id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.mpdc5i6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?. | [] |
94,073 | Immunocytochemistry for the characterization of hiPSC to Motor Neuron differentiation | 4 | dx.doi.org/10.17504/protocols.io.6qpvr3zbzvmk/v3 | https://www.protocols.io/view/immunocytochemistry-for-the-characterization-of-hi-c74zzqx6 | Mallory Wright, ckremitz, William J Buchser | TITLE: Immunocytochemistry for the characterization of hiPSC to Motor Neuron differentiation
AUTHORS: Mallory Wright, ckremitz, William J Buchser
[DESCRIPTION]
This immunocytochemistry protocol is used for the characterization of IPSC differentiation into motor neurons using several biomarkers: neuroepithelial cells (... | ["Remove the medium from your cells", "FIX - Dilute 32% Paraformaldehyde solution to 4% PFA in 1X phosphate-buffered saline (PBS)", "Add 100uL of 4% PFA to each well in the 96-well plate. (1ml if using a 6-well plate). Incubate for 15 min at room temperature.", "Remove the fixative solution and wash with 1XPBS at 100ul... |
null | null | null | dx.doi.org/10.17504/protocols.io.ra2d2ge | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
35,349 | RNA extraction and precipitation for 47 mm PES and polycarbonate filters | null | dx.doi.org/10.17504/protocols.io.bervjd66 | null | Frank Ferrer-Gonzalez, Christa Smith | TITLE: RNA extraction and precipitation for 47 mm PES and polycarbonate filters
AUTHORS: Frank Ferrer-Gonzalez, Christa Smith
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is a modified version of Bryn Durham et al., 2015 for extracting RNA from filters. </div><div class = "text-bloc... | ["Prepare a 2.0 ml tube (low-binding) for each sample, with each tube containing 400 μl beads (mix of 200 μl 0.1 mm zirconium beads, 100 μl 0.5 mm glass beads, 100 μl 0.5 mm zirconium beads.\nGlass beads are only needed when processing phytoplankton. If extracting from bacteria only then replace glass beads with more 0... |
29,089 | ssDNA Extraktion | 1 | dx.doi.org/10.17504/protocols.io.8m9hu96 | https://www.protocols.io/view/ssdna-extraktion-8m9hu96 | Franziska Patzold | TITLE: ssDNA Extraktion
AUTHORS: Franziska Patzold
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">ssDNA Extraction Protocol</div><div class = "text-block"><span>This protocol is based on the Oligonucleotide extraction protocol of the NEB PCR & Clean-up Kit and the Qiagen DNeasy Protocol with a few ... | ["[Elution]\nPlace dry tissue in 1.5 ml microcentrifuge tube and grind it, using an oscillating mill (MM 400, Retsch) for 1 min at a frequency of 25 Hz, using two sterile stainless steel balls with a diameter of 3 mm", "[Lysis]\nInsert to the ground tissue mix well by handshaking, don´t vortex! and gently spin down the... |
40,864 | Ethical Considerations and Informed Consent (Part 11 of Phase 3 study of Vaccine Candidate for COVID-19) | 1 | dx.doi.org/10.17504/protocols.io.bj58kq9w | https://www.protocols.io/view/ethical-considerations-and-informed-consent-part-1-bj58kq9w | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | TITLE: Ethical Considerations and Informed Consent (Part 11 of Phase 3 study of Vaccine Candidate for COVID-19)
AUTHORS: Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is Part 11 of "Phase 3 randomized, double-blinded, placebo-contro... | [] |
86,868 | Dual staining for Ki67 protein and MKI67 transcript detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues | 4 | dx.doi.org/10.17504/protocols.io.kqdg3xxo7g25/v1 | https://www.protocols.io/view/dual-staining-for-ki67-protein-and-mki67-transcrip-cy3uxynw | Jayne E Wiarda, Crystal L Loving | TITLE: Dual staining for Ki67 protein and MKI67 transcript detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues
AUTHORS: Jayne E Wiarda, Crystal L Loving
[DESCRIPTION]
A protocol for staining of protein (Ki-67) and RNA (MKI67) in pig tissues
[BEFORE_START]
Starting specimens:
Starting samples = FFPE tiss... | ["[Baking] Before starting the assay: \nPreheat a dry oven to 60℃\nLoad slides for assay into vertical slide rack\n\nBaking\nBake slides 30 min 60℃\n----------Optional stopping point: store slides in a dry place & use within 1 week\n\nWhile slides bake:\nPrepare 0.05% PBS-T (can store at RT up to 1 month)\nPrepare 1X C... |
106,630 | Quantitative PCR for STMN2 expression in pi-N neurons | 0 | dx.doi.org/10.17504/protocols.io.261ge51oyg47/v1 | https://www.protocols.io/view/quantitative-pcr-for-stmn2-expression-in-pi-n-neur-dkde4s3e | Isabel Lam, Alain Ndayisaba, Vikram Khurana | TITLE: Quantitative PCR for STMN2 expression in pi-N neurons
AUTHORS: Isabel Lam, Alain Ndayisaba, Vikram Khurana
[DESCRIPTION]
Quantitative PCR for STMN2 expression in pi-N neurons
[STEPS] | [] |
45,464 | (Mick's) Trial K+ minimal Media Recipe, 1_16_2019 | 4 | null | https://www.protocols.io/view/mick-39-s-trial-k-minimal-media-recipe-1-16-2019-bqmymu7w | Elizabeth Fozo | TITLE: (Mick's) Trial K+ minimal Media Recipe, 1_16_2019
AUTHORS: Elizabeth Fozo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Trial K</span><span style = "vertical-align:super;">+ </span><span>minimal Media Recipes</span></div></div>
[STEPS]
?. [5X M9 Media without K]
33.9g/L Na2HPO45g... | ["[5X M9 Media without K]\n33.9g/L Na2HPO45g/L NH4Cl2.5g/L NaCl15g NaH2PO4 (was KH2PO4)", "[1L of Trial K+ Minimal Media]\n1. Add 200mL 5X M9 to 500mL MiliQ water.1mL of 1M MgSO4 - 1mM final0.1mL of 10mg/mL Thiamine - 1ug/mL5mL of 80% glycerol - 0.4% final5mL of 10mg/mL AA mix 1 - 50ug/mL final5mL of 10mg... |
null | null | null | dx.doi.org/10.17504/protocols.io.kcjcsun | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
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?.
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?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.k95cz86 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Here I have summarized overall scheme and strategies I have adapted to perform immunofluorescent or immunohistochemistry on paraffin embedded tissue. The pdf file contains all these protocols and my accompanying notes. </p>
[STEPS] | [] |
11,476 | RAD-sequencing | 1 | null | https://www.protocols.io/view/rad-sequencing-pfudjnw | Tomasz Suchan | TITLE: RAD-sequencing
AUTHORS: Tomasz Suchan
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Preparation]
Prepare reagents
?. [Restriction reaction]
Prepare the restriction reaction mix for all the samples using the amounts of reagents below:
[nuclease-free water]
[CutSmart buffer (10x)]
[MseI (10,000 U/m... | ["[Preparation]\nPrepare reagents", "[Restriction reaction]\nPrepare the restriction reaction mix for all the samples using the amounts of reagents below:\n[nuclease-free water]\n[CutSmart buffer (10x)]\n[MseI (10,000 U/ml)]\n[SbfI (HF) (20,000 U/ml)]", "[Ligation reaction]\nCombine 9 uL of the restriction reaction pro... |
28,637 | SPS-PAGE protein electrophoresis | null | dx.doi.org/10.17504/protocols.io.775hrq6 | null | Konstantinos Ragios | TITLE: SPS-PAGE protein electrophoresis
AUTHORS: Konstantinos Ragios
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of PAGE that allows to separate protein molecules according to their molecular masses under electric ... | ["[Sample preparation]\nIn a tube add 10μl of 2x Laemmli Buffer , 5μl Nuclease free water and 5μl of your protein sample.\nYou may adjust the ratio of water:protein depending on the concentration of proteins in your sample.e.g. for high concentration add 7μl of water and 3μl of proteins for low concentration add... |
90,823 | Multiple Sequence Alignment | 1 | dx.doi.org/10.17504/protocols.io.eq2lyj14wlx9/v1 | https://www.protocols.io/view/multiple-sequence-alignment-c4xfyxjn | Leonardo A Parra-Rivas | TITLE: Multiple Sequence Alignment
AUTHORS: Leonardo A Parra-Rivas
[DESCRIPTION]
Multiple Sequence Alignment
[STEPS]
1. Protein sequences were retrieved from Uniprot or NCBI via Jalview’s sequence fetcher Jalview
(RRID:SCR_006459)(https://www.jalview.org/help/html/features/seqfetch.html).
2. Then, the sequence... | ["Protein sequences were retrieved from Uniprot or NCBI via Jalview’s sequence fetcher Jalview\n(RRID:SCR_006459)(https://www.jalview.org/help/html/features/seqfetch.html).", "Then, the sequences were aligned with the\nClustal Omega Webserver [(RRID:SCR_001591) http://www.ebi.ac.uk/Tools/msa/clustalo/]\nwith default pa... |
54,372 | Agarose gel electrophoresis | 1 | null | https://www.protocols.io/view/agarose-gel-electrophoresis-bzccp2sw | Yuichiroh Ikagawa | TITLE: Agarose gel electrophoresis
AUTHORS: Yuichiroh Ikagawa
[DESCRIPTION]
Electrophoresis is the most basic technique for identifying DNA amplified by PCR or restriction enzyme treatment. Agarose gels are used to separate the DNA by molecular weight. Here we describe the technique using a 1.5 % agarose gel.
