id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.vdme246 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is for staining with AbSeq and multiplexing 16 different samples from distinct individuals into the same 10x run.
[GUIDELINES]
Make all necessary preparations for the experiment:
Notify the IHG core that the 10x Controller will be used.
Book the Attune NXT for ce... | ["[Prepare antibody pool] Pool all antibodies being used in this experiment.", "[Thaw Frozen PBMCs] Turn water bath on to 37°C, aliquot ~40 mL of EasySep buffer into a tube, and place in the water bath until it comes to temperature.", "[Thaw Frozen PBMCs] While it warms,\n Get 1 bucket with dry ice and 1 large containe... |
null | null | null | dx.doi.org/10.17504/protocols.io.fxqbpmw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This procedure is optimized for the isolation of 10<sup>7</sup> to 2 x 10<sup>8</sup> cells per tube. If working with fewer than 10<sup>7</sup> cells, keep volumes as indicated for 10<sup>7</sup> cells. For best results, optimize the conditions to your specific cell number an... | [] |
74,000 | Eosin Y | 6 | null | https://www.protocols.io/view/eosin-y-ckhqut5w | joshdm | TITLE: Eosin Y
AUTHORS: joshdm
[DESCRIPTION]
Preparation of stock and dilution of
[STEPS]
SECTION: Prepare 1% Eosin Y stock
1. Weigh 2 g of and add it to a 1000mL flask
SECTION: Prepare 1% Eosin Y stock
2. Add 40 mL of ddwater and swirl to mix
SECTION: Prepare 1% Eosin Y stock
3. add 160 mL of 95% ethano... | ["[Prepare 1% Eosin Y stock] Weigh 2 g of and add it to a 1000mL flask", "[Prepare 1% Eosin Y stock] Add 40 mL of ddwater and swirl to mix", "[Prepare 1% Eosin Y stock] add 160 mL of 95% ethanol and swirl to mix", "[Prepare 1% Eosin Y stock] add 1 mL of Glacial Acetic acid", "[Preparing working dilution of Eosin Y]... |
null | null | null | dx.doi.org/10.17504/protocols.io.rdvd266 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>CAR-T is a kind of cell immunotherapy, and it is currently the most emerging technology to treat tumors with subversive potential. It is also one of the most effective methods for the treatment of malignant tumors.</p>
[STEPS]
?. | [] |
104,368 | Human Knee Tissue Procurement, Resection and Multimodal Analyses | 0 | dx.doi.org/10.17504/protocols.io.kqdg32bp1v25/v1 | https://www.protocols.io/view/human-knee-tissue-procurement-resection-and-multim-dh6q39dw | Merissa Olmer, hswahn, Martin Lotz | TITLE: Human Knee Tissue Procurement, Resection and Multimodal Analyses
AUTHORS: Merissa Olmer, hswahn, Martin Lotz
[DESCRIPTION]
This protocol describes the procurement of entire human knee joints, resection of joint tissues and allocation for multimodal analyses. The protocol is focused on entire knees that are rec... | [] |
43,131 | Protocol to UCSC Genome Browser & BLAST | 5 | null | https://www.protocols.io/view/protocol-to-ucsc-genome-browser-amp-blast-bnc3mayn | TITLE: Protocol to UCSC Genome Browser & BLAST
AUTHORS:
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In this lab, students will work through BLAST and the UCSC Genome browser to find and analyze information about their genes of interest. </div></div>
[STEPS]
?. [UCSC Genome Browser]
Genome Br... | ["[UCSC Genome Browser]\nGenome BrowserYou will now have the opportunity to utilize these tools yourself to investigate a gene that you find interesting. Use these tools to answer the questions below and then relay these in the Lab Results of this Lab Notebook.Find a gene of interest and type it onto the genome browser... | |
92,826 | CPE Culture from Companion Animal Rectal Swabs or Feces | 4 | dx.doi.org/10.17504/protocols.io.rm7vzxe3rgx1/v1 | https://www.protocols.io/view/cpe-culture-from-companion-animal-rectal-swabs-or-c6v2ze8e | Jackie Dietrich, Stephen Cole | TITLE: CPE Culture from Companion Animal Rectal Swabs or Feces
AUTHORS: Jackie Dietrich, Stephen Cole
[DESCRIPTION]
Carbapenemase-producing Enterobacterales (CPE) are one of the most urgent threats to human healthcare globally. Our study aimed at optimizing the chromogenic agar method of screening for CPE with and wit... | ["[CPE Culture from Companion Animal Rectal Swabs or Feces] If starting with feces, dip a cotton tipped applicator into the specimen. The swab does not have to be fully covered with feces, but it should be visibly inoculated. If starting with a rectal swab, proceed to step 2.", "[CPE Culture from Companion Animal Recta... |
81,369 | 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation for sc/sn -- University of Minnesota TMCs (CG000478 Rev A) | 1 | dx.doi.org/10.17504/protocols.io.ewov1opq2lr2/v1 | https://www.protocols.click/view/10x-protocols-chromium-single-cell-nuclei-gene-exp-ctpzwmp6 | 10x Genomics | TITLE: 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation for sc/sn -- University of Minnesota TMCs (CG000478 Rev A)
AUTHORS: 10x Genomics
[DESCRIPTION]
DOIs for dissociation protocols and 10x Genomics fixation for Chromium Single Cell Expression flex protocols.
Protocol ID# (CG) and Revision... | ["https://www.10xgenomics.com/products/single-cell-gene-expression-flex", "10x Protocol CG000478, Rev A (Fixation):"] |
36,837 | Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays | 2 | dx.doi.org/10.17504/protocols.io.bf8djrs6 | https://www.protocols.io/view/protocols-from-sales-et-al-2020-rubisco-activity-c-bf8djrs6 | Cristina Rodrigues Gabriel Sales, Anabela Silva, Elizabete Carmo-Silva | TITLE: Protocols from Sales et al. (2020) Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays
AUTHORS: Cristina Rodrigues Gabriel Sales, Anabela Silva, Elizabete Carmo-Silva
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This collection o... | [] |
25,957 | Field Collecting Protocol for Macrofungi | null | dx.doi.org/10.17504/protocols.io.5kdg4s6 | null | Andrew Wilson | TITLE: Field Collecting Protocol for Macrofungi
AUTHORS: Andrew Wilson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The Sam Mitchel Herbarium of Fungi documents the diversity of “macrofungi” in the Southern Rocky Mountain region. For the use of this collecting protocol, we use the definition of ... | ["Planning and scope of the collecting trip.", "Preparing for the field.", "Describing the survey location so that its physical location and habitat characteristics can be well understood by others.", "Collecting a macrofungal specimen with all the necessary physical structures, associated descriptors, and pictures/tis... |
61,424 | Sequencing of construct | 4 | dx.doi.org/10.17504/protocols.io.bp2l617ydvqe/v1 | https://www.protocols.io/view/sequencing-of-construct-b78qrrvw | Pascale Baden, michela.deleidi | TITLE: Sequencing of construct
AUTHORS: Pascale Baden, michela.deleidi
[DESCRIPTION]
This protocol describes sequencing of construct.
[STEPS]
SECTION: PCR to amplify region of interest
1.
Master mix 1x H2O 13,3 5x Colorless Reaction Buffer (Promega, #M3005) 4 10mM dNTPs (ThermoFisher, #R0182) ... | ["[PCR to amplify region of interest] Master mix 1x H2O 13,3 5x Colorless Reaction Buffer (Promega, #M3005) 4 10mM dNTPs (ThermoFisher, #R0182) 0,4 Fw primer (CMV FW) 0,6 Rv primer (BGH RV) 0,6 GoTaq Polymerase (Promega, #M3005) 0,1 Σ 19µl \n Mix 19 µL MM with 1 µL DNA (50 ng).",... |
35,278 | Electrode Internal Solution with Biocytin II | null | dx.doi.org/10.17504/protocols.io.bepnjdme | null | Allen Institute for Brain Science | TITLE: Electrode Internal Solution with Biocytin II
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This SOP provides instructions to prepare Internal Solution II used for simultaneous patch clamp electrophysiology experiments to probe circuit connectivity.... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ercbd2w | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in <a href="https://www.protocols.io/view/Polyacrylamide-Gel-System-For-Electrophoresis-Of-P-ep7bdrn" target="_blank">Polyacrylamide Gel System For Electrophoresis Of Proteins</a>.
[STEPS]
?.
?.
?. | [] |
69,755 | Reproductive Tissue Collection (Mammals): Post-mortem Sampling | 1 | dx.doi.org/10.17504/protocols.io.q26g7yrb3gwz/v1 | https://www.protocols.io/view/reproductive-tissue-collection-mammals-post-mortem-cgc3tsyn | sanaz.arenivas, Barbara Durrant, comizzolip, mhouck, Jacquelyn Mountcastle, Budhan Pukazhenthi, phil.purdy | TITLE: Reproductive Tissue Collection (Mammals): Post-mortem Sampling
AUTHORS: sanaz.arenivas, Barbara Durrant, comizzolip, mhouck, Jacquelyn Mountcastle, Budhan Pukazhenthi, phil.purdy
[DESCRIPTION]
Version date: January 2023
The following protocol illustrates how to collect and ship living reproductive tissue from ... | ["[Preparation] Proper protective equipment must be worn (gloves, etc). Sterility must be maintained as much as possible.", "[Tissue Collection] Remove the biopsy container from the kit and have them readily available.", "[Tissue Collection] Wet the entire sampling area of the carcass in rubbing alcohol and blot with s... |
18,901 | Protocol for "Enhancing activation in the right temporoparietal junction using theta-burst stimulation: Disambiguating between two hypotheses of top-down control of behavioral mimicry" | null | dx.doi.org/10.17504/protocols.io.wpvfdn6 | null | Korrina A. Duffy, Bruce Luber, R. Alison Adcock, & Tanya L. Chartrand | TITLE: Protocol for "Enhancing activation in the right temporoparietal junction using theta-burst stimulation: Disambiguating between two hypotheses of top-down control of behavioral mimicry"
AUTHORS: Korrina A. Duffy, Bruce Luber, R. Alison Adcock, & Tanya L. Chartrand
[STEPS]
?. | [] |
103,179 | LC-MS/MS Analysis | 0 | dx.doi.org/10.17504/protocols.io.q26g71388gwz/v1 | https://www.protocols.io/view/lc-ms-ms-analysis-dgzj3x4n | Shiyi Wang | TITLE: LC-MS/MS Analysis
AUTHORS: Shiyi Wang
[DESCRIPTION]
LC-MS/MS Analysis
[STEPS]
1. **Sample Injection** - Inject 3 μL of each sample into the MClass UPLC system (Waters Corp).
