id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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35,712 | Opentrons COVID-19 testing: Stations A & B, BP Genomics kit, 24 samples | null | dx.doi.org/10.17504/protocols.io.be48jgzw | https://www.protocols.io/view/opentrons-covid-19-testing-stations-a-amp-b-bp-gen-be48jgzw | Max Marrone | TITLE: Opentrons COVID-19 testing: Stations A & B, BP Genomics kit, 24 samples
AUTHORS: Max Marrone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Opentrons and the Open Medicine Institute are developing an automated high-throughput COVID-19 testing protocol to submit to the FDA for an Emergency Us... | ["[Station A: Initial OT-2 setup]\nClean the Station A OT-2.", "[Station A: Initial OT-2 setup]\nStart pre-cooling the Temperature Module to . This is used to actively cool the internal extraction control RNA that will be added to each sample.\n4 °C", "[Station A: Initial OT-2 setup]\nPlace the following labware on the... |
81,702 | Mobile technologies as an implementation strategy in Primary Health Care: a scoping review | 1 | dx.doi.org/10.17504/protocols.io.ewov1op1plr2/v1 | https://www.protocols.io/view/mobile-technologies-as-an-implementation-strategy-ct2ewqbe | Rafael Aiello Bomfim, Jean Ribeiro Leite, Giovana Soares Buzinaro, Hazelelponi Cerqueira Leite, Jhenyffer Andrade Viana Cabral | TITLE: Mobile technologies as an implementation strategy in Primary Health Care: a scoping review
AUTHORS: Rafael Aiello Bomfim, Jean Ribeiro Leite, Giovana Soares Buzinaro, Hazelelponi Cerqueira Leite, Jhenyffer Andrade Viana Cabral
[DESCRIPTION]
Mobile health or m-health is defined as delivering health using a devic... | ["Mobile technologies as an implementation strategy in Primary Health Care: a scoping review"] |
13,076 | HMW DNA extraction for diatoms | 1 | dx.doi.org/10.17504/protocols.io.qzudx6w | https://www.protocols.io/view/hmw-dna-extraction-for-diatoms-qzudx6w | Raffaela Abbriano | TITLE: HMW DNA extraction for diatoms
AUTHORS: Raffaela Abbriano
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for genomic DNA extraction from diatom cell culture without use of phenol/chloroform. Resulting DNA fragments were suitable for nanopore sequencing.</div></div>
[STEPS]
?. [Har... | ["[Harvest cells]\nHarvest approximately 7 x 107 - 2 x 108 cells from mid-exponential phase by centrifugation (5000xg, 3 minutes)Wash pelleted cells once with 1X PBS (5000xg, 3 minutes) and remove the supernatant", "[Cell lysis and RNAse treatment]\nResuspend cells in 600 L Nuclei Lysis Solution (Promega #A7941)Freeze ... |
31,355 | Is bad news on TV tickers good news? The effects of voiceover and visual elements in a video on viewers’ assessment | null | dx.doi.org/10.17504/protocols.io.bau3ieyn | https://www.protocols.io/view/is-bad-news-on-tv-tickers-good-news-the-effects-of-bau3ieyn | Konrad Maj | TITLE: Is bad news on TV tickers good news? The effects of voiceover and visual elements in a video on viewers’ assessment
AUTHORS: Konrad Maj
[STEPS]
?. Designing base video material.Designing video materiał by professional journalists (2.5 min.) witn narration (voiceover). The narrative layer of the film concerned t... | ["Designing base video material.Designing video materiał by professional journalists (2.5 min.) witn narration (voiceover). The narrative layer of the film concerned the fictional politician, who was running for mayor in a local election. The logo of a fictional TV station (“Regionalna TV”) in the upper right corner wa... |
21,633 | LIBS Mapping of Mg/Ca ratios in marine mollusc shells | null | dx.doi.org/10.17504/protocols.io.zc9f2z6 | null | Niklas Hausmann, Amy Prendergast, Andreas Lemonis, Jana Zech, Patrick Roberts, Panagiotis Siozos, Demetrios Anglos | TITLE: LIBS Mapping of Mg/Ca ratios in marine mollusc shells
AUTHORS: Niklas Hausmann, Amy Prendergast, Andreas Lemonis, Jana Zech, Patrick Roberts, Panagiotis Siozos, Demetrios Anglos
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span> E</span><span style = "font-weight:bold;">lemental analysis ... | ["[Sample preparation]\nSection shells at the hinge and along the direction of growth. If necessary remove non-hinge parts of the section to reduce the overall size.Select the 'better' side of the two sections and clean off with and Prepare a rudimentary holder ('Vesselheim') using crumpled up aluminium foil ( and c... |
51,226 | Phage Display Library Prep Method | 4 | null | https://www.protocols.io/view/phage-display-library-prep-method-bv92n98e | Sabrina A Mann, Lillian Khan, Sara Vazquez, Caleigh Mandel-Brehm, Joseph Derisi | TITLE: Phage Display Library Prep Method
AUTHORS: Sabrina A Mann, Lillian Khan, Sara Vazquez, Caleigh Mandel-Brehm, Joseph Derisi
[DESCRIPTION]
This protocol was designed to be used after the Scaled High Throughput Vacuum PhIP Protocol or the Scaled Moderate Throughput Multichannel PhIP Protocol. The immunoprecipi... | ["[Lysis] Dilute phage lysis product in water at a 1:4 ratio. Phage lysis should be used either immediately after IP or within 1 week of being stored at 4 °C.\n\nNOTE: we have done several tests and noticed that diluting the phage lysate 1:4 in water results in the most product at the end. Other dilutions ranging from ... |
25,827 | Morris USF Lab protocol DEMO | null | dx.doi.org/10.17504/protocols.io.5gbg3sn | null | Lauren Segers, Kendall Morris, Sarah Nuding | TITLE: Morris USF Lab protocol DEMO
AUTHORS: Lauren Segers, Kendall Morris, Sarah Nuding
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Surgical Protocol]
Methods were as previously described (Morris et al., 2010; Ott et al., 2012). Briefly, data were obtained from 13 adult cats (3.1–5.8 kg) of either se... | ["[Surgical Protocol]\nMethods were as previously described (Morris et al., 2010; Ott et al., 2012). Briefly, data were obtained from 13 adult cats (3.1–5.8 kg) of either sex that were part of a larger dataset that included recording the response of brainstem neurons during the protocols detailed below. In some animals... |
61,817 | GUV preparation | 1 | dx.doi.org/10.17504/protocols.io.3byl4b398vo5/v1 | https://www.protocols.io/view/guv-preparation-b8kzrux6 | Liv Jensen, Chunmei Chang | TITLE: GUV preparation
AUTHORS: Liv Jensen, Chunmei Chang
[DESCRIPTION]
GUV preparation for membrane tube assay application
[STEPS]
1. GUV Preparation
1.1. Clean the coverglass.
1.2. Coat cleaned coverslips with 100 μL 5% (w/w) polyvinyl alcohol (PVA) with a molecular weight of 145,000 (Millipore).
1.3. Place the... | ["GUV Preparation", "Clean the coverglass.", "Coat cleaned coverslips with 100 μL 5% (w/w) polyvinyl alcohol (PVA) with a molecular weight of 145,000 (Millipore).", "Place the coated coverslip in a heating incubator at 60 °C to dry the PVA film for 30 min.", "Spread a lipid mixture with a molar composition of 70% DOPC,... |
101,556 | Genome assembly (Nanopore and Illumina reads) | 0 | dx.doi.org/10.17504/protocols.io.kxygxywyol8j/v1 | https://www.protocols.io/view/genome-assembly-nanopore-and-illumina-reads-dfeu3jew | Rafael Rodrigues Ferrari, Thiago Mafra Batista | TITLE: Genome assembly (Nanopore and Illumina reads)
AUTHORS: Rafael Rodrigues Ferrari, Thiago Mafra Batista
[DESCRIPTION]
This protocol offers detailed, step-by-step instructions for students and researchers to assemble nuclear genomes using long reads generated by Nanopore technology. Before assembling the genome, w... | ["[SEQUENCING QUALITY CHECK] ****LongQC (https://github.com/yfukasawa/LongQC)****\n\n***Prepare a .pbs file to run the analysis remotely on Sagarana***", "[CROSS-SPECIES CONTAMINATION FILTERIN] ****Magic-BLAST (https://ncbi.github.io/magicblast/)****\n\n***Index the database***\n \n***ONT whole-genome sequencing***\n\n... |
41,805 | Slot/Dot Blot protocol. | 4 | dx.doi.org/10.17504/protocols.io.bk3mkyk6 | https://www.protocols.io/view/slot-dot-blot-protocol-bk3mkyk6 | beldenwj | TITLE: Slot/Dot Blot protocol.
