id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
|---|---|---|---|---|---|---|---|
66,641 | Dual Luciferase Assay | 1 | null | https://www.protocols.io/view/dual-luciferase-assay-cdbrs2m6 | vahedilaboratory | TITLE: Dual Luciferase Assay
AUTHORS: vahedilaboratory
[DESCRIPTION]
Dual luciferase assay WIP
[STEPS]
3. Cell Lysing and Luciferase Assay
Remove media from each well and wash with PBS; remove the PBS
Add 50uL trypsin to each well and coat every cell
Leave in incubator for 3 minutes
Pipette all cells off of plate... | ["Cell Lysing and Luciferase Assay\nRemove media from each well and wash with PBS; remove the PBS\nAdd 50uL trypsin to each well and coat every cell\nLeave in incubator for 3 minutes\nPipette all cells off of plate and add cells to 1.5mL tubes \nSpin down cells at 1,500RPM 4C for 5 minutes to pellet cells \nRemove supe... |
null | null | null | dx.doi.org/10.17504/protocols.io.cn2vgd | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
34,192 | LyGo cloning | 1 | dx.doi.org/10.17504/protocols.io.bdmqi45w | https://www.protocols.io/view/lygo-cloning-bdmqi45w | Kristoffer Bach Falkenberg, Cristina Hernandez Rollan, Maja Rennig, Andreas Birk Bertelsen, Morten Norholm | TITLE: LyGo cloning
AUTHORS: Kristoffer Bach Falkenberg, Cristina Hernandez Rollan, Maja Rennig, Andreas Birk Bertelsen, Morten Norholm
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Lytic polysaccharide monooxygenses (LPMOs) are enzymes that play a critical role in breaking the chemical bonds of t... | ["[Mix reactions]\nPrepare a reaction mix in the following proportions: AB1ComponentVolume (μL)2LyGo vector13LyGo fragment34FastDigest SapI0.5510x T4 ligase1610x FastDigest buffer1710x T4 buffer18MQ2.59Total10\nAB1ComponentVolume (μL)2LyGo vector13LyGo fragment34FastDigest SapI0.5510x T4 ligase1610x FastDigest buffer1... |
100,280 | Integrated laser ablation and droplet collection system for efficient single cell isolation | 0 | dx.doi.org/10.17504/protocols.io.kxygxy3ewl8j/v1 | https://www.protocols.io/view/integrated-laser-ablation-and-droplet-collection-s-dd6y29fw | Liang Chen, Andrey V. Liyu, Yumi Kwon, Ying Zhu, Ljiljana.PasaTolic | TITLE: Integrated laser ablation and droplet collection system for efficient single cell isolation
AUTHORS: Liang Chen, Andrey V. Liyu, Yumi Kwon, Ying Zhu, Ljiljana.PasaTolic
[DESCRIPTION]
State-of-the-art omics and imaging technologies for transcriptome, epigenome, and proteome measurements at the single-cell level ... | ["[Laser capture microdissection (LCM)] Install in-house built robotic arm, which is used in the generation and delivery of a captured droplet.", "[Laser capture microdissection (LCM)] Wash the Syringe and glass capillary with MilliQ water", "[Laser capture microdissection (LCM)] Turn on the PALM MicroBeam system", "[L... |
109,018 | Nested PCR amplification of Salmonella Typhi from extracted wastewater concentrate | 0 | dx.doi.org/10.17504/protocols.io.36wgqnw3ogk5/v1 | https://www.protocols.io/view/nested-pcr-amplification-of-salmonella-typhi-from-dnp25dqe | Shannon Fitz, Alex Shaw, michael Owusu, Dilip Abraham, Anton Spadar | TITLE: Nested PCR amplification of Salmonella Typhi from extracted wastewater concentrate
AUTHORS: Shannon Fitz, Alex Shaw, michael Owusu, Dilip Abraham, Anton Spadar
[DESCRIPTION]
This standard operating procedure describes nested PCR protocols for the generation of amplicons from V. cholerae and S. typhi.
PCR pr... | ["[Primer preparation step] Workstation Preparation\n\nClean the PCR areas before setting up the reactions; clean room for the master mix and an area for the addition of the template. \nClean the working area and pipettors with an approved DNAse inhibitor solution.\nClean the Class II biosafety cabinet and pipettes wit... |
null | null | null | dx.doi.org/10.17504/protocols.io.scpeavn | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol outlines our preparation of single-cell suspension from surgically acquired fresh human adult brain tissue. This method is adapted from Habib et al<sup>1</sup>.</p>
<p> </p>
<p>1 Habib, N.<em> et al.</em> Massively parallel single-nucleus RNA-seq with D... | [] |
67,387 | Bewertungen von Diaetoxil-Kapseln - Effektive Inhaltsstoffe wirken beim Abnehmen! | 1 | dx.doi.org/10.17504/protocols.io.n2bvj645wlk5/v1 | https://www.protocols.io/view/bewertungen-von-diaetoxil-kapseln-effektive-inhalt-cd23s8gn | natedif | TITLE: Bewertungen von Diaetoxil-Kapseln - Effektive Inhaltsstoffe wirken beim Abnehmen!
AUTHORS: natedif
[DESCRIPTION]
➢ Produktname – Diaetoxil
➢ Nebenwirkungen – NA
➢ Zusammensetzung — Natürliche organische Verbindung
➢ Vorteile – Hilft, Gewicht zu reduzieren und die Energie zu steigern!
➢ Verfügbarkeit — Auf Lag... | [] |
20,015 | Preparation of the adenylated oligonucleotide for mapping 3′OH RNA termini | null | dx.doi.org/10.17504/protocols.io.xspfndn | null | Matus Valach | TITLE: Preparation of the adenylated oligonucleotide for mapping 3′OH RNA termini
AUTHORS: Matus Valach
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Adapted from the protocol for oligonucleotide 5′ adenylation (E2610) of </span><span style = "font-style:italic;">New England Biolabs</span><s... | ["Mix the following components (20 μL): ABC1ComponentAmount [μL]Final concentration25′ phosphorylated DNA oligo [50 μM]410 μM310× 5′ DNA Adenylation buffer21×4ATP [1 mM]20.1 mM5Mth RNA ligase [50 pmol/µL]410 µM6RNase-free water (ddH2O)8\nABC1ComponentAmount [μL]Final concentration25′ phosphorylated DNA oligo [50 μM]41... |
27,501 | Effective Lonza 4D Nucleofection with Inexpensive Homemade Buffers | null | dx.doi.org/10.17504/protocols.io.64mhgu6 | https://www.protocols.io/view/effective-lonza-4d-nucleofection-with-inexpensive-64mhgu6 | Eric Danner | TITLE: Effective Lonza 4D Nucleofection with Inexpensive Homemade Buffers
AUTHORS: Eric Danner
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol uses the Lonza 4D nucleofection system. Here I present protocols that reduce the price dramatically of using Lonza 4D equipment. </div><div cl... | ["[Prepare Reagents]\nMake Nucleofection Buffer (AmaxaV): Stocks can be stored RT for a long time (we use ours longer than 6 months). Making working solutoin from stock solution, vacuum filter through 0.45um filter. This should be stored at 4C. Discard after 3 months or when you see white precipitate. ABCDEFG1Stoc... |
21,662 | Analysis of the Derivatives of Sialic Acid in Pig Tissues | null | dx.doi.org/10.17504/protocols.io.zd6f29e | null | Chon-Ho Yen | TITLE: Analysis of the Derivatives of Sialic Acid in Pig Tissues
AUTHORS: Chon-Ho Yen
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. N-glycolylneuraminic acid (Ne... | [] |
51,966 | A Difference-in-Differences Analysis of New Persistent Opioid Use After Surgery | 1 | dx.doi.org/10.17504/protocols.io.bwy6pfze | https://www.protocols.io/view/a-difference-in-differences-analysis-of-new-persis-bwy6pfze | Ryan Howard, Craig Brown, Vidhya Gunaseelan, Michael Englesbe, Jennifer Waljee, Mark Bicket, Andrew Ryan, Chad Brummett | TITLE: A Difference-in-Differences Analysis of New Persistent Opioid Use After Surgery
AUTHORS: Ryan Howard, Craig Brown, Vidhya Gunaseelan, Michael Englesbe, Jennifer Waljee, Mark Bicket, Andrew Ryan, Chad Brummett
[DESCRIPTION]
The Opioid Prescribing Engagement Network (OPEN) at the University of Michigan was establ... | ["[Brief Rationale and Hypothesis] Brief Summary: The Opioid Prescribing Engagement Network (OPEN) at the University of Michigan was established in 2016 with the goal of reducing excessive opioid prescribing after surgery using evidence-based prescribing guidelines. Beginning in July 2016, OPEN began a statewide qualit... |
80,893 | Methodology for Inputs-Oriented VRS DEA in dairy farms | 1 | dx.doi.org/10.17504/protocols.io.ewov1o6z7lr2/v1 | https://www.protocols.io/view/methodology-for-inputs-oriented-vrs-dea-in-dairy-f-cs85why6 | C A Zuniga-Gonzalez, Jaramillo | TITLE: Methodology for Inputs-Oriented VRS DEA in dairy farms
AUTHORS: C A Zuniga-Gonzalez, Jaramillo
[DESCRIPTION]