2. **Sample Trapping** - Trap the sample on a Symmetry C18 20 mm × 180 μm trapping column at a flow rate of 5 μL/min with a solvent compo... | ["**Sample Injection** - Inject 3 μL of each sample into the MClass UPLC system (Waters Corp).", "**Sample Trapping** - Trap the sample on a Symmetry C18 20 mm × 180 μm trapping column at a flow rate of 5 μL/min with a solvent composition of 99.9/0.1 v/v water/acetonitrile.", "**Analytical Separation** - Perform analyt... |
96,374 | Dispensing (non-L1) C. elegans to 96 well imaging plates using Integra VIAFILL | 4 | dx.doi.org/10.17504/protocols.io.rm7vzxwj2gx1/v1 | https://www.protocols.io/view/dispensing-non-l1-c-elegans-to-96-well-imaging-pla-dacw2axe | e.warren Warren, Bonnie Evans | TITLE: Dispensing (non-L1) C. elegans to 96 well imaging plates using Integra VIAFILL
AUTHORS: e.warren Warren, Bonnie Evans
[DESCRIPTION]
Protocol for dispensing later larval stage C. elegans into 96 well plates using the Interga VIAFILL dispenser. A bleach synchronised C. elegans population should be "re-fed" onto a... | ["[Configure the Integra VIAFILL] Insert a small cassette into the machine.", "[Configure the Integra VIAFILL] Create a new program (or use existing program) with the following settings:", "[Configure the Integra VIAFILL] Mode: Repeat Dispense\nTubing: 8 channel small\nPlate: 96 \nVolume: 10 μl\nMap: Full\nSpeed: Fast"... |
null | null | null | dx.doi.org/10.17504/protocols.io.qnndvde | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>BACKGROUND</p>
<p>Tuberculosis (TB) represents the ninth leading cause of death worldwide. In 2016, are estimated 1.3 million TB deaths among HIV negative people and an additional 374,000 deaths among HIV positive people.In2016, are estimated 1.4 million new cases of TB in pe... | [] |
19,254 | Chinese Proprietary Medicine for Helicobacter pylori infection: a network meta-analysis | null | dx.doi.org/10.17504/protocols.io.w2wfgfe | null | Xiao-bei Si, Shuai Wang, Xu-min Zhang, Yu Lan | TITLE: Chinese Proprietary Medicine for Helicobacter pylori infection: a network meta-analysis
AUTHORS: Xiao-bei Si, Shuai Wang, Xu-min Zhang, Yu Lan
[STEPS]
?. | [] |
59,523 | JAX SCBL workflow for scRNA-seq of human placenta | 1 | dx.doi.org/10.17504/protocols.io.eq2lynw6pvx9/v1 | https://www.protocols.click/view/jax-scbl-workflow-for-scrna-seq-of-human-placenta-b6dbra2n | Diane Luo, Jessica Grassmann, Paul Gabriel, William F Flynn, Elise Courtois, Paul Robson | TITLE: JAX SCBL workflow for scRNA-seq of human placenta
AUTHORS: Diane Luo, Jessica Grassmann, Paul Gabriel, William F Flynn, Elise Courtois, Paul Robson
[DESCRIPTION]
Described here is the workflow used by the JAX Single Cell Biology lab to generate single cell transcriptomic libraries from human placenta samples co... | ["[Tissue collection] Tissue is collected and processed as soon as possible after Cesarean section or vaginal delivery according to the following protocol:\n\nHuman Placenta Tissue Collection and Preservation Methods - UCSD Female Reproductive TMC\n\nTissue is then stored in MACS storage buffer for overnight shipping."... |
50,981 | RNA Extraction Full Protocol | 4 | null | https://www.protocols.io/view/rna-extraction-full-protocol-bv2dn8a6 | Onkar Nath, Stephen Fletcher, Alice Hayward, Agnelo Furtado, Robert J Henry, Neena Mitter | TITLE: RNA Extraction Full Protocol
AUTHORS: Onkar Nath, Stephen Fletcher, Alice Hayward, Agnelo Furtado, Robert J Henry, Neena Mitter
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">RNA Extraction protocol suitable for all Avocado tissues. This protocol also suits extraction from many other plants ... | ["[Tissue Lysis]\nFinely grind frozen tissue. Grinding in the buffer is not recommended.", "[Tissue Lysis]\nPreparation on the Day of UseAdd 10% SDS per 2ml OB1 buffer and β-mercaptoethanol (Fume-hood should be used for working with 2ME)Heat the buffer to .\n10 µl\n65 °C", "[Tissue Lysis]\nAdd approximately (0.01 g)... |
60,562 | Stranded Transcript Count Table Generation from Long Reads | 1 | dx.doi.org/10.17504/protocols.io.5qpvonn2bl4o/v14 | https://www.protocols.io/view/stranded-transcript-count-table-generation-from-lo-b7dsri6e | David A Eccles | TITLE: Stranded Transcript Count Table Generation from Long Reads
AUTHORS: David A Eccles
[DESCRIPTION]
This protocol is for comparing different samples at the transcript level, using long reads that are mapped to transcripts.
Input(s): demultiplexed and oriented fastq files (see protocol Preparing Reads for Strande... | ["[Demultiplex Reads] Demultiplex and orient reads as per the protocol Preparing Reads for Stranded Mapping. It is expected that these demultiplexed reads will be split up in the current directory, and coupled with a 'barcode_counts.txt' file. If that's the case, the following should work:\n \nExample expected output:\... |
25,295 | Quadruple Retrograde Tracing | null | dx.doi.org/10.17504/protocols.io.4xpgxmn | null | Hongwei Dong | TITLE: Quadruple Retrograde Tracing
AUTHORS: Hongwei Dong
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"> Stereotaxic surgeries are performed to inject neural circuit tracers into different target regions in the brain.</div></div>
[STEPS]
?. Instruments are washed with soap, wiped down with alcoho... | ["Instruments are washed with soap, wiped down with alcohol, and autoclaved for the first surgery. Instruments are wiped down with alcohol and sterilized via a glass bead sterilizer in between surgeries.", "Animal is deeply anesthetized. The surgeries are performed under isoflurane anesthesia. Mice are initially anesth... |
null | null | null | dx.doi.org/10.17504/protocols.io.mbzc2p6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Brief description about the <strong>Age-adjusted Charlson comorbidity index </strong></p>
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.mf2c3qe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to... | [] |
18,904 | Test-mate (Model 400) erythrocyte acetylcholinesterase (AChE) test | null | dx.doi.org/10.17504/protocols.io.wpyfdpw | null | H.K. Jeevan Dhanarisi, Indika B. Gawarammana, Fahim Mohamed, Michael Eddleston | TITLE: Test-mate (Model 400) erythrocyte acetylcholinesterase (AChE) test
AUTHORS: H.K. Jeevan Dhanarisi, Indika B. Gawarammana, Fahim Mohamed, Michael Eddleston
[STEPS]
?. [Erythrocyte AChE activity analysis]
Remove Test-MateTM components from the carrying case and lay them out, within easy reach, in front of you on... | ["[Erythrocyte AChE activity analysis]\nRemove Test-MateTM components from the carrying case and lay them out, within easy reach, in front of you on a flat surface.", "Press the “Power” key. The display screen indicates a 15 seconds warm up count down. Write up the temperature on your sheet.", "Press the “Mode” key un... |
106,444 | Viral Enumeration of Water Samples Using Wet Mount Epifluorescence Microscopy | 0 | null | https://www.protocols.io/view/viral-enumeration-of-water-samples-using-wet-mount-dj7k4rkw | Madeline Bellanger, Pieter Visscher, Richard Allen White III | TITLE: Viral Enumeration of Water Samples Using Wet Mount Epifluorescence Microscopy
AUTHORS: Madeline Bellanger, Pieter Visscher, Richard Allen White III
[DESCRIPTION]
Epifluorescence microscopy (EFM) has been the gold standard method for environmental viral enumeration for over 25 years. Currently, standard EFM meth... | ["[Filtration and Cleaning] Record the starting volume of water, this will be used for calculations later.", "[Filtration and Cleaning] Filter water through a glass fiber pre filter (Whatman Grade 1 qualitative filter paper).", "[Filtration and Cleaning] Collect filtrate and filter through a 0.65 μm PVDF filter (Durapo... |
88,187 | MSI-Explorer - a napari plug-in for biochemical annotation of mass spectrometry imaging data | 5 | dx.doi.org/10.17504/protocols.io.n2bvj3dyplk5/v1 | https://www.protocols.io/view/msi-explorer-a-napari-plug-in-for-biochemical-anno-c2c3yayn | Nay Min Min Thaw Saw, Lennart Kowitz, Peter Lampen, Karl W Smith, Jianxu Chen, PRASAD PHAPALE | TITLE: MSI-Explorer - a napari plug-in for biochemical annotation of mass spectrometry imaging data
AUTHORS: Nay Min Min Thaw Saw, Lennart Kowitz, Peter Lampen, Karl W Smith, Jianxu Chen, PRASAD PHAPALE
[DESCRIPTION]
Mass spectrometry imaging (MSI) provides label-free localization of hundreds of biochemicals — metabol... | ["[Uploading and visualization of MSI data] In anaconda prompt, create the environment by typing this.\n\n \n\nIn this case, “napari-env” is the name of the environment. Any name can be given.", "[Uploading and visualization of MSI data] Then, activate the environment.", "[Uploading and visualization of MSI data] Insta... |
64,771 | Beetle rearing media | 1 | dx.doi.org/10.17504/protocols.io.eq2lyn48rvx9/v1 | https://www.protocols.io/view/beetle-rearing-media-cbhbsj2n | Tina, Yin-Tse Huang | TITLE: Beetle rearing media
AUTHORS: Tina, Yin-Tse Huang
[DESCRIPTION]
ambrosia beetle rearing
[STEPS]
2. First, add dry ingredients
6. Put a double-layered cotton gauze on the mouth of the filled tubes. The cap is pre-drilled with holes that allow aeration and prevent contamination.
