id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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null | null | null | dx.doi.org/10.17504/protocols.io.sr2ed8e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Extraction of Phycocyanin from Synechocystis liquid culture.</p>
[STEPS]
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52,980 | Macherey-Nagel Nucleospin 96 Food protocol for bee pollen | 4 | dx.doi.org/10.17504/protocols.io.kxygxpbrol8j/v1 | https://www.protocols.io/view/macherey-nagel-nucleospin-96-food-protocol-for-bee-bxyuppww | Lauren Ponisio, Jocelyn Zorn | TITLE: Macherey-Nagel Nucleospin 96 Food protocol for bee pollen
AUTHORS: Lauren Ponisio, Jocelyn Zorn
[DESCRIPTION]
Macherey-Nagel Nucleospin 96 Food protocol for bee pollen
[STEPS]
1. UV sterilize supplies for 2 96 well plates worth of extractions: 4 50mL centrifuge tubes, 2 15mL centrifuge tubes, zirconia beads, ... | ["UV sterilize supplies for 2 96 well plates worth of extractions: 4 50mL centrifuge tubes, 2 15mL centrifuge tubes, zirconia beads, 2 96 deep well plates and clear strip caps, 2 s-blocks, 2 96 well elution plates, 14 1000uL tip boxes, 2 200uL tip boxes, 2 10uL tip boxes, 6 reagent troughs, and 6 96 well microplates", ... |
92,896 | Further Micro-scaled MEDI (Macronutrient Extraction and Determination from Invertebrates) | 6 | dx.doi.org/10.17504/protocols.io.8epv5zp36v1b/v2 | https://www.protocols.io/view/further-micro-scaled-medi-macronutrient-extraction-c6x8zfrw | Jordan P Cuff | TITLE: Further Micro-scaled MEDI (Macronutrient Extraction and Determination from Invertebrates)
AUTHORS: Jordan P Cuff
[DESCRIPTION]
Macronutrients, comprising carbohydrates, proteins and lipids, underpin many ecological processes, but their quantification in ecological studies is often inaccurate and laborious, requ... | ["[Welcome to MEDI!] Welcome to Macronutrient Extraction and Determination from Invertebrates (further micro-scaled edition)! \n\nThe following presents a micro-scaled version of the original MEDI protocol. The further micro-scaled protocol is intended particularly for invertebrates of dry volumes of 1 mg or less which... |
null | null | null | dx.doi.org/10.17504/protocols.io.fysbpwe | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div class="page" title="Page 1">
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<p>The SensiFAST™ Probe Hi-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. The kit has been formulated for us... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.kjicuke | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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39,142 | 2XYT Medium (Version 2-0) Haseloff Lab | 4 | dx.doi.org/10.17504/protocols.io.bigekbte | https://www.protocols.io/view/2xyt-medium-version-2-0-haseloff-lab-bigekbte | Fernando Guzman Chavez | TITLE: 2XYT Medium (Version 2-0) Haseloff Lab
AUTHORS: Fernando Guzman Chavez
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:center"><span style = "font-weight:bold;">Work instruction for 2xYT and 2xYTG medium preparation</span></div></div><div class = "te... | ["[2xYT and 2xYTG medium preparation]\n.justify:after {\n\t\t\t\t\t\t\t content: \"\";\n\t\t\t\t\t\t\t display:inline-block;\n\t\t\t\t\t\t\t width: 100%;\n\t\t\t\t\t\t\t }\n\t\t\t\t\t\t\tFor this media, following compounds have to be ready-to-use:2XYT powder1M K2HPO4solution1M KH2PO4solution", "[2xYT and 2xYT... |
25,258 | Purifying DNA from an Agarose Gel | null | dx.doi.org/10.17504/protocols.io.4wigxce | null | Addgene The Nonprofit Plasmid Repository | TITLE: Purifying DNA from an Agarose Gel
AUTHORS: Addgene The Nonprofit Plasmid Repository
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is for purifying DNA from an agarose gel. To see the full abstract and additional resources, please visit </span><a href="https://www.addgene... | ["Follow the agarose gel electrophoresis protocol with the following amendments: Gel purification is most efficient with lower % agarose gels, so you will want to stay in the 0.7-0.8% range if possible.You will want nice crisp bands. This can be achieved by using a wider gel comb and running the gel at a lower voltage... |
103,538 | Dendritic spine analysis | 0 | dx.doi.org/10.17504/protocols.io.e6nvw1879lmk/v1 | https://www.protocols.io/view/dendritic-spine-analysis-dhcs32we | Chuyu Chen | TITLE: Dendritic spine analysis
AUTHORS: Chuyu Chen
[DESCRIPTION]
Haloperidol is reported to induce a series of homeostatic synaptic and intrinsic adaptations primarily in the indirect pathway circuits. These adaptations include dendritic spine loss in iSPNs, which occurs after 5 days and persists for up to 14 days of... | ["[Stereotaxic Surgeries] P4-5-day-old LRRK2GS/WT-Adora2aCre pups were cryoanesthetized and received ketoprofen for analgesia", "[Stereotaxic Surgeries] Pups were placed on a cooling pad on a stereotaxic frame", "[Stereotaxic Surgeries] 200 nl of the AAV-DIO-EGFP virus (7×10¹² vg/mL) were delivered into the dorsal stri... |
47,125 | Multiplexed RT-qPCR to screen for SARS-COV-2 B.1.1.7, B.1.351, and P.1 variants of concern | 1 | dx.doi.org/10.17504/protocols.io.br9vm966 | https://www.protocols.io/view/multiplexed-rt-qpcr-to-screen-for-sars-cov-2-b-1-1-br9vm966 | Chantal Vogels, Joseph Fauver, Nathan Grubaugh | TITLE: Multiplexed RT-qPCR to screen for SARS-COV-2 B.1.1.7, B.1.351, and P.1 variants of concern
AUTHORS: Chantal Vogels, Joseph Fauver, Nathan Grubaugh
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify">With the emergence of SARS-CoV-2 variants that... | ["[RT-qPCR Protocol]\nBriefly vortex and centrifuge reagents before use.", "[RT-qPCR Protocol]\nPrepare 20 µM working stocks of the primers and probes, by adding 20 µL of 100 µM stock to 80 µL nuclease-free water.", "[RT-qPCR Protocol]\nUse the 20 µM working stocks to prepare primer-probe-water mix containing the follo... |
102,998 | AAV Production and Purification | 0 | dx.doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v1 | https://www.protocols.io/view/aav-production-and-purification-dgtw3wpe | Justin T Savage | TITLE: AAV Production and Purification
AUTHORS: Justin T Savage
[DESCRIPTION]
This protocol is for the production and purification of Adeno-Associated Virus. The protocol contains the necessary steps to produce AAVs from HEK293T cell cultures.
[STEPS]
SECTION: Growing HEK 293T Cells: Day 1 -Morning
1. Make Media for... | ["[Growing HEK 293T Cells: Day 1 -Morning] Make Media for HEK293T Cells", "[Growing HEK 293T Cells: Day 1 -Morning] Thawing HEK293 Cells", "[Growing HEK 293T Cells: Day 1 -Morning] Thaw HEK293T Cells in 10cm Dish with 10 ml HEK Medium (One Vial into three 10cm Dish)", "[Day 3 -Morning] Split HEK293T Cells (Usually beco... |
86,453 | Glucose Tolerance Test | 1 | null | https://www.protocols.io/view/glucose-tolerance-test-cynvxve6 | Sabina Marciano, Roberta Marongiu | TITLE: Glucose Tolerance Test
AUTHORS: Sabina Marciano, Roberta Marongiu
[DESCRIPTION]
Glucose tolerance test is performed to determine how quickly the glucose is cleared from the blood. It is used to test for diabetes or insulin resistance.