The methodology protocol for Inputs-Oriented VRS DEA in dairy farms describes the steps and procedures for data collection with the verbal consent of the producers according to the declaration included i... | ["[Protocol for the collection and processing of data with the verbal consent of the producers] The data collection procedure was as follows: a) identification of the regions of the republic with the highest volume of milk production. A questionnaire was designed to collect information from the selected producers. Dur... |
64,710 | Illumina double-stranded DNA dual indexing for ancient DNA | 1 | dx.doi.org/10.17504/protocols.io.4r3l287x3l1y/v3 | https://www.protocols.io/view/illumina-double-stranded-dna-dual-indexing-for-anc-cbfesjje | Raphaela Stahl, Christina Warinner, Irina Velsko, Eleftheria Orfanou, Franziska Aron, Guido Brandt | TITLE: Illumina double-stranded DNA dual indexing for ancient DNA
AUTHORS: Raphaela Stahl, Christina Warinner, Irina Velsko, Eleftheria Orfanou, Franziska Aron, Guido Brandt
[DESCRIPTION]
This protocol converts partially completed double-stranded DNA libraries e.g. from:
Non-UDG treated double-stranded ancient DNA li... | ["[Indexing preparation (aDNA library preparation room)] Calculate the total number of DNA molecules (total copy number) DNA concentration in each library based on qPCR performed at the end of library preparation (see Before Start). Do not use more than 1.5x10^10 copies per indexing reaction. Adjust the amount of DNA u... |
41,758 | C-19 protocol | 4 | null | https://www.protocols.io/view/c-19-protocol-bkz6kx9e | yalhiyari | TITLE: C-19 protocol
AUTHORS: yalhiyari
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A rapid covid screening taking 30sec- 1 min once placed in DOCI machine. Also does not require any biological sample manipulation.</div></div>
[STEPS]
?. Centrifuge: 3500 33, 5 min, 22 10
?. Need pipet tips an... | ["Centrifuge: 3500 33, 5 min, 22 10", "Need pipet tips and 20-200ul pipet, 96well glass bottom plate Fishersci 07-000-633\n[Aliquot 50ul of supernatant 1 well per sample into 96 well glass bottom plate ]", "Stick 96 well plate into machine for viral detection. 30 sec-1 min per run"] |
44,764 | 3.5 Applications of G-Trap Assay | 3 | dx.doi.org/10.17504/protocols.io.bpx4mpqw | https://www.protocols.io/view/3-5-applications-of-g-trap-assay-bpx4mpqw | Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda | TITLE: 3.5 Applications of G-Trap Assay
AUTHORS: Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Small, monomeric guanine triphosphate hydrolases (GTPases) are ubiquitous cellular integrators of signaling. A signal activates th... | [] |
83,933 | SHARE-seq protocol v2.2 | 4 | null | https://www.protocols.io/view/share-seq-protocol-v2-2-cv75w9q6 | Amelia Hall | TITLE: SHARE-seq protocol v2.2
AUTHORS: Amelia Hall
[DESCRIPTION]
TBD
[STEPS]
SECTION: 1.1 Ordering Oligo Plates and oligos for plate production
2. One of the more involved aspects of SHARE-seq (and SPLiT-seq) is properly making the oligo hybridization 96 well plates - this section covers that in detail before moving... | ["[1.1 Ordering Oligo Plates and oligos for plate production] One of the more involved aspects of SHARE-seq (and SPLiT-seq) is properly making the oligo hybridization 96 well plates - this section covers that in detail before moving into any of the day to day aspects of this protocol (those start at step 24, in section... |
33,852 | Ferrous Ion-chelating Activity | null | dx.doi.org/10.17504/protocols.io.bda4i2gw | null | Jing Xu | TITLE: Ferrous Ion-chelating Activity
AUTHORS: Jing Xu
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The effects of ferrous ion-chelating activity were conducted according to Guo et al.An aliquot of 1 ml extract was added to a solution of 100 µL of FeCl</span><span style = "vertical-align:su... | [] |
30,471 | 6-Hydroxydopamine (6-OHDA) Treatment and Neurite Tracing in miDA Neurons | 1 | null | https://www.protocols.io/view/6-hydroxydopamine-6-ohda-treatment-and-neurite-tra-9zfh73n | Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying | TITLE: 6-Hydroxydopamine (6-OHDA) Treatment and Neurite Tracing in miDA Neurons
AUTHORS: Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S. Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>... | ["[Cell Seeding]\nPlate miDA neurons (3 × 103 per well) in a 384-well plate coated with poly-D-lysine and laminin.", "[Calcein AM Assay]\nPrepare fresh 6-OHDA in vehicle solution (0.15% ascorbic acid in H2O).\nControl cells should be treated with vehicle solution alone.", "[Calcein AM Assay]\nIncubate cells with fresh ... |
26,995 | Generation and Purification of pTXB1.Tn5 | 1 | dx.doi.org/10.17504/protocols.io.6kthcwn | https://www.protocols.io/view/generation-and-purification-of-ptxb1-tn5-6kthcwn | Ryan Mulqueen, Andy Fields, Andrew Adey | TITLE: Generation and Purification of pTXB1.Tn5
AUTHORS: Ryan Mulqueen, Andy Fields, Andrew Adey
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Generation of Tn5 transposome, protein purification and loading for the sci- family of protocols.</div><div class = "text-block">Protein purificatio... | ["[Prior to day 1]\nGenerate LB agarose plates with following addgene suggested protocol. https://www.addgene.org/mol-bio-reference/#antibiotics\n[Carbenicillin]", "[Day 1: Grow fresh colonies]\nFreshly streak out a LB agarose plates with from the glycerol stock. Allow to grow overnight at in an incubator.\n[Car... |
84,606 | Roteiro para vídeo-devolutivas de relatórios actigráficos | 1 | dx.doi.org/10.17504/protocols.io.261gedozdv47/v1 | https://www.protocols.click/view/roteiro-para-v-deo-devolutivas-de-relat-rios-actig-cwu6xeze | Daniel Vartanian, Alicia Rafaelly Vilefort Sales, Maria Augusta Medeiros Andrade | TITLE: Roteiro para vídeo-devolutivas de relatórios actigráficos
AUTHORS: Daniel Vartanian, Alicia Rafaelly Vilefort Sales, Maria Augusta Medeiros Andrade
[DESCRIPTION]
Este protocolo foi desenhado para o processo de coleta de dados do projeto de pesquisa Sono e Gravidez. Consulte a aba Guidelines para visualizar o fl... | ["[Preparação] Abra o relatório actigráfico em uma janela única em seu navegador.", "[Preparação] Oculte o menu do Google Docs ao clicar na seta localizada no canto superior direito da página.", "[Preparação] Caso alguém, além de você, esteja visualizando o documento, peça para que eles saiam.", "[Preparação] Abra o Lo... |
49,737 | Discovery proteomic (DDA) LC-MS/MS data acquisition and analysis | 1 | dx.doi.org/10.17504/protocols.io.buthnwj6 | https://www.protocols.io/view/discovery-proteomic-dda-lc-ms-ms-data-acquisition-buthnwj6 | Yan Chen, Jennifer Gin, Christopher Petzold | TITLE: Discovery proteomic (DDA) LC-MS/MS data acquisition and analysis
AUTHORS: Yan Chen, Jennifer Gin, Christopher Petzold
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol details steps in discovery proteomic data-dependent acquisition with a standard-flow UHPLC-Obitrap system a... | ["[Proteomics: HPLC and Mass Spectromtery]\nThaw peptide samples , and transfer of each sample to LC autosampler vials (Agilent, Cat.#5182-0567,#5182-0564) or 96-well plate (Bio-Rad, Cat.#HSP9655).\non ice\n30 µl", "[Proteomics: HPLC and Mass Spectromtery]\nLiquid chromatography-tandem mass spectrometry (LC-MS/MS) an... |
74,988 | P0 PLANIFICACIÓN ANUAL | 1 | dx.doi.org/10.17504/protocols.io.5qpvorpb7v4o/v1 | https://www.protocols.io/view/p0-planificaci-n-anual-cmgku3uw | cgarcia | TITLE: P0 PLANIFICACIÓN ANUAL
AUTHORS: cgarcia
[DESCRIPTION]
Cada curso académico, la EIDUCAM funcionará con un calendario establecido, que se desarrollará en el mes de Mayo del curso anterior.
Fechas a fijar para cada curso académico
[STEPS]
SECTION: P0 PLANIFICACIÓN ANUAL
1. Fechas a fijar para cada curso académic... | ["[P0 PLANIFICACIÓN ANUAL] Fechas a fijar para cada curso académico:\n\nDifusión de la información relativa a los programas de doctorado de la EIDUCAM: Mes de Junio.", "[P0 PLANIFICACIÓN ANUAL] Planificación Programas de Doctorado (Septiembre)\n\na. Oferta de plazas por línea de investigación \nb. Oferta de plazas espe... |
86,891 | Can light be used to treat obesity and diabetes? | 4 | dx.doi.org/10.17504/protocols.io.j8nlkooj1v5r/v3 | https://www.protocols.io/view/can-light-be-used-to-treat-obesity-and-diabetes-cy4jxyun | Rédoane Daoudi | TITLE: Can light be used to treat obesity and diabetes?