7. Autoclave the fil... | ["First, add dry ingredients", "Put a double-layered cotton gauze on the mouth of the filled tubes. The cap is pre-drilled with holes that allow aeration and prevent contamination.", "Autoclave the filled tube at 121 °C for 30 min.", "Put autoclved tubes in an oven at 55°C for two days. (cotton gauze and cap remains on... |
null | null | null | dx.doi.org/10.17504/protocols.io.cppvmm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is for pSerine and pThreonine western blotting
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kavcse6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Background</strong></p>
<p>Recently, neoadjuvant chemotherapy with docetaxel/cisplatin/5-fluorouracil (NAC-DCF) was identified as a novel strong regimen with a high rate of pathological complete response (pCR) in advanced esophageal cancer in Japan. Predicting pCR wil... | [] |
72,222 | Cell line information | 1 | dx.doi.org/10.17504/protocols.io.rm7vz82o8vx1/v2 | https://www.protocols.io/view/cell-line-information-cir6ud9e | Philippa R Kennedy | TITLE: Cell line information
AUTHORS: Philippa R Kennedy
[DESCRIPTION]
An amalgamation of cell line data in the lab. Its origin and standard culture conditions.
[STEPS]
SECTION: Overview
1. All cells are maintained in humidified incubators at 37°C, 5% CO2, unless otherwise specified.
All cells are routinely tested... | ["[Overview] All cells are maintained in humidified incubators at 37°C, 5% CO2, unless otherwise specified. \n\nAll cells are routinely tested for mycoplasma infection using a PCR-based test (Universal Mycoplasma Detection Kit, ATCC Cat. No. 30-1012K)\n\nDates of purchase are provided. Aliquots of purchased cells lines... |
65,236 | Myco Nootropic Brain Gummies Reviews – Does This Product Really Work? | 3 | dx.doi.org/10.17504/protocols.io.yxmvmn13ng3p/v1 | https://www.protocols.io/view/myco-nootropic-brain-gummies-reviews-does-this-pro-cbxuspnw | olgasimons | TITLE: Myco Nootropic Brain Gummies Reviews – Does This Product Really Work?
AUTHORS: olgasimons
[DESCRIPTION]
Myco Nootropic Brain Gummies
[STEPS] | [] |
45,320 | 3.5 Doxycycline-Induced Differentiation | 1 | dx.doi.org/10.17504/protocols.io.bqhgmt3w | https://www.protocols.io/view/3-5-doxycycline-induced-differentiation-bqhgmt3w | Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp | TITLE: 3.5 Doxycycline-Induced Differentiation
AUTHORS: Evelyn J. Sauter, Lisa K. Kutsche, Simon D. Klapper, Volker Busskamp
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is part 3.3 of the "</span><a href="https://www.protocols.io/private/C65DB81838BC11EBA61A0A58A9FEAC2A/protocols" sty... | ["[3.5 Doxycycline-Induced Differentiation]\nNeurons can be grown on Matrigel-coated cell culture dishes, however, especially for long-term neuronal differentiation, it is recommended to grow the neurons on cell culture plates coated with poly-L-lysine (PLL) and laminin. Dilute the PLL in ddH2O to a final concentration... |
69,198 | Single strain culture DNA extractions with polyzyme | 1 | dx.doi.org/10.17504/protocols.io.rm7vzbm3xvx1/v1 | https://www.protocols.io/view/single-strain-culture-dna-extractions-with-polyzym-cftntnme | Jason Laurich, o.pogoutse, Megan Frederickson | TITLE: Single strain culture DNA extractions with polyzyme
AUTHORS: Jason Laurich, o.pogoutse, Megan Frederickson
[DESCRIPTION]
Protocol for DNA extractions from single strain bacterial cultures using the MetaPolyzyme Multilytic Enzyme mix (SIgma)
[STEPS]
SECTION: Bacterial culturing
1. - Place cells from agarose pla... | ["[Bacterial culturing] - Place cells from agarose plates with single strain growing on it (preferably from single colony) into liquid culture. \n\n- Culture cells at appropriate temperature (27-30 C traditionally) for 1-4 days, as appropriate.", "[Pelleting cells] - Centrifuge 1 mL of liquid bacterial culture for 2 mi... |
null | null | null | dx.doi.org/10.17504/protocols.io.ua8eshw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
The protocol outlines how to aliquot PCR Master Mix for 5µL miniaturized amplification of 16S gene in 384-well format using the epMotion.
[BEFORE_START]
Please wear at least the minimum required personal protective equipment.
Ensure that all necessary kit components are availab... | ["[Mix reagents] {\"blocks\":[{\"key\":\"a2hfn\",\"text\":\"In a sterile 10mL reservoir, add 2,534\\u00a0\\u03bcl of Platinum Hot Start PCR 2X Master Mix and 3,295 \\u00b5L of PCR Clean Water per set of 384 samples\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[],\"entityRanges\":[],\"data\":[]},{\"key\":\"... |
85,944 | CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP | 1 | dx.doi.org/10.17504/protocols.io.kqdg3x99eg25/v1 | https://www.protocols.io/view/crispr-tagging-of-the-eea1-gene-in-h9-es-cells-for-cx6yxrfw | Jiuchun Zhang, Harper JW | TITLE: CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP
AUTHORS: Jiuchun Zhang, Harper JW
[DESCRIPTION]
This protocol describes a method for knockin of a 3X flag tag onto the N-terminus of the EEA1 gene in human H9 ES cells using CAS9 and an ultramer oligonucleotide homologous recombination template
[BEFOR... | ["[preparing Cas9 RNP complex for electroporation] Add 10 µL to a sterile 1.5 ml tube. Add 6 µg. Then add 1.2 µg. Pipet up and down to mix. Let it sit at Room temperature for 10 min. This is enough for 2 transfections (== one 6 well).", "[preparing Cas9 RNP complex for electroporation] While waiting for the Cas9 to bin... |
71,329 | Generation of glioblastoma spheroid model using an orbital shaker | 1 | dx.doi.org/10.17504/protocols.io.8epv5jz86l1b/v1 | https://www.protocols.io/view/generation-of-glioblastoma-spheroid-model-using-an-chv9t696 | WANNAWAT KHOTCHAWAN, Chanchao Lorthongpanich, Pakpoom Kheolamai, Sith Sathornsumetee, Surapol Issaragrisil | TITLE: Generation of glioblastoma spheroid model using an orbital shaker
AUTHORS: WANNAWAT KHOTCHAWAN, Chanchao Lorthongpanich, Pakpoom Kheolamai, Sith Sathornsumetee, Surapol Issaragrisil
[DESCRIPTION]
Glioblastoma multiforme (GBM) is the most common and aggressive type of primary brain tumor with no effective treatm... | ["[Generation of three-dimensional (3D) glioblastoma spheroids] Culture the glioblastoma cell line (U251-MG) in the U251-MG media (DMEM-high glucose medium containing 10% fetal bovine serum) on a 75-cm2 cell culture flask for 3–4 days till the cell reach 70–80% confluence before collected for spheroid formation.", "[Ge... |
57,367 | Collection of human vagal tissue samples for TEM imaging | 1 | dx.doi.org/10.17504/protocols.io.b39xqr7n | https://www.protocols.io/view/collection-of-human-vagal-tissue-samples-for-tem-i-b39xqr7n | Terry Powley, Deborah Jaffey, Anita Gupta, Plamen Mihaylov, John Wo | TITLE: Collection of human vagal tissue samples for TEM imaging
AUTHORS: Terry Powley, Deborah Jaffey, Anita Gupta, Plamen Mihaylov, John Wo
[DESCRIPTION]
This protocol describes the methods used to generate samples of human vagus tissue suitable for TEM imaging.
[STEPS]
SECTION: Sampling
1. Vagus nerve samples wer... | ["[Sampling] Vagus nerve samples were obtained intra-operatively from organ donors. Separate consents for organ donation and research were obtained. Informed consent for study participation was obtained from a parent and/or legal guardian. No organ donors were prisoners. A diagnosis of brain death or circulatory death ... |
71,166 | Preparation of frozen nuclei for single-nucleus RNA seq | 1 | dx.doi.org/10.17504/protocols.io.eq2lyrdpgx9k/v2 | https://www.protocols.io/view/preparation-of-frozen-nuclei-for-single-nucleus-rn-chq6t5ze | Kimberly Siletti, Sten Linnarsson | TITLE: Preparation of frozen nuclei for single-nucleus RNA seq
AUTHORS: Kimberly Siletti, Sten Linnarsson
[DESCRIPTION]
This protocol describes our preparation of nuclei for single-nucleus RNA sequencing on the 10X Genomics Chromium system, starting from FAC-sorted frozen nuclei.
