id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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40,868 | Severity Grading Table (Appendix B of Phase 3 study of Vaccine Candidate for COVID-19) | 1 | dx.doi.org/10.17504/protocols.io.bj6ckraw | https://www.protocols.io/view/severity-grading-table-appendix-b-of-phase-3-study-bj6ckraw | Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores | TITLE: Severity Grading Table (Appendix B of Phase 3 study of Vaccine Candidate for COVID-19)
AUTHORS: Chris Ockenhouse, Chris Gast, Renee Holt, Jorge Flores
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is Appendix B of "Phase 3 randomized, double-blinded, placebo-controlled trial to e... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.ri6d4he | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Várias descobertas são publicadas mensalmente com potencial de aperfeiçoar a técnica de analgesia de parto.</p>
<p>A intenção deste protocolo é registrar sistematicamente as mudanças que adotei.</p>
[STEPS]
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63,598 | Static glucagon secretion analysis of isolated islets | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy264lx1/v1 | https://www.protocols.io/view/static-glucagon-secretion-analysis-of-isolated-isl-cacnsave | Julien Ghislain, Vincent Poitout, Caroline CT Tremblay, Marine Croze | TITLE: Static glucagon secretion analysis of isolated islets
AUTHORS: Julien Ghislain, Vincent Poitout, Caroline CT Tremblay, Marine Croze
[DESCRIPTION]
This protocol describes the steps to measure glucagon secretion in a static, 1-hour assay from isolated pancreatic islets. It is suitable for islets isolated from ... | ["[Preparation of KRB solution and plates] Prepare KRB stocks, glucose and L-arginine solutions\nKRB stock I: weigh 27.7 g NaCl bring to 1 L with milliQ water\nKRB stock II: weigh 1.494 g CaCl2•2H2O and bring to 1 L with milliQ water\nKRB stock III: weigh 0.648 g KH2PO4 and bring to 1 L with milliQ water\nKRB stock IV:... |
94,923 | DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing | 1 | dx.doi.org/10.17504/protocols.io.eq2lyjyrplx9/v1 | https://www.protocols.io/view/dna-extraction-protocol-for-saliva-samples-obtaine-c8xjzxkn | Victoria Meslier, Aymeric David, christian.morabito, mathieu.almeida | TITLE: DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing
AUTHORS: Victoria Meslier, Aymeric David, christian.morabito, mathieu.almeida
[DESCRIPTION]
This protocol describes the procedure for DNA extraction of sa... | ["[Salivary Collection Using OMNIgene ORAL OM 501 Device] Visit the protocols.io for saliva sampling : https://dx.doi.org/10.17504/protocols.io.n92ldm8qol5b/v1", "[Sample pre-processing] Heat the whole OMNIgene Oral OM 501 tubes into an incubator at 50 °C for 2 h.", "[DNA extraction QIAGEN QIAamp Power Fecal Pro] Avail... |
22,953 | Pig-Stellate Stimulation Protocol | null | dx.doi.org/10.17504/protocols.io.2nhgdb6 | null | Jeffrey Ardell, Marmar Vaseghi, Olujimi Ajijola | TITLE: Pig-Stellate Stimulation Protocol
AUTHORS: Jeffrey Ardell, Marmar Vaseghi, Olujimi Ajijola
[STEPS]
?. Animal: Yorkshire Pig
?. Surgical Prep: Sedation Telazol (8mg/kg)Anesthesia isoflurane (1-2% inhalation)concomitant with intermittent boluses of fentanyl (1–3 μg/kg iv)
?. Following completion of the surgery, a... | ["Animal: Yorkshire Pig", "Surgical Prep: Sedation Telazol (8mg/kg)Anesthesia isoflurane (1-2% inhalation)concomitant with intermittent boluses of fentanyl (1–3 μg/kg iv)", "Following completion of the surgery, anesthesia was changed to α-chloralose (50 mg/kg intravenous bolus administration followed by continuous infu... |
76,738 | Cryosectioning fresh frozen tissues for multimodal imaging | 1 | dx.doi.org/10.17504/protocols.io.e6nvwjmjzlmk/v1 | https://www.protocols.io/view/cryosectioning-fresh-frozen-tissues-for-multimodal-cn7avhie | Presha Rajbhandari, Taruna Neelakantan, Brent R Stockwell | TITLE: Cryosectioning fresh frozen tissues for multimodal imaging
AUTHORS: Presha Rajbhandari, Taruna Neelakantan, Brent R Stockwell
[DESCRIPTION]
This protocol describes sectioning for fresh frozen tissues for multimodal imaging including Hematoxylin & Eosin staining, DESI imaging mass spectrometry, SIMS imaging mas... | ["Place the tissue block inside the cryostat at -20°C for 30 mins for equilibration. Also, place the slides and substrates inside the cryostat for cooling.", "Mount the tissue block on the chuck using water (no OCT) and adjust the angle of the chuck as needed.", "Trim the tissue block at 30-50µm thickness to obtain a f... |
20,954 | UC Davis - Insulin signaling pathway | null | dx.doi.org/10.17504/protocols.io.yp2fvqe | null | Fawaz G. Haj | TITLE: UC Davis - Insulin signaling pathway
AUTHORS: Fawaz G. Haj
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">
This test is designated to determine defects in the insulin signaling pathway, through evaluation of the... | ["Fast mice for 16 hours by taking away food the day before (3:00pm)", "The following day, give the mouse an intraperitoneal injection of insulin (10 U/kg) with a 27 G needle.", "Use cervical dislocation to euthanize mice.", "Collect maximum blood from portal vein and isolate plasma according to standard protocols or a... |
null | null | null | dx.doi.org/10.17504/protocols.io.jwycpfw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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77,080 | Estimate phospholipids from microalgae | 4 | dx.doi.org/10.17504/protocols.io.q26g74r19gwz/v2 | https://www.protocols.io/view/estimate-phospholipids-from-microalgae-cphyvj7w | Ying-Yu Hu, Zoe V. Finkel | TITLE: Estimate phospholipids from microalgae
AUTHORS: Ying-Yu Hu, Zoe V. Finkel
[DESCRIPTION]
Here we describe a protocol to estimate phospholipids from microalgae.
After extracting and measuring the total lipids from microalgae, the remaining lipid extract is dried using a nitrogen flow, followed by drying with ma... | ["[Prepare phospholipids sample] Dry remaining organic phase extract of total lipids at 37 °C under a stream of N2 gas (<2 psi)", "[High temperature dry combustion] Use diamond pen to engrave the sample vials with numbers. Log number and sample code.", "[High temperature dry combustion] 0.17 M MgSO4 reagent:\n\nDissolv... |
87,493 | A method for the temperature-controlled extraction of DNA from ancient bones | 1 | dx.doi.org/10.17504/protocols.io.rm7vz3pb2gx1/v2 | https://www.protocols.io/view/a-method-for-the-temperature-controlled-extraction-czpdx5i6 | Elena Essel, Matthias Meyer, Petra Korlevic | TITLE: A method for the temperature-controlled extraction of DNA from ancient bones
AUTHORS: Elena Essel, Matthias Meyer, Petra Korlevic
[DESCRIPTION]
We here provide a protocol for the decontamination of ancient bones and teeth that is based on a temperature-controlled, sequential release of DNA. DNA can be extracte... | ["[Buffer preparation]", "[Buffer preparation] Sodium-phosphate buffer (0.5 M sodium phosphate, pH 7.0, 0.1 % Tween 20) is prepared by combining the following reagents:\n\n49.5 mL \n50 µL", "[Buffer preparation] Tris-Tween wash buffer (10 mM Tris-HCl, pH 8.0, 0.1% Tween-20) is prepared by combining the following rea... |
86,344 | Stereotaxic Surgery | 1 | dx.doi.org/10.17504/protocols.io.kqdg3x9j1g25/v1 | https://www.protocols.io/view/stereotaxic-surgery-cyjgxujw | Beatriz E Nielsen | TITLE: Stereotaxic Surgery
AUTHORS: Beatriz E Nielsen
[DESCRIPTION]
This protocol describes the steps for performing mouse stereotaxic surgery. It is applicable to intracranial injections of viruses and drugs.