AUTHORS: Rédoane Daoudi
[DESCRIPTION]
This document is highly theoretical lab method paper. It may be used to perform thermogenesis from several cells in a living organism instead of only brown adipocytes. We perform thermogenesis from white adipocytes. The purpo... | ["[Introduction] This document is highly theoretical lab method paper. It may be used to perform thermogenesis from several cells in a living organism instead of only brown adipocytes. We perform thermogenesis from white adipocytes. The purpose is to uptake the blood glucose and lipids to produce heat and subsequent we... |
40,492 | Binding properties of Human IgG to immunoglobulin-binding proteins tested by double immunodiffusion (Ouchterlony) technique. | 4 | dx.doi.org/10.17504/protocols.io.bjskkncw | https://www.protocols.io/view/binding-properties-of-human-igg-to-immunoglobulin-bjskkncw | Angel Justiz-Vaillant | TITLE: Binding properties of Human IgG to immunoglobulin-binding proteins tested by double immunodiffusion (Ouchterlony) technique.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">1. IBP-HRP conjugates can be prepared in the laboratory by the periodate method.</div>... | ["The binding of Human IgG to peroxidase-conjugated Immunoglobulin-binding protein (IBP-HRP) including SpA-HRP, SpG-HRP, SpL-HRP, SpLA-HRP, SpAG-HRP, SpLG-HRP, SpLAG-HRP and turtle serum (Sigma-Aldrich) as a negative control are investigated by double immunodiffusion.", "Briefly, 1% agarose gels are prepared and wells... |
null | null | null | dx.doi.org/10.17504/protocols.io.h8vb9w6 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
48,702 | nCoV-2019 sequencing protocol v3 (LoCost) - InvitaeSF v2.0 | 1 | null | https://www.protocols.io/view/ncov-2019-sequencing-protocol-v3-locost-invitaesf-bts6nnhe | Amanda Kahn-Kirby, Lisa Cunden, Bianca Miani [Invitae | TITLE: nCoV-2019 sequencing protocol v3 (LoCost) - InvitaeSF v2.0
AUTHORS: Amanda Kahn-Kirby, Lisa Cunden, Bianca Miani [Invitae
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Amplicon sequencing protocol for SARS-CoV-2 v3 (LoCost), modified for automation and clean yield. Thank you Josh Quick and... | ["[cDNA preparation]\nStamp input plate to a 3-col or 6-col pre-aliquoted RT master mix plate using the Bravo protocol. AB1ComponentVolume2LunaScript RT SuperMix (5X) 2 µL3Normalized Template RNA 8 µL4Total10 µL\nAB1ComponentVolume2LunaScript RT SuperMix (5X) 2 µL3Normalized Template RNA 8 µL4Total10 µL\nViral R... |
70,678 | Isolating bacteria from algal monocultures | 4 | dx.doi.org/10.17504/protocols.io.261ge3okyl47/v1 | https://www.protocols.io/view/isolating-bacteria-from-algal-monocultures-cg9wtz7e | Emily Aguirre | TITLE: Isolating bacteria from algal monocultures
AUTHORS: Emily Aguirre
[DESCRIPTION]
To isolate bacteria from non-axenic, algal (we did this with Symbiodinium but may work with other algae) monocultures. Our aim is to isolate bacterial members of the phycosphere to use in re-inoculation and genome wide association s... | ["[Initial isolation from Symbiodinium] Transfer 1 mL of a non-axenic Symbiodinium culture to a 2mL microcentrifuge tube. Centrifuge the algae and bacteria at 10,000 rpm for 2 minutes.", "[Initial isolation from Symbiodinium]", "[Initial isolation from Symbiodinium]", "[Initial isolation from Symbiodinium]", "[Initial ... |
45,064 | Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020) | 1 | dx.doi.org/10.17504/protocols.io.bp9gmr3w | https://www.protocols.io/view/quick-protocol-for-monarch-dna-gel-extraction-kit-bp9gmr3w | New England Biolabs | TITLE: Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020)
AUTHORS: New England Biolabs
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This is the quick version of the Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020). For the full protocol, please click </span><a href="https:... | ["Excise the DNA fragment from the agarose gel, taking care to trim excess agarose. Transfer to a 1.5 ml microfuge tube and weigh the gel slice. Minimize exposure to UV light.\nTrim/remove excess agarose and minimize exposure to UV light.", "Add 4 volumes of Gel Dissolving Buffer to the gel slice (e.g., 400 μl buffer p... |
108,552 | Designing sgRNA Oligos and Inserting Guides into the GEARBOCS Vector | 0 | dx.doi.org/10.17504/protocols.io.5jyl8271rl2w/v1 | https://www.protocols.io/view/designing-sgrna-oligos-and-inserting-guides-into-t-dm9g493w | Luke Bradley | TITLE: Designing sgRNA Oligos and Inserting Guides into the GEARBOCS Vector
AUTHORS: Luke Bradley
[DESCRIPTION]
This protocol describes designing sgRNAs for genes of interest using the GEARBOCS vector backbone. This method allows researchers to utilize the GEARBOCS system for astrocyte-specific genetic manipulation i... | ["[Designing sgRNA oligos] Use UCSC to get gene's exons and introns. Make sure the area you are targeting is in all isoforms.", "[Designing sgRNA oligos] Before designing your own guides, check the literature for guides that you can use.", "[Designing sgRNA oligos] Second, try CRISPick with the exon you want to target.... |
89,164 | Wnt-3a and R-spo1 conditioned media reporter assay | 4 | dx.doi.org/10.17504/protocols.io.5qpvorz99v4o/v2 | https://www.protocols.io/view/wnt-3a-and-r-spo1-conditioned-media-reporter-assay-c3bkyikw | Natalia Martagón, Gurdrun Kliem | TITLE: Wnt-3a and R-spo1 conditioned media reporter assay
AUTHORS: Natalia Martagón, Gurdrun Kliem
[DESCRIPTION]
Protocol designed to measure the activity of Wnt-3a or R-spondin-1 (Rspo1) conditioned media.
A reporter HEK cell line expressing luciferase under Wnt-3a stimulation is cultured with conditioned media follo... | ["[Day 2: cell stimulation] Aspirate medium and discard.\n\nAdd a total of 500 µL of HEK medium with the following amount of conditioned medium (CM) to test:\nFor R-spondin CM: 12.5 % volume Wnt-3a CM + 2.5 % volume Rspo1 CM (3)\nFor Wnt3a CM .50 % volume", "[Day 1: Seeding of Hek 293 STF cells] Start with one almost... |
65,633 | Development of a simple and versatile in vitro method for production, stimulation, and analysis of bioengineered muscle | 4 | dx.doi.org/10.17504/protocols.io.ccb9ssr6 | https://www.protocols.io/view/development-of-a-simple-and-versatile-in-vitro-met-ccb9ssr6 | Karen Wells-Cembrano, Júlia Sala, Jose Antonio del Rio | TITLE: Development of a simple and versatile in vitro method for production, stimulation, and analysis of bioengineered muscle
AUTHORS: Karen Wells-Cembrano, Júlia Sala, Jose Antonio del Rio
[DESCRIPTION]
In recent years, 3D in vitro modeling of human skeletal muscle has emerged as a subject of increasing interest, du... | ["[Making muscle culture devices] Mark a line of 1.2 cm in the center of each 35 mm ∅ Petri dish. In order to obtain consistently centered markings, it is recommended to make a small stencil out of paper or plastic (Figure 2).", "[Preparation of cells and materials] Defrost myoblast cell line in a T75 flask and culture... |
49,548 | SARS-CoV2 GISAID submission protocol | 1 | dx.doi.org/10.17504/protocols.io.bumknu4w | https://www.protocols.io/view/sars-cov2-gisaid-submission-protocol-bumknu4w | Nabil-Fareed Alikhan, Emma Griffiths, Ruth Timme, Duncan MacCannell | TITLE: SARS-CoV2 GISAID submission protocol
AUTHORS: Nabil-Fareed Alikhan, Emma Griffiths, Ruth Timme, Duncan MacCannell
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol provides the steps needed to establish a new GISAID submission environment for your laboratory. Once established, th... | ["[GISAID Submissions]\nGISAID submissions require contextual information (metadata) and the COVID-19 consensus sequence or genome sequence. It is much simpler than other databases, as it has no hierachical structure. In GISAID, one set of contextual information links to one genome assembly.We advise that contextual in... |
63,523 | https://www.facebook.com/Condor-CBD-Gummies-104105128992798 | 1 | dx.doi.org/10.17504/protocols.io.eq2lyn2jrvx9/v1 | https://www.protocols.io/view/https-www-facebook-com-condor-cbd-gummies-10410512-caabsaan | Condor-CBD-Gummies | TITLE: https://www.facebook.com/Condor-CBD-Gummies-104105128992798
AUTHORS: Condor-CBD-Gummies
[DESCRIPTION]
to assist you with recuperating quickly and regularly with the top promoting hemp oil confine! This uncommon color utilizes 100% regular dynamic fixings just as NO THC to ensure that you get your preeminent rec... | ["Condor CBD Gummies\n\n \n\n\n➢Product Name —Condor CBD Gummies Reviews\n➢Main Benefits—Improve Metabolism & Help in Pain Relief\n➢Composition —NaturalOrganic Compound\n➢Side-Effects—NA\n➢Rating :—⭐⭐⭐⭐⭐\n➢Availability —Online\n➢Price (for Sale) Buy Now Here —Click Here\nCondor CBD Gummies - You plan to recuperate just... |
44,740 | Geometric Method for Estimating Coral Surface Area Using Image Analysis | 1 | dx.doi.org/10.17504/protocols.io.bpxcmpiw | https://www.protocols.io/view/geometric-method-for-estimating-coral-surface-area-bpxcmpiw | Rowan Mclachlan, Andrea Grottoli | TITLE: Geometric Method for Estimating Coral Surface Area Using Image Analysis
AUTHORS: Rowan Mclachlan, Andrea Grottoli
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol outlines a non-destructive geometric method for estimating the surface area of Scleractinian coral samples with relat... | ["[Photographing corals]\nPrepare the photograph area. Setup a tripod so that the digital camera is pointing downward, parallel to the workbench as shown in Fig. 1. Place a metric ruler on two blocks of equal height (at least two inches) and make sure a section of the ruler is visible in the digital camera field of vie... |
66,323 | Cannaleafz CBD Gummies Canada Reviews: Shark Tank Ingredients & Price on Website | 3 | dx.doi.org/10.17504/protocols.io.eq2lynrpevx9/v1 | https://www.protocols.io/view/cannaleafz-cbd-gummies-canada-reviews-shark-tank-i-ccztsx6n | Cannaleafz CBD Gummies | TITLE: Cannaleafz CBD Gummies Canada Reviews: Shark Tank Ingredients & Price on Website
AUTHORS: Cannaleafz CBD Gummies
[DESCRIPTION]
Cannaleafz CBD Gummies Reviews:CBD is generally utilized in different nations of the world for restorative purposes. Be that as it may, many individuals get high in the wake of con... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.estbeen | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>PBCV-1 is a large plaque virus (approximately 2-3 mm in diameter). The plaque size of the viruses reflects the length of the virus replication cycle. PBCV-1 has a life cycle of about 6-8 hours. When titering you should use plates that were poured no more than 1 day before ... | [] |
82,033 | Transferring C. elegans to S-basal to grow them in liquid culture | 4 | dx.doi.org/10.17504/protocols.io.81wgbyj41vpk/v1 | https://www.protocols.io/view/transferring-c-elegans-to-s-basal-to-grow-them-in-cucrwsv6 | Muhammad Zaka Asif, Man Shah, Yosef Smadi | TITLE: Transferring C. elegans to S-basal to grow them in liquid culture
AUTHORS: Muhammad Zaka Asif, Man Shah, Yosef Smadi
[DESCRIPTION]
This protocol describes day 2 of our workflow to grow worms in liquid culture to induce the production of natural products (in this case, 1-HP derivatives). In this protocol, we get... | ["Remove flasks with L1-arrested worms from the shaker", "Swirl vigorously for at least 30 seconds, then collect a 1 uL aliquot and place onto a glass slide for inspection. Use a 0.5 uL aliquot if there are more than 100 worms in the 1 uL aliquot.", "Count using a tally counter. Repeat for at least 3 trials (may need t... |
null | null | null | dx.doi.org/10.17504/protocols.io.mk5c4y6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>In this protocol,<span lang="en"><span class=""> the extraction of template DNA out of <em>Synechocystis</em> sp. PCC 6803 samples, the PCR of the template and the application on a agarosegel are described. The protocol was handed over by Maximilian Dietsch MSc.<br /></span><... | [] |
66,983 | Ice House Portable AC | 3 | dx.doi.org/10.17504/protocols.io.n92ldzdmov5b/v1 | https://www.protocols.io/view/ice-house-portable-ac-cdnfs5bn | hggsasd | TITLE: Ice House Portable AC
AUTHORS: hggsasd
[DESCRIPTION]
Ice House Portable AC
[STEPS] | [] |
103,473 | Nuclei Isolation from Human Subchondral Bone for 10x Multiome | 0 | dx.doi.org/10.17504/protocols.io.j8nlk8mrdl5r/v1 | https://www.protocols.io/view/nuclei-isolation-from-human-subchondral-bone-for-1-dhar32d6 | Takuya Sakamoto, Merissa Olmer, Martin Lotz | TITLE: Nuclei Isolation from Human Subchondral Bone for 10x Multiome
AUTHORS: Takuya Sakamoto, Merissa Olmer, Martin Lotz
[DESCRIPTION]
This protocol describes isolation of nuclei from fresh-frozen human knee subchondral bone for use in Omics analyses, including RNA-sequencing of ATAC-sequencing. Tissue dissociation w... | [] |
64,826 | Keto Complete ( Fraud Attention 2022 ) - Do Really Work Keto Complete Australia? | 3 | dx.doi.org/10.17504/protocols.io.rm7vzy918lx1/v1 | https://www.protocols.io/view/keto-complete-fraud-attention-2022-do-really-work-cbi2skge | jackweyand | TITLE: Keto Complete ( Fraud Attention 2022 ) - Do Really Work Keto Complete Australia?