[STEPS] | [] |
101,136 | Making Ca2+ - selective microelectrodes | 0 | dx.doi.org/10.17504/protocols.io.yxmvmeeqog3p/v1 | https://www.protocols.io/view/making-ca2-selective-microelectrodes-dezq3f5w | Jeff Stedehouder, Dorly Verdier, Arlette Kolta, Stephanie J Cragg | TITLE: Making Ca2+ - selective microelectrodes
AUTHORS: Jeff Stedehouder, Dorly Verdier, Arlette Kolta, Stephanie J Cragg
[DESCRIPTION]
The following protocol describes how to make Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices. Gl... | ["[Silanization]", "[Calibration] Electrodes should be calibrated at experimental temperatures (~33°C) with standard aCSF solutions containing increasing concentrations of Ca2+ (0, 0.1, 0.2, 0.4, 0.8, 1.2, 1.6 and 2 mM), the potentials for each concentration recorded, and a calibration curve obtained fitted with a loga... |
null | null | null | dx.doi.org/10.17504/protocols.io.infcdbn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Gen-IALFirst All-tissue DNA extraction kit -This protocol provides an efficient DNA extraction and purification of historic sample (tissue material)</p>
[BEFORE_START]
<p>Ancient DNA lab</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
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?.
?. ... | [] |
44,066 | Postnatal ventral midbrain dopamine neuronal culture protocols | 4 | dx.doi.org/10.17504/protocols.io.bpaamiae | https://www.protocols.io/view/postnatal-ventral-midbrain-dopamine-neuronal-cultu-bpaamiae | Dave Sulzer, Ellen Kanter | TITLE: Postnatal ventral midbrain dopamine neuronal culture protocols
AUTHORS: Dave Sulzer, Ellen Kanter
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the specific steps and media solutions required to dissect and triturate the postnatal netral midbrain dopamine neuronal cul... | ["[Aclar Dishes for Electron Microscope Preparations]\nCut Aclar sheets into squares ~ 1.5 cm and drop into Nanopure dd H2O for .", "[Aclar Dishes for Electron Microscope Preparations]\nSwirl the squares in a beaker (if they don’t all immediately float to the top).", "[Aclar Dishes for Electron Microscope Preparations]... |
23,237 | Making water stress treatments in pot experiments: An illustrated step-by-step guide | null | dx.doi.org/10.17504/protocols.io.2xdgfi6 | null | Matema LE. Imakumbili | TITLE: Making water stress treatments in pot experiments: An illustrated step-by-step guide
AUTHORS: Matema LE. Imakumbili
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">This protocol is a step-by-step guide on how to make water stress treatments in ... | ["Determining the moisture content of the potting soilAir dry soil even when thoroughly air-dried always has moisture no matter how little. This moisture has to be accounted for when determining the amount of water needed to bring a soil to its field capacity. The first step thus involves the determination of the potti... |
86,921 | Collect and Extract Biomass Cyanobacteria | 1 | dx.doi.org/10.17504/protocols.io.ewov1qqpkgr2/v1 | https://www.protocols.io/view/collect-and-extract-biomass-cyanobacteria-cy5hxy36 | Ricardo M. Borges, Fernanda Chagas | TITLE: Collect and Extract Biomass Cyanobacteria
AUTHORS: Ricardo M. Borges, Fernanda Chagas
[DESCRIPTION]
Protocolo Abrangente para a Extração de Biomassa de Cianobactérias Cultivadas em Laboratório.
É crucial prestar uma atenção meticulosa a materiais de qualidade, envolvendo solventes de grau HPLC, microtubos imac... | ["[Coleta da Biomassa] Após 28 dias, extraia ~80-90% de biomassa de cada frasco Erlenmeyer (quantidade determinada apenas visualmente) \nPermita que os espécimes cresçam até formar uma boa quantidade de biomassa, com um limite tentativo empírico de quatro meses por espécime.\n\n> Será permitido que cada espécime se man... |
44,089 | RMP | 1 | dx.doi.org/10.17504/protocols.io.bpazmif6 | https://www.protocols.io/view/rmp-bpazmif6 | Akshita Varma | TITLE: RMP
AUTHORS: Akshita Varma
[STEPS]
?. [How to prepare for Risk Management (PMI-RMP) Certification?]
The candidates are required to analyse the issues properly and plan solutions accordingly to deal with the situation. The following completely depends upon the analysing quality and experience of an individual. T... | ["[How to prepare for Risk Management (PMI-RMP) Certification?]\nThe candidates are required to analyse the issues properly and plan solutions accordingly to deal with the situation. The following completely depends upon the analysing quality and experience of an individual. The candidates also need to keep in mind tha... |
null | null | null | dx.doi.org/10.17504/protocols.io.p3kdqkw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Building predictive sensors is of paramount importance in both biology and science. Can we make a randomly wired sensor ``good enough'' at predicting its input simply by making it larger? We show that infinitely large, randomly wired sensors are nonspecific for their input,... | [] |
70,760 | protocol test redesign 01 | 1 | null | https://www.protocols.io/view/protocol-test-redesign-01-chcgt2tw | Maria Gul, katarina | TITLE: protocol test redesign 01
AUTHORS: Maria Gul, katarina
[DESCRIPTION]
Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Integer in sapien. Praesent id justo in neque elementum ultrices. Cras elementum. Sed ac dolor sit amet purus malesuada congue. Nunc auctor. Quis autem vel eum iure reprehenderit qui in... | ["Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Integer in sapien. Praesent id justo in neque elementum ultrices. Cras elementum. Sed ac dolor sit amet purus malesuada congue. Nunc auctor. Quis autem vel eum iure reprehenderit qui in ea voluptate velit esse quam nihil molestiae consequatur, vel illum qui do... |
null | null | null | dx.doi.org/10.17504/protocols.io.d4e8td | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Method used to perform quality control on Illumina sequences - includes adapter/primer removal and quality trimming<br />Goal: Remove low quality sequences to increase average quality score of reads above 28
[BEFORE_START]
**There are many tools available for this process - thi... | [] |
86,808 | CAMbank: CPT Field Processing v1 | 4 | dx.doi.org/10.17504/protocols.io.kxygx338kg8j/v1 | https://www.protocols.io/view/cambank-cpt-field-processing-v1-cyzyxx7w | Eliah G Overbey, Krista A Ryon, JangKeun Kim, Christopher E Mason | TITLE: CAMbank: CPT Field Processing v1
AUTHORS: Eliah G Overbey, Krista A Ryon, JangKeun Kim, Christopher E Mason
[DESCRIPTION]
Field processing of CPT vacutainers for the Cornell Aerospace Medicine Biobank (CAMbank).
Instructions for preserving: Plasma, PBMCs, and RBC Pellets.
[STEPS]
SECTION: Perform Venipuncture... | ["[Perform Venipuncture] After venipuncture, invert the tubes 8 to 10 times to fully mix in the sodium heparin anticoagulant.\n\nStore the tube upright at room temperature until centrifugation.\n\nTo ensure proper barrier formation, blood samples should be centrifuged within 2 hours of blood collection. Centrifugation ... |
108,714 | Relative Telomere Length Measurement | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1rn7lmk/v1 | https://www.protocols.io/view/relative-telomere-length-measurement-dnei5bce | Sinan Gültekin | TITLE: Relative Telomere Length Measurement
AUTHORS: Sinan Gültekin
[DESCRIPTION]
Relative Telomere Length Measurement by qPCR
[STEPS]
SECTION: Relative Telomere Length Measurement
1. DNA isolation
SECTION: Relative Telomere Length Measurement
1.1. Genomic
DNA was extracted using the DNeasy Blood and Tissue Kit
(Qiag... | ["[Relative Telomere Length Measurement] DNA isolation", "[Relative Telomere Length Measurement] Genomic\nDNA was extracted using the DNeasy Blood and Tissue Kit\n(Qiagen, Hilden, Germany", "[qPCR] 10 ng/μL DNA was amplified in a 5x EvaGreen‱ mix (No ROX) (Bio&Sell, Nurnberg, Germany). The thermal cycling protocol of ... |
67,837 | Support Protocol 2: Metagenotyping with a Custom Collection of Genomes | 5 | dx.doi.org/10.17504/protocols.io.dm6gpbp65lzp/v1 | https://www.protocols.io/view/support-protocol-2-metagenotyping-with-a-custom-co-ceg5tby6 | miriam.goldman , chunyu.zhao | TITLE: Support Protocol 2: Metagenotyping with a Custom Collection of Genomes
AUTHORS: miriam.goldman , chunyu.zhao
[DESCRIPTION]
This protocol describes how to build a MIDASDB from a custom collection of genomes and perform SNV metagenotyping with it. Other MIDAS2 commands can also be run with the new database. Si... | ["Download the example genome collection folder from Zenodo to the work directory (midas2_protocol)\n \n\n \nMIDAS2 reserves the --midasdb_name newdb for building any new MIDASDB, and the custom MIDASDB will be built at --midasdb_dir midasdb_custom", "Construct rep-genome component of MIDASDB\nAnnotate all the genomes ... |
30,712 | ELISPOT Protocol | null | dx.doi.org/10.17504/protocols.io.98yh9xw | null | Sam Li | TITLE: ELISPOT Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Prepare the Plate:]
Prepare the PVDF membrane 96-well ELISPOT plates (e.g., Millipore Cat. No. MAIPS-4510) by soaking them in 35% ethanol for 30 seconds.
