)1 μl10 mM dNT... |
null | null | null | dx.doi.org/10.17504/protocols.io.dnn5dd | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
The NEXTflex™ mtDNA-Seq Kit is designed to prepare single or paired-end DNA libraries of mitochondrial DNA (mtDNA) for sequencing using Illumina® platforms. The procedure isolates mitochondrial DNA from genomic DNA (gDNA) by selective digestion of linear nuclear DNA, followed by... | [] |
102,398 | Maintenance & Differentiation: SHSY-5Y Neuroblastoma Cells | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj6r2lx1/v1 | https://www.protocols.io/view/maintenance-amp-differentiation-shsy-5y-neuroblast-df863rze | Md Razaul Karim | TITLE: Maintenance & Differentiation: SHSY-5Y Neuroblastoma Cells
AUTHORS: Md Razaul Karim
[DESCRIPTION]
This protocol details the maintenance & differentiation of SHSY-5Y Neuroblastoma Cells.
[STEPS]
SECTION: Maintenance
1. For regular maintenance of SHSY-5Y cells, use DMEM full media.
SECTION: Differentiation (... | ["[Maintenance] For regular maintenance of SHSY-5Y cells, use DMEM full media.", "[Differentiation (SHSY-5Y) Cells: About 5-7 days differentiation. No need Glutamax] Day-01 (Mon): Plating with DMEM full media \n\nWarm DMEM full media, PBS, and Trypsin in the 37 °C bead bath for 30 min. Clean the working area by using 7... |
81,834 | Multiple Targets Identified via Tagmentation (MulTI-Tag) v1.0 | 4 | null | https://www.protocols.io/view/multiple-targets-identified-via-tagmentation-multi-ct6iwrce | Michael Meers Lab | TITLE: Multiple Targets Identified via Tagmentation (MulTI-Tag) v1.0
AUTHORS: Michael Meers Lab
[DESCRIPTION]
We introduce a public protocol for Multiple Targets Identified via Tagmentation (MulTI-Tag), a chromatin profiling approach that acertains the genomic enrichment of multiple chromatin protein targets in the sa... | ["[Conjugate generation (4 hours)] Resuspend dehydrated, 5’-aminated (NH2) P5_i5 oligo in 1xPBS at a concentration of 200 µM.", "[Conjugate generation (4 hours)] Resuspend dehydrated Tn5MErev oligo (5'-[phos]CTGTCTCTTATACACATCT-3') in 1xPBS at a concentration of 200 µM.", "[Conjugate generation (4 hours)] Anneal P5_i5 ... |
45,539 | HuBMAP | Sectioning of FFPE Specimens | 4 | dx.doi.org/10.17504/protocols.io.bqqbmvsn | https://www.protocols.io/view/hubmap-sectioning-of-ffpe-specimens-bqqbmvsn | Kelley Knizner, Christopher Simmons | TITLE: HuBMAP | Sectioning of FFPE Specimens
AUTHORS: Kelley Knizner, Christopher Simmons
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This method details sectioning of the FFPE HuBMAP specimens.</div><div class = "text-block"><span>Protocol adopted from </span><span style = "font-style:itali... | ["[PREPARATION OF MICROTOME]\nFill flotation bath with de-ionized or purified water. Set the water temperature to .\n42 °C", "[PREPARATION OF MICROTOME]\nInspect microtome to ensure it is clean, well-maintained, and set at the right angle.", "[PREPARATION OF TISSUE BLOCKS ]\nEnsure the tissue blocks are correctly embed... |
null | null | null | dx.doi.org/10.17504/protocols.io.gupbwvn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is a guide to using IRDye Subclass Specific antibodies for Western blotting. For more detailed descriptions of Western blotting, refer to Western Blot Analysis and In-Cell Western Kits I and II on the LI-COR Biosciences website (<a href="http://www.licor.com" ta... | ["{\"blocks\":[{\"key\":\"akuqo\",\"text\":\"Wet the membrane in PBS for several minutes.\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"1fajm\",\"text\":\" \",\"type\":\"atomic\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[{\"offset\":0,\"length\":1,... |
28,897 | Fluorescence measurement | null | dx.doi.org/10.17504/protocols.io.8f9htr6 | null | iGEM Dusseldorf | TITLE: Fluorescence measurement
AUTHORS: iGEM Dusseldorf
[STEPS]
?. Prepare 5 ml LB medium with antibiotic resistance and 0.5% ml Tergitol
?. Inoculate one colony in the prepared LB medium
?. Add 0.01-1 mM of the desired fatty acid to the culture medium
?. Inoculate the culture for at least 16 hours at 37 °C
?. Measur... | ["Prepare 5 ml LB medium with antibiotic resistance and 0.5% ml Tergitol", "Inoculate one colony in the prepared LB medium", "Add 0.01-1 mM of the desired fatty acid to the culture medium", "Inoculate the culture for at least 16 hours at 37 °C", "Measure the absorption with a photometer at a wavelength 600 nm", "Dilute... |
28,041 | Nanoluciferase Assay in C. elegans | null | dx.doi.org/10.17504/protocols.io.7mhhk36 | null | Emily Troemel, Ivana Sfarcic, Theresa B, Erin C Daniels | TITLE: Nanoluciferase Assay in C. elegans
AUTHORS: Emily Troemel, Ivana Sfarcic, Theresa B, Erin C Daniels
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>A highly sensitive Nanoluciferase (NanoLuc)-based method in multi-well format to detect constitutive and inducible gene expression in whole... | ["A.Grow up/Treat Worms and Prepare Lysate\n\nGrow up and/or treat worms as required, usually on 3.5 or 6 cm NGM plates seeded with OP50-1. N2 can be included as no-Nanoluciferase control, ERT513 (unc-119 (ed3) III; jySi35 [pET618 (vha-6p::NANOLUC::unc-54 3' UTR, unc-119 (+)])or ERT529 (unc-119 (ed3) III; jySi40 [pET63... |
62,200 | Mapping dichotomising colon and bladder sensory afferent neurons and terminals and if they undergo structural plasticity post-colitis. | 1 | dx.doi.org/10.17504/protocols.io.j8nlkk971l5r/v2 | https://www.protocols.io/view/mapping-dichotomising-colon-and-bladder-sensory-af-b8yyrxxw | Andrea Harrington | TITLE: Mapping dichotomising colon and bladder sensory afferent neurons and terminals and if they undergo structural plasticity post-colitis.
AUTHORS: Andrea Harrington
[DESCRIPTION]
This protocol is used to identify the sensory afferent neurons innervating the colon and bladder in dorsal root ganglia and nodose gan... | ["Perfuse fixation\nFour days after retrograde tracing surgery, to identify CTB labelled afferent cell bodies and seven days after retrograde tracing surgery to identify CTB labelled terminals mice underwent transcardial perfuse fixation as per Harrington AM. et al.. Colonic afferent input and dorsal horn neuron activa... |
38,771 | Phenzine Oxidizer Enrichment and Isolation | 4 | dx.doi.org/10.17504/protocols.io.bh4tj8wn | https://www.protocols.io/view/phenzine-oxidizer-enrichment-and-isolation-bh4tj8wn | Lev Tsypin, Yinon Bar-On, Scott Saunders, Jared R Leadbetter, Dianne K Newman | TITLE: Phenzine Oxidizer Enrichment and Isolation
AUTHORS: Lev Tsypin, Yinon Bar-On, Scott Saunders, Jared R Leadbetter, Dianne K Newman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol for the enrichment of phenazine-1-carboxylic acid oxidizing microbes from a soil sample.</div><... | ["[Medium compostion]\nStock solutions100x Freshwater salts:1.71 M sodium chloride197 mM magnesium chloride68 mM calcium chloride671 mM potassium chloride1000x Trace Elements:20 mM HCl7.5 mM FeCl3 6H2O480 uM H3BO3500 uM MnCl2 4H2O6.8 mM CoCl2 6H2O1 mM NiCl2 6H2O12 uM CuCl2 2H2O500 uM ZnCl223 uM Na2SeO3150 uM Na2MoO4100... |
95,298 | Stable Transfection of Plasmid DNA into Adherent Rodent Cell Lines Using Calcium Phosphate | 1 | dx.doi.org/10.17504/protocols.io.rm7vzxz5rgx1/v1 | https://www.protocols.io/view/stable-transfection-of-plasmid-dna-into-adherent-r-c9baz2ie | Vipasha Dwivedi, Jen Davis, Ella Wilson, Markelle Scott, Madison Zelan, Nicholas DeFeo, Victoria Huffman, Rees Cooke, Kaitlyn O'Daniel, David J Riese II | TITLE: Stable Transfection of Plasmid DNA into Adherent Rodent Cell Lines Using Calcium Phosphate
AUTHORS: Vipasha Dwivedi, Jen Davis, Ella Wilson, Markelle Scott, Madison Zelan, Nicholas DeFeo, Victoria Huffman, Rees Cooke, Kaitlyn O'Daniel, David J Riese II
[DESCRIPTION]
A traditional strategy for stably transfectin... | ["[Introduction] A traditional strategy for stably transfecting DNA into rodent fibroblast cell lines features calcium phosphate precipitates. Here we describe our laboratory protocol for this strategy. We have assumed that the transfected DNA contains an expression vector for an antibiotic resistance gene, such as t... |
86,047 | Mouse Lung Inflation Protocol for Mass Spectrometry Imaging (DMS22) | 1 | null | https://www.protocols.io/view/mouse-lung-inflation-protocol-for-mass-spectrometr-cx97xr9n | Alison J Scott, rkernst | TITLE: Mouse Lung Inflation Protocol for Mass Spectrometry Imaging (DMS22)
AUTHORS: Alison J Scott, rkernst
[DESCRIPTION]
This protocol describes the preparation of inflated mouse lungs with gelatin for use in subsequent MSI analyses.