[STEPS]
1. Single cage the mice for one week in advance
2. Weigh the mice
... | ["Single cage the mice for one week in advance", "Weigh the mice", "Fast the animals for 360 min", "After fasting, measure the glucose with a glucose meter", "Perform an Intraperitoneal (IP) injection with 2 g/kg body weight of glucose (20% D-glucose stock solution dissolving 2g of glucose in 10ml saline and give 10ul ... |
28,978 | Fluorescent in vitro model to assess invasion and intracellular matruation of Bd in A6 cells (Plos One) | null | dx.doi.org/10.17504/protocols.io.8ishuee | null | Elin Verbrugghe | TITLE: Fluorescent in vitro model to assess invasion and intracellular matruation of Bd in A6 cells (Plos One)
AUTHORS: Elin Verbrugghe
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The largest current disease-induced loss of vertebrate biodiversity is due to chytridiomycosis and despite the... | ["Prepare Cell Medium A: L15 medium: 70%Distilled water: 20%Fetal bovine serum 10%", "Prepare Cell Medium B: L15 medium: 40%Distilled water: 55%Fetal bovine serum: 5%", "Coat coverslips with Rat tail collagen: Add glass coverslips in a 24-well tissue culture plate. Coat the glass coverslips at 37°C for 2 hours. Therefo... |
44,645 | SensingSelf S1 Rapid Antigen Test (Saliva/Sputum/Stool) | 4 | dx.doi.org/10.17504/protocols.io.bpudmns6 | https://www.protocols.io/view/sensingself-s1-rapid-antigen-test-saliva-sputum-st-bpudmns6 | Shripal Gandhi, Santo Purnama, Keyur Patel, Praveen Sukumara, Dr Rinu R Ravi | TITLE: SensingSelf S1 Rapid Antigen Test (Saliva/Sputum/Stool)
AUTHORS: Shripal Gandhi, Santo Purnama, Keyur Patel, Praveen Sukumara, Dr Rinu R Ravi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">The S1 COVID-19 Rapid ... | ["[SALIVA SAMPLE COLLECTION]\nSaliva should be collected with the assistance of a healthcare worker or technician.", "[SALIVA SAMPLE COLLECTION]\nBefore collection, clean hands using alcohol-based sanitizer or soap and water (no fragrances) and wear appropriate PPE (at minimum, gloves and a mask).", "[SALIVA SAMPLE COL... |
null | null | null | dx.doi.org/10.17504/protocols.io.is5ceg6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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59,858 | Immunocytochemistry of motor neurons derived from iPSCs with the hNIL construct protocol | 4 | dx.doi.org/10.17504/protocols.io.6qpvr68bovmk/v1 | https://www.protocols.io/view/immunocytochemistry-of-motor-neurons-derived-from-b6psrdne | Maria Sckaff, Carissa Feliciano, Zachary Nevin, Bruce Conklin, Claire D Clelland | TITLE: Immunocytochemistry of motor neurons derived from iPSCs with the hNIL construct protocol
AUTHORS: Maria Sckaff, Carissa Feliciano, Zachary Nevin, Bruce Conklin, Claire D Clelland
[DESCRIPTION]
This protocol describes the immunocytochemistry for staining motor neurons derived from induced pluripotent stem cell... | ["[Immunocytochemistry of the hNIL motor neurons: Day 1: Fixing, Permeabilizing, Blocking and Coating Cells with Primary Antibody] Without removing media from the wells, add 4% PFA on all target wells and let it sit for 30 min at Room temperature (the volume of 4% PFA should equal the volume of media already in the wel... |
null | null | null | dx.doi.org/10.17504/protocols.io.ebgbajw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
Both the iMicrobe (<a href="http://imicrobe.us" target="_blank">http://imicrobe.us</a>) and iVirus (<a href="http://ivirus.us" target="_blank">http://ivirus.us</a>) sites accept user data via an FTP (file transfer protocol) site (<a href="ftp://ftp.imicrobe.us" target="_blank">f... | [] |
47,228 | DRAGEN COVID Lineage App SARS-CoV-2 Strain Characterization on the Illumina BaseSpace Platform | 5 | null | https://www.protocols.io/view/dragen-covid-lineage-app-sars-cov-2-strain-charact-bsc4nayw | Technical Outreach and Assistance for States Team | TITLE: DRAGEN COVID Lineage App SARS-CoV-2 Strain Characterization on the Illumina BaseSpace Platform
AUTHORS: Technical Outreach and Assistance for States Team
[DESCRIPTION]
This protocol provides instructions on how to run the DRAGON COVID Lineage app on the Illumina BaseSpace Sequence Hub. The DRAGON COVID Lineage... | ["[Create Basespace Project] Login to the Illumina BaseSpace Platform and create a new BaseSpace project", "[Add Fastq files to BaseSpace Project] Add Fastq files to the new project directory. This can be done by either uploading fastq files from a local directory or by importing sequences using their Sequence Read Arc... |
null | null | null | dx.doi.org/10.17504/protocols.io.ibecaje | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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82,036 | Adding 1-hydroxyphenazine to L4 C. elegans in liquid culture | 4 | dx.doi.org/10.17504/protocols.io.yxmvm28png3p/v1 | https://www.protocols.io/view/adding-1-hydroxyphenazine-to-l4-c-elegans-in-liqui-cucuwsww | Muhammad Zaka Asif, Man Shah, Yosef Smadi | TITLE: Adding 1-hydroxyphenazine to L4 C. elegans in liquid culture
AUTHORS: Muhammad Zaka Asif, Man Shah, Yosef Smadi
[DESCRIPTION]
This protocol describes day 4 of our workflow to grow worms in liquid culture to induce the production of natural products (in this case, 1-HP derivatives). In this protocol, we add the ... | ["Follow steps 1-6 from the protocol for transferring C. elegans to S-buffer, but centrifuge at 525 RCF for 1 minute", "Dilute to a final concentration of 30,000 worms/mL in S-basal and final toxin concentration of 22.3 µM 1-HP.", "Repeat steps 1-2 for 1.1% DMSO and bacteria-only controls.", "If using negative controls... |
null | null | null | dx.doi.org/10.17504/protocols.io.j8gcrtw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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62,368 | SenseSmarter Brain Booster+ : Boost Brain Health And Help Users Remain Focused! | 3 | dx.doi.org/10.17504/protocols.io.kqdg3p7x1l25/v1 | https://www.protocols.io/view/sensesmarter-brain-booster-boost-brain-health-and-b858ry9w | H A | TITLE: SenseSmarter Brain Booster+ : Boost Brain Health And Help Users Remain Focused!
AUTHORS: H A
[DESCRIPTION]
There is way too much discretion afforded physicians to code up whatever they want on claim forms such that two physicians seeing the exact same patient might code up different procedures and diagnostics ... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.rtcd6iw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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?. | ["For RNA extraction, 6 female third instar wandering larvae were homogenised in TRI reagent (Sigma) in a Precellys 24 homogeniser (Bertin Technologies, île-de-France, France).", "RNA was extracted with the standard TRI reagent protocol.", "1.5 µg of total RNA was treated with DNase I Amplification Grade (Sigma).", "cD... |
71,577 | Zeiss AxioImagerM.2 Apotome 2 Guide | 1 | null | https://www.protocols.io/view/zeiss-axioimagerm-2-apotome-2-guide-ch5zt876 | Condon ND | TITLE: Zeiss AxioImagerM.2 Apotome 2 Guide
AUTHORS: Condon ND
[DESCRIPTION]
This is a training guide for the Institute for Molecular Bioscience Microscopy Core Facility's Zeiss AxioImagerM.2 Apotome 2 (Upright) microscope.
More information about the system is available here: https://imb.uq.edu.au/microscopy-fluoro-2... | ["[System Start-up] Check the microscope to be sure it is clean. You are responsible for the condition of the microscope at the close of your session.If you find it dirty, make sure microscopy staff know about it; that is, email, phone us or by leaving feedback on the sheets provided. \nCheck the eyepiece dioptre setti... |
70,165 | easyDB – Circularization of rv0678 for genotypic bedaquiline resistance testing of Mycobacterium tuberculosis | 1 | dx.doi.org/10.17504/protocols.io.3byl4j24rlo5/v1 | https://www.protocols.io/view/easydb-circularization-of-rv0678-for-genotypic-bed-cgrvtv66 | Jason D Limberis | TITLE: easyDB – Circularization of rv0678 for genotypic bedaquiline resistance testing of Mycobacterium tuberculosis
AUTHORS: Jason D Limberis
[DESCRIPTION]
We designed primers with a tail sequence that forms a six-nucleotide hairpin at temperature <55oC, but not ≥55oC. These primers contain six phosphorothioate bonds... | ["[Prepare Buffers] ISO buffer (2.5X) Volume (ul) 1M Tris-HCl pH 7.5 100 200mM MgCl2 50 100mM dGTP 2 100mM dATP 2 100mM dTTP 2 100mM dCTP 2 100mM DTT 100 PEG 8000 50mg 50 mM NAD 20 H20 90 \n \n Master Mix Volume (ul) 2.5X ISO buffer 640 T7 exonuclease (10 U/μl)... |
49,520 | Nuclei Isolation from Tissue for 10x Multiome | 4 | null | https://www.protocols.io/view/nuclei-isolation-from-tissue-for-10x-multiome-bukqnuvw | Annika Weimer, Minyi Shi, Michael P Snyder | TITLE: Nuclei Isolation from Tissue for 10x Multiome
AUTHORS: Annika Weimer, Minyi Shi, Michael P Snyder
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Nuclei Isolation from Tissue for 10x Multiome</div></div>
[STEPS]
?. [Before you start the protocol:]
1) All steps should be performed on ice or a... | ["[Before you start the protocol:]\n1) All steps should be performed on ice or at 4°C. Pre-chill a swinging bucket centrifugeand a fixed angle centrifuge to 4°C.2) Pre-chill all Dounces and pestles to 4°C in a fridge.3) Pre-chill all tubes. For each sample you are processing, you will need:a. One 2 ml round-bottom LoBi... |
50,590 | Classifying dog breeds | 1 | dx.doi.org/10.17504/protocols.io.bvm6n49e | https://www.protocols.io/view/classifying-dog-breeds-bvm6n49e | Edgar Andrade-Lotero, Robert Goldstone, Javier Alejandro Velazco Garcia | TITLE: Classifying dog breeds
AUTHORS: Edgar Andrade-Lotero, Robert Goldstone, Javier Alejandro Velazco Garcia
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In this experiment we created the conditions for the emergence of a division in a cognitive task, mediated by language, instantiated in an i... | ["Instructions for the experiment", "Training rounds (all conditions)Player must classify five dogs into two breeds of one kind (either Terriers or Hounds).", "Classification", "Feedback", "Game roundsPlayer must classify five dogs into their appropriate breeds, but this time dogs come from both kinds (Terriers and Hou... |
