id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.nhedb3e | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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70,865 | Protocol | 1 | dx.doi.org/10.17504/protocols.io.3byl4jke8lo5/v1 | https://www.protocols.io/view/protocol-chfrt3m6 | Tea Borkowska, Nikoloz Chkhartishvili, Ekaterine Karkashadze, Otar Chokoshvili, Pati gabunia, Lali Sharvadze, Tengiz Tsertsvadze | TITLE: Protocol
AUTHORS: Tea Borkowska, Nikoloz Chkhartishvili, Ekaterine Karkashadze, Otar Chokoshvili, Pati gabunia, Lali Sharvadze, Tengiz Tsertsvadze
[DESCRIPTION]
Abstract
Background
Life expectancy and quality of life of people living with HIV have been dramatically improved after introducing antiretroviral ther... | ["Prepare database just like one in the Materials section.", "Analyse the data contained in database.", "Formulate conclusions."] |
60,154 | 2022 GenomeTrakr Proficiency Testing exercise (PulseNet Harmonized) | 1 | dx.doi.org/10.17504/protocols.io.e6nvw5m9dvmk/v1 | https://www.protocols.io/view/2022-genometrakr-proficiency-testing-exercise-puls-b6y2rfye | Maria Balkey, Ruth Timme, Julie Haendiges | TITLE: 2022 GenomeTrakr Proficiency Testing exercise (PulseNet Harmonized)
AUTHORS: Maria Balkey, Ruth Timme, Julie Haendiges
[DESCRIPTION]
This SOP outlines guidelines on how to process the isolates for the 2022 GenomeTrakr (GT) Proficiency Testing exercise.
This SOP is applicable to all GenomeTrakr labs partici... | ["[Culture Preparation] Salmonella and Escherichia/Shigella Lyophilized cultures:\n\nDay 1\n\nDocument the isolate number(s) and the lyophilized date(s) for your records. Wipe the aluminum cover and outside of the vial with isopropyl alcohol. Using sturdy forceps, aseptically remove the aluminum cover and rubber stoppe... |
98,107 | Ex vivo cell isolation | 0 | dx.doi.org/10.17504/protocols.io.q26g71b1kgwz/v1 | https://www.protocols.io/view/ex-vivo-cell-isolation-db232qgn | Dorien De Pooter, Ben De Clerck, Koen Dockx, Domenica De Santis, Sarah Sauviller, Pascale Dehertogh, Matthias Beyens, Isabelle Bergiers, Isabel Nájera, Ellen Van Gulck, Nádia Conceição-Neto, Wim Pierson | TITLE: Ex vivo cell isolation
AUTHORS: Dorien De Pooter, Ben De Clerck, Koen Dockx, Domenica De Santis, Sarah Sauviller, Pascale Dehertogh, Matthias Beyens, Isabelle Bergiers, Isabel Nájera, Ellen Van Gulck, Nádia Conceição-Neto, Wim Pierson
[DESCRIPTION]
This protocol details ex-vivo cell isolation.
[STEPS]
SECTION:... | ["[Reagent preparation:] PBS-2% FCS: Thaw an aliquot of filtered FCS and prepare a solution of 2% FCS (vol/vol) in 1x PBS.", "[Reagent preparation:] 33.75% Percoll gradient: Prepare an isotonic solution of 33.75% Percoll using 10x PBS and 1x PBS-2% FCS. Prepare this solution fresh and store atRoom temperature and prote... |
85,637 | Human urine procurement and processing | 1 | dx.doi.org/10.17504/protocols.io.dm6gp3bmjvzp/v1 | https://www.protocols.click/view/human-urine-procurement-and-processing-cxvdxn26 | hannah.anvari, asma.giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan francesca.duncan | TITLE: Human urine procurement and processing
AUTHORS: hannah.anvari, asma.giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan francesca.duncan
[DESCRIPTION]
Purpose: This protocol is intended for use in the collection and storage of human urine in a research setting. The protocol details the collection, proce... | ["A urine sample is collected in a standard specimen cup during pre-op from a nurse. Once the urine sample has been collected it is placed immediately on ice following collection. A research coordinator will bring the urine sample to the lab on ice (2-4 °C). Urine samples should be transported in a blue, biohazard labe... |
58,104 | Pluripotency markers staining | 4 | dx.doi.org/10.17504/protocols.io.b4yyqxxw | https://www.protocols.io/view/pluripotency-markers-staining-b4yyqxxw | Hanqin Li, Dirk Hockemeyer, Frank Soldner | TITLE: Pluripotency markers staining
AUTHORS: Hanqin Li, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes the standard procedure for staining pluripotency markers, e.g. OCT4, SSEA4, alkaline phosphatase, and etc. on human pluripotent stem cells (hPSCs).
Protocol overview
A. Immunofluorescence st... | ["[A. Immunofluorescence staining] Wash cells once with PBS", "[A. Immunofluorescence staining] Fix cells with 4% PFA at 4 Room temperature for 15 min", "[A. Immunofluorescence staining] Wash cells twice with PBS, incubate 5 min in between washes at Room temperature", "[A. Immunofluorescence staining] Incubate in 3% B... |
null | null | null | dx.doi.org/10.17504/protocols.io.frjbm4n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Cas9 nuclease may be used <em>in vivo</em> to create targeted genome modifications. There are several ways in which to introduce Cas9-guide RNA complexes into cells. Here we present a method for the transfection of Cas9 RNP’s into HEK293 FT cells using Thermo Fisher Lipofec... | [] |
91,481 | Imaging axonal calcium dynamics in ex vivo mouse brain slices | 1 | dx.doi.org/10.17504/protocols.io.81wgbx9eolpk/v1 | https://www.protocols.io/view/imaging-axonal-calcium-dynamics-in-ex-vivo-mouse-b-c5jzy4p6 | Yan-Feng Zhang, Stephanie J Cragg | TITLE: Imaging axonal calcium dynamics in ex vivo mouse brain slices
AUTHORS: Yan-Feng Zhang, Stephanie J Cragg
[DESCRIPTION]
This protocol describes how to image calcium dynamics in striatal dopaminergic axons in ex vivo mouse brain slices. We imaged calcium transients in response to single and trains (4 pulses, 100 ... | ["[Image Acquisition] Change the exposure time to reach a frame rate of around 16.6 Hz every 2.5 min using Micro-Manager v1.4. 16.6 Hz frame rate every 2.5 min using Micro-Manager 1.4.", "[Image Analysis] The following steps were performed in MATLAB vR2019b and Fiji v1.5.\n\nExtract fluorescence intensity from the regio... |
51,742 | Isolation of Nucleated Cells from Whole Blood | 1 | dx.doi.org/10.17504/protocols.io.bwr6pd9e | https://www.protocols.io/view/isolation-of-nucleated-cells-from-whole-blood-bwr6pd9e | Steven B. Wells, Peter A. Szabo, Nora Lam | TITLE: Isolation of Nucleated Cells from Whole Blood
AUTHORS: Steven B. Wells, Peter A. Szabo, Nora Lam
[DESCRIPTION]
This protocol describes a method for the isolation of pan-lymphocytes and pan-myeloid cells from human whole blood. By providing defined media formulations, volumes at each step, and a defined dilution... | ["[Preparing Mediums and Buffers] Create the following IMDM-FBS-PSQ Media in a 500mL bottle of IMDM by using the table below: \n Component Volume (mL) Starting Conc. Final Conc.* IMDM 500 - - Penicillin-Streptomycin-Glutamine 5 100X 1X FBS 50 100% 10%", "[Preparing Mediums and Buffers] Create... |
102,454 | Crystallisation of SARS-CoV-2 Mpro | 1 | dx.doi.org/10.17504/protocols.io.261ge5emyg47/v1 | https://www.protocols.io/view/crystallisation-of-sars-cov-2-mpro-dgaw3sfe | blake.h.balcomb, Peter Marples, Lizbé Koekemoer, Daren Fearon, Charlie Tomlinson | TITLE: Crystallisation of SARS-CoV-2 Mpro
AUTHORS: blake.h.balcomb, Peter Marples, Lizbé Koekemoer, Daren Fearon, Charlie Tomlinson
[DESCRIPTION]
The COVID-19 pandemic has highlighted the need to identify novel therapeutic interventions and strategies for pandemic preparedness against Severe Acute Respiratory Syndrome... | ["[Crystallisation experiment] Protein and buffer requirements:\n43.2 µL5 mg/mL \n1.92 mL \n14.4 µL seeds, dilution 1:250", "[Crystallisation experiment] Dispense 20 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.\nDispense 150 nL5 mg/mL to each lens using the SPT mosquito.\nDis... |
28,855 | Dechorionation of zebrafish embryos with Pronase for metronidazole-mediated ß-cell ablation | null | dx.doi.org/10.17504/protocols.io.8exhtfn | null | Sandra Rieger | TITLE: Dechorionation of zebrafish embryos with Pronase for metronidazole-mediated ß-cell ablation
AUTHORS: Sandra Rieger
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block"><span>Zebrafish embryos develop in their eggshel... | ["[Potential Pitfalls:]\n1. The embryos do not survive after treatment: This could be if embryos were incubated too long in Pronase. Decrease the incubation time and increase the number of washes.2. Dechorionation does not work: Pronase might be too old or animals are older than 24 hours post fertilization. Increase in... |
33,918 | Untitled | 1 | dx.doi.org/10.17504/protocols.io.bdc6i2ze | https://www.protocols.io/view/untitled-bdc6i2ze | Cui Jian | TITLE: Untitled
AUTHORS: Cui Jian
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">l;l;l;</div></div>
[STEPS]
?. [1]
0 µl
0 µl
0 µl
?. [1]
{"blocks":[{"key":"8v8id","text":"The growing of microbrewerys in Brazil has imposing an increase in the production of different supplies for brewing, iincluding... | ["[1]\n0 µl\n0 µl\n0 µl", "[1]\n{\"blocks\":[{\"key\":\"8v8id\",\"text\":\"The growing of microbrewerys in Brazil has imposing an increase in the production of different supplies for brewing, iincluding yeast biomass for beer fermentation. In Brazil,only four companies produce and sell yeasts for microbreweries, and on... |
60,070 | Mouse lung MAIT cell expansion and purification protocol | 4 | dx.doi.org/10.17504/protocols.io.x54v9yrpqg3e/v1 | https://www.protocols.io/view/mouse-lung-mait-cell-expansion-and-purification-pr-b6werfbe | Gabriel A Ascui, Eleni Phung, Alba Mendis, Mitchell Kronenberg | TITLE: Mouse lung MAIT cell expansion and purification protocol
AUTHORS: Gabriel A Ascui, Eleni Phung, Alba Mendis, Mitchell Kronenberg
[DESCRIPTION]