[BEFORE_START]
MATERIALS:
Porcine gelatin, ~300g Bloom, Type A (Sigma #G1890)
Mol... | ["[PROTOCOL:] Euthanize mice according to IACUC approved protocols. Pinch the foot firmly using a thumb nail to ensure the mouse is dead and non-responsive.", "[PROTOCOL:] Lay the animal on its back and pin its arms and legs to the Styrofoam board in an X-like shape. Pin the head back with one pin in the fore snout so ... |
70,258 | Discovery proteomic (DIA) LC-MS/MS data acquisition and analysis | 1 | dx.doi.org/10.17504/protocols.io.e6nvwk1z7vmk/v2 | https://www.protocols.io/view/discovery-proteomic-dia-lc-ms-ms-data-acquisition-cgustwwe | Yan Chen, Jennifer Gin, Christopher J Petzold | TITLE: Discovery proteomic (DIA) LC-MS/MS data acquisition and analysis
AUTHORS: Yan Chen, Jennifer Gin, Christopher J Petzold
[DESCRIPTION]
This protocol details steps in discovery proteomic data-independent acquisition with a standard-flow UHPLC-Obitrap system and a subsequent DIA-NN library-free database search. Th... | ["[Proteomics: HPLC and Mass Spectromtery] Thaw peptide samples on ice , and transfer 30 µLof each sample to LC autosampler vials (Agilent, Cat.#5182-0567,#5182-0564) or 96-well plate (Bio-Rad, Cat.#HSP9655).", "[Proteomics: HPLC and Mass Spectromtery] Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis... |
80,279 | Rapid Ribosome (Polysome) Profiling | 4 | dx.doi.org/10.17504/protocols.io.q26g7ykmqgwz/v1 | https://www.protocols.io/view/rapid-ribosome-polysome-profiling-csmxwc7n | Jason D Limberis, Yuxiang Chen | TITLE: Rapid Ribosome (Polysome) Profiling
AUTHORS: Jason D Limberis, Yuxiang Chen
[DESCRIPTION]
Ribosome profiling is a powerful technique used to study translation at a genome-wide level. It involves the sequencing of ribosome-protected mRNA fragments to determine the positions of ribosomes on transcripts. This info... | ["[Linker ligation] Add 1 µL of 100 micromolar (µM) RiboS_linker (linker RNA oligo) to 10 µL of RNA, denature it for1 min at 70 °C, and then cool it to Room temperature", "[Linker ligation] Set up the ligation reaction below and incubate for 90 min at 37 °C", "[RT (TSO) reaction] Mix thoroughly by gently pipetting up a... |
25,208 | RNA Isolation from Plant Tissue Protocol 16: CTAB-Hot Acid Phenol Method for Algae | null | dx.doi.org/10.17504/protocols.io.4uygwxw | null | null | TITLE: RNA Isolation from Plant Tissue Protocol 16: CTAB-Hot Acid Phenol Method for Algae
AUTHORS:
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Implemented by: Falicia Goh and Neil Clarke</div><div class = "text-block"><span>This RNA isolation method is a combination and modification of the hot ... | ["Preheat phenol and phenol:chloroform to .\n65 °C\nHeated phenol should not be re-used.", "Collect algae cells via centrifugation for at at .\n0 Room temperature", "Re-suspend the frozen pellet in of preheated extraction buffer.\n800 µl", "Incubate at for . Gently vortex every .\n65 °C", "Cool to .\n0 Room tempera... |
69,418 | B. burgdorferi enrichment from feeding ticks | 4 | null | https://www.protocols.io/view/b-burgdorferi-enrichment-from-feeding-ticks-cf2itqce | Anne Sapiro, Jenny Zhang, Beth Hayes, Seemay Chou | TITLE: B. burgdorferi enrichment from feeding ticks
AUTHORS: Anne Sapiro, Jenny Zhang, Beth Hayes, Seemay Chou
[DESCRIPTION]
Borrelia burgdorferi (Bb), the causative agent of Lyme disease, must adapt to vastly different environments as the bacterium cycles between the tick vector and a vertebrate host. During a blood... | ["[Homogenize infected ticks] Add ticks and 500 μL of PBS to the dounce grinder and homogenize using pestle A (large clearance pestle) 10 times up and down.", "[Homogenize infected ticks] Collect ticks for homogenization. We recommend using between 6-14 nymphal ticks depending on day of feeding. Ticks can be washed wit... |
29,106 | Dental emergency: scoping review protocol | null | dx.doi.org/10.17504/protocols.io.8nshvee | null | Karla Frichembruder, Camila Mello dos Santos, Fernando Neves Hugo | TITLE: Dental emergency: scoping review protocol
AUTHORS: Karla Frichembruder, Camila Mello dos Santos, Fernando Neves Hugo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;">Introduction: </span><span>considering the hi... | [] |
30,890 | Probe-Seq | null | dx.doi.org/10.17504/protocols.io.baeiibce | null | Ryoji Amamoto, Constance L. Cepko | TITLE: Probe-Seq
AUTHORS: Ryoji Amamoto, Constance L. Cepko
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Recent transcriptional profiling technologies are uncovering previously-undefined cell populations and molecular markers at an unprecedented pace. While single cell RNA (scRNA) sequencin... | ["Prepare all solutions.", "Dissect retinas in HBSS at RT.", "Place retina in microcentrifuge tube and remove most of the HBSS.", "Add 400 µL of the pre-warmed Papain Mix.", "Incubate for 7 minutes at 37oC with no agitation.", "Spin 600xg for 2.5 minutes at RT.", "Remove supernatant and add 1 mL of HBSS/FBS without agi... |
91,641 | test_protocol | 6 | null | https://www.protocols.io/view/test-protocol-c5qzy5x6 | isis.scott | TITLE: test_protocol
AUTHORS: isis.scott
[DESCRIPTION]
abstract
[STEPS]
SECTION: Scope
1. write about the protocol and the reason to run this experiment
SECTION: Safety
2. Safety googles
SECTION: Safety
3. Lab coat
SECTION: Safety
3.1. fire-resistant
SECTION: Procedures
4. Add 10 mL of 1 1 Molarity (m) NaCl solut... | ["[Scope] write about the protocol and the reason to run this experiment", "[Safety] Safety googles", "[Safety] Lab coat", "[Safety] fire-resistant", "[Procedures] Add 10 mL of 1 1 Molarity (m) NaCl solution to soil at25 °C in the oven"] |
null | null | null | dx.doi.org/10.17504/protocols.io.hfwb3pe | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
43,330 | one-step growth curve | 1 | dx.doi.org/10.17504/protocols.io.bnjamcie | https://www.protocols.io/view/one-step-growth-curve-bnjamcie | Jiaxin Li | TITLE: one-step growth curve
AUTHORS: Jiaxin Li
[STEPS]
?. Subculture host bacterium in medium of choice plus 2 mM CaCl2 and grow to mid-log phase (ca. 0.5 OD650nm).
?. Pipette 9.9 mL of the log phage culture into the empty flask and place at the appropriate incubation temperature for 5 min.