[STEPS]
SECTION: Before Surgery
1. Autoclave instruments (forceps, clamps, and scalpel holders; max 5 mice ... | ["[Before Surgery] Autoclave instruments (forceps, clamps, and scalpel holders; max 5 mice per tool pack) and cotton swabs.", "[Before Surgery] Pull injector glass micropipettes.", "[Before Surgery] Gather supplies needed for surgery (viruses, drugs, anesthetics, analgesics).", "[Surgery Setup] Disinfect surgical stati... |
52,127 | Generation of stable cell lines using Lentiviral vectors | 4 | dx.doi.org/10.17504/protocols.io.bw57pg9n | https://www.protocols.io/view/generation-of-stable-cell-lines-using-lentiviral-v-bw57pg9n | maxime.smits , Chris Van den Haute, Veerle Baekelandt | TITLE: Generation of stable cell lines using Lentiviral vectors
AUTHORS: maxime.smits , Chris Van den Haute, Veerle Baekelandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Generation of a stable cell line refers to</span><span style = "font-weight:bold;">the process of developing homogenou... | ["[Day 0]\nSEEDING CELLS", "[Day 0]\nCount the cells with a cell counter (keep the range of correct measurement of the device into account).", "[Day 0]\nBased on the cell count, dilute the cells to seed a suitable amount in a 96-well plate. For example, you want to put 20.000 cells in 200 µL medium in each well of a 9... |
85,783 | Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 2 (Craniotomy) | 1 | dx.doi.org/10.17504/protocols.io.n92ldmz48l5b/v1 | https://www.protocols.io/view/essential-surgeries-for-the-electrophysiological-r-cxzxxp7n | Daisuke Kase, Witold J Lipski, Devin R Harsch, Robert S Turner | TITLE: Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 2 (Craniotomy)
AUTHORS: Daisuke Kase, Witold J Lipski, Devin R Harsch, Robert S Turner
[DESCRIPTION]
Outlined below are the steps to complete a craniotomy in an existing recording chamber placed on a cranial impla... | ["[Preparation in the prep room on the day of surgery] Sedate the animal.", "[Preparation in the prep room on the day of surgery] Intubate the animal, start the gas anesthesia, and bring it to the surgical suite.", "[Procedure in the surgical suite] Secure the animal’s head on the stereotaxic frame.", "[Procedure in th... |
null | null | null | dx.doi.org/10.17504/protocols.io.uibeuan | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol describes how to deliver ribonucleoprotein (RNP) complexes that consist of purified Cas9 nuclease duplexed with chemically modified synthetic single guide RNA (sgRNA) to standard immortalized cell lines (adherent or suspension). Although optimized for HEK293 (hu... | ["[Pre-Lipofection - Seed cells] {\"blocks\":[{\"key\":\"5njva\",\"text\":\"Seed cells and incubate in 37\\u00b0C\\/5% CO2 incubator overnight so that they are 30-70% confluent on the day of transfection (Day 2).\\n \\n \\n \",\"type\":\"unstyled\",\"depth\":0,\"inlineStyleRanges\":[{\"offset\":37,\"length\":1,\"style... |
97,061 | Correlated light-electron microscopy (CLEM) and cryo-focused ion beam (FIB) milling | 0 | dx.doi.org/10.17504/protocols.io.dm6gpzexjlzp/v1 | https://www.protocols.io/view/correlated-light-electron-microscopy-clem-and-cryo-da2d2ga6 | Dorothy zhao | TITLE: Correlated light-electron microscopy (CLEM) and cryo-focused ion beam (FIB) milling
AUTHORS: Dorothy zhao
[DESCRIPTION]
Aggregation of proteins containing expanded polyglutamine (polyQ) repeats is the cytopathologic hallmark of a group of dominantly inherited neurodegenerative diseases, including Huntington's d... | ["[CLEM] Vitrified sample on autogrids were loaded onto a cryo-confocal LM set up (Leica SP8) equipped with a 50X/0.9 NA objective (Leica Objective), metal halide light source (EL6000), air-cooled detector (DFC900GT), a cryo-stage (-195 °C), and two HyD detectors.", "[CLEM] The sample was kept in liquid nitrogen vapor,... |
null | null | null | dx.doi.org/10.17504/protocols.io.syheft6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol demonstrates how to convert raw long reads produced using a strand-specific sequencing protocol (e.g. ONT's strand-switching protocol) into strand-specific mapped reads.</p>
<p> </p>
<p>The general ides is to use LAST to identify the adapter orientation relative... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.c84zyv | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For use in <a href="https://www.protocols.io/view/Wet-mount-Method-for-Enumeration-of-Aquatic-Viruse-c8pzvm" target="_blank">Wet-mount Method for Enumeration of Aquatic Viruses</a>
[STEPS]
?. | [] |
28,856 | Determination of Podocyte Number and Density in Rodent Glomeruli | null | dx.doi.org/10.17504/protocols.io.8eyhtfw | null | Frank Brosius | TITLE: Determination of Podocyte Number and Density in Rodent Glomeruli
AUTHORS: Frank Brosius
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This protocol describes a validated protocol used by some DiaComp members t... | ["Perfusion fix mouse or rat kidneys with 2% paraformaldehyde or PLP. Embed in paraffin. Cut 3 and 9 um thick sections longitudinally through each kidney (See Vascular Perfusion Protocol). It is important to initially establish the actual section thickness generated by each cryotome since this can vary substantially.",... |
67,016 | Immunofluorescence assay (IFA) | 1 | dx.doi.org/10.17504/protocols.io.5qpvobz99l4o/v1 | https://www.protocols.io/view/immunofluorescence-assay-ifa-cdpgs5jw | Thanh Ngoc Nguyen | TITLE: Immunofluorescence assay (IFA)
AUTHORS: Thanh Ngoc Nguyen
[DESCRIPTION]
This protocol details the procedure of immunofluorescence assay (IFA).
[STEPS]
SECTION: Procedures
1. Seed the HeLa cells onto HistoGrip (ThermoFisher) coated coverslips in growth media in 6 well plates (DMEM supplemented with 10 % (v/v) F... | ["[Procedures] Seed the HeLa cells onto HistoGrip (ThermoFisher) coated coverslips in growth media in 6 well plates (DMEM supplemented with 10 % (v/v) FBS, 1 % Penicillin-Streptomycin, 25 millimolar (mM) HEPES, GlutaMAX (Life Technologies) and non-essential amino acids (Life Technologies)).", "[Procedures] Aspirate off... |
100,405 | Metabarcoding Fecal Swabs or Stomach Contents for Fish and Crustaceans using 2-PCR protocol and Illumina MiSeq | 4 | dx.doi.org/10.17504/protocols.io.ewov1qxokgr2/v3 | https://www.protocols.io/view/metabarcoding-fecal-swabs-or-stomach-contents-for-deav3ae6 | Eldridge Wisely | TITLE: Metabarcoding Fecal Swabs or Stomach Contents for Fish and Crustaceans using 2-PCR protocol and Illumina MiSeq
AUTHORS: Eldridge Wisely
[DESCRIPTION]
This protocol describes a method to metabarcode a 170bp region of the mitochondrial16S rRNA gene of crustaceans and a 163-185bp region of the mitochondrial 12S rR... | ["[Prepare Primers] Order metabarcoding primers with diversity spacers and Illumina overhang sequences (Illumina, 2013): (Miya et al., 2015): \nTCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGTCGGTAAAACTCGTGCCAGC\n \n (Miya et al., 2015): GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNNCATAGTGGGGTATCTAATCCCAGTTTG\n\n Berry et al., 201... |
28,861 | Induce Stroke via Hypoxia-Ischemia | null | dx.doi.org/10.17504/protocols.io.8e5htg6 | null | Ian Simpson | TITLE: Induce Stroke via Hypoxia-Ischemia
AUTHORS: Ian Simpson
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This document describes the procedure for inducing a stroke in diabetic RCS10 mice, their non-diabetic cont... | ["Prior to H/I\nAll instrumentation and disposables utilized for the H/I procedure and/or recovery are autoclaved. MB10 disinfectant is used when instruments cannot be autoclaved.", "Day of H/I\nOn the morning of experimentation, cages are wiped with MB10 and placed in a flow hood and the solid lid is replaced with an ... |
102,788 | Water holding capacity by mass loss | 0 | null | https://www.protocols.io/view/water-holding-capacity-by-mass-loss-dgmc3u2w | Jennifer Moore | TITLE: Water holding capacity by mass loss
AUTHORS: Jennifer Moore
[DESCRIPTION]
Modified protocol based on that published by Nelson et al., 2024, 55(8):1190-1204.
[STEPS]
SECTION: Set-up
1. Fold a Whatman #1 filter paper into quadrants to make a cone shape.
Place into a conical funnel.
Pre-saturate filter with DI wa... | ["[Set-up] Fold a Whatman #1 filter paper into quadrants to make a cone shape.\nPlace into a conical funnel.\nPre-saturate filter with DI water by dispensing water around the edges of the filter paper and allow excess to pass through.", "[Weighing and Drying] Weigh soil plus wet filter paper and record mass to 0.000g. ... |
63,850 | Crasher Keto Gummies: Best For Weight Loss| It Trended Buy Now | 3 | dx.doi.org/10.17504/protocols.io.rm7vzy2p5lx1/v1 | https://www.protocols.io/view/crasher-keto-gummies-best-for-weight-loss-it-trend-cakiscue | DtieSmuh | TITLE: Crasher Keto Gummies: Best For Weight Loss| It Trended Buy Now
AUTHORS: DtieSmuh
[DESCRIPTION]
<!--td {border: 1px solid #ccc;}br {mso-data-placement:same-cell;}-->crasher keto gummies help you increase your energy, and strength, to burn fat fast & get a slim figure *buy now on official site*
[STEPS] | [] |
53,720 | Salting-out Protocol for Extracting HMW Genomic DNA from frozen octocorals | 4 | dx.doi.org/10.17504/protocols.io.bypypvpw | https://www.protocols.io/view/salting-out-protocol-for-extracting-hmw-genomic-dn-bypypvpw | Santiago Herrera | TITLE: Salting-out Protocol for Extracting HMW Genomic DNA from frozen octocorals
AUTHORS: Santiago Herrera
[DESCRIPTION]
Salting-out protocol for extracting high-molecular weight (HMW) genomic DNA from frozen octocorals for genomic methods. Suitable for genome sequencing, RAD-seq and other methods that require HMW DN... | ["[Preparation] Make sure that there are enough Materials and Supplies available and at the right temperature conditions.", "[Preparation] Make sure that the Ultracentrifuge, Heat Block and IncuShaker are set up in the fume hood.", "[Preparation] Place tube rack in IncuShaker.", "[Preparation] Turn on the Heat Block an... |
43,571 | SOP16v1_TGD_Qubit_dsDNA_BR_Assays_for_DNAQuantification | 4 | null | https://www.protocols.io/view/sop16v1-tgd-qubit-dsdna-br-assays-for-dnaquantific-bnstmeen | Varsha Rajesh | TITLE: SOP16v1_TGD_Qubit_dsDNA_BR_Assays_for_DNAQuantification
AUTHORS: Varsha Rajesh
[DESCRIPTION]
Guidelines
Bench work should be completed on the pre-PCR/DNA bench.
