id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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31,006 | The Effect of Waiting on Aggressive Tendencies toward Emergency Department Staff: Providing Information can Help but may also Backfire | null | dx.doi.org/10.17504/protocols.io.bah6ib9e | null | Dorit Treister, Anat Rafaeli, Hadar Moriah | TITLE: The Effect of Waiting on Aggressive Tendencies toward Emergency Department Staff: Providing Information can Help but may also Backfire
AUTHORS: Dorit Treister, Anat Rafaeli, Hadar Moriah
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">4.1.Sample and Data Coll... | [] |
97,996 | Polychromatic UV Fluence (Dose) Response Determination | 1 | dx.doi.org/10.17504/protocols.io.n92ldzqoov5b/v4 | https://www.protocols.io/view/polychromatic-uv-fluence-dose-response-determinati-dbxk2pkw | Daniel Ma, NATALIE HULL | TITLE: Polychromatic UV Fluence (Dose) Response Determination
AUTHORS: Daniel Ma, NATALIE HULL
[DESCRIPTION]
The purpose of this protocol is to document the steps used for determination of UV doses for polychromatic UV sources such as UV LEDs, excimer lamps, medium pressure mercury lamps. The method is not limited to ... | ["[UV Dose Spreadsheet] UV Dose Spreadsheet: \n\nHere is an example spreadsheet filled out with radiometer factors, absorbance scan of sample, UV emission spectra of a low pressure mercury lamp, and sample geometry: \n\nThis protocol will guide users through important parts of the UV Dose Spreadsheet.", "[Performin... |
56,562 | Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System | 4 | dx.doi.org/10.17504/protocols.io.b3gsqjwe | https://www.protocols.io/view/automated-chloroform-methanol-protein-extraction-o-b3gsqjwe | Yan Chen, Tad Ogorzalek, Nurgul Kaplan Lease, Jennifer Gin, Christopher J Petzold | TITLE: Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System
AUTHORS: Yan Chen, Tad Ogorzalek, Nurgul Kaplan Lease, Jennifer Gin, Christopher J Petzold
[DESCRIPTION]
This protocol details steps to extract protein from Gram-negative bacterial or fungal cells (that have been pretrea... | ["[Deck Setup] Open Biomek Software that controls Biomek-FX liquid handler system. Under \"File\" drop-down, click \"Open\" to select the automation method \"Modular Protein Extraction method.\"", "[Deck Setup] Click on \"Instrument Setup\" under the \"Setup\" group node to get visual instruction on how to set up the d... |
89,630 | Kordower Lab Solution Recipes | 4 | dx.doi.org/10.17504/protocols.io.261gedxjjv47/v1 | https://www.protocols.io/view/kordower-lab-solution-recipes-c3r6ym9e | Jeffrey Kordower, Yaping Chu | TITLE: Kordower Lab Solution Recipes
AUTHORS: Jeffrey Kordower, Yaping Chu
[DESCRIPTION]
Kordower Lab Solution Recipes
[STEPS]
SECTION: Slide Subbing Solution
1. Warm 500 mLof dH2O to 50 °C to 60 °C, once at temperature, turn off heat, add and mix until dissolved:
1.25 g Gelatin Type A
0.125 gChromium Potassium Sulfa... | ["[Slide Subbing Solution] Warm 500 mLof dH2O to 50 °C to 60 °C, once at temperature, turn off heat, add and mix until dissolved:\n1.25 g Gelatin Type A\n0.125 gChromium Potassium Sulfate", "[Cryoprotectant Solution] In a 4000 mLbeaker, begin with 2000 mL of 0.2 Molarity (m) Phosphate-Buffered Saline (PBS), pH 7.2\n(or... |
69,420 | Guidance for populating GenomeTrakr metadata templates (BioSample and SRA) | 1 | dx.doi.org/10.17504/protocols.io.eq2ly3x1pgx9/v9 | https://www.protocols.io/view/guidance-for-populating-genometrakr-metadata-templ-cf2ktqcw | Ruth Timme, Maria Balkey, William Wolfgang, Errol Strain | TITLE: Guidance for populating GenomeTrakr metadata templates (BioSample and SRA)
AUTHORS: Ruth Timme, Maria Balkey, William Wolfgang, Errol Strain
[DESCRIPTION]
PURPOSE: Guidance on how to populate NCBI's metadata packages, maximizing interoperability for foodborne pathogen surveillance.
SCOPE: This protocol provi... | ["[Overview] Guidance for organizing and populating the metadata templates required for direct submission to NCBI. This guidance is applicable for most enterics and/or microbial pathogens. \n\n****If your laboratory uses the BioNumerics platform for submission, please follow this protocol.****\n\nTwo metadata template... |
75,162 | OSU TriState SenNet Processing and Storing of Explanted IPF Lungs | 1 | dx.doi.org/10.17504/protocols.io.e6nvwjz87lmk/v1 | https://www.protocols.io/view/osu-tristate-sennet-processing-and-storing-of-expl-cmm2u48e | Sean D. Stacey, Brenda F. Reader, Lorena Rosas, Victor Peters, Ana L. Mora, Mauricio Rojas | TITLE: OSU TriState SenNet Processing and Storing of Explanted IPF Lungs
AUTHORS: Sean D. Stacey, Brenda F. Reader, Lorena Rosas, Victor Peters, Ana L. Mora, Mauricio Rojas
[DESCRIPTION]
This protocol describes the processing and storing of explanted idiopathic pulmonary fibrosis (IPF) lungs by the Comprehensive Trans... | ["[Objective] To preserve lung tissue for further downstream cellular, protein, RNA, or DNA analyses.", "[Preparation] Use appropriate PPE that includes nitrile gloves and disposable gown or washable lab coat.", "[Preparation] Place three underpads and all needed equipment, including biohazard, receptacles, surgical ki... |
70,857 | Human induced pluripotent stem cell culture | 1 | dx.doi.org/10.17504/protocols.io.81wgby7dnvpk/v1 | https://www.protocols.io/view/human-induced-pluripotent-stem-cell-culture-chfht3j6 | gurvir.virdi | TITLE: Human induced pluripotent stem cell culture
AUTHORS: gurvir.virdi
[DESCRIPTION]
Cell culture for the maintenance of hiPSCs.
[STEPS]
1. Coat 6 well plates with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific), 1:100 dilution for 1 hour before cell passaging.
2. When h... | ["Coat 6 well plates with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific), 1:100 dilution for 1 hour before cell passaging.", "When hiPSCs are 80-90% confluent, they are passaged:", "Old media is aspirated and replaced with 1ml of cell-culture PBS (Gibco, Thermo Fisher Scient... |
103,077 | Purification GFP-ATG13 IDR | 0 | dx.doi.org/10.17504/protocols.io.8epv5rey4g1b/v1 | https://www.protocols.io/view/purification-gfp-atg13-idr-dgwd3xa6 | Elias Adriaenssens | TITLE: Purification GFP-ATG13 IDR
AUTHORS: Elias Adriaenssens
[DESCRIPTION]
This protocol details the purification of GFP-ATG13 IDR.
[STEPS]
SECTION: Purification
1. To purify GFP-tagged ATG13 IDR, the coding sequence for ATG13 (190-517aa), (206-517aa), (231-517aa), (190-205_231-517aa), (190-230aa), (190-205aa), or (... | ["[Purification] To purify GFP-tagged ATG13 IDR, the coding sequence for ATG13 (190-517aa), (206-517aa), (231-517aa), (190-205_231-517aa), (190-230aa), (190-205aa), or (206-230aa) into GST-TEV-EGFP-insert through cloning into a pGEX-4T1 vector (Plasmids available from Addgene).", "[Purification] Mutants \n\n3A (M196A/S... |
20,659 | UC Davis - Blood Pressure by Tail Cuff | null | dx.doi.org/10.17504/protocols.io.yetften | null | Jennifer Rutkowsky | TITLE: UC Davis - Blood Pressure by Tail Cuff
AUTHORS: Jennifer Rutkowsky
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span><span style = "font-weight:bold;"> </span></div><div class = "text-block">This technique has been used routinely for the non-inva... | ["Mouse configuration:\n1. Place mouse into an appropriate sized restrainer.\n2. Place the tail through the groove of the back of the restrainer and tighten screw.\n3. Feed the tail through the occlusion cuff first and then through the VPR cuff second. Ensure a snug fit but do not force the tail through either cuff as ... |
50,441 | Nuclei isolation from fresh and frozen brain tissue - for single nucleus RNAseq or 10x Multiome | 3 | dx.doi.org/10.17504/protocols.io.bvhhn336 | https://www.protocols.io/view/nuclei-isolation-from-fresh-and-frozen-brain-tissu-bvhhn336 | Isabelle Schmutz, Jade Carter | TITLE: Nuclei isolation from fresh and frozen brain tissue - for single nucleus RNAseq or 10x Multiome
AUTHORS: Isabelle Schmutz, Jade Carter
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">This protocol is for nuclei isolation from frozen mouse brain... | [] |
72,193 | Protocol 4: Creating depressions in induction media plates | 4 | dx.doi.org/10.17504/protocols.io.ewov1oodylr2/v1 | https://www.protocols.io/view/protocol-4-creating-depressions-in-induction-media-ciq9udz6 | Sarah M Prostak, Edgar M Medina, Erik Kalinka, Lillian Fritz-Laylin | TITLE: Protocol 4: Creating depressions in induction media plates
AUTHORS: Sarah M Prostak, Edgar M Medina, Erik Kalinka, Lillian Fritz-Laylin
[DESCRIPTION]
Transformation is carried out by co-culturing Agro and Spizellomyces on induction media (“IM”). Once its virulence genes are induced, Agro will infect Spizellomyc... | ["[Steps] On the bottom, divide an IM plate into quadrants.", "[Steps] Draw circles 2.5 mm in diameter in each quadrant.", "[Steps] Place a 20-30 mm diameter glass culture tube into a 50 mL conical tube.", "[Steps] Add enough 70% ethanol to cover the first inch of the culture tube.", "[Steps] Burn off the ethanol on th... |
40,284 | Chromatin Immunoprecipitation | 4 | dx.doi.org/10.17504/protocols.io.bjj4kkqw | https://www.protocols.io/view/chromatin-immunoprecipitation-bjj4kkqw | Georgios I Laliotis, Philip N. Tsichlis | TITLE: Chromatin Immunoprecipitation
AUTHORS: Georgios I Laliotis, Philip N. Tsichlis
[STEPS]
?. [Chemicals Required]
1. Cytosolic Lysis Buffer (200 ml) ; 5 mM PIPES (pH 8.0), 85 mM KCl, 0.5% NP40 + PI PIPES 0.3 g =>Adjust pH 8.0 2M KCl 8.5 ml 10% NP40 10 ml 2. Nuclear Lysis Buff... | ["[Chemicals Required]\n1. Cytosolic Lysis Buffer (200 ml) ; 5 mM PIPES (pH 8.0), 85 mM KCl, 0.5% NP40 + PI PIPES 0.3 g =>Adjust pH 8.0 2M KCl 8.5 ml 10% NP40 10 ml 2. Nuclear Lysis Buffer (50 ml) ; 50 mM Tris (pH 8.0), 10 mM EDTA, 0.5% SDS 1 M Tris (pH 8.0) 2... |
95,602 | Protocol for "non-human primate necropsy" | 0 | dx.doi.org/10.17504/protocols.io.rm7vzxzw5gx1/v1 | https://www.protocols.io/view/protocol-for-34-non-human-primate-necropsy-34-c9ksz4we | jlanciego | TITLE: Protocol for "non-human primate necropsy"
AUTHORS: jlanciego
[DESCRIPTION]
Here we describe the standard procedure conducted for performing a non-human primate necropsy, followed by removal of the brain and spinal cord from the skull and backbone, respectively.
