id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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83,214 | DNA extraction (BOMB) | 4 | dx.doi.org/10.17504/protocols.io.n2bvj6mdnlk5/v6 | https://www.protocols.click/view/dna-extraction-bomb-cvhnw35e | Yin-Tse Huang, Tsu-Chun Hung | TITLE: DNA extraction (BOMB)
AUTHORS: Yin-Tse Huang, Tsu-Chun Hung
[DESCRIPTION]
DNA extraction (BOMB)
[STEPS]
SECTION: Sample Collection
1. Add 200 µL of 0.5 mm beads to 2mL screw tube
SECTION: Sample Collection
2. Add200 µL of 1 mm beads to 2mL screw tube
SECTION: Sample Collection
3. Add870 µL Lysis master m... | ["[Sample Collection] Add 200 µL of 0.5 mm beads to 2mL screw tube", "[Sample Collection] Add200 µL of 1 mm beads to 2mL screw tube", "[Sample Collection] Add870 µL Lysis master mix to 2mL screw tube. The final look:", "[Sample Collection] Collect 20-50 mg of sample to 2mL screw tube", "[Sample crush] Put the 2mL screw... |
94,621 | Sinai SCENT TMC - Peripheral Blood Collection | 4 | dx.doi.org/10.17504/protocols.io.ewov1q6q7gr2/v1 | https://www.protocols.io/view/sinai-scent-tmc-peripheral-blood-collection-c8m5zu86 | Santos Bermejo, Sojin Kim, Patty J Lee | TITLE: Sinai SCENT TMC - Peripheral Blood Collection
AUTHORS: Santos Bermejo, Sojin Kim, Patty J Lee
[DESCRIPTION]
This Standard Operating Procedure is to provide guidance toresearch team members who are involved in the retrieval, processing, and storing of peripheral blood samples obtained from research participants.... | ["[Scope] This document outlines the process of collecting, processing, and storing peripheral blood from research participants enrolled in SCENT U54.", "[Materials] Blood Collection \n2x Yellow Top Tubes (PBMC) - Acid citric dextrose additive (ACD) \n1x Red Top Tube (Serum) – No additive \n3x Purple Top (Plasma) - Eth... |
13,781 | AFLP RedTaq protocol | 1 | dx.doi.org/10.17504/protocols.io.rpvd5n6 | https://www.protocols.io/view/aflp-redtaq-protocol-rpvd5n6 | Michal Ronikier, Tomasz Suchan | TITLE: AFLP RedTaq protocol
AUTHORS: Michal Ronikier, Tomasz Suchan
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Restriction-ligation reaction]
[water]
[T4 DNA Ligase buffer (10×)]
[BSA (1 mg/ml)]
[NaCl (0.5 M)]
[Adapter MseI (50 µM)]
[Adapter EcoRI (5 µM)]
[MseI (10 U/µl)]
[EcoRI (20 U/µl)]
[T4 DNA Li... | ["[Restriction-ligation reaction]\n[water]\n[T4 DNA Ligase buffer (10×)]\n[BSA (1 mg/ml)]\n[NaCl (0.5 M)]\n[Adapter MseI (50 µM)]\n[Adapter EcoRI (5 µM)]\n[MseI (10 U/µl)]\n[EcoRI (20 U/µl)]\n[T4 DNA Ligase (5U/µl)]", "[Restriction-ligation reaction]\nIncubate at 37ºC for 3 h and at 17 °C overnight.", "[Restriction-lig... |
25,882 | Ex vivo differentiation of resting CD4+ T cells coupled with the QVOA (dQVOA) | null | dx.doi.org/10.17504/protocols.io.5h2g38e | null | Elizabeth Wonderlich, Krupa Subramanian, Carol Lackman-Smith , Roger Ptak, Deanna Kulpa | TITLE: Ex vivo differentiation of resting CD4+ T cells coupled with the QVOA (dQVOA)
AUTHORS: Elizabeth Wonderlich, Krupa Subramanian, Carol Lackman-Smith , Roger Ptak, Deanna Kulpa
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Day -8]
Thaw and prepare cryopreserved PBMC from cohort or clinical trial pa... | ["[Day -8]\nThaw and prepare cryopreserved PBMC from cohort or clinical trial participants.Typically, 50-100x106 cryopreserved PBMC are used to yield sufficient resting CD4+ T cells for each dQVOA. For the referenced publication, a typical dQVOA used 8x106 rCD4+ T cells.", "[Day -7]\nPerform resting enrichment of CD4+ ... |
62,964 | Tranquileafz CBD Gummies Canada are not open to being bought in any shop or online store? | 3 | dx.doi.org/10.17504/protocols.io.5qpvob8k7l4o/v1 | https://www.protocols.io/view/tranquileafz-cbd-gummies-canada-are-not-open-to-be-b9qur5ww | TranquileafzCBDBUY | TITLE: Tranquileafz CBD Gummies Canada are not open to being bought in any shop or online store?
AUTHORS: TranquileafzCBDBUY
[DESCRIPTION]
Specialists profoundly acclaim this sticky and individuals have proactively begun to feel the mending they had been searching for.
[STEPS] | [] |
85,176 | unc-80 LoF Mutant Drug Repurposing/Confirmation Screening | 4 | dx.doi.org/10.17504/protocols.io.5jyl8p5yrg2w/v1 | https://www.protocols.click/view/unc-80-lof-mutant-drug-repurposing-confirmation-sc-cxeyxjfw | Thomas J O'Brien | TITLE: unc-80 LoF Mutant Drug Repurposing/Confirmation Screening
AUTHORS: Thomas J O'Brien
[DESCRIPTION]
Combined protocol for conducting a drug repurposing screen of the MRCT FDA-approved compound library (743 compounds) to find drugs that rescue the behavioural phenotype of adult unc-80(syb1531) C. elegans mutants, ... | ["[Pick L4 worms for bleaching (9 days prior to tracking)] Pick 10 x L4 unc-80(syb1531) worms onto 20 x 90mm NGM-agar plates, and 10 x L4 N2 (wild-type) worms onto 2 x 90mm NGM-agar plates pre-seeded with E. coli OP50 and incubate at 20°C.\n(22 plates will be picked in total)\n\nIn this screen we are looking for compou... |
44,074 | Impact of Post COVID Physiotherapy and Rehabilitation in Bangladesh | 1 | dx.doi.org/10.17504/protocols.io.bpaimice | https://www.protocols.io/view/impact-of-post-covid-physiotherapy-and-rehabilitat-bpaimice | anwar_physiobd , Dr Iqbal Kabir Jahid, K M Amran Hossain, Physiotherapist Shahoriar Ahmed , shafin.rubayet00 | TITLE: Impact of Post COVID Physiotherapy and Rehabilitation in Bangladesh
AUTHORS: anwar_physiobd , Dr Iqbal Kabir Jahid, K M Amran Hossain, Physiotherapist Shahoriar Ahmed , shafin.rubayet00
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"><span ... | ["BaselineFrom All 2000 participants will be laboratory-confirmed post-covid 19 recovered participants, 500 participants will be selected via simple random sampling procedure by computerized random conceal allocation", "Baseline assessment act as pretest data", "Group A 165±10", "Group A will follow the booklet instruc... |
57,787 | Genotyping by next generation sequencing | 2 | dx.doi.org/10.17504/protocols.io.b4n3qvgn | https://www.protocols.io/view/genotyping-by-next-generation-sequencing-b4n3qvgn | Hanqin Li, Yogendra Verma, Dirk Hockemeyer, Frank Soldner | TITLE: Genotyping by next generation sequencing
AUTHORS: Hanqin Li, Yogendra Verma, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This collection describes a standard genotyping procedure using next generation sequencing (NGS) in the Hockemeyer lab.
Collection overview
Preparing of genomic DNA from in vitro cultured ... | [] |
45,201 | three hours sleep restriction protocol | 1 | null | https://www.protocols.io/view/three-hours-sleep-restriction-protocol-bqdrms56 | longzhiliang | TITLE: three hours sleep restriction protocol
AUTHORS: longzhiliang
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol was obtained from the Sleepy Brain Project (</span><a href="https://openneuro.org/datasets/ds000201" style = "text-decoration:underline;color:blue;cursor:pointer;"... | ["Participants were recruited by poster and newspaper advertising based on the following inclusion coriteria: 1) those required to undergo fMRI procedures, e.g., no ferromagnetic items in the body, not claustrophobic, and not pregnant; 2) those who have no current or past self-reported psychiatric or neurological illne... |
99,474 | 2D differentiation of NPCs to neurons & astrocytes | 0 | null | https://www.protocols.io/view/2d-differentiation-of-npcs-to-neurons-amp-astrocyt-ddds226e | Jessie Buth | TITLE: 2D differentiation of NPCs to neurons & astrocytes
AUTHORS: Jessie Buth
[DESCRIPTION]
Protocol to differentiate neural progenitor cells to mature neurons and astrocytes in 2D culture.