Blue DNA-only labeled materials should be used for this protocol to avoid cross-contamination.
Lab attire: gloves, lab coat, and safety glasses/goggl... | ["Prepare the correct amount of mastermix of Qubit reagent in buffer for your samples and standards. \n\nNote: A calibration is required for every usage of the Qubit, so always include standards in the calculations. \nReagent and buffer mastermix is light sensitive. Wrap the tube in foil to protect from light.", "Reage... |
23,559 | Heat tolerance in plant leaves | null | dx.doi.org/10.17504/protocols.io.29fgh3n | null | Indira V Leon-Garcia, Eloisa Lasso | TITLE: Heat tolerance in plant leaves
AUTHORS: Indira V Leon-Garcia, Eloisa Lasso
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The protocol is based on Krause (2010) with some modifications proposed by the Feeley Lab in 2016. This protocol is for finding heat tolerance or the critical therm... | ["[Plant collection]\nCollect young fully expanded leaves (sample should be enough to cut 9-10 leaf disks).\n- Make sure after the collection and before the heating that your samples are kept well hydrated. Ideally cut them under water in order to maintain the water column and keep it that way until leaf disks are cutt... |
null | null | null | dx.doi.org/10.17504/protocols.io.ncadase | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes the complete protocol for performing N-Glycoproteomics from plasma samples. Albumin removal is performed prior to protein digestion with trypsin. Tryptic peptide mixture is used for enriching N-glycopeptides by lectin-affinity chromatography using a le... | [] |
73,851 | CUTAC for FFPEs | 1 | dx.doi.org/10.17504/protocols.io.14egn292zg5d/v1 | https://www.protocols.click/view/cutac-for-ffpes-ckc3usyn | Steven Henikoff | TITLE: CUTAC for FFPEs
AUTHORS: Steven Henikoff
[DESCRIPTION]
For more than a century, Formalin Fixed Paraffin Embedded (FFPE) sample preparation has been the preferred method for long-term preservation of biological material. However, the use of FFPE samples for epigenomic studies has been difficult because of chroma... | ["[REAGENT SETUP (for up to 16 samples)] Cross-link reversal buffer Mix 800 µL Tris-HCl pH8.0, 195 µL dH2O and 5 µL Triton-X100.\n\nBinding buffer Mix 200 μl 1M HEPES-KOH pH 7.9*, 100 μl 1M KCl, 10 μl 1M CaCl2 and 10 μl 1M MnCl2, and bring the final volume to 10 mL with dH2O. Store the buffer at 4 °C for up to several ... |
null | null | null | dx.doi.org/10.17504/protocols.io.md2c28e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<aside>
<p>Currently, most single cell protocols require the preparation of a single cell suspension from fresh tissue, a major roadblock to clinical deployment, to archived materials and to certain tissues such as adult brain. In the adult brain the harsh enzymatic dissociation... | [] |
20,324 | U Mass - Organ-specific glucose uptake | null | dx.doi.org/10.17504/protocols.io.x4cfqsw | null | Jason Kim | TITLE: U Mass - Organ-specific glucose uptake
AUTHORS: Jason Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block"><span>Glucose uptake in individual organs can be measured using a bolus injection of 2-deoxy-D-[1-</span>... | ["Survival surgery is performed to establish a chronic indwelling catheter at 5~6 days prior to experiment for intravenous infusion. (refer to M1023: Surgery-jugular vein cannulation)", "Mice are fasted overnight (~15 hours) or for 5 hours prior to the start of experiment.", "Place a mouse in a rat-size restrainer wit... |
48,388 | immunoblotting (JMS protocol) | 4 | dx.doi.org/10.17504/protocols.io.bthcnj2w | https://www.protocols.io/view/immunoblotting-jms-protocol-bthcnj2w | Veerle Baekelandt | TITLE: immunoblotting (JMS protocol)
AUTHORS: Veerle Baekelandt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">imonoblotting protocol JMS</div></div>
[STEPS]
?. determination of protein content by a bicinchoninic acid (BCA) assay
?. cell extracts containing 15 µg of total protein were separated on... | ["determination of protein content by a bicinchoninic acid (BCA) assay", "cell extracts containing 15 µg of total protein were separated on 4-15% sodium dodecyl sulfate-polyacrylamide gradient (150V, Bio-Rad) gels", "electroblot gel onto polyvinylidene difluoride membrane", "incubate membranes with paraformaldehyde f... |
null | null | null | dx.doi.org/10.17504/protocols.io.cjtunm | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocols is for PCR using Q5® High-Fidelity DNA Polymerase (M0491)
[GUIDELINES]
<strong>Please note that protocols with Q5® Hot Start High- Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions recommended below should be used for op... | [] |
58,396 | Taxon group: Larval stage insects (TSS2) | 1 | dx.doi.org/10.17504/protocols.io.bp2l61981vqe/v1 | https://www.protocols.io/view/taxon-group-larval-stage-insects-tss2-b494qz8w | Lyndall Pereira da Conceicoa, Olga Sivell, Laura Sivess, Chris Fletcher, Gavin R. Broad, Liam Crowley, Inez Januszczak | TITLE: Taxon group: Larval stage insects (TSS2)
AUTHORS: Lyndall Pereira da Conceicoa, Olga Sivell, Laura Sivess, Chris Fletcher, Gavin R. Broad, Liam Crowley, Inez Januszczak
[DESCRIPTION]
This is part of the collection "DToL Taxon-specific Standard Operating Procedure (SOP) for the Terrestrial and Freshwater Arthrop... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.r6xd9fn | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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?. | ["Weigh out agar 1 g per 100 ml of food to be prepared", "Weigh out 0.022 g sugar, 2.719 g treacle, 7.938 g yeast and 7.321 g semolina per 100 ml of food for the 1:2 P:C diet, or 0.934 g sugar, 14.596 g treacle, 1.285 g yeast and 1.185 g semolina per 100 ml of food for the 1:16 P:C diet", "Measure out water into large ... |
42,844 | Ligation | 1 | dx.doi.org/10.17504/protocols.io.bm34k8qw | https://www.protocols.io/view/ligation-bm34k8qw | Lizhen Zhu | TITLE: Ligation
AUTHORS: Lizhen Zhu
[DESCRIPTION]
Method of plasmid construction connection (T4 DNA ligase)
[GUIDELINES]
teacher ZHANG
[STEPS]
SECTION: system
1. 1.5 µL
4.5 µL
0.5 µL
2 µL
12.5 µL
SECTION: step1
2. Set up a system as required, all operations on ice Mix ligation system with 100µL of competent a... | ["[system] 1.5 µL \n4.5 µL \n0.5 µL \n2 µL \n12.5 µL", "[step1] Set up a system as required, all operations on ice Mix ligation system with 100µL of competent and ice bath for at least 30min (thorough contact of DNA and cell, enabling DNA to get inside cell walls)", "[step2] 42˚C heat shock for 90s (competent cell swel... |
39,851 | Concentration of viruses from wastewater influent using organic flocculation (PEG) | 1 | dx.doi.org/10.17504/protocols.io.bi6jkhcn | https://www.protocols.io/view/concentration-of-viruses-from-wastewater-influent-bi6jkhcn | Katy Graham, Marlene Wolfe, Stephanie Loeb, Alexandria Boehm, Krista Wigginton | TITLE: Concentration of viruses from wastewater influent using organic flocculation (PEG)
AUTHORS: Katy Graham, Marlene Wolfe, Stephanie Loeb, Alexandria Boehm, Krista Wigginton
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This SOP describes pre-analytical procedures to be followed for the isolat... | ["[Concentrate: 3 hours on day 1 and 1 hour on day 2 for 8 samples]\nRemove chosen samples from freezer storage, and thaw at 4°C. This should take about five hours for a 50 mL conical tube, and samples should not be placed at 4°C longer than 24 hrs prior to processing. Record the sample’s volume.\nFollow safe biosafety... |
28,203 | Video Lecturing in Clicker-assisted English Flipped Class | null | dx.doi.org/10.17504/protocols.io.7sjhncn | null | zhonggen yu | TITLE: Video Lecturing in Clicker-assisted English Flipped Class
AUTHORS: zhonggen yu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Value: The study onthe impact of video lecturing on clicker-assistedEnglishflipped class was necessary because it has seldom been explored.Purpose: This study s... | [] |
78,537 | Data analysis code for bioinformatics and statistics | 1 | dx.doi.org/10.17504/protocols.io.dm6gpjm9dgzp/v1 | https://www.protocols.io/view/data-analysis-code-for-bioinformatics-and-statisti-cqxhvxj6 | null | TITLE: Data analysis code for bioinformatics and statistics
AUTHORS:
[DESCRIPTION]
The protocol details the data analysis code for bioinformatics and statistics.