[STEPS]
SECTION: Animal preparation
1. Pr... | ["[Animal preparation] Pre-surgical anesthesia: to be induced with ketamine (5 mg/Kg) and midazolam (0.5 mg/Kg), administered intramuscularly", "[Animal preparation] Terminal anesthesia: to be induced with an overdose of sodium pentobarbital (200 mg/Kg).", "[Prefusion and pre-histological processing] Perform a T-shape ... |
95,760 | Mycota Lab CTAB Protocol | 0 | dx.doi.org/10.17504/protocols.io.yxmvm383ol3p/v1 | https://www.protocols.io/view/mycota-lab-ctab-protocol-c9rqz55w | Stephen Douglas Russell | TITLE: Mycota Lab CTAB Protocol
AUTHORS: Stephen Douglas Russell
[DESCRIPTION]
This protocol is an amalgamation of the methods used by Osmundson et al. and Forin et al. for fungal herbarium specimens.
[STEPS]
SECTION: DNA Extraction Procedure
1. Create your stock Proteinase K and RNASE A solutions. Both at 20mg / mL.... | ["[DNA Extraction Procedure] Create your stock Proteinase K and RNASE A solutions. Both at 20mg / mL. So for 100mg of Proteinase K, add 5 mL of molecular water. Same for the RNASE A.", "[DNA Extraction Procedure] Prep your CTAB buffer in 50mL tubes. Add 500 uL of CTAB buffer per sample. 96 samples add 48mL of CTAB. Fo... |
18,147 | bright field standard swarming imaging | null | dx.doi.org/10.17504/protocols.io.vybe7sn | null | Serena Ding | TITLE: bright field standard swarming imaging
AUTHORS: Serena Ding
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">For imaging swarming behaviour of 40 young adult C. elegans on agar using the Phoenix multi-worm tracker system. Worms are synchronised by bleaching and refeeding for 72 hours, and then... | ["[Imaging plate preparation (Day -7)]\nA separate batch of imaging plates is poured exactly seven days before each imaging day and stored at 4°C.\nImaging plates are 35 mm Petri dishes containing 3.5 mL low peptone (0.013% Difco Bacto) NGMagar (2% Bio/Agar, BioGene) to limit bacteria growth.", "[Imaging plate preparat... |
97,108 | USDA LTAR Common Experiment measurement: Overland flow | 1 | dx.doi.org/10.17504/protocols.io.kxygxyk2zl8j/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-overland-f-da3u2gnw | Kevin J Cole, Anthony R. Buda, Pamela J. Rice, Claire Baffaut | TITLE: USDA LTAR Common Experiment measurement: Overland flow
AUTHORS: Kevin J Cole, Anthony R. Buda, Pamela J. Rice, Claire Baffaut
[DESCRIPTION]
Overland flow is water that flows over a land surface (sheet flow) or in rills and gullies (concentrated flow) in response to rainfall or melting snow. Measuring overland f... | ["[1.\tData collection] Measurement\n\nCollect sensor data every 5 minutes. More frequent measurements may be desirable in areas of very rapid runoff response.", "[1.\tData collection] Site Maintenance", "[1.\tData collection] Visit weekly to ensure sensors (rain gauge and water level), flumes, and weirs are clear of d... |
28,424 | Haematoxylin and eosin staining | null | dx.doi.org/10.17504/protocols.io.7zghp3w | null | Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Abdul Rahman Omar, Aini Ideris, Nurulfiza Mat Isa | TITLE: Haematoxylin and eosin staining
AUTHORS: Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Abdul Rahman Omar, Aini Ideris, Nurulfiza Mat Isa
[STEPS] | [] |
65,701 | Soil sample(Citizen scientists, Chinese) | 1 | dx.doi.org/10.17504/protocols.io.kqdg3py21l25/v2 | https://www.protocols.io/view/soil-sample-citizen-scientists-chinese-ccedsta6 | Hsin-Mao Wu | TITLE: Soil sample(Citizen scientists, Chinese)
AUTHORS: Hsin-Mao Wu
[DESCRIPTION]
Soil sample, citizen scientist
[STEPS]
SECTION: 採土
1. 選定地點，分為以下三種
醫院
公園
天然地區
SECTION: 採土
2. 移除表土，取距離土表5~7公分之處的土，裝入塑膠夾鏈袋中
SECTION: 採土
3. 將塑膠袋中的土均勻混合，倒入HDPE塑膠罐之中
SECTION: 採土
4. 將HDPE罐子收進袋子中
SECTION: 採土
5. 最後拍兩張照片
| ["[採土] 選定地點，分為以下三種\n醫院\n公園\n天然地區", "[採土] 移除表土，取距離土表5~7公分之處的土，裝入塑膠夾鏈袋中", "[採土] 將塑膠袋中的土均勻混合，倒入HDPE塑膠罐之中", "[採土] 將HDPE罐子收進袋子中", "[採土] 最後拍兩張照片"] |
94,501 | Human pluripotent stem cell culture | 1 | dx.doi.org/10.17504/protocols.io.j8nlkoq56v5r/v1 | https://www.protocols.io/view/human-pluripotent-stem-cell-culture-c8idzua6 | Jiuchun Zhang, Harper JW | TITLE: Human pluripotent stem cell culture
AUTHORS: Jiuchun Zhang, Harper JW
[DESCRIPTION]
This protocol is about human pluripotent stem cell culture.
Some facts about human pluripotent stem cells
They attach to several extra cellular matrices, including Matrigel, laminin, vitronectin, fibronectin and high-density R... | ["[Thaw frozen cell from liquid nitrogen tank]", "[Thaw frozen cell from liquid nitrogen tank] Take a vial of frozen cells from liquid nitrogen tank. If you have many vials you need to take out of the tank and thaw at a time, you can put them on dry ice while you are looking for other vials.", "[Thaw frozen cell from l... |
24,611 | GG1 - sgRNA cloning for Phaeodactylum tricornutum | null | dx.doi.org/10.17504/protocols.io.4abgsan | null | Mark Moosburner, Andrew Allen | TITLE: GG1 - sgRNA cloning for Phaeodactylum tricornutum
AUTHORS: Mark Moosburner, Andrew Allen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The CRISPR-Cas9 gene mutagenesis system was adapted for the marine diatom Phaeodactylum tricornutum (CCAP-1055/1) (Figure 1). Here, Cas9 and sgRNA(s) were ... | [] |
44,665 | Maxpar MCP9 cadmium labeling | 1 | null | https://www.protocols.io/view/maxpar-mcp9-cadmium-labeling-bpuzmnx6 | Albert Tsai | TITLE: Maxpar MCP9 cadmium labeling
AUTHORS: Albert Tsai
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Antibody labeling with cadmium isotopes</div></div>
[STEPS]
?. [Preparation]
Bring all buffer solutions to room temperature.
?. [Polymer loading and wash 1]
Thaw Maxpar MCP9 polymer to room temp... | ["[Preparation]\nBring all buffer solutions to room temperature.", "[Polymer loading and wash 1]\nThaw Maxpar MCP9 polymer to room temperature before opening to avoid moisture condensation.", "[Preparation]\nCentrifuge the stock antibody at 12,000 × g for 5 minutes to sediment antibody aggregates, and then verify the s... |
null | null | null | dx.doi.org/10.17504/protocols.io.kzrcx56 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The presence and structure of EFNs in <em>Opuntia robusta</em> had not been investigated. We used light, scanning-electron, and transmission-electron microscopy to examine morphology, anatomy, and ultrastructure of the secretory spines in areoles in female and hermaphrodite i... | [] |
41,724 | Sampleholder preparation | 4 | dx.doi.org/10.17504/protocols.io.bky4kxyw | https://www.protocols.io/view/sampleholder-preparation-bky4kxyw | philippe.bechtold | TITLE: Sampleholder preparation
AUTHORS: philippe.bechtold
[STEPS]
?. The sampleholder contains 9 wells that are preloaded with an RT-qPCR Mastermix as follows:
?. Wells 1 to 3 contain probes and primers for the N1 target, wells 2 to 6 contain probes and primers for the N2 target, wells 7 to 9 contain probes and prim... | ["The sampleholder contains 9 wells that are preloaded with an RT-qPCR Mastermix as follows:", "Wells 1 to 3 contain probes and primers for the N1 target, wells 2 to 6 contain probes and primers for the N2 target, wells 7 to 9 contain probes and primers for the Human RP target. Wells 7,8,9 contain the respective positi... |
45,866 | Fluorescence In Situ Hybridization (FISH - RNAscope) in mouse brain sections | 4 | dx.doi.org/10.17504/protocols.io.bq2imyce | https://www.protocols.io/view/fluorescence-in-situ-hybridization-fish-rnascope-i-bq2imyce | Yu Lin Tan, Oriol Pavón Arocas, Lucille Duquenoy, Tiago Branco | TITLE: Fluorescence In Situ Hybridization (FISH - RNAscope) in mouse brain sections
AUTHORS: Yu Lin Tan, Oriol Pavón Arocas, Lucille Duquenoy, Tiago Branco
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Here we describe a protocol to perform fluorescence in situ hybridization (FISH) in thin section... | ["[Day 0 | Brain extraction]\n[1. Preparation ~ 15 min]\nYou can extract and freeze several brains on the same day and then store them at -80°C until slicing. You can do the brain extraction either in the PFA perfusion room (with tools from there) or in the slice electrophysiology area where you prepare acute brain sli... |
87,222 | noesypr1d_metab.nan | 5 | dx.doi.org/10.17504/protocols.io.x54v9p21pg3e/v1 | https://www.protocols.io/view/noesypr1d-metab-nan-czewx3fe | NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison | TITLE: noesypr1d_metab.nan
AUTHORS: NAN KB, John Glushka, Mario Uchimiya, Saraa Al Jawad, Christopher Esselman, Leandro I Ponce, Laura Morris, Arthur Edison
[DESCRIPTION]
This is a protocol for running the Bruker pulse program "noesypr1d".