89,895 | Detection of knockdown resistance mutations in Musca domestica by rhPCR | 4 | dx.doi.org/10.17504/protocols.io.eq2lyj9dqlx9/v1 | https://www.protocols.io/view/detection-of-knockdown-resistance-mutations-in-mus-c32fyqbn | Alden Estep, Neil Sanscrainte | TITLE: Detection of knockdown resistance mutations in Musca domestica by rhPCR
AUTHORS: Alden Estep, Neil Sanscrainte
[DESCRIPTION]
This protocol details the step-by-step procedure for assessing Musca domestica knockdown resistance (kdr) mutations using RNAse H2 PCR (rhPCR). This procedure utilizes the specificity of ... | ["[Reagent Preparation] Label 9 - 1.7ml microcentrifuge tubes (as 1-9) to prepare allele specific rhPCR reactions.", "[Sample preparation] Room temperature Add cubic zirconium beads to Omni Products 96-deep well plate using Biospec bead loader", "[Sample preparation] Room temperature Add 400 µL of deionized water using... |
null | null | null | dx.doi.org/10.17504/protocols.io.fg8bjzw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is the hands-on interactive component to the ECOGEO Workshop Module on Binning.</p>
[BEFORE_START]
<p>This tutorial is tailored for <a href="http://anvio.org" target="_blank">Anvi’o</a> v2.0.1. If you do not have this version available to you, certain things may not wor... | [] |
70,666 | Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765) | 1 | dx.doi.org/10.17504/protocols.io.6qpvredyblmk/v2 | https://www.protocols.io/view/protocol-for-use-with-nebnext-poly-a-mrna-magnetic-cg9itz4e | New England Biolabs, Isabel Gautreau | TITLE: Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765)
AUTHORS: New England Biolabs, Isabel Gautreau
[DESCRIPTION]
The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzyme... | ["[Probe Hybridization to RNA] Prepare the First Strand Synthesis Reaction Buffer and Random Primer Mix (2X) in a nuclease-free microcentrifuge tube as follows: \n\n \nYou can prepare the first strand synthesis reaction buffer later in the protocol, but it is important that it is ready before the elution in step 38. Th... |
30,659 | Immunohistochemistry Protocol for Paraffin-Embedded Sections | null | dx.doi.org/10.17504/protocols.io.97bh9in | null | Sam Li | TITLE: Immunohistochemistry Protocol for Paraffin-Embedded Sections
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Prepare formalin-fixed, paraffin-embedded tissue sections (steps 1-8):]
Fix freshly dissected tissue (less than 3 mm thick) with 10% formalin or other fixatives for 24-48 hou... | ["[Prepare formalin-fixed, paraffin-embedded tissue sections (steps 1-8):]\nFix freshly dissected tissue (less than 3 mm thick) with 10% formalin or other fixatives for 24-48 hour at room temperature. Caution: Formalin is a suspected carcinogen. It can cause eye, skin, and respiratory tract irritation. It should be han... |
25,248 | Marchantia spores production in Microboxes | null | dx.doi.org/10.17504/protocols.io.4v8gw9w | null | Eftychis Frangedakis | TITLE: Marchantia spores production in Microboxes
AUTHORS: Eftychis Frangedakis
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Spore sterilisation</div><div class = "text-block"><span>Marchantia spores can be obtained usually in three months using Microboxes. The lid of Microboxes has a specially d... | ["Put 15 “Jiffy 7” pellets in a Microbox, add 800 mL of water, close the lid and autoclave (A and B in Figure).", "After autoclaving the Microbox with the “Jiffy 7” pellets, work in a flow hood.", "Put at least one 10 mm x 10 mm thallus fragment or 3 gemmae per pellet using sterile tweezers (C and D in Figure).", "Add ... |
70,645 | Expansion microscopy with R1441C LRRK2 MEF cells: visualization of Myc-RILPL1 and TMEM55B | 4 | dx.doi.org/10.17504/protocols.io.ewov1o8m7lr2/v1 | https://www.protocols.io/view/expansion-microscopy-with-r1441c-lrrk2-mef-cells-v-cg8vtzw6 | Chloe A Hecht, Shahzad S. Khan, Sreeja Nair, Claire Y Chiang, Suzanne R Pfeffer | TITLE: Expansion microscopy with R1441C LRRK2 MEF cells: visualization of Myc-RILPL1 and TMEM55B
AUTHORS: Chloe A Hecht, Shahzad S. Khan, Sreeja Nair, Claire Y Chiang, Suzanne R Pfeffer
[DESCRIPTION]
Expansion microscopy is a super-resolution imaging technique that uses expandable hydrogels to increase the physical di... | ["[Transfection of Myc-RILPL1 in LRRK2 R1441C MEF cells] Seed LRRK2 R1441C MEF cells at 50-60% confluency on 12 mm glass coverslips in a 24 well plate in 500 µL of complete DMEM (DMEM containing 10% FBS and 1% penicillin-streptomycin) 24 hours before transfection.", "[Transfection of Myc-RILPL1 in LRRK2 R1441C MEF cell... |
45,488 | 10X-CITEseq protocol (COVID-19 patient samples +/- tetramer stain) | 1 | dx.doi.org/10.17504/protocols.io.bqnqmvdw | https://www.protocols.io/view/10x-citeseq-protocol-covid-19-patient-samples-tetr-bqnqmvdw | Yang Sun, David Lee, George Hartoularos, Jimmie Ye | TITLE: 10X-CITEseq protocol (COVID-19 patient samples +/- tetramer stain)
AUTHORS: Yang Sun, David Lee, George Hartoularos, Jimmie Ye
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;">Purpose</span></div></div><div clas... | ["[Antibody Reconstitution]\nTake two TotalSeq-C Human Panel vials from fridge.\n4 °C\nBriefly centrifuge each tube before opening.", "[Antibody Reconstitution]\nReconstitute each vial in .\n[cell staining buffer]", "[Antibody Reconstitution]\nVortex for - or until the solution visually looks resuspended.", "[Antibody... |
61,934 | Peptide Drug Screening | 6 | dx.doi.org/10.17504/protocols.io.6qpvr6mr2vmk/v1 | https://www.protocols.io/view/peptide-drug-screening-b8qnrvve | Cathy Miller | TITLE: Peptide Drug Screening
AUTHORS: Cathy Miller
[DESCRIPTION]
Peptides are ideally suited to mimic natural ligands and usually act in an antagonistic or agonistic manner. Small active peptide molecules have huge potential to be developed as vaccines, diagnostic reagentsand lead compounds. Therefore, peptides have... | [] |
108,972 | 3D Microfluidic Platforms for Extracellular Vesicle Isolation, Characterization, Downstream Analysis and In Vitro Treatment | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qzqpl1y/v2 | https://www.protocols.io/view/3d-microfluidic-platforms-for-extracellular-vesicl-dnnk5dcw | Emeli Chatterjee, Scott Lindsay, Tyler Ostrander, Marta Garcia-Contreras, Hawa S. Ndiaye, John Sauld, Gautam Mahajan, Prabhleen Singh, Saumya Das, Priyadarshini Pantham | TITLE: 3D Microfluidic Platforms for Extracellular Vesicle Isolation, Characterization, Downstream Analysis and In Vitro Treatment
AUTHORS: Emeli Chatterjee, Scott Lindsay, Tyler Ostrander, Marta Garcia-Contreras, Hawa S. Ndiaye, John Sauld, Gautam Mahajan, Prabhleen Singh, Saumya Das, Priyadarshini Pantham
[DESCRIPTI... | ["Organ-on-a-chip", "Liver-on-chip:\nFollow Manufacturer’s protocol for seeding chip with hepatocytes, Kupffer cells,\nStellate cells, and liver endothelial\ncells (Emulate, inc.).\nKidney-on-chip:\nFollow Manufacturer’s protocol for seeding chip with RPTECs and RMVECs\n(Emulate, inc.).", "Treatment of cells with extr... |
35,207 | Opentrons COVID-19 testing (Randox/qPCR common path, stations A & B, 24 samples) | null | dx.doi.org/10.17504/protocols.io.bemfjc3n | null | Max Marrone | TITLE: Opentrons COVID-19 testing (Randox/qPCR common path, stations A & B, 24 samples)
AUTHORS: Max Marrone
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Opentrons and the Open Medicine Institute are developing an automated high-throughput COVID-19 testing protocol to submit to the FDA for an Eme... | ["[Station A: Initial OT-2 setup]\nClean the Station A OT-2.", "[Station A: Initial OT-2 setup]\nStart pre-cooling the Temperature Module to . This is used to actively cool the internal extraction control RNA that will be added to each sample.\n4 °C", "[Station A: Initial OT-2 setup]\nPlace the following labware on the... |
93,844 | Sequencing-based Neutralization Assay for Influenza A Virus | 4 | dx.doi.org/10.17504/protocols.io.kqdg3xdmpg25/v1 | https://www.protocols.io/view/sequencing-based-neutralization-assay-for-influenz-c7vuzn6w | Andrea N. Loes, Rosario Araceli L Tarabi, Jesse Bloom | TITLE: Sequencing-based Neutralization Assay for Influenza A Virus
AUTHORS: Andrea N. Loes, Rosario Araceli L Tarabi, Jesse Bloom
[DESCRIPTION]
Traditional neutralization assays for influenza virus test a single viral strain against a single serum sample in each measurement. Here we describe a sequencing-based approac... | ["[(DAY 1) Determine your plate setup] Different plate setups may be used depending on how many samples of sera you are testing, and how many dilutions you wish to run for each serum. For example, you could perform serial dilutions of serum down the plate vertically, running up to 12 serum samples with 7 different dilu... |
61,539 | 70% Ethanol Fixation | 4 | null | https://www.protocols.io/view/70-ethanol-fixation-b8cbrssn | Arnold Federico | TITLE: 70% Ethanol Fixation
AUTHORS: Arnold Federico
[DESCRIPTION]
This protocol is specifically written for fixing/permeabilizing suspension cell culture in a final volume of 1mL. For DNA content analysis, such as for cell cycle analysis, a precipitating fixative such as ethanol is preferred over formaldehyde fixati... | ["[Protocol] Transfer 1 * 106 to 5 * 106 suspended cells to appropriate centrifuge tube and pellet by spinning at 300g for 5 minutes", "[Protocol] Discard supernatant without disturbing pellet. Resuspend and wash cells in 1mL 1X PBS with gentle pipetting. Spin down cells again at 300g for 5 minutes.", "[Protocol] While... |
null | null | null | dx.doi.org/10.17504/protocols.io.etbbein | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<a href="https://www.neb.com/products/m0302-t7-endonuclease-i" target="_blank">T7 Endonuclease I</a> recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonucleas... | [] |
86,144 | HannerLab Qubit Protocol | 1 | null | https://www.protocols.io/view/hannerlab-qubit-protocol-cyc8xszw | klindsay | TITLE: HannerLab Qubit Protocol
AUTHORS: klindsay
[DESCRIPTION]
The Qubit can measure the concentration of DNA (in ng/µL).