[STEPS... | ["Weigh out 0.75 g of agarose powder and add it to the triangular flask.", "Add 49 ml of sterile water to the first flask and shake gently.", "Add 1 ml x50 TAE buffer to the flask and heat in the microwave.", "Allow the melted agarose to cool for 5 minutes before pouring it into a template set with a comb.", "Cover wit... |
58,032 | Building a SpikeGLX Rig with camera: Chronic recoverable Neuropixels in mice | 1 | null | https://www.protocols.io/view/building-a-spikeglx-rig-with-camera-chronic-recove-b4wqqxdw | Emily A Aery Jones | TITLE: Building a SpikeGLX Rig with camera: Chronic recoverable Neuropixels in mice
AUTHORS: Emily A Aery Jones
[DESCRIPTION]
This protocol collection explains how to build a low-cost, lightweight system to implant Neuropixels 1.0 probes into mice, record during freely moving behavior, then recover the probe for futur... | ["[Set up acquisition hardware] Load the PCIe module into your computer", "[Set up acquisition hardware] Load the PXIe modules and the IMEC module into the NIDAQ chassis", "[Set up acquisition hardware] Connect the PXIe modules to the BNC breakout board and the computer. Connect the IMEC SMA to the BNC board (analog sl... |
34,330 | Protein expression and extraction of hard-to-produce proteins in the periplasmic space of Escherichia coli | 1 | dx.doi.org/10.17504/protocols.io.bdr2i58e | https://www.protocols.io/view/protein-expression-and-extraction-of-hard-to-produ-bdr2i58e | Cristina Hernandez Rollan, Kristoffer Bach Falkenberg, Maja Rennig, Andreas Birk Bertelsen, Morten Norholm | TITLE: Protein expression and extraction of hard-to-produce proteins in the periplasmic space of Escherichia coli
AUTHORS: Cristina Hernandez Rollan, Kristoffer Bach Falkenberg, Maja Rennig, Andreas Birk Bertelsen, Morten Norholm
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-st... | ["[Pre culture - Day 1]\nPick a fresh colony of your BL21 (DE3) strain with your expression vector, and inoculate it in LB supplemented with relevant antibiotics. Grow the culture at at 250 RPM shaking . The volume of the overnight culture depends on the volume of the expression culture and should be at least 1/100 of... |
54,418 | SNARE-seq2 | 1 | dx.doi.org/10.17504/protocols.io.bzdsp26e | https://www.protocols.io/view/snare-seq2-bzdsp26e | Nongluk Plongthongkum, Dinh H Diep, Song Chen, Blue Lake, Kun Zhang | TITLE: SNARE-seq2
AUTHORS: Nongluk Plongthongkum, Dinh H Diep, Song Chen, Blue Lake, Kun Zhang
[DESCRIPTION]
To study the heterogeneity of complex tissues by joint profiling of gene expression and its regulation, we require an accurate and high-throughput method. Here we described improved high-throughput combinato... | ["[Reagent setup] 40% (wt/vol) PEG 6000. Weigh 16.0 g of PEG 6000 in 50 mL tube. Add nuclease-free water and bring the total volume to 40 mL. Rotate the tube at room temperature until PEG 6000 completely dissolved. Spin down the tube at 200 g for 2 min, at room temperature to remove the tiny bubble. CRITICAL: 40% (wt/v... |
64,576 | Latest Pakistan News | 1 | dx.doi.org/10.17504/protocols.io.5jyl898m8v2w/v1 | https://www.protocols.io/view/latest-pakistan-news-cba8sihw | Tamara Walsky, Ariana Macon | TITLE: Latest Pakistan News
AUTHORS: Tamara Walsky, Ariana Macon
[DESCRIPTION]
Please provide an abstract for your protocol before publication.
[STEPS]
1. Latest Pakistan News Nation celebrates victory in 1971 independence war by hosting military parade with India’s president as visitor of honour.... | ["Latest Pakistan News Nation celebrates victory in 1971 independence war by hosting military parade with India’s president as visitor of honour.\n\n Qatil Haseenaon Ke Naam has femme fatales in saris, jhumka earrings as Professionaltagonists and may be streamed in one hundred ninety nations.... |
92,196 | Protein aggregation capture (PAC) and minimal automated processing for proteomics using magnetic beads and a Beckman Biomek™ NxP workstation for 96 well plates. | 1 | dx.doi.org/10.17504/protocols.io.bp2l6xz4rlqe/v1 | https://www.protocols.io/view/protein-aggregation-capture-pac-and-minimal-autom-c6aczaaw | ronan.ocualain | TITLE: Protein aggregation capture (PAC) and minimal automated processing for proteomics using magnetic beads and a Beckman Biomek™ NxP workstation for 96 well plates.
AUTHORS: ronan.ocualain
[DESCRIPTION]
Sample preparation for mass spectrometry analysis involves numerous liquid transfer steps.
These include
sa... | ["[Bead preparation] Briefly vortex the beads and place the tube on a magnetic stand for 2 min to collect the beads.", "[Bead preparation] Add ultrapure water at a volume corresponding to 5 to 10 times the initial volume of mixed beads.\nVortex the beads for 10 s and place on a magnetic stand for 2 min to collect the b... |
100,582 | Setting up Zotero and Google Drive for syncing reference libraries (PC) | 1 | dx.doi.org/10.17504/protocols.io.5jyl8jwprg2w/v2 | https://www.protocols.io/view/setting-up-zotero-and-google-drive-for-syncing-ref-dege3bte | Carie M. Frantz | TITLE: Setting up Zotero and Google Drive for syncing reference libraries (PC)
AUTHORS: Carie M. Frantz
[DESCRIPTION]
This protocol is useful if you wish to sync a LARGE reference library across multiple computers. It uses Zotero, which has built-in free syncing for up to 300 MB of files (roughly 50-100 papers). For l... | ["[Set up Google Drive syncing] Install Drive for desktop on both computers", "[Install and set up Zotero on Computer 1] Install Zotero desktop\n\nDefault installation settings are fine.", "[Install and set up Zotero on Computer 2] Repeat the steps above for your second computer\n\nIf you do not have a prior (e.g., Men... |
25,035 | Thawing of human pluripotent stem cells | null | dx.doi.org/10.17504/protocols.io.4pjgvkn | null | Ralitsa Madsen | TITLE: Thawing of human pluripotent stem cells
AUTHORS: Ralitsa Madsen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the thawing of human pluripotent stem cells. </div></div>
[STEPS]
?. If the cells were frozen from 1/2 or 1/3 of a subconfluent (80-90 %) 6-well plate, ... | ["If the cells were frozen from 1/2 or 1/3 of a subconfluent (80-90 %) 6-well plate, they will need to be thawed into 1 or max 2 wells of a Geltrex-coated 6-well plate (number of wells might require empirical optimisation based on knowledge about the particular cell line's behaviour). Therefore prepare the required amo... |
89,421 | Masson-Fontana Staining | 4 | dx.doi.org/10.17504/protocols.io.ewov1q3opgr2/v1 | https://www.protocols.io/view/masson-fontana-staining-c3jmykk6 | Nuriapenuelas | TITLE: Masson-Fontana Staining
AUTHORS: Nuriapenuelas
[DESCRIPTION]
Masson-Fontana Staining for melanin detection
[STEPS]
1. Heat paraffin tissue sections at 60ºC for 10min.
2. Incubate sections in xylene (5 min, 3 times) and in ethanol serial washes (100-95-70%-H2O, 5 min each).
4. Incubate the sections with sodium... | ["Heat paraffin tissue sections at 60ºC for 10min.", "Incubate sections in xylene (5 min, 3 times) and in ethanol serial washes (100-95-70%-H2O, 5 min each).", "Incubate the sections with sodium thiosulphate for 2 min. Rinse sections in distilled water.", "Incubate the sections with ammoniac solution for 40 min at 56ºC... |
54,061 | Guidance for populating GenomeTrakr metadata templates (BioSample and SRA) | 1 | dx.doi.org/10.17504/protocols.io.by2mpyc6 | https://www.protocols.io/view/guidance-for-populating-genometrakr-metadata-templ-by2mpyc6 | Ruth Timme, Maria Balkey, William Wolfgang, Errol Strain | TITLE: Guidance for populating GenomeTrakr metadata templates (BioSample and SRA)
AUTHORS: Ruth Timme, Maria Balkey, William Wolfgang, Errol Strain
[DESCRIPTION]
PURPOSE: Guidance on how to populate NCBI's metadata packages, maximizing interoperability for foodborne pathogen surveillance.
SCOPE: This protocol p... | ["[Overview] Guidance for organizing and populating the metadata templates required for direct submission to NCBI. This guidance is applicable for most enterics and/or microbial pathogens. \n\n****If your laboratory uses the BioNumerics platform for submission, please follow this protocol.****\n\nTwo metadata template... |
51,140 | IMC STAINING FOR PARAFFIN SECTIONS | 4 | dx.doi.org/10.17504/protocols.io.bv7cn9iw | https://www.protocols.io/view/imc-staining-for-paraffin-sections-bv7cn9iw | Klaus H. Kaestner Lab, Suzanne Shapira | TITLE: IMC STAINING FOR PARAFFIN SECTIONS
AUTHORS: Klaus H. Kaestner Lab, Suzanne Shapira
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Imaging mass cytometry (IMC) is a multiplexed imaging method that allows samples to be probed with up to forty antibodies at on... | ["[Procedure]\nUse pressure cooker or heat block to preheat Antigen Retrieval Solution to before starting.\n95 °C", "[Procedure]\nBake slides in over for 20 min.\n56 °C", "[Procedure]\nDewax slides in xylene in the fume hood for 5 min X 2.", "[Procedure]\nHydrate slides in descending grades of ethanol (100%, 95%, 80%... |
17,443 | Cryptococcus neoformans DNA Extraction Method | 1 | dx.doi.org/10.17504/protocols.io.4r3l2522jl1y/v1 | https://www.protocols.click/view/cryptococcus-neoformans-dna-extraction-method-vabe2an | Amy Gladfelter | TITLE: Cryptococcus neoformans DNA Extraction Method
AUTHORS: Amy Gladfelter
[DESCRIPTION]
Extraction method to obtain genomic DNA from Cryptococcus neoformans.