AUTHORS: beldenwj
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a simple slot/dot blot protocol for detecting antigen on a membrane. Using the method outline here, one can achieve results in under 90 min because it eliminates the need for running a gel, tra... | ["[Add sample]\n1. Apply individual (or pooled) saliva or processed nasal swabs to the slot/dot blot apparatus making note of sample location.2. Turn on the vacuum source so the sample get drawn onto the membrane.3. Disassemble the slot/dot blot apparatus and place the membrane back in 100 % methanol.4. Air dry the mem... |
70,345 | Laboratory Protocols | 1 | dx.doi.org/10.17504/protocols.io.ewov1o84ylr2/v1 | https://www.protocols.io/view/laboratory-protocols-cgxhtxj6 | lelisaptawati, Widana Primaningtyas, Paramasari Dirgahayu, Yusup Subagio Sutanto, Brian Wasita, Betty Suryawati, Titik Nuryastuti, Ari Probandari | TITLE: Laboratory Protocols
AUTHORS: lelisaptawati, Widana Primaningtyas, Paramasari Dirgahayu, Yusup Subagio Sutanto, Brian Wasita, Betty Suryawati, Titik Nuryastuti, Ari Probandari
[DESCRIPTION]
Nontuberculous mycobacterial (NTM) were identified using matrix-assisted laser desorption-ionization time of flight mass ... | ["NTM species identification", "NTM colonies were taken from LJ media using a 1 mL loop and put into a 1.5 mL microtube containing 0.5 mm of glass beads and 500 mL of 70% ethanol.", "The vortex was carried out for 15 minutes and then incubated vertically at room temperature for 10 minutes.", "Vortex was carried out aga... |
null | null | null | dx.doi.org/10.17504/protocols.io.pssdnee | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
72,533 | Extraction and qPCR of Environmental Surveillance samples for the detection of Salmonella Typhi | 4 | dx.doi.org/10.17504/protocols.io.8epv5zrw4v1b/v2 | https://www.protocols.io/view/extraction-and-qpcr-of-environmental-surveillance-ci3vugn6 | Dilip Abraham, Nicola Elviss, Nicholas Feasey, Nick Grassly, Jacob John, Gagandeep Kang, John Scott Meschke, Venkata Raghava Mohan, Satheesh Nair, Jonathan Rigby, Rajan Srinivasan, Catherine Troman, Christopher Uzzell, Nicolette Zhou | TITLE: Extraction and qPCR of Environmental Surveillance samples for the detection of Salmonella Typhi
AUTHORS: Dilip Abraham, Nicola Elviss, Nicholas Feasey, Nick Grassly, Jacob John, Gagandeep Kang, John Scott Meschke, Venkata Raghava Mohan, Satheesh Nair, Jonathan Rigby, Rajan Srinivasan, Catherine Troman, Christoph... | ["[DNA Extraction] For extraction from Moore swabs, empty the contents of a PowerBead tube provided in the extraction kit into the PowerBead tube used for storing the filter.\n\nFor the pellet from membrane filtration, resuspend the pellet in 800µl CD1 from the first step of the DNA extraction protocol, then transfer t... |
36,345 | HuBMAP UF TMC - FACS Sorting of Live Cells for 10x scRNASeq | null | dx.doi.org/10.17504/protocols.io.bfqzjmx6 | https://www.protocols.io/view/hubmap-uf-tmc-facs-sorting-of-live-cells-for-10x-s-bfqzjmx6 | Maigan Brusko | TITLE: HuBMAP UF TMC - FACS Sorting of Live Cells for 10x scRNASeq
AUTHORS: Maigan Brusko
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This standard operating procedure (SOP) provides instructions for staining and sorting live cells. This SOP applies to cryopreserved cells that are stained with L... | ["[Thaw Cryopreserved Cells]\na. In a 15 mL conical tube, warm 10 mL of cDMEM in a bead bath set to 37 o Cb. Thaw the cells in the cryovials in a water bath set at 37 o Cc. Immediately, resuspend the cells in 10 ml of RT cDMEM i. First, add 1 mL of cDMEM to the cryovial ii. Then, transfer the cells from the cryov... |
12,443 | FISH Protocol: filter hybridization | 1 | dx.doi.org/10.17504/protocols.io.4r3l2wb3l1y9/v1 | https://www.protocols.io/view/fish-protocol-filter-hybridization-qd3ds8n | Eva Petrova, Roey Angel | TITLE: FISH Protocol: filter hybridization
AUTHORS: Eva Petrova, Roey Angel
[DESCRIPTION]
FISH PROTOCOL FOR HYBRIDIZATION ON FILTERS
Filtration of water samples and paraformaldehyde fixation of microbial cells on polycarbonate-(PC)-filters.
For whole cell fixation, microbial cells should be concentrated from water sam... | ["Sterilize filtration unit and other equipment.", "Moisten CN support filters and PC filters (shiny side of the PC-filter should face upwards) in sterile water.", "Place moistened CN support filters and PC filters (shiny side up) in the filtration unit.", "Check planarity of filtration unit and filters with a water-le... |
55,698 | nCoV-2019 sequencing protocol for illumina | 1 | null | https://www.protocols.io/view/ncov-2019-sequencing-protocol-for-illumina-b2msqc6e | Kentaro Itokawa, Tsuyoshi Sekizuka, Masanori Hashino, Rina Tanaka, Satsuki Eto, Risa Someno, Makoto Kuroda | TITLE: nCoV-2019 sequencing protocol for illumina
AUTHORS: Kentaro Itokawa, Tsuyoshi Sekizuka, Masanori Hashino, Rina Tanaka, Satsuki Eto, Risa Someno, Makoto Kuroda
[DESCRIPTION]
This protocol was originally folked from "ARTIC amplicon sequencing protocol for MinION for nCoV-2019" by Josh Quick to adapt for any illu... | ["[cDNA preparation] Mix the following components in an 0.2mL 8-strip tube or 96 well PCR plate;\n\nComponent Volume\n\nLunaScript RT SuperMix 1.25 µL \nTemplate RNA (purified) 5.0 µL\nTotal 6.2... |
61,656 | Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes | 1 | dx.doi.org/10.17504/protocols.io.b8fyrtpw | https://www.protocols.io/view/isolation-cryo-laser-scanning-confocal-microscope-b8fyrtpw | Prerana Gogoi, Momoko Shiozaki, Eric Gouaux | TITLE: Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes
AUTHORS: Prerana Gogoi, Momoko Shiozaki, Eric Gouaux
[DESCRIPTION]
Ionotropic glutamate receptors (iGluRs) at postsynaptic terminals mediate the majority of fast excitatory neurotransmission in r... | ["[Preparation of crude synaptosomes from vGLUT1-mVenus knock-in mouse] Remove the whole brains of three mice (14-23 weeks old), immediately after being euthanized, and rinse the brains in ice-cold PBS buffer pH 7.4.", "[Preparation of crude synaptosomes from vGLUT1-mVenus knock-in mouse] Add 10 mL of ice-cold homogeni... |
44,895 | A New Method for Quantitative Tandem Mass Spectrometry of Protein Ubiquitination Based on Peptide Isobaric Labeling | 4 | null | https://www.protocols.io/view/a-new-method-for-quantitative-tandem-mass-spectrom-bp37mqrn | 181830691 , G G, te | TITLE: A New Method for Quantitative Tandem Mass Spectrometry of Protein Ubiquitination Based on Peptide Isobaric Labeling
AUTHORS: 181830691 , G G, te
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protein ubiquitination is a common post-translational modification in organisms, and it participates... | [] |
51,464 | Protocol: The efficacy of the Epley maneuver for benign paroxysmal positional vertigo (BPPV) in primary care setting: a systematic review and meta-analysis | 1 | dx.doi.org/10.17504/protocols.io.bwhgpb3w | https://www.protocols.io/view/protocol-the-efficacy-of-the-epley-maneuver-for-be-bwhgpb3w | Yusuke Saishoji, Norio Yamamoto, Takashi Fujiwara, Hideki Mori, Shunsuke Taito | TITLE: Protocol: The efficacy of the Epley maneuver for benign paroxysmal positional vertigo (BPPV) in primary care setting: a systematic review and meta-analysis
AUTHORS: Yusuke Saishoji, Norio Yamamoto, Takashi Fujiwara, Hideki Mori, Shunsuke Taito
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">... | [] |
109,115 | Controls, imaging, and analysis of AAV-Zombie and SpECTr experiments | 0 | dx.doi.org/10.17504/protocols.io.n2bvjn72pgk5/v1 | https://www.protocols.io/view/controls-imaging-and-analysis-of-aav-zombie-and-sp-dns35egn | Gerard Michael Coughlin | TITLE: Controls, imaging, and analysis of AAV-Zombie and SpECTr experiments
AUTHORS: Gerard Michael Coughlin
[DESCRIPTION]
This protocol describes best practices for designing, imaging, and analyzing AAV-Zombie and SpECTr experiments.
For more information on these methods, see:
https://www.biorxiv.org/content/10.110... | ["[Controls for AAV-Zombie and SpECTr experiments] This section outlines best practices for designing AAV-Zombie and SpECTr experiments. We divide these into 'necessary' and 'recommended' controls. \n\nWe strongly recommend including 'necessary' controls in every AAV-Zombie experiment, and these should be performed in ... |
93,354 | Standard DAB Staining for Free-floating Fixed NHP Brain Tissue | 4 | null | https://www.protocols.io/view/standard-dab-staining-for-free-floating-fixed-nhp-c7eizjce | Andreea C. Bostan | TITLE: Standard DAB Staining for Free-floating Fixed NHP Brain Tissue
AUTHORS: Andreea C. Bostan
[DESCRIPTION]
This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex.
This protocol has been te... | ["[Part I (Day 1)] Bring tissue to Room temperature in buffer (e g., Phosphate buffered saline, PBS) on an orbital shaker (30 min).", "[Part I (Day 1)] Prepare Peroxide Solution (0.3 - 3 % H2O2) in dH2O.\nE.g., for 10 mL 0.3% H2O2 use:\n100 µL 30% H2O2 \n9900 µL dH2O", "[Part I (Day 1)] Prepare Blocking Serum Solution ... |
23,661 | Electroporation transformation of FITC-dextran into Oxyrrhis marina - an early branching dinoflagellate. | null | dx.doi.org/10.17504/protocols.io.3cmgiu6 | null | Nick Irwin, Elisabeth Hehenberger, Liz Cooney, Yoshihisa Hirakawa, Patrick Keeling | TITLE: Electroporation transformation of FITC-dextran into Oxyrrhis marina - an early branching dinoflagellate.