[STEPS] | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.s2hegb6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Overview of the fragment antigen binding</p>
<p>The fragment antigen binding (Fab fragment) is an antibody structure which still binds to antigens although it is monovalent with no Fc portion. Antibody that is digested by the enzyme papain will yields two fab fragments of abo... | [] |
65,268 | Iron Warrior Testo Thrust – Facts & Benefits | 1 | dx.doi.org/10.17504/protocols.io.kqdg3pn27l25/v1 | https://www.protocols.io/view/iron-warrior-testo-thrust-facts-amp-benefits-cbyuspww | irontestoya | TITLE: Iron Warrior Testo Thrust – Facts & Benefits
AUTHORS: irontestoya
[DESCRIPTION]
Iron Warrior Testo ThrustPills are here to assist you with getting monstrous in bed. What's more, they can assist you with having an enormously extraordinary presentation, as well! In the event that you're not feeling so hot i... | [] |
21,779 | Subliminal emotional pictures are capable to modulate early cerebral responses to pain in fibromyalgia | null | dx.doi.org/10.17504/protocols.io.zhtf36n | null | Irene Peláez, David Ferrera, Paloma Barjola, Roberto Fernandes, Francisco Mercado | TITLE: Subliminal emotional pictures are capable to modulate early cerebral responses to pain in fibromyalgia
AUTHORS: Irene Peláez, David Ferrera, Paloma Barjola, Roberto Fernandes, Francisco Mercado
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Participants</spa... | [] |
97,571 | eDNA sample collection method comparison | 0 | null | https://www.protocols.io/view/edna-sample-collection-method-comparison-dbib2kan | Anya Kardailsky, Ryan East | TITLE: eDNA sample collection method comparison
AUTHORS: Anya Kardailsky, Ryan East
[DESCRIPTION]
There is an urgent global need for novel approaches to monitor our freshwater ecosystems. Current biomonitoring tools are time-consuming, costly, and dependent on taxonomic specialists. Therefore, traditional methods are ... | ["[Sample collection and preservation] Wilderlab sampling kits with 0.45µm cellulose nitrate filter columns https://www.wilderlab.co.nz/order", "[Sample collection and preservation] Physico-chemical water tests were done using YSI Pro2030 Dissolved Oxygen and Conductivity Meter and collecting 50ml falcon tubes for nitr... |
52,886 | FindingNemo Extraction 4: Circulomics Kit | 1 | dx.doi.org/10.17504/protocols.io.bxvwpn7e | https://www.protocols.io/view/findingnemo-extraction-4-circulomics-kit-bxvwpn7e | Inswasti Cahyani, John Tyson, Nadine Holmes, Josh Quick, Nicholas Loman, Matthew Loose | TITLE: FindingNemo Extraction 4: Circulomics Kit
AUTHORS: Inswasti Cahyani, John Tyson, Nadine Holmes, Josh Quick, Nicholas Loman, Matthew Loose
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is a sub-protocol designed to extract/isolate ultra-high molecular weight (UHMW) DNA to obtain u... | ["[UHMW DNA Extraction]\nFollow the manufacturer’s instructions as described here.", "[UHMW DNA Extraction]\nStore at 4oC or continue to UL Library Preparation as per Section \"Modified ULK001\". If only SQK-RAD004 is available, follow library preparation as in Section \"Modified RAD004\".\n[for storage]", "Store at 4o... |
61,418 | Immunofluorescence staining | 4 | dx.doi.org/10.17504/protocols.io.36wgq7oq5vk5/v1 | https://www.protocols.io/view/immunofluorescence-staining-b78irrue | michela.deleidi, Pascale Baden, María José Pérez J., Hariam Raji, Federico Bertoli | TITLE: Immunofluorescence staining
AUTHORS: michela.deleidi, Pascale Baden, María José Pérez J., Hariam Raji, Federico Bertoli
[DESCRIPTION]
This protocol describes the immunofluorescence staining of cells.
[STEPS]
SECTION: Fixation of the cells: Strategy 1, i.e., HEK cells
1. Remove medium.
SECTION: Fixation of th... | ["[Fixation of the cells: Strategy 1, i.e., HEK cells] Remove medium.", "[Fixation of the cells: Strategy 1, i.e., HEK cells] Wash with 1× PBS.", "[Fixation of the cells: Strategy 1, i.e., HEK cells] Remove PBS and add 4% PFA to the cells.", "[Fixation of the cells: Strategy 1, i.e., HEK cells] Incubate 10 min at Room ... |
null | null | null | dx.doi.org/10.17504/protocols.io.sd6ea9e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>PNGase F cleaves N-linked (asparagine-linked) oligosaccharides from glycoproteins. The enzyme deaminates asparagine to aspartic acid, leaving the oligosaccharides intact. Denaturation increases the rate of cleavage. Most native proteins can still be completely N-deglycosylate... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.e9ibh4e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is part of the <a href="https://www.protocols.io/view/Collection-of-FOCUS-SubCell-Protocols-For-the-Enri-e9ebh3e" target="_blank">collection</a> of FOCUS™ SubCell protocols for the enrichment of subcellular fractions. Please refer to the appropriate protocol depending on... | [] |
88,794 | Dual-Choice Chamber Assay | 4 | dx.doi.org/10.17504/protocols.io.x54v9p1j4g3e/v1 | https://www.protocols.io/view/dual-choice-chamber-assay-c2x2yfqe | Natalie L. Kendziorski, Tse-Yu Chen, Sara Farless, Sandra A. Allan, Chelsea T Smartt | TITLE: Dual-Choice Chamber Assay
AUTHORS: Natalie L. Kendziorski, Tse-Yu Chen, Sara Farless, Sandra A. Allan, Chelsea T Smartt
[DESCRIPTION]
The protocol describes a laboratory assay to test the repellency of an
essential oil using a modified dual-choice chamber assay system. The assay
assesses the movement of the m... | [] |
45,062 | Quick Protocol for Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030) | 1 | dx.doi.org/10.17504/protocols.io.bp9emr3e | https://www.protocols.io/view/quick-protocol-for-monarch-pcr-dna-cleanup-kit-5-g-bp9emr3e | New England Biolabs | TITLE: Quick Protocol for Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030)
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is the "quick" version of Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030). For the full protocol, please click </span><a hr... | ["Dilute sample with DNA Cleanup Binding Buffer according to the table below. Mix well by pipetting up and down or flicking the tube. Do not vortex. ABC1Sample TypeRatio of Binding Buffer: SampleExample2dsDNA > 2 kb (plasmids, gDNA)2:1200 μl: 100 μl3dsDNA < 2 kb (some amplicons, fragments)5:150... |
70,401 | Growth_of_Pseudomonas_fluorescence_SBW25_in_48-well_Plate_Format | 4 | dx.doi.org/10.17504/protocols.io.j8nlkwewdl5r/v1 | https://www.protocols.io/view/growth-of-pseudomonas-fluorescence-sbw25-in-48-wel-cgy9txz6 | Rosemarie Wilton | TITLE: Growth_of_Pseudomonas_fluorescence_SBW25_in_48-well_Plate_Format
AUTHORS: Rosemarie Wilton
[DESCRIPTION]
This protocol provides a method for culturing Pseudomonas fluorescens SBW25 in 48-well microplates. The protocol is specific for use with a BioTek Epoch 2 plate reader, but other plate readers can be used ... | ["[Culturing Pseudomonas fluorescens SBW25] DAY1: Overnight culture\nInoculate 5 mL LB, in a vented or loosely capped culture tube (e.g. CELLTREAT 50 mL Bio-Reaction tube), from a glycerol stock or with a single colony from a plate.\nGrow overnight at 30 °Cwith shaking at .\n\nDAY2: Four hour culture\nNOTE: Pseudom... |
null | null | null | dx.doi.org/10.17504/protocols.io.gmabu2e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<div>
<div>
<div class="b-start-block editable-content">
<p>This protocol is for the whole genome de novo assembly and annotation for apostichopus japonicus genome, but can be useful in other marine invertebrate genomes. It accompanies the following <em>GigaScience</em> pu... | [] |
52,201 | Co-immunoprecipitation in human cells | 4 | null | https://www.protocols.io/view/co-immunoprecipitation-in-human-cells-bw8hpht6 | Michael Tellier | TITLE: Co-immunoprecipitation in human cells
AUTHORS: Michael Tellier
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol to perform co-immunoprecipitation (co-IP) in human cells. If the co-IP is followed by proteomics, it is better to remove the glycerol from the different buffers.</div></div>... | ["[Day 1]\nSplit human cells in a 150 mm dish to obtain a ~80% confluence on Day 2.Preblock Dynabeads protein A and/or G or Dynabeads M-280 Sheep anti-mouse IgG with 1 ml 0.5% BSA in PBS overnight on a rotating wheel at 16 rpm at 4°C. For Dynabeads protein A and G (30 mg/ml): use 40 μl of beads per condition (at least ... |
22,477 | Buprenorphine to Improve HIV Care Engagement and Outcomes (BRAVO) Randomized Trial: Baseline analysis protocol by C. King (P. Korthuis team) | null | dx.doi.org/10.17504/protocols.io.z7mf9k6 | null | Caroline King | TITLE: Buprenorphine to Improve HIV Care Engagement and Outcomes (BRAVO) Randomized Trial: Baseline analysis protocol by C. King (P. Korthuis team)
AUTHORS: Caroline King
[STEPS]
?. [Overview]
This protocol is for data analysis as part of the Buprenorphine to Improve HIV Care Engagement and Outcomes (BRAVO) Randomized... | ["[Overview]\nThis protocol is for data analysis as part of the Buprenorphine to Improve HIV Care Engagement and Outcomes (BRAVO) Randomized Trial. The BRAVO trial enrolled patients with HIV and moderate-to-severe opiate use disorder to receive either methadone maintenance therapy (MMT) or buprenorphine/naloxone (BUP/N... |
33,928 | Capped RNA Synthesis (E2050) | 1 | dx.doi.org/10.17504/protocols.io.bddgi23w | https://www.protocols.io/view/capped-rna-synthesis-e2050-bddgi23w | New England Biolabs | TITLE: Capped RNA Synthesis (E2050)
AUTHORS: New England Biolabs
[DESCRIPTION]
The kit formulation allows for efficient capped RNA synthesis using cap analog (ARCA).