Mucosal Associated Invariant T (MAIT) cells are unconventional T cells present abundantly in human tissues. MAIT cells interact with antigens presented by MR1, an MHC cl... | ["[MAIT cell in vivo expansion with BRD509] Salmonella enterica serotype Typhimurium BRD509 vaccine strain culture", "[MAIT cell in vivo expansion with BRD509] Day 0: Overnight culture of Salmonella Typhimurium BRD509 strain.\n\nPrepare 5 mL of LB media with 100 µg/ml of Streptomycin. \nPick -80 °C BRD509 stock and ino... |
79,519 | Prime Editing in Physcomitrium patens | 4 | dx.doi.org/10.17504/protocols.io.4r3l27r5qg1y/v1 | https://www.protocols.click/view/prime-editing-in-physcomitrium-patens-crv7v69n | Pierre-François Perroud, Anouchka Guyon-Debast , Josep M Casacuberta, Wyatt Paul, Jean-Philippe Pichon, David Comeau, Fabien Nogué | TITLE: Prime Editing in Physcomitrium patens
AUTHORS: Pierre-François Perroud, Anouchka Guyon-Debast , Josep M Casacuberta, Wyatt Paul, Jean-Philippe Pichon, David Comeau, Fabien Nogué
[DESCRIPTION]
PEG-mediated naked DNA protoplast transfection has been the standard transformation approach for more than thirty years... | ["Using a binocular lens, check carefully the starting P. patens material for an eventual contamination (bacteria, fungus). The tissue must be green. An axenic culture is essential before performing any further experimental step.", "Let the protoplasts grow for 6 to 7 days on the transformation plates. Observe the plat... |
68,928 | Pole Test | 1 | dx.doi.org/10.17504/protocols.io.5jyl8938rv2w/v1 | https://www.protocols.io/view/pole-test-cfi8tkhw | Sabina Marciano, Tae-In Kam, Roberta Marongiu, Ted Dawson | TITLE: Pole Test
AUTHORS: Sabina Marciano, Tae-In Kam, Roberta Marongiu, Ted Dawson
[DESCRIPTION]
This behavior test is used to assess motor coordination in mice.
[GUIDELINES]
Main outcome measures: average time to turn, average time to reach the bottom
[STEPS]
1. Place the mouse on the vertical pole with their head... | ["Place the mouse on the vertical pole with their head oriented upwards 7.5cm from the top of the pole.", "During day 1, gently guide the mouse to turn around and go to the bottom of the pole. They are trained 3 times.", "On day 2, they are trained 3 times again without guidance.", "On day 3, the test day, mice are aga... |
18,732 | GUIDE-seq simplified library preparation protocol (CRISPR/Cas9 off-target cleavage detection) | null | dx.doi.org/10.17504/protocols.io.wikfccw | null | Nagendra palani | TITLE: GUIDE-seq simplified library preparation protocol (CRISPR/Cas9 off-target cleavage detection)
AUTHORS: Nagendra palani
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">GUIDE-seq is an experimental method to detect off-target cleavages caused during CRISPR/Cas editing.</div><div class = "text-b... | ["[Adaptor formation]\nUse 0.1x TE to prepare 100 μM stock of all the oligos.In a 0.2 ml PCR tube, add the following reagents, vortex well, and spin down. Place on the thermal cycler at for (heated lid). -------------------------------------------- After incubation, terminate the incubation and let the heat block... |
61,943 | Bioconjugation Strategies | 1 | null | https://www.protocols.io/view/bioconjugation-strategies-b8qxrvxn | BOC Sciences | TITLE: Bioconjugation Strategies
AUTHORS: BOC Sciences
[DESCRIPTION]
Bioconjugation is a chemical technique that is used to couple two molecules together, at least one of which is a biomolecule, such as carbohydrate, nucleic acid, or protein. Functionalization of polymers can provide improved stability, solubilit... | [] |
26,146 | What is CD14 | null | dx.doi.org/10.17504/protocols.io.5sag6ae | null | susan wind | TITLE: What is CD14
AUTHORS: susan wind
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">CD14, the LPS (Lipopolysaccharide) receptor, was originally a leukocyte differentiation antigen present on the surface of cells such as monocytes and macrophages. It was first discovered by TODD from the surface ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.q83dzyn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Fluorescent staining for Callose of sieve tubes of phloem.</strong></p>
<p>Calose (β-1→3-glucan) is the distinguished polysaccharide present on the sieve plates, of each sieve tube member of phloem tissue. Aniline blue stain specifically callose and it is use as a ma... | [] |
41,461 | SUPER rapid kit | 4 | dx.doi.org/10.17504/protocols.io.bkqvkvw6 | https://www.protocols.io/view/super-rapid-kit-bkqvkvw6 | Hyeon Jin Kim, Seong T. Hong | TITLE: SUPER rapid kit
AUTHORS: Hyeon Jin Kim, Seong T. Hong
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">We developed a supersensitive capillary reaction kit intended for the direct and qualitative detection of SARS-CoV-2 virus in saliva and nasal swabs. It enables sensitive and specific detecti... | ["Prepare the specimen by collecting saliva, sputum, or nasal swab for testing", "Dispense 2~5 drops of the specimen into the sampling tube (provided) filled with a general transfer pipette", "Mix the content by pipetting up & down gently", "Dispense 5 drops of mixed samples into the sample well of the cassette (provid... |
60,170 | Golden Gate Assembly | 4 | dx.doi.org/10.17504/protocols.io.x54v9yr3mg3e/v1 | https://www.protocols.io/view/golden-gate-assembly-b6zirf4e | Isaac Núñez, Tamara Matute, Fernan Federici | TITLE: Golden Gate Assembly
AUTHORS: Isaac Núñez, Tamara Matute, Fernan Federici
[DESCRIPTION]
The Golden Gate technique allows the assembly of genetic sequences from libraries of standardized basic components, which are cleaved from their donor vectors and concatenated in the acceptor vector in a defined order. Th... | ["[Preparation of the DNA components] Prepare plasmids stock solutions of the desired components to be used.", "[Preparation of the DNA components] Measure the concentration of purified plasmids.", "[Preparation of the DNA components] Perform dilutions of the plasmids to working concentrations. \nIt is:\n15 fmol/μL for... |
82,281 | Eastern Hemlock Tissue Collection for DNA | 4 | null | https://www.protocols.click/view/eastern-hemlock-tissue-collection-for-dna-cukhwut6 | Karl Fetter | TITLE: Eastern Hemlock Tissue Collection for DNA
AUTHORS: Karl Fetter
[DESCRIPTION]
Steps for collecting tissue from Eastern Hemlocks for DNA analysis. The Plant Computational Genomics lab is conducting a landscape genomics study of climate adaptation in the Eastern Hemlock. This protocol is a step-by-step guide to co... | ["[Introduction] This protocol is intended for field collection of leaf tissue for the Eastern Hemlock conservation genomics project sponsored by the Plant Computation Genomics lab at the University of Connecticut. The goal of the project is to identify climate adapted genomic variation for seed banking and potential u... |
96,260 | REDI-NET F-1 FECES FIELD SAMPLING | 0 | dx.doi.org/10.17504/protocols.io.yxmvm3dx6l3p/v1 | https://www.protocols.io/view/redi-net-f-1-feces-field-sampling-c99cz92w | REDI-NET Consortium | TITLE: REDI-NET F-1 FECES FIELD SAMPLING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
To outline steps for properly collecting fecal samples from pastures to evaluate the risk of zoonotic disease transmission by the detection of pathogens from vertebrate DNA (vDNA).
[BEFORE_START]
Know the risks associated with the stu... | ["[SAMPLE SITE SELECTION] Locate a fecal sample and estimate its age in days and score its consistency.", "[SAMPLE SITE SELECTION] Age Estimate", "[SAMPLE SITE SELECTION] Consistency Score - Cattle (see photos in Appendix 1)", "[SAMPLE SITE SELECTION] Consistency Score - Sheep (see photos in Appendix 2)", "[SAMPLE SITE... |
60,297 | Nuclei isolation from frozen human brain samples for snRNA-seq | 4 | dx.doi.org/10.17504/protocols.io.j8nlkk9ydl5r/v1 | https://www.protocols.io/view/nuclei-isolation-from-frozen-human-brain-samples-f-b65hrg36 | Marcos Nascimento | TITLE: Nuclei isolation from frozen human brain samples for snRNA-seq
AUTHORS: Marcos Nascimento
[DESCRIPTION]
Protocol to isolate nuclei from snap-frozen human brain samples for sn-RNAseq
[STEPS]
SECTION: Preparation
2. Clean your work area (bench and pipettes) with RNAse Zap.
SECTION: Nuclei Isolation
6. Transfer t... | ["[Preparation] Clean your work area (bench and pipettes) with RNAse Zap.", "[Nuclei Isolation] Transfer the LB-sample mix to a labelled glass dounce homogenizer . Add 2ml of LB, bringing the total volume in the douncer to 3mL.on ice \nYou can add 1ml of LB to the sample eppendorf to collect any remaining tissue.", "[N... |
null | null | null | dx.doi.org/10.17504/protocols.io.hd5b286 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
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98,447 | CODA: shorthand for calling functions | HuBMAP | JHU-TMC | 0 | dx.doi.org/10.17504/protocols.io.5qpvoknkbl4o/v2 | https://www.protocols.io/view/coda-shorthand-for-calling-functions-hubmap-jhu-tm-dcdp2s5n | Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz | TITLE: CODA: shorthand for calling functions | HuBMAP | JHU-TMC
AUTHORS: Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz
[DESCRIPTION]
To downsample ndpi or svs images to 10x, 5x, and 1x tifs, use this function:
create_downsampled_tif_images
or try Openslide in pytho... | ["[shorthand in the abstract] Use the above shorthand to facilitate your workflow by using it as a \"cheat sheet\""] |
null | null | null | dx.doi.org/10.17504/protocols.io.c9iz4d | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol combines phage gene detection with rRNA detection for the identification of host cells and detection of free phage particles.