AUTHORS: Nick Irwin, Elisabeth Hehenberger, Liz Cooney, Yoshihisa Hirakawa, Patrick Keeling
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">INTRODUCTION... | ["[Culturing and cell collection]\nCultures were fed heat-killed E. coli and grown on a 16h/8h light/dark cycle at 20 degrees, cultures were grown in f/2 media.The O. marina strain we are using is CCMP604.Cultures were grown to a minimum density of 20,000 cells/mL", "[Resuspend cells in electroporation buffer]\nResuspe... |
25,067 | Plant material preparation and salt imposition for PlantScreen analysis | null | dx.doi.org/10.17504/protocols.io.4qjgvun | null | Magdalena Julkowska, Mariam Awlia | TITLE: Plant material preparation and salt imposition for PlantScreen analysis
AUTHORS: Magdalena Julkowska, Mariam Awlia
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Reproducible and efficient high-throughput phenotyping approaches, combined with advances in genome sequencing, are facilita... | [] |
83,830 | Creating Plate Layout in FIVTools | 1 | dx.doi.org/10.17504/protocols.io.261ged62yv47/v1 | https://www.protocols.io/view/creating-plate-layout-in-fivtools-cv4ww8xe | Jason Waligorski | TITLE: Creating Plate Layout in FIVTools
AUTHORS: Jason Waligorski
[DESCRIPTION]
Plate layout creator in FIVtools
[STEPS]
SECTION: Creating Plate Layout in FIVTools
1. Open FIVTools
SECTION: Creating Plate Layout in FIVTools
2. Click Layouts
SECTION: Creating Plate Layout in FIVTools
3. Update Exp Name field (h... | ["[Creating Plate Layout in FIVTools] Open FIVTools", "[Creating Plate Layout in FIVTools] Click Layouts", "[Creating Plate Layout in FIVTools] Update Exp Name field (highlighted in blue above) to the corresponding FIV experiment Number (make sure to keep “FIV” in the name)", "[Creating Plate Layout in FIVTools] Click ... |
78,178 | Efficacy of Simple Urine Test based Machine Learning Screening Software in predicting UTI in pregnancy | 5 | null | https://www.protocols.io/view/efficacy-of-simple-urine-test-based-machine-learni-cqkavuse | Rajamaheswari N, Ilangovan Veerappan, Chandrakala Maran, Harini Sivamani, M. Meena Mahalingam, A. Rajagopal | TITLE: Efficacy of Simple Urine Test based Machine Learning Screening Software in predicting UTI in pregnancy
AUTHORS: Rajamaheswari N, Ilangovan Veerappan, Chandrakala Maran, Harini Sivamani, M. Meena Mahalingam, A. Rajagopal
[DESCRIPTION]
Constant vigilance to recognize and treat Urinary Tract Infection(UTI)both asy... | ["[Efficacy of Simple Urine Test based Machine Learning Screening Software in predicting UTI in pregnancy] What is the scope of Machine Learning Screening Software (MLSS) for Obstetricians? \n It is a simple screening tool used to diagnose and monitor bacteriuria throughout pregnancy from the results of the Simple Urin... |
18,942 | Selective protection and labelling of arginine/lysine side chains in HBSs of proteins | null | dx.doi.org/10.17504/protocols.io.wq6fdze | null | Phuong Thao Bui, Quentin Nunes, Yong Li, David G.Fernig | TITLE: Selective protection and labelling of arginine/lysine side chains in HBSs of proteins
AUTHORS: Phuong Thao Bui, Quentin Nunes, Yong Li, David G.Fernig
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Interactions between heparan sulfate proteoglycans and the partner proteins regulate many aspe... | ["[Binding]\nAF-heparin beads (Tosoh Biosciences GmbH, Stuttgart, Germany; binding capacity of 4 mg antithrombin III/mL) A mini affinity column was made by placing a plastic air filter as a frit at the end of a P10 pipette tip (Star Lab Ltd., Milton Keynes, UK) and then packed with 20 μL AF-heparin beads.The mini-colum... |
37,422 | Tissue freezing in Cryostor solution + processing | 1 | dx.doi.org/10.17504/protocols.io.bgsnjwde | https://www.protocols.io/view/tissue-freezing-in-cryostor-solution-processing-bgsnjwde | Regina Hoo, Carmen Sancho, Roser Vento-Tormo | TITLE: Tissue freezing in Cryostor solution + processing
AUTHORS: Regina Hoo, Carmen Sancho, Roser Vento-Tormo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Tissue freezing in cryostor solution and recovery for tissue processing</div></div>
[STEPS]
?. Chill Cryostor solution (CS10) on ice (C2874... | ["Chill Cryostor solution (CS10) on ice (C2874-Sigma).", "Most tissue is collected in Hyperthermosol and kept on ice or at .\n4 °C", "Place tissue in petri dish, add enough ice cold RPMI/10% FBS to cover the tissue (depend on tissue size).", "Mince tissue with 2 scapels, just like what we would do for enzymatic diges... |
34,534 | Basic Protocol 2: Tagging a gene of interest with AID | null | dx.doi.org/10.17504/protocols.io.bdyei7te | null | Kizhakke Mattada Sathyan, Thomas G. Scott, Michael J. Guertin | TITLE: Basic Protocol 2: Tagging a gene of interest with AID
AUTHORS: Kizhakke Mattada Sathyan, Thomas G. Scott, Michael J. Guertin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The next step in developing the ARF-AID system is to tag the gene of interest with AID. The ARF-AID system requires full... | ["[Cloning the guide RNA into pSpCas9(BB)-2A-GFP (pX458) – Digestion of the vector with BbsI]\nDigest of pX458 with BbsI at BbsI reaction mix:NEB buffer 2.1 5.0 µl BbsI enzyme 1.0 µl 100X BSA 0.5 µl pX458 plasmid 3.0 µg Water to 50 µl\n3 µg\n37 °C\nThe ... |
62,544 | Uncaged Male Enhancement [IS LEGIT MIXING] The Real Reason Everyone Is Buying! | 3 | dx.doi.org/10.17504/protocols.io.kqdg3p7yel25/v1 | https://www.protocols.io/view/uncaged-male-enhancement-is-legit-mixing-the-real-b9bqr2mw | H Douglas Morris | TITLE: Uncaged Male Enhancement [IS LEGIT MIXING] The Real Reason Everyone Is Buying!
AUTHORS: H Douglas Morris
[DESCRIPTION]
Aggregate rating locales are great spots for data about items like this male improvement supplement.
[STEPS] | [] |
94,746 | Wastewater grab sample processing with Nanotrap® Microbiome A particles (40 ml) | 4 | dx.doi.org/10.17504/protocols.io.x54v9px34g3e/v1 | https://www.protocols.io/view/wastewater-grab-sample-processing-with-nanotrap-mi-c8r2zv8e | Dilip Abraham, Venkata Raghava Mohan, Nirmal Kumar | TITLE: Wastewater grab sample processing with Nanotrap® Microbiome A particles (40 ml)
AUTHORS: Dilip Abraham, Venkata Raghava Mohan, Nirmal Kumar
[DESCRIPTION]
This protocol is used to carry out processing of wastewater using Nanotrap‱ Microbiome A Particles (Ceres Nanosciences). Nanotrap‱particles are affinity-capt... | ["[Wastewater grab sample processing with Nanotrap® Microbiome A particles] Wastewater grab samples collected in 50 mL centrifuge tubes are transported to the laboratory in cold chain, maintaining a temperature of 4 °C .", "Once received, place the sample tubes on a tube rack and leave at Room temperature for 10 minu... |
94,786 | Extraction and LC-MS/MS analysis of four steroids from mouse plasma and bone marrow | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdrmzlmk/v1 | https://www.protocols.io/view/extraction-and-lc-ms-ms-analysis-of-four-steroids-c8tazwie | Andrea Lovdel, Scott G Denham, William P Cawthorn, Natalie ZM Homer | TITLE: Extraction and LC-MS/MS analysis of four steroids from mouse plasma and bone marrow
AUTHORS: Andrea Lovdel, Scott G Denham, William P Cawthorn, Natalie ZM Homer
[DESCRIPTION]
Steroid hormones, including progesterone, testosterone and corticosterone, play a critical role in
growth, development, reproductive fun... | ["[Bone Marrow Collection and Homogenisation] Prepare 0.5 mL Eppendorf microtubes for all bone marrow samples by removing the bottom of a 0.5 mL microtube (Eppendorf) with a razor blade", "[Bone Marrow Collection and Homogenisation] Batch sizes of 20-40 bone marrow samples are most manageable. Isolate bone marrow (BM) ... |
75,616 | (official) Sample Collection in Herbarium Collections - Protocol#0 | 1 | dx.doi.org/10.17504/protocols.io.5qpvorq3dv4o/v1 | https://www.protocols.click/view/official-sample-collection-in-herbarium-collectio-cm38u8rw | Ricardo M. Borges, Gabriela de Assis Ferreira, andrewmagno | TITLE: (official) Sample Collection in Herbarium Collections - Protocol#0
AUTHORS: Ricardo M. Borges, Gabriela de Assis Ferreira, andrewmagno
[DESCRIPTION]
This document outlines the protocol for sample collection from Herbarium specimens. The Herbarium houses a vast collection of plant specimens, managed by a... | ["[Preparation for collection] Organize every sample that will be used in each study into an EXCEL Sheet;\ninclude information and comments on every sample\nhave information about classification grouping on every sample\neach sample should also have the filename for their analytical data indexed (these can be added aft... |
9,363 | Purification of (Kai) proteins via size exclusion chromatography | null | dx.doi.org/10.17504/protocols.io.mdtc26n | null | Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann | TITLE: Purification of (Kai) proteins via size exclusion chromatography
AUTHORS: Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol can be used to further puri... | ["[preparation of buffer and solutions]\nPrepare 1 l of each: Degassed MiliQDegassed 20 % EtOHDegassed running buffer [20-50 mM Tris/HCl (pH8), 150 mM NaCl, 1-2 mM DTT, only for KaiC proteins: 5 mM MgCl2, 1 mM ATP]", "[set-up of your liquid chromatography system]\nConnect degassed MilliQ with pump A and pump B of your ... |
46,960 | Neurolucida 360: Detecting muscle fiber orientation | 1 | null | https://www.protocols.io/view/neurolucida-360-detecting-muscle-fiber-orientation-br4qm8vw | Maci Heal | TITLE: Neurolucida 360: Detecting muscle fiber orientation
AUTHORS: Maci Heal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Detecting muscle fiber orientation in 3D microscopy images using Neurolucida 360.</div></div>
[STEPS]
?. [Setup]
Launch SPARC-enabled Neurolucida 360.
?. [Using the Detect ... | ["[Setup]\nLaunch SPARC-enabled Neurolucida 360.", "[Using the Detect Orientation tool]\nWhen your image is loaded, close the 3D window. From the TRACE ribbon, select the Detect Orientation icon.", "[Using the Detect Orientation tool]", "[Using the Detect Orientation tool]\nNOTE: If you are unsure of the size of your ... |
55,983 | Primer Design | 5 | dx.doi.org/10.17504/protocols.io.n92ldz3znv5b/v1 | https://www.protocols.io/view/primer-design-b2wpqfdn | Dakota Betz, Dakota Betz | TITLE: Primer Design
AUTHORS: Dakota Betz, Dakota Betz
[DESCRIPTION]
Protocol for designing primers in Geneious.