[BEFORE_START]
We strongly recommend wearing gloves and using nuclease-free tubes and reagents to avoid RNase contamination. Reactions are typically ... | ["Prepare 40 Milimolar (mM).", "Thaw the necessary kit components, mix and pulse-spin in a microfuge to collect solutions to the bottoms of tubes.", "Assemble the reaction at Room temperature in the following order:", "Mix thoroughly and pulse-spin.", "Incubate at 37 °C for 120 min.", "Optional step: To remove template... |
20,108 | Equine parietal bone harvesting | null | dx.doi.org/10.17504/protocols.io.xvkfn4w | null | Lilibeth A Zambrano M, David Kilroy, Arun Kumar, Michael D. Gilchrist, Aisling Ni Anaidh | TITLE: Equine parietal bone harvesting
AUTHORS: Lilibeth A Zambrano M, David Kilroy, Arun Kumar, Michael D. Gilchrist, Aisling Ni Anaidh
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is the procedure carried out to etract beam-like specimens from the parietal bone of horse skull.</div></div>
... | ["[Braincase defleshing]\nIn a room at 22°C:- Using a scalpel, carefully remove soft tissue removed from the bone.- Remove the soft organs attached to the inner surface of the bone (brain, cerebellum, dura mater, etc.).- Permanently irrigate the bone with saline solution.", "[Pre-sampling template]\n- With the pencil, ... |
null | null | null | dx.doi.org/10.17504/protocols.io.j8acrse | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>For RNA extraction, the tissues were ground and the total RNA was isolated using NucleoSpin® RNA columns (Macherey-Nagel; Düren, Germany) according to the manufacturer’s instructions. RNA was quantified using NanoDrop® spectrophotometer ND-1000 (Thermo Scientific Fisher; Walt... | [] |
42,779 | Lloviu cuevavirus sequencing protocol | 1 | null | https://www.protocols.io/view/lloviu-cuevavirus-sequencing-protocol-bmz3k78n | Gábor Tóth, Gabor Kemenesi | TITLE: Lloviu cuevavirus sequencing protocol
AUTHORS: Gábor Tóth, Gabor Kemenesi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">ARTIC-like amplicon sequencing protocol for MinION for Lloviu cuevavirus</div><div class = "text-block">The increasing risk of emerging infectious diseases (EIDs) for huma... | ["[cDNA preparation]\nMix the following components in an 0.2mL 8-strip tube;Component Volume50µM random hexamers 10mM dNTPs mix (10mM each) Template RNA Total\n1 µl\n1 µl\n11 µl\n13 µl\nViral RNA input from a native sample shoul... |
45,261 | Maize hydroponics & Root multiple ion uptake kinetics [sterilization, germination, sowing, growth & uptake] | 1 | dx.doi.org/10.17504/protocols.io.bqfmmtk6 | https://www.protocols.io/view/maize-hydroponics-amp-root-multiple-ion-uptake-kin-bqfmmtk6 | Marcus Griffiths, Sonali Roy, Haichao Guo, Anand Seethepalli, David Huhman, Yaxin Ge, Robert E Sharp, Felix B Fritschi, Larry M York | TITLE: Maize hydroponics & Root multiple ion uptake kinetics [sterilization, germination, sowing, growth & uptake]
AUTHORS: Marcus Griffiths, Sonali Roy, Haichao Guo, Anand Seethepalli, David Huhman, Yaxin Ge, Robert E Sharp, Felix B Fritschi, Larry M York
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-b... | ["[Seed sieving to consistent size]\n(*Optional)For each experiment or series of experiments sieve seeds to mean seed size of parents or population used", "[Seed sterilization (Bleach only method)]\n12 minutes in\n[Bleach (Sodium hypochlorite)]", "[Seed sterilization (Bleach only method)]\nRinse x3 with autoclaved dH2O... |
93,148 | The public attitude towards ChatGPT on Reddit: A study based on unsupervised learning from sentiment analysis and topic modeling | 5 | null | https://www.protocols.io/view/the-public-attitude-towards-chatgpt-on-reddit-a-st-c674zhqw | Zhaoxiang Xu, Mingjian Xie, Yanbo Huang, Qingguo Fang | TITLE: The public attitude towards ChatGPT on Reddit: A study based on unsupervised learning from sentiment analysis and topic modeling
AUTHORS: Zhaoxiang Xu, Mingjian Xie, Yanbo Huang, Qingguo Fang
[DESCRIPTION]
Our code has THREE main parts:
Data Processing: In terms of data cleaning, for the textual data in the d... | ["[Import Packets & Data Preparation] The 4 files below are the processed datasets (with the keywords GPT3.0, GPT3.5, GPT4.0 with the full sample files)\n \n\nThe following 3 files are the original files (with keywords GPT3.0, GPT3.5 & GPT4.0)", "[Data Processing] Data Balancing\n \n\nData Merging (GPT3.0, G... |
null | null | null | dx.doi.org/10.17504/protocols.io.qz3dx8n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Mechanical dissociation of human spleen tissue to single cells for use in 10X chromium scRNA-Seq preparation. </p>
[STEPS]
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63,326 | Trim Clinical Keto | 3 | dx.doi.org/10.17504/protocols.io.rm7vzy142lx1/v1 | https://www.protocols.io/view/trim-clinical-keto-b936r8re | JeffDlton | TITLE: Trim Clinical Keto
AUTHORS: JeffDlton
[DESCRIPTION]
Trim Clinical Keto is a fat burner formula to get you a slim & trim figure Safe For You Buy Now On The Official Website
[STEPS] | [] |
27,550 | Ovary tissue dissociation | 1 | dx.doi.org/10.17504/protocols.io.656hg9e | https://www.protocols.io/view/ovary-tissue-dissociation-656hg9e | Regina Hoo, Carmen Sancho, Roser Vento-Tormo | TITLE: Ovary tissue dissociation
AUTHORS: Regina Hoo, Carmen Sancho, Roser Vento-Tormo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Single cell suspension from ovary tissue and digestion of oocytes</div></div>
[STEPS]
?. [Tissue dissociation and digestion]
Wash tissue with PBS.
?. [Prepare colla... | ["[Tissue dissociation and digestion]\nWash tissue with PBS.", "[Prepare collagenase mix]\nHeat-inactivate FBS at for before use.Collagenase mix recipe: ABCD1ProductStockFinal volume (10 ml)Concentration2RPMI or Hams F12\n+ 10% FBS9 ml RPMI or Hams F12\n+ 1ml FBS8.8 ml3Collagenase IA10 mg/ml1 ml1 mg/ml4Liberase TM5 ... |
69,690 | Setting a sequencing run with the Rapid Barcoding Sequencing gDNA kit (SQK-RBK004) | 4 | dx.doi.org/10.17504/protocols.io.36wgqjr2kvk5/v1 | https://www.protocols.io/view/setting-a-sequencing-run-with-the-rapid-barcoding-cga2tsge | Narjol Gonzalez-Escalona | TITLE: Setting a sequencing run with the Rapid Barcoding Sequencing gDNA kit (SQK-RBK004)
AUTHORS: Narjol Gonzalez-Escalona
[DESCRIPTION]
This protocol is to be used after you mastered setting up a MinION sequencing run (dx.doi.org/10.17504/protocols.io.4r3l2oy53v1y/v3) using the rapid sequencing kit from Nanopore (SQ... | ["[Preparation of the DNA Library:] Thaw all reagents in box 1 and 2 of the RBK-004 kit.", "[Preparation of the DNA Library:] Take a flow cell from 4-8 °C and leave it at room temperature (RT).", "[Preparation of the DNA Library:] Prepare the DNA to a concentration of 400 ng total in 7.5 µL, or a concentration of 54 n... |
85,717 | Sanger Tree of Life HMW DNA Extraction: Pooling | 4 | dx.doi.org/10.17504/protocols.io.3byl4qb4rvo5/v1 | https://www.protocols.io/view/sanger-tree-of-life-hmw-dna-extraction-pooling-cxxvxpn6 | graeme oatley, Filipa Sampaio, Amy Denton, Caroline Howard | TITLE: Sanger Tree of Life HMW DNA Extraction: Pooling
AUTHORS: graeme oatley, Filipa Sampaio, Amy Denton, Caroline Howard
[DESCRIPTION]
This protocol describes the method of combining multiple DNA extractions from the same individual in order to improve the concentration of HMW DNA for PacBio library preparation. Thi... | ["[Procedure] Gather all the DNA eluates that have been produced from multiple DNA extractions from the same individual.", "[Procedure] In a 2 mL microcentrifuge tube, add all DNA to be pooled and pipette mix 5 times with a wide bore pipette. The combined volume of the DNA eluates going into the tube for pooling should... |
103,017 | Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits. | 0 | dx.doi.org/10.17504/protocols.io.3byl495nzgo5/v1 | https://www.protocols.io/view/phosphoglycerate-kinase-is-a-central-leverage-poin-dguh3wt6 | Alexandros C Kokotos, ama, Santiago Unda, Myung Soo Ko, Daehun Park, David Eliezer, Michael G. Kaplitt, Pietro De Camilli, Tim Ryan | TITLE: Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits.