[BEFORE_START]
<strong>Before staring the phageFISH experiment: Probe Design, Probe Synthesis, and Hybridization Stringency:</strong><br /... | [] |
25,576 | Expression and purification of (GST-tagged) (Kai) proteins | null | dx.doi.org/10.17504/protocols.io.48ggztw | null | Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann | TITLE: Expression and purification of (GST-tagged) (Kai) proteins
AUTHORS: Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka Maria Axmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol can be used for:</div><div class ... | ["[heterologous protein expression in E.coli]\ntransformation:", "[heterologous protein expression in E.coli]\npre-culture:inoculate 100 ml terrific broth medium containing 100 µg ampicillin ml-1 with resulting transformantsincubate over night at 37 °C and 200-250 r.p.m.", "[heterologous protein expression in E.coli]\n... |
23,774 | RNA extractions from de-etiolated Arabidopsis seedlings using CTAB | null | dx.doi.org/10.17504/protocols.io.3f6gjre | null | Akila Wijerathna-Yapa, Andrew Bowerman, Diep Ganguly | TITLE: RNA extractions from de-etiolated Arabidopsis seedlings using CTAB
AUTHORS: Akila Wijerathna-Yapa, Andrew Bowerman, Diep Ganguly
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A CTAB based method for extracting RNA, which is particularly useful for tough tissues. This has been adapted from a... | ["Harvest tissues into liquid nitrogen and grind (under liquid nitrogen) using mortar and pestle to obtain a fine powder. Ground tissue should be kept in safe-lock tubes in liquid nitrogen (or returned to -80C storage) until all samples are processed. This step is critical for efficient extractions so take your time he... |
22,315 | Universal DNA isolation protocol | null | dx.doi.org/10.17504/protocols.io.z2jf8cn | null | Ruslan Kalendar | TITLE: Universal DNA isolation protocol
AUTHORS: Ruslan Kalendar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The isolation of nucleic acids from a sample is an important step for many molecular biological applications and medical diagnostic assays. This protocol describes an efficient method for... | ["In 2 ml tube with mechanically disrupted seeds/leaves/herbarium or DNA solution (CTAB purification) add 1 ml CTAB solution buffer with RNAse A (the sample mass should not exceed 100 mg), vortex very well and incubate the samples at 60-65°C during 60-120 min or longer (long incubation increases DNA yield).", "Centrifu... |
28,402 | MojoSort™ Mouse NK Cell Isolation Kit Protocol | null | dx.doi.org/10.17504/protocols.io.7yshpwe | null | Sam Li | TITLE: MojoSort™ Mouse NK Cell Isolation Kit Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Product description and procedure summary:</span><span> Target cells are depleted by incubating your sample with the biotin antibody cocktail follow... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) polypropylene tube.Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells with... |
27,559 | Human embryonic gonad dissociation with Trypsin-EDTA | 1 | dx.doi.org/10.17504/protocols.io.66fhhbn | https://www.protocols.io/view/human-embryonic-gonad-dissociation-with-trypsin-ed-66fhhbn | Regina Hoo, Roser Vento-Tormo, Carmen Sancho | TITLE: Human embryonic gonad dissociation with Trypsin-EDTA
AUTHORS: Regina Hoo, Roser Vento-Tormo, Carmen Sancho
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for enrichment of fetal gonadal cells</div></div>
[STEPS]
?. Samples arrive in hypothermosol solution. The gonad is wash... | ["Samples arrive in hypothermosol solution. The gonad is washed with PBS and dissociated into single-cells following the protocol below.", "Fragment the gonads with a scalpel into 2 to 20 pieces per gonad (depending on developmental stage).", "Suspend gonadal fragments into a 15 mL tube containing of Trypsin-EDTA (0.25... |
94,180 | Barnes Maze Protocol | 4 | dx.doi.org/10.17504/protocols.io.kxygx3bozg8j/v1 | https://www.protocols.io/view/barnes-maze-protocol-c78czrsw | Lisa Blackmer-Raynolds, Ian N Krout, Tim Sampson | TITLE: Barnes Maze Protocol
AUTHORS: Lisa Blackmer-Raynolds, Ian N Krout, Tim Sampson
[DESCRIPTION]
The Barnes Maze test is a test of rodent’s spatial learning and memory abilities. In this test, animals are placed in an aversive environment (with a loud noise and bright lights) and trained to locate a hidden escape b... | ["[Set-up] Set up extra bright overhead lamps directly above maze so there is no difference in the brightness across the maze.", "[Set-up] Set white noise maker to highest volume setting (butkeep off for now).", "[Set-up] Make sure there are landmarks around the room that the animals can use to orient, everything in th... |
27,910 | private parent | null | dx.doi.org/10.17504/protocols.io.7hehj3e | null | Monica Hassan | TITLE: private parent
AUTHORS: Monica Hassan
[STEPS]
?. music | ["music"] |
19,753 | Bioelectrochemistry protocol For CHI Potentiostat | null | dx.doi.org/10.17504/protocols.io.xihfkb6 | null | Rose Jones Jones, Beth Orcutt | TITLE: Bioelectrochemistry protocol For CHI Potentiostat
AUTHORS: Rose Jones Jones, Beth Orcutt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The following protocol describes how to prepare microbial fuel cells (MFCs) with Indium Tin Oxide (ITO) electrodes for use as a poised potential enrichment ... | ["Preparing ITO “working” electrodes and carbon cloth “counter” electrodes Measure and cut 5 lengths of Ti wire 8cm long. Rub well with 100% ethanol and kimwipe to remove impurities (These will show up as black marks on the kimwipe. Measure and cut four 1.5” x 1.5” pieces of carbon cloth for the counter electrodes, an... |
91,122 | F-5 FECES SHIPPING | 4 | dx.doi.org/10.17504/protocols.io.x54v9pzy4g3e/v1 | https://www.protocols.io/view/f-5-feces-shipping-c48syzwe | REDI-NET Consortium | TITLE: F-5 FECES SHIPPING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol describes feces shipping.
[GUIDELINES]
OBJECTIVE
To outline steps for proper packaging and shipping of preserved feces samples from a REDI-NET Silver Lab to a REDI-NET Gold Lab.
SUMMARY/SCOPE
The overarching aim of the REDI-NET is t... | ["[SAMPLE PREPARATION] Primary holding sample holding containers must be leak-proof. Samples should be preserved in appropriate preservative, if applicable (REDI-NET Feces Processing SOP F-2). Ensure that the lids are tightly closed to prevent leaking of storage media while in transit.", "[SAMPLE PREPARATION] Pack vial... |
36,002 | Cell line information | 1 | dx.doi.org/10.17504/protocols.io.rm7vz82o8vx1/v1 | https://www.protocols.io/view/cell-line-information-bfeajjae | Philippa R Kennedy | TITLE: Cell line information
AUTHORS: Philippa R Kennedy
[DESCRIPTION]
An amalgamation of cell line data in the lab. Its origin and standard culture conditions.
[STEPS]
SECTION: Lung
3. Non-small cell lung cancers:
Lung adenocarcinomas:
1. A549 (RRID:CVCL_0023) was purchased from the American Type Culture Collect... | ["[Lung] Non-small cell lung cancers: \n\nLung adenocarcinomas: \n1. A549 (RRID:CVCL_0023) was purchased from the American Type Culture Collection (ATCC) in March 2019. \n2. NCI-H322 (RRID:CVCL_1556; purchased May 2019, Sigma Aldrich)\n3. NCI-H522 (RRID: CVCL_1567).\n\nLarge cell lung carcinoma: \n1. NCI-H460 (RRID:CVC... |
null | null | null | dx.doi.org/10.17504/protocols.io.dp55q5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in "<a href="https://www.protocols.io/view/Isolation-of-cyanophages-by-liquid-bioassays-dp25qd" target="_blank">Isolation of cyanophages by liquid bioassays</a>"
[STEPS]
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.paudiew | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
95,842 | Elutriator operation protocol | 0 | dx.doi.org/10.17504/protocols.io.36wgq3x6olk5/v1 | https://www.protocols.io/view/elutriator-operation-protocol-c9uaz6se | Casey A Schlenker | TITLE: Elutriator operation protocol
AUTHORS: Casey A Schlenker
[DESCRIPTION]
Operation of The OSU Soybean Pathology and Nematology elutriator
[STEPS]
SECTION: Turning elutriator on
1. Turn on the water supply by turning the red valve on the wall counterclockwise. Open the flow for both hoses by turning the small yel... | ["[Turning elutriator on] Turn on the water supply by turning the red valve on the wall counterclockwise. Open the flow for both hoses by turning the small yellow handles parallel to the hoses.", "[Turning elutriator on] Turn on the air compressor, located outside the greenhouse door behind the metal gate", "[Turning e... |
54,254 | Mercury Sequence and Sample Metadata Prep for Submission Workflow on the Terra Platform | 5 | null | https://www.protocols.io/view/mercury-sequence-and-sample-metadata-prep-for-subm-by8npzve | Jill V Hagey, Kevin Libuit, Lingzi Xiaoli, Technical Outreach and Assistance for States Team | TITLE: Mercury Sequence and Sample Metadata Prep for Submission Workflow on the Terra Platform
AUTHORS: Jill V Hagey, Kevin Libuit, Lingzi Xiaoli, Technical Outreach and Assistance for States Team
[DESCRIPTION]
Public health laboratories are encouraged to submit sequencing data for SARS-CoV-2 to multiple public dat... | ["[Where to Begin] This workflow is intended for users of Titan workflows for SARS-CoV-2 strain characterization on the Terra platform and expects the following protocols have already been completed: \n\nTerra and Google Cloud Accounts\nSamples uploaded\nOutput from the Titan workflow\n\nThis protocol is intended only ... |
58,874 | Beam Break and Nesting | 4 | dx.doi.org/10.17504/protocols.io.b5q2q5ye | https://www.protocols.io/view/beam-break-and-nesting-b5q2q5ye | Haley Geertsma | TITLE: Beam Break and Nesting
AUTHORS: Haley Geertsma
[DESCRIPTION]
This protocol is used to test mice in the Beam Break and Nesting behavioural test.