[BEFORE_START]
This protocol assumes your sample is loaded, locked, tuned, and... | ["[Create a new dataset]", "[Create a new dataset] On the menu bar on TopSpin, click on\nStart → Create Dataset", "[Create noesypr1d experiment file] When Lock, Tune, Shim are complete proceed to the specific protocol for your desired pulseprogram(s).", "[Create a new dataset] A new window opens. On the right top bar, ... |
85,719 | Nuclei Isolation and Affinity Purification for 10X Sequencing | 4 | dx.doi.org/10.17504/protocols.io.4r3l22ojpl1y/v1 | https://www.protocols.io/view/nuclei-isolation-and-affinity-purification-for-10x-cxxxxppn | Lakme Caceres | TITLE: Nuclei Isolation and Affinity Purification for 10X Sequencing
AUTHORS: Lakme Caceres
[DESCRIPTION]
This protocol is for isolating nuclei for downstream sequencing applications.
[GUIDELINES]
Keep tissue/nuclei on ice as much as possible.
[STEPS]
SECTION: Prepare Fresh Solutions
8. Make 3 mL Homogenization Buf... | ["[Prepare Fresh Solutions] Make 3 mL Homogenization Buffer per sample by adding 2.9 mL Nuclear Isolation Media (filtered via syringe) to a 5 mL eppendorf. Then add 3 μL 100 mM DTT and 30 μL 10% Triton X-100. Add 15 uL RNAsin and invert to mix. Store on ice.", "[Prepare Stock Solutions] Make 20 mL 10% BSA by combining ... |
94,969 | Metaphase_spread_and_DNA_FISH_cell_lines | 4 | dx.doi.org/10.17504/protocols.io.ewov1q6pygr2/v1 | https://www.protocols.io/view/metaphase-spread-and-dna-fish-cell-lines-c8yzzxx6 | jingting | TITLE: Metaphase_spread_and_DNA_FISH_cell_lines
AUTHORS: jingting
[DESCRIPTION]
Cytogenetically detect single-cell ecDNAs in cell lines via staining/homologous DNA hybridization and fluorescence microscopy -- fluorescence in situ hybridization (FISH).
[STEPS]
SECTION: Introduction
1. Cytogenetically profile single-ce... | ["[Introduction] Cytogenetically profile single-cell DNAs through metaphase karyotyping by DNA FISH.", "[Material] Methanol/glacial acetic acid 3:1 -- Prepare fresh in a fume hood prior to use\nHypotonic stock solution: 0.075M KCl (gibco, ref. 10575-0821)\n2 X SSC (Saline-Sodium Citrate) stock solution (/0.05% TWEEN20)... |
48,115 | pcp-1808 test 1 | 1 | null | https://www.protocols.io/view/pcp-1808-test-1-bs8tnhwn | Maria Guliakina | TITLE: pcp-1808 test 1
AUTHORS: Maria Guliakina
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">test</div></div>
[STEPS]
?. test | ["test"] |
null | null | null | dx.doi.org/10.17504/protocols.io.chet3d | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
58,928 | Pole Test | 4 | dx.doi.org/10.17504/protocols.io.b5sqq6dw | https://www.protocols.io/view/pole-test-b5sqq6dw | Haley Geertsma | TITLE: Pole Test
AUTHORS: Haley Geertsma
[DESCRIPTION]
This protocol is used to test mice in the Pole behavioural test.
[STEPS]
1. Habituate mice in the testing room for 60 minutes.
2. Place mice ~3cm from the top of the pole, facing upwards, and record their time to turn facing downwards and their time to reach th... | ["Habituate mice in the testing room for 60 minutes.", "Place mice ~3cm from the top of the pole, facing upwards, and record their time to turn facing downwards and their time to reach the bottom of the pole. The mice have up to 1 minute to turn and 1 minute to reach the bottom before being returned to their home cage.... |
7,166 | WetLab-2 RT-qPCR procedure | null | dx.doi.org/10.17504/protocols.io.i86chze | null | Jimmy Jung, Macarena Parra | TITLE: WetLab-2 RT-qPCR procedure
AUTHORS: Jimmy Jung, Macarena Parra
[DESCRIPTION]
<p>Procedure used for RT-qPCR in the WetLab-2 study</p>
[STEPS]
?. [qPCR]
Preparation of qPCR reactions (per 25ul reaction):Promega GoTaq Probe Master Mix (2X): 12.5 ulForward Primer (20X): 1 ulReverse Primer (20X): 1 ulHydrolysis Pro... | ["[qPCR]\nPreparation of qPCR reactions (per 25ul reaction):Promega GoTaq Probe Master Mix (2X): 12.5 ulForward Primer (20X): 1 ulReverse Primer (20X): 1 ulHydrolysis Probe (20X): 1 ulDNA Template: varied from 1 pg to 1ug per reactionMolecular Biology Grade Water: to 25 ul total volume", "[RT-qPCR]\nPreparation of RT-q... |
21,429 | Squalene Quantification using Nile Red Staining (M4455 Version) | null | dx.doi.org/10.17504/protocols.io.y6vfze6 | null | Sebastian Triesch | TITLE: Squalene Quantification using Nile Red Staining (M4455 Version)
AUTHORS: Sebastian Triesch
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">This protocol is under development and for teaching purposes only!</span></div><div class = "text-block">Nile Red is a f... | ["Sample 1-2 ml Synechocystis culture, measure its OD at 750 nm and adjust it to 2 ml of OD (750 nm) = 0.2 in BG-11 media. Split your adjusted culture in 2x 1 ml. One portion will be stained with Nile Red, the other will serve as a negative control.", "Stain one portion of the previously adjusted culture with c(final) ... |
26,316 | Seeding 90mm NGM plates with bacteria | null | dx.doi.org/10.17504/protocols.io.5xkg7kw | null | Cristian Riccio | TITLE: Seeding 90mm NGM plates with bacteria
AUTHORS: Cristian Riccio
[STEPS]
?. At the Bunsen burner, transfer the bacterial culture to a 50 ml Falcon tube in order to make it possible for the repeat combitip pipetter to reach the bacterial culture. Do so using a serological pipette and a pipetboy.
?. Set the repeat ... | ["At the Bunsen burner, transfer the bacterial culture to a 50 ml Falcon tube in order to make it possible for the repeat combitip pipetter to reach the bacterial culture. Do so using a serological pipette and a pipetboy.", "Set the repeat pipetter to dispense 600 ul at each push.", "Aspire 10 ml of bacterial culture i... |
92,762 | SiMOA pT73-Rab10 Homebrew Assay | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdn69lmk/v1 | https://www.protocols.io/view/simoa-pt73-rab10-homebrew-assay-c6t2zeqe | yuan.yuan, andrew.west | TITLE: SiMOA pT73-Rab10 Homebrew Assay
AUTHORS: yuan.yuan, andrew.west
[DESCRIPTION]
Assay for the detection of pT73-Rab10 in biofluids and lysates
[STEPS]
2. Prepare the Beads
3. Conjugate
SECTION: Prepare Capture Beads Concentrate (5-6 hours)
1. Prepare capture beads using a two-step EDC coupling protocol. Reaction... | ["Prepare the Beads", "Conjugate", "[Prepare Capture Beads Concentrate (5-6 hours)] Prepare capture beads using a two-step EDC coupling protocol. Reaction occurs between the antibody primary amino groups (-NH2) and the carboxyl groups (-COOH) on the beads. 80ug of antibody is required for 0.2 mg/ml buffer exchanged ant... |
22,327 | Sampling macrofungi using fixed-sized plots | null | dx.doi.org/10.17504/protocols.io.z2xf8fn | null | Gregory M. Mueller, John Paul Schmit, Sabine M. Huhndorf, Leif Ryvarden, Thomas E. O'Dell, D. Jean Lodge, Patrick R. Leacock, Milagro Mata, Loengrin Umana, Qiuxin (Florence) Wu, and Daniel L. Czederpilz, Daniel L. Czederpiltz | TITLE: Sampling macrofungi using fixed-sized plots
AUTHORS: Gregory M. Mueller, John Paul Schmit, Sabine M. Huhndorf, Leif Ryvarden, Thomas E. O'Dell, D. Jean Lodge, Patrick R. Leacock, Milagro Mata, Loengrin Umana, Qiuxin (Florence) Wu, and Daniel L. Czederpilz, Daniel L. Czederpiltz
[DESCRIPTION]
<div class = "text-... | ["The investigator selects an area within the site for the permanent plot. The area should be representative of an important forest or grassland type at the site and should be chosen to optimize the diversity of habitat types sampled by the entire study (i.e., if the study covers grasslands, open woodlands, and dense f... |
null | null | null | dx.doi.org/10.17504/protocols.io.m4hc8t6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><span style="font-weight: 400;">Immunohistochemistry (IHC) is a method that combines biochemical, histological and immunological techniques into a simple but powerful assay for protein detection. IHC provides valuable information as it visualizes the distribution and localiza... | [] |
28,494 | Staining cells with IncuCyte Cytolight Rapid Dyes for flow cytometry or fluorescent microscopy | null | dx.doi.org/10.17504/protocols.io.73nhqme | null | Elinor Gottschalk, Bulent Arman Aksoy, Pinar Aksoy, Eric Czech, Jeff Hammerbacher | TITLE: Staining cells with IncuCyte Cytolight Rapid Dyes for flow cytometry or fluorescent microscopy
AUTHORS: Elinor Gottschalk, Bulent Arman Aksoy, Pinar Aksoy, Eric Czech, Jeff Hammerbacher
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for labeling cells with IncuCyte Cytolight... | ["Harvest cells and wash with 1X PBS", "Centrifuge\nCentrifuge: 350 34", "Add IncuCyte Dye For pmel-1 T cells: we determined that 0.11 µM of green dye was optimal We made our working stock 11 µM (so for every 1 ml of cell suspension, we add 10 ul of working stock of dye)For Jurkat cells, 1 µM of green dye was optimal ... |
98,221 | Behavior Hardware Setup Protocol | 0 | dx.doi.org/10.17504/protocols.io.ewov19857lr2/v1 | https://www.protocols.io/view/behavior-hardware-setup-protocol-db6m2rc6 | Sasha Burwell | TITLE: Behavior Hardware Setup Protocol
AUTHORS: Sasha Burwell
[DESCRIPTION]
This protocol details instructions for recreating the conditioning/extinction reward learning assay used in this paper.