• Qubit HS (High sensitivity) is used for samples with low traces (0.2-100ng) of DNA (ie. eDNA)
• Qubit BR (Broad range) is used for samples with higher traces of DNA (2-1000ng) (ie. Tissue)
... | ["Prepare Qubit Buffer and Dye mix for the number of samples + 2 (for standards) + 1 extra.", "•\tSolution will be 199 parts buffer and 1 part dye (e.g., 199uL buffer for every 1uL dye)", "•\t(# of samples + 3 volumes)", "Vortex buffer-dye mix, then aliquot 198uL buffer-dye mix into each Qubit® tube for each of your sa... |
62,278 | Enriched Rat Housing | 4 | dx.doi.org/10.17504/protocols.io.261gen2oyg47/v1 | https://www.protocols.io/view/enriched-rat-housing-b83eryje | Keita Ishiwari, David Dietz, Jerry B Richards, Gabriel J. J. Barrero, Oksana Polesskaya, Abraham Palmer | TITLE: Enriched Rat Housing
AUTHORS: Keita Ishiwari, David Dietz, Jerry B Richards, Gabriel J. J. Barrero, Oksana Polesskaya, Abraham Palmer
[DESCRIPTION]
Description and benefits of post-weaning “Enriched Rat Housing” used in conjunction with various experiments
[STEPS]
SECTION: Standard housing
1. In "standa... | ["[Standard housing] In \"standard\" housing, after weaning, rats are housed in same-sex pairs in clear plastic laboratory cages (42 × 22 × 20 cm) lined with bedding (Aspen Shavings).", "[Enrichment housing] The environmentally enriched home cage consists of a large metal wire cage (90 × 60 × 120 cm; Doctors Forrest an... |
90,819 | GST fusion protein production | 1 | dx.doi.org/10.17504/protocols.io.4r3l22y14l1y/v1 | https://www.protocols.io/view/gst-fusion-protein-production-c4xbyxin | Leonardo A Parra-Rivas | TITLE: GST fusion protein production
AUTHORS: Leonardo A Parra-Rivas
[DESCRIPTION]
GST fusion protein production
[STEPS]
1. Full-length recombinant human WT α-syn, α-syn S129A, and S129D were expressed in Escherichia coli BL21 (DE3) (New England Biolabs, Cat # C2530H) using the bacterial expression vector pGEX-KGmyc.... | ["Full-length recombinant human WT α-syn, α-syn S129A, and S129D were expressed in Escherichia coli BL21 (DE3) (New England Biolabs, Cat # C2530H) using the bacterial expression vector pGEX-KGmyc. Following transformation protein expression was induced with 0.05 mM IPTG (isopropyl-β-d-thiogalactopyranoside),and either ... |
80,054 | Tissue Sectioning Guidelines (Fresh Frozen)- CODEX/Phenocycler-Fusion | 4 | null | https://www.protocols.io/view/tissue-sectioning-guidelines-fresh-frozen-codex-ph-csewwbfe | Santhosh Sivajothi | TITLE: Tissue Sectioning Guidelines (Fresh Frozen)- CODEX/Phenocycler-Fusion
AUTHORS: Santhosh Sivajothi
[DESCRIPTION]
The purpose of this SOP is to outline the techniques for sectioning and storage of fresh-frozen tissue samples for PhenoCycler-Fusion experiments
[GUIDELINES]
Fresh-frozen tissue sections are mounted... | ["[PREPARE CRYOSTAT CHAMBER] Standard cryostats with temperature control are recommended for tissue sectioning. Most tissues are sectioned in temperatures ranging from -15°C to -25°C. The exact temperature is unique to each tissue type and should be determined according to standard sectioning procedures.", "[FRESH-FROZ... |
null | null | null | dx.doi.org/10.17504/protocols.io.rurd6v6 | null | null | TITLE: No Title
AUTHORS:
[STEPS] | [] |
108,067 | Record visually-evoked eye movements in head-fixed adult mice using a hemispherical projection system | 0 | dx.doi.org/10.17504/protocols.io.4r3l2qjbjl1y/v1 | https://www.protocols.io/view/record-visually-evoked-eye-movements-in-head-fixed-dmsb46an | Scott C. Harris, Rose B. Creed, Alexandra Nelson | TITLE: Record visually-evoked eye movements in head-fixed adult mice using a hemispherical projection system
AUTHORS: Scott C. Harris, Rose B. Creed, Alexandra Nelson
[DESCRIPTION]
This protocol describes how to record visually-evoked eye movements in head-fixed adult mice using a hemispherical projection system.
... | ["[Rig Setup] Cover the concave surface of the acrylic hemisphere with an even coat of the UV paint.", "[Rig Setup] Using optical posts, secure the hemisphere with several inches of clearance above the breadboard/air table - there must be sufficient space for the project to fit underneath the hemisphere.", "[Rig Setup... |
28,852 | Streaking and Isolating Bacteria on a LB Agar Plate | null | dx.doi.org/10.17504/protocols.io.8euhtew | null | Priota Islam | TITLE: Streaking and Isolating Bacteria on a LB Agar Plate
AUTHORS: Priota Islam
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Follow this protocol if you have a glycerol stock of the bacteria of interest and want to isolate an individual clonal population (single colony) of bacteria from this sto... | ["Obtain an LB agar plate with appropriate antibiotic if any.", "Label the bottom of the plate with the strain name and the date. It is also a good idea to add the antibiotic resistance (if any) and your initials.", "Sterilize your lab bench by spraying it down with 70% ethanol and wiping it down with a paper towel. Ma... |
62,541 | TruKeto Reviews Scam Exposed Honest Customer Results? | 1 | dx.doi.org/10.17504/protocols.io.3byl4bxrovo5/v1 | https://www.protocols.io/view/truketo-reviews-scam-exposed-honest-customer-resul-b9bmr2k6 | wepixo , H H, health , H A | TITLE: TruKeto Reviews Scam Exposed Honest Customer Results?
AUTHORS: wepixo , H H, health , H A
[DESCRIPTION]
https://www.eastbaytimes.com/2022/05/12/truketo-reviews-shark-tank-warning-tru-keto-pills-price-legit-website-for-sale/
[STEPS]
1. TruKetoLosing weight and overseeing down your extra muscle to fats sum h... | ["TruKetoLosing weight and overseeing down your extra muscle to fats sum has by no means, been expressly fundamental. In any case, with such a huge load of weight decline structures drifting around, it'll common be direct one which interminably works helpful. Which is the explanation such vast people are shuddering to ... |
90,667 | QUINT Workflow for Fluorescence | 4 | dx.doi.org/10.17504/protocols.io.4r3l22y6jl1y/v1 | https://www.protocols.io/view/quint-workflow-for-fluorescence-c4sjywcn | Michael X. Henderson | TITLE: QUINT Workflow for Fluorescence
AUTHORS: Michael X. Henderson
[DESCRIPTION]
This protocol describes QUINT workflow for fluorescence.
[GUIDELINES]
Purpose
The purpose of this workflow is to enable mouse brain segmentation, registration, and quantification of regional signals. The simplest segmentation is done ... | ["[QuPath Visualization/Segmentation] Open the QuPath application. Select ‘Create project’.", "[QuPath Visualization/Segmentation] Select your slide/stain folder within your QUINT Workflow project folder (i.e., slide24stain19).", "[QuPath Visualization/Segmentation] Select ‘Add images’ > ‘Choose files’. Navigate to the... |
null | null | null | dx.doi.org/10.17504/protocols.io.cqfvtm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is a generic PNGase F protocol with denaturing reaction conditions. It is appropriate for both <a href="https://www.neb.com/products/p0704-pngase-f" target="_blank">P0704</a> and <a href="https://www.neb.com/products/p0708-pngase-f-recombinant" target="_... | [] |
41,255 | test 31.08 | 1 | null | https://www.protocols.io/view/test-31-08-bkifkubn | Mariia | TITLE: test 31.08
AUTHORS: Mariia
[STEPS]
?. test
?. Of friendship on inhabiting diminution discovered as. Did friendly eat breeding building few nor. Object he barton no effect played valley afford. Period so to oppose we little seeing or branch. Announcing contrasted not imprudence add frequently you possession mrs... | ["test", "Of friendship on inhabiting diminution discovered as. Did friendly eat breeding building few nor. Object he barton no effect played valley afford. Period so to oppose we little seeing or branch. Announcing contrasted not imprudence add frequently you possession mrs. Period saw his houses square and misery. Ho... |
null | null | null | dx.doi.org/10.17504/protocols.io.iqjcdun | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes how to calculate the hyperemic myocardial blood flow, a clinically critical parameter, with CZT-based SPECT and a single-scan rest/stress protocol that is proposed in our manuscript.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
81,294 | RNA Slide Preparation Protocol (FFPE) for nanostring DSP - GeoMx | 4 | null | https://www.protocols.io/view/rna-slide-preparation-protocol-ffpe-for-nanostring-ctmnwk5e | Nicolas Martin | TITLE: RNA Slide Preparation Protocol (FFPE) for nanostring DSP - GeoMx
AUTHORS: Nicolas Martin
[DESCRIPTION]
This protocol is designed for RNA slide preparation for formalin-fixed tissue.