[STEPS]
2. Single-cage mice in the Beam Break rack with enough food and water for 48-hours.
1. Start Fusion software to begin recording and confirm the lights are on... | ["Single-cage mice in the Beam Break rack with enough food and water for 48-hours.", "Start Fusion software to begin recording and confirm the lights are on the appropriate light-dark cycle.", "Turn off Fusion software and add a square of cotton nestlet. Leave mice for 17-19 hours.", "Record and/or score nests as per h... |
71,672 | Growing Arabidopsis on nutrient-rich media | 4 | dx.doi.org/10.17504/protocols.io.bp2l6917dlqe/v1 | https://www.protocols.io/view/growing-arabidopsis-on-nutrient-rich-media-ch8yt9xw | Diep R Ganguly | TITLE: Growing Arabidopsis on nutrient-rich media
AUTHORS: Diep R Ganguly
[DESCRIPTION]
Notes on growing Arabidopsis seedlings on nutrient-rich media in plates.
[STEPS]
SECTION: Prepare media
1. Select and prepare autoclaved media of choice (see recipes below). Make sure to pH before adding gelling agent.
MS basa... | ["[Prepare media] Select and prepare autoclaved media of choice (see recipes below). Make sure to pH before adding gelling agent. \n\n MS basal salts\t 2.15 – 4.33 g/LSucrose10-30 g/LVitamins (1000X) (optional)1 mL/L\tMES (optional)0.1% (w/v)KOHpH 5.75Agar/Phytoblend8 g/L\n\n Gamborg’s B-5 basal medium3.21 g/LSucrose... |
26,842 | New Iron Extracting Method from Cattle's Blood for Iron Concentration Analysis | null | dx.doi.org/10.17504/protocols.io.6f2hbqe | null | Khairil Asnan, Mohd. Sadzan Asram bin Aliming, Aprialdy Idrus, Diana Eka Pratiwi | TITLE: New Iron Extracting Method from Cattle's Blood for Iron Concentration Analysis
AUTHORS: Khairil Asnan, Mohd. Sadzan Asram bin Aliming, Aprialdy Idrus, Diana Eka Pratiwi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"><span>Cattle's blood com... | ["[Sample preparation]\nCompress the whole blood sample from cattle.\n6 ml\n37 °C", "[Sample preparation]\nMix sample with\n[blood sample]\n[NaOH]\n37 °C\ndark green solution with strong odor", "Chelate reaction by adding into the previous mixed sample solution then let it sit for . After that, mixed them by for .\... |
21,888 | RNAi by feeding in Euplotes focardii (povisional) | null | dx.doi.org/10.17504/protocols.io.zk8f4zw | null | Francesca Papi, Angela Piersanti | TITLE: RNAi by feeding in Euplotes focardii (povisional)
AUTHORS: Francesca Papi, Angela Piersanti
[STEPS]
?. Grow RNAse III deficient E.coli strain HT115 in LB with antibiotic selection o/n at 37°C.
?. Prepare a 1:100 dilution of the bacterial culture, and grow it at 37°C until it reaches an OD600 of 0.4.
?. Add 0.4 ... | ["Grow RNAse III deficient E.coli strain HT115 in LB with antibiotic selection o/n at 37°C.", "Prepare a 1:100 dilution of the bacterial culture, and grow it at 37°C until it reaches an OD600 of 0.4.", "Add 0.4 mM IPTG, and induce RNA transcription from the L4440 plasmid, in which it is included the gene of interest t... |
33,612 | Case Processing SOP (Spleen) | null | dx.doi.org/10.17504/protocols.io.bc3kiykw | null | Marda Jorgensen | TITLE: Case Processing SOP (Spleen)
AUTHORS: Marda Jorgensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The purpose of this Standard Operating Procedure is to outline procedures for processing and storing spleen received for HuBMAP consortium assay and analysis. </div></div>
[STEPS]
?. The tis... | ["The tissues received will be identified as follows: Spleen (SP)", "Sample prepared from tissues will be identified using the following nomenclature, abbreviations, and formats: Formalin Fixed Paraffin Embedded---FFPE (paraffin block)Formalin Fixed Frozen Embedded---Fixed OCT BlockFresh Frozen Embedded---Fresh OCT Blo... |
59,440 | test protocol | 4 | null | https://www.protocols.io/view/test-protocol-b6aqradw | Julien J Ghislain | TITLE: test protocol
AUTHORS: Julien J Ghislain
[DESCRIPTION]
xx
[STEPS]
1. xxx
2. xxx | ["xxx", "xxx"] |
40,962 | VISUALIZING PROTEINS ON PAGE GEL WITH COOMASSIE COLLOIDAL STAIN - CHEM 584 | 4 | dx.doi.org/10.17504/protocols.io.bj9akr2e | https://www.protocols.io/view/visualizing-proteins-on-page-gel-with-coomassie-co-bj9akr2e | Ken Christensen | TITLE: VISUALIZING PROTEINS ON PAGE GEL WITH COOMASSIE COLLOIDAL STAIN - CHEM 584
AUTHORS: Ken Christensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Staining polyacrylamide gels using a colloidal Coomassie Blue stain. Adapted from a JoVE article.</div></div>
[STEPS]
?. Wash gel with ~50 mL dd... | ["Wash gel with ~50 mL ddH2O 3x for ~ for each wash on a shaker NOTE: insufficient washing causes poor sensitivity because the remaining SDS on the gels disturbs the bounding of the dye to the protein", "Shake the colloidal Coomassie solution before use to disperse particles evenly.", "Incubate the gels with the Coomas... |
90,064 | Sectioning and HE staining of Mouse Brain and embryo by Cryostat | 4 | dx.doi.org/10.17504/protocols.io.5qpvo379dv4o/v1 | https://www.protocols.io/view/sectioning-and-he-staining-of-mouse-brain-and-embr-c37qyrmw | Yuting Fu, jiashikai, Xiaodong Liu | TITLE: Sectioning and HE staining of Mouse Brain and embryo by Cryostat
AUTHORS: Yuting Fu, jiashikai, Xiaodong Liu
[DESCRIPTION]
This protocol describes how to use the cryostat to prepare and slice mouse brain and embryo sections for HE staining and following spatial transcriptomic experiments.
[STEPS]
SECTION: Prep... | ["[Preparation Methods for E12.5 mouse-embryo-eye] Mouse embryos were collected from pregnant C57BL/6J female mice at embryonic day 12.5\n(E12.5). Mouse brain was dissected from 8-week-old C57BL/6J male mice;", "[Preparation Methods for E12.5 mouse-embryo-eye] E12.5 pregnant female mice was anesthetized with carbon dio... |
71,313 | Extraction of Herbarium material for chemical profiling | 1 | null | https://www.protocols.io/view/extraction-of-herbarium-material-for-chemical-prof-chvrt656 | Ricardo M. Borges | TITLE: Extraction of Herbarium material for chemical profiling
AUTHORS: Ricardo M. Borges
[DESCRIPTION]
From the perspective of the documentation of plant biological diversity, herbaria are the repository of intraspecific and interspecific variability. The rich assortments contained in these collections essay the div... | ["[Sample preparation] Organize every sample that will be used in each study into an EXCEL Sheet;\ninclude information and comments on every sample\nhave information about classification grouping on every sample\neach sample should also have the filename for their analytical data indexed (these can be added afterward)\... |
42,390 | Mobile Device Quantification of Lateral Flow Tests: Modular illumination and sensor chamber (US patent US20160131592A1) | 3 | dx.doi.org/10.17504/protocols.io.bmmwk47e | https://www.protocols.io/view/mobile-device-quantification-of-lateral-flow-tests-bmmwk47e | Donald Cooper | TITLE: Mobile Device Quantification of Lateral Flow Tests: Modular illumination and sensor chamber (US patent US20160131592A1)
AUTHORS: Donald Cooper
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ea6bahe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is part of the VERVE holiday drive to collect exotic off-the-shelf laboratory recipes. More details <a href="https://www.protocols.io/g/verve-net/news/call-for-recipes" target="_blank">here</a>.