[GUIDELINES]
To run the conditioning/extinction reward learning assay, any behavioral setup that incorporates head-fixati... | ["[Behavior Hardware Setup] Use the spray adhesive to attach egg crate foam to the inside walls and ceiling of the Med-Associates box. Though the box is already sound attenuating, this will help with further sound attenuation.", "[Behavior Hardware Setup] Set up the NI Card:", "[Behavior Hardware Setup] Place the NI US... |
63,405 | Optimus Gel- *Ефективни састојци* за смањење болова у зглобовима! Производ за свакодневну употребу | 1 | dx.doi.org/10.17504/protocols.io.8epv59m64g1b/v1 | https://www.protocols.io/view/optimus-gel-b96mr9c6 | Optimus Gel | TITLE: Optimus Gel- *Ефективни састојци* за смањење болова у зглобовима! Производ за свакодневну употребу
AUTHORS: Optimus Gel
[DESCRIPTION]
Оптимус Гел је нови квалитетан производ који обједињује најновија научна достигнућа која заувек ублажавају и отклањају болове у зглобовима, костима и мишићима! Наруч... | ["[Optimus Gel- *Ефективни састојци* за смањење болова у зглобовима! Производ за свакодневну употребу] Optimus Gel Коментара - је амишка редовна ставка за рад на добробити и преносивости зглобова. Под претпоставком да сте истражили сваки избор од лекова преко стероида до медицинске процедуре и да нисте пронашли њихову ... |
null | null | null | dx.doi.org/10.17504/protocols.io.dyw7xd | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This TE buffer contains 10 mM Tris and 0.1 mM EDTA for storage of nucleic acids. Concentration of EDTA is lower than other formulations of TE buffer so that EDTA will not inhibit Taq Polymerase in downstream PCR reactions.
[STEPS] | [] |
105,242 | proteomics (mitochondria) | 0 | dx.doi.org/10.17504/protocols.io.36wgqny15gk5/v1 | https://www.protocols.io/view/proteomics-mitochondria-diz24f8e | Livia Hecke Morais, Baiyi Quan, Tsui-fen Chou | TITLE: proteomics (mitochondria)
AUTHORS: Livia Hecke Morais, Baiyi Quan, Tsui-fen Chou
[DESCRIPTION]
Here we describe proteomics experiment with isolated mitochondrial extracts at the Proteome exploration laboratory (PEL) at Caltech by Baiyi Quan, Jeff Jones and Tsui-Fen Chou in collaboratin with Livia Hecke Morais a... | ["[Sample Preparation] Proteins extracted from mitochondria are reduced using a final concentration of 5 mM of TCEP (tris(2-carboxyethyl)phosphine) at room temperature for 10 min.\n The proteins are further alkylated using a final concentration of 20 mM of CAA (chloro-acetamide) at room temperature for 15 min.\n ... |
44,860 | Enterococcus faecalis protoplast generation | 4 | null | https://www.protocols.io/view/enterococcus-faecalis-protoplast-generation-bp24mqgw | Elizabeth Fozo | TITLE: Enterococcus faecalis protoplast generation
AUTHORS: Elizabeth Fozo
[STEPS]
?. [Protocol]
Grow OG1RF in BHI + condition until OD600 0.3. This works for as little as 10mls of culture.
Growing in 2% glycine will reduce “gluing” of cells, but massively increase generation time.
?. [Protocol]
Spin cells down and re... | ["[Protocol]\nGrow OG1RF in BHI + condition until OD600 0.3. This works for as little as 10mls of culture.\nGrowing in 2% glycine will reduce “gluing” of cells, but massively increase generation time.", "[Protocol]\nSpin cells down and resuspend in half volume isotonic solution.", "[Protocol]\nAdd 2mg/ml lysozyme and i... |
29,339 | TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v2 | null | dx.doi.org/10.17504/protocols.io.8v3hw8n | null | Sam Li | TITLE: TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v2
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the... | ["[I) Cell staining for Drop-seq or 10x Genomics platforms]\nObtain all single cell suspensions from different samples/conditions that will be multiplexed in the run.Keep samples in separate tubes until after cell hashing and shortly before loading cells into the single cell RNA-seq instrument.When aiming to super-load... |
96,879 | 10 X Visium Spatial Gene Expression - Fixed Frozen Tissue Processing with CytAssist | 0 | dx.doi.org/10.17504/protocols.io.q26g71z49gwz/v1 | https://www.protocols.io/view/10-x-visium-spatial-gene-expression-fixed-frozen-t-daup2evn | Alberto Pappalardo, Kavya Batra, Rolando Perez-Lorenzo, Angela Christiano | TITLE: 10 X Visium Spatial Gene Expression - Fixed Frozen Tissue Processing with CytAssist
AUTHORS: Alberto Pappalardo, Kavya Batra, Rolando Perez-Lorenzo, Angela Christiano
[DESCRIPTION]
Here we summarize the reccomendation released by 10X Genomics for processing fixed-frozen tissue sections while performing 10X Visi... | ["[Coverslipping] Once the coverslip settles, immediately proceed with imaging or store slide laying flat at 4°C in the dark for up to two weeks. If storing multiple slides avoid any contact between slides.\nDO NOT exceed two weeks of storage time.", "[Coverslipping] Place the coverslip without introducing bubbles and ... |
66,559 | Olympic Weightlifting Lifts and Derivatives for Fatigue Impact Quantification | 1 | dx.doi.org/10.17504/protocols.io.n92ldzxq8v5b/v1 | https://www.protocols.io/view/olympic-weightlifting-lifts-and-derivatives-for-fa-cc87szzn | J. P. Brito, Rafael Oliveira, paulojet | TITLE: Olympic Weightlifting Lifts and Derivatives for Fatigue Impact Quantification
AUTHORS: J. P. Brito, Rafael Oliveira, paulojet
[DESCRIPTION]
Load management is an extremely important subject in the control of fatigue and adaptation process in almost all sports. In Olympic Weightlifting (OW), some of the load va... | ["Protocol Test\n 1. A standardized 10 minutes warm-up including, mobility exercises, several OW repetitions and jumps was carried out to all experimental groups before the beginning of each training session, to minimize the risk of injury, additionally there were always two assistants to monitor exercise execution.\n ... |
42,088 | Introduction to Materials | 3 | null | https://www.protocols.io/view/introduction-to-materials-bmcgk2tw | Alyssa Ayala | TITLE: Introduction to Materials
AUTHORS: Alyssa Ayala
[STEPS] | [] |
44,540 | Anaerobic Media Preparation Protocol | 4 | null | https://www.protocols.io/view/anaerobic-media-preparation-protocol-bpq4mmyw | Orkun Soyer | TITLE: Anaerobic Media Preparation Protocol
AUTHORS: Orkun Soyer
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is a generic protocol for the preparation of </span><span style = "font-style:italic;">any</span><span> strictly anaerobic media. The exact media composition is assumed to be k... | ["[Prep. and mixing stage]\nIn dissolve the salt mix of the medium, while stirring the liquid on a stirrer plate.\n[milliq-H2O]\nThis dissolved salt mix can be stored without any problem (e.g. contamination would not be expected as it does not contain any carbon source, most salts should be stable, etc.)", "[Prep. and... |
63,386 | Hazel Hills CBD Gummies Review | 3 | dx.doi.org/10.17504/protocols.io.e6nvwk269vmk/v1 | https://www.protocols.io/view/hazel-hills-cbd-gummies-review-b952r88e | NanicLews | TITLE: Hazel Hills CBD Gummies Review
AUTHORS: NanicLews
[DESCRIPTION]
Hazel Hills CBD Gummies its 100% effective & beneficial for reducing stress, pain & anxiety {buy now on official website}
[STEPS] | [] |
88,501 | Mouse Brain Perfusion and Flash Freezing | 1 | dx.doi.org/10.17504/protocols.io.j8nlkodr6v5r/v1 | https://www.protocols.io/view/mouse-brain-perfusion-and-flash-freezing-c2nvyde6 | Allen Institute | TITLE: Mouse Brain Perfusion and Flash Freezing
AUTHORS: Allen Institute
[DESCRIPTION]
This protocol is used for transcardial perfusion with HEPES-Sucrose Cutting Solution (Referred as HEPES hereafter), followed by fast brain dissection and flash freezing.
[STEPS] | [] |
46,903 | updated version- Lake biofilms sampling for both downstream DNA analysis and microscopic counts | 1 | dx.doi.org/10.17504/protocols.io.br2xm8fn | https://www.protocols.io/view/updated-version-lake-biofilms-sampling-for-both-do-br2xm8fn | Frederic Rimet, Rainer Kurmayer, Nico Salmaso, Camilla Capelli, Cecile Chardon, Marine Vautier, Julie Gueguen, Agnès Bouchez, Isabelle Domaizon | TITLE: updated version- Lake biofilms sampling for both downstream DNA analysis and microscopic counts
AUTHORS: Frederic Rimet, Rainer Kurmayer, Nico Salmaso, Camilla Capelli, Cecile Chardon, Marine Vautier, Julie Gueguen, Agnès Bouchez, Isabelle Domaizon
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-b... | ["[When & where to sample]\nChoice of the sampling season and period- Phytobenthic communities’ composition are changing along seasons. In large lakes, the major variables explaining these temporal changes are nutrients (and phosphorus especially).- Moreover, the heterogeneity between the communities present alon... |
48,815 | Lignin and Optional Sugars Analysis for Woodchips | 1 | null | https://www.protocols.io/view/lignin-and-optional-sugars-analysis-for-woodchips-btwpnpdn | Feyereisen, Klasson | TITLE: Lignin and Optional Sugars Analysis for Woodchips
AUTHORS: Feyereisen, Klasson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Lignin and Optional Sugars Analysis for Woodchips</div><div class = "text-block">SOP 005-S-GF-19</div></div>
[STEPS]
?. Prepare washed filter papers (VWR part # 2829... | ["Prepare washed filter papers (VWR part # 28297-984)", "Rinse the filter papers on a vacuum apparatus by flushing 3x with a 20 mL aliquot of nano-pure water", "Place the rinsed filter paper onto an aluminum tray, up to 20 filter papers may be places onto one tray", "Put the aluminum tray with rinsed filter papers into... |
80,229 | CAMbank: SST Field Processing v1 | 4 | null | https://www.protocols.io/view/cambank-sst-field-processing-v1-cskdwcs6 | Eliah G Overbey, krr, jak, chm2042 | TITLE: CAMbank: SST Field Processing v1
AUTHORS: Eliah G Overbey, krr, jak, chm2042
[DESCRIPTION]
Field processing of SST vacutainers for the Cornell Aerospace Medicine Biobank (CAMbank).