AUTHORS: Alexandros C Kokotos, ama, Santiago Unda, Myung Soo Ko, Daehun Park, David Eliezer, Michael G. Kaplitt, Pietro De Camilli, Tim Ryan
[DESCRIPTION]
The current collection contains all experiment... | [] |
23,807 | Intraperitoneal Glucose Tolerance Testing (IPGTT) | null | dx.doi.org/10.17504/protocols.io.3g7gjzn | null | Donald McClain | TITLE: Intraperitoneal Glucose Tolerance Testing (IPGTT)
AUTHORS: Donald McClain
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">This is the standard protocol for most routine glucose tolerance testing. It is performed... | ["Mice are fasted for six hours by removal to a clean cage without food at the end of their dark (feeding) cycle.", "Mice are weighed.", "After 6h of fasting, a fasting glucose level is obtained from venous blood from a small tail clip.", "1 mg/g body glucose is injected intraperitoneally.", "Blood glucose values are o... |
null | null | null | dx.doi.org/10.17504/protocols.io.ddk24v | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
For <a href="https://www.protocols.io/view/One-step-growth-curves-for-Cellulophaga-phages-ddh235" target="_blank">One-step growth curves for Cellulophaga phages protocol</a>.
[STEPS]
?.
?.
?. | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gqmbvu6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
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48,277 | Treatment regimens used in the management of Helicobacter pylori in Colombia | 1 | dx.doi.org/10.17504/protocols.io.btdvni66 | https://www.protocols.io/view/treatment-regimens-used-in-the-management-of-helic-btdvni66 | Luis Valladales-REstrepo, Yessenia Correa-Sanchez, Brayan Stiven Aristizábal-Carmona, Jorge Machado Alba | TITLE: Treatment regimens used in the management of Helicobacter pylori in Colombia
AUTHORS: Luis Valladales-REstrepo, Yessenia Correa-Sanchez, Brayan Stiven Aristizábal-Carmona, Jorge Machado Alba
[DESCRIPTION]
Background:Helicobacter pylori infection can cause gastritis, gastric ulcers, duodenal ulcers and ga... | [] |
46,020 | 10xV3 Genomics Sample Processing Protocol | 1 | dx.doi.org/10.17504/protocols.io.bq7cmziw | https://www.protocols.io/view/10xv3-genomics-sample-processing-protocol-bq7cmziw | Allen Institute for Brain Science | TITLE: 10xV3 Genomics Sample Processing Protocol
AUTHORS: Allen Institute for Brain Science
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol allows for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells.</div><div class = "text-block"><span style = "fo... | [] |
70,231 | Dual staining for gamma delta and alpha beta T cell detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues | 4 | dx.doi.org/10.17504/protocols.io.q26g7yro1gwz/v1 | https://www.protocols.io/view/dual-staining-for-gamma-delta-and-alpha-beta-t-cel-cgtxtwpn | Jayne E Wiarda | TITLE: Dual staining for gamma delta and alpha beta T cell detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues
AUTHORS: Jayne E Wiarda
[DESCRIPTION]
A combined staining protocol for CD3e immunohistochemistry (IHC) and TRDC RNA in-situ hybridization (RNA-ISH) created for in-situ identification of porcine ... | ["[Baking] Before baking slides:\nPreheat a dry oven to 60℃\nLoad slides for assay into vertical slide rack\n\nBake slides 30 min 60℃\nOptional stopping point: store slides in a dry place & use within 1 week\n\nWhile slides bake:\nPrepare 0.05% PBS-T (can store at RT up to 1 month)\nPrepare 1X Co-Detection Target Retri... |
null | null | null | dx.doi.org/10.17504/protocols.io.q2cdyaw | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
<p><strong>REAGENT PREPARATION</strong></p>
<p> </p>
<p>★<strong> Laird’s buffer (100ml) </strong>(adjust pH to 8,5) [store at room temperature]</p>
<p> </p>
<p>Tris 1,21 g</p>
<p>EDTA-NA<sub>2 </sub>0,19 g</p>
<p>NaCl ... | [] |
88,424 | JHM-MSMP Muscle Flow Cytometry | 1 | dx.doi.org/10.17504/protocols.io.kqdg3xqwqg25/v2 | https://www.protocols.io/view/jhm-msmp-muscle-flow-cytometry-c2kgyctw | ccherry | TITLE: JHM-MSMP Muscle Flow Cytometry
AUTHORS: ccherry
[DESCRIPTION]
Muscle flow cytometry
[STEPS]
SECTION: Protocol
1. Prepare viability dye solution for 100 μL / well to be stained. Dilute stock dyes in PBS.
SECTION: Protocol
2. Spin down plate at 400g for 5 min at 4°C or room temp.
SECTION: Protocol
3. Resuspend c... | ["[Protocol] Prepare viability dye solution for 100 μL / well to be stained. Dilute stock dyes in PBS.", "[Protocol] Spin down plate at 400g for 5 min at 4°C or room temp.", "[Protocol] Resuspend cells in 100μL of appropriate viability dye solution or 100μL of PBS for Unstained samples, all non-viability single-color c... |
55,385 | Emir's methanol fixation IF protocol | 4 | null | https://www.protocols.io/view/emir-39-s-methanol-fixation-if-protocol-b2bzqap6 | Emir Bora Akmeriç | TITLE: Emir's methanol fixation IF protocol
AUTHORS: Emir Bora Akmeriç
[DESCRIPTION]
Methanol fixation protocol for immunofluorescence, should hopefully work for Claudin-5 staining.
[STEPS]
SECTION: Fixation
1. Bring methanol to -20C and prepare compartment in cell culture freezer to quickly transfer slides/dish... | ["[Fixation] Bring methanol to -20C and prepare compartment in cell culture freezer to quickly transfer slides/dishes. Get a box of ice-20 °C", "[Fixation] Incubate sample in methanol for 12 minutes.", "[Fixation] Wash samples with PBS 3 times on ice", "[Fixation] Incubate samples with PBS on ice for 5 minutes, repeat ... |
null | null | null | dx.doi.org/10.17504/protocols.io.m4yc8xw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p> This is a protocol especielly for the isolation of Viral Like Particles (VLP) from the tissues of Molluscs, such as abalone, oyster, clam, scallop and so on. This maybe also applicable for other animal tissues, such as shrimp and fish, based on the principle of the protocol... | [] |
66,638 | Instructor Handbook | 3 | null | https://www.protocols.io/view/instructor-handbook-cdbns2me | Brian Teague | TITLE: Instructor Handbook
AUTHORS: Brian Teague
[DESCRIPTION]
This document describes the theory behind the Yeast ORFans CURE and addresses operational details. Actual instructor protocols (for setting up the student labs) are in the same workspace as this document.
[STEPS] | [] |
48,436 | Expression and purification protocol of the Human (Homo sapiens) mCherry-LC3B Ubiquitin-like modifier | 4 | dx.doi.org/10.17504/protocols.io.btiunkew | https://www.protocols.io/view/expression-and-purification-protocol-of-the-human-btiunkew | Dorotea Fracchiolla | TITLE: Expression and purification protocol of the Human (Homo sapiens) mCherry-LC3B Ubiquitin-like modifier
AUTHORS: Dorotea Fracchiolla
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes expression and purification procedures for obtaining mCherry-tagged human recombinant Ubiq... | ["[Protein Expression]\nTransform plasmid DNA (Addgene 169168 , SMC948) into E.Coli Rosetta pLyss cells and plate on Ampicillin/Chloramphenicol LB agar plate for at .\n37 °C", "[Protein Expression]\nThe following day, inoculate a with 1-2 colonies and grow at shaking.\n[LB + Amp/Cam pre-culture ]\n37 °C", "[Protei... |
71,447 | Health Effects of Vitamin D: Evidence from Mendelian Randomization studies | 1 | dx.doi.org/10.17504/protocols.io.eq2ly7nzmlx9/v1 | https://www.protocols.io/view/health-effects-of-vitamin-d-evidence-from-mendelia-chzxt77n | Aiping Fang, Yue Zhao, Ping, Xuehong, Edward Gio | TITLE: Health Effects of Vitamin D: Evidence from Mendelian Randomization studies
AUTHORS: Aiping Fang, Yue Zhao, Ping, Xuehong, Edward Gio
[DESCRIPTION]
Preclinical data and observational studies suggest that vitamin D has wide-ranging skeletal and extra-skeletal effects. However, it remains controversial whether the... | ["[Background] There is an ongoing debate about the skeletal and extraskeletal effects of vitamin D. A large number of epidemiological studies have been conducted but yield conflicting results1. Although randomized controlled trials (RCTs) are considered the gold standard to identify causality, a large RCT with disease... |
68,565 | Preparation and cryosectioning of fixed mouse brains | 1 | dx.doi.org/10.17504/protocols.io.3byl4bmeovo5/v1 | https://www.protocols.io/view/preparation-and-cryosectioning-of-fixed-mouse-brai-ce7vthn6 | Benjamin Trist, Courtney Wright, Alejandra Rangel | TITLE: Preparation and cryosectioning of fixed mouse brains
AUTHORS: Benjamin Trist, Courtney Wright, Alejandra Rangel
[DESCRIPTION]
This protocol describes how to prepare thin, fixed mouse brain tissue sections from whole fixed mouse brains in preparation for immunohistochemistry or histology. This process includes i... | ["[Cryoprotection and snap freezing] Remove fixed brain from 50 mL sample container and discard 4% paraformaldehyde solution into appropriate waste disposal stream.", "[Cryoprotection and snap freezing] Place brain back into the 50 mL sample container and fill with 30% sucrose in 1x PBS containing 0.01% sodium azide.",... |
82,811 | Generation of membrane tubules by lipid-covered silica beads rolling | 4 | dx.doi.org/10.17504/protocols.io.n92ldpoe7l5b/v1 | https://www.protocols.io/view/generation-of-membrane-tubules-by-lipid-covered-si-cu43wyyn | Javier Espadas, Aurelien Roux | TITLE: Generation of membrane tubules by lipid-covered silica beads rolling
AUTHORS: Javier Espadas, Aurelien Roux
[DESCRIPTION]
This protocol explains the high throughput methodology to generate membrane tubules from lipid-covered silica beads.