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?... | [] |
64,610 | Via Keto Gummies Reviews UK: Is It Best Fat Burner | 1 | dx.doi.org/10.17504/protocols.io.6qpvr6w6ovmk/v1 | https://www.protocols.io/view/via-keto-gummies-reviews-uk-is-it-best-fat-burner-cbcasise | viaketohack | TITLE: Via Keto Gummies Reviews UK: Is It Best Fat Burner
AUTHORS: viaketohack
[DESCRIPTION]
These days, many individuals are attempting to keep up with solid weight and every one of these are expected to help them in having a sound life. In any case, getting in shape while having solid absorption and common heartbe... | [] |
95,357 | Standardised Methods for Feed Tabs Preparation to Assess Feeding Rate and Exposure to Insoluble Substances in Parhyale hawaiensis | 4 | dx.doi.org/10.17504/protocols.io.81wgbx3qylpk/v1 | https://www.protocols.io/view/standardised-methods-for-feed-tabs-preparation-to-c9c5z2y6 | Ibrahim Lawan, Dominique MJ Anderson, Theodore, B. Henry | TITLE: Standardised Methods for Feed Tabs Preparation to Assess Feeding Rate and Exposure to Insoluble Substances in Parhyale hawaiensis
AUTHORS: Ibrahim Lawan, Dominique MJ Anderson, Theodore, B. Henry
[DESCRIPTION]
This protocol outlines standardised procedures for preparing feed tabs to assess feeding rates and con... | ["[Introduction] Dietary exposure and toxicological assessments of insoluble substances represent emerging frontiers in aquatic ecotoxicology, necessitating the development of standardised bioassays that accurately reflect real-world exposure scenarios (Connon et al., 2012). Current toxicity testing methodologies prima... |
null | null | null | dx.doi.org/10.17504/protocols.io.cpdvi5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Protocol for non-radioactive phosphorylation with T4 PNK.
[STEPS]
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.fd3bi8n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Instructions to make 50 mL of incubation buffer for protoplast isolation.</p>
<p>Adapted from Yoo et al. 2007 http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.html</p>
[STEPS]
?.
?.
?. | [] |
62,574 | Canadian Airborne Biodiversity Observatory's Forest Inventory Field Survey Protocol | 1 | dx.doi.org/10.17504/protocols.io.q26g7rn23vwz/v2 | https://www.protocols.io/view/canadian-airborne-biodiversity-observatory-39-s-fo-b9cnr2ve | Anna L Crofts, Sabine St-Jean, Mark Vellend | TITLE: Canadian Airborne Biodiversity Observatory's Forest Inventory Field Survey Protocol
AUTHORS: Anna L Crofts, Sabine St-Jean, Mark Vellend
[DESCRIPTION]
Here, we describe the standardized protocol used by the Canadian Airborne Biodiversity Observatory (CABO) to conduct the field-based surveys of canopy tree... | ["[Plot Establishment in the Field and in Fulcrum] Systematically establish plots to span the range of conditions across the imaged area. Specifically, we aim to:\n\n Maximize the number of unique tree species assemblages\n Cover the range of topographic conditions (e.g., elevation, slope, and aspect)", "[Obtai... |
98,735 | Patient PBMC collection and cryopreservation and cryorecovery | 0 | dx.doi.org/10.17504/protocols.io.n92ld895ov5b/v1 | https://www.protocols.io/view/patient-pbmc-collection-and-cryopreservation-and-c-dcnp2vdn | Rebecca Wallings | TITLE: Patient PBMC collection and cryopreservation and cryorecovery
AUTHORS: Rebecca Wallings
[DESCRIPTION]
Isolation of PBMC from human blood using CPT tubes, cryopreservation and cryorecovery of cells
[STEPS]
SECTION: PBMC collection and cryopreservation
1. Collect 8 mL of blood per BD Vacutainer CPT Cell Preparat... | ["[PBMC collection and cryopreservation] Collect 8 mL of blood per BD Vacutainer CPT Cell Preparation Tube with Sodium Citrate (BD Biosciences, 362761). \n\nInvert tubes 8-10 times and centrifuged at room temperature at 1500x g for 20 minutes with no brake. \n\nUsing a 10mL stripette , transfer the PBMC enriched layer ... |
58,462 | Magnitude of job satisfaction and intention to leave present job among nurses in selected Federal Hospitals in Addis Ababa, Ethiopiantitled collection | 2 | dx.doi.org/10.17504/protocols.io.b5b6q2re | https://www.protocols.io/view/magnitude-of-job-satisfaction-and-intention-to-lea-b5b6q2re | Aynye Negese*, Elsabet Getye2, ziyadahm | TITLE: Magnitude of job satisfaction and intention to leave present job among nurses in selected Federal Hospitals in Addis Ababa, Ethiopiantitled collection
AUTHORS: Aynye Negese*, Elsabet Getye2, ziyadahm
[DESCRIPTION]
Background
Job dissatisfaction issues and Health workers’ intention to leave is an increas... | [] |
90,468 | C-SOP-201: Genomic DNA Quantification using a Qubit Fluorometer | 4 | dx.doi.org/10.17504/protocols.io.kqdg3xb47g25/v1 | https://www.protocols.io/view/c-sop-201-genomic-dna-quantification-using-a-qubit-c4kcyusw | Mihir Kekre, Ben Pascoe | TITLE: C-SOP-201: Genomic DNA Quantification using a Qubit Fluorometer
AUTHORS: Mihir Kekre, Ben Pascoe
[DESCRIPTION]
In order to prepare high quality double-stranded DNA libraries for Illumina WGS, extracted genomic DNA needs to be accurately quantified. Qubit Fluorometers detect fluorescent dyes that are specific to... | ["[Before starting] The buffer and BR/HS reagent (dye) should be stored at 4 Room temperature while the standards must be stored at 4 °C. \n\nThe reagent is light and humidity sensitive, it should be stored in the dark and exposed minimally during assay and sample preparation. When stored as directed, the reagent has a... |
61,548 | cDNA Library Preparation for scRNA-seq (10x Genomics) | 5 | dx.doi.org/10.17504/protocols.io.bp2l6175rvqe/v1 | https://www.protocols.io/view/cdna-library-preparation-for-scrna-seq-10x-genomic-b8ckrsuw | molmer , Martin Lotz, Tony Mondala, Steven Head | TITLE: cDNA Library Preparation for scRNA-seq (10x Genomics)
AUTHORS: molmer , Martin Lotz, Tony Mondala, Steven Head
[DESCRIPTION]
Samples are processed using V2 barcoding chemistry kits of 10x Genomics. For each run, 10,000 cells from individual donors are labeled with distinct oligo-barcoded antibodies (cell h... | ["Single cells are isolated from human femoral articular cartilage using doi \ndx.doi.org/10.17504/protocols.io.14egn765qv5d/v1.", "Single cell suspensions are multiplexed and converted to barcoded scRNAseq libraries using the Chromium Single Cell 3’ Library, Gel Bead and Chip Kit (10x Genomics), and the BD™ Hu Single ... |
63,402 | Oprah Winfrey ACV Gummies Reviews: Shark tank gummy candy 2022 | 1 | dx.doi.org/10.17504/protocols.io.rm7vzy2nrlx1/v1 | https://www.protocols.io/view/oprah-winfrey-acv-gummies-reviews-shark-tank-gummy-b96ir9ce | oprahwinfreyreviewsads | TITLE: Oprah Winfrey ACV Gummies Reviews: Shark tank gummy candy 2022
AUTHORS: oprahwinfreyreviewsads
[DESCRIPTION]
Oprah Winfrey ACV Gummies Reviews: Shark tank gummy candy 2022
[STEPS]
1. Oprah Winfrey ACV Gummies Reviews: Shark tank gummy candy 2022
Oprah Winfrey Keto – The Supplement for a Spare Shape and Sli... | ["Oprah Winfrey ACV Gummies Reviews: Shark tank gummy candy 2022\n\nOprah Winfrey Keto – The Supplement for a Spare Shape and Slim Body! \n\nThere's a superb product out then in the request that we all know by the name that goes as the Oprah Winfrey Keto. This blog is a complete in- depth analysis of this outstanding a... |
93,935 | Protocol: Purification of DNA from Whole Blood using the QIAamp Blood Midi Kit (Spin Protocol) + QC with Qubit fluorometer | 6 | dx.doi.org/10.17504/protocols.io.n92ldmx3ol5b/v1 | https://www.protocols.io/view/protocol-purification-of-dna-from-whole-blood-usin-c7ypzpvn | Paula Saffie, Breeana Baker, Kimberley J Billingsley | TITLE: Protocol: Purification of DNA from Whole Blood using the QIAamp Blood Midi Kit (Spin Protocol) + QC with Qubit fluorometer
AUTHORS: Paula Saffie, Breeana Baker, Kimberley J Billingsley
[DESCRIPTION]
The QIAamp DNA Blood Midi procedure yield pure DNA ready for direct restriction digestion or amplification. With ... | ["[DNA extraction procedure]", "[Sample QC procedure (Qubit Fluorometer)] STANDARDIZE THE QUBIT (every time you use it or once a week according to lab protocol)", "[Sample QC procedure (Qubit Fluorometer)] Get0.2 mL Qubit tubes.", "[Sample QC procedure (Qubit Fluorometer)] In one strip, add10 mL of BR Standard 1.", "[S... |
97,794 | Visual frailty in the ageing population: A scoping review | 0 | dx.doi.org/10.17504/protocols.io.x54v92844l3e/v1 | https://www.protocols.io/view/visual-frailty-in-the-ageing-population-a-scoping-dbra2m2e | Godfrey Wanok, Emilie McSwiggan, Vasilis Raptis, Baljean Dhillon, Ian Underwood, Peter Cackett | TITLE: Visual frailty in the ageing population: A scoping review
AUTHORS: Godfrey Wanok, Emilie McSwiggan, Vasilis Raptis, Baljean Dhillon, Ian Underwood, Peter Cackett
[DESCRIPTION]
Visual frailty is a condition which has not been comprehensively explored by researchers and other professionals. This scoping review wi... | ["[Review questions] What is the definition of visual frailty?\nWhat domains can be used in the assessment of the condition?\nAre there assessment tools for the condition in the ageing\npopulation?\nWhat factors are associated with visual frailty in the ageing population?\nWhat is the relationship between frailty and A... |
50,160 | Acupuncture for glucose and lipid metabolic disorders of polycystic ovarian syndrome: A systematic review protocol | 1 | dx.doi.org/10.17504/protocols.io.bu8qnzvw | https://www.protocols.io/view/acupuncture-for-glucose-and-lipid-metabolic-disord-bu8qnzvw | Yang Wu, Tao Peng, Yu Chen, Li Huang, Bisong He, Shaobin Wei | TITLE: Acupuncture for glucose and lipid metabolic disorders of polycystic ovarian syndrome: A systematic review protocol