Instructions for preserving: Serum and RBC Pellets.
[STEPS]
SECTION: Perform Venipuncture
1. After venipuncture, invert the tubes... | ["[Perform Venipuncture] After venipuncture, invert the tubes gently 8 to 10 times to fully mix tube anticoagulant with blood sample.\n\nStore the tube upright at room temperature until centrifugation.\n\nAllow the blood to clot in an upright position for at least 30 minutes. \nFor optimal biochemical results, centrifu... |
95,547 | Extraction, derivatisation and GC-MS/MS analysis of d2-glucose and d5-glycerol from human plasma | 1 | null | https://www.protocols.io/view/extraction-derivatisation-and-gc-ms-ms-analysis-of-c9i3z4gn | Scott G Denham, Joanna P Simpson, Natalie ZM Homer | TITLE: Extraction, derivatisation and GC-MS/MS analysis of d2-glucose and d5-glycerol from human plasma
AUTHORS: Scott G Denham, Joanna P Simpson, Natalie ZM Homer
[DESCRIPTION]
Tracer glucose and tracer glycerol is used to assess glucose kinetics and turnover.
Here we have improved upon existing gas chromatography m... | ["[Glucose and Glycerol and tracer acetate derivative analysis by GC-MS/MS] Set up an acquisition batch in Xcalibur software using the electronic Microsoft Excel file of the calibration standards and sample list. Set to inject 2 µL per sample and use a method of chromatographic separation as described in step 10 and ma... |
85,320 | Measure chlorophyll-a and pheophytin-a by Turner Designs | 4 | null | https://www.protocols.io/view/measure-chlorophyll-a-and-pheophytin-a-by-turner-d-cxjgxkjw | Ying-Yu Hu | TITLE: Measure chlorophyll-a and pheophytin-a by Turner Designs
AUTHORS: Ying-Yu Hu
[DESCRIPTION]
Here we describe a protocol for measuring chlorophyll-a and pheophytin-a from microalgae by using Turner Designs (10-AU)
[GUIDELINES]
The entire procedure should be carried out as much as possible in subdued light (Green... | ["[Prepare Chlorophyll-a standard] Primary stock: ≅10 mg/L", "[Prepare reagent] Saturated magnesium carbonate solution", "[Extract chlorophyll sample and blank] Extract samples on the filter", "[Daily calibrate] Allow Turner to warm-up for at least 15 minutes.", "[Prepare reagent] 90% buffered acetone", "[Prepare reage... |
74,732 | PROCEDIMIENTO DE GENERACIÓN INFORME ANUAL REVISIÓN GARANTÍA DE CALIDAD | 1 | dx.doi.org/10.17504/protocols.io.kxygx978dg8j/v1 | https://www.protocols.io/view/procedimiento-de-generaci-n-informe-anual-revisi-n-ck8kuzuw | cgarcia | TITLE: PROCEDIMIENTO DE GENERACIÓN INFORME ANUAL REVISIÓN GARANTÍA DE CALIDAD
AUTHORS: cgarcia
[DESCRIPTION]
Desde la EIDUCAM, es necesario establecer mecanismos de control que permitan a las Comisiones Académicas revisar diferentes aspectos relacionados con el desarrollo de nuestros Programas de Doctorado.
El inform... | ["[PROCEDIMIENTO DE GENERACIÓN INFORME ANUAL REVISIÓN GARANTÍA DE CALIDAD] Actualización WEB y Portal de Doctorando\nSe deberán revisar los contenidos públicos de la web y del portal de doctorando, indicando los aspectos a modificar y desarrollando nuevos contenidos en caso necesarios. Este apartado contemplará la actu... |
92,942 | Amplicon Sequencing for Genotyping S. Typhi | 4 | dx.doi.org/10.17504/protocols.io.36wgq31dylk5/v1 | https://www.protocols.io/view/amplicon-sequencing-for-genotyping-s-typhi-c6znzf5e | Jaspreet Mahindroo, Catherine Troman, Nick Grassly | TITLE: Amplicon Sequencing for Genotyping S. Typhi
AUTHORS: Jaspreet Mahindroo, Catherine Troman, Nick Grassly
[DESCRIPTION]
The following protocol is for amplifying and sequencing amplicons targeting Salmonella Typhi. It is primarily for use with samples that are already suspected to be positive for S. Typhi and has ... | ["[Preparation for sequencing using ONT Native barcodes] From the ONT kit (SQK-NBD114.24 or .96) thaw AMPure XP beads (AXP), mix by vortexing, then keep at room temperature.\n\nThaw the NEBnext Ultra II End Repair reagents on ice, flick or invert the tubes to mix, then spin down.", "[Preparation for sequencing using ON... |
null | null | null | dx.doi.org/10.17504/protocols.io.m6yc9fw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
81,150 | Técnica de inmunofluorescencia indirecta para detección de dengue virus | 1 | dx.doi.org/10.17504/protocols.io.rm7vzbpzxvx1/v1 | https://www.protocols.click/view/t-cnica-de-inmunofluorescencia-indirecta-para-dete-ctg6wjze | Delia Piedad Recalde-Reyes, Juliana Lopez Calderon | TITLE: Técnica de inmunofluorescencia indirecta para detección de dengue virus
AUTHORS: Delia Piedad Recalde-Reyes, Juliana Lopez Calderon
[DESCRIPTION]
La inmunofluorescencia indirecta (IFI) es una técnica para detectar la presencia de anticuerpos en una muestra. Esta se debe a la unión de anticuerpos fluorescentes a... | ["Mantenimiento línea celular BHK\n\nPara obtener celulas BHK, se requiere", "Se emplea una caja de 24 pozos para trabajar con células BHK (RPMI), se usará vidrio para cada uno de los pozos.", "Se adiciona 100.000 células por cada uno de los pozos.", "Infección viral de células eucariotas \n\nSe realiza la infección co... |
87,401 | Sanger sequencing of SARS-CoV-2 Spike protein | 4 | dx.doi.org/10.17504/protocols.io.5qpvoyzkbg4o/v3 | https://www.protocols.io/view/sanger-sequencing-of-sars-cov-2-spike-protein-czkhx4t6 | Tiago S. Salles*, Andrea Cony Cavalcanti*, Fabio Burack da Costa, Renata Campos Azevedo | TITLE: Sanger sequencing of SARS-CoV-2 Spike protein
AUTHORS: Tiago S. Salles*, Andrea Cony Cavalcanti*, Fabio Burack da Costa, Renata Campos Azevedo
[DESCRIPTION]
The SARS-CoV-2 responsible for the ongoing COVID pandemic reveals particular evolutionary dynamics and an extensive polymorphism, mainly in Spike protein. ... | ["RT-PCR\n\nProgram the thermal cycler before setting up the reaction. The thermal cycler should be preheated to 45–60°C.\nKeep all components, reaction mixes, and samples on ice. After preparation of the samples, transfer them to the preheated thermal cycler and immediately start the RT–PCR program.\nReaction mix shou... |
68,252 | Ligation | 4 | null | https://www.protocols.io/view/ligation-cev4te8w | Brian Teague | TITLE: Ligation
AUTHORS: Brian Teague
[DESCRIPTION]
A DNA ligase is an enzyme that forms phosphodiester bonds -- it can "glue" together two pieces of DNA. The ligase we're using comes from the T4 bacteriophage virus.
Why do we need to do a ligation anyway? Remember, we're going to use a Cas9 protein to "cut" your ... | ["[Ligation] Dilute the annealed oligonucleotides in to make 100 µL of working stock at a final concentration of", "[Ligation] In the PCR tube, mix in order:\n4 µL \n2 µL of the L2-01 DNA plasmid backbone\n 2 µL of the diluted annealed oligos\n1 µL \n1 µL", "[Ligation] Flick the tube several times to mix the com... |
15,617 | Assessment of antimicrobial activity | null | dx.doi.org/10.17504/protocols.io.tg9ejz6 | null | Elizabeth Ortiz-Vázquez, Jesus Manuel Ramon Sierra | TITLE: Assessment of antimicrobial activity
AUTHORS: Elizabeth Ortiz-Vázquez, Jesus Manuel Ramon Sierra
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The indiscriminate use of antibiotics is one of the main factors that cause the emergence of resistant microorganisms, and it has become a global he... | ["Inoculum preparationThe microorganisms were inoculated in LB-Agar medium and incubated at 37 ° C from 12 to 24 hours.", "Three colonies of the strains previously incubated were dissolved in 990 µL of saline solution (0.85% of NaCl).", "The microbial suspension was adjusted to a concentration of 1X108 CFU equivalent t... |
24,133 | General preparation of liposomes using probe-tip sonication | null | dx.doi.org/10.17504/protocols.io.3tdgni6 | null | Monica Rieth, Andrew Lozano, Jordan Grant | TITLE: General preparation of liposomes using probe-tip sonication
AUTHORS: Monica Rieth, Andrew Lozano, Jordan Grant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This method outlines a general approach for preparing liposomes using probe-tip sonication. The method has been optimized for the pre... | ["Lipids are typically stored at and should be allowed to reach room temperature prior to working with them.For DPPC powder, carefully weigh out 25 mg on a clean analytical balance using clean, ungloved hands (to minimize static)\n-20 °C", "Carefully transfer the lipid to a clean 2.0 mL glass vial. Add 2.0 mL of (0.... |
70,684 | SOP Template | 1 | null | https://www.protocols.io/view/sop-template-cg94tz8w | Catherine.Cook | TITLE: SOP Template
AUTHORS: Catherine.Cook
[DESCRIPTION]
Study Title: Rapid Research in Diagnostics Development for TB Network (R2D2 TB Network) Study
SOP Title: Template
SOP ID:
Division: Data, Lab,
Effective Date:
Written by:
[STEPS]
SECTION: Purpose
1. This document describes in detail the procedure for locati... | ["[Purpose] This document describes in detail the procedure for locating and measuring MUAC (Middle Upper Arm Circumference) during the baseline questionnaire, Follow-up visit 1 , and Follow-up visit 2 for R2D2 study participants", "[Definitions] REDCap: Research Electronic Data Capture, a secure, online database that... |
47,412 | CSF Processing | 4 | dx.doi.org/10.17504/protocols.io.yxmvmkpe5g3p/v1 | https://www.protocols.io/view/csf-processing-bsiuncew | Clemens Scherzer, Bradley Hyman, Charles Jennings | TITLE: CSF Processing
AUTHORS: Clemens Scherzer, Bradley Hyman, Charles Jennings
[DESCRIPTION]
This protocol explains the Standard Operating Protocol for processing CSF.