[STEPS]
SECTION: Thawing Neural Progenitor Cells (NPCs)
3. See protocol for thawing hematopoietic progenitor cells (HPCs)... | ["[Thawing Neural Progenitor Cells (NPCs)] See protocol for thawing hematopoietic progenitor cells (HPCs).\n https://www.protocols.io/view/thawing-frozen-hematopoietic-stem-cells-hpcs-dddt226n\n \nIt is the same procedure for NPCs, except:\nthe plates are coated with 0.5 mg/mL standard matrigel\nthe cells are maint... |
94,867 | Congo Red Immunostaining | 4 | null | https://www.protocols.io/view/congo-red-immunostaining-c8vtzw6n | daniel.dautan, Per Svenningsson | TITLE: Congo Red Immunostaining
AUTHORS: daniel.dautan, Per Svenningsson
[DESCRIPTION]
Staining for amyloid structures in protein aggregates
[STEPS]
1. Wash freshly sectioned tissues 2-3 times with 1X PBS to remove OCT.
2. Mount all sections onto microscope slides and dry at Room temperature .
3. Transfer slides i... | ["Wash freshly sectioned tissues 2-3 times with 1X PBS to remove OCT.", "Mount all sections onto microscope slides and dry at Room temperature .", "Transfer slides into a 1% CongoRed solution for 30 min.", "Place slides into alkaline bath (1% Sodium hydroxide in 50% ethanol) for 5 min.", "Transfer slides into 3 sequen... |
29,647 | PBMC isolation and cryopreservation | null | dx.doi.org/10.17504/protocols.io.87phzmn | null | Marloes van Splunter | TITLE: PBMC isolation and cryopreservation
AUTHORS: Marloes van Splunter
[STEPS] | [] |
43,669 | BSCI:414 Lab 8 Transform and Miniprep Plasmid Containing SARS-CoV-2 Spike Gene | 1 | null | https://www.protocols.io/view/bsci-414-lab-8-transform-and-miniprep-plasmid-cont-bnvvme66 | Harley King | TITLE: BSCI:414 Lab 8 Transform and Miniprep Plasmid Containing SARS-CoV-2 Spike Gene
AUTHORS: Harley King
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Received 3ul plasmid containing SARS-CoV-2 spike gene from Xiaopin Zhu at UMD. I performed transformation and plated on LB plates containing 100u... | ["Watch miniprep video.", "Transform \"pHDM-Spike\" plasmid containing SARS-CoV-2 spike gene into chemically competent Dh10B cells and select on LB ampicillin plates.", "Receive plasmid containing SARS-CoV-2 spike gene from Xiaoping Zhu @ UMD VetMed.Link to plasmid in Benchling.", "Quantify plasmid concentration using ... |
98,316 | Beer Choice in Moorea | 0 | dx.doi.org/10.17504/protocols.io.5qpvok5d7l4o/v1 | https://www.protocols.io/view/beer-choice-in-moorea-db9k2r4w | Alexander Ferrera | TITLE: Beer Choice in Moorea
AUTHORS: Alexander Ferrera
[DESCRIPTION]
It seems like everywhere you go in Moorea someone is always holding a beer, and that beer is always Hinano Tahiti. Hinanos come either in small six-pack of cans , four-tall boys packs of can, and as bottles in a crate that holds twenty. The beer cr... | ["[Survey] Create a survey in French. the questions will ask how much beer the participant drinks and which beer packaging (cans or bottles) the participant prefers and why.", "[Survey] Hand out the survey digitally or physically to around 500-1000 Moorea residents through local connections (Virihei).", "[Survey] From ... |
null | null | null | dx.doi.org/10.17504/protocols.io.p7zdrp6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
91,707 | Human Brain Sequential Extraction (Tau) | 1 | dx.doi.org/10.17504/protocols.io.q26g7p9y8gwz/v1 | https://www.protocols.io/view/human-brain-sequential-extraction-tau-c5s3y6gn | Michael X. Henderson | TITLE: Human Brain Sequential Extraction (Tau)
AUTHORS: Michael X. Henderson
[DESCRIPTION]
This protocol details Human Brain Sequential Extraction (Tau). This protocol is an adaptation of the work of several labs.
[STEPS]
SECTION: 1 Day Before Extraction
1.
SECTION: 1 Day Before Extraction
2. Make sure glass 100 ... | ["[1 Day Before Extraction]", "[1 Day Before Extraction] Make sure glass 100 or 40 mL homogenizers and pestles (A and B) are cleaned with 70%\nethanol, wrapped in foil, and autoclaved.", "[1 Day Before Extraction] Clean out ultracentrifuge tubes and caps with 70% ethanol and dry.", "[1 Day Before Extraction] Transfer b... |
47,183 | Overview of NCBI's submission process and the metadata required | 1 | dx.doi.org/10.17504/protocols.io.bsbpnamn | https://www.protocols.io/view/overview-of-ncbi-39-s-submission-process-and-the-m-bsbpnamn | Ruth Timme, Emma Griffiths, Lee Katz | TITLE: Overview of NCBI's submission process and the metadata required
AUTHORS: Ruth Timme, Emma Griffiths, Lee Katz
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">PURPOSE: </span></div><div class = "text-block"><span style = "font-weight:bold;">This protocol e... | ["[Three templates needed for NCBI SARS-CoV-2 submission]\nSTART HERE FIRST: Read the PHA4GE contextual data specification BEFORE populating your submission templates!", "[BioSample metadata]\nPHA4GE pathogen template for BioSample submission:Download File:Follow guidance presented in this file for populating the temp... |
65,258 | Myco Nootropic Brain Gummies Review – Is MycoMode Smart Gummy Scam or Legit? | 3 | dx.doi.org/10.17504/protocols.io.6qpvr6y8ovmk/v1 | https://www.protocols.io/view/myco-nootropic-brain-gummies-review-is-mycomode-sm-cbyispue | darnellbianco | TITLE: Myco Nootropic Brain Gummies Review – Is MycoMode Smart Gummy Scam or Legit?
AUTHORS: darnellbianco
[DESCRIPTION]
MycoMode Nootropic Brain Gummies
[STEPS] | [] |
45,951 | Frozen stocks | 4 | null | https://www.protocols.io/view/frozen-stocks-bq47myzn | Elizabeth Fozo | TITLE: Frozen stocks
AUTHORS: Elizabeth Fozo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Preparation of frozen stocks for the PERMANENT lab collection</div></div>
[STEPS]
?. [Steps]
Begin a 10 mL overnight of the strain in an appropriate media with antibiotics as necessary. Incubate at the appr... | ["[Steps]\nBegin a 10 mL overnight of the strain in an appropriate media with antibiotics as necessary. Incubate at the appropriate temperature overnight.", "[Steps]\nIn the morning harvest the culture by spinning cells down at 3500 RMP for 10 minutes (program 1).", "[Steps]\nResuspend the pellet in 1 mL of the media u... |
68,888 | Fluorescence recovery after photobleaching (FRAP) | 1 | dx.doi.org/10.17504/protocols.io.5qpvobj29l4o/v1 | https://www.protocols.io/view/fluorescence-recovery-after-photobleaching-frap-cfhytj7w | Xinbo Wang, Pietro De Camilli | TITLE: Fluorescence recovery after photobleaching (FRAP)
AUTHORS: Xinbo Wang, Pietro De Camilli
[DESCRIPTION]
This protocol details methods of the FRAP analysis of LRRK2-induced liposome tubules in vitro
[STEPS]
SECTION: Fluorescence recovery after photobleaching (FRAP)
1. Prepare LRRK2-liposome mixtures in a PCR tub... | ["[Fluorescence recovery after photobleaching (FRAP)] Prepare LRRK2-liposome mixtures in a PCR tube with 300 nanomolar (nM) GFP-LRKK2, 20 micromolar (µM) liposomes (labeled with trace amounts of rhodamine-PE) and 1 millimolar (mM) GMPPNP.", "[Fluorescence recovery after photobleaching (FRAP)] Immediately deposit6 µL -1... |
null | null | null | dx.doi.org/10.17504/protocols.io.memc3c6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>this experiment describes a process to deveop a classifier which can be used to modulate the stiffness of prosthetic leg based on the movement of hands, elbows and position of the other leg of the amputee</p>
[GUIDELINES]
<p>the initial experiments shoud be conducted on heal... | [] |
55,361 | My shiny new protocol | 4 | dx.doi.org/10.17504/protocols.io.dm6gpber1lzp/v1 | https://www.protocols.io/view/my-shiny-new-protocol-b2a9qah6 | Emma Ganley | TITLE: My shiny new protocol
AUTHORS: Emma Ganley
[DESCRIPTION]
test abstract
[STEPS]
SECTION: Section 1
1.
step 1
SECTION: Section 1
2. add 50 mL and 100 mL
SECTION: section 2
4. 2 min
tesating
SECTION: Section 1
3.
SECTION: section 2
5. a new step
SECTION: Section 3
6. | ["[Section 1] step 1", "[Section 1] add 50 mL and 100 mL", "[section 2] 2 min \n\ntesating", "[Section 1]", "[section 2] a new step", "[Section 3]"] |
98,224 | FibPho: Analysis Protocol | 0 | dx.doi.org/10.17504/protocols.io.bp2l62nrzgqe/v1 | https://www.protocols.io/view/fibpho-analysis-protocol-db6q2rdw | Sasha Burwell | TITLE: FibPho: Analysis Protocol
AUTHORS: Sasha Burwell
[DESCRIPTION]
This protocol details the analysis of the fiber photometry data.