[GUIDELINES]
Extraction buffer (100 ml)
CTAB is mixed alkyltrimethyl ammonium bromide, Sigma cat.# M7635. This takes time to go into solution. You can ... | ["Grow a 50 mL YPD culture overnight (960 min), shaking at 30 °C.", "Pellet cells in tabletop centrifuge in a 50 ml disposable tube.", "Freeze cells at -20 °Cto -80 °C for <30 min, then dry in a freeze drying machine.", "Add the equivalent of 3 mL to 5 mLof 2 mm glass beads and vortex/shake until the cell pellet is bro... |
74,104 | Protocol for the double extraction of starch grains and phytoliths from sediments, V.1 | 1 | dx.doi.org/10.17504/protocols.io.5jyl8jdq8g2w/v1 | https://www.protocols.io/view/protocol-for-the-double-extraction-of-starch-grain-ckkyuuxw | Carlos G. Santiago-Marrero, Jaime R. Pagán-Jiménez | TITLE: Protocol for the double extraction of starch grains and phytoliths from sediments, V.1
AUTHORS: Carlos G. Santiago-Marrero, Jaime R. Pagán-Jiménez
[DESCRIPTION]
Most of the protocols employed for the separation of microbotanical remains from sediments follow the same principles: (1) preparing the sediment sampl... | ["[Clays deflocculation] Take a labelled and previously weighed new 50 mL sterile tube and carefully add the corresponding sample to be processed. Cover the mouth of the tube with aluminium foil and poke small holes (Figure 4). This will allow the samples to dry and minimise the chance for airborne contaminants to ente... |
null | null | null | dx.doi.org/10.17504/protocols.io.qn6dvhe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This year´s interlab study allows participants to fulfil Bronze medal requirements. </p>
<p>For this purposes a set of experiments has to be performed, which in the end will be compared and validated with other team´s data. </p>
<p>Part of this Challenge are the Calibration p... | [] |
82,703 | Cellular lipid uptake with flow cytometry readout | 4 | dx.doi.org/10.17504/protocols.io.14egn2pnpg5d/v1 | https://www.protocols.click/view/cellular-lipid-uptake-with-flow-cytometry-readout-cuzpwx5n | Igor Beletchi, rosanne.wouters, Peter Vangheluwe | TITLE: Cellular lipid uptake with flow cytometry readout
AUTHORS: Igor Beletchi, rosanne.wouters, Peter Vangheluwe
[DESCRIPTION]
Cellular lipid uptake with flow cytometry readout
[STEPS]
SECTION: harvest cells
1. harvest cells by detachment with
SECTION: harvest cells
1.1. cells should be grown to 70% confluency, ... | ["[harvest cells] harvest cells by detachment with", "[harvest cells] cells should be grown to 70% confluency, not higher", "[harvest cells] collect cells by centrifugation 400 x g, 5 min, 4 °C", "[harvest cells] wash pellet with with", "[harvest cells] collect cells by centrifugation 400 x g, 5 min, 4 °C", "[harvest c... |
65,717 | Primal Grow Pro Reviews : {Canada, Australia, United States & United Kingdom} [Available Now] | 1 | dx.doi.org/10.17504/protocols.io.3byl4by7jvo5/v1 | https://www.protocols.io/view/primal-grow-pro-reviews-canada-australia-united-st-ccevste6 | jatkima | TITLE: Primal Grow Pro Reviews : {Canada, Australia, United States & United Kingdom} [Available Now]
AUTHORS: jatkima
[DESCRIPTION]
• Official Website : Click Here
• Introducing: Primal Grow Pro Reviews
• STEP-BY-STEP Effects of the Ingredients: Primal Grow Pro Reviews
• Ingredients: Primal Grow Pro Reviews
... | [] |
40,790 | ELISA for quantification of IL-27 in human serum. | 6 | dx.doi.org/10.17504/protocols.io.bj3wkqpe | https://www.protocols.io/view/elisa-for-quantification-of-il-27-in-human-serum-bj3wkqpe | Angel Justiz-Vaillant | TITLE: ELISA for quantification of IL-27 in human serum.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. ... | ["An anti-human IL-27 coating antibody is adsorbed onto the microwells by incubation overnight at 4°C with carbonate-bicarbonate buffer.", "Add 50 µl of human serum. Human IL-27 present in the serum sample binds to antibodies adsorbed into the microwells.", "The microplate is blocked with 3% non-fat milk-PBS buffer and... |
23,886 | Neuropathy Phentoyping Protocols - Basic Breeding to Harvest | null | dx.doi.org/10.17504/protocols.io.3jngkme | null | Eva Feldman | TITLE: Neuropathy Phentoyping Protocols - Basic Breeding to Harvest
AUTHORS: Eva Feldman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block"><span style = "font-weight:bold;">Phenotyping of Rodents for the Presence of Diabe... | ["Basic breeding scheme is harem breeding: 2 females, 1 male per cage.", "At least 3 breeding trios are active and are replaced every 6 months.", "Tail biopsies are collect at 3-4 weeks of age for genotyping.", "Fasting blood glucose tests are performed every 4 weeks.", "Body weights are recorded every 4 weeks at same ... |
null | null | null | dx.doi.org/10.17504/protocols.io.etwbepe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This medium is for growing <em>Chlorella variabilis</em> NC64A, <em>Chlorella heliozoae</em> SAG 3.83, and <em>Coccomyxa</em> C-169.
[GUIDELINES]
<p><strong>BBM Stock Solutions</strong></p>
<ol>
<li> 25.0 gm NaNO<sub>3</sub> per liter d-H<sub>2</sub>O</li>
<li> 2.5 gm CaCl<sub>... | [] |
74,548 | Determination of extracellular polymeric substances (EPS) using a modified phenol-sulfuric acid (PSA) assay for sugars | 6 | dx.doi.org/10.17504/protocols.io.bp2l69871lqe/v1 | https://www.protocols.io/view/determination-of-extracellular-polymeric-substance-ck2uuyew | Jody Deming, Shelly Carpenter | TITLE: Determination of extracellular polymeric substances (EPS) using a modified phenol-sulfuric acid (PSA) assay for sugars
AUTHORS: Jody Deming, Shelly Carpenter
[DESCRIPTION]
This described protocol reflects our experience in measuring extracellular polysaccharide substances (EPS), particulate and dissolved, in ve... | ["[Subsampling for EPS] Using a DI-rinsed graduated cylinder, sub-sample 300–500 ml of the sample of interest (reduce the volume if a high biomass is known or suspected, as the filter will clog). Record the volume filtered. (Marine examples: for seawater, > 600 ml is typical; for melted bottom sea ice, 25 ml may be ade... |
null | null | null | dx.doi.org/10.17504/protocols.io.ga4bsgw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is a modified protocol for extracting DNA from fungal myelium using the Sigma-Aldrich Extract-N-Amp Plant Kit published by J.M. U'Ren et al. (2016) Contributions of North American endophytes to the phylogeny, ecology, and taxonomy of Xylariaceae (Sordariomycetes, Ascomyc... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.dp65rd | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Another convenient way to screen larger sample volumes is to perform liquid enrichment cultures (Suttle 1993). By this approach, larger volumes of water samples can be screened for cyanophages, thus enabling detection of “rare” viruses. Similar to the use of enrichment cultures ... | [] |
52,725 | Microscopy-based bead protein-protein interaction assay | 4 | dx.doi.org/10.17504/protocols.io.bxqvpmw6 | https://www.protocols.io/view/microscopy-based-bead-protein-protein-interaction-bxqvpmw6 | Chunmei Chang | TITLE: Microscopy-based bead protein-protein interaction assay
AUTHORS: Chunmei Chang
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">An assay to study protein-protein interaction</div></div>
[STEPS]
?. Wash glutathione sepharose beads (GE Healthcare) with the reaction buffer (20 mM HEPES at pH 8.... | ["Wash glutathione sepharose beads (GE Healthcare) with the reaction buffer (20 mM HEPES at pH 8.0, 200 mM NaCl and 1 mM TCEP) three times.", "Make a mixture of 0.5 µM GST or GST tagged cargo receptors and different ATG proteins.", "Incubate the protein mixture with 10 µL washed glutathione sepharose beads at room tem... |
44,898 | Protocols for study of "Hippocampal transcriptome-wide association study and neurobiological pathway analysis for Alzheimer's disease" | 1 | dx.doi.org/10.17504/protocols.io.bp4amqse | https://www.protocols.io/view/protocols-for-study-of-34-hippocampal-transcriptom-bp4amqse | Nana Liu, Jiayuan Xu, Huaigui Liu, Shijie Zhang, Miaoxin Li, Yao Zhou, Wen Qin, Mulin Jun Li, Chunshui Yu | TITLE: Protocols for study of "Hippocampal transcriptome-wide association study and neurobiological pathway analysis for Alzheimer's disease"
AUTHORS: Nana Liu, Jiayuan Xu, Huaigui Liu, Shijie Zhang, Miaoxin Li, Yao Zhou, Wen Qin, Mulin Jun Li, Chunshui Yu
[STEPS]
?. Step1. Quality control (QC) and imputation for ... | ["Step1. Quality control (QC) and imputation for genotype data from GTEx version7", "Pre-imputation QCTools: PLINK (https://www.cog-genomics.org/plink/) [1]The sample-level QC: a. Genotyping call rate per individual (> 98%)plink --bfile ${genotype_data} --missing --out ${missingness}awk '{if($6 > 0.02)print$0}' ${m... |
44,656 | German Glühwein and Kinderpunsch | 1 | null | https://www.protocols.io/view/german-gl-hwein-and-kinderpunsch-bpuqmnvw | Anita Broellochs | TITLE: German Glühwein and Kinderpunsch
AUTHORS: Anita Broellochs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Glühwein (Mulled wine) is a hot spiced wine beverage which is a traditional drink during the winter in Germany and is served at Christmas markets in Europe. The non-alcoholic version is ... | ["Please choose between: 1. Glühwein - alcohol containing 2. Kinderpunsch - non-alcoholic", "Pour the into a medium sized pot.\n[bottle of dry red wine]", "Add the following ingredients to the pot: Optional:\n[cinnamon sticks]\n[whole star anise]\n[whole cloves]\n[capsules of Cardamom]\n[sliced organic orange (with pe... |