AUTHORS: Yang Wu, Tao Peng, Yu Chen, Li Huang, Bisong He, Shaobin Wei
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"><a href=... | ["Study registration and designThesystematic review protocol has been registered prospectively in the International Prospective Register of Systematic Reviews (PROSPERO) (CRD42020177846). This protocol is reported in line with the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) st... |
24,740 | Luminex Bead Conjugation | null | dx.doi.org/10.17504/protocols.io.4ecgtaw | null | Andrew Crowley | TITLE: Luminex Bead Conjugation
AUTHORS: Andrew Crowley
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for amine-coupling of proteins to magnetic Luminex beads</div><div class = "text-block"><span>Quantity as written: 1 x 10</span><span style = "vertical-align:super;">6</span><span> beads ... | ["[Activation]\nResuspend beads by briefly vortexing; dispense\n[(contains approx. 1.25 million beads)]", "[Activation]\nBEAD SEPARATION BLOCK", "[Activation]\nCollect volume by pulse centrifugation", "[Activation]\nPlace on magnetic tube rack, shielded from light\nProtocol tipPartially open the microcentrifuge tube be... |
47,359 | Analysis of genetic relatedness and paternity assignment in wild Guinea baboons (Papio papio) based on microsatellites | 1 | dx.doi.org/10.17504/protocols.io.bsg7nbzn | https://www.protocols.io/view/analysis-of-genetic-relatedness-and-paternity-assi-bsg7nbzn | Federica Dal Pesco, Franziska Trede, Dietmar Zinner, Fischer Julia | TITLE: Analysis of genetic relatedness and paternity assignment in wild Guinea baboons (Papio papio) based on microsatellites
AUTHORS: Federica Dal Pesco, Franziska Trede, Dietmar Zinner, Fischer Julia
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify... | ["Sample collection and storage:\n\t\t\t\t\t\t\t .justify:after {\n\t\t\t\t\t\t\t content: \"\";\n\t\t\t\t\t\t\t display:inline-block;\n\t\t\t\t\t\t\t width: 100%;\n\t\t\t\t\t\t\t }\n\t\t\t\t\t\t\tWe collected samples for genetic analysis non-invasively conducting fecal sampling of all identified individuals... |
86,461 | Challenging Beam Test | 1 | dx.doi.org/10.17504/protocols.io.dm6gp33r5vzp/v1 | https://www.protocols.io/view/challenging-beam-test-cyn5xvg6 | Sabina Marciano, Roberta Marongiu | TITLE: Challenging Beam Test
AUTHORS: Sabina Marciano, Roberta Marongiu
[DESCRIPTION]
The challenging beam test is used to measure motor performance, coordination, and balance
[STEPS]
SECTION: Setup
1. Lay a bench pad on the table and place the beam on top of the two empty cages ~3-4 cm of overhang.
SECTION: Setup
2... | ["[Setup] Lay a bench pad on the table and place the beam on top of the two empty cages ~3-4 cm of overhang.", "[Setup] Place the test subject’s home cage on the narrow end sideways so that the bream rests on top of the side wall.", "[Setup] Clean the beam with 70% ethanol and dry thoroughly before starting and after c... |
25,703 | 08 Exploration of expression condition | null | dx.doi.org/10.17504/protocols.io.5cfg2tn | null | TJUSLS China | TITLE: 08 Exploration of expression condition
AUTHORS: TJUSLS China
[STEPS]
?. Transform the plasmid into bacteria used to express target protein(e.g. E.coli BL21(DE3)).
?. Take monoclone in the culture plate into LB tube and cultivate in shaking incubator overnight(10-12h) to activate bacteria.
?. Test the OD600 numb... | ["Transform the plasmid into bacteria used to express target protein(e.g. E.coli BL21(DE3)).", "Take monoclone in the culture plate into LB tube and cultivate in shaking incubator overnight(10-12h) to activate bacteria.", "Test the OD600 number of bacteria, then pipe 5-10ul into each new 5 mL LB tube. Don’t forget to a... |
40,293 | How to prepare zebrafish brain tissue samples for biochemical assays | 6 | dx.doi.org/10.17504/protocols.io.bjkdkks6 | https://www.protocols.io/view/how-to-prepare-zebrafish-brain-tissue-samples-for-bjkdkks6 | Adrieli Sachett, Matheus Gallas-Lopes, Radharani , Greicy M M Conterato, Ana Herrmann, Angelo Piato | TITLE: How to prepare zebrafish brain tissue samples for biochemical assays
AUTHORS: Adrieli Sachett, Matheus Gallas-Lopes, Radharani , Greicy M M Conterato, Ana Herrmann, Angelo Piato
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Zebrafish are increasingly used as a model animal in neuroscience r... | ["[Preparations to collect animal tissue]\nBefore starting to collect animal tissue, it is important to prepare some settings in order to guarantee the appropriate preservation of the sample and, ultimately, the assessment of biochemical parameters;", "[Sample collection and processing]\nThe following steps should be c... |
100,932 | Senescent Cell Evaluations in Normal Tissues (SCENT) Mapping Center Bronchoscopy Protocol | 0 | null | https://www.protocols.io/view/senescent-cell-evaluations-in-normal-tissues-scent-detc3eiw | Dr. Patty j Lee, Dr. Monica Kraft, Dr. Andrew Nixon | TITLE: Senescent Cell Evaluations in Normal Tissues (SCENT) Mapping Center Bronchoscopy Protocol
AUTHORS: Dr. Patty j Lee, Dr. Monica Kraft, Dr. Andrew Nixon
[DESCRIPTION]
Cellular senescence is a stress-response, as well as a critical component of cell fate during development, repair, resilience, and normal aging. D... | ["[Goal and Objective] This project aims to collect, handle, store, and allocate normal healthy lung tissues and biofluids for constructing cellular senescence maps according to the standards established by the Steering Committee of the SenNet consortium. It seeks to identify senescent cell differences across the body,... |
24,699 | Agar Plate Preparation | null | dx.doi.org/10.17504/protocols.io.4c3gsyn | null | Andrew Crowley | TITLE: Agar Plate Preparation
AUTHORS: Andrew Crowley
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Default scale - 1 L (makes approx. 2.5 sleeves of plates)</div></div>
[STEPS]
?. Add powdered LB ( ) and agar () to deionized water ( )
25 g
15 g
1 L
n.b.Particulates may remain after addition, but... | ["Add powdered LB ( ) and agar () to deionized water ( )\n25 g\n15 g\n1 L\nn.b.Particulates may remain after addition, but these will dissolve during the autoclave cycle", "Autoclave using liquid cycle", "Allow media to cool to\n50 °C", "Select antibiotic from the list below:", "Add kanamycin and mix thoroughly\n50 mg... |
57,457 | MicroStone: Exploring the capabilities of the Artec Micro in scanning stone tools | 1 | dx.doi.org/10.17504/protocols.io.81wgb6781lpk/v1 | https://www.protocols.io/view/microstone-exploring-the-capabilities-of-the-artec-b4crqsv6 | Armando Falcucci | TITLE: MicroStone: Exploring the capabilities of the Artec Micro in scanning stone tools
AUTHORS: Armando Falcucci
[DESCRIPTION]
In this protocol, I describe how to scan small-sized lithics with the high-precision desktop 3D scanner Artec Micro. This scanner is particularly efficient when it comes to scan small objec... | ["[Part 1 - Object positioning] Use the Vise Jig provided by Artec to securely hold the artifact between its jaws (Fig. 1). The Vise Jig can be safely mounted thanks to the magnetic plate (i.e., the Gum Jig) on the Screw Jig. The object should be stable enough to avoid movements during the scanning process. I recommend... |
null | null | null | dx.doi.org/10.17504/protocols.io.exybfpw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
80,277 | RNA extraction from hairy roots of common bean (Phaseolus vulgaris L.) and cDNA synthesis | 4 | dx.doi.org/10.17504/protocols.io.8epv5jq24l1b/v1 | https://www.protocols.io/view/rna-extraction-from-hairy-roots-of-common-bean-pha-csmvwc66 | Ronal Pacheco Sánchez, Noreide Nava | TITLE: RNA extraction from hairy roots of common bean (Phaseolus vulgaris L.) and cDNA synthesis
AUTHORS: Ronal Pacheco Sánchez, Noreide Nava
[DESCRIPTION]
Extracting RNA for subsequent quantification of transcript levels by RT-qPCR requires high purity and concentration. When the amount of tissue is not abundant, as ... | ["[Extraction of total RNA] Macerate root tissue using liquid nitrogen.", "[Extraction of total RNA] Load 100 mg of macerated tissue into a 1.5 mL Eppendorf tube and add 1 mL of .", "[Extraction of total RNA] Mix by vortexing15 s and incubate for 5 min at room temperature.", "Centrifuge 11800 rpm, 15 min, 4 °C", "[... |
20,730 | Lipid Biomarker Extraction and Elution into different Fractions from sediment | null | dx.doi.org/10.17504/protocols.io.yg2ftye | null | Lucia Leierer, Antonio V. Herrera-Herrera, Margarita Jambrina-Enríquez | TITLE: Lipid Biomarker Extraction and Elution into different Fractions from sediment
AUTHORS: Lucia Leierer, Antonio V. Herrera-Herrera, Margarita Jambrina-Enríquez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol of a method used to obtain the total lipid extract (TLE) from archae... | ["[Preparation]\nOven drying of sediment samples at during and subsampling to of homogenized sediment\n5 g", "[Lipid Extraction]\nAdd dichloromethane/methanol (DCM/MeOH) 9:1 v/v to the sediment\n40 ml", "[Lipid Extraction]\nMix for in a sonicator (below )", "[Lipid Extraction]\nTransfer the solvent into a centrifuge t... |
102,123 | Environmental DNA Metabarcoding- Full Pipeline Collection | 0 | dx.doi.org/10.17504/protocols.io.eq2lywxwpvx9/v1 | https://www.protocols.io/view/environmental-dna-metabarcoding-full-pipeline-coll-dfyj3pun | Colleen Kellogg, Matt Lemay, rute.carvalho Carvalho, Andreas Novotny | TITLE: Environmental DNA Metabarcoding- Full Pipeline Collection
AUTHORS: Colleen Kellogg, Matt Lemay, rute.carvalho Carvalho, Andreas Novotny
[DESCRIPTION]
This is a collection of protocols used to analyze environmental DNA (eDNA) of seawater samples.