[STEPS]
SECTION: Protocol
1. Mix of DOPC, DOPS and Atto 647N DOPE at 59.... | ["[Protocol] Mix of DOPC, DOPS and Atto 647N DOPE at 59.9:40:0.1 mol% respectively in a final volume of 200 µL with chloroform and 0.5 g/L lipid final concentration in a glass vial.", "[Protocol] Dry the lipid mixture in the glass vials for 120 min in a vacuum chamber forming the dried lipid films on the bottom of the ... |
41,280 | NowAware: Rapid Enzyme Activity Detection (REaD) of SARS-CoV-2 | 4 | dx.doi.org/10.17504/protocols.io.bki8kuhw | https://www.protocols.io/view/nowaware-rapid-enzyme-activity-detection-read-of-s-bki8kuhw | prai | TITLE: NowAware: Rapid Enzyme Activity Detection (REaD) of SARS-CoV-2
AUTHORS: prai
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the step-by-step procedure to perform the Rapid Enzyme Activity Detection (REaD) test for SARS-CoV-2 detection from NowAware, Inc. </div></di... | ["[Specimen Collection and Preparation]\nAssure the following testing components for pREaD test are located at the testing lab and are consistent with the following specifications and storage guidelines:Sample Container – This container needs to house the Buffer, Substrate, and Sample Collection tool while also allowin... |
45,162 | 3.2 Isolation of Specific Cell Types from Digested Lung Tissue | 4 | dx.doi.org/10.17504/protocols.io.bqcimsue | https://www.protocols.io/view/3-2-isolation-of-specific-cell-types-from-digested-bqcimsue | Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, Sterghios A. Moschos | TITLE: 3.2 Isolation of Specific Cell Types from Digested Lung Tissue
AUTHORS: Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, Sterghios A. Moschos
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The clinical potential of DNA and RNA-targeting therapeutics for airways disease has be... | ["[3.2 Isolation of Specific Cell Types from Digested Lung Tissue]\nDue to the high intrinsic autofluorescence of tissue resident macrophages which can make flow cytometric analysis more challenging and limit the choice of fluorochromes, a two-stage separation protocol is advised whereby macrophages and other myeloid c... |
84,382 | Changes in Bone Mineral Density and Incidence of Fractures during Two Years of Low Dose Glucocorticoid Treatment for Rheumatoid Arthritis: Protocol for a Systematic Review and Individual Participant Data Meta-Analysis | 1 | dx.doi.org/10.17504/protocols.io.6qpvr3ombvmk/v1 | https://www.protocols.click/view/changes-in-bone-mineral-density-and-incidence-of-f-cwm6xc9e | Andriko Palmowski | TITLE: Changes in Bone Mineral Density and Incidence of Fractures during Two Years of Low Dose Glucocorticoid Treatment for Rheumatoid Arthritis: Protocol for a Systematic Review and Individual Participant Data Meta-Analysis
AUTHORS: Andriko Palmowski
[DESCRIPTION]
Background
Glucocorticoids (GCs) are regularly used d... | ["[PDF Protocol]"] |
100,999 | Cryopreservation of stem cell derived ventral midbrain neural progenitors | 0 | dx.doi.org/10.17504/protocols.io.14egn6696l5d/v1 | https://www.protocols.io/view/cryopreservation-of-stem-cell-derived-ventral-midb-devf3e3n | Tyra Fraser, Lachlan Thompson | TITLE: Cryopreservation of stem cell derived ventral midbrain neural progenitors
AUTHORS: Tyra Fraser, Lachlan Thompson
[DESCRIPTION]
This protocol outlines the cryopreservation procedure for stem cell derived neural progenitors. It can be used for the cryopreservation and long-term storage of ventral midbrain dopamin... | ["[Experimental procedure] Take Mr. Frosty‱ out of -80 °Cfreezer and keep at Room temperature .\nLabel the cryogenic vials with a label machine to include the following information: cell line, clone number, day of differentiation, freezing date and initials. \nWash cells with PBS -/-\nPut 484 µL of Accutase per cm2 of ... |
null | null | null | dx.doi.org/10.17504/protocols.io.sedeba6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div title="Page 1">
<div>
<div>
<p>Endo H cleaves Asparagine-linked hybrid or high mannose oligosaccharides, but not complex oligosaccharides. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated s... | [] |
99,000 | FUNDIS ONT Sequencing IT/Compute Pop!_OS 22.04 Setup | 5 | null | https://www.protocols.io/view/fundis-ont-sequencing-it-compute-pop-os-22-04-setu-dcwy2xfw | Stephen Douglas Russell, Harte Singer | TITLE: FUNDIS ONT Sequencing IT/Compute Pop!_OS 22.04 Setup
AUTHORS: Stephen Douglas Russell, Harte Singer
[DESCRIPTION]
These are the steps to setting up a Linux system running Ubuntu for ONT Nanopore sequencing, basecalling, and consensus-building of fungal amplicon sequences. Adapted from dx.doi.org/10.17504/protoc... | ["[Preparing a new CPU for MinION Sequencing] Ideally you will be running a linux machine with a Ubuntu distro with an NVIDIA graphics card with at least 8GB of ram. (I am running a 4070Ti) You should have at least 32GB of system ram and a new processor. \n\n \n\n\nMinimum IT requirements for MinION from ONT:", "[Prepa... |
84,579 | single-cell TChIC for Zebrafish | 4 | null | https://www.protocols.io/view/single-cell-tchic-for-zebrafish-cwubxesn | Vivek Bhardwaj, Alberto Griffa, Helena Viñas Gaza, Peter Zeller, Alexander van Oudenaarden | TITLE: single-cell TChIC for Zebrafish
AUTHORS: Vivek Bhardwaj, Alberto Griffa, Helena Viñas Gaza, Peter Zeller, Alexander van Oudenaarden
[DESCRIPTION]
Establishing a cell-type-specific chromatin landscape is critical for the maintenance of cell identity during embryonic development. However, our knowledge of how th... | ["[Embryo Dissociation] Obtain Zebrafish embryos. Grow them at 28.5 °C in a 10cm diameter petri dish in ~10 mL of fresh E3 media in an incubator.", "[Embryo Dissociation] Dechorionation\nTransfer the embryos to a glass beaker with a plastic Pasteur pipette. Remove as much E3 as possible without exposing the embryos to ... |
63,376 | k1 keto reviews Does It Work? What They Won’t Tell You! | 1 | dx.doi.org/10.17504/protocols.io.5jyl89kr8v2w/v1 | https://www.protocols.io/view/k1-keto-reviews-does-it-work-what-they-won-t-tell-b95qr85w | MarcChipman | TITLE: k1 keto reviews Does It Work? What They Won’t Tell You!
AUTHORS: MarcChipman
[DESCRIPTION]
K1 Keto Lifeis a premium supplement that causes ketosis. It only has pure BHB salts in it. This product causes ketosis without requiring any dietary or exercise changes.
[STEPS] | [] |
36,863 | Demuxlet Cell Preparation Protocol | 1 | null | https://www.protocols.io/view/demuxlet-cell-preparation-protocol-bf87jrzn | Woong-Yang Park, Jay Shin, Shyam Prabhakar | TITLE: Demuxlet Cell Preparation Protocol
AUTHORS: Woong-Yang Park, Jay Shin, Shyam Prabhakar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details the preparation of PBMC cells.</div></div>
[STEPS]
?. [Preparation of Reagents and Media]
Prepare appropriate amount of thawing media... | ["[Preparation of Reagents and Media]\nPrepare appropriate amount of thawing media including RPMI + 5% HS + 1% Pen/strep + 1% glutamine and keep it for a maximum of 2 weeks at .\n4 °C", "[Preparation of Reagents and Media]\nPrepare appropriate amount of wash media including RPMI + 10% FBS + 1% Pen/strep + 1% glutamine.... |
62,971 | Via keto Gummies Canada : Buy Your Favourite Product {Hurry Up} Offer Is Limited! | 1 | dx.doi.org/10.17504/protocols.io.eq2lyn1wrvx9/v1 | https://www.protocols.io/view/via-keto-gummies-canada-buy-your-favourite-product-b9q3r5yn | roxyhimp | TITLE: Via keto Gummies Canada : Buy Your Favourite Product {Hurry Up} Offer Is Limited!