?. [Prepare the Plate:]
Wash thoroughly with PBS to remove any r... | ["[Prepare the Plate:]\nPrepare the PVDF membrane 96-well ELISPOT plates (e.g., Millipore Cat. No. MAIPS-4510) by soaking them in 35% ethanol for 30 seconds.", "[Prepare the Plate:]\nWash thoroughly with PBS to remove any residual ethanol.Note: Ethanol can negatively affect cell viability and antibody binding.", "[Coat... |
31,402 | Gibson Assembly Cloning | null | dx.doi.org/10.17504/protocols.io.bawiifce | null | Addgene the nonprofit plasmid repository | TITLE: Gibson Assembly Cloning
AUTHORS: Addgene the nonprofit plasmid repository
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes Gibson Assembly cloning (</span><a href="http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1318.html" style = "text-decoration:underline;co... | ["Design your plasmid and order primers (see figure below).When designing your plasmid, think about what DNA segments you will need to join to create your final plasmid. Adjacent segments should have identical sequences on the ends (sequences A and B in the figures). These identical sequences can be created via PCR wit... |
96,494 | PILOT, OPEN NON-CONTROLLED TRIAL TO ASSESS THE FEASIBILITY OF IMPLEMENTING OBJECTIVE PARAMETERS AS PRIMARY ENDPOINTS IN A CLINICAL TRIAL WITH PATIENTS AFFECTED BY KNEE OSTEOARTHRITIS. | 0 | dx.doi.org/10.17504/protocols.io.n2bvj35o5lk5/v2 | https://www.protocols.io/view/pilot-open-non-controlled-trial-to-assess-the-feas-dagn2bve | Bogdan-Corneliu Andor1, Dionisio Franco Barattini2, Simone Guadagna3, Luca Barattini4, Serban Rosu1, Dumitru-Emanuel Dogaru2 | TITLE: PILOT, OPEN NON-CONTROLLED TRIAL TO ASSESS THE FEASIBILITY OF IMPLEMENTING OBJECTIVE PARAMETERS AS PRIMARY ENDPOINTS IN A CLINICAL TRIAL WITH PATIENTS AFFECTED BY KNEE OSTEOARTHRITIS.
AUTHORS: Bogdan-Corneliu Andor1, Dionisio Franco Barattini2, Simone Guadagna3, Luca Barattini4, Serban Rosu1, Dumitru-Emanuel Dog... | [] |
44,829 | Metabolomic analysis | 3 | null | https://www.protocols.io/view/metabolomic-analysis-bpz5mp86 | Torsten Thomas | TITLE: Metabolomic analysis
AUTHORS: Torsten Thomas
[STEPS] | [] |
24,577 | Targeted absolute transcript quantification in single cells after whole transcriptome amplification | 1 | dx.doi.org/10.17504/protocols.io.389grz6 | https://www.protocols.io/view/targeted-absolute-transcript-quantification-in-sin-389grz6 | Franziska Durst, Ana Grujovic, Iris Ganser, Martin Hoffmann, Peter Ugocsai, Christoph A. Klein, Zbigniew T. Czyż | TITLE: Targeted absolute transcript quantification in single cells after whole transcriptome amplification
AUTHORS: Franziska Durst, Ana Grujovic, Iris Ganser, Martin Hoffmann, Peter Ugocsai, Christoph A. Klein, Zbigniew T. Czyż
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Gene expression ... | ["[Single cell isolation]\nSingle cells are manually isolated with a micromanipulator (Eppendorf, PatchMan NP2) as previously described (Hartmann CH, Klein CA. Nucleic Acids Res. 2006;34(21):e143). Isolated cells were picked in 1 µl of 1× PBS and transferred into 4.4 µl of lysing buffer containing 4 µl mTRAP™ Lysis Bu... |
27,820 | Pleurozium schreberi (moss) DNA extraction | 1 | dx.doi.org/10.17504/protocols.io.7ekhjcw | https://www.protocols.io/view/pleurozium-schreberi-moss-dna-extraction-7ekhjcw | Eric Pederson | TITLE: Pleurozium schreberi (moss) DNA extraction
AUTHORS: Eric Pederson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is a detailed protocol concerning the DNA extraction from the genome paper titled "</span><span style = "font-weight:bold;">Genome Sequencing of</span><span style = "fo... | ["[Tissue destruction]\nEnsure that the sterile bench is clean off and all the lab plastic and equipment is sprayed and placed in the sterile bench for at least 15 minutes.", "[Tissue destruction]\nGrind the tissue using mortar and pestle in the presence of liquid nitrogen until finely ground. Transfer frozen ground ti... |
78,347 | QIAGEN RNeasy Plant RNA Extraction Protocol (Modified) | 4 | null | https://www.protocols.io/view/qiagen-rneasy-plant-rna-extraction-protocol-modifi-cqrjvv4n | Steven J Burgess | TITLE: QIAGEN RNeasy Plant RNA Extraction Protocol (Modified)
AUTHORS: Steven J Burgess
[DESCRIPTION]
This is a protocol for extraction of RNA from plant leaf tissue using a QIAGEN RNeasy Plant Mini Kit. The procedure largely follows the manufacturer's instructions but there are a few minor tweaks we introduced which ... | ["[Loading samples] Add 450 µL Buffer RLT to a maximum of 100 mg tissue powder. Vortex immediately until the powder is re-suspended.", "[Loading samples] Spin samples 12000 x g, 1 min to pellet any residual debris.", "[Loading samples] Transfer the lysate to a QIAshredder spin column (lilac) placed in a 2 mL collection... |
101,954 | Human RA-gastruloid induction from pluripotent stem cells | 0 | dx.doi.org/10.17504/protocols.io.261ge5epog47/v1 | https://www.protocols.io/view/human-ra-gastruloid-induction-from-pluripotent-ste-dfta3nie | Nobuhiko Hamazaki | TITLE: Human RA-gastruloid induction from pluripotent stem cells
AUTHORS: Nobuhiko Hamazaki
[DESCRIPTION]
Gastruloids are a powerful in vitro model of early human development. However, although elongated and composed of all three germ layers, human gastruloids do not morphologically resemble post-implantation human em... | ["[Day0, Passage human PSCs onto vitronectin-coated NutriStem] Coat wells with Vitronectin and incubate them in 37C for at least 15 min", "[Day0, Passage human PSCs onto vitronectin-coated NutriStem] Dissociate pre-treated ESCs", "[Day0, Passage human PSCs onto vitronectin-coated NutriStem] Aspirate media from wells an... |
26,552 | UC Davis - Lipoprotein analysis by LTRS | null | dx.doi.org/10.17504/protocols.io.56yg9fw | null | Thomas Huser | TITLE: UC Davis - Lipoprotein analysis by LTRS
AUTHORS: Thomas Huser
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Raman spectroscopy analyzes non-elastically scattered light from chemical bonds (Fig. 1). Photons fro... | ["Preparation of TGRL samples1. Blood is obtained from volunteers by venipuncture and collected in 10 ml vacutainer tubes filled with streptokinase (150 units/ml blood). 2. Whole blood is then spun at 4ºC for 10 minutes at 1750g, to remove cellular particulate. 3. Plasma is transferred to ultracentrifuge tubes (Beckm... |
null | null | null | dx.doi.org/10.17504/protocols.io.iemcbc6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Adipogenic differentiation was induced by using the StemPro adipogenesis differentiation kit (Invitrogen, Carlsbad, CA, USA). WJCMSCs were grown in the adipose-inducing medium for 3 weeks. For Oil Red O staining, after induction, cells were fixed with 10% formalin for at leas... | [] |
78,360 | Study Schedule (Part 6 of "Effects of Online Exercise Intervention on Physical and Mental Conditions in Young Adults with Chronic Neck Pain") | 1 | dx.doi.org/10.17504/protocols.io.yxmvm2b7og3p/v1 | https://www.protocols.io/view/study-schedule-part-6-of-34-effects-of-online-exer-cqryvv7w | Yiting Lin | TITLE: Study Schedule (Part 6 of "Effects of Online Exercise Intervention on Physical and Mental Conditions in Young Adults with Chronic Neck Pain")
AUTHORS: Yiting Lin
[DESCRIPTION]
This is Part 6 of "Effects of Online Exercise Intervention on Physical and Mental Conditions in Young Adults with Chronic Neck P... | [] |
78,088 | Extraction of Cyanobacterial Slime from Community Samples and Subsequent Analysis via GC-MS | 4 | dx.doi.org/10.17504/protocols.io.5qpvorw8dv4o/v1 | https://www.protocols.io/view/extraction-of-cyanobacterial-slime-from-community-cqhgvt3w | Kelsey Cremin, Jerko Rosko, Sarah J.N. Duxbury, Mary Coates, Lijiang Song, Orkun Soyer | TITLE: Extraction of Cyanobacterial Slime from Community Samples and Subsequent Analysis via GC-MS
AUTHORS: Kelsey Cremin, Jerko Rosko, Sarah J.N. Duxbury, Mary Coates, Lijiang Song, Orkun Soyer
[DESCRIPTION]
This protocol details the extraction of cyanobacterial slime from community samples and subsequent analysis vi... | ["[PART A: Slime/EPS extraction] Use mature cultures (at least 35 days old, grown in BG11+ with full vitamin mix – see Table 1) and record culture origin and date of initiation. \n \n\nTable 1. Full vitamin mix, prepared as a one thousand times concentrated stock, as referenced in Duxbury et al. (2023)1\n Vitamins ... |
38,196 | Wastewater Concentration by Adsorption and Direct Extraction for SARS-CoV-2 RNA Detection and Quantification using RT-ddPCR | 1 | dx.doi.org/10.17504/protocols.io.bhiuj4ew | https://www.protocols.io/view/wastewater-concentration-by-adsorption-and-direct-bhiuj4ew | Aaron Bivins, Warish Ahmed, Devin North, Kyle Bibby | TITLE: Wastewater Concentration by Adsorption and Direct Extraction for SARS-CoV-2 RNA Detection and Quantification using RT-ddPCR
AUTHORS: Aaron Bivins, Warish Ahmed, Devin North, Kyle Bibby
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The following protocol describes the GERM Lab workflow... | ["[Sample Collection]\nCollect a sample of primary influent or raw wastewater (grab sample or composite) in a sterile sample collection bottle. Maintain the sample at during transport to the lab.\n1 L\n4 °C", "[Sample Collection]\nUpon sample receipt at the laboratory spike of resuspended INFORCE 3 into the wastew... |
null | null | null | dx.doi.org/10.17504/protocols.io.ek8bczw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Stellaris RNA FISH protocol to simultaneously label adherent cells with IF and RNA FISH.