54,947 | Mapeamento de literatura sobre mulher preta na Ciência da Informação no Brasil | 3 | dx.doi.org/10.17504/protocols.io.bzwbp7an | https://www.protocols.io/view/mapeamento-de-literatura-sobre-mulher-preta-na-ci-bzwbp7an | Gabriele Rodrigues Pereira, Zaira Regina Zafalon | TITLE: Mapeamento de literatura sobre mulher preta na Ciência da Informação no Brasil
AUTHORS: Gabriele Rodrigues Pereira, Zaira Regina Zafalon
[DESCRIPTION]
Pesquisa sobre identificação de estudos sobre mulher preta na Ciência da Informação no Brasil, desenvolvida por Gabriele Rodrigues Pereira como parte da pesqui... | [] |
109,187 | Protocols for stereotaxic injections into mouse brain and ex-vivo electrophysiology | 0 | dx.doi.org/10.17504/protocols.io.n2bvjn7qpgk5/v1 | https://www.protocols.io/view/protocols-for-stereotaxic-injections-into-mouse-br-dnvb5e2n | Jyoti Gupta, Michael J. Higley | TITLE: Protocols for stereotaxic injections into mouse brain and ex-vivo electrophysiology
AUTHORS: Jyoti Gupta, Michael J. Higley
[DESCRIPTION]
This protocol describes the method for injection of α-Synuclin PFF and monomer into the mouse brain. The second part of the protocol describes preparation of acute slices fro... | ["[Protocol for stereotaxic injections] Anesthetize the mouse with isoflurane by placing in the anesthesia chamber.", "[Protocol for stereotaxic injections] Place the anesthetized mouse in the stereotaxic frame and continually maintain isoflurane anesthesia using a vaporizer (Harvard Apparatus).", "[Protocol for stereo... |
76,756 | Multimodal imaging pipeline for molecular and cellular characterization of Liver tissue - HuBMAP TTD-Columbia/PSU | 1 | dx.doi.org/10.17504/protocols.io.kxygx913zg8j/v1 | https://www.protocols.io/view/multimodal-imaging-pipeline-for-molecular-and-cell-cn7uvhnw | Presha Rajbhandari, Hua Tian, Aubrianna Decker, Taruna Neelakantan, Brent R Stockwell | TITLE: Multimodal imaging pipeline for molecular and cellular characterization of Liver tissue - HuBMAP TTD-Columbia/PSU
AUTHORS: Presha Rajbhandari, Hua Tian, Aubrianna Decker, Taruna Neelakantan, Brent R Stockwell
[DESCRIPTION]
We have developed a workflow to assess the spatial organization of liver tissue with re... | ["Acquire human tissue blocks.\ndx.doi.org/10.17504/protocols.io.rm7vzy5wrlx1/v1", "Prepare tissue sections for multimodal imaging on appropriate substrates.\ndx.doi.org/10.17504/protocols.io.e6nvwjmjzlmk/v1", "H&E staining is performed using standard protocol.", "DESI-IMS is performed as described here.\ndx.doi.org/10... |
38,849 | Optimized HT gDNA extraction from Dried Blood Spot using QIAcube HT for Malaria Genomic Epidemiology Studies | 4 | dx.doi.org/10.17504/protocols.io.bh69j9h6 | https://www.protocols.io/view/optimized-ht-gdna-extraction-from-dried-blood-spot-bh69j9h6 | Abebe A. Fola , Jack Dorman, Maria Levy, Ilinca Ciubotariu, Giovanna Carpi | TITLE: Optimized HT gDNA extraction from Dried Blood Spot using QIAcube HT for Malaria Genomic Epidemiology Studies
AUTHORS: Abebe A. Fola , Jack Dorman, Maria Levy, Ilinca Ciubotariu, Giovanna Carpi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><sp... | ["[DBS Pretreatment]\nLabel a new S-Block with important information such as date, project name, sample type, etc. Use the same information to generate .csv file that contains sample ID and well position which will be imported to QIAcube HT robot.", "[DBS Pretreatment]\nUsing clean tweezers, remove a DBS sample from it... |
68,735 | Sample Preparation of Human Eye Anterior Segment for sc/snRNA-seq | 1 | dx.doi.org/10.17504/protocols.io.n92ldzo8ov5b/v1 | https://www.protocols.io/view/sample-preparation-of-human-eye-anterior-segment-f-cfc7tizn | Xuan Bao, Xuesen Cheng, Yumei Li, Rui Chen | TITLE: Sample Preparation of Human Eye Anterior Segment for sc/snRNA-seq
AUTHORS: Xuan Bao, Xuesen Cheng, Yumei Li, Rui Chen
[DESCRIPTION]
This protocol is used for sample preparation of human donor eye for single cell/single nuclei RNA sequencing. The protocol consists of dissection, fresh and frozen tissue dissociat... | ["[Dissection of Human Donor Eye] Recovery Conditions\nFor a good quality of retina and RPE, eyes should be recovered no more than 6 hours post-mortem. If more than 6 hours but less than 12 hours, eyes could still be recovered and dissected, but posterior dissection for single cell/nuclei can be omitted (part 2.3). It... |
83,740 | Genetic Characterization of Schistosomes | 4 | dx.doi.org/10.17504/protocols.io.5qpvo3nobv4o/v1 | https://www.protocols.click/view/genetic-characterization-of-schistosomes-cvz4w78w | Frank Twum Aboagye, Isaac Owusu-Frimpong, Naa A. Kuma, Samuel .K. Armoo, Mike Y. Osei-Atweneboana, Yvonne A. Ashong | TITLE: Genetic Characterization of Schistosomes
AUTHORS: Frank Twum Aboagye, Isaac Owusu-Frimpong, Naa A. Kuma, Samuel .K. Armoo, Mike Y. Osei-Atweneboana, Yvonne A. Ashong
[DESCRIPTION]
Schistosomiasis is a parasitic disease transmitted through water by blood-fluke trematodes of the genus Schistosoma. After malaria a... | ["[Nucleic Acid Isolation] Transfer 200 µL of the pre-treated sample (cercariae suspension or snail) into a sterile 1.5 mL microcentrifuge tube.", "[Nucleic Acid Isolation] Add 200 µL of 2 Mass / % volume PVPP in 1XPBS and vortex the sample with glass beads at for 1 min", "[Nucleic Acid Isolation] Add 400 µL of Genom... |
97,127 | Nanopore SISPA | 0 | null | https://www.protocols.io/view/nanopore-sispa-da4f2gtn | Siu Fung Ho | TITLE: Nanopore SISPA
AUTHORS: Siu Fung Ho
[DESCRIPTION]
Nanopore SISPA
[STEPS]
SECTION: RNA extraction with QIAamp Viral RNA Mini Kit
3. Add 1:500 linear acrylamide instead of carrier RNA to Buffer AVL.
SECTION: Sample Preprocessing
2. Use 280 μl of the filtrate as starting material for the Qiagen Viral RNA Mini Ki... | ["[RNA extraction with QIAamp Viral RNA Mini Kit] Add 1:500 linear acrylamide instead of carrier RNA to Buffer AVL.", "[Sample Preprocessing] Use 280 μl of the filtrate as starting material for the Qiagen Viral RNA Mini Kit.", "[RNA extraction with QIAamp Viral RNA Mini Kit] Pipet 1120 μL prepared Buffer AVL into a 2 m... |
51,089 | Ex vivo generation and maintenance of human Epstein-Barr Virus (EBV)-specific T cells lacking alloreactivity | 4 | dx.doi.org/10.17504/protocols.io.bv5rn856 | https://www.protocols.io/view/ex-vivo-generation-and-maintenance-of-human-epstei-bv5rn856 | Zaki Molvi | TITLE: Ex vivo generation and maintenance of human Epstein-Barr Virus (EBV)-specific T cells lacking alloreactivity
AUTHORS: Zaki Molvi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">T cells recognizing Epstein-Barr Virus (EBV) can be generated ex vivo from most immunocompetent human subjects and m... | ["[Materials]\nHuman PBMCEBV B95-8 transformed B cells (BLCL) from the same donor as the PBMC (protocol here)Human IL-2 T cell media: specialty medium (Yssel's, Xvivo-15 or -20, AIM-V, OpTimizer, CellGro DC media) supplemented with 5-10% human AB serum and 2mM L-Glutamine OR basal medium (RPMI 1640, IMDM) supplemented ... |
55,602 | Liposome Encapsulation of Hydrophilic and Hydrophobic Drugs | 1 | dx.doi.org/10.17504/protocols.io.rm7vzymorlx1/v1 | https://www.protocols.io/view/liposome-encapsulation-of-hydrophilic-and-hydropho-b2isqcee | Aisling Field, James F Curtin, Janith Wanigasekara, Julie Rose Mae Mondala | TITLE: Liposome Encapsulation of Hydrophilic and Hydrophobic Drugs
AUTHORS: Aisling Field, James F Curtin, Janith Wanigasekara, Julie Rose Mae Mondala
[DESCRIPTION]
Dried reconstituted vesicles (DRV) are liposomes that are produced under mild conditions and can encapsulate very high amounts of hydrophilic solutes. T... | ["[Liposome Encapsulation] Stir the mixture for 15 min at above Tc of the lipid (60 ⁰C).", "[Liposome Encapsulation] Dissolve 7 mmol of the lipid (DSPC), 3 mmol of cholesterol and 1:20 (w/w) Ursolic acid (lipophilic compound) in 5 ml chloroform in a round bottom flask.", "[Liposome Encapsulation] Dry the lipid using a ... |
101,527 | Installing FIJI and SynBot (Mac Version) | 0 | dx.doi.org/10.17504/protocols.io.14egn6936l5d/v1 | https://www.protocols.io/view/installing-fiji-and-synbot-mac-version-dfdx3i7n | Justin T Savage | TITLE: Installing FIJI and SynBot (Mac Version)
AUTHORS: Justin T Savage
[DESCRIPTION]
Video instructions for installing FIJI and the software needed for SynBot on MacOS.
[STEPS]
1.
SECTION: Install SynBot
4. 1:11-1:56 Download code from the SynBot GitHub repository https://github.com/Eroglu-Lab/Syn_Bot .
SECTION... | ["[Install SynBot] 1:11-1:56 Download code from the SynBot GitHub repository https://github.com/Eroglu-Lab/Syn_Bot .", "[Install FIJI] 0:30-1:11 Give Mac permission to open FIJI since it is from an outside developer.", "[Install FIJI] 0:00-0:30 Download FIJI from https://fiji.sc .", "[Install SynBot] 1:56-2:39 Move Syn... |
86,535 | Dopamine neuron enrichment using MACS | 1 | dx.doi.org/10.17504/protocols.io.n92ldmmpnl5b/v1 | https://www.protocols.io/view/dopamine-neuron-enrichment-using-macs-cyrfxv3n | Tae Wan Kim | TITLE: Dopamine neuron enrichment using MACS
AUTHORS: Tae Wan Kim
[DESCRIPTION]
high yield DA neuron sorting via MACS but using surface marker strategy described recently (Kim et al.,BioRxiv).