?. Add 0.1 mL of phage pre... | ["Subculture host bacterium in medium of choice plus 2 mM CaCl2 and grow to mid-log phase (ca. 0.5 OD650nm).", "Pipette 9.9 mL of the log phage culture into the empty flask and place at the appropriate incubation temperature for 5 min.", "Add 0.1 mL of phage preparation to the 9.9 mL culture. (FLASK A)", "Transfer 1.0 ... |
19,036 | Basic immunofluorescence protocol for adherent cells | null | dx.doi.org/10.17504/protocols.io.wt4feqw | null | Girija Goyal | TITLE: Basic immunofluorescence protocol for adherent cells
AUTHORS: Girija Goyal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Adherent cells were cultured in flat bottom plates and fixed in situ. This is a protocol to detect specific proteins by immunofluorescence in these cells. Immunofluorosce... | ["[Fixation]\nFix the cells using 4% paraformaldehyde (PFA) by removing media and submerging cells in PFA for at . Cells can be left in fixative for a few days at\n[room temp]\n[Fridge]", "[Permeabilization]\nFor the antibody to be able to penetrate the cells, the membranes need to be permeabilized. This may be necess... |
28,636 | Preparation of frozen nuclei for single-nucleus RNA seq | null | dx.doi.org/10.17504/protocols.io.774hrqw | null | Kimberly Siletti, Sten Linnarsson | TITLE: Preparation of frozen nuclei for single-nucleus RNA seq
AUTHORS: Kimberly Siletti, Sten Linnarsson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes our preparation of nuclei for single-nucleus RNA sequencing on the 10X Genomics Chromium system, starting from FAC-sorted ... | [] |
55,230 | Antioxidant rescue of C. elegans behaviour on Keio E. coli mutants | 1 | dx.doi.org/10.17504/protocols.io.bz66p9he | https://www.protocols.io/view/antioxidant-rescue-of-c-elegans-behaviour-on-keio-bz66p9he | Saul Moore | TITLE: Antioxidant rescue of C. elegans behaviour on Keio E. coli mutants
AUTHORS: Saul Moore
[DESCRIPTION]
Protocol for screening candidate behaviour-modifying E. coli BW25113 single-gene deletion mutants from the 'Keio Collection', to investigate their effects on Caenorhabditis elegans behaviour in the presence o... | ["[Preparing NGM agar + pouring plates] Prior to screening, prepare the materials needed for screening C. elegans on selected Keio E. coli mutants:\nFor a single experiment replicate (10 biological replicates of each mutant, screened in 2 runs with the laboratory's 'Hydra' imaging rig):\n\n- 12 Whatman 96-square-well f... |
75,428 | DNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Sanger Sequence Data | 1 | null | https://www.protocols.io/view/dna-barcoding-standard-operating-protocol-plants-a-cmwcu7aw | Laura L Forrest, David Bell, Michelle Hart | TITLE: DNA Barcoding Standard Operating Protocol, Plants and Lichens at RBGE, Sanger Sequence Data
AUTHORS: Laura L Forrest, David Bell, Michelle Hart
[DESCRIPTION]
This is part of the collection DToL Taxon-specific Standard Operating Procedures for the Plant Working Group (protocols.io). The SOP collection contains g... | ["[Trace file names] Before submitting samples for sequencing, generate trace file names that are in a standardized format as this will facilitate downstream processing.", "[Sequence editing] Assemble the forward and reverse traces, trim off low quality areas and primer sequences, and resolve any disagreements between ... |
64,794 | Isolation of ECs from Brain tissue for scRNAseq on the 10x Chromium | 4 | null | https://www.protocols.io/view/isolation-of-ecs-from-brain-tissue-for-scrnaseq-on-cbh2sj8e | Guido Krähenbühl | TITLE: Isolation of ECs from Brain tissue for scRNAseq on the 10x Chromium
AUTHORS: Guido Krähenbühl
[DESCRIPTION]
Protocols for endothelial cell isolation from mouse tissues: brain, addapted from
Open Access • DOI: 10.1016/j.xpro.2021.100508
[STEPS]
SECTION: Preparation
1. Day before experiment check Antibodies,... | ["[Preparation] Day before experiment check Antibodies, Enzyme Mix 1 and 2 Reagents to be used and prepare the following Buffer:", "[Preparation] Before starting prepare:\nDissection Tools\n20G needle and thin syringe\nIf Brain tissue is also taken, prepare perfusion kit\n10mL DMEM Genta\n\n \n\n\nKeep all reagents on ... |
21,629 | Parental Acceptance and children’s psychological adjustment | null | dx.doi.org/10.17504/protocols.io.zc5f2y6 | null | M. Angel Carrasco, Begoña Delgado, Fco. Pablo Holgado-Tello | TITLE: Parental Acceptance and children’s psychological adjustment
AUTHORS: M. Angel Carrasco, Begoña Delgado, Fco. Pablo Holgado-Tello
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><span>The protocols used in the paper titled “</span><span style = ... | [] |
99,579 | Establishment and Maintenance of Organotypic Cerebellar Slice Cultures (OCerSC) from Aged Mice | 0 | dx.doi.org/10.17504/protocols.io.q26g71428gwz/v1 | https://www.protocols.io/view/establishment-and-maintenance-of-organotypic-cereb-ddg323yn | Kaitlan S. Smith, Michael Fernandes de Almeida, Jonathan C Schisler | TITLE: Establishment and Maintenance of Organotypic Cerebellar Slice Cultures (OCerSC) from Aged Mice
AUTHORS: Kaitlan S. Smith, Michael Fernandes de Almeida, Jonathan C Schisler
[DESCRIPTION]
This protocol outlines a method for culturing organotypic slices from aged mouse cerebellar tissue, maintaining the cerebellum... | ["[Equipment]", "[Reagents]", "[Materials] Slice Culture Consumables:", "[Solutions] Slice Culture Media #1 \nNeurobasal-A Medium supplemented with:\n2% B27\n1% N2 Supplement\n1% Glutamax\n200 g/L glucose\n1.5% Penicillin/streptomycin\n1.5% Amphotericin B\n80 μM Indomethacin\n\nSlice Culture Media #2 \nNeurobasal-A Med... |
91,043 | F5 Targeted profiling method for metabolomics | 1 | null | https://www.protocols.io/view/f5-targeted-profiling-method-for-metabolomics-c46byzan | Megan Danielewicz | TITLE: F5 Targeted profiling method for metabolomics
AUTHORS: Megan Danielewicz
[DESCRIPTION]
This protocol details the preparation of reagents and samples and extraction of plasma.
[STEPS]
SECTION: Extraction protocol
3. Add sample into 1.5 mL eppendorf tube:
SECTION: Extraction protocol
4. Add 1000 µL of methanol (... | ["[Extraction protocol] Add sample into 1.5 mL eppendorf tube:", "[Extraction protocol] Add 1000 µL of methanol (preferably ice cold)", "[Extraction protocol] Vortex the sample/ solvent mixture for 10 s.", "[Extraction protocol] Centrifuge the sample/ solvent mixture at 10000 rcf, 10 min, 8 °C.", "[Extraction protocol]... |
97,399 | Assessment of implant accuracy using high-resolution postmortem MRI | 0 | dx.doi.org/10.17504/protocols.io.14egn6x86l5d/v1 | https://www.protocols.io/view/assessment-of-implant-accuracy-using-high-resoluti-dbcx2ixn | Lucy Liang, Elvira Pirondini, Jonathan C Ho | TITLE: Assessment of implant accuracy using high-resolution postmortem MRI
AUTHORS: Lucy Liang, Elvira Pirondini, Jonathan C Ho
[DESCRIPTION]
Deep brain implant accuracy is important for successful experiments in non-human primates. In this protocol, we describe the steps to use postmortem imaging to assess the accura... | ["[Thermal ablation & tissue preparation] Before euthanizing the animal according to protocol, perform a thermal lesion through the deep brain electrode. \n\nWe use a radiofrequency cannula (S-100 5 mm ActiveTip Straight cannula 22G, Abbott) and a radiofrequency electrode (RF-SE-10 Reusable Stainless Steel Electrod... |
70,019 | Dissection and immunohistochemistry of mouse brainstem | 4 | dx.doi.org/10.17504/protocols.io.4r3l275y3g1y/v1 | https://www.protocols.io/view/dissection-and-immunohistochemistry-of-mouse-brain-cgmbtu2n | Seol-Hee Kim, Thomas Taylor-Clark | TITLE: Dissection and immunohistochemistry of mouse brainstem
AUTHORS: Seol-Hee Kim, Thomas Taylor-Clark
[DESCRIPTION]
Mice are euthanized, perfused with fixative for brainstem tissue collection. Mouse brainstem is cryosectioned and slices are stained for protein expression using immunohistochemistry. Expression of sp... | ["[Tissue collection and cryosection] Mice are euthanized by CO2inhalation and transcardially perfused with ice-cold PBS followed by perfusion fixation with ice-cold 3.7% formaldehyde (FA).", "[Tissue collection and cryosection] Brainstem is dissected out and post-fixed for 4 h in 3.7% FA at 4°C. The brainstem is washe... |
61,929 | Peptide N-Terminal Modification | 6 | dx.doi.org/10.17504/protocols.io.8epv593bng1b/v1 | https://www.protocols.io/view/peptide-n-terminal-modification-b8qhrvt6 | Cathy Miller | TITLE: Peptide N-Terminal Modification
AUTHORS: Cathy Miller
[DESCRIPTION]
Although many of the most widely recognized post-translational modifications are characteristic of secretory or cell-surface proteins, most proteins, whatever their ultimate cellular desination, undergo some modification. For proteins synthesi... | [] |
23,038 | PreTect SEE | 1 | dx.doi.org/10.17504/protocols.io.2q6gdze | https://www.protocols.io/view/pretect-see-2q6gdze | Bente Marie Falang | TITLE: PreTect SEE
AUTHORS: Bente Marie Falang
[DESCRIPTION]
PreTect SEE is a CE-IVD kit for the qualitative detection of E6/E7 mRNA from HPV 16, 18 and 45 in a single analysis, identifying women at risk of having or developing cervical pre-cancer or cancer related to the carcinogenic HPV types included.