AUTHORS: jackweyand
[DESCRIPTION]
Keto Complete Australia
[STEPS] | [] |
106,299 | SEQUENCING Protocol Template | 1 | null | https://www.protocols.io/view/sequencing-protocol-template-dj234qgn | Kathleen Pitz, Raïssa Meyer | TITLE: SEQUENCING Protocol Template
AUTHORS: Kathleen Pitz, Raïssa Meyer
[DESCRIPTION]
A protocol template created through the BeBOP project for sequencing.
Main protocol steps begin below the "Standard Operating Procedure" section. A detailed description of the BeBOP project and these templates is available here: ht... | ["[MIOP: Minimum Information about an Omics Protocol] MIOP Term\nValue\n \nmethodology category\n\n \nproject\n\n \npurpose\n\n \nanalyses\n\n \ngeographic location\n\n \nbroad-scale environmental context\n\n \nlocal environmental context\n\n \nenvironmental medium\n\n \ntarget\n\n \ncreator\n\n \nmaterials required\... |
80,834 | Laboratory and radiological data collection | 1 | dx.doi.org/10.17504/protocols.io.6qpvr459ogmk/v1 | https://www.protocols.io/view/laboratory-and-radiological-data-collection-cs7awhie | Kajiru Gad Kilonzo, Stefanie J. Krauth, Jo Halliday, Clive Kelly, Stefan Siebert, Gloria Temu, Christopher Bunn, Nateiya M Yongolo, Sally Wyke, Emma McIntosh, Blandina Mmbaga | TITLE: Laboratory and radiological data collection
AUTHORS: Kajiru Gad Kilonzo, Stefanie J. Krauth, Jo Halliday, Clive Kelly, Stefan Siebert, Gloria Temu, Christopher Bunn, Nateiya M Yongolo, Sally Wyke, Emma McIntosh, Blandina Mmbaga
[DESCRIPTION]
This protocol details laboratory and radiological data collection.
[S... | ["[Laboratory and radiological data collection] Following completion of the screening and examinations within both the community and hospital data capture, adult patients with evidence of synovitis or joint swelling will have 20 mL blood taken for later analysis of acute phase reactants (ESR, CRP) and autoantibodies (R... |
null | null | null | dx.doi.org/10.17504/protocols.io.dxb7im | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?. | [] |
60,213 | eDNA extraction with kaneka | 1 | dx.doi.org/10.17504/protocols.io.dm6gpb9xplzp/v1 | https://www.protocols.io/view/edna-extraction-with-kaneka-b62vrge6 | Fei Xia | TITLE: eDNA extraction with kaneka
AUTHORS: Fei Xia
[DESCRIPTION]
We used the Kaneka Simple DNA Extraction Kit version 2 (Kaneka) for on-site DNA extraction from Sterivex. We injected 500 µl of Solution A of the kit into the Sterivex and shook it by hand for 1 min. We collected the Solution A buffer in the Sterivex b... | ["Inject 250 µL of A buffer into the Sterivex, 1 min shake and rotate.", "Put Sterivex into 50ml tube and secured for centrifugation 5000 rpm, 5 min", "Add 40 µL of B buffer to the 1.5ml tube.", "Store the samples at -20 °C .", "Remove the liquid from the inlet and put into the 1.5ml tube.", "Mix by vortexing."] |
59,225 | Small Object and Artefact Photography - 'SOAP' Protocol | 5 | dx.doi.org/10.17504/protocols.io.b53zq8p6 | https://www.protocols.io/view/small-object-and-artefact-photography-39-soap-39-p-b53zq8p6 | Jacopo Niccolo Cerasoni, Felipe Do Nascimento Rodrigues | TITLE: Small Object and Artefact Photography - 'SOAP' Protocol
AUTHORS: Jacopo Niccolo Cerasoni, Felipe Do Nascimento Rodrigues
[DESCRIPTION]
Photography is among one of the most widely used methods in scientific publications to efficiently and objectively communicate morphological, technological and aesthet... | ["[Collect Equipment] Collect and prepare equipment (see materials section for full list of equipment).", "[Collect Equipment] Arguably the most important part of a camera is the lens. Before continuing with this method, we suggest the reader understand what type of lenses exist and which one works best for its use. In... |
63,158 | Keto Blast Gummies Reviews & Shark Tank 2022 | 1 | dx.doi.org/10.17504/protocols.io.6qpvr67q2vmk/v1 | https://www.protocols.io/view/keto-blast-gummies-reviews-amp-shark-tank-2022-b9wwr7fe | ketoblastiesreviews | TITLE: Keto Blast Gummies Reviews & Shark Tank 2022
AUTHORS: ketoblastiesreviews
[DESCRIPTION]
Keto Blast Gummies Reviews & Shark Tank 2022
[STEPS]
1. Keto Blast Gummies Reviews & Shark Tank 2022
Kwazi Keto Gummies & Keto Blast Gummies As we know that weight loss is a lengthy process. It isn't possible to lose... | ["Keto Blast Gummies Reviews & Shark Tank 2022\nKwazi Keto Gummies & Keto Blast Gummies As we know that weight loss is a lengthy process. It isn't possible to lose a lot of weight in one day. Numerous people tried their stylish to get into the right shape but they didn’t succeed. On the other hand, exercise and diet pl... |
28,348 | Naegleria gruberi plasmids | null | dx.doi.org/10.17504/protocols.io.7w4hpgw | https://www.protocols.io/view/naegleria-gruberi-plasmids-7w4hpgw | Anastasios Tsaousis, Eleana Kazana, Tobias von der Haar | TITLE: Naegleria gruberi plasmids
AUTHORS: Anastasios Tsaousis, Eleana Kazana, Tobias von der Haar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Maps and nucleotide sequences of the Naegleria gruberi transfection plasmids (pNaeg-HYG and pNaeg-NEO) reported in Faktorová D. et al. </div></div>
[STE... | [] |
71,066 | Sample preconditioning before scanning electron microscopy (SEM) | 4 | dx.doi.org/10.17504/protocols.io.36wgqj465vk5/v1 | https://www.protocols.io/view/sample-preconditioning-before-scanning-electron-mi-chm2t48e | An.Huang | TITLE: Sample preconditioning before scanning electron microscopy (SEM)
AUTHORS: An.Huang
[DESCRIPTION]
Bacterial samples need to be preconditioned before visualized by scanning electron microscope (SEM). This protocol describes preconditioning methods of bacteria before SEM.