[STEPS]
SECTION: Cell dissociation
1. Use Worthington Papain Dissociation System (LK003150) to dissociate at day 25 of Stud... | ["[Cell dissociation] Use Worthington Papain Dissociation System (LK003150) to dissociate at day 25 of Studer lab dopamine neuron protocol. Add 32 mls of EBSS (vial 1) to the albumin ovomucoid inhibitor mixture (vial 4) and allow the contents to dissolve while preparing the other components. Mix before using and equili... |
68,407 | IB BIO SL IA M23 - A study on the conditions that affect yeast fermentation | 4 | dx.doi.org/10.17504/protocols.io.261geny6jg47/v2 | https://www.protocols.io/view/ib-bio-sl-ia-m23-a-study-on-the-conditions-that-af-ce2xtgfn | Marcy Roca Cugat | TITLE: IB BIO SL IA M23 - A study on the conditions that affect yeast fermentation
AUTHORS: Marcy Roca Cugat
[DESCRIPTION]
This protocol is designed to aid me in the research for my International Baccalaureate Biology Internal Assignment
[GUIDELINES]
None
[STEPS]
SECTION: Preparation
1. Check that all reagents and ... | ["[Preparation] Check that all reagents and materials are ready.", "[Preparation] Assemble the instrumentation", "[Preparation] One end of the flask must be plugged with a holed stopper and a thermometer.\nThe other end should be plugged with a holed stopper and a glass tube.\nThe middle opening should be covered with ... |
47,971 | DNA Extraction from Modern Dental Plaque on Gauze | 1 | dx.doi.org/10.17504/protocols.io.eq2lypejrlx9/v1 | https://www.protocols.io/view/dna-extraction-from-modern-dental-plaque-on-gauze-bs4bngsn | Franziska Aron, Irina Velsko | TITLE: DNA Extraction from Modern Dental Plaque on Gauze
AUTHORS: Franziska Aron, Irina Velsko
[DESCRIPTION]
Protocol for DNA extraction from modern dental plaque dried on gauze samples for Illumina sequencing. This protocol uses the Qiagen PowerSoil DNA extraction kit, but applies modifications to improve DNA reco... | ["[Preparation Day 1] Place a 1.5ml/2ml tube rack at 4 °C for the incubation steps on the final days", "[Preparation Day 1] Label for each sample one 2ml-Eppendorf Safe-Lock DNA LoBind-Tube\n and one 1.5ml-Eppendorf Safe-Lock DNA LoBind-Tube", "[Preparation for Final Day] Retrieve the spin filter columns (provided in t... |
66,359 | Le guide avancé de Detoxil Avis | 1 | dx.doi.org/10.17504/protocols.io.n92ldzxzov5b/v1 | https://www.protocols.io/view/le-guide-avanc-de-detoxil-avis-cc2xsyfn | Romanballor | TITLE: Le guide avancé de Detoxil Avis
AUTHORS: Romanballor
[DESCRIPTION]
Diaetoxil Avis: ne personne a besoin d'avoir un corps sain. Peu importe votre richesse financière ou toutes les ressources dont vous disposez, si votre santé est mauvaise, vous attirerez de nombreux problèmes de santé dans votre compete. Tous ... | ["[Le guide avancé de Detoxil Avis]"] |
100,230 | Labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy | 0 | dx.doi.org/10.17504/protocols.io.261ge5dpdg47/v1 | https://www.protocols.io/view/labeling-of-microtubules-using-mouse-anti-tubulin-dd5e283e | Steven Adams, Mason R. Mackey, Mark Ellisman | TITLE: Labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy
AUTHORS: Steven Adams, Mason R. Mackey, Mark Ellisman
[DESCRIPTION]
This protocol details th... | ["[Labeling of microtubules] Culture HEK293T cells on MatTek plates containing poly-D-lysine coated glass bottom No.0 coverslips in DMEM supplement with 10% fetal bovine serum.", "[Labeling of microtubules] Rinse the cells (x3) with cytoskeleton stabilizing buffer, at 37 °C and fixed with 4% paraformaldehyde (19202, E... |
34,837 | HubMap UF TMC Tissue Dissociation to Single Cell | null | dx.doi.org/10.17504/protocols.io.bd9vi966 | https://www.protocols.io/view/hubmap-uf-tmc-tissue-dissociation-to-single-cell-bd9vi966 | Marda Jorgensen, Maigan Brusko | TITLE: HubMap UF TMC Tissue Dissociation to Single Cell
AUTHORS: Marda Jorgensen, Maigan Brusko
[STEPS]
?. [Preparation]
To make ACK Lysis Buffer:
?. [Preparation]
Add 8.02 grams of NH4Cl, 0.84 grams of NaHCO, and 5uL of 0.5M EDTA into 1000mL of deionized water.
?. [Preparation]
Filter buffer through 22um PES Filter. ... | ["[Preparation]\nTo make ACK Lysis Buffer:", "[Preparation]\nAdd 8.02 grams of NH4Cl, 0.84 grams of NaHCO, and 5uL of 0.5M EDTA into 1000mL of deionized water.", "[Preparation]\nFilter buffer through 22um PES Filter. Aliquot in 50mL conicals and store at room temperature for long-term storage.", "[Preparation]\nTo make... |
92,583 | Colonic Migrating Motor Complexes | 1 | dx.doi.org/10.17504/protocols.io.n92ldm61nl5b/v1 | https://www.protocols.io/view/colonic-migrating-motor-complexes-c6nfzdbn | Jessica Griffiths, Bryan Yoo, Sarkis Mazmanian | TITLE: Colonic Migrating Motor Complexes
AUTHORS: Jessica Griffiths, Bryan Yoo, Sarkis Mazmanian
[DESCRIPTION]
Protocol used in "Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology"
[STEPS]
SECTION: Sample Preparation
1. Intact colons were dissected from cervically-dislocated mice.
SECTION... | ["[Sample Preparation] Intact colons were dissected from cervically-dislocated mice.", "[Sample Preparation] Colons were flushed and placed in pre-oxygenated (95% O2, 5% CO2)\nKrebs-Henseleit solution at RT", "[Sample Preparation] Proximal and distal ends were cannulated to 2 mm\ndiameter tubes and secured in the cente... |
36,885 | A method for isolating RNA from canine bone | null | dx.doi.org/10.17504/protocols.io.bf9vjr66 | https://www.protocols.io/view/a-method-for-isolating-rna-from-canine-bone-bf9vjr66 | Rebecca Nance, Payal Agarwal, Maninder Sandey, Dmytro Starenki, Jey Koehler, Abdul Mohin Sajib, Bruce F Smith | TITLE: A method for isolating RNA from canine bone
AUTHORS: Rebecca Nance, Payal Agarwal, Maninder Sandey, Dmytro Starenki, Jey Koehler, Abdul Mohin Sajib, Bruce F Smith
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Extracting sufficient quantity and quality RNA from bone is essential for downstr... | ["[Bone Preparation]\nRemove all exterior soft tissue around disphysis using scissors/scalpel/KimWipes. Use a scalpel to scrape bone along diaphysis exterior to ensure complete removal of periosteum.\n**Isolating RNA that is unique to osteoblasts, osteoclasts, and osteocytes is dependent on sufficient removal of these ... |
26,744 | Biohazard Garbage Protocol: Autoclave Biohazard Waste | null | dx.doi.org/10.17504/protocols.io.6cyhaxw | null | Steven Wilhelm, Gary LeCleir | TITLE: Biohazard Garbage Protocol: Autoclave Biohazard Waste
AUTHORS: Steven Wilhelm, Gary LeCleir
[STEPS]
?. Locate Biohazard garbage.
?. If the waste container is > ¾ full, then it needs to be processed for autoclaving.
?. Autoclave the processed waste on gravity cycle.
?. Instructions on cycle times based on the am... | ["Locate Biohazard garbage.", "If the waste container is > ¾ full, then it needs to be processed for autoclaving.", "Autoclave the processed waste on gravity cycle.", "Instructions on cycle times based on the amount of trash you are autoclaving are on the autoclave.", "Transfer bag into a wide autoclavable tray.\nTray ... |
63,893 | Krim Asli Artropant - (Hoax atau Sah) Benar-benar Berfungsi! | 3 | dx.doi.org/10.17504/protocols.io.ewov1nxrogr2/v1 | https://www.protocols.io/view/krim-asli-artropant-hoax-atau-sah-benar-benar-berf-camvsc66 | Artropant | TITLE: Krim Asli Artropant - (Hoax atau Sah) Benar-benar Berfungsi!
AUTHORS: Artropant
[DESCRIPTION]
Artropant adalah Orinial Cream yang membantu dalam pengurangan ketidaknyamanan sendi. beli krim alami harga terbaik periksa komposisi & manfaat, ulasan!
https://www.artropant.site/
https://www.agcef.org/artropant-asli... | [] |
57,834 | Development of a simple and versatile in vitro method for production, stimulation, and analysis of bioengineered muscle | 4 | dx.doi.org/10.17504/protocols.io.b4qiqvue | https://www.protocols.io/view/development-of-a-simple-and-versatile-in-vitro-met-b4qiqvue | Karen Wells-Cembrano, Júlia Sala, Jose Antonio del Rio | TITLE: Development of a simple and versatile in vitro method for production, stimulation, and analysis of bioengineered muscle
AUTHORS: Karen Wells-Cembrano, Júlia Sala, Jose Antonio del Rio
[DESCRIPTION]
In recent years, 3D in vitro modeling of human skeletal muscle has emerged as a subject of increasing interest, du... | ["[Making muscle culture devices] Mark a line of 1.2 cm in the center of each 35 mm ∅ Petri dish. In order to obtain consistently centered markings, it is recommended to make a small stencil out of paper or plastic (Figure 2).", "[Preparation of cells and materials] Defrost myoblast cell line in a T75 flask and culture... |
86,405 | Highway carbon unlocking efficiency study based on super-efficiency SBM-Malmquist | 1 | dx.doi.org/10.17504/protocols.io.8epv5xx7jg1b/v1 | https://www.protocols.io/view/highway-carbon-unlocking-efficiency-study-based-on-cymdxu26 | Yun Chen, Qi Luo, machongsen | TITLE: Highway carbon unlocking efficiency study based on super-efficiency SBM-Malmquist
AUTHORS: Yun Chen, Qi Luo, machongsen
[DESCRIPTION]
Carbon neutrality and sustainable development goals (SDGs), as new requirements for global development at this stage, have raised higher requirements for achieving the coordinati... | [] |
24,918 | Protocols for Image Capture and Data Recording in the Field | null | dx.doi.org/10.17504/protocols.io.4jwgupe | null | Richard Levy | TITLE: Protocols for Image Capture and Data Recording in the Field
AUTHORS: Richard Levy
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In effort to increase usefuleness of images that we capture in the field, Denver Botanic Gardens Research and Conservation has implemented this protocol to record ... | [] |
64,369 | Preparation of 1M magnesium sulfate solution (MgSO4) | 4 | dx.doi.org/10.17504/protocols.io.ewov1nmbygr2/v1 | https://www.protocols.io/view/preparation-of-1m-magnesium-sulfate-solution-mgso4-ca4rsgv6 | Alid Al-Asmar, Alfonso Pérez Escudero, gabrielmadirolas | TITLE: Preparation of 1M magnesium sulfate solution (MgSO4)
AUTHORS: Alid Al-Asmar, Alfonso Pérez Escudero, gabrielmadirolas
[DESCRIPTION]
Original source of the protocol: WormBook Methods http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html
This protocol is for making 1M magnesium sulfate sol... | ["Weigh 12.3 g of magnesium sulfate heptahydrate (MgSO4·7H2O), and add it to a 100 mL measuring cylinder.", "Add ~40 mL of milliQ water.", "Add a clean stirring magnet, and leave it on a stirrer until all crystals are dissolved.", "Remove the stirring magnet: To prevent touching the solution, use another magnet from th... |
57,739 | Duplicating 96-well plate-cultured hPSCs clones | 4 | dx.doi.org/10.17504/protocols.io.b4mjqu4n | https://www.protocols.io/view/duplicating-96-well-plate-cultured-hpscs-clones-b4mjqu4n | Hanqin Li, Yogendra Verma, Dirk Hockemeyer, Frank Soldner | TITLE: Duplicating 96-well plate-cultured hPSCs clones
AUTHORS: Hanqin Li, Yogendra Verma, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes a standard procedure for duplicating 96-well plate-cultured human pluripotent stem cells (hPSCs).