The kit is b... | ["Sample preparation and lysis of material:\nMix each sample thoroughly and pipette 1ml (PreservCyt) to a 10 ml sterile centrifuge tube. \nCentrifuge cells for 12 minutes at 2500 rpm (1125g)\nDiscard the supernatant with a Pasteur-pipette or by vacuum suction\nAdd 1 ml lysis buffer (PreTect X) to the cells and homogeni... |
104,392 | Baseplate Implantation for Two-Part Chamber System | 0 | dx.doi.org/10.17504/protocols.io.kqdg32b91v25/v1 | https://www.protocols.io/view/baseplate-implantation-for-two-part-chamber-system-dh7g39jw | Jiwon Choi, Usamma Amjad, Helen N Schwerdt | TITLE: Baseplate Implantation for Two-Part Chamber System
AUTHORS: Jiwon Choi, Usamma Amjad, Helen N Schwerdt
[DESCRIPTION]
This protocol describes how to implant a "baseplate" onto the skull. The baseplate forms the bottom layer of the full chamber, which is completed in the second surgical procedure by mounting a "... | ["[Baseplate Implantation (First part of the cranial chamber implant)] Procedures were performed on Rhesus monkeys (n = 2) and were approved by the Institute’s Animal Care and Use Committee (IACUC) at the University of Pittsburgh and were performed following the Guide for the Care and Use of Laboratory Animals (Departm... |
null | null | null | dx.doi.org/10.17504/protocols.io.kcgcstw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?. | [] |
36,615 | Intramuscular Injection Adult Mouse | null | dx.doi.org/10.17504/protocols.io.bfzfjp3n | https://www.protocols.io/view/intramuscular-injection-adult-mouse-bfzfjp3n | Allen Institute for Brain Science | TITLE: Intramuscular Injection Adult Mouse
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the general procedures used for intramuscular injection in adult mice. </div><div class = "text-block"><span style = "font-weight:bold;">Note... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.iaqcadw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
89,577 | Rabies Virus Bat-Clade Sequencing | 4 | dx.doi.org/10.17504/protocols.io.8epv5x3bng1b/v1 | https://www.protocols.io/view/rabies-virus-bat-clade-sequencing-c3qhymt6 | Fernanda Godinho, Aline Campos, rosana-huff, Thales Bermann, Amanda Ruivo, Milena Bauermann, Gabriel Luz Wallau, Richard Salvato | TITLE: Rabies Virus Bat-Clade Sequencing
AUTHORS: Fernanda Godinho, Aline Campos, rosana-huff, Thales Bermann, Amanda Ruivo, Milena Bauermann, Gabriel Luz Wallau, Richard Salvato
[DESCRIPTION]
Join us in advancing global genomic surveillance of the rabies virus.
We are actively engaged in pioneering a pan-clade whole... | ["[Primer Preparation] Reconstitute each primer shown in Table 1 (See Materials section), using nuclease-free water to get a 100 µM stock solution.", "[Primer Preparation] Prepare RABV-BAT primer pools A and B as described here.", "[Primer Preparation] Separate all primers at 100 µM into two separate boxes labeled as P... |
31,399 | Effects of Vagus Nerve Stimulation/Gastric Electrical Stimulation on Gastric Emptying and Motility Assessed with Magnetic Resonance Imaging | 1 | dx.doi.org/10.17504/protocols.io.bawfifbn | https://www.protocols.io/view/effects-of-vagus-nerve-stimulation-gastric-electri-bawfifbn | Kun-Han Lu, Zhongming Liu, Jiayue Cao | TITLE: Effects of Vagus Nerve Stimulation/Gastric Electrical Stimulation on Gastric Emptying and Motility Assessed with Magnetic Resonance Imaging
AUTHORS: Kun-Han Lu, Zhongming Liu, Jiayue Cao
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the methods used to evaluate the e... | ["[Acute effects of vagus nerve stimulation settings on gastric motility assessed with MRI]\nStudy titleAcute effects of vagus nerve stimulation settings on gastric motility assessed with MRIDescriptionThis protocol describes the methods used to evaluate the effects of VNS settings on gastric motility in rats. Briefly,... |
25,835 | Calibration Protocol - Conversion of OD600 to Colony Forming Units (CFUs) | null | dx.doi.org/10.17504/protocols.io.5gjg3un | null | Paul Rutten, Richard Tennant, Jacob Beal, Christopher Workman, Traci Haddock-Angelli, Natalie Farny, Vinoo Selvarajah | TITLE: Calibration Protocol - Conversion of OD600 to Colony Forming Units (CFUs)
AUTHORS: Paul Rutten, Richard Tennant, Jacob Beal, Christopher Workman, Traci Haddock-Angelli, Natalie Farny, Vinoo Selvarajah
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This procedure can be used to calibrat... | ["[Sample Preparation]\nThis protocol will result in CFU/mL for 0.1 OD600. Your overnight cultures will have a much higher OD600 and so this section of the protocol, called “Sample Preparation”, will give you the “Starting Sample” with a 0.1 OD600 measurement.", "[Sample Preparation]\nMeasure the OD600 of your cell cul... |
98,589 | Self-made chrome alum gelatin coated slides | 0 | dx.doi.org/10.17504/protocols.io.3byl49kkogo5/v1 | https://www.protocols.io/view/self-made-chrome-alum-gelatin-coated-slides-dch52t86 | Sonja Fritzsche | TITLE: Self-made chrome alum gelatin coated slides
AUTHORS: Sonja Fritzsche
[DESCRIPTION]
This is step-wise protocol to coating microscopy slides with chrom alum gelatin for better tissue adherence.
[STEPS]
SECTION: Prepare chrome alum gelatin solution
1. Heat up 200 mL ddH2O to 50 °C on hot plate using a magnetic st... | ["[Prepare chrome alum gelatin solution] Heat up 200 mL ddH2O to 50 °C on hot plate using a magnetic stirrer.", "[Prepare chrome alum gelatin solution] Add 1 g gelatin gradually until completely dissolved to prevent formation of clumps. Solution has to be clear.", "[Prepare chrome alum gelatin solution] Let solution co... |
61,107 | Isolation of Nuclei from Adult_Human_Brain_Tissue | 1 | dx.doi.org/10.17504/protocols.io.ewov149p7vr2/v2 | https://www.protocols.io/view/isolation-of-nuclei-from-adult-human-brain-tissue-b7wtrpen | Allen Institute for Brain Science | TITLE: Isolation of Nuclei from Adult_Human_Brain_Tissue
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for FPCR and/or RNA-seq analysis.
Note: Research reported in this publication was supported by... | [] |
103,090 | Purification of BCL2L13-GFP | 0 | dx.doi.org/10.17504/protocols.io.36wgqno2ogk5/v1 | https://www.protocols.io/view/purification-of-bcl2l13-gfp-dgws3xee | Elias Adriaenssens | TITLE: Purification of BCL2L13-GFP
AUTHORS: Elias Adriaenssens
[DESCRIPTION]
This protocol details the purification of BCL2L13-GFP.
[STEPS]
SECTION: Purification - BCL2L13-GFP
1. To purify GFP-tagged
BCL2L13-GFP (available from Addgene),
BCL2L13(W276A/I279A)-GFP (ΔLIR1) (available from Addgene),
BCL2L13(Y213A/I21... | ["[Purification - BCL2L13-GFP] To purify GFP-tagged \n\nBCL2L13-GFP (available from Addgene), \nBCL2L13(W276A/I279A)-GFP (ΔLIR1) (available from Addgene), \nBCL2L13(Y213A/I216A/W276A/I279A)-GFP (ΔLIR1+2) (available from Addgene),\nBCL2L13(I224A/L227A/W276A/I279A)-GFP (ΔLIR1+3)(available from Addgene),\nBCL2L13(W276A/I2... |
67,954 | Live-cell imaging; Calcium imaging with Fura-2 | 4 | dx.doi.org/10.17504/protocols.io.261genzqwg47/v1 | https://www.protocols.io/view/live-cell-imaging-calcium-imaging-with-fura-2-cekstcwe | Minee-Liane Choi | TITLE: Live-cell imaging; Calcium imaging with Fura-2
AUTHORS: Minee-Liane Choi
[DESCRIPTION]
This protocol describes a method to perform calcium imaging with Fura-2 (live-cell imaging)
[STEPS]
1. Cells are washed with HBSS (Cat No. 14025092, ThermofisherScientific)
2. Cells are loaded with 5uM of Fura 2-AM (F1221, ... | ["Cells are washed with HBSS (Cat No. 14025092, ThermofisherScientific)", "Cells are loaded with 5uM of Fura 2-AM (F1221, ThermofisherScientific) in HBSS.", "CCD protocol is set up by selecting a501 nm wheel (both 340 nm and 380 nm wavelength are excited b 501 nm)."] |
83,813 | LB Agar | 4 | dx.doi.org/10.17504/protocols.io.dm6gp38xjvzp/v1 | https://www.protocols.click/view/lb-agar-cv4dw8s6 | sgoodwin | TITLE: LB Agar
AUTHORS: sgoodwin
[DESCRIPTION]
Protocol for producing LB agar growth medium
[STEPS]
SECTION: LB Agar
1. Add 1.5 g LB powder, 1.5 g agarose powder to conical flask
SECTION: LB Agar
2.
Dilute with 200 mL distilled water. Ensure fully dissolved
SECTION: LB Agar
3. Seal conical flask with sponge bung a... | ["[LB Agar] Add 1.5 g LB powder, 1.5 g agarose powder to conical flask", "[LB Agar] Dilute with 200 mL distilled water. Ensure fully dissolved", "[LB Agar] Seal conical flask with sponge bung and foil", "[LB Agar] Sterilise growth medium by autoclaving"] |
84,483 | A modified method to analyse cell proliferation using EdU labelling in large insect brains | 4 | dx.doi.org/10.17504/protocols.io.n92ldmy69l5b/v1 | https://www.protocols.click/view/a-modified-method-to-analyse-cell-proliferation-us-cwrbxd2n | Amaia Alcalde Anton, Max S Farnworth, Laura Hebberecht, Jill Harrison, Stephen H Montgomery | TITLE: A modified method to analyse cell proliferation using EdU labelling in large insect brains
AUTHORS: Amaia Alcalde Anton, Max S Farnworth, Laura Hebberecht, Jill Harrison, Stephen H Montgomery
[DESCRIPTION]
The study of neurogenesis is critical to understanding of the evolution of nervous systems. Within inverte... | ["[0.\tBEFORE YOU START – PREPARE THE STOCK SOLUTIONS] Prepare a 10 mM stock solution of EdU: Add 2 mL of DMSO to EdU, then mix well (store at ≤20 °C).", "[0.\tBEFORE YOU START – PREPARE THE STOCK SOLUTIONS] Prepare a working solution of the Alexa Fluor® azide: Add 70 µL of DMSO to the Alexa Fluor® azide (store at ≤20 ... |
107,088 | Isolation of Nuclei from Brain Tissue Using Gradient Centrifugation for Myelin Depletion for 10x Genomics Platform | 0 | dx.doi.org/10.17504/protocols.io.5qpvok1exl4o/v1 | https://www.protocols.io/view/isolation-of-nuclei-from-brain-tissue-using-gradie-dktq4wmw | Allen Institute | TITLE: Isolation of Nuclei from Brain Tissue Using Gradient Centrifugation for Myelin Depletion for 10x Genomics Platform
AUTHORS: Allen Institute
[DESCRIPTION]
Protocol is used for isolation of nuclei from frozen brain tissue or
thawed and microdissected brain tissue sections for RNA-seq analysis.