AUTHORS: roxyhimp
[DESCRIPTION]
Viaketo Gummies Canada : A significant number of people worldwide are struggling with obesity, making it a widespread issue. When a person is overweight, it is usually difficult to exercise every ... | ["[Via keto Gummies Canada]"] |
null | null | null | dx.doi.org/10.17504/protocols.io.eshbeb6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
85,419 | Passaging of organoids (organoids split into cell clusters) | 4 | null | https://www.protocols.io/view/passaging-of-organoids-organoids-split-into-cell-c-cxnjxmcn | Gabriela Vallejo Flores, Annika Fendler | TITLE: Passaging of organoids (organoids split into cell clusters)
AUTHORS: Gabriela Vallejo Flores, Annika Fendler
[DESCRIPTION]
The goal of this experiment is to split the organoids into cell clusters and induce organoids formation and organoids expansion.
[BEFORE_START]
Prepare the organoids medium one day before... | ["[Organoids Splitting] Remove the supernatan from the well and add 1 mL to release the organoids from the matrigel, 400 x g, 5 min, 4 °C, and aspirate the supernatant and Matrigel \nabove the pellet. Note: For the minoli protocol \"organoids grow in cell suspention\", collect the supernatant and do the centrifugat... |
43,706 | Improve | 1 | null | https://www.protocols.io/view/improve-bnw2mfge | Hung Yu Chen, Huan Jui Chang | TITLE: Improve
AUTHORS: Hung Yu Chen, Huan Jui Chang
[STEPS]
?. [In vitro protIn vitro protein synthesis]
Add the reagents(on ice) for synthesis of amilCP into 15 wells of the 384-well plate. Pipette well before adding reagents into the wells.banana amilCP ABCD1OrderLocationReagentAmount21Upper-leftDNase/ RNase free... | ["[In vitro protIn vitro protein synthesis]\nAdd the reagents(on ice) for synthesis of amilCP into 15 wells of the 384-well plate. Pipette well before adding reagents into the wells.banana amilCP ABCD1OrderLocationReagentAmount21Upper-leftDNase/ RNase free watertill 5 μL32Upper-leftSolution A2 μL43Lower-rightSolution ... |
35,718 | Non-invasive sublingual glycocalyx measurements using GlycoCheck (RRID:SCR_018254) | 1 | dx.doi.org/10.17504/protocols.io.be5ejg3e | https://www.protocols.io/view/non-invasive-sublingual-glycocalyx-measurements-us-be5ejg3e | Tracey Weissgerber, Oscar Garcia Valencia | TITLE: Non-invasive sublingual glycocalyx measurements using GlycoCheck (RRID:SCR_018254)
AUTHORS: Tracey Weissgerber, Oscar Garcia Valencia
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes procedures for non-invasive measurements of sublingual vascular glycocalyx degradation ... | ["[Set-up (to be completed before the patient is tested)]\nTake out the computer and camera.", "[Set-up (to be completed before the patient is tested)]\nPlug camera into a USB port on the right-hand side of the computer", "[Set-up (to be completed before the patient is tested)]\nTurn on the computer", "[Set-up (to be c... |
70,686 | Quantification of various SARS-CoV-2 variant mutations (characteristic of Alpha, Beta, Gamma, Delta, Omicron and Omicron sublineages) in settled solids using digital RT-PCR | 1 | dx.doi.org/10.17504/protocols.io.14egnzrrzg5d/v9 | https://www.protocols.io/view/quantification-of-various-sars-cov-2-variant-mutat-cg96tz9e | Bridgette Hughes, Bradley J. White, Marlene K. Wolfe, Alexandria B Boehm | TITLE: Quantification of various SARS-CoV-2 variant mutations (characteristic of Alpha, Beta, Gamma, Delta, Omicron and Omicron sublineages) in settled solids using digital RT-PCR
AUTHORS: Bridgette Hughes, Bradley J. White, Marlene K. Wolfe, Alexandria B Boehm
[DESCRIPTION]
This process instruction describes the step... | ["[Preparation] Retrieve all kit components from the One-Step RT-ddPCR advanced kit for probes from the -20 °C freezer and thaw the components on ice.", "[Preparation] Retrieve ddPCR positive control aliquots (50 copies per uL gRNA) from the -80 °C freezer and thaw on ice", "[Preparation] For re-running frozen plates o... |
46,453 | Fluorescent immunochemistry | 4 | dx.doi.org/10.17504/protocols.io.brkvm4w6 | https://www.protocols.io/view/fluorescent-immunochemistry-brkvm4w6 | Corentin Cras-Méneur | TITLE: Fluorescent immunochemistry
AUTHORS: Corentin Cras-Méneur
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">General fluorescent immunostaining protocol for paraffin sections</div></div>
[STEPS]
?. [Deparaffinizing]
- - - Ethanol 100° - Ethanol 95° - Ethanol 75°
?. [Antigen Retrieval]
Microw... | ["[Deparaffinizing]\n- - - Ethanol 100° - Ethanol 95° - Ethanol 75°", "[Antigen Retrieval]\nMicrowave for 3 x with microwaving solution (kept @ )Let it cool until\n4 °C\non ice\n0 Room temperature", "[Permeabilization]\nPermeabilization with TBS 1x; Triton 100 (20 min @ )\n0 Room temperature", "[Blocking]\nBlockin... |
null | null | null | dx.doi.org/10.17504/protocols.io.c7azid | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
83,293 | Quick and dirty sequencing microbial genome extraction v1 | 4 | dx.doi.org/10.17504/protocols.io.ewov1qjx7gr2/v1 | https://www.protocols.click/view/quick-and-dirty-sequencing-microbial-genome-extrac-cvj5w4q6 | Sung won Lim, Sebastian Cocioba | TITLE: Quick and dirty sequencing microbial genome extraction v1
AUTHORS: Sung won Lim, Sebastian Cocioba
[DESCRIPTION]
Quick and dirty microbial genome extraction protocol using Edwards buffer.
I've been using this method for in-lab Archaeal evolution study for the last four years or so and it's been working fantas... | ["Spin down 1 ml of sample for 1 minute at max speed and decant", "Resuspend vigorously with 100ul Edward's buffer", "Transfer carefully to PCR tube - mixture will be viscous", "Add 2ul of RNase A and mix vigorously, vortex for 10 seconds", "Incubate at 37C for 15 minutes", "Add 2ul of Proteinase K and mix vigorously, ... |
89,822 | SureSelect XT HS2 DNA to prepare libraries for single-cell Whole Genome Sequencing (scWGS) after single-cell Whole Genome Amplification (scWGA) | 2 | dx.doi.org/10.17504/protocols.io.x54v9p3qzg3e/v1 | https://www.protocols.io/view/sureselect-xt-hs2-dna-to-prepare-libraries-for-sin-c3x6ypre | Ester Kalef-Ezra, Ben Harvey, Katherine Roper, Christos Proukakis | TITLE: SureSelect XT HS2 DNA to prepare libraries for single-cell Whole Genome Sequencing (scWGS) after single-cell Whole Genome Amplification (scWGA)
AUTHORS: Ester Kalef-Ezra, Ben Harvey, Katherine Roper, Christos Proukakis
[DESCRIPTION]
We adapted the SureSelect XT HS2 DNA protocol to prepare libraries using as inp... | [] |
26,872 | Streptavidin dot blot with aptamers | null | dx.doi.org/10.17504/protocols.io.6gyhbxw | null | Manuela de las Casas | TITLE: Streptavidin dot blot with aptamers
AUTHORS: Manuela de las Casas
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The aim of this protocol is to check the efficiency of the detection system that will be present in the strips. The result expected is a good binding between the streptavidin and ... | ["[Preparing the nitrocellulose strip]\nSet the hot plate to 100ºC or at least warm enough to melt the wax. Cut a small amount from one of the wax pencils and place it on a Petri dish. Set the Petri dish on the hot plate and wait for the wax to melt. Cut a strip from the nitrocellulose sheet with the desired size.Once... |
90,862 | QuPath Immunofluorescence Cell Detection and Co-localization Protocol | 5 | dx.doi.org/10.17504/protocols.io.e6nvwdmnwlmk/v1 | https://www.protocols.io/view/qupath-immunofluorescence-cell-detection-and-co-lo-c4ynyxve | Patricia Lumanto, Diana Nakib, Xinle Wang, Sonya Macparland | TITLE: QuPath Immunofluorescence Cell Detection and Co-localization Protocol
AUTHORS: Patricia Lumanto, Diana Nakib, Xinle Wang, Sonya Macparland
[DESCRIPTION]
QuPath is an open-source imaging software that provides a user-friendly platform for digital pathology and whole slide image analysis. This protocol aims to pr... | ["[Image Brightness and Contrast Adjustment] Click on File: Project to start a project.\nNote: a project must be created in order for annotations and measurements to be exported.\nTip: prepare an empty folder with an appropriate folder name prior to starting the project.", "[Image Brightness and Contrast Adjustment] Im... |
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