[STEPS]
SECTION: Programs and Dependencies
1. Geneious
Version 11.1.5
SECTION: File preparation
2. You will an alignment of sequences in Geneious to design primers. Select the sequences you want to des... | ["[Programs and Dependencies] Geneious\nVersion 11.1.5", "[File preparation] You will an alignment of sequences in Geneious to design primers. Select the sequences you want to design primers for/align, then Align/Assemble > Multiple Align and then select the alignment software you prefer (usually Mafft, Geneious works ... |
88,743 | RAISINs (RNA-seq for Profiling Intact Nuclei with Ribosome-bound mRNA) Nuclei Isolation from Mouse CNS Tissue Protocol | 1 | dx.doi.org/10.17504/protocols.io.4r3l22n5pl1y/v1 | https://www.protocols.io/view/raisins-rna-seq-for-profiling-intact-nuclei-with-r-c2wfyfbn | Allen Institute | TITLE: RAISINs (RNA-seq for Profiling Intact Nuclei with Ribosome-bound mRNA) Nuclei Isolation from Mouse CNS Tissue Protocol
AUTHORS: Allen Institute
[DESCRIPTION]
This protocol is used to isolate nuclei from mouse CNS tissue.
[STEPS] | [] |
91,289 | Distribution and Morphology of Calcitonin Gene-Related Peptide (CGRP) Innervation in Flat Mounts of Whole Rat Atria and Ventricles | 4 | dx.doi.org/10.17504/protocols.io.3byl4qd38vo5/v1 | https://www.protocols.io/view/distribution-and-morphology-of-calcitonin-gene-rel-c5dzy276 | Jin Chen, Kohlton T Bendowski, Ariege Bizanti, Yuanyuan Zhang, Jichao Ma, Donald Hoover, David Gozal, Kalyanam Shivkumar, Zixi (Jack) Cheng | TITLE: Distribution and Morphology of Calcitonin Gene-Related Peptide (CGRP) Innervation in Flat Mounts of Whole Rat Atria and Ventricles
AUTHORS: Jin Chen, Kohlton T Bendowski, Ariege Bizanti, Yuanyuan Zhang, Jichao Ma, Donald Hoover, David Gozal, Kalyanam Shivkumar, Zixi (Jack) Cheng
[DESCRIPTION]
Calcitonin gene-re... | ["[Whole heart dissection and flat-mount preparation] Rats were first deeply anesthetized with isoflurane (5%; 5-10 minutes), with an oxygen flow rate of 1 liter per minute. When the animals were not responsive to the hind-toe pinch withdrawal reflex, the chest was opened and an injection of heparin (100 units) was mad... |
83,255 | Extraction and Measurement of Polyphosphate and Inorganic Phosphorus from Microalgae Samples | 1 | dx.doi.org/10.17504/protocols.io.q26g7pzdqgwz/v1 | https://www.protocols.io/view/extraction-and-measurement-of-polyphosphate-and-in-cvixw4fn | Ying-Yu Hu, Zoe V. Finkel | TITLE: Extraction and Measurement of Polyphosphate and Inorganic Phosphorus from Microalgae Samples
AUTHORS: Ying-Yu Hu, Zoe V. Finkel
[DESCRIPTION]
Polyphosphate and inorganic phosphate are extracted by hot Tris buffer. After centrifugation, 500 uL of extract is used for inorganic phosphate measurement, 200 uL of ext... | ["[Sample collection] Filter microalgae in liquid media onto GF/F or PC filters, using gentle vacuum pressure (130 mmHg).", "[Sample collection] Rinse sample with filtered saline (no nutrients)", "[Sample collection] Place sample filters in cryogenic vials", "[Sample collection] Filter same volume of the blank media (w... |
91,097 | S-3 SOIL STORAGE | 4 | dx.doi.org/10.17504/protocols.io.j8nlko241v5r/v1 | https://www.protocols.io/view/s-3-soil-storage-c47zyzp6 | REDI-NET Consortium | TITLE: S-3 SOIL STORAGE
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol describes soil storage.
[GUIDELINES]
OBJECTIVE
To outline steps for properly storing field-collected soil samples and nucleic acid samples purified from these soil.
SUMMARY/SCOPE
The overarching aim of the REDI-NET is to develop a col... | ["[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Cool 96-well microfuge tube racks on ice.", "[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Each collected soil sample will be transferred to a 50 mL falcon tube with a unique sample ID. Put falcon tubes into cryo/freezer box.", "[STORAGE PROCEDURE FOR UNTREATED SAMPLE] Label the bo... |
null | null | null | dx.doi.org/10.17504/protocols.io.nwpdfdn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p> To perform a comparative analysis of the physiological, biochemical and agronomical parameters of durum wheat cultivars (<em>Triticum durum</em> Desf.) under water-deficit conditions, five cultivars were grown under irrigated (control) and rainfall (stressed) conditions. Dif... | [] |
98,718 | High throughtput screening on Hutu 80 and NCI h716 GCaMP reporter cell line | 0 | dx.doi.org/10.17504/protocols.io.36wgqn463gk5/v1 | https://www.protocols.io/view/high-throughtput-screening-on-hutu-80-and-nci-h716-dcm62u9e | Chaima Ezzine | TITLE: High throughtput screening on Hutu 80 and NCI h716 GCaMP reporter cell line
AUTHORS: Chaima Ezzine
[DESCRIPTION]
The purpose of this document is to describe the procedures for screening samples on the Hutu-80 and NCI h 716 GCaMP reporter cell line and the quality controls to be carried out on this line prior to... | ["Pre-screening tests : \nBefore starting the screenings, the condition of the cells must be checked by different tests:\n A mycoplasma detection test\n Selection pressure\n A cell line specific activation test used the week before the screening", "Cell seeding (Under PSM (disinfected with biocidal and 70% eth... |
20,716 | UC Davis - Digestible Energy by Bomb Calorimetry | null | dx.doi.org/10.17504/protocols.io.ygkftuw | null | Trina Knotts | TITLE: UC Davis - Digestible Energy by Bomb Calorimetry
AUTHORS: Trina Knotts
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Changes in fecal energy loss can result in differences in net energy balance and feed effici... | ["Mice are singly housed in clean cages with fresh food and water and low bedding.", "Weigh mouse and “food-in” (grams of rodent diet provided at start of experiment).", "Save several pellets of rodent diet for analysis (e.g., for energy or macronutrient content)-Store at 4C in airtight container.", "At 24h, weigh mou... |
44,326 | Detection of Rare, Antigen-specific Human T cells with HLA Tetramers | 4 | null | https://www.protocols.io/view/detection-of-rare-antigen-specific-human-t-cells-w-bpiemkbe | Zaki Molvi | TITLE: Detection of Rare, Antigen-specific Human T cells with HLA Tetramers
AUTHORS: Zaki Molvi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>How I stain human T cells with tetramers following the guidelines from Sewell's group: </span><a href="https://pubmed.ncbi.nlm.nih.gov/26076649" style... | ["[Staining]\nHarvest cells of interest (PBMC, purified T cells, BMMC) either from culture or from frozen stock", "[Staining]\nWash cells in PBS and resuspend in 50nM Dasatinib in PBS. Choose a volume appropriate with your number of samples in the next step. You will need 50uL of sample per tetramer. Incubate 37C for 3... |
90,815 | CRISPR knock-in validation | 1 | dx.doi.org/10.17504/protocols.io.n2bvj3qb5lk5/v1 | https://www.protocols.io/view/crispr-knock-in-validation-c4w7yxhn | Leonardo A Parra-Rivas | TITLE: CRISPR knock-in validation
AUTHORS: Leonardo A Parra-Rivas
[DESCRIPTION]
CRISPR knock-in validation
[STEPS]
1.
Cultured hippocampal neurons tagged with oScarlet were prepared with Lucigen
QuickExtract DNA extraction solution (Biosearch Technologies, cat# QE09050).
Briefly, neurons were lysed by adding 50 u... | ["Cultured hippocampal neurons tagged with oScarlet were prepared with Lucigen\nQuickExtract DNA extraction solution (Biosearch Technologies, cat# QE09050).\nBriefly, neurons were lysed by adding 50 ul of QuickExtract solution to each\nsample.", "The samples were mixed by pipetting and incubated at 68 °C for 15 min\nfo... |
44,286 | Quantification by Droplet Digital PCR (ddPCR) | 4 | null | https://www.protocols.io/view/quantification-by-droplet-digital-pcr-ddpcr-bpg6mjze | Shuchen Feng, Adélaïde Roguet | TITLE: Quantification by Droplet Digital PCR (ddPCR)
AUTHORS: Shuchen Feng, Adélaïde Roguet
[STEPS]
?. When all reagents are thawed on ice, vortex Supermix, Reverse transcriptase and DTT throughly for 30 seconds. Vortex to mix primers and probes stocks.
?. Plate Set up: see sub-steps below.
This step requires to keep ... | ["When all reagents are thawed on ice, vortex Supermix, Reverse transcriptase and DTT throughly for 30 seconds. Vortex to mix primers and probes stocks.", "Plate Set up: see sub-steps below.\nThis step requires to keep the mixture cold/on ice.", "Droplet generation handling.\nIt is NOT required to keep the plate cold/o... |
null | null | null | dx.doi.org/10.17504/protocols.io.miyc4fw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>As climate change progresses, so does the risk from hurricanes, flooding, and other natural disasters. As sea level rises, tropical cyclones will pose a greater risk of extreme flooding and are likely to inflict the greatest damages on highly populated shorelines. A catastro... | [] |
33,183 | Cloning of standardized L0 parts into pUAP4 for Loop type IIS assembly | null | dx.doi.org/10.17504/protocols.io.bcm7iu9n | null | Eftychis Frangedakis, Susana Sauret-Gueto | TITLE: Cloning of standardized L0 parts into pUAP4 for Loop type IIS assembly
AUTHORS: Eftychis Frangedakis, Susana Sauret-Gueto
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for cloning of DNA L0 parts into pUAP4 using SapI type IIS assembly.</div><div class = "text-block"><span>A standa... | ["[Summary of primers and cloning into pUAP4]", "[PCR amplification of DNA part]\nDesign primers as described in step 1 to add the SapI recognition site, a partial BsaI recognition site, and the common syntax fusion site.", "[PCR amplification of DNA part]\nL0 parts are PCR amplified from the source DNA part (e.g. plas... |
57,986 | The Summary of Treatment Protocol in motivational psychotherapy | 1 | dx.doi.org/10.17504/protocols.io.b4vaqw2e | https://www.protocols.io/view/the-summary-of-treatment-protocol-in-motivational-b4vaqw2e | Hosein Sahebdel, Mohammad Tahan | TITLE: The Summary of Treatment Protocol in motivational psychotherapy
AUTHORS: Hosein Sahebdel, Mohammad Tahan
[DESCRIPTION]
Reviewing the history of theories associated with psychotherapy establishes that motivational psychotherapy (MPT) has been centered on environmental mind, lies, selfishness, and rich mind, wh... | ["Rapport> \tRapport, providing introductory acquaintance, having discussion about the purpose of the therapy, building trust.", "Describing> \tHelping clients to describe themselves by emphasizing incompatible aspects of their personality, this description includes all the characteristics of clients, including cogniti... |
8,691 | DNA quantification with PicoGreen | 1 | dx.doi.org/10.17504/protocols.io.4r3l2p84g1y9/v1 | https://www.protocols.io/view/dna-quantification-with-picogreen-kqtcvwn | Anna Székely | TITLE: DNA quantification with PicoGreen
AUTHORS: Anna Székely
[DESCRIPTION]
We use the Quant-iT™ PicoGreen® dsDNA kit for quantification of both extracted and amplified DNA.