Note: Research re... | [] |
24,768 | Buffer Recipes for Protein Purification | null | dx.doi.org/10.17504/protocols.io.4e8gthw | null | Annie Kwon | TITLE: Buffer Recipes for Protein Purification
AUTHORS: Annie Kwon
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Equilibration buffer]
25 mM HEPES, 100 mM NaCl, pH 7.5 ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)1.461 g2.922 g5.844 g11.688 g23.... | ["[Equilibration buffer]\n25 mM HEPES, 100 mM NaCl, pH 7.5 ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)1.461 g2.922 g5.844 g11.688 g23.376 g25 mM HEPES, 500 mM NaCl, pH 7.5 ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 ... |
90,928 | DIMPLE library generation and assembly protocol | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy7k8lx1/v4 | https://www.protocols.io/view/dimple-library-generation-and-assembly-protocol-c42qyydw | Christian Macdonald, David Nedrud, Patrick Rockefeller Grimes, Donovan Trinidad, Catherine Shin, James Fraser, Willow Coyote-Maestas | TITLE: DIMPLE library generation and assembly protocol
AUTHORS: Christian Macdonald, David Nedrud, Patrick Rockefeller Grimes, Donovan Trinidad, Catherine Shin, James Fraser, Willow Coyote-Maestas
[DESCRIPTION]
This is a protocol for generating and QCing mutagenic libraries using the DIMPLE protocol. This version is ... | ["[Preparation] Use DIMPLE to generate mutagenic oligos and primers.", "[Preparation] Important notes: DIMPLE breaks a gene up into sub-library fragments and generates mutagenic insert oligo pools, where each oligo contains barcodes, Type IIS restriction cutsites, and a sub-region of the gene. Be sure to review your li... |
28,945 | Aptamer 2-step conjugation protocol (INSA-Lyon 2016) | null | dx.doi.org/10.17504/protocols.io.8hrht56 | null | Jorge Fernández | TITLE: Aptamer 2-step conjugation protocol (INSA-Lyon 2016)
AUTHORS: Jorge Fernández
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for bioconjugation of amino terminal modified aptamers with carboxyl surface modified latex beads. Adaptated from iGem team INSA-Lyon 2016.</div></div>
[STEP... | ["[Latex Beads Preparation]\nDilute 480 µL of 2.5 % wt beads stock with 120µL of additional PBS buffer. Reaching a final volume of 500 µL.", "[Latex Beads Preparation]\nCentrifugue the tube at 14.000 rpm for 10minutes. Discard the supernatant and resuspend them in 600 µL of MES buffer. For resuspending beads repeated ... |
68,587 | Chromium Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression (CG000505) | 4 | dx.doi.org/10.17504/protocols.io.n2bvj65rwlk5/v1 | https://www.protocols.io/view/chromium-nuclei-isolation-for-single-cell-multiome-ce8jthun | 10x Genomics | TITLE: Chromium Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression (CG000505)
AUTHORS: 10x Genomics
[DESCRIPTION]
The Chromium Nuclei Isolation Kit is an all-in-one solution for the
standardized isolation of nuclei from frozen tissue for use in
10x Genomics Single Cell assays. Frozen tissue samples are
h... | ["[Nuclei Isolation: Tissue Dissociation] Pre-chill centrifuge to 4°C and place reagents and tubes on ice as indicated in the Get Started guide. Label tops and sides of tubes, as well as tops of spin columns, before starting protocol. Perform all protocol steps on ice and centrifugation steps at 4°C.", "[Nuclei Isolati... |
40,986 | Determination of IgG concentration by the Mancini test. | 6 | dx.doi.org/10.17504/protocols.io.bj92kr8e | https://www.protocols.io/view/determination-of-igg-concentration-by-the-mancini-bj92kr8e | Angel Justiz-Vaillant | TITLE: Determination of IgG concentration by the Mancini test.
AUTHORS: Angel Justiz-Vaillant
[STEPS]
?. An appropriate anti-IgG antiserum (antibody) is poured in the center well of an agar-containing plate.
?. Carefully circular wells are cut and detached from the plates.
?. A series of standards containing known co... | ["An appropriate anti-IgG antiserum (antibody) is poured in the center well of an agar-containing plate.", "Carefully circular wells are cut and detached from the plates.", "A series of standards containing known concentrations of IgG are placed in separate wells, while “unknown” human serum samples and control are pla... |
74,407 | CD34+ Cell RNP Nucleofection | 4 | dx.doi.org/10.17504/protocols.io.q26g7y541gwz/v1 | https://www.protocols.io/view/cd34-cell-rnp-nucleofection-ckwfuxbn | Daniel A Kuppers | TITLE: CD34+ Cell RNP Nucleofection
AUTHORS: Daniel A Kuppers
[DESCRIPTION]
Human CD34+hematopoietic stem and progenitor cells (HSPCs) are a standard source of cells for clinical HSC transplantations as well as experimental xenotransplantation to generate “humanized mice”. To further extend the range of applications o... | ["[Synthetic sgRNA reconstitution] Briefly centrifuge your tubes or plates containing synthetic modified single guide RNA (sgRNA) oligos to ensure that the dried RNA pellet is collected at the bottom.", "[Synthetic sgRNA reconstitution] Carefully dissolve sgRNA in the provided nuclease-free 1X TE Buffer (Tris-EDTA, pH ... |
47,758 | nCoV-2019 Illumina Miniseq sequencing protocol (2,000bp amplicon) | 1 | dx.doi.org/10.17504/protocols.io.6qpvrdpjbgmk/v1 | https://www.protocols.io/view/ncov-2019-illumina-miniseq-sequencing-protocol-2-0-bsvnne5e | Bruno Gomez-Gil, juli.encisoi | TITLE: nCoV-2019 Illumina Miniseq sequencing protocol (2,000bp amplicon)
AUTHORS: Bruno Gomez-Gil, juli.encisoi
[DESCRIPTION]
This is a fork of the protocol https://dx.doi.org/10.17504/protocols.io.bh7hj9j6 but modified for tiled 2000bp amplicons, tagmentation with Nextera XT, indexing, and sequencing with the Illumi... | ["[cDNA preparation] We use the \nMix (pipetting) the following components in Eppendorf tube.\n\nComponent Volume\n \nNuclease-free water 4 µL \nGoScriptTM Reaction Buffer, Random Primer 2 µL\nGoScrip... |
69,924 | Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method | 6 | dx.doi.org/10.17504/protocols.io.bp2l6948rlqe/v1 | https://www.protocols.io/view/determination-of-free-and-protein-bound-da-and-ne-cgictuaw | miquel.vila | TITLE: Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
AUTHORS: miquel.vila
[DESCRIPTION]
Protocol for the determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
[STEPS]
SECTION: Preparatio... | ["[Preparation of the aminochrome (AC) external standard] Mix 500 µL of 1 millimolar (mM) with 500 µLof 2 millimolar (mM) disolved in 100 micromolar (µM) aqueous ammonium acetate buffer pH 5.8 at RT with vigorous shaking for 1 min.", "[Preparation of calibration curves] Prepare a stock solution of the IS in 25 millim... |
11,912 | DNA extraction protocol for genome sequencing v2.0 | 1 | dx.doi.org/10.17504/protocols.io.pvgdn3w | https://www.protocols.io/view/dna-extraction-protocol-for-genome-sequencing-v2-0-pvgdn3w | Isabelle Vea, Andrés G. de la Filia, Stevie Bain | TITLE: DNA extraction protocol for genome sequencing v2.0
AUTHORS: Isabelle Vea, Andrés G. de la Filia, Stevie Bain
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Protocol to extract DNA from female mealybug (</span><span style = "font-style:italic;">P.citri</span><span>) for genomic sequenci... | ["[Day 1- 1st part – lysis and proteinase K digestion]\nPreheat heat block/water bath to 56°C. Or if using the a hot chamber with rocking system, turn it on at 56°C.", "From fresh whole sample: Add 3-15 adult females to a 1.5ml Eppendorf tube and add 360μl of Cell Lysis Buffer. Carefully crush females until homogenize... |
44,594 | Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases | 2 | dx.doi.org/10.17504/protocols.io.bpssmnee | https://www.protocols.io/view/small-volume-flow-cytometry-based-multiplex-analys-bpssmnee | Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda | TITLE: Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases
AUTHORS: Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Small, monomeric guanine triphosphate hydrolases (GTPases) are ubiquitous cel... | [] |
99,788 | Library cloning | 0 | dx.doi.org/10.17504/protocols.io.5qpvokrrzl4o/v1 | https://www.protocols.io/view/library-cloning-ddpk25kw | Raining Wang, Melinda Wheelock | TITLE: Library cloning
AUTHORS: Raining Wang, Melinda Wheelock
[DESCRIPTION]
This is the protocol for inserting the library gene of interest into the pre-barcoded vector created in the previous protocol (Barcoded Vector Cloning).