[GUIDELINES]
IMPORTANT:
Take care to maintain nuclease-free conditions. The most significant risk of contamination comes from G... | ["Prepare reagents\n\nPrepare the reagents using the dilution instructions (see Table 1).\nUse DEPC- treated water for all dilutions. The actual volume of\nreagents used in the protocol will vary – the volumes to prepare in Table 1 are suggestions.\n\nTable 1: Reagent prep for RNA slide preparation\n\n Reagent ... |
33,104 | Protocol for nuclear extraction from human heart tissue for single cell sequencing | null | dx.doi.org/10.17504/protocols.io.bcjqiumw | null | Christian Pfleger, Shin Lin | TITLE: Protocol for nuclear extraction from human heart tissue for single cell sequencing
AUTHORS: Christian Pfleger, Shin Lin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is for nuclear extraction from human heart tissue for single cell sequencing.</div></div>
[STEPS]
?. [On dry i... | ["[On dry ice]\nPut on dry ice:flat bottom mortar and pestle, hammer and foreceps sample-flash frozen heart tissue scale plate", "[In laminar air hood – on dry ice]\nPulverize tissue in mortar using pestle and hammer.", "[In laminar air hood – on wet ice]\nTransfer pulversized tissue in 6 cm dish containing .\n[cell ly... |
71,466 | H&E | 1 | dx.doi.org/10.17504/protocols.io.36wgqj79kvk5/v1 | https://www.protocols.io/view/h-amp-e-ch2it8ce | emonte | TITLE: H&E
AUTHORS: emonte
[DESCRIPTION]
H&E
[STEPS]
1. H&E staining protocol for pathology lab is here.
| ["H&E staining protocol for pathology lab is here."] |
null | null | null | dx.doi.org/10.17504/protocols.io.h8bb9sn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This collection of protocols describes the preparation of reagents for transfecting DNA into the choanoflagellate <em>Salpingoeca rosetta</em> using a Nucleofector 4d Device and SF kit from Lonza. Plasmids that use <em>S. rosetta</em> promoters and regulatory elements to driv... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hveb63e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Manufacturer's protocol</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
94,448 | Direct acid extraction | 1 | dx.doi.org/10.17504/protocols.io.bp2l6x6odlqe/v1 | https://www.protocols.io/view/direct-acid-extraction-c8gqztvw | Sigrid Verhelst, Laura Corveleyn | TITLE: Direct acid extraction
AUTHORS: Sigrid Verhelst, Laura Corveleyn
[DESCRIPTION]
Protocol to extract histones from cells using direct acid extraction with the purpose of label-free LC-MS/MS measurement.
[STEPS]
1. Resuspend the pellet in 0,4 N HCl by soft pipetting until no clumps are left in solution (125 µL fo... | ["Resuspend the pellet in 0,4 N HCl by soft pipetting until no clumps are left in solution (125 µL for 1x106cells)", "Incubate 4h in acid on rotator 4°C to promote lysis of nuclei and solubilization of histones.", "Spin for 10 minutes at 4°C, 16000 g.", "Transfer supernatant to new Eppendorfs.", "Add, drop by drop (ve... |
null | null | null | dx.doi.org/10.17504/protocols.io.dbc2iv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<br />(store at 4 degrees Celsius)<br />80% Ethanol<br />4% Formaldehyde<br />5% Acetic Acid<br />1% Glutaraldehyde<br /><br />Measured Instructions<br />Total volume 250 ml FAAG:<br />200 ml 100% Ethanol<br />27.5 ml 37% Formaldehyde<br />12.5 ml Glacial Acetic Acid<br />10 ml ... | [] |
21,899 | Using the Millifluidic Device | 1 | null | https://www.protocols.io/view/using-the-millifluidic-device-zmjf44n | Brandon Tuck | TITLE: Using the Millifluidic Device
AUTHORS: Brandon Tuck
[DESCRIPTION]
Sterile manufacture of indented PDMS base, agar sheet and peripherals for agar perfusion device, along with protocols for operation in sterile conditions
[STEPS]
6. EXPERIMENTAL PROCEDURE - STREAK
Need:
Overnight culture (MADE PREVIOUS DAY)
Ster... | ["EXPERIMENTAL PROCEDURE - STREAK\nNeed:\nOvernight culture (MADE PREVIOUS DAY)\nSterile 250 mL conical flask\nNutrient media\nAssembled device\n60 mL syringe per channel\n25G needle per channel\nDried agar sheet\nRazor blade\nFlat spatula\nDevice holder\nSterile cotton swab\n\nIn a sterile environment, set up a cultur... |
null | null | null | dx.doi.org/10.17504/protocols.io.ewkbfcw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?. | [] |
66,459 | Expression and purification GST-tagged ATG13:ATG101 constructs | 4 | dx.doi.org/10.17504/protocols.io.n92ldzx59v5b/v1 | https://www.protocols.io/view/expression-and-purification-gst-tagged-atg13-atg10-cc53sy8n | Adam Yokom, Xuefeng Ren | TITLE: Expression and purification GST-tagged ATG13:ATG101 constructs
AUTHORS: Adam Yokom, Xuefeng Ren
[DESCRIPTION]
Expression and purification of GST-tagged ATG13/ATG101 constructs
[STEPS]
SECTION: Expression
1. Transfect HEK GNTI cells at concentration of 2 × 106 cells/ml
SECTION: Purification
8. Resuspe... | ["[Expression] Transfect HEK GNTI cells at concentration of 2 × 106 cells/ml", "[Purification] Resuspended pellet in lysis buffer (25 mM HEPES pH 7.5, 200 mM NaCl, 2 mM MgCl2, 1 mM TCEP, 5 mM EDTA, 10% Glycerol) with 1% Triton X-100 and protease inhibitor cocktail (Thermo Scientific, Waltham, MA)", "[Expression] Dilute... |
62,982 | Viaketo Gummies Canada & Via keto Gummies Reviews (Scam Alert) | 1 | dx.doi.org/10.17504/protocols.io.6qpvr6793vmk/v1 | https://www.protocols.io/view/viaketo-gummies-canada-amp-via-keto-gummies-review-b9rer53e | viaketobhbcanada | TITLE: Viaketo Gummies Canada & Via keto Gummies Reviews (Scam Alert)
AUTHORS: viaketobhbcanada
[DESCRIPTION]
This is a sponsored article. The article should not be considered as advice.
[STEPS]