The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. Free dye does not fluoresce, but upon binding to dsDNA it ex... | ["Prepare TE\n \n Calculate the total volume of 1xTE needed: \n200 µl per each technical replicate of your samples\n100 µl per standard\nTotal of 3-400 µl for the DNA standard dilution (depending on the assay you use, see step 2)\nMake enough to count for pipetting losses!!!\nE. g. for high range: 20 samples + 2* 4 sta... |
50,942 | LIS-001 INSPECT CLIA User Manual version 1.0 | 5 | null | https://www.protocols.io/view/lis-001-inspect-clia-user-manual-version-1-0-bvy6n7ze | Adam N Baer, Shashank Sathe | TITLE: LIS-001 INSPECT CLIA User Manual version 1.0
AUTHORS: Adam N Baer, Shashank Sathe
[DESCRIPTION]
Document Title: INSPECT CLIA User Manual
Document No. LIS-001
Document version: 1.0
User guide for the INSPECT (Instant Service Platform for Emergency COVIDTests) CLIA LIS application for use by EXCITE (Expedited C... | ["[Standard (Thermo) Workflow] Upload platemap file.", "[Standard (Thermo) Workflow] Pre-Scan Barcodes.", "[Standard (Thermo) Workflow] Select Pre-Scan Barcodes from the workflow steps listed in boxes:", "[Standard (Thermo) Workflow] Follow the instructions on the page to enter or scan in barcodes. Turn on the newline... |
21,055 | UC Davis - Metabolomics: Lipidomics analysis | null | dx.doi.org/10.17504/protocols.io.ys7fwhn | null | Oliver Fiehn | TITLE: UC Davis - Metabolomics: Lipidomics analysis
AUTHORS: Oliver Fiehn
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">Lipidomic analysis by UPLC-QTOF mass spectrometry</div></div>
[STEPS]
?. Pre-run procedures1.1 I... | ["Pre-run procedures1.1 Instrument tuning (Instrument in Tune mode) a. Use “Standard Tune” before each run of 300 sample batch. b. Use the “Tuning Solution” (see preparation of solutions below) for the instrument tuning. c. The mixture for the instrument tuning must be prepared fresh at the beginning of each 300 sampl... |
40,078 | Protein Extraction | 3 | dx.doi.org/10.17504/protocols.io.bjdnki5e | https://www.protocols.io/view/protein-extraction-bjdnki5e | avinash.kale | TITLE: Protein Extraction
AUTHORS: avinash.kale
[STEPS] | [] |
41,899 | Thermal cycling of the PCR | 4 | dx.doi.org/10.17504/protocols.io.bk6jkzcn | https://www.protocols.io/view/thermal-cycling-of-the-pcr-bk6jkzcn | philippe.bechtold | TITLE: Thermal cycling of the PCR
AUTHORS: philippe.bechtold
[STEPS]
?. Incubation:
[at 55°C]
?. Initial Denaturation
[at 92°C]
?. 45 cycles of the follwoing:Fluorescence is measured at the end of each 55°C period.
[at 55°C]
[95°C] | ["Incubation:\n[at 55°C]", "Initial Denaturation\n[at 92°C]", "45 cycles of the follwoing:Fluorescence is measured at the end of each 55°C period.\n[at 55°C]\n[95°C]"] |
53,685 | Analysis of protein structure using Molprobity | 1 | null | https://www.protocols.io/view/analysis-of-protein-structure-using-molprobity-bynvpve6 | Chris Berndsen | TITLE: Analysis of protein structure using Molprobity
AUTHORS: Chris Berndsen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Molprobity is a valuable collection of structural analysis tools for the validation or "correctness" of protein structures and homology models. This protocol walks through th... | ["This protocol describes submitting a .pdb file to the Molprobity server hosted at Duke University. Molprobity has now been incorporated into SWISS-Model results as well. The analysis aspects of either method are comparable and the analysis section can be applied to data from this latter instance. See the sections on... |
null | null | null | dx.doi.org/10.17504/protocols.io.fs9bnh6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p> </p>
<p><strong><u>Background Information</u></strong></p>
<ul>
<li>High throughput amplicon sequencing is an extremely sensitive technique. The lab environment is full of airborne bacterial and fungal cells as well as bacterial and fungal genomic DNA from cultures, and so e... | [] |
65,060 | Phage amplification and concentration | 4 | dx.doi.org/10.17504/protocols.io.yxmvmnb86g3p/v1 | https://www.protocols.io/view/phage-amplification-and-concentration-cbscsnaw | Adair Borges, Januka Athukoralage | TITLE: Phage amplification and concentration
AUTHORS: Adair Borges, Januka Athukoralage
[DESCRIPTION]
This protocol details methods to amplify bacteriophages T4 and SPO1, and concentrate using either PEG precipitation or filtration.
[STEPS]
SECTION: Phage amplification
2.
Grow a 3 mL overnight culture of phage ho... | ["[Phage amplification] Grow a 3 mL overnight culture of phage host at 37 °C with shaking. Escherichia coli strain B (ATCC Strain 11303) should be propagated in Lysogeny Broth (ATCC Medium 1065), and Bacillus subtilis strain 168M (ATCC Strain 27370) is propagated in Enriched Nutrient broth (ATCC Medium 265).", "[Phage ... |
88,520 | DNA Isolation (Gel Clean-up) | 4 | dx.doi.org/10.17504/protocols.io.81wgbxnk3lpk/v2 | https://www.protocols.io/view/dna-isolation-gel-clean-up-c2pgydjw | NUS iGEM | TITLE: DNA Isolation (Gel Clean-up)
AUTHORS: NUS iGEM
[DESCRIPTION]
2023 NUS-Singapore iGEM team followed this protocol to isolate the DNA fragments from the agarose gel after the gel electrophoresis.
[STEPS]
1. Prepare and label an Eppondoft tube.
2. Place the agarose gel onto the LED Transilluminator (blue light) ... | ["Prepare and label an Eppondoft tube.", "Place the agarose gel onto the LED Transilluminator (blue light) to observe the DNA band(s).", "Cut out the target DNA band from the agarose gel.", "Put the gel piece into the Eppendorf tube.", "Add 450 µL of into the Eppendorf tube.", "Heat the Eppendorf tube at 55 °C for 20... |
null | null | null | dx.doi.org/10.17504/protocols.io.dij4cm | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<strong>Primers<br /></strong>I use enzyme x to work with DNA sequences, it is installed on all the macs and has nice translation and reverse complement functions. Many of the same functions are available in a plasmid editor , which has Mac, Windows, and Linux versions. Choose a ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.nuvdew6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The 18S protocol detailed here is designed to amplify eukaryotes broadly with a focus on microbial eukaryotic lineages. The primers target the 18S SSU rRNA and are based on those of Amaral-Zettler et al. (2009). The constructs are designed to be used with the Illumina platfor... | [] |
105,893 | Publishing Generic Organ Scaffold as a SPARC Dataset | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8q72lmk/v2 | https://www.protocols.io/view/publishing-generic-organ-scaffold-as-a-sparc-datas-djnd4ma6 | Mabelle Lin, Hugh Sorby | TITLE: Publishing Generic Organ Scaffold as a SPARC Dataset
AUTHORS: Mabelle Lin, Hugh Sorby
[DESCRIPTION]
In this protocol, we will demonstrate how to prepare a generic organ scaffold for publication as a SPARC dataset using the Scaffold mapping tools. This protocol will ensure that a generic organ scaffold is made a... | ["Use the latest official version of the Scaffold mapping tools to generate your organ scaffold. Do not use a developer installation to generate a scaffold.", "Use the standard generic organ scaffold workflow to generate organ scaffold files. The workflow is installed by default with the mapping tools MAP Client instal... |
51,910 | Standard Protocol NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®) (NEB #E7650S/L) | 1 | dx.doi.org/10.17504/protocols.io.bwxepfje | https://www.protocols.io/view/standard-protocol-nebnext-artic-sars-cov-2-library-bwxepfje | Isabel Gautreau | TITLE: Standard Protocol NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®) (NEB #E7650S/L)
AUTHORS: Isabel Gautreau
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), NEB #E7650S/L 24/96 reactions. </d... | ["[cDNA Synthesis]\nGently mix and spin down the LunaScript RT SuperMix reagent. Prepare the cDNA synthesis reaction as described below:COMPONENT VOLUMERNA Sample8 µl(lilac) LunaScript RT SuperMix2 µlTotal Volume10 µlFor no template controls, mix the following components:COMPONENTVOLUME(white) Nuclease-free Water8 µl(l... |
42,069 | Mathematical models for understanding the genetic diversity of the new coronavirus: LaBECom's analytical protocols | 5 | dx.doi.org/10.17504/protocols.io.bmbvk2n6 | https://www.protocols.io/view/mathematical-models-for-understanding-the-genetic-bmbvk2n6 | Pierre Teodosio Felix, Robson da Silva Ramos, Dallynne Bárbara Ramos Venâncio, Cícero Batista do Nascimento Filho, Antônio João Paulino | TITLE: Mathematical models for understanding the genetic diversity of the new coronavirus: LaBECom's analytical protocols
AUTHORS: Pierre Teodosio Felix, Robson da Silva Ramos, Dallynne Bárbara Ramos Venâncio, Cícero Batista do Nascimento Filho, Antônio João Paulino
[DESCRIPTION]
<div class = "text-blocks"><di... | ["[Results]\nMolecular Variance Analysis (AMOVA) and Genetic Distance", "[WORK OVERVIEW]\nThe specific methodologies for paired FST estimators, Molecular Variance (AMOVA), Genetic Distance, mismatch, demographic and spatial expansion analyses, molecular diversity and evolutionary divergence time were obtained using 20,... |