[BEFORE_START]
<p align="LEFT">Reagents and Equipment</p>
<p align="LEFT">Reagents and Consumables:</p>
<p align="LEFT">a) TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)</p>
<p align="LEFT">... | [] |
30,166 | iPSC Cell Culture – Maintenance and Expansion | null | dx.doi.org/10.17504/protocols.io.9pwh5pe | null | Jacob Marsh, Celeste Karch | TITLE: iPSC Cell Culture – Maintenance and Expansion
AUTHORS: Jacob Marsh, Celeste Karch
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is about Maintenance and Expansion of induced pluripotent stem cells.</div></div>
[STEPS]
?. [Matrigel Coating]
To resuspend, thaw aliquot .
on ice
... | ["[Matrigel Coating]\nTo resuspend, thaw aliquot .\non ice", "[Matrigel Coating]\nAdd cold DMEM/F12.\n12.5 ml", "[Matrigel Coating]\nPipette up and down twice.", "[Matrigel Coating]\nAdd of Matrigel per well of 6 well plate.\n1 ml", "[Matrigel Coating]\nStore diluted Matrigel at .\n4 °C", "[Thawing iPSC]\nPrior to th... |
null | null | null | dx.doi.org/10.17504/protocols.io.sfvebn6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.c7zzp5 | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<strong>NOTE: We observe maximal growth with open ocean seawater (i.e. Sargasso seawater), though we can routinely grow <em>Prochlorococcus</em> in some coastal seawater (from Cape Cod, MA) as well.<br /><br />Table 1.</strong> Nutrient additions to filtered, autoclaved seawater ... | [] |
62,365 | Keto Start ACV Gummies | 3 | dx.doi.org/10.17504/protocols.io.3byl4bxqrvo5/v1 | https://www.protocols.io/view/keto-start-acv-gummies-b855ry86 | H Douglas Morris | TITLE: Keto Start ACV Gummies
AUTHORS: H Douglas Morris
[DESCRIPTION]
They have stand pat reply letters on health care reform that they send to all constituents who write in regarding health care matters.
[STEPS] | [] |
103,677 | Human Fixed Neuronal Nucleus Isolation for Single-Nucleus Transcriptomic Profiling (10x Genomics) | 0 | dx.doi.org/10.17504/protocols.io.8epv5r3w4g1b/v1 | https://www.protocols.io/view/human-fixed-neuronal-nucleus-isolation-for-single-dhg533y6 | Satoshi Ishishita, Katherin Gabriel, Seph Palomino, Allan-Hermann Pool | TITLE: Human Fixed Neuronal Nucleus Isolation for Single-Nucleus Transcriptomic Profiling (10x Genomics)
AUTHORS: Satoshi Ishishita, Katherin Gabriel, Seph Palomino, Allan-Hermann Pool
[DESCRIPTION]
Protocol for generating suspensions of fixed neuronal nuclei (NeuN+) from human central nervous system tissue for single... | ["[Equipment and Reagents] Equipment\nKimble Dounce Kontes tissue-grinder set (DWK 885300-0000)\n50 ml Oakridge tubes (#0556214D) / can replace with 50 mL Falcon tube\n15 mL Falcon tubes (Fisher #352097)\n50 mL Falcon tubes (Fisher #352070)\n1.5mL LoBind Eppendorf Tubes\n70-micron Corning Cell Strainer (#431751)\nFire ... |
43,629 | Collecting the Red Turpentine Beetle | 3 | dx.doi.org/10.17504/protocols.io.bnummeu6 | https://www.protocols.io/view/collecting-the-red-turpentine-beetle-bnummeu6 | Caroline Storer, Jiri Hulcr | TITLE: Collecting the Red Turpentine Beetle
AUTHORS: Caroline Storer, Jiri Hulcr
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to collect and preserve the red turpentine beetle (RTB), Dendroctonus valens.</div><div class = "text-block"><span>This protocol is part of the... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.erhbd36 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
61,372 | Measurement of OD to CFU ratio in bacteria | 4 | null | https://www.protocols.io/view/measurement-of-od-to-cfu-ratio-in-bacteria-b764rrgw | Gabriel Madirolas, Alid Al-Asmar, Alfonso Pérez Escudero | TITLE: Measurement of OD to CFU ratio in bacteria
AUTHORS: Gabriel Madirolas, Alid Al-Asmar, Alfonso Pérez Escudero
[DESCRIPTION]
A protocol for estimating CFU (colony forming units) to OD (optical density) for many bacterial species at once.
[STEPS]
1. Two days before the experiments: pick one or two colonies... | ["Two days before the experiments: pick one or two colonies from a plate and put them in culture in 5 mL of LB, in a 50mL Falcon. Incubate for 24 hours*.\n\n* Shaker 300 rpm, temperature 22,5°C. The tubes are kept closed.", "The day before: transfer 1µL from your tube to a new culture tube*, using a sterile inoculating... |
86,240 | Sanger Tree of Life Sample Homogenisation: PowerMash | 4 | dx.doi.org/10.17504/protocols.io.5qpvo3r19v4o/v1 | https://www.protocols.io/view/sanger-tree-of-life-sample-homogenisation-powermas-cyf8xtrw | Amy Denton, graeme oatley, Clare Cornwell, Michael Quail, Caroline Howard | TITLE: Sanger Tree of Life Sample Homogenisation: PowerMash
AUTHORS: Amy Denton, graeme oatley, Clare Cornwell, Michael Quail, Caroline Howard
[DESCRIPTION]
This protocol describes the homogenisation of tissue samples for DNA and/or RNA extraction intended for long read sequencing or RNA-Seq, using the Diagnocine Powe... | ["[Laboratory protocol] Transfer the sample into the sterile 1.5 mL BioMasher II tube and add the lysis buffer required for the downstream protocol.", "[Laboratory protocol] Attach the sterile BioMasher pestle to the PowerMasher II tissue disruptor, avoiding contact with the end of the pestle that will contact the samp... |
43,757 | Preparation of cigarette smoke extract (CSE) | 4 | dx.doi.org/10.17504/protocols.io.bnymmfu6 | https://www.protocols.io/view/preparation-of-cigarette-smoke-extract-cse-bnymmfu6 | Yoshiyuki Matsuo, Kiichi Hirota | TITLE: Preparation of cigarette smoke extract (CSE)
AUTHORS: Yoshiyuki Matsuo, Kiichi Hirota
[STEPS]
?. [Notes]
All procedures should be done in a fume hood to minimize exposure of the experimenter and laboratory environment to the cigarette smoke.The CSE should be prepared immediately before use.