[STEPS]
SECTION: Analysis Protocol
1. Each animal has one folder per recording session generated by Synapse with all the fiber photometry recording data from that session. Make a cop... | ["[Analysis Protocol] Each animal has one folder per recording session generated by Synapse with all the fiber photometry recording data from that session. Make a copy of the \n \n generated by the behavior code from that same session and add it to the folder.", "[Analysis Protocol] To analyze the fiber photometry sign... |
91,582 | NGGDPP Collection Metadata Submission Guide | 5 | dx.doi.org/10.17504/protocols.io.6qpvr321bvmk/v1 | https://www.protocols.io/view/nggdpp-collection-metadata-submission-guide-c5n6y5he | darthur | TITLE: NGGDPP Collection Metadata Submission Guide
AUTHORS: darthur
[DESCRIPTION]
The Registry of Scientific Collections (ReSciColl) is a metadata catalog administered by the National Geological and Geophysical Data Preservation Program (NGGDPP), It includes essential metadata describing scientific collections, to pro... | ["[Contacts - Importing] In mdEditor, Contacts are created and maintained as separate records from metadata records. This enables a contact to be used many times in a single metadata record or across multiple metadata records without requiring a user to reenter the contact's information. Rather than entering a contact'... |
84,542 | Coastal Environmental DNA Sampling & Gravity Filtration Protocol | 1 | dx.doi.org/10.17504/protocols.io.bp2l69y7klqe/v2 | https://www.protocols.click/view/coastal-environmental-dna-sampling-amp-gravity-fil-cws6xehe | Meghan M. Shea, Alexandria B Boehm | TITLE: Coastal Environmental DNA Sampling & Gravity Filtration Protocol
AUTHORS: Meghan M. Shea, Alexandria B Boehm
[DESCRIPTION]
This is a protocol for collecting coastal environmental DNA (eDNA) samples and gravity filtering them on site, using a set-up with single-use enteral feeding pouches first described in ... | ["[Pre-Sampling Preparation] Sterilize luer lock to hose barb adapters, male-female luer lock caps, and silicone rubber cap", "[Pre-Sampling Preparation] Clean 1000 mL Nalgene bottles (as many as field blanks needed) with a 10% bleach rinse (leave for 10 minutes), then three rinses of DI water", "[Pre-Sampling Preparat... |
15,879 | Targeted PCR-based deep sequencing of cfDNA with unique molecular indices by a customized QIAseq Targeted DNA Panel | null | dx.doi.org/10.17504/protocols.io.trfem3n | null | Corinna Keup, Peter Hahn, Siegfried Hauch, Markus Sprenger-Haussels, Mitra Tewes, Pawel Mach, Ann-Kathrin Bittner, Rainer Kimmig, Sabine Kasimir-Bauer, Karim Benyaa | TITLE: Targeted PCR-based deep sequencing of cfDNA with unique molecular indices by a customized QIAseq Targeted DNA Panel
AUTHORS: Corinna Keup, Peter Hahn, Siegfried Hauch, Markus Sprenger-Haussels, Mitra Tewes, Pawel Mach, Ann-Kathrin Bittner, Rainer Kimmig, Sabine Kasimir-Bauer, Karim Benyaa
[DESCRIPTION]
<div cla... | ["[Starting material]\ncfDNA was isolated from preferably 4 ml plasma by affinity-based binding to magnetic beads according to the manufacturer’s instructions (QIAamp MinElute ccfDNA Kit; QIAGEN GmbH, Hilden, Germany). cfDNA was eluted in 22 µl ultraclean water and stored at -20°C.cfDNA concentrations were analyzed by ... |
null | null | null | dx.doi.org/10.17504/protocols.io.if3cbqn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Infected beetles were collected and homogenized in sterile water. The homogenates were filtered through four layers of cheesecloth and centrifuged at 3000g for 15 min. The pellets were resuspended in sterile water, and the spores were purified by Percoll gradient centrifugati... | [] |
28,739 | Western Analysis used in Oxidative Stress Protocols | null | dx.doi.org/10.17504/protocols.io.8bbhsin | null | Eva Feldman | TITLE: Western Analysis used in Oxidative Stress Protocols
AUTHORS: Eva Feldman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This is the general protocol used for western analysis of samples from the Oxidative Stres... | ["Wash & dry plates.", "Assemble rig and fill plates with H²O to check for leaks.", "Pour off water and wipe dry with kimwipe.", "Load gel to about the top of the door.", "Add 2-propanol to cover the edge.", "Wait ~ 40 minutes to polymerize.", "Thaw samples on ice.", "When gel is ready, pour off 2-propanol and rinse wi... |
59,888 | MSD Gold Streptavidin Antibody Preparation and Plate Run Protocol | 4 | dx.doi.org/10.17504/protocols.io.5qpvobkj9l4o/v1 | https://www.protocols.io/view/msd-gold-streptavidin-antibody-preparation-and-pla-b6qqrdvw | Kamaljot Gill, Maria Sckaff, Claire D Clelland | TITLE: MSD Gold Streptavidin Antibody Preparation and Plate Run Protocol
AUTHORS: Kamaljot Gill, Maria Sckaff, Claire D Clelland
[DESCRIPTION]
This protocol describes how to conjugate antibodies and run the Meso Scale Discovery (MSD) Sandwich enzyme-linked immunosorbent assay (ELISA) on MSD GOLD 96-well Small Spot St... | ["[Buffer Exchange the Antibodies] Chill PBS (or MSD Conjugation Buffer) and ultrapure water on ice.", "[Buffer Exchange the Antibodies] Equilibrate Zeba Spin Desalting Columns, MSD Storage Buffer, and Sulfo-NHS-LC-Biotin at Room temperature.", "[Buffer Exchange the Antibodies] Use one Zeba column per 70 µL of antibody... |
54,505 | Differentiation of hPSCs to hypothalamic neurons | 1 | dx.doi.org/10.17504/protocols.io.bzghp3t6 | https://www.protocols.io/view/differentiation-of-hpscs-to-hypothalamic-neurons-bzghp3t6 | Cortina Chen, Iman Mali, Florian T Merkle | TITLE: Differentiation of hPSCs to hypothalamic neurons
AUTHORS: Cortina Chen, Iman Mali, Florian T Merkle
[DESCRIPTION]
This protocol is about Differentiation of hPSCs to hypothalamic neurons.
[BEFORE_START]
Prepare Media and Reagents as described in section 'Materials'.
[STEPS]
SECTION: Thawing of human pluripote... | ["[Thawing of human pluripotent stem cell (hPSC) lines:] Thaw an aliquot of 1:10 diluted Geltrex on ice or in the fridge.", "[Thawing of human pluripotent stem cell (hPSC) lines:] Dilute aliquot 1:10 in ice-cold DMEM/F12 to a final concentration of 1:100.", "[Thawing of human pluripotent stem cell (hPSC) lines:] To coa... |
98,630 | Autoimmunity and The Role of T Cells in Parkinson’s Disease (PPMI Whole Blood T cells) | 0 | dx.doi.org/10.17504/protocols.io.261ge543jg47/v1 | https://www.protocols.io/view/autoimmunity-and-the-role-of-t-cells-in-parkinson-dcje2uje | David Sulzer, Cecilia Arlehamn | TITLE: Autoimmunity and The Role of T Cells in Parkinson’s Disease (PPMI Whole Blood T cells)
AUTHORS: David Sulzer, Cecilia Arlehamn
[DESCRIPTION]
This protocol details autoimmunity and the role of T cells in Parkinson’s Disease (PPMI Whole Blood T cells).
[GUIDELINES]
Appendix 1-Schedule of Activities
PPMI Whole ... | ["[PURPOSE OF STUDY] The purpose of this protocol is to study T cell reactivity to a-synuclein in prodromal and early Parkinson’s Disease (PD) participants compared to Healthy Controls (HC).\n\nAutoimmunity plays a crucial role in PD through T cell responses toward ∝-syn 1,2,8.", "[PURPOSE OF STUDY] Analysis of longitu... |
73,174 | Registro y entrada de material bibliográfico Biblioteca de la UCAM | 1 | dx.doi.org/10.17504/protocols.io.rm7vzbjm4vx1/v1 | https://www.protocols.io/view/registro-y-entrada-de-material-bibliogr-fico-bibli-cjpwumpe | Antonio Rex Alegria | TITLE: Registro y entrada de material bibliográfico Biblioteca de la UCAM
AUTHORS: Antonio Rex Alegria
[DESCRIPTION]
Registro y entrada de material bibliográfico Biblioteca de la UCAM
[STEPS]
1. Abrimos nuestra página de Excel donde introducimos los datos correspondientes al ejemplar que registramos en la Biblioteca
... | ["Abrimos nuestra página de Excel donde introducimos los datos correspondientes al ejemplar que registramos en la Biblioteca\n\n \n Nº registro Fecha Reg. Autor Título C.D.U.", "En la portada del material, ponemos el sello de la Biblioteca y escribimos el número correspondiente de la hoja de Exc... |
null | null | null | dx.doi.org/10.17504/protocols.io.euybexw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
60,026 | Determination of microglucosuria | 6 | null | https://www.protocols.io/view/determination-of-microglucosuria-b6u2reye | El Hadji Malick Ndour, Khuthala Mnika, Fatou Gueye Tall, Moussa Seck, Indou Deme Ly, Victoria Nembaware, Gaston Kuzamunu Mazandu, Helene Ange Therese Sagna-Bassene, Rokhaya Dione, Aliou Abdoulaye Ndongo, Jean Pascal Demba Diop, Nene Oumou Kesso Barry, Moustapha Djite, Rokhaya Ndiaye Diallo, Papa Madieye Gueye, Saliou ... | TITLE: Determination of microglucosuria
AUTHORS: El Hadji Malick Ndour, Khuthala Mnika, Fatou Gueye Tall, Moussa Seck, Indou Deme Ly, Victoria Nembaware, Gaston Kuzamunu Mazandu, Helene Ange Therese Sagna-Bassene, Rokhaya Dione, Aliou Abdoulaye Ndongo, Jean Pascal Demba Diop, Nene Oumou Kesso Barry, Moustapha Djite, R... | ["[DETERMINATION OF MICROGLUCOSURIA] . OBJECTIVE\nThe aim is to describe how microglucosuria is determined.", "[DETERMINATION OF MICROGLUCOSURIA] SAMPLING\n\nUrine \nA meadstream voiding urine sample at random at any time between 8 a.m. and 2 p.m. is collected. The urine sample is centrifuged before performing the tes... |
106,712 | Efficient and precise targeting of the AAVS1 safe harbour locus in hPSCs. | 0 | dx.doi.org/10.17504/protocols.io.14egn6r1ml5d/v2 | https://www.protocols.io/view/efficient-and-precise-targeting-of-the-aavs1-safe-dkfy4tpw | Dmitry Ovchinnikov | TITLE: Efficient and precise targeting of the AAVS1 safe harbour locus in hPSCs.
AUTHORS: Dmitry Ovchinnikov
[DESCRIPTION]
Stably genetically-modified human pluripotent stem cells (hPSCs) are increasingly being used for studies relying on the consistent expression of the transgene of interest in human stem cells and t... | ["[Transfection for gene targeting] 1. Prepare a desired number of wells in a 6-well plate to accommodate the hPSC cell suspension after electroporation, and become \"master\" wells for establishing targeted clones after antibiotic selection. The wells are coated with or similar ECM with 2x higher concentration relati... |
null | null | null | dx.doi.org/10.17504/protocols.io.jiqckdw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
40,227 | Peptide fragment (579-601 from HIV-gp41) conjugated to keyhole limpet haemocyanin to be used as HIV immunogen. | 6 | dx.doi.org/10.17504/protocols.io.bjibkkan | https://www.protocols.io/view/peptide-fragment-579-601-from-hiv-gp41-conjugated-bjibkkan | Angel Justiz-Vaillant | TITLE: Peptide fragment (579-601 from HIV-gp41) conjugated to keyhole limpet haemocyanin to be used as HIV immunogen.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Chemical synthesis facilitates the generation of peptides which are exceedingly difficult to express i... | ["These peptide fragment (579-601 from HIV-gp41) is dimerized by cysteine oxidation with dimethyl-sulfoxide. The HIV peptide is dissolved in 5% acetic acid to a final concentration of 5.1 mg/ml.", "The pH of the medium is adjusted to 6 with 1 M (NH4)2CO3.", "Dimethyl-sulfoxide is added to 20% of the final volume, and a... |
null | null | null | dx.doi.org/10.17504/protocols.io.h5db826 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Surface protein labeling followed by immunoprecipitation</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
61,932 | PNA-functional Ligand Conjugates Synthesis | 6 | dx.doi.org/10.17504/protocols.io.kxygxz5oov8j/v1 | https://www.protocols.io/view/pna-functional-ligand-conjugates-synthesis-b8qkrvuw | Cathy Miller | TITLE: PNA-functional Ligand Conjugates Synthesis
AUTHORS: Cathy Miller
[DESCRIPTION]
In recent years, the development of PNA into genetic drugs has been one of the research hotspots. However, the biggest obstacle leading to the poor drug ability of PNA is still the problem of cell transport. Covalently linking PNA w... | [] |
55,350 | Incubation chamber settings | 4 | null | https://www.protocols.io/view/incubation-chamber-settings-b2awqafe | Wolfram Moebius | TITLE: Incubation chamber settings
AUTHORS: Wolfram Moebius
[DESCRIPTION]
Incubation chamber settings
[STEPS]
SECTION: Chamber Settings For 30°C
1.