[GUIDELINES]
FREEZER STORAGE
Freezers are divided into 4 shelves, with 6 racks per shelf, and 24 boxes that can be held in each shelf. In total, ... | ["[CSF Processing] On dry ice, scan and position aliquots into the Freezerworks Inventory Program.", "[CSF Processing] Stores aliquots in -80 °C freezer with 2 hours of LP draw."] |
96,767 | WATER PRODUCTION FOR AQUAPONICS | 0 | dx.doi.org/10.17504/protocols.io.4r3l22xxxl1y/v1 | https://www.protocols.io/view/water-production-for-aquaponics-daq72dzn | Celia Manaia | TITLE: WATER PRODUCTION FOR AQUAPONICS
AUTHORS: Celia Manaia
[DESCRIPTION]
The protocol summarises the procedures used for analytical control – detailed protocols are annexed to this protocol.
[GUIDELINES]
RECOMMENDED/ACCEPTED VALUE
#Step2.1: Mesophilic Bacteria in PCA (Plate Count Agar):
According to drinking water... | ["[Methods: The section below summarises the procedures used for analytical control – detailed protocols are annexed to this protocol.] Mesophilic Bacteria in PCA (Plate Count Agar):", "[Methods: The section below summarises the procedures used for analytical control – detailed protocols are annexed to this protocol.] ... |
62,106 | Fun Drops CBD Gummies Male Enhancement - Removes Your Issues That Hinder Your Pride In The Bed - Increased Sexual Drive | 3 | dx.doi.org/10.17504/protocols.io.261genxb7g47/v1 | https://www.protocols.io/view/fun-drops-cbd-gummies-male-enhancement-removes-you-b8v2rw8e | Fun Drops CBD Gummies | TITLE: Fun Drops CBD Gummies Male Enhancement - Removes Your Issues That Hinder Your Pride In The Bed - Increased Sexual Drive
AUTHORS: Fun Drops CBD Gummies
[DESCRIPTION]
Fun Drops CBD Gummies
[STEPS] | [] |
97,100 | USDA LTAR Common Experiment measurement: Concentration of carbon and nitrogen in aboveground biomass | 1 | dx.doi.org/10.17504/protocols.io.bp2l62km5gqe/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-concentrat-da3k2gkw | Michel A. Cavigelli, Timothy C. Strickland | TITLE: USDA LTAR Common Experiment measurement: Concentration of carbon and nitrogen in aboveground biomass
AUTHORS: Michel A. Cavigelli, Timothy C. Strickland
[DESCRIPTION]
The carbon (C) and nitrogen (N) contents within a plant are determined from biomass samples collected following the USDA LTAR Common Experiment m... | ["[Sample collection, processing, and analysis] Collect plant biomass samples as per the USDA LTAR Common Experiment measurement: Aboveground biomass (Wilke et al., 2024) protocol. Conduct C and N analyses on a ground subsample of a size based on individual instruments and/or the N concentration of the material.", "[Sa... |
59,593 | Processing frozen human blood samples for population-scale Oxford Nanopore long-read DNA sequencing SOP | 1 | dx.doi.org/10.17504/protocols.io.ewov1n93ygr2/v1 | https://www.protocols.io/view/processing-frozen-human-blood-samples-for-populati-b6fhrbj6 | Kimberley J Billingsley, Pilar Alvarez Jerez, Abigail Miano-Burkhardt, Cornelis Blauwendraat, on behalf of the CARD Long-read Team | TITLE: Processing frozen human blood samples for population-scale Oxford Nanopore long-read DNA sequencing SOP
AUTHORS: Kimberley J Billingsley, Pilar Alvarez Jerez, Abigail Miano-Burkhardt, Cornelis Blauwendraat, on behalf of the CARD Long-read Team
[DESCRIPTION]
Abstract:
As part of the GP2 initiative we will genera... | ["Part 1: Preparing Blood Samples (~20 mins for 8 samples)", "Obtain blood samples from -80C freezer and thaw at 37 °C for 15 min\n\n Note: This is specifically for 6mL tubes, if starting with only 1mL thaw until warm (~ 5 min)", "Inversion mix blood x10.\n\nNote: The blood sample needs to be very thoroughly mixed in... |
32,428 | Low Quantity single strand CAGE protocol | null | dx.doi.org/10.17504/protocols.io.bbwkipcw | null | Hazuki Takahashi, Hiromi Nishiyori-Sueki, Piero Carninci | TITLE: Low Quantity single strand CAGE protocol
AUTHORS: Hazuki Takahashi, Hiromi Nishiyori-Sueki, Piero Carninci
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The </span><span style = ":UNDERLINE;">C</span><span>ap </span><span style = ":UNDERLINE;">A</span><span>nalysis of </span><span sty... | ["Reverse Transcription (RT) to generate RNA/cDNA hybrids", "Oxidation to modify diol group of cap structurea. Mix 40 µL of RNA/cDNA hybrid from step 1.1e. or 1.2g., 2 µL of 1 M NaOAc (pH 4.5) and 2 µL of 250 mM NaIO4 in a tube by 10 times pipetting.b. Incubate for 5 min on ice in dark by aluminum foil wrapping.c. Add ... |
73,686 | Implant Surgery: Chronic recoverable Neuropixels in mice | 1 | null | https://www.protocols.io/view/implant-surgery-chronic-recoverable-neuropixels-in-cj7wurpe | Emily A Aery Jones | TITLE: Implant Surgery: Chronic recoverable Neuropixels in mice
AUTHORS: Emily A Aery Jones
[DESCRIPTION]
This protocol collection explains how to build a low-cost, lightweight system to implant Neuropixels 1.0 probes into mice, record during freely moving behavior, then recover the probe for future use. This protocol... | ["[Prepare surgical tools and field] Sterilize tips of metal instruments, ground screw, and headbar in autoclave 25 min or hot bead sterilizer 5 s . Disinfect cotton swabs, toothpick, and a weigh boat under UV light 30 min .", "[Prepare surgical tools and field] Disinfect surgical field with 10% bleach. Lay absorbent ... |
76,802 | Surgical window preparation to visualize the trigeminal ganglion in an anesthetized mouse | 4 | null | https://www.protocols.io/view/surgical-window-preparation-to-visualize-the-trige-cn9avh2e | Elizabeth A. Ronan, Deanna N Cannizzaro, Joshua J. Emrick | TITLE: Surgical window preparation to visualize the trigeminal ganglion in an anesthetized mouse
AUTHORS: Elizabeth A. Ronan, Deanna N Cannizzaro, Joshua J. Emrick
[DESCRIPTION]
The trigeminal ganglion houses somatosensory neurons that innervate peripheral structures throughout the head and face. This protocol describ... | ["[Positioning the mouse in the stereotax] Induce anesthesia using isofluorane according to your approved animal protocol.", "[Bilateral Hemispherectomy and Exposure of Trigeminal Ganglia] Hold skin up with curved forceps. Cut and remove skin above the skull and carefully remove overlying connective tissue (scalp).", "... |
44,358 | OnsiteGene 4 Protocol Saliva Extract | 4 | dx.doi.org/10.17504/protocols.io.bpjemkje | https://www.protocols.io/view/onsitegene-4-protocol-saliva-extract-bpjemkje | yliu | TITLE: OnsiteGene 4 Protocol Saliva Extract
AUTHORS: yliu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This OnsiteGene protocol is designed for testing the normal saliva samples with nucleic acid extraction. A normal saliva collection tube is used for the collection. The protocol uses the... | ["[Sample Collection Procedure]\nSaliva sample should be collected with the assistance of a healthcare worker or technician.", "[Sample Collection Procedure]\nBefore collection, clean hands using alcohol-based sanitizer or soap and water (no fragrances) and wear appropriate PPE (at minimum, gloves and a mask).", "[Samp... |
null | null | null | dx.doi.org/10.17504/protocols.io.efxbbpn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol provides a method for detecting Human Polyomaviruses (HPyVs) in our virome and whole metagenome datasets. For genome reference, download the HPyV genomes (in fasta format) from NCBI (nucleotide) using the search terms "Human Polyomavirus" AND "Complete Genome". Thi... | [] |
61,978 | Publication Data Cleaning in Excel for Author Name Gender Analysis | 1 | dx.doi.org/10.17504/protocols.io.261genxjwg47/v1 | https://www.protocols.io/view/publication-data-cleaning-in-excel-for-author-name-b8r2rv8e | Stephen W Gabrielson, Rose L Turner | TITLE: Publication Data Cleaning in Excel for Author Name Gender Analysis
AUTHORS: Stephen W Gabrielson, Rose L Turner
[DESCRIPTION]
This protocol provides step-by-step instructions for downloading publication data from Dimensions, cleaning the data to identify first and last authors, and uploading the data into the ... | ["[Getting Publication Data] From the Dimensions homepage, click on Access Free Web App.", "[Getting Publication Data] To limit the results to a certain journal on the Free Web App landing page, expand the Source Title filter in the left column. Click More at the bottom, type in the full name of the journal in the sear... |
88,880 | Yale University_Spatial ATAC Sequencing for Fixed Fresh Frozen Human Lymph node Tissue via DBiT-seq | 1 | dx.doi.org/10.17504/protocols.io.kxygx3nywg8j/v1 | https://www.protocols.io/view/yale-university-spatial-atac-sequencing-for-fixed-c22qygdw | Negin Farzad, Yanxiang Deng, Archibald Enninful, yao.lu.yl, Rong Fan | TITLE: Yale University_Spatial ATAC Sequencing for Fixed Fresh Frozen Human Lymph node Tissue via DBiT-seq
AUTHORS: Negin Farzad, Yanxiang Deng, Archibald Enninful, yao.lu.yl, Rong Fan
[DESCRIPTION]
This protocol describes the use of Deterministic Barcoding in Tissue for spatial ATAC sequencing to construct an epigeno... | ["[Fabricating the Silicon Wafer Device Mold] Prepare a high-resolution computer-aided-design (CAD) file with the desired microfluidics chip design. A CAD file is also available in the link provided. (https://ars.els-cdn.com/content/image/1-s2.0-S2666166721002392-mmc4.zip)", "[Fabricating the Silicon Wafer Device Mold]... |
86,085 | Inducing proteostasis stress | 1 | null | https://www.protocols.io/view/inducing-proteostasis-stress-cybdxsi6 | Louise Uoselis | TITLE: Inducing proteostasis stress
AUTHORS: Louise Uoselis
[DESCRIPTION]
Inducing proteostasis stress in HeLa cells with G-TPP to analyse proteostasis protection and repair.