78,126 | In situ Blimp1 detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues | 4 | dx.doi.org/10.17504/protocols.io.rm7vzbqqxvx1/v1 | https://www.protocols.io/view/in-situ-blimp1-detection-in-formalin-fixed-paraffi-cqinvude | Jayne E Wiarda, Crystal Loving | TITLE: In situ Blimp1 detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues
AUTHORS: Jayne E Wiarda, Crystal Loving
[DESCRIPTION]
An immunohistochemistry (IHC) staining protocol for in situ identification of porcine Blimp1
[BEFORE_START]
Starting specimens:
Starting samples = FFPE tissues cut to 4 micron ... | ["[Baking] Before starting the assay: \nPreheat a dry oven to 60℃ \nLoad slides for assay into vertical slide rack\n\nBaking\nBake slides 20 min 60℃\n\nWhile slides bake:\nPrepare 0.05% PBS-T (can store at RT up to 1 month)", "[Deparaffinizing & Rehydrating] Immediately before deparaffinizing:\nAdd ~200 mL xylenes ... |
23,962 | The protocol for the mannequin crossover trial entitled "Comparison of video and conventional laryngoscopes for simulated difficult emergency tracheal intubations in presence of liquids in the airway" | null | dx.doi.org/10.17504/protocols.io.3m2gk8e | null | Kei Suzuki, Shinji Kusunoki, Takuma Sadamori, Yuko Tanabe, Junji Itai, Nobuaki Shime | TITLE: The protocol for the mannequin crossover trial entitled "Comparison of video and conventional laryngoscopes for simulated difficult emergency tracheal intubations in presence of liquids in the airway"
AUTHORS: Kei Suzuki, Shinji Kusunoki, Takuma Sadamori, Yuko Tanabe, Junji Itai, Nobuaki Shime
[DESCRIPTION]
<di... | ["Ethics Approval: This study was approved by the Ethics committee of the Hiroshima University (E-115, E-609). Written consent to participate in the study was obtained from all EMT participants. Data were collected from EMTs who were qualified and certified to perform TIs with ML and AWS in patients with cardiac arrest... |
27,674 | MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection | null | dx.doi.org/10.17504/protocols.io.692hh8e | null | Kelsey Miller, Sam Li | TITLE: MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection
AUTHORS: Kelsey Miller, Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external ... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) polypropylene tube.Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells with... |
19,793 | Differentiation of dendritic cells from human monocytes | null | dx.doi.org/10.17504/protocols.io.xjrfkm6 | null | Girija Goyal | TITLE: Differentiation of dendritic cells from human monocytes
AUTHORS: Girija Goyal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the steps conventionally used to differentiate dendritic cells from human monocytes isolated from fresh whole blood or leukopaks. While these D... | ["[Plate monocytes]\nPlate monocytes in a 24 well plate with 1 million monocytes/well in 1 ml of RPMI+400ng/ml GM-CSF and 250ng/ml IL4.", "[First media change]\n2 days later (maximum 3 days), assess if there are any non-adherent, small cells floating around. These might be neutrophils or lymphocytes. If the number of t... |
50,156 | Background and Rationale (Part 1 of Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus) | 1 | dx.doi.org/10.17504/protocols.io.bu8knzuw | https://www.protocols.io/view/background-and-rationale-part-1-of-safety-and-effi-bu8knzuw | Stephen.Gitelman , Jeffrey A. Bluestone | TITLE: Background and Rationale (Part 1 of Safety and Efficacy of Imatinib for Preserving Beta-Cell Function in New-onset Type 1 Diabetes Mellitus)
AUTHORS: Stephen.Gitelman , Jeffrey A. Bluestone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is Part 1 of "Safety and Efficacy of Imatinib for... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kp8cvrw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>A protein B18R is encoded by Western Reserve (WR) strain of <em>Vaccinia virus</em>. B18R works as a decoy receptor for the type I interferons (IFNs), attaches to cell surfaces, binds IFNs without prominent species-specificity and prevents an establishment of the antiviral st... | [] |
51,728 | Changing antibiotic prescribing practices in outpatient primary care settings in China: study protocol for a health information system-based cluster-randomised crossover controlled trial | 1 | dx.doi.org/10.17504/protocols.io.bwrqpd5w | https://www.protocols.io/view/changing-antibiotic-prescribing-practices-in-outpa-bwrqpd5w | Anonymous | TITLE: Changing antibiotic prescribing practices in outpatient primary care settings in China: study protocol for a health information system-based cluster-randomised crossover controlled trial
AUTHORS: Anonymous
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Back... | ["[AI-based real-time warning pop-up windows of improper antibiotic use]\nBased on the HIS system of primary careinstitutions, the warning plug-in uses graph neural network technology to automatically access the prescription data in the background. It will compare each prescriptionwith the big data and DMNNmodeling res... |
83,501 | Protocolo Reto F1017B | 1 | dx.doi.org/10.17504/protocols.io.j8nlkob7wv5r/v1 | https://www.protocols.click/view/protocolo-reto-f1017b-cvsmw6c6 | Valentina Lugo Moreno | TITLE: Protocolo Reto F1017B
AUTHORS: Valentina Lugo Moreno
[DESCRIPTION]
Este archivo es con el propósito de explicar los pasos para llevar a cabo el experimento "motor lineal" del bloque F1017B, detallando los materiales, pasos para hacer el prototipo y como llevar a cabo el experimento.
[BEFORE_START]
Conseguir lo... | ["[Prototipo] Medir la tabla y los imanes, encontrando una relación entre ambos para darles la distancia necesaria (misma distancia entre cada imán y la madera 92mm x 30mm x 14 mm), realizar un boceto en la madera 400mm x 90mm x 20mm para tener fijo el lugar de cada material.", "[Prototipo] Con la cegueta hacer ranuras... |
41,416 | HolographicDiagnostics: Automated Virus Binding Assay | 1 | dx.doi.org/10.17504/protocols.io.bkpgkvjw | https://www.protocols.io/view/holographicdiagnostics-automated-virus-binding-ass-bkpgkvjw | Rushna Quddus, Kaitlynn Snyder, Lauren Altman, Laura Philips, David G. Grier, Andrew D. Hollingsworth, Kent Kirshenbaum | TITLE: HolographicDiagnostics: Automated Virus Binding Assay
AUTHORS: Rushna Quddus, Kaitlynn Snyder, Lauren Altman, Laura Philips, David G. Grier, Andrew D. Hollingsworth, Kent Kirshenbaum
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the steps required to perform a hologr... | ["[Sample Collection]\nSubject passively collects saliva for testing.\n[saliva]", "[Sample Preparation]\nHeat treat patient sample to inactivate virus.\n65 °C\nPatient samples must remain sealed until they are heat treated. BSL3 facilities are required to work with untreated samples. Heat-treated samples may be opened ... |
107,327 | data2 | 0 | dx.doi.org/10.17504/protocols.io.14egn6o6pl5d/v1 | https://www.protocols.io/view/data2-dk274yhn | ls_hust Li | TITLE: data2
AUTHORS: ls_hust Li
[DESCRIPTION]
Pulse signal fitting
[STEPS]
1. Extract the pulse signal portion that exceeds the threshold voltage
2. Fit using different functions
3. Extract AIC, BIC, R2 values for each pulse signal when fitted with different fitting functions | ["Extract the pulse signal portion that exceeds the threshold voltage", "Fit using different functions", "Extract AIC, BIC, R2 values for each pulse signal when fitted with different fitting functions"] |
63,856 | Proflexia RX Male Enhancement - [SCAM Or LEGIT] Report! | 1 | dx.doi.org/10.17504/protocols.io.kxygxz2d4v8j/v1 | https://www.protocols.io/view/proflexia-rx-male-enhancement-scam-or-legit-report-cakqscvw | V V | TITLE: Proflexia RX Male Enhancement - [SCAM Or LEGIT] Report!
AUTHORS: V V
[DESCRIPTION]
Proflexia RX: Does It Work? Alarming Research Revealed! Job – (DONOTUSE) University of California Santa Barbara Police Department.
[STEPS]
1. ➢ Product Name — Proflexia RX Male Enhancement
➢ Composition — Natural Organic Comp... | ["➢ Product Name — Proflexia RX Male Enhancement\n\n➢ Composition — Natural Organic Compound\n\n➢ Side-Effects — NA\n\n➢ Availability — Online\n➢ Rating — ⭐⭐⭐⭐⭐\n\n➢ Official Website (Sale Is Live) — Proflexia RX Male Enhancement\n\n➢VISIT THE OFFICIAL WEBSITE TO BUY TODAY SPECIAL OFFER!!\n\n➢VISIT THE OFFICIAL WEBSIT... |
99,424 | Protocol Normative Gait Data ZHAW Movement Laboratory | 0 | dx.doi.org/10.17504/protocols.io.bp2l621pzgqe/v1 | https://www.protocols.io/view/protocol-normative-gait-data-zhaw-movement-laborat-ddb822rw | Bettina Sommer, Christoph Bauer, Carole Pauli, Michelle Haas, Eveline Graf | TITLE: Protocol Normative Gait Data ZHAW Movement Laboratory
AUTHORS: Bettina Sommer, Christoph Bauer, Carole Pauli, Michelle Haas, Eveline Graf
[DESCRIPTION]
This protocol describes the detailed study procedures for the collection of normative gait data for walking at the ZHAW movement laboratory.