?. [Materials]
1000 ... | ["[Notes]\nAll procedures should be done in a fume hood to minimize exposure of the experimenter and laboratory environment to the cigarette smoke.The CSE should be prepared immediately before use.", "[Materials]\n1000 µl pipette tipSilicone tubing (Tigers Polymer, SR-1554, inner diameter: 3 mm, outer diameter: 5 mm, l... |
null | null | null | dx.doi.org/10.17504/protocols.io.s22egge | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
44,320 | 3' Linker Ligate cDNA (On-Bead), and Cleanup | 4 | dx.doi.org/10.17504/protocols.io.bph8mj9w | https://www.protocols.io/view/3-39-linker-ligate-cdna-on-bead-and-cleanup-bph8mj9w | Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo | TITLE: 3' Linker Ligate cDNA (On-Bead), and Cleanup
AUTHORS: Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Profiling of RNA binding protein targets in vivo ... | ["[Anneal Linker]\nAdd (see Note 3).\n[InvRand3Tr3 adapter ]", "[Anneal Linker]\nAdd .\n[100% DMSO]", "[Anneal Linker]\nHeat at , , place immediately for >.\n75 °C\non ice", "[Prepare Ligation Master Mix on Ice; 12.8 μL Per Sample]\nPrepare Ligation Master Mix on Ice; 12.8 μL Per Sample: AB110× RNA Ligase Buffer (wi... |
85,586 | Human ovarian tissue procurement and processing for research | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdk29lmk/v1 | https://www.protocols.click/view/human-ovarian-tissue-procurement-and-processing-fo-cxtsxnne | hannah.anvari, asma.giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan francesca.duncan | TITLE: Human ovarian tissue procurement and processing for research
AUTHORS: hannah.anvari, asma.giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan francesca.duncan
[DESCRIPTION]
Purpose: This protocol is intended for use in the collection and storage of human ovarian tissue n a research setting. The protocol... | ["[Collection of ovarian tissue for research (in pathology)] Ovaries are evaluated grossly per standard protocol in Northwestern Medicine Pathology. Ovaries are measured, external surface examined and evaluated for neoplasia.", "[Collection of ovarian tissue for research (in pathology)] Section ovaries in 3-5 mm sectio... |
59,261 | Modified NEBNext® VarSkip Short SARS-CoV-2 Library Prep Kit for Illumina Platforms - adapted for wastewater samples | 1 | dx.doi.org/10.17504/protocols.io.b545q8y6 | https://www.protocols.io/view/modified-nebnext-varskip-short-sars-cov-2-library-b545q8y6 | Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim | TITLE: Modified NEBNext® VarSkip Short SARS-CoV-2 Library Prep Kit for Illumina Platforms - adapted for wastewater samples
AUTHORS: Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim
[DESCRIPTION]
PURPOSE:
This method was developed at the FDA’s Center for Food Safety and Applied Nu... | ["[cDNA Synthesis]", "[cDNA Synthesis] Gently mix and spin down the LunaScript RT SuperMix reagent. Prepare the cDNA synthesis reaction as described below:\n \n\nFor no template controls, mix the following components:", "[cDNA Synthesis] Flick the tubes or pipet up and down 10 times to mix followed by a quick spin.", "... |
31,029 | Short term effect of Aldicarb on C.elegans | null | dx.doi.org/10.17504/protocols.io.baivice6 | null | Priota Islam | TITLE: Short term effect of Aldicarb on C.elegans
AUTHORS: Priota Islam
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;">Aldicarb is a cholinesterase inhibitor which prevents the breakdown of acetylcholine in th... | ["Bleach synchronize the worms on a Friday", "Following Monday refeed the arrested L1s on OP50 seeded plates, make 2plates per strain keeping one plate at 20C and the other at 25C", "The day before tracking (Wednesday) add 35ul of 2.5mM AK to 24 imaging plates", "Also, seed those drugs treated 24 imaging plates and add... |
27,137 | VirION 2 | 2 | null | https://www.protocols.io/view/virion-2-6q9hdz6 | Marie Burris, Ben Temperton, Natalie Solonenko | TITLE: VirION 2
AUTHORS: Marie Burris, Ben Temperton, Natalie Solonenko
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This collection contains protocols for the extraction and preparation of DNA for long-read work and library amplification of long reads from microbial or viral DNA.</div></div>
[S... | [] |
41,538 | One Hour Covid Test Protocol | 4 | dx.doi.org/10.17504/protocols.io.bktakwie | https://www.protocols.io/view/one-hour-covid-test-protocol-bktakwie | Sarah Ware, Ellen Jorgensen, Chris Monaco | TITLE: One Hour Covid Test Protocol
AUTHORS: Sarah Ware, Ellen Jorgensen, Chris Monaco
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a fast (one hour), very simple LAMP-based Covid-19 diagnostic test using readily-available components that is appropriate for point-of-care use and also can ... | ["[OPTIONAL pre-preparation of Master Mix]\nLAMP Master Mix preparation (OPTIONAL, supplied premixed in our kit)NOTE: Kits will have LAMP Master Mix already prepared with guanidine and primers in it in PCR tubes. We are leveraging the work of scientists at New England Biolabs and include the preparation because of our ... |
75,890 | Capturing and Processing Slaking Images With a Multi-Well Plate | 6 | null | https://www.protocols.click/view/capturing-and-processing-slaking-images-with-a-mul-cncsvawe | Claire Phillips, robert.meadows | TITLE: Capturing and Processing Slaking Images With a Multi-Well Plate
AUTHORS: Claire Phillips, robert.meadows
[DESCRIPTION]
This describes a process to measure soil wet aggregate stability through slaking, or rapid immersion it water. It uses a multi-well plate to process many aggregates at one time. Air dry, pea-si... | ["[Sample preparation] Establish how you will count the wells (across or down) and create a spreadsheet identifying the sample in each well. \nExample Document:", "[Sample preparation] The well plate we provided a 3D print file for has a notch in the upper left corner to identify the first column and row.", "[Sample pr... |
31,415 | Lentivirus Production | null | dx.doi.org/10.17504/protocols.io.bawxiffn | null | Addgene the Nonprofit Plasmid Repository | TITLE: Lentivirus Production
AUTHORS: Addgene the Nonprofit Plasmid Repository
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is for Lentivirus production. To see the full abstract and additional resources, visit the </span><a href="https://www.addgene.org/protocols/lentivirus-p... | ["[Seeding cells]\nSeed 293T packaging cells at 3.8×106 cells per plate in DMEM complete in 10 cm tissue culture plates.", "[Seeding cells]\nIncubate the cells at , 5% CO2 for ~.\n37 °C", "[Transfection]\nGently aspirate media, add fresh DMEM complete containing cloroquine diphosphate and incubate ~.\n10 ml\nFor of... |
58,995 | Photo-oxidation Using MiniSOG with EM Preparation of Transfected Culture Cells | 1 | dx.doi.org/10.17504/protocols.io.eq2lynj8pvx9/v1 | https://www.protocols.io/view/photo-oxidation-using-minisog-with-em-preparation-b5utq6wn | Mason Mackey, Xiaokun Shu, Varda Lev-Ram, Thomas J. Deerinck, Yingchuan Qi, Ericka B. Ramko, Michael W. Davidson, Yishi Jin, Mark H. Ellisman, Roger Y. Tsien | TITLE: Photo-oxidation Using MiniSOG with EM Preparation of Transfected Culture Cells
AUTHORS: Mason Mackey, Xiaokun Shu, Varda Lev-Ram, Thomas J. Deerinck, Yingchuan Qi, Ericka B. Ramko, Michael W. Davidson, Yishi Jin, Mark H. Ellisman, Roger Y. Tsien
[DESCRIPTION]
Abstract taken from Plos Biology Journal: A Gene... | ["Cells are grown on glass bottom MatTek culture dishes. (P35G-0-14-C, MatTek Corp)", "Transfected Cells are fixed with 37oC 2% glutaraldehyde in *0.1M sodium cacodylate buffer pH 7.4 with 2.0 mM CaCl2 for 5 minutes at room temp and then for 55 minutes on ice.", "Wash 5X with 0.1M sodium cacodylate buffer pH 7.4 with 2... |
59,727 | Optimizing a Protocol for Long-term Storage of Duckweed Clones as Turions: Organization of Spirodela polyrhiza accessions in the RDSC | 1 | null | https://www.protocols.io/view/optimizing-a-protocol-for-long-term-storage-of-duc-b6jprcmn | Buntora Pasaribu, Yi-Feng Chen, Eric Lam | TITLE: Optimizing a Protocol for Long-term Storage of Duckweed Clones as Turions: Organization of Spirodela polyrhiza accessions in the RDSC
AUTHORS: Buntora Pasaribu, Yi-Feng Chen, Eric Lam
[DESCRIPTION]
This protocol details about long-term storage of duckweed clones as turions. It contains protocols from the The In... | ["[Current protocol for clone maintenance in the RDSC] Transfer approximately 5–10 duckweed fronds to three plates.", "[Current protocol for clone maintenance in the RDSC] Use three types of plates comprising of 0.5X SH, 0.5X SH + Cef+ Suc 0.1%, or 0.5X SH +Suc 0.5% (Cef = cefatoxime; cf. Lam and Acosta, 2019).", "[Cur... |
48,138 | DNA extraction from fecal samples | 4 | dx.doi.org/10.17504/protocols.io.bs9inh4e | https://www.protocols.io/view/dna-extraction-from-fecal-samples-bs9inh4e | Yoshiyuki Matsuo | TITLE: DNA extraction from fecal samples
AUTHORS: Yoshiyuki Matsuo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">DNA extraction from fecal samples</div></div>
[STEPS]
?. [Materials]
Phosphate-buffered saline (PBS)EZ-beads (Promega, AMR76813M): 2 mL tube containing 0.2 mm ceramic (zirconium dioxid... | ["[Materials]\nPhosphate-buffered saline (PBS)EZ-beads (Promega, AMR76813M): 2 mL tube containing 0.2 mm ceramic (zirconium dioxide) spheres and one 5 mm zirconia beadMaxwell RSC Blood DNA Kit (Promega, AS1400)MN Bead Tube Holder (MACHEREY-NAGEL, 740469): Rubber-foam adapter for processing bead tubes with Vortex-Genie ... |