[BEFORE_START]
Ensure there are enough maxi-prep kits available to use.
[STEPS]
SECTIO... | ["[Predigest the barcoded backbone] Set up a 50 µL reaction as outlined below:", "[Predigest the barcoded backbone] Set the thermal cycler for the program below:", "[Predigest the barcoded backbone] Run all digested samples on a 0.5% TBE gel to ensure complete digestion of the backbone.\n\nAlso run1 µg of original star... |
48,705 | Seeking the unicorn | 1 | dx.doi.org/10.17504/protocols.io.bts9nnh6 | https://www.protocols.io/view/seeking-the-unicorn-bts9nnh6 | Edgar Andrade-Lotero, Robert Goldstone | TITLE: Seeking the unicorn
AUTHORS: Edgar Andrade-Lotero, Robert Goldstone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The task is a two-player game in which players interact with 64 tiles arranged in an 8x8 grid. The grid can either hide a unicorn beneath one of the tiles or else it can be abse... | ["Instructions for the experimentOn each round, you and your partner will be shown a grid of 64 squares. Your task is to say whether there is a unicorn hiding behind one of these squares or not.Click on a square to see whether there is a unicorn or if the square is empty. At the bottom of the page you will find buttons... |
31,017 | intracellular calcium assay | 1 | dx.doi.org/10.17504/protocols.io.baihicb6 | https://www.protocols.io/view/intracellular-calcium-assay-baihicb6 | Wakana Shoda, Naohiro Nomura, Fumiaki Ando, Hideaki Tagashira, Takahiro Iwamoto, Akihito Ohta, Kiyosih Isobe, Takayasu Mori, Koichiro Susa, Eisei Sohara, Tatemitsu Rai, Shinichi Uchida | TITLE: intracellular calcium assay
AUTHORS: Wakana Shoda, Naohiro Nomura, Fumiaki Ando, Hideaki Tagashira, Takahiro Iwamoto, Akihito Ohta, Kiyosih Isobe, Takayasu Mori, Koichiro Susa, Eisei Sohara, Tatemitsu Rai, Shinichi Uchida
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol was used... | ["Creation of Loading Buffer: solution A+ solution B■Solution A 5ml Recording Buffer 50µl Probenesid80μl Pluronic F-127 approximate 5 ml pure water (up to 10ml)■Solution B 50 µl DMSO50µℊ Fluo4Loading Buffer should be heated at 37 ℃", "Cultured cells in glass bottom dish should be washed with PBS, and replaced by 1.2 m... |
98,876 | A protocol for computerized quantitative analysis of nerve fibers, mast cells, enteric glial cells and the proximity of mast cells to the nerve fibers in 3D Images of human sigmoid mucosal biopsies | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj3qrlx1/v2 | https://www.protocols.io/view/a-protocol-for-computerized-quantitative-analysis-dcs42wgw | Tao Li, Pu-Qing Yuan, Yvette Taché | TITLE: A protocol for computerized quantitative analysis of nerve fibers, mast cells, enteric glial cells and the proximity of mast cells to the nerve fibers in 3D Images of human sigmoid mucosal biopsies
AUTHORS: Tao Li, Pu-Qing Yuan, Yvette Taché
[DESCRIPTION]
This protocol describes a step-by-step computational wor... | [] |
98,531 | Flow Cytometry ECS Surface Antigens | 4 | dx.doi.org/10.17504/protocols.io.dm6gpwq11lzp/v2 | https://www.protocols.io/view/flow-cytometry-ecs-surface-antigens-dcgb2tsn | Michael Betts, Gregory Golden | TITLE: Flow Cytometry ECS Surface Antigens
AUTHORS: Michael Betts, Gregory Golden
[DESCRIPTION]
High-parameter flow cytometry enables identification and characterization of a wide range of cell populations within a biological sample. Analysis of cell-surface epitopes using a collection of fluorophore-labeled antibodie... | ["[Procedure] Thawing and Resting\n\na. Pre-warm R10 media in a 37 °C water bath. \n\nb. Thaw samples in-vial using a 37 °C water bath.\n\nc. Add thawed cells to 14 mL of R10, then spin cells at 500 xg for 5 min.\n\nd. Resuspend cell pellet in 3 mL of R10 and count cells. \n\ne. Rest cells at least 3 hours (up to overn... |
null | null | null | dx.doi.org/10.17504/protocols.io.hapb2dn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div title="Page 1">
<div>
<div>
<p>Synthetic biology holds great potential for addressing pressing challenges for mankind and our planet. One technical challenge in tapping into the full potential of synthetic biology is the low efficiency and low throughput of genetic transfor... | [] |
53,352 | 2X PEG | 4 | dx.doi.org/10.17504/protocols.io.bycgpstw | https://www.protocols.io/view/2x-peg-bycgpstw | Jacquelina.Woods | TITLE: 2X PEG
AUTHORS: Jacquelina.Woods
[DESCRIPTION]
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA ... | ["Autoclave 121 °C15 min.", "Store at 2-8 Room temperatureprotected from light.", "Dissolve components in ~700 mL deionized or ultrapure water. Heating to 50 °C is recommended to facilitate dissolution.", "Bring total volume up to 1 Lwith deionized or ultrapure water.", "200 g , or equivalent", "34.8 g,, or equivalen... |
36,874 | Preventative at Home COVID-19 Based on Literature | null | dx.doi.org/10.17504/protocols.io.bf9ijr4e | https://www.protocols.io/view/preventative-at-home-covid-19-based-on-literature-bf9ijr4e | Rochelle Lieberstein Stern | TITLE: Preventative at Home COVID-19 Based on Literature
AUTHORS: Rochelle Lieberstein Stern
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Abstract: Are there evidenced-based, reviews, articles of scientific validity, and/or clinical studies that </div><div class = "text-block">imply other actions... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ddn25d | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Collaborator, Nathan VerBerkmoes (Oak Ridge National Labs), worked with Tucson Marine Phage Lab to develop more sensitive proteomics assays for viral isolates which we are now also using for environmental viral concentrates. A new sample prep method (FASP) was optimized to maxim... | [] |
101,756 | Reproduction Analysis | 0 | dx.doi.org/10.17504/protocols.io.kxygxywqdl8j/v1 | https://www.protocols.io/view/reproduction-analysis-dfk43kyw | Núria Peñuelas, Ariadna Laguna, Miquel Vila | TITLE: Reproduction Analysis
AUTHORS: Núria Peñuelas, Ariadna Laguna, Miquel Vila
[DESCRIPTION]
Reproduction Analysis in mice
[STEPS]
1. Register all births and the number of pups per litter, considering heterozygous matings only.
2. Report the mean number of pups per litter. | ["Register all births and the number of pups per litter, considering heterozygous matings only.", "Report the mean number of pups per litter."] |
48,194 | Chimeric Protein-LAG and Peptostreptococcal protein L sandwich ELISA | 1 | dx.doi.org/10.17504/protocols.io.btbaniie | https://www.protocols.io/view/chimeric-protein-lag-and-peptostreptococcal-protei-btbaniie | Angel Justiz-Vaillant | TITLE: Chimeric Protein-LAG and Peptostreptococcal protein L sandwich ELISA
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This ELISA is used to study the interactions between protein-LAG (PLAG) and Peptostreptococcal protein-L (SpL) with different immunoglobulin pr... | ["This ELISA is used to study the interactions between protein-LAG (PLAG) and Peptostreptococcal protein-L (SpL) with different immunoglobulin preparations from mammalian and avian species. The 96 well microtiter plate was coated overnight at 4°C with 2 µg/µl per well of PLAG in carbonate-bicarbonate buffer pH 9.6.", "... |
52,582 | Far East XL Male Enhancement Reviews!! | 1 | dx.doi.org/10.17504/protocols.io.bxkepkte | https://www.protocols.io/view/far-east-xl-male-enhancement-reviews-bxkepkte | health | TITLE: Far East XL Male Enhancement Reviews!!
AUTHORS: health
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><a href="https://www.healthpills24x7.com/order-new-flow-xl-me" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">==> Get Far East XL Male Enhancement for the... | ["(Prodect Name) : Far East XL Male Enhancement(Official Website) : Click Here To Order Now Far East XL Male EnhancementFar East XL is an all-natural male enhancement supplement designed to restore a man’s libido, stamina, and performance in the bedroom.\n\nFor millions of men, their ability to perform in the bedroom ... |
86,090 | Mitophagy induction using Oligomycin/Antimycin A | 1 | null | https://www.protocols.io/view/mitophagy-induction-using-oligomycin-antimycin-a-cybixske | Louise Uoselis | TITLE: Mitophagy induction using Oligomycin/Antimycin A
AUTHORS: Louise Uoselis
[DESCRIPTION]
Mitophagy induction in HeLa cells using Oligomycin/Antimycin A.