Newer chamber:
Lid = 32 °C
Plate = 29 °C
Glass = 28 °C
Probe reading 30 °Cthroughout
Thermal camera image 2777
Older chamber:
Lid = 33.5 °C
Plate = 31 °C
Glass = 30 °C... | ["[Chamber Settings For 30°C] Newer chamber:\nLid = 32 °C\nPlate = 29 °C\nGlass = 28 °C\nProbe reading 30 °Cthroughout\nThermal camera image 2777\n\n\nOlder chamber:\nLid = 33.5 °C\nPlate = 31 °C\nGlass = 30 °C \nProbe reading 30 °Chroughout\nThermal camera image 2786\n\nNew chamber at 30 °C\n \nOld chamber at 30 °C",... |
null | null | null | dx.doi.org/10.17504/protocols.io.ebhbaj6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This collection is part of the VERVE holiday drive to collect exotic off-the-shelf laboratory recipes. More details <a href="https://www.protocols.io/g/verve-net/news/call-for-recipes" target="_blank">here</a>.<br /><br />If you know creative/unusual protocols like these, please... | [] |
61,576 | Metagenomic extraction of high molecular weight plankton from filters | 1 | dx.doi.org/10.17504/protocols.io.5jyl89by8v2w/v1 | https://www.protocols.io/view/metagenomic-extraction-of-high-molecular-weight-pl-b8dgrs3w | Benoît Vacherie, Karine Labadie | TITLE: Metagenomic extraction of high molecular weight plankton from filters
AUTHORS: Benoît Vacherie, Karine Labadie
[DESCRIPTION]
Filtration and extraction protocol of plankton metagenomic samples to obtain high molecular weight DNA for sequencing on Minion nanopore.
The protocol describes the following steps:
-... | ["[Filtration] From several liters of water (fresh or sea).\nCascade filtration on filters of different porosity with the help of a perilstatic pump.", "[Filtration] Pre-filtration through a 100µm celular sieve to remove large debris and larger zooplanktonic organisms (not of interest in our case).", "[Filtration] Succ... |
37,463 | Transformation Protocol for BL21(DE3) Competent Cells (C2527I) | 1 | dx.doi.org/10.17504/protocols.io.bgtxjwpn | https://www.protocols.io/view/transformation-protocol-for-bl21-de3-competent-cel-bgtxjwpn | New England Biolabs | TITLE: Transformation Protocol for BL21(DE3) Competent Cells (C2527I)
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This transformation protocol is for the C2527I cells. (For the C2527H protocol, see </span><a href="https://www.protocols.io/view/Transformation-P... | ["Thaw a tube of BL21(DE3) Competent E. coli cells until the last ice crystals disappear.\non ice", "Mix gently and carefully pipette into a transformation tube .\n[cells]\non ice", "Add – containing – to the cell mixture.\n1 µl\n5 µl\n1 pg\n[plasmid DNA]", "Carefully flick the tube 4–5 times to mix cells and DNA. Do... |
19,057 | Total protein extraction from adipose tissue and cells | null | dx.doi.org/10.17504/protocols.io.wurfev6 | null | Caroline Green | TITLE: Total protein extraction from adipose tissue and cells
AUTHORS: Caroline Green
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Adipose tissue, especially white adipose tissue (WAT), has been shown to be associated with endocrine and organ inflammation in addition to energy storage. Extraction... | ["Place buffer A, centrifuge column and receiver tube sleeve on ice for pre-cooling.", "Weigh 50-80 mg of fresh or frozen adipose tissue, place it on several layers of paper towels, squeeze with your thumb and forefinger, and remove a portion of the oil from the tissue. Place the tissue into the 1.5 ml centrifuge tube ... |
79,942 | Infection of Biomphalaria glabrata snails with Schistosoma mansoni miracidia | 4 | dx.doi.org/10.17504/protocols.io.36wgqjkkxvk5/v1 | https://www.protocols.io/view/infection-of-biomphalaria-glabrata-snails-with-sch-csbewaje | Sarah K Buddenborg | TITLE: Infection of Biomphalaria glabrata snails with Schistosoma mansoni miracidia
AUTHORS: Sarah K Buddenborg
[DESCRIPTION]
To infect Biomphalaria glabrata snails with miracidia hatched from Schistosoma mansoni eggs
[STEPS]
SECTION: Lung preparation
1. Collect lungs from patent mice into 50ml Falcon tubes containi... | ["[Lung preparation] Collect lungs from patent mice into 50ml Falcon tubes containing pre-warmed 37°C 1x DPBS", "[Lung preparation] Remove lungs from PBS and place in large mortar or laboratory blender", "[Lung preparation] Gently homogenise lung tissue", "[Lung preparation] Place homogenised lung slurry into a flask (... |
43,301 | COVID-19 ARTIC v3 Illumina library construction and sequencing protocol - high throughput 384 format | 1 | dx.doi.org/10.17504/protocols.io.bnidmca6 | https://www.protocols.io/view/covid-19-artic-v3-illumina-library-construction-an-bnidmca6 | DNA Pipelines R&D, Benjamin Farr, Diana Rajan, Emma Betteridge, Lesley Shirley, Michael Quail, Naomi Park, Nicholas Redshaw, Iraad Bronner, Louise Aigrain, Scott Goodwin, Scott Thurston, Stefanie Lensing, Carol Scott, Nicholas Salmon, Charlotte Beaver, Rachel Nelson, Alex Alderton, Ian Johnston | TITLE: COVID-19 ARTIC v3 Illumina library construction and sequencing protocol - high throughput 384 format
AUTHORS: DNA Pipelines R&D, Benjamin Farr, Diana Rajan, Emma Betteridge, Lesley Shirley, Michael Quail, Naomi Park, Nicholas Redshaw, Iraad Bronner, Louise Aigrain, Scott Goodwin, Scott Thurston, Stefanie Lensing... | ["[cDNA generation]\nImportant! This step must be performed in a RNase free, pre-PCR environment in which post PCR COVID-19 amplicons are not present, to minimise risk of sample contamination.Decontaminate bench surfaces, pipettes and gloves with RNase ZAP before starting work. Keep reagents and samples chilled through... |
87,631 | Sanger Tree of Life HMW DNA Fragmentation: Covaris g-TUBE for ULI PacBio | 4 | dx.doi.org/10.17504/protocols.io.q26g7pm81gwz/v1 | https://www.protocols.io/view/sanger-tree-of-life-hmw-dna-fragmentation-covaris-cztpx6mn | graeme oatley, Filipa Sampaio, Lucy Kitchin, Raquel Juliana Vionette do Amaral, Caroline Howard | TITLE: Sanger Tree of Life HMW DNA Fragmentation: Covaris g-TUBE for ULI PacBio
AUTHORS: graeme oatley, Filipa Sampaio, Lucy Kitchin, Raquel Juliana Vionette do Amaral, Caroline Howard
[DESCRIPTION]
This protocol describes the centrifugation-mediated fragmentation of HMW DNA from samples prepared via any of the Sanger... | ["[Laboratory protocol] Label the required number of Covaris g-TUBEs for each DNA sample that will be sheared; ensure that the tubes are labelled both on the lid and on the bottom.", "[Laboratory protocol] Prior to transferring the DNA sample from its original tube, first mix the DNA sample by pipetting carefully with ... |
null | null | null | dx.doi.org/10.17504/protocols.io.n75dhq6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol makes a master mix for PCR that is ready to load into an agarose gel. Useful for colony PCR and related.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
25,185 | Copy of Fluorescence analysis using CF imager-v2 | null | dx.doi.org/10.17504/protocols.io.4t9gwr6 | null | Steven Burgess | TITLE: Copy of Fluorescence analysis using CF imager-v2
AUTHORS: Steven Burgess
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. The surface of the leaf should be 140mm from the base of the imaging chamber, and can be adjusted by lowering or raising the plant under analysis. Position plant/leaf in the chamb... | ["The surface of the leaf should be 140mm from the base of the imaging chamber, and can be adjusted by lowering or raising the plant under analysis. Position plant/leaf in the chamber", "[Set exposure]\nMaually adjust the apeture as shown on the right to allow and optimal amount of light into the imager so as not to ov... |
69,072 | GeoMx-NGS Manual RNA Slide Preparation Protocol | 1 | dx.doi.org/10.17504/protocols.io.bp2l61ojkvqe/v1 | https://www.protocols.io/view/geomx-ngs-manual-rna-slide-preparation-protocol-cfpqtmmw | Angela R.S. Kruse, Morad C Malek, Jamie Allen, Melissa Farrow, Jeff Spraggins | TITLE: GeoMx-NGS Manual RNA Slide Preparation Protocol
AUTHORS: Angela R.S. Kruse, Morad C Malek, Jamie Allen, Melissa Farrow, Jeff Spraggins
[DESCRIPTION]
This protocol describes the preparation of tissue samples for spatial transcriptomics via the Nanostring GeoMx Digital Spatial Profiler.
Expected outcome: Tissu... | ["[PREPARE REAGENTS] Reagent Dilution Storage 95% EtOH Prepare 500 mL of 95% ethanol by adding 25 mL of DEPC- treated water to 475 mL of 100% ethanol. Change at least weekly. RT 1X PBS pH 7.4 Prepare 1 L of 1X PBS by combining 100 mL of 10X PBS and 900 mL of DEPC-treated water. Don't reuse. 4°C ... |
null | null | null | dx.doi.org/10.17504/protocols.io.r7yd9pw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>FSCI Reproducibilty workshop</p>
[STEPS]
?.