[STEPS]
SECTION: L... | ["[Laboratory Preparation] Cameras\nA total of 12 Vicon Vantage cameras were used for data collection.\nCameras need to be turned on for warming up at least 30 minutes before system calibration", "[Laboratory Preparation] Photocells\nThe photocells are placed in the room as shown in the figure above, with a distance of... |
31,095 | Immediate Early Gene (IEG) mapping of spinal cord neurons activated by cystometry-induced micturition in rats [keast-002] | null | dx.doi.org/10.17504/protocols.io.bakxicxn | https://www.protocols.io/view/immediate-early-gene-ieg-mapping-of-spinal-cord-ne-bakxicxn | Janet Keast, Peregrine Osborne, Nicole Wiedmann | TITLE: Immediate Early Gene (IEG) mapping of spinal cord neurons activated by cystometry-induced micturition in rats [keast-002]
AUTHORS: Janet Keast, Peregrine Osborne, Nicole Wiedmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This collection describes the procedures required to visualize and... | [] |
13,110 | DNA quantification with PicoGreen | 1 | dx.doi.org/10.17504/protocols.io.6qpvrbz3lmkn/v1 | https://www.protocols.io/view/dna-quantification-with-picogreen-q2wdyfe | Alexander Eiler | TITLE: DNA quantification with PicoGreen
AUTHORS: Alexander Eiler
[DESCRIPTION]
The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. Free dye does not fluoresce, but upon binding to dsDNA it exhibits a >1000-fold fluorescence enhancement. PicoGreen is 10,000-fold more sensitive than UV ... | ["Prepare TE\n \n Calculate the total volume of 1xTE needed: \n200 µl per sample\n100 µl per standard\nTotal of 3-400 µl for the DNA standard dilution (depending on the assay you use, see step 2)\nMake enough to count for pipetting losses!!!\nE. g. for high range: 20 samples + 2* 4 standards + DNA standard the amount i... |
36,625 | SPARC Cat - Sham Control Chronic Implant Cat 4, Day 14 | 1 | dx.doi.org/10.17504/protocols.io.bfzrjp56 | https://www.protocols.io/view/sparc-cat-sham-control-chronic-implant-cat-4-day-1-bfzrjp56 | Brett Hanzlicek, Anna Rietsch, Margot Damaser | TITLE: SPARC Cat - Sham Control Chronic Implant Cat 4, Day 14
AUTHORS: Brett Hanzlicek, Anna Rietsch, Margot Damaser
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a procedure for a sham control chronic implant cat experiment (Day 14) for cystotomy (bladder surgery). The cystot... | ["[Transport Cat]\nTransport cat from housing site to surgery site.", "[Animal Prep and catheter placement]\nAnimal is anesthetized and abdomen is shaved by the vet team. The cat is then moved into the surgery room and attached to monitors by the vet team.", "[Animal Prep and catheter placement]\nDrape animal and perf... |
36,581 | Citrate-Phosphate Buffer | null | dx.doi.org/10.17504/protocols.io.bfydjps6 | https://www.protocols.io/view/citrate-phosphate-buffer-bfydjps6 | Neilier Junior | TITLE: Citrate-Phosphate Buffer
AUTHORS: Neilier Junior
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for en... | ["[Citrate-Phosphate Buffer]\nMix citric acid and sodium phosphate solutions in the proportions indicated and adjust the final volume to with deionized water. ABCDEFGHIJKLM1mL of Citric acid44.639.835.932.329.426.724.322.219.716.913.66.52mL of Sodium phosphate5.410.214.117.720.623.325.727.830.333.136.443.63pH2.63.03.... |
27,094 | OSIP 2019 Exercise | null | dx.doi.org/10.17504/protocols.io.6pwhdpe | null | Mathilde Panes | TITLE: OSIP 2019 Exercise
AUTHORS: Mathilde Panes
[STEPS]
?. [Importing and organizing data]
Download data from go.epfl.ch/OSIP-ZEN
?. [Importing and organizing data]
Archive a copy of the data locally.
?. Open a shell / command prompt and move to the folder where the data is stored.Run the following command | ["[Importing and organizing data]\nDownload data from go.epfl.ch/OSIP-ZEN", "[Importing and organizing data]\nArchive a copy of the data locally.", "Open a shell / command prompt and move to the folder where the data is stored.Run the following command"] |
62,932 | Quantitative Method to Measure Disinfectant Product Efficacy | 4 | dx.doi.org/10.17504/protocols.io.kxygxz13zv8j/v1 | https://www.protocols.io/view/quantitative-method-to-measure-disinfectant-produc-b9pur5nw | John Hilgren | TITLE: Quantitative Method to Measure Disinfectant Product Efficacy
AUTHORS: John Hilgren
[DESCRIPTION]
This protocol is used for measuring the inactivation of Pseudomonas aeruginosa by liquid disinfectants. In brief, stainless-steel discs are inoculated with single 10 µL drops of a P. aeruginosa suspension also conta... | ["[Prepare the test culture (minus the soil load)] Prepare the test culture (minus the soil load)", "[Prepare the test culture (minus the soil load)] Prepare tubes of Synthetic Broth (SB) by adding 0.1 mL of 10% sterile dextrose (w/v) solution to each 10 mL tube of SB and mix.", "[Prepare the test culture (minus the so... |
61,538 | Immunofluorescence for Primary Brain Cell Cultures | 1 | dx.doi.org/10.17504/protocols.io.e6nvwkzr9vmk/v1 | https://www.protocols.io/view/immunofluorescence-for-primary-brain-cell-cultures-b8carsse | Ashley V Kumar, Francesca Telese | TITLE: Immunofluorescence for Primary Brain Cell Cultures
AUTHORS: Ashley V Kumar, Francesca Telese
[DESCRIPTION]
Here we describe a protocol to detect specific proteins by immunofluorescence on cells cultured as monolayers. The protocol has been tested on primary cortical neurons and primary astrocytes isolated from ... | ["[Day 1: Fixation, Permeabilization, Blocking, Primary Antibody] Fixation. Aspirate culture media and wash once with ice-cold 1X PBS. Add 4% Formaldehyde fixation buffer. Incubate at room temperature (RT) for 15 min. Use an orbital shaker using low speed.", "[Day 1: Fixation, Permeabilization, Blocking, Primary Antibo... |
null | null | null | dx.doi.org/10.17504/protocols.io.kbfcsjn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span lang="EN-US" style="margin: 0px; line-height: 200%;">Increasing energy expenditure by stimulating thermogenesis through activation of brown adipose tissue (BAT) and/or induction of browning of white adipose tissue (WAT) is considered a promising strategy to treat/preven... | [] |
102,000 | Chromatographic separation of strontium in archaeological human and faunal enamel for Thermal Ionisation Mass Spectrometry (TIMS) analysis | 0 | dx.doi.org/10.17504/protocols.io.bp2l628nkgqe/v1 | https://www.protocols.io/view/chromatographic-separation-of-strontium-in-archaeo-dfuq3nvw | Lisette M. Kootker, Maura De Coster | TITLE: Chromatographic separation of strontium in archaeological human and faunal enamel for Thermal Ionisation Mass Spectrometry (TIMS) analysis
AUTHORS: Lisette M. Kootker, Maura De Coster
[DESCRIPTION]
This protocol describes in great detail all the steps that must be taken for strontium isotope analysis on archae... | ["[Tooth cleaning] If the dental elements are contaminated with soil, place the teeth in glass beaker and submerge with Milli-QTM (Millipore, resistivity >18 Ω/cm. Hereafter: Milli-Q). Clean the teeth ultrasonically for ca. 10 min. Replace the Milli-Q if needed and repeat until the Milli-Q remains clean. Rinse the tee... |
70,192 | Arabidopsis seeds priming | 4 | dx.doi.org/10.17504/protocols.io.kqdg394z1g25/v1 | https://www.protocols.io/view/arabidopsis-seeds-priming-cgsqtwdw | Tao-Ho Chang | TITLE: Arabidopsis seeds priming
AUTHORS: Tao-Ho Chang
[DESCRIPTION]
The treated seeds can grow in medium or bulk soil to determine the impact of novel materials on plants.
[BEFORE_START]
Seed priming is an important method that increases the health of the plant.
[GUIDELINES]
Seed treatment of arabidopsis seeds is ... | ["[Seeds sterilisation] The arabidopsis seeds are immersed with 50% bleach in 1.5 µL tubes.", "[Seeds sterilisation]", "[Seeds sterilisation] 100 rpm, 10 s, 28 °C", "[Seeds sterilisation] Gently remove the supernatant and leave the seeds in the tube.", "[Seeds sterilisation] The sterile seeds are immersed in distilled ... |
88,568 | Global Malaise Trap Project and LIFEPLAN Malaise sampling | 1 | null | https://www.protocols.io/view/global-malaise-trap-project-and-lifeplan-malaise-s-c2qyydxw | Jayme E Sones, Gaia Giedre Banelyte, Jeremy R. deWaard, Arielle M Farrell, Hanna M.K. Rogers, Deirdre Kerdraon, Kate HJ Perez | TITLE: Global Malaise Trap Project and LIFEPLAN Malaise sampling
AUTHORS: Jayme E Sones, Gaia Giedre Banelyte, Jeremy R. deWaard, Arielle M Farrell, Hanna M.K. Rogers, Deirdre Kerdraon, Kate HJ Perez
[DESCRIPTION]
Lifeplan is a global biodiversity monitoring project with the aim of assessing the current state of biodi... | ["[Malaise trap sampling] Label your bottle clearly with the sample location, placement date and other relevant information. Be aware that ethanol erases most markers; to avoid that you can use a pencil.\n\nNote: For the LIFEPLAN project, this information is collected by scanning a QR code in the LIFEPLAN App.", "[Plac... |
null | null | null | dx.doi.org/10.17504/protocols.io.fnvbme6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.h4mb8u6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
67,413 | NTX Nutrition Keto Gummies Reviews – 7 Day Challenge NTX Nutrition Keto Diet + Improve Metabolism! | 4 | dx.doi.org/10.17504/protocols.io.dm6gpbpeplzp/v1 | https://www.protocols.io/view/ntx-nutrition-keto-gummies-reviews-7-day-challenge-cd3vs8n6 | Ntx Nutrition Keto Gummies | TITLE: NTX Nutrition Keto Gummies Reviews – 7 Day Challenge NTX Nutrition Keto Diet + Improve Metabolism!