General notes:
1. Throughout this protocol, the term hPSC i... | ["Change medium to hPSCs medium + Rock inhibitor one day ahead of plate duplication.", "Prepare 11 ml DPBS, 3 ml Trypsin, 15 ml hPSCs medium + Rock inhibitor and 3 ml 2x Crude lysis buffer (with proteinase K) into separate reservoirs.", "Aspirate the medium from plates, wash by dispensing 100 µl DPBS per well using a M... |
null | null | null | dx.doi.org/10.17504/protocols.io.eahbab6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is based on Schleper et al. (1992) as modified by Stedman et al. (2003).<br /><br />This is a protocol from: <br /><br />Stedman, K. M., K. Porter, and M. L. Dyall-Smith. 2010. Chapter 6: The isolation of viruses infecting Archaea. Manual of Aquatic Viral Ecology. ... | [] |
53,873 | Full plate & spot test plaque assays + PFU/mL calc. - aerobic bacteria | 4 | dx.doi.org/10.17504/protocols.io.byurpwv6 | https://www.protocols.io/view/full-plate-amp-spot-test-plaque-assays-pfu-ml-calc-byurpwv6 | Torben Sølbeck Rasmussen | TITLE: Full plate & spot test plaque assays + PFU/mL calc. - aerobic bacteria
AUTHORS: Torben Sølbeck Rasmussen
[DESCRIPTION]
Protocol for plaque assays - either a full plate plaque assay that are more laborious but with high accuracy, or a spot test plaque assay that are for higher throughput but with less accur... | ["[Initial preparation of bacterial culture] Spread bacterial culture on an agar plate and incubate at required temperature until clear colonies appear.", "[Initial preparation of bacterial culture] Inoculate with a single colony of the bacteria to prepared media, incubate at required temperature, and wait until expone... |
52,668 | Structural analysis of the GPI glycan | 4 | dx.doi.org/10.17504/protocols.io.bxn4pmgw | https://www.protocols.io/view/structural-analysis-of-the-gpi-glycan-bxn4pmgw | Miyako Nakano †, Susana Sabido-Bozo †, Kouta Okazaki, Auxiliadora Aguilera-Romero, Sofia Rodriguez-Gallardo, Alejandro Cortes-Garcia, Sergio Lopez, Atsuko Ikeda, Kouichi Funato, Manuel Muñiz | TITLE: Structural analysis of the GPI glycan
AUTHORS: Miyako Nakano †, Susana Sabido-Bozo †, Kouta Okazaki, Auxiliadora Aguilera-Romero, Sofia Rodriguez-Gallardo, Alejandro Cortes-Garcia, Sergio Lopez, Atsuko Ikeda, Kouichi Funato, Manuel Muñiz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Glycosy... | ["[Yeast growth and culture]\nPick up the yeast cells expressing the plasma membrane GPI-AP Gas1-GFP from a frozen stock using a sterile toothpick, streak them on an SD medium without leucine (SD-LEU) agar plate and incubate them at 24 ºC for 2-3 days.", "[Yeast growth and culture]\nInoculate the yeast cells into 4 ml ... |
68,610 | Tissue Fixation Preparation | 4 | dx.doi.org/10.17504/protocols.io.6qpvr61ozvmk/v1 | https://www.protocols.io/view/tissue-fixation-preparation-ce9ath2e | Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill | TITLE: Tissue Fixation Preparation
AUTHORS: Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill
[DESCRIPTION]
This protocol describes fixation of tissue in preparation for pathology review to assess tissue quality.
P... | ["[4% PFA Tissue Fixation of Ovaries] Place each pre-weighed piece of tissue in either a 1.5mL Eppendorf tube or 1.5mL cryo tube.", "[4% PFA Tissue Fixation of Ovaries] Keep PFA tubes on ice. Transfer tissue specimen into a tube and refrigerate at 4 °C for 1440 min .", "[4% PFA Tissue Fixation of Ovaries] After 1440 m... |
51,751 | Total protein in microalgae: Pierce BCA protein assay | 4 | null | https://www.protocols.io/view/total-protein-in-microalgae-pierce-bca-protein-ass-bwsfpebn | Yingyu Hu, Zoe V. Finkel | TITLE: Total protein in microalgae: Pierce BCA protein assay
AUTHORS: Yingyu Hu, Zoe V. Finkel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Here we describe a protocol for extracting total protein from microalgae and quantifying total protein by Pierce BCA protein assay.</div><br/><div class = "t... | ["[Sample collection]\nCalculate the volume to obtain enough biomass for the assay:Minimum sampling volume (mL) = 750/(Chl-a_ug/L), if protein is extracted by 500 ul extraction buffer.", "[Sample collection]\nUse 5 inches Hg to filter microalgae samples onto polycarbonate filters (pore size varies).", "[Sample collecti... |
71,031 | Viral-mediated short-hairpin RNA knockdown | 4 | dx.doi.org/10.17504/protocols.io.q26g7y819gwz/v1 | https://www.protocols.io/view/viral-mediated-short-hairpin-rna-knockdown-chkxt4xn | Chuyu Chen, Loukia Parisiadou | TITLE: Viral-mediated short-hairpin RNA knockdown
AUTHORS: Chuyu Chen, Loukia Parisiadou
[DESCRIPTION]
Stereotaxic injection of viral vectors for gene knockdown experiments : The AAV vectors for knocking down the gene of interest were custom-made with Vector Biolabs using AAV-GFP-U6 vector for AAV1 packaging (Cat no, ... | ["[Just before the procedure] The following preparatory steps are followed:", "[Just before the procedure] Weigh the animals", "[Just before the procedure] Calibrate the David Kopf frame based on the manufacturer’s instructions.", "[Just before the procedure] Clean the Hamilton Syringe with 70% ethanol and distilled wa... |
null | null | null | dx.doi.org/10.17504/protocols.io.nc5day6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>DNA isolation from gram negative bacteria with chemical SDS lysis (Phenol) for sequencing on Nanopore. Fast low input cell culture (5ml) with max output (2-3 µg) and longer reads ( 30kb+) .</p>
[BEFORE_START]
<p>Prepare buffer ( taken from http://bit.ly/2FfjomS @Josh Quick)<... | [] |
85,791 | KAPP-Sen TMC: Dissociation of Pancreatic Islets (non-recovered) Protocol | 1 | null | https://www.protocols.io/view/kapp-sen-tmc-dissociation-of-pancreatic-islets-non-cxz7xp9n | Juliana Alcoforado Diniz, Dylan Baker, Jessica Garofalo, Paul Robson | TITLE: KAPP-Sen TMC: Dissociation of Pancreatic Islets (non-recovered) Protocol
AUTHORS: Juliana Alcoforado Diniz, Dylan Baker, Jessica Garofalo, Paul Robson
[DESCRIPTION]
The dispersed samples were shipped cold from PRODOLABS. Prior to scRNA-seq dispersed samples from brain dead donor’s pancreatic islets were dissoci... | ["[Abstract] The dispersed samples were shipped cold from PRODOLABS.\nPrior to scRNA-seq dispersed samples from brain dead donor’s pancreatic islets\nwere dissociated as follows.", "[Cell Dissociation with Accutase] NOTE: Before beginning cell dissociation coat all the materials (pipettes, tubes, etc.) with PIM-S001GMP... |
56,737 | Perfusion Live Microscopy Using Zeiss LSM 780 and Ibidi Perfusion Sets with SPY650 DNA Dye | 1 | null | https://www.protocols.io/view/perfusion-live-microscopy-using-zeiss-lsm-780-and-b3m9qk96 | Emir Bora Akmeriç | TITLE: Perfusion Live Microscopy Using Zeiss LSM 780 and Ibidi Perfusion Sets with SPY650 DNA Dye
AUTHORS: Emir Bora Akmeriç
[DESCRIPTION]
Step by step protocol for setting up live microscopy experiments with Ibidi perfusion sets
[STEPS]
SECTION: Cell Seeding
1. Check whether HUVECs in T25/T75 are confluent
SECT... | ["[Cell Seeding] Check whether HUVECs in T25/T75 are confluent", "[Cell Seeding] Gelatinize 2 or 3 Ibidi 0.4 luer u-slides with 0.2% gelatin in water", "[Cell Seeding] Bring trypsin, PBS, media and FBS to to 37C inside cell culture incubator", "[Cell Seeding] Trypsinize dish and count cells. A minimum of 500k cells are... |
19,134 | Sacrificing Rat | null | dx.doi.org/10.17504/protocols.io.ww6ffhe | null | Shaina Robbins, Alison Moss | TITLE: Sacrificing Rat
AUTHORS: Shaina Robbins, Alison Moss
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is the standard procedure we use when sacrificing our Sprague-Dawley rats.</div></div>
[STEPS]
?. [Sacrificing Rat]
Expose animal to 5% isoflurane by inhilation until unconsiousSacrifice... | ["[Sacrificing Rat]\nExpose animal to 5% isoflurane by inhilation until unconsiousSacrifice animal by means of decapitationHarvest organs per experimental design"] |
77,700 | In situ Pax5 detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues | 4 | dx.doi.org/10.17504/protocols.io.bp2l69pqrlqe/v1 | https://www.protocols.io/view/in-situ-pax5-detection-in-formalin-fixed-paraffin-cp5cvq2w | Jayne E Wiarda, Crystal Loving | TITLE: In situ Pax5 detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues
AUTHORS: Jayne E Wiarda, Crystal Loving
[DESCRIPTION]
An immunohistochemistry (IHC) staining protocol for in situ identification of porcine Pax5
[BEFORE_START]
Starting specimens:
Starting samples = FFPE tissues cut to 4 micron thic... | ["[Baking] Before starting the assay: \nPreheat a dry oven to 60℃ \nLoad slides for assay into vertical slide rack\n\nBaking\nBake slides 20 min 60℃\n\nWhile slides bake:\nPrepare 0.05% PBS-T (can store at RT up to 1 month)\n\nImmediately before deparaffinizing:\nAdd ~200 mL xylenes to each of three clearing agent dish... |
52,691 | A High-Throughput Assay for Quantifying Phenotypic Traits of Microalgae | 4 | dx.doi.org/10.17504/protocols.io.4r3l24j33g1y/v1 | https://www.protocols.io/view/a-high-throughput-assay-for-quantifying-phenotypic-bxptpmnn | Phoebe Argyle, Jana Hinners, Nathan G. Walworth, Sinéad Collins, Naomi M. Levine, Martina A. Doblin | TITLE: A High-Throughput Assay for Quantifying Phenotypic Traits of Microalgae
AUTHORS: Phoebe Argyle, Jana Hinners, Nathan G. Walworth, Sinéad Collins, Naomi M. Levine, Martina A. Doblin
[DESCRIPTION]
This outlines a workflow for measuring 10 phenotypic traits of centric diatoms using a variety of methodologies. This... | ["[Set up experimental cultures] Experimental cultures are grown in 12-well tissue culture plates. Triplicate cultures per treatment are recommended.\n\nThe initial cell concentration should be 2000 cells/mL, but may be altered depending on the anticipated growth. \n\nDuring developing, 400 µL of stock culture at a co... |
80,258 | RNA Extraction Protocol for Leaves with High Content of Secondary Metabolites | 4 | dx.doi.org/10.17504/protocols.io.6qpvr4n9ogmk/v1 | https://www.protocols.io/view/rna-extraction-protocol-for-leaves-with-high-conte-csmawc2e | Wesley Elias Bhering Barrios, Débora Gonçalves Gouveia | TITLE: RNA Extraction Protocol for Leaves with High Content of Secondary Metabolites
AUTHORS: Wesley Elias Bhering Barrios, Débora Gonçalves Gouveia
[DESCRIPTION]
Protocol used for extraction of total RNA from plant material rich in secondary metabolites.