1. Get ViaKeto Gummies in Canada and USA in 2022 and Read Customer Reviews!
The genuine idea of all dietary keto enh... | ["Get ViaKeto Gummies in Canada and USA in 2022 and Read Customer Reviews!\n\nThe genuine idea of all dietary keto enhancements must be known upon use and most frequently the fact of the matter is exceptionally cruel than can be processed and in this, your wellbeing is tested upon. ViaKeto Gummies is the upgraded one t... |
72,918 | CODEX ® Multiplexed Imaging – tissue staining in TMA and whole tissue FFPE sections | 4 | null | https://www.protocols.io/view/codex-multiplexed-imaging-tissue-staining-in-tma-a-cjfwujpe | Anna Martinez Casals, Inna Sitnik, Christian M. Schürch, Mikael Malmqvist, Patrick E Macdonald, Emma Lundberg | TITLE: CODEX ® Multiplexed Imaging – tissue staining in TMA and whole tissue FFPE sections
AUTHORS: Anna Martinez Casals, Inna Sitnik, Christian M. Schürch, Mikael Malmqvist, Patrick E Macdonald, Emma Lundberg
[DESCRIPTION]
CODEX technology allows for highly multiplexed analysis of + 40 proteins on the same section re... | ["[Post staining steps] The day after: remove the coverslip from the humidity chamber, place the coverslip in a well with Staining Buffer (6-well plate) and immerse the coverslip 2-3 times. Incubate for 2 min.", "[Primary antibody staining steps] Using a coverslip forceps, transfer the coverslip into a well (6-well pla... |
80,905 | Field protocol for river water sampling | 1 | null | https://www.protocols.io/view/field-protocol-for-river-water-sampling-cs9hwh36 | Christopher LeBoa, Sneha Shrestha | TITLE: Field protocol for river water sampling
AUTHORS: Christopher LeBoa, Sneha Shrestha
[DESCRIPTION]
This protocol is for the collection of water samples for molecular analysis. It was used for collection of river water samples from Kathmandu for detection of S. Typhi and Paratyphi A
[GUIDELINES]
NOTE: Bags are ... | ["[Bag Preparation for sampling (Two separate bags, one for samples and another for sampling materials Or a bag with separate compartments for samples and the materials)] Wipe the inner part of the sample bag with 0.5% Hypochlorite followed by 70% ethanol.", "[Bag Preparation for sampling (Two separate bags, one for sa... |
103,616 | Profiling the Surfaceome of Meningioma for Immunotherapeutic Target Identification | 0 | dx.doi.org/10.17504/protocols.io.5qpvokqm9l4o/v1 | https://www.protocols.io/view/profiling-the-surfaceome-of-meningioma-for-immunot-dhe833hw | ABDULLAH BIN ZUBAIR, Muhammad Awais Khan, Fatima Hamid, Renee M. Hirte, mlabib, dgandhi | TITLE: Profiling the Surfaceome of Meningioma for Immunotherapeutic Target Identification
AUTHORS: ABDULLAH BIN ZUBAIR, Muhammad Awais Khan, Fatima Hamid, Renee M. Hirte, mlabib, dgandhi
[DESCRIPTION]
This protocol aims to profile the surfaceome of meningioma tumors and matched healthy tissue to identify differentiall... | ["[Subject Terms:] Meningioma, Immunotherapy, Surfaceome profiling, Target identification", "[Keywords:] Meningioma, Immunotherapy, Surfaceome, Proteomics, Mass Spectrometry", "[Introduction] Meningioma is the most common primary brain tumor in adults, characterized by significant treatment challenges and variable recu... |
58,867 | Validating Diversity in DNA Libraries through NGS | 5 | null | https://www.protocols.io/view/validating-diversity-in-dna-libraries-through-ngs-b5qtq5wn | Steffi Davison | TITLE: Validating Diversity in DNA Libraries through NGS
AUTHORS: Steffi Davison
[DESCRIPTION]
DNA libraries are important resources to derive targets to be used for a wide range of applications, from structural and functional studies to intracellular protein interference studies to developing new diagnostics and ... | ["[Complexity inferred by NGS of the library (library sequencing preparation)] The sample of the library, with length 0.5 µL, was prepared for “shotgun” sequencing on a high-throughput Illumina MiSeq sequencer (LBL)", "[Complexity inferred by NGS of the library (library sequencing preparation)] For each sample, 3.75 µL... |
98,528 | Procedure for Seeding Cells on the Disque Platform | 4 | dx.doi.org/10.17504/protocols.io.4r3l24ppjg1y/v2 | https://www.protocols.io/view/procedure-for-seeding-cells-on-the-disque-platform-dcf82trw | Peter Anthony Jones*, Kisuk Yang*, Jeffrey M Karp | TITLE: Procedure for Seeding Cells on the Disque Platform
AUTHORS: Peter Anthony Jones*, Kisuk Yang*, Jeffrey M Karp
[DESCRIPTION]
Advances in treating β cell loss include islet replacement therapies or increasing cell proliferation rate in type 1 and type 2 diabetes. We previously developed a proliferation-inducing p... | ["DP fabrication\n\n1. Disques ( inner diameter) were engraved by laser cutter from thick acrylic sheets. \n\n2. A 1.0μm pore sized-hydrophilic PTFE membrane was attached to the bottom of a Disque using acrylic glue. \n\n3. The reverse side of the membrane was attached to a supporting pedestal engraved by a laser c... |
94,722 | Structural Analysis of 20S CPs and Assembly Intermediates by Electron Cryo-Microscopy | 4 | dx.doi.org/10.17504/protocols.io.x54v9px14g3e/v1 | https://www.protocols.io/view/structural-analysis-of-20s-cps-and-assembly-interm-c8razv2e | Frank Adolf | TITLE: Structural Analysis of 20S CPs and Assembly Intermediates by Electron Cryo-Microscopy
AUTHORS: Frank Adolf
[DESCRIPTION]
This protocol details methods for structural determination by transmission electron cryo-microscopy of 20S CPs and assembly intermediates.
[GUIDELINES]
Please familiarise yourself with the... | ["[Plung freezing of 20S CPs amd 20S CP assembly intermediates] Prepare Vitrobot and grids for plunging\nSet up Vitrobot as follows: blot force = 3, blot time = 3 s sec, humidity 100%, temperature 4 °C\nPlasma clean Quantifoil R1.2/1.3 Cu 200 grids for 45 s sec , just before plunging", "[Plung freezing of 20S CPs amd... |
50,060 | Expression and purification of untagged asynuclein | 1 | dx.doi.org/10.17504/protocols.io.bu5kny4w | https://www.protocols.io/view/expression-and-purification-of-untagged-asynuclein-bu5kny4w | Alain Ndayisaba | TITLE: Expression and purification of untagged asynuclein
AUTHORS: Alain Ndayisaba
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the generation of untagged a-synuclein.</div></div>
[STEPS]
?. [Expression]
Thaw - of BL21(DE3) competent E. coli for ~ or until melted.
20 µl
50... | ["[Expression]\nThaw - of BL21(DE3) competent E. coli for ~ or until melted.\n20 µl\n50 µl\non ice\nIf more than of cells are to be thawed, the remaining aliquot can be frozen using a 100% ethanol and dry ice bath.", "[Expression]\nOnce the cells are completely thawed, add - of - of pET21a-alpha-synuclein and gently... |
null | null | null | dx.doi.org/10.17504/protocols.io.n7mdhk6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<p>This protocol was designed and developed at this laboratory.</p>
<p>The assay specifically targets the 3' UTR region of DENV-1 and is designed as a qualitative screening test for human cases of DENV-1 infection, but not other known DENVs.</p>
</div>
[BEFORE_START]
<div... | [] |
56,622 | RNA Extraction and Quality Assessment targeting SARS-CoV-2 from Wastewater Concentrates using Zymo Environ Water RNA Kit | 4 | dx.doi.org/10.17504/protocols.io.b3inqkde | https://www.protocols.io/view/rna-extraction-and-quality-assessment-targeting-sa-b3inqkde | Tamara Walsky, Padmini Ramachandran, Amanda Windsor, Maria Hoffmann, Chris Grim | TITLE: RNA Extraction and Quality Assessment targeting SARS-CoV-2 from Wastewater Concentrates using Zymo Environ Water RNA Kit
AUTHORS: Tamara Walsky, Padmini Ramachandran, Amanda Windsor, Maria Hoffmann, Chris Grim
[DESCRIPTION]
The Zymo Environ Water RNA kit has been tested and evaluated for the application ... | ["[Sample Homogenization] Add 750 µl of DNA/RNA Shield™ to the concentrated wastewater sample (~200 µl, or to 250 µl liquid) sample to obtain a total of 1 ml of mixture. Mix well by pipetting up and down.", "[Sample Homogenization] Add the 1 ml mixture to a ZR BashingBead™ Lysis Tube.", "[Sample Homogenization] 12000 x... |
55,484 | Targeted Expansion Sequencing Protocols | 2 | dx.doi.org/10.17504/protocols.io.b2e4qbgw | https://www.protocols.io/view/targeted-expansion-sequencing-protocols-b2e4qbgw | Anubhav Sinha, Yi Cui, Shahar Alon, Fei Chen, Asmamaw T. Wassie, Ed Boyden | TITLE: Targeted Expansion Sequencing Protocols
AUTHORS: Anubhav Sinha, Yi Cui, Shahar Alon, Fei Chen, Asmamaw T. Wassie, Ed Boyden
[DESCRIPTION]
This protocol collection accompanies accompanies Expansion Sequencing (ExSeq), covering the four key steps of a targeted Expansion Sequencing (targeted ExSe... | [] |
106,656 | Purification of GST-FAM134C | 0 | dx.doi.org/10.17504/protocols.io.kxygxy8zkl8j/v1 | https://www.protocols.io/view/purification-of-gst-fam134c-dkd84s9w | Elias Adriaenssens | TITLE: Purification of GST-FAM134C
AUTHORS: Elias Adriaenssens
[DESCRIPTION]
This protocol details the purification of GST-FAM134C and its analysis.
[STEPS]
SECTION: Purification procedure
1. To purify GST-FAM134C, fuse the cytosol-exposed domain of FAM134C (250-466aa) to a N-terminal synthesize the gene GST-tag by G... | ["[Purification procedure] To purify GST-FAM134C, fuse the cytosol-exposed domain of FAM134C (250-466aa) to a N-terminal synthesize the gene GST-tag by Genscript and clone into a pGEX-4T1 vector. Plasmid is also available from Addgene.", "[Purification procedure] After the transformation of the pGEX-4T1 vector encoding... |
null | null | null | dx.doi.org/10.17504/protocols.io.f2mbqc6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?. | [] |
68,882 | Liposome preparation | 1 | dx.doi.org/10.17504/protocols.io.6qpvr612ovmk/v1 | https://www.protocols.io/view/liposome-preparation-cfhstj6e | Xinbo Wang, Pietro De Camilli | TITLE: Liposome preparation
AUTHORS: Xinbo Wang, Pietro De Camilli
[DESCRIPTION]
This protocol details methods for the preparation of 100% PS liposome and GC/PS lipid nanotubes used for LRRK2 binding, tubulation assays.
[STEPS]
SECTION: Liposome preparation
1. Dissolve lipid mixtures with chloroform in glass vials in... | ["[Liposome preparation] Dissolve lipid mixtures with chloroform in glass vials in moles percent as follows:\n\nPS liposomes: 99.5% brain PS:0.5% Rhod-PE.\nGC/PS nanotubes: 39.5% Galactosylceramide:60% brian PS:0.5% Cy5-PE.", "[Liposome preparation] Evaporate chloroform under a stream of nitrogen gas to produce a lipid... |
101,123 | Purification of CK2 kinase complex | 0 | dx.doi.org/10.17504/protocols.io.eq2lyww1evx9/v1 | https://www.protocols.io/view/purification-of-ck2-kinase-complex-dezb3f2n | Elias Adriaenssens | TITLE: Purification of CK2 kinase complex
AUTHORS: Elias Adriaenssens
[DESCRIPTION]
This protocol details the purification of CK2 kinase complex.