?.
?. | [] |
73,594 | Passaging cells in MultiFlasks | 4 | null | https://www.protocols.io/view/passaging-cells-in-multiflasks-cj42uqye | Allan JW Lui | TITLE: Passaging cells in MultiFlasks
AUTHORS: Allan JW Lui
[DESCRIPTION]
Simple protocol for working with Falcon Multi-Flasks
[STEPS]
SECTION: 5-layer Multi-Flask
1. Per flask, Prepare and pre-warm:
200ml PBS
30ml 0.25% Trypsin-EDTA or other detachment reagent
70ml complete media for trypsin inactivation, plus extra... | ["[5-layer Multi-Flask] Per flask, Prepare and pre-warm:\n200ml PBS\n30ml 0.25% Trypsin-EDTA or other detachment reagent\n70ml complete media for trypsin inactivation, plus extra for reseeding", "[5-layer Multi-Flask] Pour out media in flask, wash out remaining media twice by:\nAdding 100 mL\nDistributing equally and e... |
90,972 | Maintenance of hPSC | 4 | dx.doi.org/10.17504/protocols.io.bp2l6xmjrlqe/v1 | https://www.protocols.io/view/maintenance-of-hpsc-c434yyqw | Lyn Healy, Valeria Fernandez Vallone, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Lisa Pavinato, Fatma Visal FVO OKUR, Harald Stachelscheid | TITLE: Maintenance of hPSC
AUTHORS: Lyn Healy, Valeria Fernandez Vallone, Nathalie Lefort, Katarzyna Ludwik, Tamer Onder, Lisa Pavinato, Fatma Visal FVO OKUR, Harald Stachelscheid
[DESCRIPTION]
This protocol describes maintenance of established hPSC lines in expansion media on matrix coated culture vessels.
[GUIDELIN... | ["[Culture Monitoring] Inspect hPSC cultures daily using a microsope to monitor morphology, the presence of spontaneous differentiated cells, and confluence. Based on these observations, determine if the cultures require further action (e.g. removal of differentiated cells, passaging). Use reference images in the proto... |
93,920 | Pathogen-Oriented Low-cost Assembly & Re-sequencing (POLAR): A highly sensitive and high-throughput SARS-CoV-2 diagnostic based on whole genome sequencing | 1 | dx.doi.org/10.17504/protocols.io.3byl47xx8lo5/v2 | https://www.protocols.io/view/pathogen-oriented-low-cost-assembly-amp-re-sequenc-c7x8zprw | Per A. Adastra, Neva C. Durand, Namita Mitra, Saul Godinez, Ragini Mahajan, Alyssa Blackburn, Zane Colaric, Joshua W. M. Theisen, David Weisz, Olga Dudchenko, Andreas Gnirke, Suhas S.P. Rao, Parwinder Kaur, Erez Lieberman Aiden, Aviva Presser Aiden | TITLE: Pathogen-Oriented Low-cost Assembly & Re-sequencing (POLAR): A highly sensitive and high-throughput SARS-CoV-2 diagnostic based on whole genome sequencing
AUTHORS: Per A. Adastra, Neva C. Durand, Namita Mitra, Saul Godinez, Ragini Mahajan, Alyssa Blackburn, Zane Colaric, Joshua W. M. Theisen, David Weisz, Ol... | ["[cDNA preparation] Make a mastermix of the dNTPs and Random Hexamers for 96 samples (account for pipette error), pipette to mix and add 1 µL from mastermix to each well in a 96-well plate. \n\nTo each well, add 5.5 µL of RNA extract eluted in Step 7.", "[cDNA preparation] Set-up and run the following program on a the... |
31,077 | Confocal microscopy and characterization of synaptic boutons associated with ganglion neurons | null | dx.doi.org/10.17504/protocols.io.bakdics6 | https://www.protocols.io/view/confocal-microscopy-and-characterization-of-synapt-bakdics6 | Janet Keast, Peregrine Osborne | TITLE: Confocal microscopy and characterization of synaptic boutons associated with ganglion neurons
AUTHORS: Janet Keast, Peregrine Osborne
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes confocal microscopy and image analysis procedures for characterizing neuronal cell bodi... | ["[Confocal microscopy]\nUsing Zen Black (Zeiss software), batch process Airyscan output to 16-bit 1260 x 1260 pixel images.", "[Confocal microscopy]\nTo optimize visualization of synaptic boutons, image using a 60x 1.4 NA oil immersion objective with 3x digital zoom. Multi-channel images were collected by sequential i... |
null | null | null | dx.doi.org/10.17504/protocols.io.drt56m | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<span class="cit-title">This protocols is from:<br /></span><span class="cit-auth cit-auth-type-author">Linlin Yin</span><span class="cit-sep cit-sep-separator">, et al.</span><span class="cit-auth cit-auth-type-author"> (2015) <a href="http://www.genetics.org/content/200/2/431.... | [] |
14,955 | Immunofluorescence protocol of Pax7 and androgen receptor for frozen muscle sections with unmasking | 1 | dx.doi.org/10.17504/protocols.io.sujeeun | https://www.protocols.io/view/immunofluorescence-protocol-of-pax7-and-androgen-r-sujeeun | Hiroshi Sakai | TITLE: Immunofluorescence protocol of Pax7 and androgen receptor for frozen muscle sections with unmasking
AUTHORS: Hiroshi Sakai
[DESCRIPTION]
Immunofluorescence protocol of Pax7 on muscle tissues is a critical step for studying skeletal muscle regeneration. Here, I describe the simple protocol for Pax7 for isopentan... | ["[Fix] Fix the slides with 4% PFA/PBS for 5 min at room temperature.", "[Fix] Wash the slides in PBS three times for 5 min.", "[Unmasking] Place the slides into a slide chamber in PBS", "[Unmasking] Fill a beaker with 500 ml of Sodium citrate.", "[Unmasking] Place the beaker on the hotplate and turn it on full power."... |
66,208 | Extraction of bacterial DNA using MagMAX™ CORE Nucleic Acid Purification Kit on KingFisher™ Flex Instrument | 1 | dx.doi.org/10.17504/protocols.io.81wgb781ovpk/v2 | https://www.protocols.io/view/extraction-of-bacterial-dna-using-magmax-core-nucl-ccv8sw9w | Leyi Wang, Carol Maddox, Melanie Prarat, Yan Zhang, Lifang Yan, Akhilesh Ramachandran, Sai Sankara Narayanan, Girish Patil | TITLE: Extraction of bacterial DNA using MagMAX™ CORE Nucleic Acid Purification Kit on KingFisher™ Flex Instrument
AUTHORS: Leyi Wang, Carol Maddox, Melanie Prarat, Yan Zhang, Lifang Yan, Akhilesh Ramachandran, Sai Sankara Narayanan, Girish Patil
[DESCRIPTION]
This procedure is used to extract genome DNA of bacter... | ["Prepare plates for Robot", "Prepare Wash Plate 2 by adding 500 μL of MagMAX™ CORE Wash Solution 2", "Prepare Wash Plate 1 by adding 500 μL of MagMAX™ CORE Wash Solution 1", "Prepare Elution Plate by adding 100 μL of MagMAX™ CORE Elution Buffer", "Set Tip Comb in a 0.5 ml 96-Well Plate\n\n \n Plate setup of Proc... |
99,444 | Nebuloni, F. & Do, Q. B. et al. (2024) A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy | 2 | dx.doi.org/10.17504/protocols.io.36wgqjwwxvk5/v2 | https://www.protocols.io/view/nebuloni-f-amp-do-q-b-et-al-2024-a-fluid-walled-mi-ddcu22ww | Federico Nebuloni, Quyen Do, Richard Wade-Martins | TITLE: Nebuloni, F. & Do, Q. B. et al. (2024) A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy
AUTHORS: Federico Nebuloni, Quyen Do, Richard Wade-Martins
[DESCRIPTION]
This collection contains six protocols detailing methods used in Nebuloni, F. & Do, Q. B.et al. (2024) A fl... | [] |
62,119 | Superior Nutra Keto 100% Safe And Effective FEATURES Of Ingredients | 3 | dx.doi.org/10.17504/protocols.io.4r3l2o3kqv1y/v1 | https://www.protocols.io/view/superior-nutra-keto-100-safe-and-effective-feature-b8wfrxbn | SuperiorNutraKeto | TITLE: Superior Nutra Keto 100% Safe And Effective FEATURES Of Ingredients
AUTHORS: SuperiorNutraKeto
[DESCRIPTION]
Official Website - Click Here forSuperior Nutra Keto
Availability Of Superior Nutra Keto - Online On Website
Main Benefits - Fat & Weight Loss Without Any Side Effect
VISIT THE OFFICIAL WEBSITE OF PO... | [] |
79,819 | List of IHC/WB antibodies, RT-PCR probes and software used | 1 | dx.doi.org/10.17504/protocols.io.4r3l27ryjg1y/v1 | https://www.protocols.click/view/list-of-ihc-wb-antibodies-rt-pcr-probes-and-softwa-cr7jv9kn | carmela.giachino | TITLE: List of IHC/WB antibodies, RT-PCR probes and software used
AUTHORS: carmela.giachino
[DESCRIPTION]
This protocol details list of antibodies used for immunohistochemistry and western blot.