[STEPS]
SECTION: Bacterial samples precon... | ["[Bacterial samples preconditioning] Obtain a small quantity of bacteria to be tested from a culture.", "[Bacterial samples preconditioning] Centrifuge the samples at5000 rpm, 10 min, 4 °C . Resuspend the pellet using 1X phosphate buffed saline (PBS). Repeat this step for three times. Keep the supernatant after the th... |
29,947 | High molecular weight DNA extraction from fungal tissue for the long read PacBio sequencing | null | dx.doi.org/10.17504/protocols.io.9g3h3yn | null | Reynaldi Darma, Megan McDonald | TITLE: High molecular weight DNA extraction from fungal tissue for the long read PacBio sequencing
AUTHORS: Reynaldi Darma, Megan McDonald
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Obtaining high molecular weight DNA from certain materials for PacBio sequencing can be quite challenging because... | ["[Grinding the spores]\nGrind fungal tissues 3-4 times for 10-15 sec bursts adding liquid nitrogen each time. Do not exert excessive pressure while grinding the spores as this may shear the DNA.", "[DNA extraction]\nAdd 200 µl proteinase Proteinase K (20 mg/mL) and incubate at RT for 1.5 hours on the orbital shaker wi... |
98,034 | FUNDIS version ONT dA-tailing for Fungal Barcoding | 4 | null | https://www.protocols.io/view/fundis-version-ont-da-tailing-for-fungal-barcoding-dbys2pwe | Stephen Douglas Russell, Harte Singer | TITLE: FUNDIS version ONT dA-tailing for Fungal Barcoding
AUTHORS: Stephen Douglas Russell, Harte Singer
[DESCRIPTION]
This protocol is for dA-tailing, which is an enzymatic method for adding a non-templated nucleotide to the 3' end of a blunt, double-stranded DNA molecule. In other words, this puts A-chains on the en... | ["[End repair/A-tailing] Put a 1.5mL aliquot of fresh molecular water on a heat block at 55 °C. This will be used after the cleanup step towards the end of this protocol.\n\nTurn on your PCR thermal cycler so that the heated lid begins to come up to temp.\n\nTake out the materials for library prep from the freezer. Fli... |
88,107 | Ethnic-Racial Socialization in Early Childhood: Scoping Review Protocol | 1 | dx.doi.org/10.17504/protocols.io.q26g7pmq8gwz/v1 | https://www.protocols.io/view/ethnic-racial-socialization-in-early-childhood-sco-c2ajyacn | Erica E Coates, Charles S. Dorris MLIS, Alison B McLeod, Kaela Farrise | TITLE: Ethnic-Racial Socialization in Early Childhood: Scoping Review Protocol
AUTHORS: Erica E Coates, Charles S. Dorris MLIS, Alison B McLeod, Kaela Farrise
[DESCRIPTION]
Objective: This scoping review aims to assess the state of the literature on ethnic-racial socialization (ERS) during the early childhood period. ... | ["[Introduction] Caregivers use ethnic-racial socialization (ERS) to transmit information about race and ethnicity to their children (Hughes et al., 2006). Research on ERS has increased over the past 20 years but has mostly focused on ERS with older children and adolescents (see Umaña‐Taylor & Hill, 2020 for a review).... |
71,152 | Manual Blood Pressure (Auscultation Technique) | 1 | dx.doi.org/10.17504/protocols.io.5jyl8j568g2w/v1 | https://www.protocols.click/view/manual-blood-pressure-auscultation-technique-chqqt5vw | g.spencer | TITLE: Manual Blood Pressure (Auscultation Technique)
AUTHORS: g.spencer
[DESCRIPTION]
The following SOPs outline how to take a blood pressure measurement both manually and using an automated device.
Blood-pressure measurement is warranted in any situation that requires assessment of cardiovascular health, including ... | ["[Equipment Required] Stethoscope\nSphygmomanometer (cuff, bulb and a manometer)", "[Preparing for the Measurement] Follow the Steps below to help prepare your PPC for measurement:\n\nThe patient’s back and legs should be supported with legs uncrossed and feet resting on a firm surface\n\nThe patient’s arms should be ... |
null | null | null | dx.doi.org/10.17504/protocols.io.iqqcdvw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Forms used for data collection in the study entitled: “Risk factors for adverse drug reactions in pediatric inpatients: a cohort study”</p>
<p> </p>
<p>Forms 1 and 2 must be used in the first pharmaceutical consultation after hospital admission of the patient:</p>
<p>i) form ... | [] |
56,735 | spyDNA650 staining with CO2 independent media for live endothelium microscopy | 4 | null | https://www.protocols.io/view/spydna650-staining-with-co2-independent-media-for-b3m7qk9n | Emir Bora Akmeriç | TITLE: spyDNA650 staining with CO2 independent media for live endothelium microscopy
AUTHORS: Emir Bora Akmeriç
[DESCRIPTION]
"spyDNA650 staining with CO2 independent media for live endothelium microscopy"
[STEPS]
SECTION: Staining
1. Dilute 1:1000 SPY650 DNA in CO2 independent media(500 uL total volume for 2 slide... | ["[Staining] Dilute 1:1000 SPY650 DNA in CO2 independent media(500 uL total volume for 2 slides, 220 uL per slide and incubate in incubator for 20 mins", "[Staining] Gently remove overnight media from the u-slides and add warmed dye-media mix. Check whether monolayer is preserved. incubate for 2-3 hours", "[Flow Unit E... |
73,157 | Adquisición Desideratas Biblioteca UCAM | 1 | dx.doi.org/10.17504/protocols.io.81wgbyzdyvpk/v1 | https://www.protocols.io/view/adquisici-n-desideratas-biblioteca-ucam-cjpdumi6 | Antonio Rex Alegria | TITLE: Adquisición Desideratas Biblioteca UCAM
AUTHORS: Antonio Rex Alegria
[DESCRIPTION]
Procedimiento para la Adquisición de material bibliográfico
[STEPS]
1. Ve a la página web de la Biblioteca de la UCAM https://www.ucam.edu/biblioteca
2. Pulsa en el icono de Adquisiciones
3. Se abre una nueva pantalla... | ["Ve a la página web de la Biblioteca de la UCAM https://www.ucam.edu/biblioteca", "Pulsa en el icono de Adquisiciones", "Se abre una nueva pantalla.", "Seleccionamos el icono de Solicitud de Adquisiciones/Soporte.", "Nos identificamos con nuestro usuario y contraseña.", "Introducimos los datos que nos solicita el sop... |
61,814 | LDM protocol for estimating plasmid conjugation rates | 4 | null | https://www.protocols.io/view/ldm-protocol-for-estimating-plasmid-conjugation-ra-b8kwruxe | Olivia Kosterlitz, Claire Wate | TITLE: LDM protocol for estimating plasmid conjugation rates
AUTHORS: Olivia Kosterlitz, Claire Wate
[DESCRIPTION]
This is a detailed protocol to implement the LDM approach for estimating plasmid conjugation rates. To get a conceptual overview of the approach, please read the accompanying manuscript:
The protoco... | ["[Phase 1: Minimum inhibitory concentration assay with the transconjugant-selecting medium. medium.] Prepare bacterial cultures.\n\n Start cultures from freezer stocks of the donors, recipients, and transconjugants. Supplement the donor and transconjugant growth culture medium with the appropriate selection to maintai... |
74,910 | iPad_Lidar_surface_roughness_processing | 5 | dx.doi.org/10.17504/protocols.io.6qpvr4pe3gmk/v1 | https://www.protocols.io/view/ipad-lidar-surface-roughness-processing-cmd6u29e | Alex White | TITLE: iPad_Lidar_surface_roughness_processing
AUTHORS: Alex White
[DESCRIPTION]
This protocol was used to process LIDAR data acquired over 3-m transects of forest floor, using an Apple iPad Pro 2022 app entitled 3D Scanner App in “LIDAR Advanced” mode set to the smallest possible maximum depth (30 cm) and resolution ... | ["Open CloudCompare.", "Open textured output file:", "Select the file in the DB Tree on the left. \n \n A bounding box will appear around the mesh.", "Click the Cross Section icon in the toolbar.", "Click the folder icon.", "Navigate to a transect folder (e.g. MA404-1-EW_20_02_48) in Box > SMAPVEX22_data > MA_lid... |
null | null | null | dx.doi.org/10.17504/protocols.io.kq9cvz6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The appropriate use of clinically accurate diagnostic tests is essential for the detection of pertussis, a poorly controlled vaccine-preventable disease. The purpose of this study was to estimate the sensitivity and specificity of different diagnostic criteria including cultu... | [] |
44,323 | Quantitate Library | 4 | dx.doi.org/10.17504/protocols.io.bpibmkan | https://www.protocols.io/view/quantitate-library-bpibmkan | Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo | TITLE: Quantitate Library
AUTHORS: Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Profiling of RNA binding protein targets in vivo provides critical insights int... | ["Quantitate using D1000 DNA Tapes for the Agilent TapeStation."] |
76,026 | RCA-NGS for RNA viruses | 4 | dx.doi.org/10.17504/protocols.io.rm7vzb7wrvx1/v1 | https://www.protocols.io/view/rca-ngs-for-rna-viruses-cng2vbye | Masayasu Misu, Tomoki Yoshikawa, Satoko Sugimoto, Yuki Takamatsu, Takeshi Kurosu, Yukiteru Ouji, Masahide Yoshikawa, Masayuki Shimojima, Hideki Ebihara, Masayuki Saijo | TITLE: RCA-NGS for RNA viruses
AUTHORS: Masayasu Misu, Tomoki Yoshikawa, Satoko Sugimoto, Yuki Takamatsu, Takeshi Kurosu, Yukiteru Ouji, Masahide Yoshikawa, Masayuki Shimojima, Hideki Ebihara, Masayuki Saijo
[DESCRIPTION]
This RCA-NGS were optimized for an NGS machine, MinION. These methods do not require nucleic acid... | ["[Preparation for virus supernatant] Centrifuge the working stock virus to remove debris.\n 6000 x g, 10 min", "[Preparation for virus supernatant] Unwanted DNA and RNA mainly originating from the virus-infected cells are digested using .", "[The viral genomic RNA extraction] The viral genomic RNA extraction is perfo... |
22,292 | AFM tip functionalization with glutaraldehyde | null | dx.doi.org/10.17504/protocols.io.zzuf76w | null | George Lykotrafitis | TITLE: AFM tip functionalization with glutaraldehyde
AUTHORS: George Lykotrafitis
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Attach ligand protein to the AFM cantilever tip through functionalization. Further use it in the single molecule force spectroscopy to detect corresponding receptors on t... | ["Silanize the tip with 2% v/v 3-aminopropyltiethoxysilane (APTES) in acetone for 10 min at 25°C.", "Rinse the tip with deionized (DI) water.", "Immerse the tip in 0.5% v/v glutaraldehyde in DI water for 30 min at 25°C.", "Rinse the tip with DI water.", "Incubate the tip in protein solution (100-300 µg/mL, eg. 100µg/mL... |
null | null | null | dx.doi.org/10.17504/protocols.io.cq9vz5 | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?.
?.
?.