[STEPS]
SECTION: Purification procedure
1. To purify the CK2 kinase complex, subclone GST-TEV-CK2α together with CK2β in a pFastBac-Dual vector (available from Addgene) and... | ["[Purification procedure] To purify the CK2 kinase complex, subclone GST-TEV-CK2α together with CK2β in a pFastBac-Dual vector (available from Addgene) and GST-TEV-CK2α’ together with CK2β in a pFastBac-Dual vector (available from Addgene) for co-expression in insect cells.", "[Purification procedure] Use the construc... |
50,067 | Transformation of competent E. coli | 4 | null | https://www.protocols.io/view/transformation-of-competent-e-coli-bu5tny6n | yasoo | TITLE: Transformation of competent E. coli
AUTHORS: yasoo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Marburg iGEM 2021 Team's Competent E. coli transformation protocol</div></div>
[STEPS]
?. Briefly thaw chemically competent E. coli cells on ice.
?. Add ∼ of plasmid-DNA to the cells. Alterna... | ["Briefly thaw chemically competent E. coli cells on ice.", "Add ∼ of plasmid-DNA to the cells. Alternatively just use for retransformations and for freshly cloned constructs (Golden Gate reactions).\n100 ng\n[or 2 µl]\n5 µl", "Incubate on ice for (can be skipped for retransformations).", "Heat shock the cells at ... |
null | null | null | dx.doi.org/10.17504/protocols.io.d7f9jm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This is the protocol for QIAgen's QIAprep Spin Miniprep Kit (catalog numbers 27104 or 27106) with their newer version 2.0 spin columns. For more information about the kit, see <a href="https://www.qiagen.com/us/shop/sample-technologies/dna/dna-preparation/qiaprep-spin-miniprep-k... | [] |
81,669 | Tissue Sectioning Guidelines (FFPE) - CODEX/PhenoCycler | 4 | null | https://www.protocols.io/view/tissue-sectioning-guidelines-ffpe-codex-phenocycle-ctzdwp26 | Santhosh Sivajothi | TITLE: Tissue Sectioning Guidelines (FFPE) - CODEX/PhenoCycler
AUTHORS: Santhosh Sivajothi
[DESCRIPTION]
The purpose of this SOP is to provide general guidelines for preparing FFPE tissue sections suitable for PhenoCycler/CODEX
[STEPS]
SECTION: Tissue Sectioning Guidelines for CODEX/PhenoCycler
1. Prepare the microto... | ["[Tissue Sectioning Guidelines for CODEX/PhenoCycler] Prepare the microtome for sectioning. Section the tissue at a thickness of 5 μm.", "[Tissue Sectioning Guidelines for CODEX/PhenoCycler] For best results, the tissue should be completely adhered to the slide with minimal tears or folds.", "[Tissue Sectioning Guidel... |
74,576 | Procedure of total sugar content, proline, chlorophyll a, b, total, SOD, POD, H2O2 and APX | 4 | dx.doi.org/10.17504/protocols.io.5jyl8je1dg2w/v1 | https://www.protocols.io/view/procedure-of-total-sugar-content-proline-chloroph-ck3quymw | Syarifah Aini Pasaribu, Mohammad Basyuni | TITLE: Procedure of total sugar content, proline, chlorophyll a, b, total, SOD, POD, H2O2 and APX
AUTHORS: Syarifah Aini Pasaribu, Mohammad Basyuni
[DESCRIPTION]
Analysis of physiological characteristics was carried out at the Physiology and 77 Protection Laboratory of the Unit Research Sungei Putih,... | ["Total sugar content \n\nReactor: \n1. Anthrone reagent 0.1 g anthrone added to 100 ml sulfate solution \n2. Sulfuric acid solution: sulfuric acid: distilled water (100:29)\n3. TCA 2.5%: 2.5 gr TCA dissolved in 100 ml of distilled water \n4. Standard 2 mM sucrose:\nStock I (40 mM): 69 mg sucrose dissolved in 5 ml TCA ... |
89,664 | Barnes Maze | 1 | dx.doi.org/10.17504/protocols.io.5qpvo3qdxv4o/v1 | https://www.protocols.io/view/barnes-maze-c3s8ynhw | Jhodi Webster | TITLE: Barnes Maze
AUTHORS: Jhodi Webster
[DESCRIPTION]
This protocol provides a detailed, step-by-step guide for conducting the Barnes maze test, a well-established behavioral assay for assessing spatial learning and memory in mice and rats. The maze utilizes a rodent's innate aversion to open, brightly lit spaces an... | ["[Setup] Have a clear open space in the middle of your behaviour room to work with.", "[Setup] Tear napkin pieces into small squares to prepare for escape box covering", "[Setup] Organize images (cross, triangle and circle) on the walls adjacent to maze apparatus. Ensure they are all at the same x and y plane.", "[Set... |
null | null | null | dx.doi.org/10.17504/protocols.io.fyqbpvw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This procedure is optimized for the isolation of 10<sup>6</sup> cells per test. If working with fewer than 10<sup>6</sup><br />cells, keep volumes as indicated for 10<sup>6</sup> cells. For best results, optimize the conditions to your specific cell number and<br />tissue. Pr... | [] |
41,713 | Preparing biological samples for metabarcoding | 4 | dx.doi.org/10.17504/protocols.io.bkyrkxv6 | https://www.protocols.io/view/preparing-biological-samples-for-metabarcoding-bkyrkxv6 | Tim Regan | TITLE: Preparing biological samples for metabarcoding
AUTHORS: Tim Regan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the preparation of biological samples (specifically from a marine environment e.g. hatchery or RAS unit) for amplicon sequencing. Starting with a biologica... | ["[Bead beating]\nStarting with biological sample (filter, swab, water, biofilm, tissue etc.) stored in Qiagen Buffer ATL (or similar), transfer up to to Matrix A bead tube.\n1 mL", "[Bead beating]\nPerform bead beating in a disruptor at at 5.0 M/s (speed) for x2 (ensure tube looks homogenous).", "[Enzymatic digestio... |
84,319 | Total particulate carbohydrate from microalgae | 4 | dx.doi.org/10.17504/protocols.io.yxmvmk24ng3p/v2 | https://www.protocols.io/view/total-particulate-carbohydrate-from-microalgae-cwj7xcrn | Ying-Yu Hu, Zoe V. Finkel | TITLE: Total particulate carbohydrate from microalgae
AUTHORS: Ying-Yu Hu, Zoe V. Finkel
[DESCRIPTION]
Here we describe a protocol to estimate the total particulate carbohydrate from microalgae. Carbohydrate samples are initially vortexed in 9 M H2SO4 for 15 s. The solution is diluted for a final H2SO4 molarity of 1.6... | ["[Day 1 - Samples] Considering the working hours from 9 am to 4 pm, suggested sample number is:\n# of blank + # of samples = 24", "[Day 1- Hydrolysis] Transfer 18 M H2SO4 into a precombusted glassware (such as, scintillation vial, beaker... etc)", "[Day 1- Hydrolysis] Add 4.5 mL MilliQ, tightly cap the centrifuge tube... |
41,820 | GNRI's COVID-19 Antigen Lateral Flow Test Device Use Steps | 4 | dx.doi.org/10.17504/protocols.io.bk34kyqw | https://www.protocols.io/view/gnri-x27-s-covid-19-antigen-lateral-flow-test-devi-bk34kyqw | Dr Georgios Gerardos | TITLE: GNRI's COVID-19 Antigen Lateral Flow Test Device Use Steps
AUTHORS: Dr Georgios Gerardos
[STEPS]
?. [Please follow all steps]
Obtain a clean container (glass or cup) and produce a saliva sample in the container.
?. [Please follow all steps]
Remove a new COVID-19 Antigen Lateral Flow Test Device from its foil pa... | ["[Please follow all steps]\nObtain a clean container (glass or cup) and produce a saliva sample in the container.", "[Please follow all steps]\nRemove a new COVID-19 Antigen Lateral Flow Test Device from its foil packaging.", "[Please follow all steps]\nPlace the test strip in the container and leave for 10min.", "[Pl... |
null | null | null | dx.doi.org/10.17504/protocols.io.ivece3e | null | null | TITLE: No Title
AUTHORS:
[BEFORE_START]
<p>The protocol remains unmodified from original kit protocol, with the exception of steps 6, 7 and 8.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
58,777 | Setting up a new algal chemostat protocol | 3 | null | https://www.protocols.io/view/setting-up-a-new-algal-chemostat-protocol-b5mzq476 | Rebecca Bilich, Meghan Duffy | TITLE: Setting up a new algal chemostat protocol
AUTHORS: Rebecca Bilich, Meghan Duffy
[DESCRIPTION]
This is a protocol used to set up a new algal chemostat.