[STEPS]
SECTION: List of antibodies used for immunohistochemistry and western blot
1. Table 1. List of antibodies used for ... | ["[List of antibodies used for immunohistochemistry and western blot] Table 1. List of antibodies used for immunohistochemistry and western blot.\n\n \n Antibody name Company Dilution RRID CCR2, goat polyclonal Thermo Fisher Scientific 0.388889 AB_557978 CD11b, rabbit Abcam 0.736111 AB_2650514... |
41,531 | Qbiotix protocol | 1 | dx.doi.org/10.17504/protocols.io.bks3kwgn | https://www.protocols.io/view/qbiotix-protocol-bks3kwgn | vito | TITLE: Qbiotix protocol
AUTHORS: vito
[STEPS]
?. 1- collect saliva vial
1 mL
?. 2- open vial and scan barcode
?. 3- dispense sample to single well in plate with a pipette
250 µl
?. 4- dispense of magnetic bead solution in well with a pipette
250 µl
?. 5- incubate at controlled temperature on the plate reader
?. 6... | ["1- collect saliva vial\n1 mL", "2- open vial and scan barcode", "3- dispense sample to single well in plate with a pipette\n250 µl", "4- dispense of magnetic bead solution in well with a pipette\n250 µl", "5- incubate at controlled temperature on the plate reader", "6- place plate on magnetic separator", "7- inse... |
null | null | null | dx.doi.org/10.17504/protocols.io.n8tdhwn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is for the extraction and purification of up to 10ug of plasmid DNA. It can be used for the purification of plasmid DNA ranging from 40 bp to 40kbp. </p>
[BEFORE_START]
<p>1) Add RNAse A solution to the bottle containing Solution 1 and mix well. Once RNAse A so... | [] |
57,729 | Thawing of feeder-free hPSCs | 4 | dx.doi.org/10.17504/protocols.io.b4k9quz6 | https://www.protocols.io/view/thawing-of-feeder-free-hpscs-b4k9quz6 | Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner | TITLE: Thawing of feeder-free hPSCs
AUTHORS: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes the procedure of thawing feeder-free human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex
General Notes
Throughout this protocol, the term hPSC is u... | ["Prepare one 6-well VTN/Matrigel/Geltrex-coated plate for each vial of frozen hPSCs. \n\nA detailed protocol on \"Coating plates\" can be found in the \"Feeder-free culturing of hPSCs\" collection. A link to this collection can be found in the title section of this protocol, located above", "Centrifuge 200-300 x g, 5 ... |
42,101 | AMAS to Concatenate Sequences | 5 | dx.doi.org/10.17504/protocols.io.j8nlk45ewg5r/v1 | https://www.protocols.io/view/amas-to-concatenate-sequences-bmcvk2w6 | Dakota Betz | TITLE: AMAS to Concatenate Sequences
AUTHORS: Dakota Betz
[DESCRIPTION]
Brief instructions on how to use the AMAS Package to concatenate sequences in the command line. Especially useful if your SequenceMatrix is no longer compatible with your version of Java.
[STEPS]
SECTION: Installation
1. Prerequisites: Python 3 ... | ["[Installation] Prerequisites: Python 3 previously installed.\nDownload the AMAS package here:\n \nOnce downloaded, drag the AMAS folder into your Applications folder. You will need the path to this folder in order to proceed.", "[Concatenate] Use the command line to navigate to the folder containing your aligned file... |
98,926 | Optimizing Genetic Modification in Agrobacterium rhizogenes K599 through Electroporation Method | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj3e4lx1/v1 | https://www.protocols.io/view/optimizing-genetic-modification-in-agrobacterium-r-dcun2wve | Mariana López-Sámano, Kalpana Nanjareddy, Manoj-Kumar Arthikala | TITLE: Optimizing Genetic Modification in Agrobacterium rhizogenes K599 through Electroporation Method
AUTHORS: Mariana López-Sámano, Kalpana Nanjareddy, Manoj-Kumar Arthikala
[DESCRIPTION]
Electroporation has emerged as a highly effective method for swiftly and proficiently introducing exogenous plasmid DNA into vari... | ["[Preinoculum preparation] Begin by inoculating 10 ml of LB medium with a single colony of Agrobacterium rhizogenes K599 from a freshly streaked plate. Incubate the culture overnight at 28°C with agitation at 180 rpm, which typically takes approximately 12 hours.", "[Preparation of Electrocompetent cells] Inoculate 10... |
51,131 | d3 29/6 | 1 | null | https://www.protocols.io/view/d3-29-6-bv63n9gn | Mariia Guliakina II | TITLE: d3 29/6
AUTHORS: Mariia Guliakina II
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">test</div></div>
[STEPS]
?. test | ["test"] |
88,031 | Viral DNA and RNA Extraction from Faecal Specimens using QIAamp MinElute Virus Kits (Spin-Column Approach) | 4 | dx.doi.org/10.17504/protocols.io.q26g7pmkqgwz/v1 | https://www.protocols.io/view/viral-dna-and-rna-extraction-from-faecal-specimens-cz77x9rn | Naluepanat Yodjan | TITLE: Viral DNA and RNA Extraction from Faecal Specimens using QIAamp MinElute Virus Kits (Spin-Column Approach)
AUTHORS: Naluepanat Yodjan
[DESCRIPTION]
The QIAamp MinElute Virus Spin procedure comprises four steps (lyse, bind, wash, elute) and is carried out using QIAamp MinElute columns in a standard microcentrifu... | ["Faecal specimens were vigorously vortexed and homogenised for 5 min.", "The supernatants were collected after centrifugation at 15,000 x g for 10 min.", "Approximately 200 µl of the supernatant from each sample was filtered through a 0.45 µm filter and 0.2 µm filter to remove eukaryotic cell and bacterium-sized parti... |
30,768 | Anti-Neu5Gc Antibody Kit Protocol - Flow Cytometry | null | dx.doi.org/10.17504/protocols.io.baaqiadw | null | Sam Li | TITLE: Anti-Neu5Gc Antibody Kit Protocol - Flow Cytometry
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Anti-Neu5Gc may be used for staining cells prior to analysis by Flow Cytometry. This kit contains all the essential components needed to identify Neu5Gc on the surface of cells b... | ["Three (3) tubes containing cells to be examined. If choosing to use different dilutions of primary antibody, as noted in step 3, more tubes may be needed.Three (3) tubes containing positive control cells.Three (3) tubes containing negative control cells.", "Each set of tubes to be analyzed should receive an antibody ... |
45,069 | Quantifying Reactive Oxygen Species in diatoms | 4 | dx.doi.org/10.17504/protocols.io.ewov14qopvr2/v1 | https://www.protocols.io/view/quantifying-reactive-oxygen-species-in-diatoms-bp9mmr46 | Phoebe Argyle, Jana Hinners, Nathan G. Walworth, Sinéad Collins, Naomi M. Levine, Martina A. Doblin | TITLE: Quantifying Reactive Oxygen Species in diatoms
AUTHORS: Phoebe Argyle, Jana Hinners, Nathan G. Walworth, Sinéad Collins, Naomi M. Levine, Martina A. Doblin
[DESCRIPTION]
This protocol us designed to assess the relative concentration of reactive oxygen species in diatoms using a fluorescent dye.
This protocol ... | ["[Initiation of assay] Remove algae cultures from growth conditions/incubator.\n\nTransfer 2 x 500 µL aliquots of microalgae culture into separate wells of a 48-well tissue culture plate. \n \n\n One well will act as a blank, the other as the treatment. Do this for all cultures being assayed.", "[Initiation of assay]... |
85,958 | Quick guide to use paceTOMO for cryo-ET data collection from Titan Krios | 5 | dx.doi.org/10.17504/protocols.io.6qpvr3442vmk/v1 | https://www.protocols.io/view/quick-guide-to-use-pacetomo-for-cryo-et-data-colle-cx7exrje | Josh Hutchings, Siyu Chen | TITLE: Quick guide to use paceTOMO for cryo-ET data collection from Titan Krios
AUTHORS: Josh Hutchings, Siyu Chen
[DESCRIPTION]
This quick guide provides key minimal steps for preparing the Titan/SerialEM for tomogram data collection on lamella or in vitro specimens with K3 camera. paceTOMO routine is also included f... | ["[paceTOMO data collection workflow] Initial configuration of Record settings:", "[paceTOMO data collection workflow] Move stage to hole position.", "[Preparation before performing alignment] Do inventory in TEM UI once all temperatures shown on the screen are <100K. If not already, load the cross-grating grid onto th... |
92,919 | Extraction and analysis of primary metabolites during Xanthomonas-Barley interaction | 1 | null | https://www.protocols.io/view/extraction-and-analysis-of-primary-metabolites-dur-c6yxzfxn | Veronica Roman-Reyna, Nathaniel Heiden, Jules Butchacas, Hannah Toth, Jessica L. Cooperstone, Jonathan M. Jacobs | TITLE: Extraction and analysis of primary metabolites during Xanthomonas-Barley interaction
AUTHORS: Veronica Roman-Reyna, Nathaniel Heiden, Jules Butchacas, Hannah Toth, Jessica L. Cooperstone, Jonathan M. Jacobs
[DESCRIPTION]
Intercellular host-associated bacteria shape the chemistry of the living eukaryotic environ... | ["[Sample preparation] Sample preparation, you need Barley cv. Morex seeds, Xanthomonas translucens pv. undulosa strain UPB513, and Xanthomonas translucens pv. translucent strain UPB886.\n\nThe experiment requires samples for\nBarley plants\nThe Barley cv. Morex was used for in-planta studies. All plants were sown at ... |
30,981 | HuBMAP Tissue Sectioning for FFPE Specimens | null | dx.doi.org/10.17504/protocols.io.bahdib26 | null | Leigh Propper, Marda Jorgensen | TITLE: HuBMAP Tissue Sectioning for FFPE Specimens
AUTHORS: Leigh Propper, Marda Jorgensen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This method details our microtomy (sectioning) process for reasearch specimens involved with HuBMAP.</div><div class = "text-block">This process follows the micr... | ["[Tissue Block Preparation]\nEnsure the tissue blocks you have chosen to section have been correctly embedded, and all wax has been scraped/cleaned off the sides of the block.Ensure the tissue blocks have been correctly labeled with proper identification (case #, type, etc)", "[Microtome Preparation]\nLocate your tiss... |