[STEPS]
SECTION: Day 1
1. Seed cells in standard growth media, aiming for a density of ~80-90% the following day.
SECTION: Day 3
4. Harvest th... | ["[Day 1] Seed cells in standard growth media, aiming for a density of ~80-90% the following day.", "[Day 3] Harvest the G-TPP treated samples and DMSO control samples after the desired incubation period (eg. 2 – 12 h).", "[Day 2] Feed cells for 1 h prior to starting treatment by aspirating the media from each well and... |
55,236 | NCBI submission protocol for microbial pathogen surveillance | 1 | dx.doi.org/10.17504/protocols.io.bz7cp9iw | https://www.protocols.io/view/ncbi-submission-protocol-for-microbial-pathogen-su-bz7cp9iw | Ruth Timme, Maria Balkey, Robyn Randolph, Julie Haendiges, Sai Laxmi Gubbala Venkata, William Wolfgang, Errol Strain | TITLE: NCBI submission protocol for microbial pathogen surveillance
AUTHORS: Ruth Timme, Maria Balkey, Robyn Randolph, Julie Haendiges, Sai Laxmi Gubbala Venkata, William Wolfgang, Errol Strain
[DESCRIPTION]
PURPOSE: Step-by-step instructions for submitting pathogen whole genome sequence data to NCBI and to the... | ["[Establish submission environmnet at NCBI] Set up a new NCBI submission environment for your lab:\n\n1.1: Create an NCBI user account\n1.2: Set up an NCBI submission user group for your lab\n1.4: Bookmark the link to your submission portal\n1.5. Identify or establish new BioProjects (detailed in Step 3)\n\n\nReady fo... |
81,628 | TS Spurrs - unstained FFPE on Thermanox coverslips (TM - 013) | 4 | dx.doi.org/10.17504/protocols.io.3byl4b7rjvo5/v4 | https://www.protocols.io/view/ts-spurrs-unstained-ffpe-on-thermanox-coverslips-t-ctx4wpqw | sandra.crameri | TITLE: TS Spurrs - unstained FFPE on Thermanox coverslips (TM - 013)
AUTHORS: sandra.crameri
[DESCRIPTION]
Processing unstained FFPE sections on Thermanox coverslips to Spurrs resin block for EM.
[GUIDELINES]
All step times are the minimum time required. Longer steps are acceptable.
[STEPS]
SECTION: HEADER
1. SAN:
... | ["[HEADER] SAN:\n\n\nSPEC No:\n\n\nOPERATOR & STEPS:\n\n\nOPERATOR & STEPS:", "[Dewax FFPE sections] or X3B 5 min", "[Dewax FFPE sections] or X3B 5 min", "or X3B 5 min", "[Rehydrate] 100 % volume 5 min", "95 % volume 5 min", "70 % volume 5 min", "[Conventional - with reduced times for 5um thick FFPE section] 2.5... |
22,343 | Vibrio natriegens electrocompetent preparation | null | dx.doi.org/10.17504/protocols.io.z3ff8jn | null | David Shis, Weinstock MT, Hesek ED, Wilson CM, Gibson DG | TITLE: Vibrio natriegens electrocompetent preparation
AUTHORS: David Shis, Weinstock MT, Hesek ED, Wilson CM, Gibson DG
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is an approach to electroporate DNA into Vibrio natriegens wt and Vibrio natrigiens sp. Vmax. It's generally pretty similar to ... | ["[Media recipes]\nBHI media stock", "[Media recipes]\nBHI + v2 salts- NaCl- KCl- MgCl2- SucroseFilter sterilize or autoclave\n204 Milimolar (mM)\n4 Milimolar (mM)\n23 Milimolar (mM)\n680 Milimolar (mM)", "[Electrocompetent prep]\n**** The day before****Inoculate 10 mL [BHI + v2 salts] with V. natriegens overnight cult... |
19,220 | iDISCO protocol for whole-mount immunostaining and volume imaging | 1 | dx.doi.org/10.17504/protocols.io.wzuff6w | https://www.protocols.io/view/idisco-protocol-for-whole-mount-immunostaining-and-wzuff6w | Clara Huesing, Heike Muenzberg, David Burk, Hayden Torres | TITLE: iDISCO protocol for whole-mount immunostaining and volume imaging
AUTHORS: Clara Huesing, Heike Muenzberg, David Burk, Hayden Torres
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol documents how to dehydrate, immunolabel, and clear tissue in preparation for light sheet microscopy.</d... | ["[Methanol Pretreatment]\na. Clean workspaceb. Dehydrate samples with a series of MeOH/H2O washes: 20% MeOH/ 80% H2O 40% MeOH/ 60% H2O 60% MeOH/ 40% H2O 80% MeOH/ 20% H2O 100% MeOH 100% MeOHeach wash. Place samples on rocker at room temperature during washes. *Before continui... |
61,554 | A versatile nuclei extraction protocol for single nucleus sequencing in fish species – optimization in various Atlantic salmon tissues. | 4 | dx.doi.org/10.17504/protocols.io.261genwm7g47/v1 | https://www.protocols.io/view/a-versatile-nuclei-extraction-protocol-for-single-b8csrswe | Rose Ruiz Daniels, Richard S Taylor, Ross Dobie, Sarah Salisbury, Emily Clark, Dan Macqueen, Diego Robledo | TITLE: A versatile nuclei extraction protocol for single nucleus sequencing in fish species – optimization in various Atlantic salmon tissues.
AUTHORS: Rose Ruiz Daniels, Richard S Taylor, Ross Dobie, Sarah Salisbury, Emily Clark, Dan Macqueen, Diego Robledo
[DESCRIPTION]
Single cell RNA sequencing has rapidly become... | ["[Nucleus isolation workflow for ST-based buffers] on ice, mince tissue initially using Tungsten Carbide scissors for 30 s and then with Noyes Spring Scissors for a total of 10 min.", "[Nucleus isolation workflow for ST-based buffers] Pass lysate through a cell strainer .", "[Nucleus isolation workflow for ST-base... |
21,580 | Dissolved inorganic carbon concentration and 13C/12C | null | dx.doi.org/10.17504/protocols.io.zbkf2kw | null | Daniel Nothaft | TITLE: Dissolved inorganic carbon concentration and 13C/12C
AUTHORS: Daniel Nothaft
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>CO</span><span style = "vertical-align:sub;">2</span><span> conversion by H</span><span style = "vertical-align:sub;">3</span><span>PO</span><span style = "vertic... | ["[Laboratory preparation]\nPlan samplesBefore prepping vials, you must consider what range of dissolved inorganic carbon (DIC) you expect in your samples.The sensitivity range is determined by the method blank, the water volume, the lower limit of mass for which we can consistently measure carbonate standards (~10 µg)... |
71,934 | Glycolysis stress test in organoids | 4 | null | https://www.protocols.io/view/glycolysis-stress-test-in-organoids-cig6ubze | gustavo.parfitt | TITLE: Glycolysis stress test in organoids
AUTHORS: gustavo.parfitt
[DESCRIPTION]
Glycolysis stress test in organoids
[STEPS]
1. were coated with Laminin solution in L15+Bicarbonate: final concentration of 10 μg/mL .
2. Day 35 midbrain organoids were placed in the plates and media was changed every other day.
3. ... | ["were coated with Laminin solution in L15+Bicarbonate: final concentration of 10 μg/mL .", "Day 35 midbrain organoids were placed in the plates and media was changed every other day.", "On the day of the assay supplemented with B27 + N2 was warmed at 37 °C and the pH as adjusted to 7.4. And 175 ul was added to the... |
68,612 | Tissue Dissociation for Multiome Analysis Using S2 Singulator | 4 | dx.doi.org/10.17504/protocols.io.yxmvmndx6g3p/v1 | https://www.protocols.io/view/tissue-dissociation-for-multiome-analysis-using-s2-ce9cth2w | Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill | TITLE: Tissue Dissociation for Multiome Analysis Using S2 Singulator
AUTHORS: Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill
[DESCRIPTION]
This protocol describes dissociation of snap-frozen tissue in order to iso... | ["Pre-chill nuclei cartridge in the fridge overnight. \n\nTip: Keep cartridge at 4 °C until ready for use.", "Turn the Singulator 100 on by turning on the main machine, tablet, then cooler chamber (in that order).", "Place the Nuclei Isolation Reagent (NIR) and Nuclei Storage Reagent (NSR) in the cooler and connect th... |
102,542 | IMMUNOCYTOCHEMISTRY PROTOCOL | 0 | dx.doi.org/10.17504/protocols.io.36wgqnorygk5/v1 | https://www.protocols.io/view/immunocytochemistry-protocol-dgdn3s5e | Scott Vermilyea | TITLE: IMMUNOCYTOCHEMISTRY PROTOCOL
AUTHORS: Scott Vermilyea
[DESCRIPTION]
This protocol details about the Immunocytochemistry.
[STEPS]
SECTION: Procedure
1. Select coverslips previously fixed in 4% paraformaldehyde and subsequently washed and stored in PBS solution.
SECTION: Procedure
2. Permeabilize: 0.2% TX-100 ... | ["[Procedure] Select coverslips previously fixed in 4% paraformaldehyde and subsequently washed and stored in PBS solution.", "[Procedure] Permeabilize: 0.2% TX-100 in PBS for 15 min at Room temperature .", "[Procedure] Mount coverslips to slide with Vectashield antifade mounting medium.", "[Procedure] Wash 3 x 5 min i... |
null | null | null | dx.doi.org/10.17504/protocols.io.gvbbw2n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p>The In-Cell Western assay is a popular immunoassay for the study of signal transduction, protein expression, and function. A key feature in this assay is its ability to simultaneously measure two ... | ["[Cell Preparation and Fixation] {\"blocks\":[{\"key\":\"6vm3p\",\"text\":\"Prepare fresh Fixing Solution as follows:\",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[{\"offset\":14,\"length\":15,\"style\":\"italic\"},{\"offset\":14,\"length\":15,\"style\":\"bold\"}],\"entityRanges\":[],\"data\":[]},{\"key\"... |
97,401 | 3D printed stereotax multimodal imaging compatibility assessment | 0 | dx.doi.org/10.17504/protocols.io.j8nlk85edl5r/v1 | https://www.protocols.io/view/3d-printed-stereotax-multimodal-imaging-compatibil-dbcz2ix6 | Isabela Zimmermann Rollin, Lucy Liang, David J Schaeffer | TITLE: 3D printed stereotax multimodal imaging compatibility assessment
AUTHORS: Isabela Zimmermann Rollin, Lucy Liang, David J Schaeffer
[DESCRIPTION]
PET/CT and MRI for multimodal image compatibility.