[BEFORE_START]
Important details:
*Sterilize everything before... | ["[Procedure] Collect the plant material and freeze it immediately in liquid nitrogen;", "[Procedure] Macerate and transfer approximately 20 to 30 mg (max. 50 mg) to a 1.5 mL microtube;", "[Procedure] Add 500 uL of TRIzol to every 3 tubes at once (with two people, otherwise from tube to tube if alone);", "[Procedure] H... |
84,402 | DNA/RNA extraction from fresh-frozen tissue, AllPrep DNA/RNA/miRNA Universal Kit | 4 | dx.doi.org/10.17504/protocols.io.kxygx3mrwg8j/v2 | https://www.protocols.click/view/dna-rna-extraction-from-fresh-frozen-tissue-allpre-cwnsxdee | Annika Fendler | TITLE: DNA/RNA extraction from fresh-frozen tissue, AllPrep DNA/RNA/miRNA Universal Kit
AUTHORS: Annika Fendler
[DESCRIPTION]
Protocol for combined RNA and DNA extraction from fresh-frozen tissue using the AllPrep DNA/RNA/miRNA Universal Kit.
[BEFORE_START]
Preparations:
FRN buffer: Add 42 ml Isoprop to new bottle
RP... | ["[Tissue preparation] This protocol is for \nOptional: Weigh tissue\nEnter a complete list of samples used for each experiment below:", "[Tissue preparation] Transfer tissue in 350 µL 600 µL RLT + ß-ME in 2 ml DNA LoBind tube.\n\nMake sure that the tissue does not defrost.", "[Tissue preparation] Add a 5mm bead to ... |
58,786 | Preparation of Cultured Cells for Serial Block Face Scanning Electron Microscopy (SBEM) | 4 | dx.doi.org/10.17504/protocols.io.b5naq5ae | https://www.protocols.io/view/preparation-of-cultured-cells-for-serial-block-fac-b5naq5ae | Daniela Boassa, Thomas J. Deerinck, Eric A. Bushong, Andrea Thor, Mark Ellisman | TITLE: Preparation of Cultured Cells for Serial Block Face Scanning Electron Microscopy (SBEM)
AUTHORS: Daniela Boassa, Thomas J. Deerinck, Eric A. Bushong, Andrea Thor, Mark Ellisman
[DESCRIPTION]
Serial block-face scanning electron microscopy (SBEM) is a 3D EM method that allows volume reconstruction of biological ... | ["Cells are fixed in glutaraldehyde (2.5% in 0.1M sodium cacodylate) for 60 minutes (room temperature to ice).", "Cells are washed 5 x 2 minutes in cold cacodylate buffer containing 3mM calcium chloride.", "Right before use, a solution containing 3% potassium ferrocyanide in 0.2M cacodylate buffer with 6mM calcium chlo... |
37,443 | Passaging adherent cancer cell lines | 1 | dx.doi.org/10.17504/protocols.io.bgtbjwin | https://www.protocols.io/view/passaging-adherent-cancer-cell-lines-bgtbjwin | Emily Souster, Verity Goodwin, Adam Jackson, Charlotte Beaver, Fiona Behan, Rizwan Ansari, Mathew Garnett | TITLE: Passaging adherent cancer cell lines
AUTHORS: Emily Souster, Verity Goodwin, Adam Jackson, Charlotte Beaver, Fiona Behan, Rizwan Ansari, Mathew Garnett
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This SOP is for the routine maintenance and expansion of adherent cancer cell lines. </div><d... | ["[Detaching and collecting cells]\nRemove the old culture media using a sterile aspirator pipette.", "[Detaching and collecting cells]\nGently wash the cells with PBS (see Table 1, column B for recommended volume). Aspirate the PBS from the flask. ABCD1Size of flaskVolume of PBS (ml)Volume of TrypLE (ml)Volume of med... |
69,011 | DoTA-seq V3 | 4 | dx.doi.org/10.17504/protocols.io.n92ldzox7v5b/v1 | https://www.protocols.io/view/dota-seq-v3-cfmttk6n | freeman.lan | TITLE: DoTA-seq V3
AUTHORS: freeman.lan
[DESCRIPTION]
This protocol describes the process of DoTA-seq generating a single cell sequencing library from a cell suspension. This workflow can be performed in two days, with the PCR step happening overnight. Before beginning this workflow make sure to have:
1. The necessar... | ["[Preparing Cells] Prepare a cell suspension by washing twice in 1mL of by spinning down at 5000 x g, 1 min", "[Preparing Cells] Add 1 µL 10,000X dye to the cells to stain them", "[Preparing Cells] Count cells using a hemacytometer using the SYBR signal, calculate concentration of the cell suspension.", "[Prepari... |
58,413 | Striatal dopamine measurement through HPLC | 4 | dx.doi.org/10.17504/protocols.io.dm6gpbjdplzp/v1 | https://www.protocols.io/view/striatal-dopamine-measurement-through-hplc-b5amq2c6 | Pranay Srivastava, Waijiao Cai, Xiqun Chen | TITLE: Striatal dopamine measurement through HPLC
AUTHORS: Pranay Srivastava, Waijiao Cai, Xiqun Chen
[DESCRIPTION]
Protocol for striatal dopamine measurement in mouse brain
[STEPS]
1. Weigh the striatal tissue
Note - make sure to keep it frozen – can keep it on dry ice for the process
2. Add 20x PE buffer suppleme... | ["Weigh the striatal tissue \nNote - make sure to keep it frozen – can keep it on dry ice for the process", "Add 20x PE buffer supplemented with an internal standard solution to tissue. \n(eg. 10 mg sample tissue would receive 200 uL of PE solution)", "Use a Teflon pestle to homogenize the tissue into the solution by c... |
17,588 | Collection of Media and Culturing recipes for marine fungi | 2 | dx.doi.org/10.17504/protocols.io.5qpvon37zl4o/v1 | https://www.protocols.click/view/collection-of-media-and-culturing-recipes-for-mari-veue3ew | Amy Gladfelter | TITLE: Collection of Media and Culturing recipes for marine fungi
AUTHORS: Amy Gladfelter
[DESCRIPTION]
Media and culturing recipes for marine fungi
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.f5bbq2n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 12">
<div class="layoutArea">
<div class="column">The steps for preparing the lysate are different depending on the starting material. Please ensure you follow the correct procedure for your starting material (see the section <a href="https://www.pr... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.j8dcrs6 | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
<p>Use the solution of 1% carrageenan fresh (prepare right before the laboratory procedure)</p>
[GUIDELINES]
<p>To ensure a better performance, use cold PBS 1x (4C) and room temperature 1% carrageenan</p>
[STEPS]
?.
?.
?.
?. | [] |
36,026 | The Crick COVID-19 RT-PCR Testing Pipeline | null | dx.doi.org/10.17504/protocols.io.bfe2jjge | https://www.protocols.io/view/the-crick-covid-19-rt-pcr-testing-pipeline-bfe2jjge | The Crick COVID-19 Consortium | TITLE: The Crick COVID-19 RT-PCR Testing Pipeline
AUTHORS: The Crick COVID-19 Consortium
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The Crick COVID-19 Consortium commenced on 19 March 2020 in response to the emerging COVID-19 pandemic in a collaboration between University College London Hospita... | [] |
35,745 | Dephosphorylation of 5´-ends of DNA using rSAP in Restriction Enzyme Reaction (M0371) | 1 | dx.doi.org/10.17504/protocols.io.be59jg96 | https://www.protocols.io/view/dephosphorylation-of-5-ends-of-dna-using-rsap-in-r-be59jg96 | New England Biolabs | TITLE: Dephosphorylation of 5´-ends of DNA using rSAP in Restriction Enzyme Reaction (M0371)
AUTHORS: New England Biolabs
[DESCRIPTION]
This protocol explains methods for dephosphorylation of 5´-ends of DNA using rSAP in restriction enzyme reaction (M0371).
[STEPS]
1. Digest 1 µg–5 µg in a 20 μl reaction as follow... | ["Digest 1 µg–5 µg in a 20 μl reaction as follows:\n\n DNA ≥ 1 μl Restriction Enzyme Buffer (10X) 2 μl Restriction Endonuclease 1 μl H2O, purified to 20 μl**", "Incubate at 37 °C for 60 min or follow manufacturer’s recommendations.", "Add 1 unit of rSAP for every 1 pmol of DNA ends (about 1 µg of a 3 kb plasmi... |
82,745 | Western blotting | 1 | dx.doi.org/10.17504/protocols.io.ewov1oemklr2/v1 | https://www.protocols.click/view/western-blotting-cu2zwyf6 | Marine Houdou, rosanne.wouters, Peter Vangheluwe | TITLE: Western blotting
AUTHORS: Marine Houdou, rosanne.wouters, Peter Vangheluwe
[DESCRIPTION]
Protocol to detect proteins via Western Blotting.