[STEPS]
SECTION: Day 1
1. Seed cells, aiming for a confluency of 80-90% at the time of treatment the next day.
SECTION: Day 2
2. Feed cells for 1 h in an appro... | ["[Day 1] Seed cells, aiming for a confluency of 80-90% at the time of treatment the next day.", "[Day 2] Feed cells for 1 h in an appropriate volume of standard growth media.", "[Day 2] To start the treatment, replace the media in each well with standard growth media that contains 10 uM Oligomycin, 4 uM Antimycin A, a... |
64,407 | Tiger Woods Eagle Hemp CBD Gummies | 3 | dx.doi.org/10.17504/protocols.io.q26g749k9gwz/v1 | https://www.protocols.io/view/tiger-woods-eagle-hemp-cbd-gummies-ca5xsg7n | DaidOlier | TITLE: Tiger Woods Eagle Hemp CBD Gummies
AUTHORS: DaidOlier
[DESCRIPTION]
-->Tiger Woods Eagle Hemp CBD Gummies *Updated 2022* 'Reviews' Shocking Results Must Read!
[STEPS] | [] |
87,063 | Combinatorial co-culturing and amplicon sequencing (Cocoa-seq) v1.0 | 4 | null | https://www.protocols.io/view/combinatorial-co-culturing-and-amplicon-sequencing-cy9xxz7n | James Y Tan | TITLE: Combinatorial co-culturing and amplicon sequencing (Cocoa-seq) v1.0
AUTHORS: James Y Tan
[DESCRIPTION]
While synthetic ecology approaches provides phenotypic observations on how interactions
change in different contexts and contribute to overall community function through experimentation by constructing and int... | ["[Droplet co-cultivation in microdroplets] Prepare environment for agarose droplet generation.\n\nAll steps for agarose bead generation should be done in a 37 °C environmental controlled room or in a modified incubator. In our cases, we used a modified large oven incubator (VWR Scientific 1535) to maintain a temperat... |
null | null | null | dx.doi.org/10.17504/protocols.io.nccdasw | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
<p>Software pre-requisites:</p>
<p>Spades</p>
<p>SGA</p>
<p>cutadapt</p>
<p>trim_galore</p>
<p>Augustus</p>
<p>BUSCO</p>
<p> </p>
<p>Data sources:<br /> http://spades.bioinf.spbau.ru/spades_test_datasets/ecoli_sc/</p>
<p> https://www.nature.com/articles/s41598-017-05436-4<br ... | [] |
104,359 | Exploring Uptake and Engagement with Community Food-Related Services in Low Income Areas Using the COM-B Model | 0 | dx.doi.org/10.17504/protocols.io.8epv5rd96g1b/v2 | https://www.protocols.io/view/exploring-uptake-and-engagement-with-community-foo-dh6f39bn | Abigail Stephen, Julia Allan, Oana Petre, Janet Kyle, Frank Thies | TITLE: Exploring Uptake and Engagement with Community Food-Related Services in Low Income Areas Using the COM-B Model
AUTHORS: Abigail Stephen, Julia Allan, Oana Petre, Janet Kyle, Frank Thies
[DESCRIPTION]
Background: Low socioeconomic status (SES) is a significant risk factor for obesity and related non-communicable... | ["[Background and Study Design] Aim:\n\nIn the present study, the aim is to understand and quantify the perspective of users and non-users of community dietary interventions within low-income communities, specifically what barriers and\nfacilitators are associated with their uptake and engagement and consumption of\na ... |
42,054 | Myometrium Single Cell Dissociation Protocol | 1 | dx.doi.org/10.17504/protocols.io.bmbek2je | https://www.protocols.io/view/myometrium-single-cell-dissociation-protocol-bmbek2je | Nicole Ulrich, Yu-Chi Shen, Sue Hammoud | TITLE: Myometrium Single Cell Dissociation Protocol
AUTHORS: Nicole Ulrich, Yu-Chi Shen, Sue Hammoud
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the single cell dissociation of myometrial cells.</div><div class = "text-block"><span>The single cell dissociation protocol fo... | ["[Myometrium single cell dissociation]\nSample arrives in HBSS, at .\n0 Room temperature", "[Myometrium single cell dissociation]\nWeigh and divide sample to -.\n150 mg\n[section of tissue]", "[Myometrium single cell dissociation]\nMake several cuts in thin sections in the tissue (similar to a bivalve for the fallopia... |
98,038 | Test - share with Gabriel p.io | 0 | dx.doi.org/10.17504/protocols.io.e6nvw19kwlmk/v1 | https://www.protocols.io/view/test-share-with-gabriel-p-io-dbyw2pxe | Alberto Martinez | TITLE: Test - share with Gabriel p.io
AUTHORS: Alberto Martinez
[DESCRIPTION]
Abstract for demo protocol
[STEPS]
1. Step 1
2. Step 2
3. Step 3
4. Step 4 | ["Step 1", "Step 2", "Step 3", "Step 4"] |
20,296 | U Mass - Lipid metabolism | null | dx.doi.org/10.17504/protocols.io.x3gfqjw | null | Jason Kim | TITLE: U Mass - Lipid metabolism
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span><span style = "font-weight:bold;"> </span></div><div class = "text-block"><span>
Lipid metabolism is estimated by measuring systemic clearance of [1-</... | ["Survival surgery is performed to establish a chronic indwelling catheter at 5~6 days prior to experiment for intravenous infusion. (refer to M1023: Surgery-jugular vein cannulation)", "Mice are fasted overnight (~15 hours) or for 5 hours prior to the start of experiment.", "Place a mouse in a rat-size restrainer with... |
40,604 | ef | 4 | dx.doi.org/10.17504/protocols.io.bjv4kn8w | https://www.protocols.io/view/ef-bjv4kn8w | Matthew MacKay | TITLE: ef
AUTHORS: Matthew MacKay
[STEPS]
?. sfesf ssefe | ["sfesf ssefe"] |
95,764 | Immunoblotting (Western) Protocol | 0 | dx.doi.org/10.17504/protocols.io.5qpvo3e3bv4o/v1 | https://www.protocols.io/view/immunoblotting-western-protocol-c9ruz56w | Scott Vermilyea | TITLE: Immunoblotting (Western) Protocol
AUTHORS: Scott Vermilyea
[DESCRIPTION]
This protocol details the western blot technique.
[STEPS]
SECTION: Immunoblotting (Western)
1. Run protein lysates using desired Criterion™ TGX™ gels.
SECTION: Immunoblotting (Western)
2. Transfer onto nitrocellulose membranes.
SECTION: ... | ["[Immunoblotting (Western)] Run protein lysates using desired Criterion™ TGX™ gels.", "[Immunoblotting (Western)] Transfer onto nitrocellulose membranes.", "[Immunoblotting (Western)] Detect protein of interest using appropriate primary antibodies at 4 °C 60 min, followed by horseradish peroxidase (HRP)-conjugated sec... |
61,948 | Apple Keto Gummies Australia | 1 | dx.doi.org/10.17504/protocols.io.bp2l613m1vqe/v1 | https://www.protocols.io/view/apple-keto-gummies-australia-b8q4rvyw | soufianeelbekri | TITLE: Apple Keto Gummies Australia
AUTHORS: soufianeelbekri
[DESCRIPTION]
Quite possibly the most well known ways for individual to get in shape and cut back excess is the keto diet. Today, we will enlighten you concerning Apple Keto Gummies Australia diet pills. This new
[STEPS]
1. Quite possibly the most well k... | ["Quite possibly the most well known ways for individual to get in shape and cut back excess is the keto diet. Today, we will enlighten you concerning Apple Keto Gummies Australia diet pills. This new equation is as simple way for anybody to ensure that they see the most ideal outcomes from the ketogenic way of life. I... |
93,186 | Data Analysis Procedures | 5 | null | https://www.protocols.io/view/data-analysis-procedures-c69azh2e | Deziray.Howard | TITLE: Data Analysis Procedures
AUTHORS: Deziray.Howard
[DESCRIPTION]
Draft of the procedures and workflow for data analysis within PDI
[STEPS]
SECTION: Introduction
1. Welcome to the PDI Data Analysis playbook
This document serves as a comprehensive guide to the processes and methodologies followed by our team in d... | ["[Introduction] Welcome to the PDI Data Analysis playbook\n\nThis document serves as a comprehensive guide to the processes and methodologies followed by our team in data analysis to derive meaningful insights and support informed decision-making. We aim to ensure consistency, reliability, and efficiency in our analyt... |
35,944 | Emotional affection on a sustained attention task | 1 | dx.doi.org/10.17504/protocols.io.bfcgjitw | https://www.protocols.io/view/emotional-affection-on-a-sustained-attention-task-bfcgjitw | Luis Pinel, Miguel A. Perez-Nieto, Marta Redondo, Luis Rodríguez-Rodríguez, Fernando Gordillo, Leticia L. Mateos | TITLE: Emotional affection on a sustained attention task
AUTHORS: Luis Pinel, Miguel A. Perez-Nieto, Marta Redondo, Luis Rodríguez-Rodríguez, Fernando Gordillo, Leticia L. Mateos
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol includes the database used in this study. You will find mor... | [] |
64,744 | Tyler Perry CBD Gummies | 3 | dx.doi.org/10.17504/protocols.io.kqdg3p1k1l25/v1 | https://www.protocols.io/view/tyler-perry-cbd-gummies-cbggsjtw | purecbdsale CBD | TITLE: Tyler Perry CBD Gummies
AUTHORS: purecbdsale CBD
[DESCRIPTION]
Tyler Perry CBD Gummies 2022>Scam or Real? Reviews (Read Must)
[STEPS] | [] |