This protocol is supplementary to the manuscript:
Liang, L., Zimmermann Rollin, I., Alikaya, A., Ho, J.C., Santini... | ["[PET/CT and MRI for multimodal image compatibility] Preparation of eye bars and ear bars: With a syringe, fill the ear and eye bars with a small dose (0.05 MBq ear bar, 1.54 MBq eye bar) of [18F] fluorodeoxyglucose (FDG) and seal the chamber with a MRI-compatible nylon screw. Note that these doses are for proof of co... |
null | null | null | dx.doi.org/10.17504/protocols.io.n7jdhkn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol was designed and developed at this laboratory.</p>
<p>The assay targets the capsid peptide coding region of DENV 1-4 and is desigend as a qualitative screening test for human cases of DENV infection.</p>
[BEFORE_START]
<div>
<ul>
<li>If using a different brand ... | [] |
62,428 | Weight Crasher Keto Gummies : How Does it Works? | 1 | dx.doi.org/10.17504/protocols.io.5jyl896e9v2w/v1 | https://www.protocols.io/view/weight-crasher-keto-gummies-how-does-it-works-b874rzqw | sinkdot | TITLE: Weight Crasher Keto Gummies : How Does it Works?
AUTHORS: sinkdot
[DESCRIPTION]
Weight loss is not as hard as we think. With a proper diet or supplements, we can easily attain a slim and toned body. Exercising, and dieting may not offer instant results, but supplements like Weight Crasher Keto Gummies offer r... | ["[Weight Crasher Keto Gummies]"] |
69,844 | Salicornia Germination on Agar Plate | 1 | dx.doi.org/10.17504/protocols.io.kqdg394xpg25/v1 | https://www.protocols.io/view/salicornia-germination-on-agar-plate-cgfuttnw | Lina Maria Caceres Leal | TITLE: Salicornia Germination on Agar Plate
AUTHORS: Lina Maria Caceres Leal
[DESCRIPTION]
This method describes the steps to germinate seeds of various Salicornia species under sterile conditions for different purposes; germination of wild-collected seeds, tissue culture and where disease/pest-free plants are require... | ["[Media preparation] Salicornia medium germination. \nOne liter of medium is enough for approximately 20 Petri dishes (100mm x 100mm x 15mm).", "[Media preparation] Store the Petri dishes at room temperature for short-term storage or at 4 °C for long-term storage.", "[Sterilization of seeds] Place the seeds in a 2 mL ... |
94,534 | Aggregation of human recombinant alpha-synuclein | 6 | dx.doi.org/10.17504/protocols.io.6qpvr35obvmk/v1 | https://www.protocols.io/view/aggregation-of-human-recombinant-alpha-synuclein-c8jezuje | Minee-Liane Choi | TITLE: Aggregation of human recombinant alpha-synuclein
AUTHORS: Minee-Liane Choi
[DESCRIPTION]
This protocol describes a method of generating aggregation of human recombinant alpha-synuclein.
[STEPS]
1. Humanrecombinant α-Syn Monomeric WTα-Syn is purified from Escherichia coli as
previously described in
[Hoyer e... | ["Humanrecombinant α-Syn Monomeric WTα-Syn is purified from Escherichia coli as\npreviously described in \n \n[Hoyer et al., 2002].", "Aggregation reactions are carried out using a solution of α-Syn 70 micromolar (µM) in 25 millimolar (mM) Tris buffervsupplemented with 100 millimolar (mM) NaCl, pH 7.4 (in the presence... |
53,111 | Longmire lysis buffer | 6 | dx.doi.org/10.17504/protocols.io.bx4xpqxn | https://www.protocols.io/view/longmire-lysis-buffer-bx4xpqxn | Abigail Wells, Linda Park | TITLE: Longmire lysis buffer
AUTHORS: Abigail Wells, Linda Park
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Lysis buffer recipe (Longmire et al 1997): </div><div class = "text-block">To make 1 liter</div></div>
[STEPS]
?. [To make 1 L of Longmire]
975 ml double-distilled water
?. [To make 1 L o... | ["[To make 1 L of Longmire]\n975 ml double-distilled water", "[To make 1 L of Longmire]\n100 ml of 1 M Tris-HCL, pH 8.0", "[To make 1 L of Longmire]\n200 ml of 0.5 M EDTA, pH8.0", "[To make 1 L of Longmire]\n2 ml of 5 M NaCl", "[To make 1 L of Longmire]\n25 ml of 20% SDS (w/v) Filter the buffer with an autofill PES bo... |
22,679 | Hydrogen cyanide determination in cassava leaves using the picrate paper method | null | dx.doi.org/10.17504/protocols.io.2dxga7n | null | Matema Imakumbili | TITLE: Hydrogen cyanide determination in cassava leaves using the picrate paper method
AUTHORS: Matema Imakumbili
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">This protocol describes how to prepare and analyse cassava leaf samples for their total h... | ["[Setting up your work area]\nA bench or table will be needed as a working surface. Place the mortar, pestle, scissors, paper napkins and various contents of the Protocol E kit on the work table. The mortar and pestle should have been thoroughly cleaned before the start of the analysis and so should have been the scis... |
77,756 | Protocol for the development of coarse-grained structures for macromolecular simulation using GROMACS | 5 | dx.doi.org/10.17504/protocols.io.kxygx92rdg8j/v1 | https://www.protocols.io/view/protocol-for-the-development-of-coarse-grained-str-cp64vrgw | M Purushotham Rao, Akshay Uttarkar, Vidya Niranjan | TITLE: Protocol for the development of coarse-grained structures for macromolecular simulation using GROMACS
AUTHORS: M Purushotham Rao, Akshay Uttarkar, Vidya Niranjan
[DESCRIPTION]
This paper presents a protocol for the development of coarse-grained (CG) structures for macromolecular simulation using the GROMACS s... | ["[DOWNLOAD NECESSARY PROTEIN] DOWNLOAD THE PDB FILE FROM https://www.rcsb.org/\nHere, in this tutorial DUSP28 https://www.rcsb.org/structure/5Y15 is used.\nPreprocess the pdb to remove all ions and B chain or can obtained from here https://drive.google.com/file/d/1YDJV2hKtZ5dJrl8S6A_4AFdTt_lVJFMv/view?usp=sharing", "[... |
null | null | null | dx.doi.org/10.17504/protocols.io.jjickke | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This week we’re going to take a tour of the server, which will be our home base for the next several weeks. There are important things to know about how to navigate the server, how to use it politely, and how to look at files. We’re mostly going to be accessing the server via... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.natdaen | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Polyol dehydrogenases are enzymes that convert polyalcohols into sugars, using NAD<sup>+</sup> as hydrogen acceptor. Sorbitol, mannitol or other polyalcohols can be used as substrates, and the activity of these enzymes is assessed by monitoring the production of NADH. This pr... | [] |
27,149 | Western Blotting | null | dx.doi.org/10.17504/protocols.io.6rmhd46 | null | Karina Conkrite | TITLE: Western Blotting
AUTHORS: Karina Conkrite
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">From growth to isolation and lysis, to quantification, and running the gel, transfer, blotting... the whole bit.</div></div>
[STEPS]
?. [Before Starting:]
Before Starting:Prepare fresh Lysis Buffer for ... | ["[Before Starting:]\nBefore Starting:Prepare fresh Lysis Buffer for cells. Use 5X stock stored at 4C. Stock solution consists of ABC1Component1X5X2Tris25 mM125 mM3NaCl150 mM750 mM4EGTA1 mM5 mM5EDTA1 mM5 mM6NaF10 mM50 mMRemaining ingredients to be added fresh to each sample:Final concentration1 mM DTT1% Triton X-1001... |
49,744 | Protocol for preparing seagrass-microbiome samples for DNA extraction | 1 | dx.doi.org/10.17504/protocols.io.butqnwmw | https://www.protocols.io/view/protocol-for-preparing-seagrass-microbiome-samples-butqnwmw | Cassie Ettinger | TITLE: Protocol for preparing seagrass-microbiome samples for DNA extraction
AUTHORS: Cassie Ettinger
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details methods for preparing seagrass-asscociated microbiome samples for DNA extraction.</div></div>
[STEPS]
?. [Weighing samples:]
Pl... | ["[Weighing samples:]\nPlace weigh boat on balance and tare.\nBefore proceeding through wash steps (if necessary), samples should be weighed (this is important if you are considering quantitative PCR or want to standardize sample amounts); prepare balance for samples by cleaning with 70% ethanol solution and obtain st... |
62,210 | Keto Max Science Reviews - How Does It Works For Weight Loss? | 1 | dx.doi.org/10.17504/protocols.io.6qpvr6rxovmk/v1 | https://www.protocols.io/view/keto-max-science-reviews-how-does-it-works-for-wei-b8zarx2e | ketosciencereviewsloss | TITLE: Keto Max Science Reviews - How Does It Works For Weight Loss?
AUTHORS: ketosciencereviewsloss
[DESCRIPTION]
Keto Max Science Reviews - How Does It Works For Weight Loss?
[STEPS]
1. Keto Max Science Reviews - How Does It Works For Weight Loss?
Keto Max Science Canada Review - Would you like weight loss to be... | ["Keto Max Science Reviews - How Does It Works For Weight Loss?\nKeto Max Science Canada Review - Would you like weight loss to be easier? Do you feel like there's no bone that works for you in your exercise or eating habits? Would you also say that you're too busy to suppose about eating healthy or working out? Keto M... |
null | null | null | dx.doi.org/10.17504/protocols.io.egbbbsn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Cleans and concentrates any DNA. Typically useful after PCR, restriction digest, or anything where DNA was altered and now you need your newly altered DNA in a pure form.
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
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