?. | [] |
45,785 | A New Absolute Quantification Method for Protein | 4 | null | https://www.protocols.io/view/a-new-absolute-quantification-method-for-protein-bqxzmxp6 | 181830691 | TITLE: A New Absolute Quantification Method for Protein
AUTHORS: 181830691
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Absolute quantification method is essential for biological research. For absolute quantification, it is usually necessary to draw standard curves according to molecules w... | [] |
84,558 | Protocol for Identifying Highly Pathogenic Salmonella Using the HPS Multiplex PCR Assay | 4 | dx.doi.org/10.17504/protocols.io.6qpvr3on3vmk/v1 | https://www.protocols.io/view/protocol-for-identifying-highly-pathogenic-salmone-cwtnxeme | Dayna Harhay, Kerry D. Brader, Jim Bono, Gregory P Harhay, Mick Bosilevac, Tatum S Katz | TITLE: Protocol for Identifying Highly Pathogenic Salmonella Using the HPS Multiplex PCR Assay
AUTHORS: Dayna Harhay, Kerry D. Brader, Jim Bono, Gregory P Harhay, Mick Bosilevac, Tatum S Katz
[DESCRIPTION]
This protocol describes how to perform a multiplex PCR assay for the identification of Highly Pathogenic Salmonel... | ["[Bacterial Lysis (BAX lysis) Procedure to prepare DNA template] Reaction: \nAdd 100 µL Protease/Lysis Buffer per well in the PCR plate\nAdd 2.5 µL Sample (overnight culture) per well", "[Bacterial Lysis (BAX lysis) Procedure to prepare DNA template] In a PCR plate, dispense 100 µL of the BAX lysis buffer per well, ad... |
24,047 | Biochemical Measures of Neuropathy - GSSG (Non-Enzymatic) | null | dx.doi.org/10.17504/protocols.io.3qpgmvn | null | Eva Feldman | TITLE: Biochemical Measures of Neuropathy - GSSG (Non-Enzymatic)
AUTHORS: Eva Feldman
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">Oxidative stress is highly correlated with the metabolic changes caused by hyperglyce... | ["[Sample Preparation:]\nPrior to dissection, perfuse tissue with PBS pH7.4 with 0.16 mg/ml heparin.1. If using extract from GSH add 100 μl 0.04 M N-ethylmaleimide to 200 μl homogenate then skip to Performing the Assay or2. Label 3 sets 1.5 ml and 1 set of 0.6 ml micro centrifuge tubes.3. Prepare 3 M HClO4 and freeze.... |
91,086 | Synthesis of Carbon Dots using Citric acid (Solvent Free reaction) | 6 | dx.doi.org/10.17504/protocols.io.e6nvwd25dlmk/v1 | https://www.protocols.io/view/synthesis-of-carbon-dots-using-citric-acid-solvent-c47nyzme | qurashiubaid | TITLE: Synthesis of Carbon Dots using Citric acid (Solvent Free reaction)
AUTHORS: qurashiubaid
[DESCRIPTION]
Carbon Dots are the fluorescent nanoparticles that have wide range of applications. Facile synthesis of Carbon Dots paves the way for making this material mainstream for use. Synthesis of Carbon Dots by using ... | ["[Synthesis of Carbon Dots using Citric acid (Solvent Free reaction)] 2 g 120 °C", "[Synthesis of Carbon Dots using Citric acid (Solvent Free reaction)] Once you get the yellow molten compound , drop it in the 0.1 Mass Percent 100ml solution, dropwise and stir it on magnetic stirrer.", "[Synthesis of Carbon Dots usi... |
98,185 | Single-cell RNA-seq | 0 | null | https://www.protocols.io/view/single-cell-rna-seq-db5h2q36 | Siwei Li | TITLE: Single-cell RNA-seq
AUTHORS: Siwei Li
[DESCRIPTION]
Single-cell RNA-seq
[STEPS]
SECTION: Single-cell RNA-seq
1. Tissues were transferred to sterile culture dishes with 10 ml of 1× Dulbecco's phosphate-buffered saline (DPBS; Thermo Fisher, Cat. no. 14190144) on ice to remove residual tissue storage solution and... | ["[Single-cell RNA-seq] Tissues were transferred to sterile culture dishes with 10 ml of 1× Dulbecco's phosphate-buffered saline (DPBS; Thermo Fisher, Cat. no. 14190144) on ice to remove residual tissue storage solution and then minced on ice. We used the dissociation enzyme 0.25% trypsin (Thermo Fisher, Cat. no. 25200... |
47,843 | Protocol for SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing.v3.Neb | 4 | dx.doi.org/10.17504/protocols.io.bsybnfsn | https://www.protocols.io/view/protocol-for-scv-2000bp-a-primer-panel-for-sars-co-bsybnfsn | Valeriia Kaptelova, Speranskaya AS, et.al | TITLE: Protocol for SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing.v3.Neb
AUTHORS: Valeriia Kaptelova, Speranskaya AS, et.al
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Here we present a new primer panel that allows amplifying the complete genome of SARS-CoV-2 (the causative vi... | ["[1. cDNA]\nRNA samples should be stored at -80 °C and thawed on ice.NGS library preparation and sequencing:cDNAReverse transcription reaction was performed using 10 μL of the RNA samples, random hexanucleotide primers, and Reverta-L kit (AmpliSens, Russia) according to the manufacturer’s instructions. Prepare a ready... |
null | null | null | dx.doi.org/10.17504/protocols.io.meyc3fw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This study aimed to investigate the effects of lactic acid bacterial fermentation extract (LABEF) on cystitis induced by <em>Escherichia coli</em> lipopolysaccharide (LPS) in the mouse bladder by pathological analyses and measurement of the levels of tumor necrosis factor-alp... | [] |
57,744 | Freezing of hPSCs grown on MEFs | 4 | dx.doi.org/10.17504/protocols.io.b4mqqu5w | https://www.protocols.io/view/freezing-of-hpscs-grown-on-mefs-b4mqqu5w | Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner | TITLE: Freezing of hPSCs grown on MEFs
AUTHORS: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner
[DESCRIPTION]
This protocol describes the standard procedure of freezing human pluripotent stem cells (hPSCs), which were grown on inactivated mouse embryonic fibroblasts (MEFs).
General notes
1. T... | ["[A. Freezing of hPSCs as single cell solution using trypsin] When hPSCs reach 50% confluency (usually on day 6 from last passage), change medium to 3 ml hPSCs medium + Rock inhibitor, to prepare for freezing the next day.", "[A. Freezing of hPSCs as single cell solution using trypsin] Before starting:\na. Prepare Fre... |
96,667 | Protocol: Neurophysiological Effects of Craniosacral Treatment on Heart Rate Variability | 0 | dx.doi.org/10.17504/protocols.io.4r3l22xjjl1y/v1 | https://www.protocols.io/view/protocol-neurophysiological-effects-of-craniosacra-dam32c8n | Andrew C Cook, Anna Egli, Nathan E Cohen, Sunni Coyne, Min Chae, Brandon Kapalko, Kyrie Bernardi, Randy Scott DO | TITLE: Protocol: Neurophysiological Effects of Craniosacral Treatment on Heart Rate Variability
AUTHORS: Andrew C Cook, Anna Egli, Nathan E Cohen, Sunni Coyne, Min Chae, Brandon Kapalko, Kyrie Bernardi, Randy Scott DO
[DESCRIPTION]
Craniosacral treatment is an osteopathic technique grounded in the assumption that ther... | ["[Title] Neurophysiological Effects of Craniosacral Treatment on Heart Rate Variability", "[Registration] Protocols.io", "[Authors] Andrew C. Cook: Lake Erie College of Osteopathic Medicine - Bradenton; Bradenton, FL\nACook28563@med.lecom.edu \nORCID: https://orcid.org/0000-0001-6332-1993\n\nAnna Egli: Lake Erie Colle... |
89,791 | The Bcc qPCR NAD assay for the specific rapid quantitative detection of all Bcc species | 1 | dx.doi.org/10.17504/protocols.io.4r3l223wql1y/v1 | https://www.protocols.io/view/the-bcc-qpcr-nad-assay-for-the-specific-rapid-quan-c3w7yphn | Huong Thu Duong, Shannon Fullbrook, Kate Reddington, Elizabeth Minogue, Thomas Barry | TITLE: The Bcc qPCR NAD assay for the specific rapid quantitative detection of all Bcc species
AUTHORS: Huong Thu Duong, Shannon Fullbrook, Kate Reddington, Elizabeth Minogue, Thomas Barry
[DESCRIPTION]
The Bcc qPCR NAD assay presented is an internally controlled duplex assay (incorporating an IAC), targeting a region... | ["[Prepare a qPCR master mix (in a DNA-free preparation hood)] Thaw qPCR reagents and samples on the bench, flick to mix and briefly spin down on a tabletop centrifuge.", "[The Bcc qPCR NAD assay] Transfer the plate into the thermal cycler after setting it up appropriately and carry out qPCR reaction at the following c... |
57,727 | Quantitative detection of vitamin B12 in algae by bioassay and ICP-MS/MS | 4 | dx.doi.org/10.17504/protocols.io.14egn7726v5d/v1 | https://www.protocols.io/view/quantitative-detection-of-vitamin-b12-in-algae-by-b4k7quzn | Sunnyjoy Dupuis, Stefan Schmollinger, Sabeeha S. Merchant | TITLE: Quantitative detection of vitamin B12 in algae by bioassay and ICP-MS/MS
AUTHORS: Sunnyjoy Dupuis, Stefan Schmollinger, Sabeeha S. Merchant
[DESCRIPTION]
This protocol describes two methods for determining the amount of vitamin B12 present in the spent medium and cell lysate of algae cultures. The first meth... | ["[E. coli B12 Bioassay]", "[E. coli B12 Bioassay] Prepare cell culture fractions:", "[E. coli B12 Bioassay] Collect 2 mL culture into a 2 ml screw-cap tube.", "[E. coli B12 Bioassay] Centrifuge at >8000 rcf, 2 min. Quickly transfer 950 µL of the spent medium supernatant to each of two 1.5 ml screw-cap tubes without ... |
85,883 | Mounting and Coverslipping Mouse Brain Sections | 1 | dx.doi.org/10.17504/protocols.io.n92ldmpy7l5b/v1 | https://www.protocols.io/view/mounting-and-coverslipping-mouse-brain-sections-cx43xqyn | Naveen Ouellette, daphne.toglia, Holly Myers | TITLE: Mounting and Coverslipping Mouse Brain Sections
AUTHORS: Naveen Ouellette, daphne.toglia, Holly Myers
[DESCRIPTION]