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.e2hbgb6 | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p>- Use with Ultra Streptavidin Detection Kit (SIG-32250) or (SIG-32248)</p>
<p> </p>
<p>- Positive control: Normal human skin</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
60,230 | iTracer Plasmid Prep | 4 | null | https://www.protocols.io/view/itracer-plasmid-prep-b63ergje | Ashley Maynard, Hsiu-Chuan Lin | TITLE: iTracer Plasmid Prep
AUTHORS: Ashley Maynard, Hsiu-Chuan Lin
[DESCRIPTION]
Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enable highly resolved descriptions of cell states within these systems; however, approaches are needed... | ["[Order iTracer plasmids] Please order iTracer from the European Plasmid Repository (https://www.plasmids.eu/).\n\npSBbi-iTracer-G\npSBbi-iTracer-R", "[Order barcodes] To ensure the greatest barcode diversity please order the barcode components below:\n\nbarcode template: gacgagctgtacaagtgatccgWNNNNNNNNNWcacccagctttct... |
53,210 | nanopore nCoV-2019 sequencing protocol (RAPID barcoding, 1200bp amplicon, combined RT-PCR) | 1 | dx.doi.org/10.17504/protocols.io.bx72prqe | https://www.protocols.io/view/nanopore-ncov-2019-sequencing-protocol-rapid-barco-bx72prqe | Anton Pembaur, Erwan Sallard, Patrick Weil, Jennifer Ortelt, Parviz Ahmad-Nejad, Jan Postberg | TITLE: nanopore nCoV-2019 sequencing protocol (RAPID barcoding, 1200bp amplicon, combined RT-PCR)
AUTHORS: Anton Pembaur, Erwan Sallard, Patrick Weil, Jennifer Ortelt, Parviz Ahmad-Nejad, Jan Postberg
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">We established a protocol for fast, cost efficie... | ["[Sample selection, RNA isolation]\nFor whole genome sequencing using combined RT-PCR, samples with a Ct Isolate RNA using any suitable protocol. We tried magnetic Bead based (Nimbus/TanBead), spin collum based (QIAamp Viral RNA, Qiagen) and trizol/chloroforme extraction, with no significant differences.", "[Multiplex... |
79,841 | Blue native-PAGE of protein complexes in plant cells | 1 | dx.doi.org/10.17504/protocols.io.3byl4jyd8lo5/v1 | https://www.protocols.io/view/blue-native-page-of-protein-complexes-in-plant-cel-cr79v9r6 | Hee-Kyung Ahn, Jonathan Jones | TITLE: Blue native-PAGE of protein complexes in plant cells
AUTHORS: Hee-Kyung Ahn, Jonathan Jones
[DESCRIPTION]
Many protein complexes exist in cells, and these protein complexes are vital for cellular processes. However, much of our current analyses of protein-protein interaction mainly focus on binary interactions.... | ["[Sample preparation] The following cases describe sample harvest methods using trasiently-infiltrated Nicotiana benthamiana (4-5 weeks old), Arabidopsis seedlings (~2 weeks old), or Arabidopsis leaves (4-5 weeks old.)", "[Protein extraction] Grind samples in 2 ml tubes using a tissue homogenizer (GenoGrinder), at 120... |
72,947 | Application of PHYTO-PAM-II (Compact Version) For Running Rapid light curves on Cyanobacterial samples | 1 | dx.doi.org/10.17504/protocols.io.eq2ly7jqelx9/v1 | https://www.protocols.io/view/application-of-phyto-pam-ii-compact-version-for-ru-cjgtujwn | Gwen Stark, Emily E. Chase, Steven W Wilhelm | TITLE: Application of PHYTO-PAM-II (Compact Version) For Running Rapid light curves on Cyanobacterial samples
AUTHORS: Gwen Stark, Emily E. Chase, Steven W Wilhelm
[DESCRIPTION]
A protocol used to acquire photosynthetic efficiency (Fv/Fm) and quantum yield of photosystem II (Y(II)) of Microcystis aeruginosa cultures u... | ["[SAMPLE PREPARATION] For each biological replicate of your cyanobacteria culture, 3mL of culture will need to be transferred into a quartz cuvette that comes with the Phyto-Pam. It is best to leave your cultures in their treatment conditions until you are ready to run your rapid light curves. Minimize exposure time a... |
null | null | null | dx.doi.org/10.17504/protocols.io.f7gbrjw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>CZV8-top agar</strong> </p>
<p> <strong><u>100 mL</u></strong> per transformation <strong><u>200 mL</u></strong></p>
<p>Czapek-Dox liquid 4.54 g ... | [] |
46,333 | protocols in WDR81 paper | 4 | dx.doi.org/10.17504/protocols.io.brg5m3y6 | https://www.protocols.io/view/protocols-in-wdr81-paper-brg5m3y6 | Yang Li | TITLE: protocols in WDR81 paper
AUTHORS: Yang Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Experimental Procedures</span></div><div class = "text-block"><span style = "font-weight:bold;">Cell culture, reagents, and transfection</span></div><div class = "text-b... | [] |
83,810 | Incell Scanning Protocol | 5 | dx.doi.org/10.17504/protocols.io.6qpvr3erovmk/v1 | https://www.protocols.click/view/incell-scanning-protocol-cv4aw8se | jwaligor | TITLE: Incell Scanning Protocol
AUTHORS: jwaligor
[DESCRIPTION]
Incell Scanning Protocol
[STEPS]
SECTION: Live Cell Chamber Set Up (if applicable)
1. If your experiment, start set up 15-30 minutes prior to scanning.
SECTION: Live Cell Chamber Set Up (if applicable)
2. Attach live cell chamber plate cover and CO2 lin... | ["[Live Cell Chamber Set Up (if applicable)] If your experiment, start set up 15-30 minutes prior to scanning.", "[Live Cell Chamber Set Up (if applicable)] Attach live cell chamber plate cover and CO2 line to Incell plate area.", "[Live Cell Chamber Set Up (if applicable)] Open CO2 and compressed valves.", "[Live Cell... |
99,383 | WATER PRODUCTION FOR AWARE (Virus) | 0 | dx.doi.org/10.17504/protocols.io.n92ld8z2ov5b/v1 | https://www.protocols.io/view/water-production-for-aware-virus-ddax22fn | Celia Manaia | TITLE: WATER PRODUCTION FOR AWARE (Virus)
AUTHORS: Celia Manaia
[DESCRIPTION]
The protocol summarises the procedures used for analytical control. The protocol describes the
Standard Operating Procedure (SOP) for the optimization of advanced tertiary treatment of water, based on a comprehensive quality and risk assessm... | ["[Virus:] The water production for AWARE main activities includes three stages – disinfection by ultraviolet C radiation (UVC), storage for720 min-1440 min(according to water load and season) and ozonation. The water quality is monitored at these three stages, for the parameters indicated in Figure 1 below.", "[Virus:... |
30,179 | UF / HuBMAP - H&E Staining Process | null | dx.doi.org/10.17504/protocols.io.9qbh5sn | null | Franchesca Farris, Marda Jorgensen, Leigh Propper | TITLE: UF / HuBMAP - H&E Staining Process
AUTHORS: Franchesca Farris, Marda Jorgensen, Leigh Propper
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol will detail our method of utilizing the H&E stain as a nonspecific entity to achieve optimal results in our staining process for our res... | ["Ensure your staining line or staining set up has sufficient reagents and is correctly set up for use.", "Immerse slide holder in the first xylene staining pot for 5 minutes.", "Remove slide holder from xylene and transfer to the first staining pot of 100% EtOH for 5 minutes.\nBe sure to blot excess xylene before goin... |
10,192 | 16S Bacteria 338F-516P-805R BSA | 1 | dx.doi.org/10.17504/protocols.io.m7qc9mw | https://www.protocols.io/view/16s-bacteria-338f-516p-805r-bsa-m7qc9mw | Roey Angel, Eva Petrova, Ana Lara | TITLE: 16S Bacteria 338F-516P-805R BSA
AUTHORS: Roey Angel, Eva Petrova, Ana Lara
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Universal 16S rRNA BActeria 338F-516P-805R</div><div class = "text-block"><span>BAC338F ACT CCT ACG GGA GGC AG , target </span><span style = "font-style:italic;">E.c... | ["[QPCR mixture]\nABCD1ReagentFinal conc.1 tube (20μl)plate (20μl x 100)2PCR H2O 4.64603iQTM Supermix1x1010004MgCl2 (25mM)4.0 mM0.8*80*5BSA (20μg/μl)0.2 μg/μl0.2206338F (10μM)0.5 μM1.01007805R (10μM) 0.5 μM1.01008516P (10μM)†0.2 μM0.4409Template 22 x 100* Buffer contains MgCl2 at final conc. of 3.0 mM\n... |
69,443 | Methods for Clinical Chart Abstraction: Real-World ALK Inhibitor Treatment Patterns and Reasons for Discontinuation Study | 1 | dx.doi.org/10.17504/protocols.io.4r3l27my3g1y/v1 | https://www.protocols.io/view/methods-for-clinical-chart-abstraction-real-world-cf3btqin | Shadera Slatter, Michelle Wang, Chia-Wei Lin, Jesse Sussell, Sarika Ogale, Debajyoti Datta, Atul Butte, Lyudmila Bazhenova, Vivek A Rudrapatna | TITLE: Methods for Clinical Chart Abstraction: Real-World ALK Inhibitor Treatment Patterns and Reasons for Discontinuation Study
AUTHORS: Shadera Slatter, Michelle Wang, Chia-Wei Lin, Jesse Sussell, Sarika Ogale, Debajyoti Datta, Atul Butte, Lyudmila Bazhenova, Vivek A Rudrapatna
[DESCRIPTION]
This protocol details th... | ["[Demographics] Date of Birth – Date: Defined as the date of subject’s birth.", "[Demographics] Sex – Binary: Male/Female- Defined as the sex assigned at birth.", "[Demographics] Insurance – Nominal: Defined as the insurance assigned to the subject in their electronic health record. If no insurance is recorded in the ... |
50,746 | Protocol G2B study | 3 | dx.doi.org/10.17504/protocols.io.bvs2n6ge | https://www.protocols.io/view/protocol-g2b-study-bvs2n6ge | Sophie Leclercq, Camille Amadieu, Audrey M Neyrinck, Philippe de Timary, Nathalie M Delzenne, Peter Stärkel | TITLE: Protocol G2B study
AUTHORS: Sophie Leclercq, Camille Amadieu, Audrey M Neyrinck, Philippe de Timary, Nathalie M Delzenne, Peter Stärkel
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Background:The gut microbiota is a key player in the regulation of metabolism, immunity and brain functio... | [] |
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