35,229 | Library Adapter Preparation for Dual-Index Double Stranded DNA Illumina Sequencing | 1 | dx.doi.org/10.17504/protocols.io.bem5jc86 | https://www.protocols.io/view/library-adapter-preparation-for-dual-index-double-bem5jc86 | Franziska Aron, Guido Brandt | TITLE: Library Adapter Preparation for Dual-Index Double Stranded DNA Illumina Sequencing
AUTHORS: Franziska Aron, Guido Brandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Adapter preparation protocol for doubble-stranded ancient DNA libraries for Illumina Next-Generation-Sequencing, base... | ["[Adapter Preparation (Library Preparation Room)]\nPrepare P5 adapter ()Use one tube of a 0.2 ml PCR strip to set up the P5 adapter mix. ABCD1ReagentStock concentrationFinal concentration1× Volume [µl]2Oligo Hybridization Buffer10 ×1 ×103IS1_adapter.P5500 µM200 µM404IS3_adapter.P5+P7500 µM200 µM405UV HPLC-water106Tot... |
107,222 | Hybrid selection protocol using 10x Single-Cell RNA-Seq assay library | 0 | dx.doi.org/10.17504/protocols.io.x54v924jzl3e/v1 | https://www.protocols.io/view/hybrid-selection-protocol-using-10x-single-cell-rn-dkxw4xpe | Xian Adiconis, Joshua Z Levin | TITLE: Hybrid selection protocol using 10x Single-Cell RNA-Seq assay library
AUTHORS: Xian Adiconis, Joshua Z Levin
[DESCRIPTION]
This protocol is for target enrichment of cDNA libraries generated with 10x Genomics single-cell RNA-seq assays. The protocol consists of parts of vendor provided protocols with minor modif... | ["[Sample preparation] Use 750 ng of cDNA library generated with the 10x Chromium single-cell RNA-seq assay.", "[Sample preparation] Add 1 µL each of TS-p5 and TS-p7 blocking oligos.", "[Sample preparation] If the total volume exceeds 3.4 µL, use a speed-vac and set the temperature at 30 °C to reduce the volume to the ... |
49,384 | Collection and Post-Surgical Excision of Human Kidney Tissue through the Cooperative Human Tissue Network | 1 | dx.doi.org/10.17504/protocols.io.buggnttw | https://www.protocols.io/view/collection-and-post-surgical-excision-of-human-kid-buggnttw | Maya Brewer, Jamie Allen, Carrie Romer, Elizabeth Neumann, Agnes Fogo, Raymond Harris, Danielle Gutierrez, Mark De Caestecker, Jeff Spraggins | TITLE: Collection and Post-Surgical Excision of Human Kidney Tissue through the Cooperative Human Tissue Network
AUTHORS: Maya Brewer, Jamie Allen, Carrie Romer, Elizabeth Neumann, Agnes Fogo, Raymond Harris, Danielle Gutierrez, Mark De Caestecker, Jeff Spraggins
[DESCRIPTION]
<div class = "text-blocks"><div class =... | ["Kidney tissues are selected based on patient and clinical metadata (e.g. age", "Receive excised kidney from full nephrectomy.", "Cut kidney in half along the longest plane and open like a book.", "Cut out a portion of the kidney (~80 x 60 x 4 mm) that is farthest away from the tumor location.", "Take a picture of the... |
87,217 | Qaigen RNEasy RNA extration protocol | 4 | dx.doi.org/10.17504/protocols.io.dm6gp337dvzp/v2 | https://www.protocols.io/view/qaigen-rneasy-rna-extration-protocol-czerx3d6 | Jessica Pardy, Michael Dan Siemon, Dilan Joseph, Justin Donovan, Richard Gibson, christopher.degroot, Amanda Hamilton | TITLE: Qaigen RNEasy RNA extration protocol
AUTHORS: Jessica Pardy, Michael Dan Siemon, Dilan Joseph, Justin Donovan, Richard Gibson, christopher.degroot, Amanda Hamilton
[DESCRIPTION]
This protocol was employed by Western University for RNA extraction of wastewater samples for wastewater-based epimelogy in London, On... | ["[Protocol modified from QIAamp® Viral RNA Mini Handbook] Thoroughly mix wastewater sample then aliquot 40 mL into 50 mL Falcon tube. Centrifuge at 12000 RPM for 90 min. Decant supernatant, assume 280 µl pellet.", "[Protocol modified from QIAamp® Viral RNA Mini Handbook] Pipet 1120 µl prepared Buffer AVL into Falcon t... |
64,069 | Mouse Perfusion Protocol | 1 | dx.doi.org/10.17504/protocols.io.ewov1nmo7gr2/v1 | https://www.protocols.io/view/mouse-perfusion-protocol-catdsei6 | Michael Lee | TITLE: Mouse Perfusion Protocol
AUTHORS: Michael Lee
[DESCRIPTION]
This protocol details the mouse perfusion procedure.
[STEPS]
SECTION: For perfusion with 1x K+-free PBS:
1. Place mouse in isoflurane chamber until unresponsive to toe pinch stimuli.
SECTION: For perfusion with 1x K+-free PBS:
2. Proceed to cut abdomi... | ["[For perfusion with 1x K+-free PBS:] Place mouse in isoflurane chamber until unresponsive to toe pinch stimuli.", "[For perfusion with 1x K+-free PBS:] Proceed to cut abdominal skin down both sides to expose the diaphragm.", "[For perfusion with 1x K+-free PBS:] Cut around diaphragm to expose the heart.", "[For perfu... |
50,465 | Collection of protocols for cell-free protein synthesis | 2 | dx.doi.org/10.17504/protocols.io.bvh9n396 | https://www.protocols.io/view/collection-of-protocols-for-cell-free-protein-synt-bvh9n396 | Weston Kightlinger | TITLE: Collection of protocols for cell-free protein synthesis
AUTHORS: Weston Kightlinger
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the collection of protocols for cell-free protein synthesis.</div><div class = "text-block"><span>Read more on cell-free protein synthesis ... | [] |
71,511 | Preparation of tissue for transmission electron microscopy ultrastructural analysis | 4 | dx.doi.org/10.17504/protocols.io.n92ldpz9nl5b/v1 | https://www.protocols.io/view/preparation-of-tissue-for-transmission-electron-mi-ch3xt8pn | Jillian C Danne, Simon A Crawford, Rachel Templin, Joan Clark, Viola Oorschot, Georg Ramm | TITLE: Preparation of tissue for transmission electron microscopy ultrastructural analysis
AUTHORS: Jillian C Danne, Simon A Crawford, Rachel Templin, Joan Clark, Viola Oorschot, Georg Ramm
[DESCRIPTION]
A transmission electron microscope (TEM) enables the magnification and visualisation of cell and tissue ultrastruc... | ["[Fixation] All fixation and processing steps must be performed in a fume hood wearing appropriate personal protective equipment (PPE). The Material Safety Data Sheet (MSDS) for each chemical must be read before commencing.\n\nDissect out the tissue of interest on a Teflon plate or dental wax sheet using fine forceps ... |
37,707 | UNC Chapel Hill Zebrafish Aquaculture Core (ZAC) Environmental Summary | 1 | dx.doi.org/10.17504/protocols.io.bg3jjykn | https://www.protocols.io/view/unc-chapel-hill-zebrafish-aquaculture-core-zac-env-bg3jjykn | Michelle Altemara | TITLE: UNC Chapel Hill Zebrafish Aquaculture Core (ZAC) Environmental Summary
AUTHORS: Michelle Altemara
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Zebrafish (Danio rerio) are a popular animal model used in a variety of research areas. As with all animal models, husbandry conditions, including ... | [] |
71,714 | Native Barcoding of amplicons (96 well plates) | 1 | null | https://www.protocols.io/view/native-barcoding-of-amplicons-96-well-plates-ciaauaae | Alex Shaw, Catherine Troman, Joyce Akello, Erika Bujaki, Javier Martin, Nick Grassly | TITLE: Native Barcoding of amplicons (96 well plates)
AUTHORS: Alex Shaw, Catherine Troman, Joyce Akello, Erika Bujaki, Javier Martin, Nick Grassly
[DESCRIPTION]
The following protocol is for the preparation of amplicons for sequencing using the Oxford Nanopore Native barcoding kit version 14 with 96 barcodes (SQK-NBD... | ["[Preparing input DNA] Quantify the amplified DNA with a Qubit Broad Range dsDNA kit.", "[Preparing input DNA] Transfer 200 fmol of DNA per sample into a clean 96-well plate and adjust volume to 12.5 μl with nuclease free water. Mix gently by pipetting.\n200 fmol = ~155ng VP1, ~584ng PanEV, ~974ng PanPV", "[Preparing... |
null | null | null | dx.doi.org/10.17504/protocols.io.gwxbxfn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p style="text-align: center;"><img style="display: block; margin-left: auto; margin-right: auto;" src="https://s3.amazonaws.com/pr-journal/hnnfjtn.png" width="100" height="90" data-src="https://s3.amazonaws.com/pr-journal/hnnfjtn.png" data-ofn="nehirarma-üst-Tr.png" /> </p>
<p... | [] |
60,018 | SAPAP3 Genotyping Protocol | 4 | dx.doi.org/10.17504/protocols.io.36wgq7d7kvk5/v1 | https://www.protocols.io/view/sapap3-genotyping-protocol-b6usrewe | kajsbr | TITLE: SAPAP3 Genotyping Protocol
AUTHORS: kajsbr
[DESCRIPTION]
Protocol for SAPAP3 KO Mouse Model Genotyping from Dr. Lisa Gunaydin's Lab, University of California, San Francisco
[STEPS]
1. Cut the tails.
To cut the tails and mark the animals, keep in mind to:
● cut as little amount of the tail as possible;
● alw... | ["Cut the tails. \nTo cut the tails and mark the animals, keep in mind to:\n● cut as little amount of the tail as possible;\n● always clean the scissors properly in between animals (EtOH and bleach);\n● tattoo the animals at the same time as cutting the tails;\n● use iso if needed.", "Digest the tails.\nIncubate the ta... |
52,600 | Processamento de actígrafos pré-coleta - ActTrust | 5 | dx.doi.org/10.17504/protocols.io.bxkypkxw | https://www.protocols.io/view/processamento-de-act-grafos-pr-coleta-acttrust-bxkypkxw | Daniel Vartanian | TITLE: Processamento de actígrafos pré-coleta - ActTrust
AUTHORS: Daniel Vartanian
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Este protocolo faz parte do processo de coleta de dados actigráficos do Grupo Interdisciplinar de Pesquisa em Sono (GIPSO). Ele foi desenhado para os actígrafos pr... | ["[Kanban]\nAdicione o cartão da tarefa no quadro de trabalho do seu projeto (somente para projetos que usam Kanban)\nAbra o quadro de trabalho de seu projeto e adicione um cartão chamado Realizar o processamento de actígrafos pré-coleta na lista Em andamento.No cartão, adicione uma etiqueta chamada Processamento e os ... |
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