AUTHORS: Ntx Nutrition Keto Gummies
[DESCRIPTION]
NTX Nutrition Keto Gummies
[STEPS]
1. We all struggle with being overweight because of our horrific eating practices. The herbal approach to losing weight may b... | ["We all struggle with being overweight because of our horrific eating practices. The herbal approach to losing weight may be very hard. Being overweight ought to make life a whole lot more uncomfortable for you. The very last outcome may be fatigue. It is feasible to have fatigue and be no longer successful to finish ... |
89,475 | ISL Opentrons pipeline: gDNA bead cleanup | 4 | dx.doi.org/10.17504/protocols.io.3byl4j9pzlo5/v2 | https://www.protocols.io/view/isl-opentrons-pipeline-gdna-bead-cleanup-c3mbyk2n | Jhakelin Reyes Vasquez, P. Sánchez-Vendizú, Kristina N Vsevolodova, Gideon Erkenswick, Mrinalini Watsa | TITLE: ISL Opentrons pipeline: gDNA bead cleanup
AUTHORS: Jhakelin Reyes Vasquez, P. Sánchez-Vendizú, Kristina N Vsevolodova, Gideon Erkenswick, Mrinalini Watsa
[DESCRIPTION]
This protocol is an automated pipeline to clean a plate of extracted DNA using SPRI bead cleanup. It is functional for both. It is typically u... | ["[BEFORE STARTING] Materials:\nAutoclave and UV the items you will use to ensure sterility. Some items can be autoclaved and reused as indicated below.\n \n ItemquantitycheckstatusAutoclavedUVOpentrons 20µL Filter Tips31NEWOpentrons 200µL Filter Tips21NEW1NEST 1-Well Reservoirs, 195 mL11REUSED11NEST 12-Well Reser... |
22,589 | Single Nuclei extraction from frozen brain | null | dx.doi.org/10.17504/protocols.io.2a5gag6 | null | Bruno Benitez | TITLE: Single Nuclei extraction from frozen brain
AUTHORS: Bruno Benitez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;:UNDERLINE;">Protocol: Purification of nuclei from human brains using an iodixanol gradient</span></div><div class = "text-block">Use BSL2 precauti... | [] |
51,135 | c2 | 2 | null | https://www.protocols.io/view/c2-bv67n9hn | Mariia Guliakina II | TITLE: c2
AUTHORS: Mariia Guliakina II
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">test </div></div>
[STEPS] | [] |
49,308 | VIRUSHUNTER™ ARTIC SARS-CoV-2 Whole Genome Sequencing Assay | 4 | dx.doi.org/10.17504/protocols.io.bud4ns8w | https://www.protocols.io/view/virushunter-artic-sars-cov-2-whole-genome-sequenc-bud4ns8w | Kendall Hoff | TITLE: VIRUSHUNTER™ ARTIC SARS-CoV-2 Whole Genome Sequencing Assay
AUTHORS: Kendall Hoff
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The VirusHunter ARTIC SARS-CoV-2 Whole Genome Sequencing Assay uses a modified version of the sample prep method developed by Josh Quick and the ARTIC Ne... | ["RNA Extraction: this protocol is for the sequencing of SARS-CoV-2 from previously positive patient samples. Leftover RNA from qPCR testing can be used for this assay assuming proper storage/handling.", "RTIn the PCR cabinet, add of the 5xLunaSript RT SuperMix to PCR tubes or plates.Add of your template RNA in a dis... |
24,257 | Small volume fungal genomic DNA extraction protocol for Illumina genome | 1 | dx.doi.org/10.17504/protocols.io.3w9gph6 | https://www.protocols.io/view/small-volume-fungal-genomic-dna-extraction-protoco-3w9gph6 | Jana M U'Ren, Lilly Moore | TITLE: Small volume fungal genomic DNA extraction protocol for Illumina genome
AUTHORS: Jana M U'Ren, Lilly Moore
[DESCRIPTION]
This protocol describes a modified SDS phenol:chloroform method for obtaining high quality DNA from hyphae of filamentous fungi. Resulting DNA can be used for Illumina genome sequencing. P... | ["Pre-measure SDS Buffer need for all samples (e.g. 500uL SDS Buffer x 3 samples= 1,500uL) into a 5 mL tube.\n \n500uL SDS Buffer x ______samples = _______________uL of SDS Buffer pre-aliquoted", "Bead beat tissue frozen tissue via one 15 second cycle at 1400 RPM in frozen aluminum block. If samples are properly homoge... |
97,411 | Liver Tissue RNA Isolation - University of Minnesota TMCs | 0 | dx.doi.org/10.17504/protocols.io.n92ld83yxv5b/v1 | https://www.protocols.io/view/liver-tissue-rna-isolation-university-of-minnesota-dbdb2i2n | Laura J Niedernhofer, David A Bernlohr | TITLE: Liver Tissue RNA Isolation - University of Minnesota TMCs
AUTHORS: Laura J Niedernhofer, David A Bernlohr
[DESCRIPTION]
InvitrogenTM TRIzolTM Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant ce... | ["[Isolate RNA] a. Lyse and homogenize samples in TRIzol Reagent by adding 1mL TRIzol reagent per 50-100mg tissue to the sample and use a homogenizer\nb. Incubate for 5 minutes to permit complete dissociate of the nucleoproteins complex\nc. Add 0.2mL of chloroform per 1mL TRIzol Reagent used for lysis, then securely ca... |
61,800 | Wet Chemistry RNA Isolation Method for Mature Cassava Leaf Tissue | 1 | null | https://www.protocols.io/view/wet-chemistry-rna-isolation-method-for-mature-cass-b8kgrutw | Lynn Doran | TITLE: Wet Chemistry RNA Isolation Method for Mature Cassava Leaf Tissue
AUTHORS: Lynn Doran
[DESCRIPTION]
RNA extraction protocol for cassava for RNA of sufficient quality and quantity to perform RT-PCR gene expression studies.
*Low recovery was achieved using Qiagen RNeasy kit. Typical recoveries using the min... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.skpecvn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Using FACS to sort fluorescent Bodo cells</strong></p>
<p><strong> </strong></p>
<p><strong>Introduction:</strong> Fluorescence-activated cell sorting (<strong>FACS</strong>) is a powerful method to isolate cells expressing fluorescent proteins. It is a routine techni... | [] |
25,265 | Prospective life-style intervention in a CVD risk cohort | null | dx.doi.org/10.17504/protocols.io.4wrgxd6 | null | Lena Haglin, Birgitta Törnkvist, Lennart Bäckman | TITLE: Prospective life-style intervention in a CVD risk cohort
AUTHORS: Lena Haglin, Birgitta Törnkvist, Lennart Bäckman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Abstract</span></div><div class = "text-block">The high CVD-risk population (2504 patients; 1096... | ["The population in VHEC.The patients had a clinical and biochemical assessed diagnosis and were referred from primary care and hospitals in the county (64%, hypertension; 20%, type 2 diabetes; and 55%, BMI >30 (kg/m2). The diagnosis was re-assessed and confirmed by the physician at the health care center. In addition ... |
84,603 | Nuclei Isolation and Immunoprecipitation for 10X Sequencing | 4 | dx.doi.org/10.17504/protocols.io.kxygx9jk4g8j/v2 | https://www.protocols.click/view/nuclei-isolation-and-immunoprecipitation-for-10x-s-cwu3xeyn | Lakme Caceres | TITLE: Nuclei Isolation and Immunoprecipitation for 10X Sequencing
AUTHORS: Lakme Caceres
[DESCRIPTION]
This protocol is for isolating nuclei for downstream sequencing applications.
[GUIDELINES]
Keep tissue/nuclei on ice as much as possible.
[STEPS]
SECTION: Prepare Fresh Solutions
8. Make 3 mL Homogenization Buffe... | ["[Prepare Fresh Solutions] Make 3 mL Homogenization Buffer per sample by adding 2.895 mL Nuclear Isolation Media (filtered via syringe) to a 5 mL eppendorf. Then add 3 μL 100 mM DTT and 30 μL 10% Triton X-100. Add 15 uL RNAsin and invert to mix. Store on ice.", "[Prepare Stock Solutions] Make 20 mL 10% BSA by combinin... |
72,192 | Protocol 3: Growing liquid cultures of Agrobacterium prior to transformation day | 4 | dx.doi.org/10.17504/protocols.io.kqdg399qeg25/v1 | https://www.protocols.io/view/protocol-3-growing-liquid-cultures-of-agrobacteriu-ciq8udzw | Sarah M Prostak, Edgar M Medina, Erik Kalinka, Lillian Fritz-Laylin | TITLE: Protocol 3: Growing liquid cultures of Agrobacterium prior to transformation day
AUTHORS: Sarah M Prostak, Edgar M Medina, Erik Kalinka, Lillian Fritz-Laylin
[DESCRIPTION]
Overnight liquid cultures of Agrobacterium transformed with the plasmid(s) of interest must be prepared the night before Spizellomyces trans... | ["[Steps] Fill the appropriate amount of culture tubes each with 5 mL of LB liquid media.", "[Steps] Using an inoculation loop or pipette tip, gently scrape the surface of the appropriate Agro plate to collect the bacteria or pick the colony of interest.", "[Steps] Swirl the loop or tip to dislodge the bacteria into th... |
53,833 | Agarose gel pads for live Ashbya imaging | 4 | null | https://www.protocols.io/view/agarose-gel-pads-for-live-ashbya-imaging-bythpwj6 | ameya.jalihal , Grace | TITLE: Agarose gel pads for live Ashbya imaging
AUTHORS: ameya.jalihal , Grace
[DESCRIPTION]
A protocol to prepare agarose gel pads for live Ashbya imaging.
[BEFORE_START]
Rinse a depression slide with ethanol and have coverslips on hands.
[STEPS]
SECTION: Resuspension of Ashbya
1.
SECTION: Resuspension of A... | ["[Resuspension of Ashbya]", "[Resuspension of Ashbya] Spin down overnight Ashbya culture to pellet cells in 15 mL conical tubes 5 min 300 RPM", "[Resuspension of Ashbya] Use a pipette to remove as much of the AFM as you can while leaving the cells at the bottom. Resuspend in 4 mL 1X LFM. Spin down again. 5 min 300 RP... |
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