As part of the Hakai Institute Ocean Observing Program, biomolecul... | [] |
65,182 | TestoUltra Brief Information [REVIEWS]: SCAM & LEGIT | 1 | dx.doi.org/10.17504/protocols.io.6qpvr6ydbvmk/v1 | https://www.protocols.io/view/testoultra-brief-information-reviews-scam-amp-legi-cbv6sn9e | testolutraty | TITLE: TestoUltra Brief Information [REVIEWS]: SCAM & LEGIT
AUTHORS: testolutraty
[DESCRIPTION]
Getting more settled can cause a few troublesome issues with your sexual concurrence. That is the explanation we really want to edify you concerning another upgrade called TestoUltra Make Enhancement pills. One thing ... | [] |
62,682 | Small molecules released from islets of Langerhans determined by liquid chromatography – mass spectrometry | 4 | dx.doi.org/10.17504/protocols.io.6qpvr6rnzvmk/v1 | https://www.protocols.io/view/small-molecules-released-from-islets-of-langerhans-b9f2r3qe | Emmanuel O. Ogunkunle, Matthew J. Donohue, Daniel J. Steyer, Damilola I. Adeoye, Wesley J. Eaton, Michael Roper | TITLE: Small molecules released from islets of Langerhans determined by liquid chromatography – mass spectrometry
AUTHORS: Emmanuel O. Ogunkunle, Matthew J. Donohue, Daniel J. Steyer, Damilola I. Adeoye, Wesley J. Eaton, Michael Roper
[DESCRIPTION]
Islets of Langerhans are the endocrine tissue within the pancreas tha... | ["[Methods] Benzoyl chloride derivatization\n\nFor production of calibration curves, 100 µLof a standard mixture of small molecules (concentrations given in Section 3.3 in the original publication) in BSS was mixed with 50 µL of 100 mM sodium carbonate (pH 9.2) and 50 µL 2% BzCl (by volume in ACN). The mixture was vort... |
65,098 | Ikaria Lean Belly Juice Customer Reviews and Complaints! | 1 | dx.doi.org/10.17504/protocols.io.rm7vzyem5lx1/v1 | https://www.protocols.io/view/ikaria-lean-belly-juice-customer-reviews-and-compl-cbtisnke | komalyo | TITLE: Ikaria Lean Belly Juice Customer Reviews and Complaints!
AUTHORS: komalyo
[DESCRIPTION]
Everybody has the right to have a decent body shape and feel happy with just being themselves. It is in every case great to see your appearance in the mirror and be happy with your actual appearance. Sadly, few out of ... | [] |
64,680 | In situ high-speed brightfield imaging for studies of aquatic organisms | 4 | null | https://www.protocols.io/view/in-situ-high-speed-brightfield-imaging-for-studies-cbegsjbw | Sean P. Colin, Brad J. Gemmell, John H. Costello, Kelly R R Sutherland | TITLE: In situ high-speed brightfield imaging for studies of aquatic organisms
AUTHORS: Sean P. Colin, Brad J. Gemmell, John H. Costello, Kelly R R Sutherland
[DESCRIPTION]
Behavioral measurements of fragile aquatic organisms require specialized in situ techniques.We developed an in situ brightfield camera set-up ... | ["[Select field site] This system is lightweight, compact and portable and can be used SCUBA diving from shore, docks or boats.The camera system can also potentially be towed vertically from a research vessel.", "[Assemble equipment] The brightfield camera system relies entirely on available off the shelf components an... |
33,893 | Assembling Algal Shaker | null | dx.doi.org/10.17504/protocols.io.bdcdi2s6 | null | Jakub Nedbal | TITLE: Assembling Algal Shaker
AUTHORS: Jakub Nedbal
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol described the final assembly steps to complete the illuminated orbital shaker and its testing. The steps involve fixing the transparent platform to the orbital shaker, placing the LED i... | ["Prepare a tidy workbench with sufficient space and illumination to minimize risk of accidents.", "Fix the four M4×50 mm male-female standoffs to the orbital shaker platform using nuts and washers.Where Imperial threads are preferred, use #8-32×2\" stand-offs instead.", "Lay the cooled LED illuminator onto the orbita... |
62,438 | Lanthanum DAB metals, Ln-DAB2 labeling of APEX2 | 1 | dx.doi.org/10.17504/protocols.io.e6nvwkoe2vmk/v1 | https://www.protocols.io/view/lanthanum-dab-metals-ln-dab2-labeling-of-apex2-b88erzte | Stephen R. Adams, Mason R. Mackey, Alice Ting, Mark Ellisman, Thomas Deerinck | TITLE: Lanthanum DAB metals, Ln-DAB2 labeling of APEX2
AUTHORS: Stephen R. Adams, Mason R. Mackey, Alice Ting, Mark Ellisman, Thomas Deerinck
[DESCRIPTION]
This protocol uses APEX2, an engineered peroxidase that genetically targets a cellular region of interest, to oxidatively polymerize Ln-DAB2, (lanthanum chelates c... | ["HEK293T cells are cultured on imaging plates containing poly-d-lysine coated glass bottom No. 0 coverslips (P35GC-0-14C, MatTek Corporation).", "Cells are transiently transfected with either APEX2-H2B or mitochondrial matrix-APEX2 fusion using Lipofectamine 3000 (Life Technologies). APEX2 is fused to N-terminal of H2... |
72,464 | Electroporation Transformation Protocol in S. Cerevisiae | 4 | null | https://www.protocols.io/view/electroporation-transformation-protocol-in-s-cerev-cizquf5w | Stephanie Hood | TITLE: Electroporation Transformation Protocol in S. Cerevisiae
AUTHORS: Stephanie Hood
[DESCRIPTION]
Protocol adapted by Stephanie Hood, 09/2022, from:
An improved yeast transformation method for the generation of very large human antibody libraries
Overview:
This protocol calls for ~800-900 ul of cell pellet and... | ["[2-3 Days before starting experiment] Streak out yeast strains for electroporation protocol.\nDepending on how many cultures you are setting up you may need to streak more than 1 plate to get enough isolated colonies. Grow at 30°C for 2 days.", "[Day before experiment] Grow 5 ml culture(s) of S. cerevisiae cells over... |
61,452 | Slant board seed gemination and transplanting for hydroponic research | 4 | null | https://www.protocols.io/view/slant-board-seed-gemination-and-transplanting-for-b79krr4w | noah.langenfeld , Bruce Bugbee | TITLE: Slant board seed gemination and transplanting for hydroponic research
AUTHORS: noah.langenfeld , Bruce Bugbee
[DESCRIPTION]
Uniform seed germination is essential for hydroponic research. Conventional seed germination uses substrates such as peat moss or mineral wool. These substrates are difficult to remove f... | ["[Seeding] Obtain a clean acrylic slant board and lay flat on a hard, clean surface.", "[Seeding] Cut a piece of germination paper 10 cm in height and the same length as the slant board.", "[Seeding] Place germination paper on top of slant board.", "[Seeding] Place seeds on top of germination paper (about 9 cm from th... |
63,160 | Deposition of matrix using an M5 TM sprayer for high resolution MALDI analysis | 1 | null | https://www.protocols.io/view/deposition-of-matrix-using-an-m5-tm-sprayer-for-hi-b9wyr7fw | Angela Kruse, Martin Dufresne, Jamie Allen, Danielle Gutierrez, Jeff Spraggins | TITLE: Deposition of matrix using an M5 TM sprayer for high resolution MALDI analysis
AUTHORS: Angela Kruse, Martin Dufresne, Jamie Allen, Danielle Gutierrez, Jeff Spraggins
[DESCRIPTION]
This protocol describes the use of an HTX M5 TM sprayer to deposit small molecule matrices onto tissue sections for high reso... | ["[Autofluorescence Scan] Remove slides from freezer and place in desiccator for 30 min", "[Autofluorescence Scan] Collect autofluorescence scan using a Zeiss AxioScan Z1 (https://www.protocols.io/edit/autofluorescence-microscopy-data-acquisition-b39kqr4w)", "[TM Sprayer Setup] Set nitrogen flow to 8 psi. turn on TM sp... |
100,673 | Sucrose lysis buffer | 1 | dx.doi.org/10.17504/protocols.io.j8nlk8op1l5r/v1 | https://www.protocols.io/view/sucrose-lysis-buffer-dei93ch6 | Colleen Kellogg | TITLE: Sucrose lysis buffer
AUTHORS: Colleen Kellogg
[DESCRIPTION]
This protocol describes the preparation of sucrose lysis buffer to preserve DNA on sterivex filters. As part of the Hakai Institute Ocean Observing Program, biomolecular samples have been collected weekly, from 0 m to near bottom (260 m), to geneticall... | ["[Methods] Filter-sterilize and label bottle.", "[Methods] Top up water to 500 mL. (no need to pH this one!)", "[Methods] Add a stir bar and dissolve all the powder.", "[Methods] Add milliQ water to about the 400 mL line.", "[Methods] Add 25mL of 1M Tris to the beaker.", "[Methods] Add 40 mL of 0.5M EDTA to the beaker... |
25,511 | Dispensing agar into multiwell plates July 2019 updates | null | dx.doi.org/10.17504/protocols.io.46fgzbn | null | Ida Barlow | TITLE: Dispensing agar into multiwell plates July 2019 updates
AUTHORS: Ida Barlow