30,929 | Preparing phytoplankton samples for elemental carbon and nitrogen analysis | 1 | null | https://www.protocols.io/view/preparing-phytoplankton-samples-for-elemental-carb-bafribm6 | Jana Hinners, Sinead Collins | TITLE: Preparing phytoplankton samples for elemental carbon and nitrogen analysis
AUTHORS: Jana Hinners, Sinead Collins
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for the preparation of phytoplankton samples for elemental carbon and nitrogen analyses.</div></div>
[STEPS]
?. [Preparing... | ["[Preparing GFF filters]\nWrap up to 20 Whatman GFF filters in aluminium foil and precombust them for 6 hours at 450degrees C, store filters airtight afterwards", "[Preparing GFF filters]\nWeigh precombusted filters and store labelled and individually (e.g. in 6-well microtitre plates)", "[Preparing phytoplankton cult... |
47,603 | The impact of patient-ventilator asynchrony in adult mechanically ventilated patients on outcomes：A systematic review and meta-analysis | 1 | dx.doi.org/10.17504/protocols.io.bsqtndwn | https://www.protocols.io/view/the-impact-of-patient-ventilator-asynchrony-in-adu-bsqtndwn | Michihito Kyo, Tatsutoshi Shimatani, Koji Hosokawa, Shunsuke Taito, Yuki Kataoka, Shinichiro Ohshimo, Nobuaki Shime | TITLE: The impact of patient-ventilator asynchrony in adult mechanically ventilated patients on outcomes：A systematic review and meta-analysis
AUTHORS: Michihito Kyo, Tatsutoshi Shimatani, Koji Hosokawa, Shunsuke Taito, Yuki Kataoka, Shinichiro Ohshimo, Nobuaki Shime
[DESCRIPTION]
<div class = "text-blocks"><div class... | [] |
42,861 | DNA extraction protocol for historical toe pad samples from birds | 4 | dx.doi.org/10.17504/protocols.io.dm6gpwrdplzp/v1 | https://www.protocols.io/view/dna-extraction-protocol-for-historical-toe-pad-sam-bm4mk8u6 | Dave Lutgen, Reto Burri | TITLE: DNA extraction protocol for historical toe pad samples from birds
AUTHORS: Dave Lutgen, Reto Burri
[DESCRIPTION]
Museums hold collections of specimens from vast taxonomic and geographic ranges that constitute rich resources for research into the origins, evolution, and maintenance of biodiversity on earth. Howe... | ["[Tissue preparation] Put toe pad/breast skin tissue (2-3mg) into (use scalpel tip to transfer).", "[Tissue preparation] Mince the tissue with a pointed scalpel blade. Use a new blade for each sample!", "[Digestion] To make sure no tissue is left on the blade, pipet 80 µL of over the scalpel blade, then remove it... |
99,764 | Quantification of IHC Using an Inverted Confocal Microscope and NIS-Elements Program-Killinger Lab 2024 | 0 | dx.doi.org/10.17504/protocols.io.5qpvokrbzl4o/v1 | https://www.protocols.io/view/quantification-of-ihc-using-an-inverted-confocal-m-ddnu25ew | Solji Choi | TITLE: Quantification of IHC Using an Inverted Confocal Microscope and NIS-Elements Program-Killinger Lab 2024
AUTHORS: Solji Choi
[DESCRIPTION]
Protocol to quantify DAB stained sections using NIS-Elements.
[STEPS]
1.
Capture brightfield images with an inverted
confocal microscope with a 20X objective (Nikon A... | ["Capture brightfield images with an inverted\nconfocal microscope with a 20X objective (Nikon A1R).", "Conduct annotation of each tissue section within a bounding box of 2000×2000 pixels for\nmouse tissues and 2863×2454 pixels for human tissues.", "Use a manual RGB-based color thresholding\nalgorithm for mouse tissues... |
93,163 | Salmaso Lab TRAP Adaptation | 1 | dx.doi.org/10.17504/protocols.io.n2bvj311wlk5/v1 | https://www.protocols.io/view/salmaso-lab-trap-adaptation-c68jzhun | Stephanie Simard | TITLE: Salmaso Lab TRAP Adaptation
AUTHORS: Stephanie Simard
[DESCRIPTION]
This protocol details the procedure of Salmaso Lab TRAP Adaptation.
[BEFORE_START]
Day 3: mRNA Extraction & Quantification:
Before beginning; turn on the incubator to 37 °C; and the dry bath to Level 3-5 on the “low” setting. Place the sulfol... | ["[Day 1: Magnetic Bead Preparation] Gather the magnetic beads; pipet the solution up and down to mix the beads.", "[Day 1: Magnetic Bead Preparation] Extract 360 µL of the magnetic beads into a 1.5mL low-bind tube [60 µL per IP]. Place the tube into the tube carriage and place onto the magnet. Wait for the beads to se... |
null | null | null | dx.doi.org/10.17504/protocols.io.c34yqv | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
This protocol comes from a group of other protocols.This protocol is (3) of (4): <br />1. <a href="https://www.protocols.io/view/Large-Volume-Marine-Cyanophage-Phage-Protocols-c3iykd" target="_blank">'Large Volume Marine Cyanophage Phage Protocols'</a><br />2. <a href="https://ww... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ra4d2gw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Four different samples were employed for experiments, designed as follows:</p>
<ol>
<li><u>HABP/HA</u>: cantilever functionalised with hyaluronic acid binding protein, untreated cell sample;</li>
<li><u>BSA/HA</u>: cantilever functionalised with bovine serum albumin, untreate... | ["{\"blocks\":[{\"key\":\"cjrgg\",\"text\":\"AFM cantilevers (OMCL-TR400PSA; spring constant, 0.02 N\\/m; curvature radius of tip, 15 nm; Olympus Co.) are oxidised using an ozone cleaner and submerged in 2% w\\/w (3-Aminopropyl)triethoxysilane\\u00a0\\/ ultra-pure water for 15 minutes to depose (-SH) groups on the prob... |
66,362 | Via Keto Gummies Australia [SPAM & LEGIT] Price, Giveaways, Offers | 1 | dx.doi.org/10.17504/protocols.io.e6nvwkyj9vmk/v1 | https://www.protocols.io/view/via-keto-gummies-australia-spam-amp-legit-price-gi-cc22syge | ViaKeto Gummies | TITLE: Via Keto Gummies Australia [SPAM & LEGIT] Price, Giveaways, Offers
AUTHORS: ViaKeto Gummies
[DESCRIPTION]
What is this new weight reduction supplement F1 Keto ACV Gummies?
Official Website : Click Here
F1 keto gummies is a dietary weight decrease supplement as gummies. The upgrade is figured on a mission t... | [] |
85,162 | iPhone LiDAR tutorial | 1 | dx.doi.org/10.17504/protocols.io.yxmvm21w9g3p/v3 | https://www.protocols.click/view/iphone-lidar-tutorial-cxeixjce | Gregor Luetzenburg | TITLE: iPhone LiDAR tutorial
AUTHORS: Gregor Luetzenburg
[DESCRIPTION]
Recording, exporting and analyzing iPhone LiDAR data
The aim of this tutorial is to provide a guide on how to create models of the earth surface on a small to medium scale with the LiDAR sensor built in the Apple iPhones, edit and export those mo... | ["[Recording data] 3d Scanner App basic functionality\n\nTo record data with the iPhone LiDAR scanner, unlock the iPhone and open the 3d Scanner App. Make sure nothing obstructs the LiDAR scanner at the back of the iPhone. By default, the app opens the ‘camera interface’ which is used for scanning 3D models. While scan... |
null | null | null | dx.doi.org/10.17504/protocols.io.cr2v8d | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the synthesis protocol using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050)
[BEFORE_START]
We strongly recommend wearing gloves and using nuclease-free tubes and reagents to avoid RNase contamination. Reactions are typically 20 μl but can... | [] |
71,403 | Affordable immunofluorescence staining on bones or other tissues without cryostat | 4 | dx.doi.org/10.17504/protocols.io.14egn2zpzg5d/v1 | https://www.protocols.io/view/affordable-immunofluorescence-staining-on-bones-or-chyjt7un | Vincent Fregona, Laetitia Largeaud | TITLE: Affordable immunofluorescence staining on bones or other tissues without cryostat
AUTHORS: Vincent Fregona, Laetitia Largeaud
[DESCRIPTION]
We are happy to share the First UNICORN ★ Protocol on behalf of the UNICORN Hemato.
How to perform affordable immunofluorescence staining on bones or other tissues withou... | ["[Sample preparation] Collect and prepare your samples of choice (femurs, tibiae, spleen, others).\n\nProperly remove any tissue adhering to the bones", "[Tissue Fixation] Fix your samples with 1-2% PFA \n\nComments: 1% for better preservation of Epitopes, 2% makes it easier to cut bones but decreases signal of some A... |
67,397 | Prodentim Australia NZ or Canada Reviews | 1 | dx.doi.org/10.17504/protocols.io.j8nlkkxbwl5r/v1 | https://www.protocols.io/view/prodentim-australia-nz-or-canada-reviews-cd3ds8i6 | (prodentimaustralia) | TITLE: Prodentim Australia NZ or Canada Reviews
AUTHORS: (prodentimaustralia)
[DESCRIPTION]
Prodentim Australia helps reverse acidic processes that create optimal conditions for bacteria. This can help stop deadly bacteria in their tracks, after which the supplement can move on to stage two. The Prodentim Australia s... | [] |
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