[STEPS]
SECTION: Harvesting cells
1. collect the cells by scrapping them with a scrapper in Dulbecco’s Phosphate Buffered saline modified without calcium chloride and magne... | ["[Harvesting cells] collect the cells by scrapping them with a scrapper in Dulbecco’s Phosphate Buffered saline modified without calcium chloride and magnesium chloride (DPBS) or, using 0.25% Trypsin-EDTA for which stop enzymatic reaction by adding culture medium.", "[Harvesting cells] Centrifuge cell suspensions at ... |
null | null | null | dx.doi.org/10.17504/protocols.io.u8rezv6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describes a wet-lab method to identify Leishmania proteins bands recognized by human IgG1. The cytosolic proteins obtained by cell lysis are initially separated by tricine gel electrophoresis using a long (14 cm) apparatus (SG-200, CBS Scientific, USA). Upon comple... | ["[Protein separation by tricine SDS-PAGE] Prepare 10x solutions of cathode buffer (Tris 1M, Tricine 1M, SDS 1%, pH ~8,25) and anode buffer (Tris 1M, HCl 0.225M, pH 8.9)", "[Protein separation by tricine SDS-PAGE] Prepare an acrylamide-bisacrylamide (AB-3) stock solution by dissolving 48 g of acrylamide and 1.5g of bis... |
45,454 | Rapid Protocol for Single Sporing Ascochyta rabiei | 4 | null | https://www.protocols.io/view/rapid-protocol-for-single-sporing-ascochyta-rabiei-bqmnmu5e | Melody Christie, Kevin Moore | TITLE: Rapid Protocol for Single Sporing Ascochyta rabiei
AUTHORS: Melody Christie, Kevin Moore
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>A rapid protocol for single sporing of </span><span style = "font-style:italic;">A. rabiei.</span><span> This protocol reduces the time required for s... | ["Pour water agar plates (~ 2 cm thickness and containing antibiotic) 1-2 days before culturing to ensure plates are dry.", "Autoclave 20 ml glass bottles (McCartney) containing 10 ml milliQ water with aluminum lids loosely on. Allow to cool to room temperature.", "Add ~ 2 drops of (approximately ) to each bottle.\n30... |
44,144 | Breath Analysis System for Detecting Breath Pattern Signature of COVID-19 | 2 | dx.doi.org/10.17504/protocols.io.bpcqmivw | https://www.protocols.io/view/breath-analysis-system-for-detecting-breath-patter-bpcqmivw | Salvatore D Morgera, Tiffany Miller, Arash Takshi, Stephen E Saddow, Matthew Palm | TITLE: Breath Analysis System for Detecting Breath Pattern Signature of COVID-19
AUTHORS: Salvatore D Morgera, Tiffany Miller, Arash Takshi, Stephen E Saddow, Matthew Palm
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The magnitude of how rapidly the COVID-19 virus could spread and infect others... | [] |
66,614 | Sonovive | 3 | dx.doi.org/10.17504/protocols.io.e6nvwkyq2vmk/v1 | https://www.protocols.io/view/sonovive-cdaws2fe | Sonovive | TITLE: Sonovive
AUTHORS: Sonovive
[DESCRIPTION]
SonoVive Reviews is a viable high-level hearing well-being recipe that advances hearing well-being and supports mind capability. This supplement turns out successfully for anybody at whatever stage in life without bringing about any aftereffects.
SonoVive Customer Rev... | [] |
49,689 | Dynamic Glucose-Stimulated Insulin Secretion using Biorep Perifusion Machine (ver peri4.2) - Human or Mouse Islets | 1 | dx.doi.org/10.17504/protocols.io.3byl4kdqzvo5/v1 | https://www.protocols.click/view/dynamic-glucose-stimulated-insulin-secretion-using-burznv76 | Aliya F Spigelman, Jocelyn E Manning Fox, Patrick E Macdonald | TITLE: Dynamic Glucose-Stimulated Insulin Secretion using Biorep Perifusion Machine (ver peri4.2) - Human or Mouse Islets
AUTHORS: Aliya F Spigelman, Jocelyn E Manning Fox, Patrick E Macdonald
[DESCRIPTION]
General protocol for Dynamic Glucose-Stimulated Insulin Secretion using Biorep Perifusion Machine - Human or Mou... | ["[Day before experiment] For human islets:\n\nPick human islets of similar size and shape into Human Islet Culture Media until as close as possible to 100% purity.\n\nHuman Islet Culture Media", "[Day before experiment] For mouse islets:\n\nIsolate mouse islets as described in Mouse Islet Isolation protocol.\n\nPick t... |
53,962 | Environmental sample collection for eDNA analysis | 1 | dx.doi.org/10.17504/protocols.io.byxipxke | https://www.protocols.io/view/environmental-sample-collection-for-edna-analysis-byxipxke | Luca Mirimin, Dulaney Miller, Sara Fernandez | TITLE: Environmental sample collection for eDNA analysis
AUTHORS: Luca Mirimin, Dulaney Miller, Sara Fernandez
[DESCRIPTION]
This document describes a series of protocols for the collection of environmental samples intended for the monitoring and surveillance of marine invasive species by means of eDNA metabarcodin... | ["[Protocol for collection of water samples (high volume water)] DEPLOYMENT AND RETRIEVAL", "[Protocol for collection of water samples (high volume tow net)] SAMPLE COLLECTION", "[Protocol for collection of sediment samples] SAMPLE COLLECTION", "[Protocol for collection of water samples (low volume water)] SAMPLING", "... |
null | null | null | dx.doi.org/10.17504/protocols.io.s7aehie | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Atmospheric methane is rapidly lost when it enters humid subterranean critical and vadose zones (e.g., air in soils and caves). Because methane is a source of carbon and energy, it can be consumed by methanotrophic methane-oxidizing bacteria. As an additional subterranean sin... | ["[Apparatus at Indiana University for active, time-resolved measurements of gas concentrations with circular flow] {\"blocks\":[{\"key\":\"20mmd\",\"text\":\"We constructed an experimental apparatus to assess the loss of CH4 in an active (i.e., with pumping of air) and time-resolved manner with or without added radiat... |
91,775 | DToL Specimen Tissue, Blood and Voucher Sampling Standard Operating Procedure: Chordata: Vertebrata: Fish | 1 | dx.doi.org/10.17504/protocols.io.14egn3y6pl5d/v1 | https://www.protocols.io/view/dtol-specimen-tissue-blood-and-voucher-sampling-st-c5u7y6zn | Inez Januszczak, James Maclaine | TITLE: DToL Specimen Tissue, Blood and Voucher Sampling Standard Operating Procedure: Chordata: Vertebrata: Fish
AUTHORS: Inez Januszczak, James Maclaine
[DESCRIPTION]
This Standard Operating Procedure (SOP) covers how fish specimens are to be sampled and preserved for vouchering at the Natural History Museum (NHM) i... | [] |
66,351 | Bridport Health Liver Support Healthy Liver Cells Lose And Liver Fat Where To Buy Advantages Price(Work Or Hoax) | 3 | dx.doi.org/10.17504/protocols.io.14egn7q7yv5d/v1 | https://www.protocols.io/view/bridport-health-liver-support-healthy-liver-cells-cc2psydn | Bridport Health Liver Support | TITLE: Bridport Health Liver Support Healthy Liver Cells Lose And Liver Fat Where To Buy Advantages Price(Work Or Hoax)
AUTHORS: Bridport Health Liver Support
[DESCRIPTION]
CLICK HERE TO BUY Bridport Health “GET 50% DISCOUNT” NO.1 MALE ENHANCEMENT 2022 HURRY LIMITED TIME OFFER!!
[STEPS] | [] |
10,817 | plasma preparation_test | 1 | dx.doi.org/10.17504/protocols.io.ns9deh6 | https://www.protocols.io/view/plasma-preparation-test-ns9deh6 | Anneleen Decock | TITLE: plasma preparation_test
AUTHORS: Anneleen Decock
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to prepare plasma from blood and is an experimental protocol.</div></div>
[STEPS]
?. [Blood draw]
Note blood tube typeNote date and time point of blood collection; sto... | ["[Blood draw]\nNote blood tube typeNote date and time point of blood collection; store tubes upright at room temperature until centrifugationNote date and time point of arrival in labNote amount of blood that was collectedTake pictures of the tubes if needed", "[Centrifugation step 1: 20 min at 400 g (rcf)]\nInvert tu... |
58,829 | Coffee Protocol | 1 | null | https://www.protocols.io/view/coffee-protocol-b5pmq5k6 | John Borghi | TITLE: Coffee Protocol
AUTHORS: John Borghi
[DESCRIPTION]
This protocol is intended as a demonstration of what I like to call the Coffee Cup Rule - Document more than you think is necessary and with greater specificity that you think is necessary.
The name is derived from the fact that the cup measures on drip coff... | ["[Make Coffee] Measure out 1000 g and 60 g. \n\nIf the scale is out of batteries, 4 1/4 cups (US) of water and 8 tablespoons of coffee will work in a pinch. \n\nUse a measuring cup for the water not the cup measures on the side of the coffee pot. Those refer to \"cups of coffee\".", "[Make Coffee] Pour the water in t... |
85,338 | Analysis of sodium monofluoracetate (compound 1080) in animal kidney tissue by LC-MS/MS | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdk57lmk/v1 | https://www.protocols.io/view/analysis-of-sodium-monofluoracetate-compound-1080-cxj2xkqe | Jim Langston | TITLE: Analysis of sodium monofluoracetate (compound 1080) in animal kidney tissue by LC-MS/MS
AUTHORS: Jim Langston
[DESCRIPTION]
This diagnostic method provides for the identification and quantitative analysis of sodium monofluoroacetate (Compound 1080) from kidney tissue using LC-MS/MS
Sodium monofluoroacetate (Co... | ["[Prepare Reagents] 5% Ammonium hydroxide in water", "[Prepare Reagents] Combine 10 mL of ammonium hydroxide with 190 mL of water'", "[Prepare Reagents] 0.5% Trifluoroacetic acid in acetonitrile", "[Prepare Reagents] Combine5 mL of trifluoroacetic acid with 995 mL of water", "[Prepare Reagents] 5 mM Ammonium formate, ... |
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