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Protocol for pouring agar into 96 well plates using Integra VIAFILL dispenser. Agar should be prepared in advance, and kept in 60</span><span style = "vertical-ali... | ["[Configure Integra VIAFILL]\nInsert large cassette (suitable for dispensing volumes from 5-9999μL) into the machine:", "[Configure Integra VIAFILL]\nConfigure X, Y, and Z settings for the multiwell plate by clicking on tool symbol -> stage alignment -> 96 well 8Ch.\nFor UNIPLATE96SQWLF 650U:X = 95.6Y = 4.2Z = -22.5",... |
49,485 | Systemic AAV vectors for widespread and targeted gene delivery in rodents | 3 | dx.doi.org/10.17504/protocols.io.bujmnuk6 | https://www.protocols.io/view/systemic-aav-vectors-for-widespread-and-targeted-g-bujmnuk6 | Rosemary C. Challis, Sripriya Ravindra Kumar, Ken Y. Chan, Collin Challis, Keith Beadle, Min J. Jang, Hyun Min Kim, Pradeep S. Rajendran, John D. Tompkins, Kalyanam Shivkumar, Benjamin E. Deverman, Viviana Gradinaru | TITLE: Systemic AAV vectors for widespread and targeted gene delivery in rodents
AUTHORS: Rosemary C. Challis, Sripriya Ravindra Kumar, Ken Y. Chan, Collin Challis, Keith Beadle, Min J. Jang, Hyun Min Kim, Pradeep S. Rajendran, John D. Tompkins, Kalyanam Shivkumar, Benjamin E. Deverman, Viviana Gradinaru
[DESCRIPTION]... | [] |
40,851 | Background and Rationale (Part 1 of Phase 3 study of Vaccine Candidate for COVID-19) | 1 | dx.doi.org/10.17504/protocols.io.bj5tkq6n | https://www.protocols.io/view/background-and-rationale-part-1-of-phase-3-study-o-bj5tkq6n | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | TITLE: Background and Rationale (Part 1 of Phase 3 study of Vaccine Candidate for COVID-19)
AUTHORS: Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is a collection of protocols for: "Phase 3 randomized, double-blinded, placebo-contro... | [] |
102,395 | Lysosomal Membrane Permeability (LMP) assay | 0 | dx.doi.org/10.17504/protocols.io.eq2lywxbevx9/v1 | https://www.protocols.io/view/lysosomal-membrane-permeability-lmp-assay-df833ryn | Scott Vermilyea | TITLE: Lysosomal Membrane Permeability (LMP) assay
AUTHORS: Scott Vermilyea
[DESCRIPTION]
This protocol details the requirements of the Lysosomal Membrane Permeability (LMP) assay.
[STEPS]
SECTION: Cell Culture/Treatments:
1. 0.5x10^4/ml of cells plated on coverslips.
SECTION: Imaging:
6. Leica Stellaries 8 confocal ... | ["[Cell Culture/Treatments:] 0.5x10^4/ml of cells plated on coverslips.", "[Imaging:] Leica Stellaries 8 confocal laser scanning microscope.", "[Cell Culture/Treatments:] PFF-488 treatment.", "[Cell Culture/Treatments:] PBS/trypsin (0.01%) wash.", "[Cell Culture/Treatments:] Fix cells using 4% PFA.", "[Cell Culture/Tre... |
null | null | null | dx.doi.org/10.17504/protocols.io.jskcncw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
48,412 | Shipping Embryos in a Portable Incubator | 4 | dx.doi.org/10.17504/protocols.io.bth4nj8w | https://www.protocols.io/view/shipping-embryos-in-a-portable-incubator-bth4nj8w | Eliab Estrada Cortés, Peter Hansen | TITLE: Shipping Embryos in a Portable Incubator
AUTHORS: Eliab Estrada Cortés, Peter Hansen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a protocol to describe how to ship non-frozen embryos</div></div>
[STEPS]
?. [ ]
Test the shipping incubator and make sure that it can remain powered f... | ["[ ]\nTest the shipping incubator and make sure that it can remain powered for at least 24 hours while operating with the battery. We currently use TO 42 from WTA https://www.wtavet.com.br/?lang=en but others work well also. The temperature is set to 38.5°C", "[ ]\nStart charging shipping incubator 1 day before shippi... |
67,586 | SMRT-Tag - Sensitive multimodal profiling of native DNA by transposase-mediated single-molecule sequencing | 4 | dx.doi.org/10.17504/protocols.io.e6nvwk3b9vmk/v1 | https://www.protocols.io/view/smrt-tag-sensitive-multimodal-profiling-of-native-cd9as92e | Scott Nanda, Ke Wu, Siva Kasinathan, vijay.ramani | TITLE: SMRT-Tag - Sensitive multimodal profiling of native DNA by transposase-mediated single-molecule sequencing
AUTHORS: Scott Nanda, Ke Wu, Siva Kasinathan, vijay.ramani
[DESCRIPTION]
Here we describe a protocol for SMRT-Tag: Single-Molecule Real-Time sequencing by Tagmentation - a transposase-mediated strategy f... | ["[gDNA QC] High Molecular Weight genomic DNA (HMW gDNA) is used as standard input for the SMRT-Tag method. \n\nTo reduce its viscosity, incubate at 37ºC for 5-10 min at low agitation speed (300 rpm). Pipette up and down 5–10 times using a p200 wide-bore pipette to ensure any clumps of DNA are dispersed.\n\n[OPTIONAL] ... |
null | null | null | dx.doi.org/10.17504/protocols.io.rwcd7aw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Protocol describing fluorescence immunohistochemistry for detection of thymosin beta 4 alongside CD31, smooth muscle actin and myosin heavy chain in PFA-fixed human cardiac tissue samples.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | ["Tissue was fixed overnight in 4% PFA and then dehydrated the following day with 2 hour washes of 70%, 80%, 90% and 100% ethanol. Tissue was then placed in 100% chloroform overnight. The following day the tissue was embedded in paraffin wax, allowed to set and then stored at 4ºC.", "Using a Leica rotary microtome, 10 ... |
72,184 | 3'-Biotinylation of mRNA | 1 | null | https://www.protocols.io/view/3-39-biotinylation-of-mrna-ciqyudxw | Clark Fritsch | TITLE: 3'-Biotinylation of mRNA
AUTHORS: Clark Fritsch
[DESCRIPTION]
This protocol is meant to efficiently biotinylate the 3'-end of an mRNA prepared for use in single-molecule FRET experiments. The biotinylated 3'-end of the mRNA is able to bind tightly to streptavidin or neutravidin for use in the stopped-flow c... | ["[Reagent Preparation] 1 mL 1M Na-acetate (pH=5.0):Add 52.5mg (MW 82.03) Na-acetate (64%) and 20.7 uL of 17.4 M Acetic acid (36%) in 800 uL of DEPC water, then make up the volume upto 1 ml by adding more DEPC water or you can use the purchased 3M Na-acetate (pH=5.2) and make it to 1M.", "[Reagent Preparation] 3M KCl:A... |
86,661 | Overcoming problematic growth phenotypes in organoids from patients with monogenic GI disease | 4 | dx.doi.org/10.17504/protocols.io.dm6gp33ypvzp/v1 | https://www.protocols.io/view/overcoming-problematic-growth-phenotypes-in-organo-cyvdxw26 | Katlynn Bugda Gwilt, Jay Thiagarajah | TITLE: Overcoming problematic growth phenotypes in organoids from patients with monogenic GI disease
AUTHORS: Katlynn Bugda Gwilt, Jay Thiagarajah
[DESCRIPTION]
Patient-derived organoids provide a unique model system to explore disease causing mutations ex vivo. By use of organoids from duodenal or colonic biopsies of... | ["[Thawing Frozen Lines] 300 x g Centrifuge at 300 x g for 5 minutes", "[Thawing Frozen Lines] Aspirate media leaving all of the residual matrigel from freezing behind", "[Thawing Frozen Lines] Add 200ul of fresh matrigel to the organoid/matrigel pellet", "[Thawing Frozen Lines] Gently resuspend using P1000 and 1000ul ... |
null | null | null | dx.doi.org/10.17504/protocols.io.e4abgse | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p><strong>INTRODUCTION </strong></p>
<p>Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers is to select a protein assay from the vast selection on the market that is compatible... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.p3tdqnn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Here we describe the standardised protocol used by the <a href="http://www.caboscience.org" target="_blank" rel="noopener noreferrer">Canadian Airborne Biodiversity Observatory</a> (CABO) to measure leaf water content and specific leaf area, using the <a href="http://regent.q... | [] |
45,063 | Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010) | 1 | dx.doi.org/10.17504/protocols.io.bp9fmr3n | https://www.protocols.io/view/quick-protocol-for-monarch-plasmid-miniprep-kit-ne-bp9fmr3n | New England Biolabs | TITLE: Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010)
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is the quick version of the Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010). For the full protocol, please click </span><a href="https:... | ["Pellet 1–5 ml (not to exceed 15 OD units) bacterial culture by centrifugation for 30 seconds. Discard supernatant.\n1.5 ml of culture is sufficient for most applications. Ensure cultures are not overgrown (12-16 hours is ideal).", "Resuspend pellet in 200 μl Plasmid Resuspension Buffer (B1).\nVortex or pipet to ensur... |
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