49,147 | How bad is the mere presence of a phone? A replication of Przybylski and Weinstein (2013) and an extension to creativity, PLoS ONE 16(6), 2021 | 1 | dx.doi.org/10.17504/protocols.io.bt83nryn | https://www.protocols.io/view/how-bad-is-the-mere-presence-of-a-phone-a-replicat-bt83nryn | Claire Linares, Anne-Laure Sellier | TITLE: How bad is the mere presence of a phone? A replication of Przybylski and Weinstein (2013) and an extension to creativity, PLoS ONE 16(6), 2021
AUTHORS: Claire Linares, Anne-Laure Sellier
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>A 2013 article reported two experiments suggestin... | ["[Lab sessions: Preparation of the study room and manipulation]\nPreparation of the room layoutThe study rooms have two tables, one with the smartphone or notebook and the second one for the creativity tasks.Please see the schemas for the layout of the rooms.Figure 2. Layout of the study rooms during the conversation ... |
56,052 | Modified NEBNext® VarSkip Short SARS-CoV-2 Library Prep Kit for Illumina Platforms - adapted for wastewater samples | 1 | dx.doi.org/10.17504/protocols.io.b2yuqfww | https://www.protocols.io/view/modified-nebnext-varskip-short-sars-cov-2-library-b2yuqfww | Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim | TITLE: Modified NEBNext® VarSkip Short SARS-CoV-2 Library Prep Kit for Illumina Platforms - adapted for wastewater samples
AUTHORS: Padmini Ramachandran, Tamara Walsky, Amanda Windsor, Maria Hoffmann, Chris Grim
[DESCRIPTION]
PURPOSE:
This method was developed at the FDA’s Center for Food Safety and Applied Nu... | ["[cDNA Synthesis] Gently mix and spin down the LunaScript RT SuperMix reagent. Prepare the cDNA synthesis reaction as described below:\n \n\nFor no template controls, mix the following components:", "[cDNA Synthesis] Incubate reactions in a thermocycler* with the following steps:", "[Targeted cDNA Amplification]", "[T... |
97,095 | USDA LTAR Common Experiment measurement: Saturated hydraulic conductivity | 0 | dx.doi.org/10.17504/protocols.io.eq2lywz1qvx9/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-saturated-da3f2gjn | Adam P. Schreiner-McGraw, Claire Baffaut | TITLE: USDA LTAR Common Experiment measurement: Saturated hydraulic conductivity
AUTHORS: Adam P. Schreiner-McGraw, Claire Baffaut
[DESCRIPTION]
The saturated hydraulic conductivity (Ksat) represents the speed at which a fluid can move through a porous medium, and it is a fundamental parameter that governs water flow ... | ["[Data collection] There is no standard approach to determining the saturated hydraulic conductivity, but the most common method is using single-ring or double-ring infiltrometers (Bouwer, 1986). \n\nDouble-ring infiltrometers were developed with the idea that the outer ring would prevent water infiltrating from the i... |
49,209 | HuBMAP UF TMC - FACS Sorting of Live CD45+ Cells for 10x scRNASeq | 1 | dx.doi.org/10.17504/protocols.io.buaznsf6 | https://www.protocols.io/view/hubmap-uf-tmc-facs-sorting-of-live-cd45-cells-for-buaznsf6 | Maigan Brusko | TITLE: HuBMAP UF TMC - FACS Sorting of Live CD45+ Cells for 10x scRNASeq
AUTHORS: Maigan Brusko
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This standard operating procedure (SOP) provides instructions for staining and sorting live cells. This SOP applies to cryopreserved cells that are stained ... | ["[Thaw Cryopreserved Cells]\na. In a 15 mL conical tube, warm 10 mL of cDMEM in a bead bath set to 37 o Cb. Thaw the cells in the cryovials in a water bath set at 37 o Cc. Immediately, resuspend the cells in 10 ml of RT cDMEM i. First, add 1 mL of cDMEM to the cryovial ii. Then, transfer the cells from the cryov... |
75,104 | LEGACY01: STATISTICS AND DATA ANALYSIS | 1 | null | https://www.protocols.io/view/legacy01-statistics-and-data-analysis-cmj8u4rw | Katrina M Pollock, Calliope Dendrou | TITLE: LEGACY01: STATISTICS AND DATA ANALYSIS
AUTHORS: Katrina M Pollock, Calliope Dendrou
[DESCRIPTION]
This protocol details about statistics and data analysis in an experimental medicine study of seasonal influenza vaccination responses in Lymph nodE single-cell Genomics in AnCestrY (LEGACY01).
[GUIDELINES]
STATIS... | [] |
37,911 | Protocol for Exo-CIP™ Rapid PCR Cleanup (#E1050) | 1 | dx.doi.org/10.17504/protocols.io.bg9xjz7n | https://www.protocols.io/view/protocol-for-exo-cip-rapid-pcr-cleanup-e1050-bg9xjz7n | New England Biolabs | TITLE: Protocol for Exo-CIP™ Rapid PCR Cleanup (#E1050)
AUTHORS: New England Biolabs
[DESCRIPTION]
Exo-CIP™ Rapid PCR Cleanup Kit
Rapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplification
Reaction complete in 4 minutes
Thermolabile formulation can be heat inactivated in 1 minute at 8... | ["Transfer 5 µL to a new PCR tube and add 1 µL and 1 µL. The final volume is 7 µL.", "Mix thoroughly and briefly centrifuge at 1000 x g.", "Incubate the reaction tube for 4 min at 37 °C followed by 1 min at 80 °C.", "Submit 3 µL or less* for sequencing using BigDye™ Terminator v3.1 Cycle Sequencing Kit or store the tr... |
83,880 | Processing human frontal cortex brain tissue for population-scale Oxford Nanopore long-read DNA sequencing SOP | 4 | dx.doi.org/10.17504/protocols.io.kxygx3mqkg8j/v1 | https://www.protocols.click/view/processing-human-frontal-cortex-brain-tissue-for-p-cv6gw9bw | Kimberley J Billingsley, Ramita Dewan, Laksh Malik, Pilar Alvarez Jerez, Stith Kiley, Cornelis Blauwendraat, on behalf of the CARD Long-read Team | TITLE: Processing human frontal cortex brain tissue for population-scale Oxford Nanopore long-read DNA sequencing SOP
AUTHORS: Kimberley J Billingsley, Ramita Dewan, Laksh Malik, Pilar Alvarez Jerez, Stith Kiley, Cornelis Blauwendraat, on behalf of the CARD Long-read Team
[DESCRIPTION]
Processing human frontal cortex ... | ["Part 1: Brain Tissue Cutting (~2.5 hours for 16 samples)", "Add dry ice to a ice bucket", "Place supplies (sterile weigh boat, razor blade, labeled empty 2mL protein LoBind microcentrifuge tubes and cooling block) in dry ice and allow to chill for ~ 5 min", "Obtain tissue samples from -80 °Cfreezer and place in dry i... |
58,667 | Mouse Heart Perfusion - Ultrastructural Analysis (Using Karnovsky's Fixative) | 4 | dx.doi.org/10.17504/protocols.io.4r3l2o7k3v1y/v1 | https://www.protocols.io/view/mouse-heart-perfusion-ultrastructural-analysis-usi-b5ijq4cn | Mark Ellisman, Eric Bushong, Keun-Young Kim, Maryann Martone | TITLE: Mouse Heart Perfusion - Ultrastructural Analysis (Using Karnovsky's Fixative)
AUTHORS: Mark Ellisman, Eric Bushong, Keun-Young Kim, Maryann Martone
[DESCRIPTION]
Optimal fixation for mouse tissue analysis.
[STEPS]
SECTION: Preparing Karnovsky’s Modified Fixative Solution
1. For 100 ml of 2.0% paraformalde... | ["[Preparing Karnovsky’s Modified Fixative Solution] For 100 ml of 2.0% paraformaldehyde - 2.5% glutaraldehydein 0.15M sodium cacodylate buffer, pH 7.4 with 2mM CaCl2:", "[Preparing Karnovsky’s Modified Fixative Solution] Add 2 g prills paraformaldehyde into 125ml Erlenmeyer flask. (Paraformaldehyde, Prills: EMS cat. #... |
null | null | null | dx.doi.org/10.17504/protocols.io.e9pbh5n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>ezRAD is from the family of RAD (restriction site associated DNA) techniques that cuts up the DNA (either by sonication or restriction enzymes) and looks at large areas of the genome, as apposed to the whole genome. RADseq data often forms stacks of DNA on the same loci (vert... | [] |
18,997 | Protocol for evaluation of normal hearing criteria | null | dx.doi.org/10.17504/protocols.io.wsvfee6 | null | Kelly Cristina Lira de Andrade, Thamyres Ataíde Bezerra Verçosa, Aline Tenório Lins Carnaúba, Pedro de Lemos Menezes | TITLE: Protocol for evaluation of normal hearing criteria
AUTHORS: Kelly Cristina Lira de Andrade, Thamyres Ataíde Bezerra Verçosa, Aline Tenório Lins Carnaúba, Pedro de Lemos Menezes
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The present protocol aims to perform the screening of the subjects o... | [] |
69,024 | Operative Wound Care in Total Knee Arthroplasty | 1 | dx.doi.org/10.17504/protocols.io.bp2l61o5kvqe/v1 | https://www.protocols.io/view/operative-wound-care-in-total-knee-arthroplasty-cfm8tk9w | Luisa Mululo, Fabricio Loures, Marcia Vanzillota, José Mauricio Moraes do Carmo, Marcelo Campos | TITLE: Operative Wound Care in Total Knee Arthroplasty
AUTHORS: Luisa Mululo, Fabricio Loures, Marcia Vanzillota, José Mauricio Moraes do Carmo, Marcelo Campos
[DESCRIPTION]
Guidelines for surgical wound care for patients undergoing total knee arthroplasty surgery performed at Hospital Universitario Pedro Ernesto.
... | [] |
64,262 | Anesthetic, analgesic and antibiotic protocol | 1 | dx.doi.org/10.17504/protocols.io.e6nvwkqm2vmk/v1 | https://www.protocols.io/view/anesthetic-analgesic-and-antibiotic-protocol-cazesf3e | geoffrey.pages | TITLE: Anesthetic, analgesic and antibiotic protocol
AUTHORS: geoffrey.pages
[DESCRIPTION]
This protocol was used to manage dogs with cruciate ligament disease treated by TPLO
[STEPS]
SECTION: Premedication (using acepromazine)
1. acepromazine (Calmivet; Vetoquinol™, Lure, France), 0.05 mg/kg intramuscularly (IM)
S... | ["[Premedication (using acepromazine)] acepromazine (Calmivet; Vetoquinol™, Lure, France), 0.05 mg/kg intramuscularly (IM)", "[Premedication]", "[Premedication (using diazepam)] diazepam (Valium; Roche™, Boulogne-Billancourt, France), 0.25 mg/kg intravenously (IV)", "[Premedication (both)] morphine (Morphine; Lavoisier... |
96,163 | Transforming pJC8 into HB101 | 0 | dx.doi.org/10.17504/protocols.io.n2bvj3kkwlk5/v1 | https://www.protocols.io/view/transforming-pjc8-into-hb101-c96bz9an | Bonnie Evans | TITLE: Transforming pJC8 into HB101
AUTHORS: Bonnie Evans
[DESCRIPTION]
Taken from Mix and Go E. coli Transformation Kit (see attachment).