This protocol details mounting thin sliced mouse brain tissue sections onto charged slides in a uniform orientation that does not create wrinkles or artifacts that might interfere with imaging, an... | ["[Mounting] Place a drop of 1xPBS onto a 1\"x3\" slide.", "[Mounting] Using a small paintbrush, lift the first tissue section to be mounted out of the storage well plate and place section into the petri dish so that it lays flat close to the surface of the 1xPBS & Triton X-100 0.2% solution.", "[Mounting] Repeat steps... |
108,826 | Crystallisation of Enterovirus D68 3C protease | 1 | dx.doi.org/10.17504/protocols.io.rm7vzjp45lx1/v1 | https://www.protocols.io/view/crystallisation-of-enterovirus-d68-3c-protease-dnh25b8e | ryan Lithgo, Peter Marples, Lizbé Koekemoer, Daren Fearon | TITLE: Crystallisation of Enterovirus D68 3C protease
AUTHORS: ryan Lithgo, Peter Marples, Lizbé Koekemoer, Daren Fearon
[DESCRIPTION]
The development of effective broad-spectrum antivirals forms an important part of preparing for future pandemics. A cause for concern is the currently emerging pathogen Enterovirus D68... | ["[Crystallisation experiment] Protein and buffer requirements:\n14.4 µL35 mg/mL \n2.88 mL \n7.2 µL seeds, dilution 1:1 000 000", "[Crystallisation experiment] Dispense 30 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.\nDispense 50 nL35 mg/mL to each lens using the SPT mosquito.\nDi... |
null | null | null | dx.doi.org/10.17504/protocols.io.hhab32e | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<div>
<div>
<div>
<p>This protocol is from 'Flower Ca<sup>2+</sup> channel in CME and ADBE' of Yao CK et al.</p>
<div>
<div>
<p> </p>
<p>Please see the manuscript for the full method details.</p>
</div>
</div>
</div>
</div>
</div>
[BEFORE_START]
<p>You'll need: </p>
<p> </p>
<p... | [] |
96,702 | Bacterial-induced neutrophilic nasal inflammation in mice | 0 | dx.doi.org/10.17504/protocols.io.rm7vzxwzxgx1/v1 | https://www.protocols.io/view/bacterial-induced-neutrophilic-nasal-inflammation-dan62dhe | Alba Sánchez Montalvo, Aaron Ziani Zeryouh, Marylène Lecocq, Charles Pilette, Valérie Hox | TITLE: Bacterial-induced neutrophilic nasal inflammation in mice
AUTHORS: Alba Sánchez Montalvo, Aaron Ziani Zeryouh, Marylène Lecocq, Charles Pilette, Valérie Hox
[DESCRIPTION]
The present surgical procedure provides a step-by-step and detailed protocol with all the critical information to develop a bacterial-induced... | ["[The day before surgery] Prepare the chosen bacteria", "[Surgical procedure] Shave the snout over the intervention area and fix the mouse on a sterile field (Figure 1, A).", "[The day before surgery] Prepare the nasal tampon", "[Pre-surgery] Weight the mouse and administer general anesthesia accordingly: a mix of Xyl... |
78,849 | Prueba2 | 4 | dx.doi.org/10.17504/protocols.io.j8nlkwyxdl5r/v1 | https://www.protocols.io/view/prueba2-cq89vzz6 | Yo | TITLE: Prueba2
AUTHORS: Yo
[DESCRIPTION]
Resuemem
[STEPS]
1. Saludar
2. Preguntar como estas?
3. Decir adios | ["Saludar", "Preguntar como estas?", "Decir adios"] |
106,016 | DNA Extraction from Swabbed Feces for Depositor Species Identification - 2024 | 0 | dx.doi.org/10.17504/protocols.io.5qpvokdn9l4o/v1 | https://www.protocols.io/view/dna-extraction-from-swabbed-feces-for-depositor-sp-djr84m9w | Karla Vargas | TITLE: DNA Extraction from Swabbed Feces for Depositor Species Identification - 2024
AUTHORS: Karla Vargas
[DESCRIPTION]
Protocol developed by the Culver Lab at the University of Arizona to extract DNA from feces for depositor species identification
[STEPS]
SECTION: DAY 1
2. Saturate cotton-tipped applicators with PB... | ["[DAY 1] Saturate cotton-tipped applicators with PBS buffer.\n● Keep cotton-tipped applicators in PBS buffer for at least 3 minutes.", "[DAY 1] Pipette 25 μl of proteinase K and 300 μl of ATL buffer (from DNeasy kit) into a new, labeled 2 ml microcentrifuge tube.", "[DAY 1] Place a sheet of copy paper on a stainless-s... |
85,305 | Immunostaining of iPSC-derived neurons for quantification of synaptic proteins | 4 | dx.doi.org/10.17504/protocols.io.8epv5x91ng1b/v1 | https://www.protocols.click/view/immunostaining-of-ipsc-derived-neurons-for-quantif-cxizxkf6 | Dan Dou, Erika Holzbaur | TITLE: Immunostaining of iPSC-derived neurons for quantification of synaptic proteins
AUTHORS: Dan Dou, Erika Holzbaur
[DESCRIPTION]
Here, we fix, permeabilize, and stain human iPSC-derived neurons for the purpose of observing and quantifying somal proteins of interest. For preceding culture of neurons, see "Protocol:... | ["At DIV14, fix human iNeurons in 4% paraformaldehyde supplemented with 4% sucrose for 15 minutes at 37 degrees C", "Wash four times with PBS", "Permeabilize for 15 minutes in 0.2% Triton-X in PBS", "Block for 1 hour with 5% goat serum and 1% BSA in PBS", "Incubate in primary antibody diluted in blocking solution at ro... |
49,458 | Processing a Viral Metagenome Using iVirus | 2 | dx.doi.org/10.17504/protocols.io.buisnuee | https://www.protocols.io/view/processing-a-viral-metagenome-using-ivirus-buisnuee | Benjamin Bolduc | TITLE: Processing a Viral Metagenome Using iVirus
AUTHORS: Benjamin Bolduc
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>A collection of protocols designed to guide the user in processing a viral metagenome from raw sequence data to assembly, and subsequent analysis. The user uses </span><sp... | [] |
60,062 | Staphilococcus Aureus Sampling | 4 | dx.doi.org/10.17504/protocols.io.81wgb6pk1lpk/v6 | https://www.protocols.io/view/staphilococcus-aureus-sampling-b6v6re9e | Pol Roca Cugat, Olga Sánchez | TITLE: Staphilococcus Aureus Sampling
AUTHORS: Pol Roca Cugat, Olga Sánchez
[DESCRIPTION]
This protocol is intended to study the affectation of Staphilococcus Aureus, including the MRSA variant. It outlines the basic protocol for a multi-subject study.
[GUIDELINES]
This protocol is intended to study the affectation... | ["[Preparation] Wash your hands with soap. Put on your lab coat, your mask and your goggles or face shield. Make sure your mask is airtight and air cannot escape through the sides.", "[Preparation] Prepare the area where you are going to work. Disinfect the surfaces with the bleach solution.\nThe subjects should not be... |
53,732 | Immunohistochemical labelling of lower urinary tract afferents in spinal cord | 1 | dx.doi.org/10.17504/protocols.io.byqcpvsw | https://www.protocols.io/view/immunohistochemical-labelling-of-lower-urinary-tra-byqcpvsw | Janet R Keast, Peregrine B Osborne, John-Paul Fuller-Jackson | TITLE: Immunohistochemical labelling of lower urinary tract afferents in spinal cord
AUTHORS: Janet R Keast, Peregrine B Osborne, John-Paul Fuller-Jackson
[DESCRIPTION]
This protocol is used for immunohistochemical visualisation of cholera toxin subunit B within afferents innervating the lower urinary tract in cryose... | ["[Preparation of cryosections] Cryoprotect fixed tissue (L5-S2 spinal cord) in phosphate-buffered saline (PBS; 0.1 M, pH7.2) containing 30% sucrose. This should be performed at 4C, 24-72h prior to cutting.", "[Preparation of cryosections] Embed tissue in cryomold using OCT, freeze in cryostat and cut sections (40 µm),... |
76,419 | Inoue transformation buffer | 4 | null | https://www.protocols.io/view/inoue-transformation-buffer-cnvbve2n | Andreas Sagen | TITLE: Inoue transformation buffer
AUTHORS: Andreas Sagen
[DESCRIPTION]
The Inoue method of transformation is a chemical transformation method of prokaryotes (bacteria), which add significant improvements to methods such as the classic Calcium chloride transformation method.
[STEPS]
SECTION: 10x Inoue transformation ... | ["[10x Inoue transformation buffer] Add 80 mL distilled water to a 100 mL tube", "[10x Inoue transformation buffer] Measure and add 10.89 g Manganese chloride, 2.2 g Calcium chloride, 3.0 g PIPES and 18.65 g Potassium chloride\n\nMaterials:", "[10x Inoue transformation buffer] Add distilled water to 100 mL", "[10x Inou... |
83,027 | Isolation and storage of PBMCs from human peripheral blood samples | 1 | dx.doi.org/10.17504/protocols.io.14egn3bzml5d/v1 | https://www.protocols.click/view/isolation-and-storage-of-pbmcs-from-human-peripher-cvbtw2nn | Yunge Zhao, Joseph Rabin, Joel Linden, Christine L Lau | TITLE: Isolation and storage of PBMCs from human peripheral blood samples
AUTHORS: Yunge Zhao, Joseph Rabin, Joel Linden, Christine L Lau
[DESCRIPTION]
This protocol descried detailed steps on how to isolate and store PBMCs from human peripheral blood samples. By following this protocol, you can isolate and store PBM... | ["[Isolation and storage of PBMCs from human peripheral blood samples] Collect 2 tubes of 10ml of blood in EDTA tubes and transport to the laboratory in an ice box.", "[Isolation and storage of PBMCs from human peripheral blood samples] Spin samples at 1600 rpm (2500 x G) for 10 minutes in collection tubes.", "[Isolati... |
51,771 | Protocol 1: Clinical and demographic information | 1 | dx.doi.org/10.17504/protocols.io.bws3pegn | https://www.protocols.io/view/protocol-1-clinical-and-demographic-information-bws3pegn | Sophie Adler, Mathilde Ripart, Meld Project, Konrad Wagstyl | TITLE: Protocol 1: Clinical and demographic information
AUTHORS: Sophie Adler, Mathilde Ripart, Meld Project, Konrad Wagstyl
[DESCRIPTION]
The MELD Project is an international collaboration aiming to create open-access, robust and generalisable tools for FCD detection.