Transforming E. coli HB101 with pJC8-empty cosmid to use as a control strain.
[STEPS]
SECTION: Before starting
1. Prepare tetracycline LB agar plates
SECTION: Before starting
... | ["[Before starting] Prepare tetracycline LB agar plates", "[Before starting] Warm plates in 37 °C incubator", "[Before starting] Prepare SOC medium", "[Before starting] Get SOB medium and 20 % glucose from media kitchen", "[Before starting] Add 2 mL 20 % glucose to 100 ml SOB medium", "[Transformation] Isolate pJC8 cos... |
47,407 | Lumbar Puncture | 4 | dx.doi.org/10.17504/protocols.io.q26g78x11lwz/v1 | https://www.protocols.io/view/lumbar-puncture-bsipncdn | Clemens Scherzer, Bradley Hyman, Charles Jennings | TITLE: Lumbar Puncture
AUTHORS: Clemens Scherzer, Bradley Hyman, Charles Jennings
[DESCRIPTION]
This protocol explains the Standard Operating Protocol for performing a Lumbar Puncture.
[BEFORE_START]
*Optimum time delay between withdrawal and freezing should be within 1-2 hours per European consensus.
Ref:
[GU... | ["[Lumbar Puncture] Label the collection tubes with the sample ID as appropriate.", "[Lumbar Puncture] Place aliquot tubes on dry ice prior to procedure so they are pre-cooled.", "[Lumbar Puncture] Perform lumbar puncture using the atraumatic technique, inserting the needle with the bevel in parallel to the dura fibers... |
null | null | null | dx.doi.org/10.17504/protocols.io.sszeef6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is used to clarity the process of the short insert size WGS libraries preparation for the L. maculatus.</p>
[STEPS]
?.
?.
?.
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.m68c9hw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocal characterized each focal fish (western mosquitofish, <em>Gambusia affinis</em>) for three standard indicators of personality using well-established experimental approaches: (1) boldness as latency to emerge from shelter and enter an unknown area, (2) activity in... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.h9zb976 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
<p> </p>
<p>Adapted from the original publication Rippka, R., DeRulles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. Generic assignments, strain histories... | [] |
56,848 | Preparation of LRRK1 RCKW cryo-EM grids | 4 | dx.doi.org/10.17504/protocols.io.b3rqqm5w | https://www.protocols.io/view/preparation-of-lrrk1-rckw-cryo-em-grids-b3rqqm5w | Mariusz Matyszewski, David Snead | TITLE: Preparation of LRRK1 RCKW cryo-EM grids
AUTHORS: Mariusz Matyszewski, David Snead
[DESCRIPTION]
Protocol used to create LRRK1 RCKW grids for cryo-EM used in Snead, Matyszewski, Dickey et al.
[BEFORE_START]
Decide which protein concentration to use, and create the proper LRRK1 buffers in order to obtain the ri... | ["[Freezing Grids] Plasma clean grids.\nWe used UltrAuFoil Holey Gold 1.2/1.3 300 mesh grids and plasma cleaned them in the Solarus II (Gatan) using the QuantiFoil Au preset.", "[Freezing Grids] Apply protein to grids and plunge freeze.\nWe used a Vitrobot (FEI) to blot away excess sample and plunge freeze", "[Freezing... |
35,391 | Tissue Dissociation and Nuclei Isolation | null | dx.doi.org/10.17504/protocols.io.bes7jehn | null | Paul Oyler-Castrillo, Chiara Gerhardinger, Juliana Brown | TITLE: Tissue Dissociation and Nuclei Isolation
AUTHORS: Paul Oyler-Castrillo, Chiara Gerhardinger, Juliana Brown
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol details a step in the workflow for our contribution to the BRAIN Initiative Cell Census Network (BICCN) – Co... | ["[Preparation]\nPrepare materials and reagents for the experiment. If there alre multiple people carrying out the experiment, Steps 1.2, 1.3, and 1.4 can be done while someone else carries out Step 2. Step 3 onward will require the NSB (Step 1.2) and NSB+Ruby (Step 1.3) solutions.", "[Preparation]\nPre-chill the centr... |
99,984 | 384 Well PicoGreen | 1 | dx.doi.org/10.17504/protocols.io.36wgq5ekxgk5/v2 | https://www.protocols.io/view/384-well-picogreen-ddvq265w | Allen Institute for Brain Science | TITLE: 384 Well PicoGreen
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
Quant-iT™ PicoGreen® dsDNA Assay is used for detection and quantitation of double stranded DNA products.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes o... | [] |
47,318 | Expression and purification of recombinant human Parkin and pSer65 Parkin | 4 | dx.doi.org/10.17504/protocols.io.bsfwnbpe | https://www.protocols.io/view/expression-and-purification-of-recombinant-human-p-bsfwnbpe | Michael Stevens, Miratul M. K. Muqit | TITLE: Expression and purification of recombinant human Parkin and pSer65 Parkin
AUTHORS: Michael Stevens, Miratul M. K. Muqit
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Mutations in PARK2 encoding Parkin are causal for early-onset Parkinson’s disease. Parkin is a ubiquitin E3 ligase and is act... | ["[Transformation of plasmid into competent bacteria]\nMix with . Coli and incubate for .\n[6His-SUMO-Parkin plasmid stock (~50 ng/\\ul)]\n[competent BL21 DE3 pLysS Codon Plus E]\non ice", "[Transformation of plasmid into competent bacteria]\nHeat shock cells by incubation in a heat block equilibrated at for ... |
81,031 | Donor Selection Criteria for Human Liver Procurement -- University of Minnesota Human TMC | 1 | dx.doi.org/10.17504/protocols.io.q26g7yen1gwz/v1 | https://www.protocols.click/view/donor-selection-criteria-for-human-liver-procureme-ctdfwi3n | Sayeed Ikramuddin, Laura Niedernhofer | TITLE: Donor Selection Criteria for Human Liver Procurement -- University of Minnesota Human TMC
AUTHORS: Sayeed Ikramuddin, Laura Niedernhofer
[DESCRIPTION]
This document outlines the inclusion and exclusion criteria for donors of liver and blood for the SenNet Consortium program from the University of Minnesota Huma... | ["[Inclusion Criteria] Age 18 years old or older", "[Inclusion Criteria] Undergoing abdominal surgical procedure with general anesthesia. \n\nLaparoscopic procedures to include/consider cholecystectomy, bariatric surgery, hernia repair (incisional or hiatal) in which the liver is accessible, esophageal surgery, or GI r... |
52,581 | Far East XL - Does (Far East XL Male Enhancement) Is Really Works Or Scam? | 1 | dx.doi.org/10.17504/protocols.io.bxkdpks6 | https://www.protocols.io/view/far-east-xl-does-far-east-xl-male-enhancement-is-r-bxkdpks6 | health | TITLE: Far East XL - Does (Far East XL Male Enhancement) Is Really Works Or Scam?
AUTHORS: health
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><a href="https://www.healthpills24x7.com/order-new-flow-xl-me" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;font-weigh... | ["Product Name: Far East XL Male EnhancementOfficial Website:Click here To Order Far East XL Male Enhancement From Its Official WebsiteLow libido and lack of confidence in bed can be common problems for men over 40. As the age starts to increase, your sexual prowess will naturally decrease. Even today, doctors still... |
32,039 | Workflow for retrieving all the data of the analysis introduced in the article "Citing and referencing habits in Medicine and Social Sciences journals in 2019" | 1 | dx.doi.org/10.17504/protocols.io.bbifikbn | https://www.protocols.io/view/workflow-for-retrieving-all-the-data-of-the-analys-bbifikbn | Erika Alves dos Santos, Silvio Peroni, Marcos Luiz Mucheroni | TITLE: Workflow for retrieving all the data of the analysis introduced in the article "Citing and referencing habits in Medicine and Social Sciences journals in 2019"
AUTHORS: Erika Alves dos Santos, Silvio Peroni, Marcos Luiz Mucheroni
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol ... | ["[Obtaining SCImago database journals title list]\nFirst, a search should be conducted in order to retrieve the titles of the journals indexed by SCImago database, according to the description in figure 1:\n\t\t\t\t\t\t\t .justify:after {\n\t\t\t\t\t\t\t content: \"\";\n\t\t\t\t\t\t\t display:inline-block;\n\t\... |
null | null | null | dx.doi.org/10.17504/protocols.io.ddc22v | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is a protocol for high yield recovery of pure DNA from agarose gels using the Zymoclean™ Gel DNA Recovery Kit.
[BEFORE_START]
Add 24 ml 100% ethanol (26 ml 95% ethanol) to the 6 ml <strong>DNA Wash Buffer</strong> concentrate. Add 96 ml 100% ethanol (104 ml 95% ethan... | [] |
23,885 | Neuropathy Phentoyping Protocols - Animal Perfusion/Tissue Fixation | null | dx.doi.org/10.17504/protocols.io.3jmgkk6 | null | Eva Feldman | TITLE: Neuropathy Phentoyping Protocols - Animal Perfusion/Tissue Fixation
AUTHORS: Eva Feldman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block"><span style = "font-weight:bold;">Phenotyping of Rodents for the Presence o... | ["Intracardiac perfusion: For intracardiac perfusion, the fixative may be administered by peristaltic pump or by gravity. The Morphology Core uses gravity. 1. Fix bottles are placed at a height of 1 meter above the animal. 2. The animal is placed on an open metal grid over a large tray to catch the fixative. NO FIXATIV... |
32,360 | MojoSort™ Mouse CD8a Selection Kit Column Protocol | null | dx.doi.org/10.17504/protocols.io.bbugintw | null | Sam Li | TITLE: MojoSort™ Mouse CD8a Selection Kit Column Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simp... | ["Prepare cells from your tissue of interest or blood without lysing erythrocytes.", "In the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4mL in a 5 mL (12 x 75 mm) polypropylene tube. Note: Keep MojoSort™ Buffer on ice throughout the procedure.", "Filter the cells with... |
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