This MRI data protocol details
1) How to ge... | ["[Access to the RedCap Database] Anonymised clinical and demographic information for all participants should be entered by filling in the participant information survey on Redcap.\n\nEach participating site needs to send us the institutional email addresses of the individuals who will be carrying out data entry. Accou... |
103,458 | RNA Isolation of Human Osteochondral, Subchondral Bone and Cartilage Tissues | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8m2zlmk/v1 | https://www.protocols.io/view/rna-isolation-of-human-osteochondral-subchondral-b-dhaa32ae | Ann-Marie Hageny, Merissa Olmer, Martin Lotz | TITLE: RNA Isolation of Human Osteochondral, Subchondral Bone and Cartilage Tissues
AUTHORS: Ann-Marie Hageny, Merissa Olmer, Martin Lotz
[DESCRIPTION]
This protocol demonstrates how to perform RNA isolation from fresh snapfrozen Human Osteochondral Tissue, Subchondral Bone and Cartilage tissues.
[STEPS]
SECTION: Ti... | ["[Tissue Homogenization] Precool Benchtop Centrifuge and make sure temperature is at 4℃.", "[Tissue Homogenization] Prepare tools, mortar and pestle, and work surface by spraying down with RNAse Away.", "[Tissue Homogenization] Weigh out 110-150mg of fresh or snapfrozen tissue. If you have to cut a smaller piece of sn... |
52,279 | Detection of Cryptosporidium in stool sample by PCR-RFLP | 4 | dx.doi.org/10.17504/protocols.io.bxaxpifn | https://www.protocols.io/view/detection-of-cryptosporidium-in-stool-sample-by-pc-bxaxpifn | botchiesenyo | TITLE: Detection of Cryptosporidium in stool sample by PCR-RFLP
AUTHORS: botchiesenyo
[DESCRIPTION]
Nested PCR-RFLP adapted from Nichols, R.A.B., Campbell, B.M. and Smith, H.V., 2003. Identification of Cryptosporidium spp. oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters by using a ... | ["[Nested PCR] Extract whole genomic DNA from stool samples. \n\nPositive control DNA for Cryptosporidium parvum may be obtained from ATCC. Alternatively you can extract genomic DNA from wild type Cryptosporidium parvum purchased from Bunchgrass Farms (Deary, Idaho, USA), Waterborne Inc (New Orleans, Louisiana, USA), o... |
70,236 | Application of PHYTO-PAM-II (Compact Version) on Aureococcus anophagefferens cultures for photosynthetic efficiency and quantum yield of PSII | 1 | dx.doi.org/10.17504/protocols.io.yxmvm2785g3p/v1 | https://www.protocols.io/view/application-of-phyto-pam-ii-compact-version-on-aur-cgt4twqw | Emily E. Chase, Gwen Stark, Alex Truchon, Steven W Wilhelm | TITLE: Application of PHYTO-PAM-II (Compact Version) on Aureococcus anophagefferens cultures for photosynthetic efficiency and quantum yield of PSII
AUTHORS: Emily E. Chase, Gwen Stark, Alex Truchon, Steven W Wilhelm
[DESCRIPTION]
A protocol used to acquire photosynthetic efficiency (Fv/Fm) and quantum yield of photo... | ["[SAMPLE PREPARATION] Prepare 3 mL of each culture of Aureococcus anophagefferens to be measured in appropriately labelled, separate 15 mL Falcon tubes. Additionally, prepare at least one sample to carry out the auto-gain function if you do not know the appropriate gain (see Step 3.1).", "[SAMPLE PREPARATION] For acqu... |
41,768 | Protocol to apply saliva samples to quantum mechanical tunneling current real-time point-of-care COVID-19 sensor. | 4 | dx.doi.org/10.17504/protocols.io.bk2gkybw | https://www.protocols.io/view/protocol-to-apply-saliva-samples-to-quantum-mechan-bk2gkybw | Massood Tabib-Azar | TITLE: Protocol to apply saliva samples to quantum mechanical tunneling current real-time point-of-care COVID-19 sensor.
AUTHORS: Massood Tabib-Azar
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to operate an electronic rapid (1 min) point-of-care SARS-CoV-2 sensor</div></div... | ["Apply a small amount (~1 uL) of saliva on the sensor element at room temperature.", "2. Wait for 30 s. The sensor sytem wil turn on a green LED to alert you at the end of the 30 s.", "Measure the sensor I-V using the paired smart phone APP or click on the \"Read\" botton on the sensor system.", "Apply 60 C water on t... |
null | null | null | dx.doi.org/10.17504/protocols.io.ieacbae | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p> </p>
<p>The protocol described here is an adaptation from this paper:</p>
<p> </p>
<p>Sun, Z. Z., Hayes, C. A., Shin, J., Caschera, F., Murray, R. M., Noireaux, V. Protocols for Implementing an <em>Escherichia coli</em> Based TX-TL Cell-Free Expression System for Synthetic B... | [] |
100,654 | Tissue processing and freezing after surgery | 1 | null | https://www.protocols.io/view/tissue-processing-and-freezing-after-surgery-dein3cde | Bettina Ergün | TITLE: Tissue processing and freezing after surgery
AUTHORS: Bettina Ergün
[DESCRIPTION]
The aim of this protocol is to document the processing of fresh tissue after surgery.
The collected tissue must also be documented in the files which are linked down below
The protocol descibes in detail how to process the res... | ["[Sample processing in the lab] Fresh tissue should be processed on the same day if possible, but can be stored at 4°C for a maximum of overnight.\nTo reduce pathogens, the tissue should be incubated for 60 min atRoom temperature", "[Sample processing in the lab]", "[Sample processing in the lab]", "[Sample processing... |
49,399 | Efficient third generation lentiviral particle production | 4 | dx.doi.org/10.17504/protocols.io.bugxntxn | https://www.protocols.io/view/efficient-third-generation-lentiviral-particle-pro-bugxntxn | Michelle Newbery, Simon Maksour, Amy Hulme, Neville Ng, Mirella Dottori, Lezanne Ooi | TITLE: Efficient third generation lentiviral particle production
AUTHORS: Michelle Newbery, Simon Maksour, Amy Hulme, Neville Ng, Mirella Dottori, Lezanne Ooi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The overexpression of a gene of interest by third generation lent... | ["[Lentiviral transfection]\nMaintain HEK293T cells in 0.15 mL/cm2 (e.g. 12 mL in 75 cm2 flask) animal product free FreeStyle 293 Expression Medium, foetal serum free DMEM/F12 + 2 mM GlutaMAX + 5% Knock Out Serum Replacement or DMEM/F12 + 2 mM GlutaMAX + 5% FBS. Include 50 U/mL Penicillin and Streptomycin to reduce ris... |
72,237 | Protocol 6: Selecting for Spizellomyces punctatus transformants | 4 | dx.doi.org/10.17504/protocols.io.dm6gpjjdpgzp/v1 | https://www.protocols.io/view/protocol-6-selecting-for-spizellomyces-punctatus-t-cismuec6 | Sarah M Prostak, Edgar M Medina, Erik Kalinka, Lillian Fritz-Laylin | TITLE: Protocol 6: Selecting for Spizellomyces punctatus transformants
AUTHORS: Sarah M Prostak, Edgar M Medina, Erik Kalinka, Lillian Fritz-Laylin
[DESCRIPTION]
Once the co-culture IM plates have grown for four days, it is time to select Sp transformants. Selecting these transformants requires harvesting the cells fr... | ["[Steps] For each plasmid transformed, prepare a 50 mL conical with 30 mL of DS solution.", "[Steps] From the appropriate conical for the plate to be harvested, remove 1 mL of DS.", "[Steps] Distribute the DS among the four quadrants of the corresponding IM plate.", "[Steps] Incubate at Room temperature for 5 min.", "... |
73,284 | Chloroform-methanol protein precipitation from microalgae and Pierce BCA assay | 4 | dx.doi.org/10.17504/protocols.io.yxmvm2e25g3p/v1 | https://www.protocols.io/view/chloroform-methanol-protein-precipitation-from-mic-cjtcuniw | Ying-Yu Hu, Christopher Lord, Zoe V Finkel | TITLE: Chloroform-methanol protein precipitation from microalgae and Pierce BCA assay
AUTHORS: Ying-Yu Hu, Christopher Lord, Zoe V Finkel
[DESCRIPTION]
Chlorophyll, phospholipids, sucrose, glycerol and some detergent in crude protein extracted from microalgae can interfere the Pierce BCA protein assay. In order to rem... | ["[Reagent preparation] Tris buffer 5 mM (pH 8.0)\nAdd 500 µL 1 MpH 8.0 Tris into 100 mL MilliQ water", "[Reagent preparation] 20% Sarcosine\nDilute 2 part 30% N-lauroylsarcosine sodium salt with 1 part 5 mM (pH 8.0) Tris buffer", "[Protein precipitation] Thaw protein extract", "[Protein precipitation] Turn on refr... |
23,198 | cDNA synthesis using the Applied BiosystemsTM High-Capacity cDNA Reverse Transcription Kit | null | dx.doi.org/10.17504/protocols.io.2v6ge9e | null | Lisa-Maria Rosenthal, Giang Tong, Katharina Schmitt | TITLE: cDNA synthesis using the Applied BiosystemsTM High-Capacity cDNA Reverse Transcription Kit
AUTHORS: Lisa-Maria Rosenthal, Giang Tong, Katharina Schmitt
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">cDNA synthesis using the Applied Biosystems</span><span sty... | ["Allow the kit components to thaw on ice.Prepare the 2X RT master mix on ice. AB1ComponentVolume (µL)210X RT Buffer2.0325X dNTP Mix (100 mM)0.8410X RT Random Primers2.05MultiScribeTMReverse Transcriptase1.06RNase Inhibitor1.07Nuclease-free H2O3.28Total per reaction10.0 Place the 2X RT master mix on ice and mix gentl... |
43,852 | Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation | 2 | dx.doi.org/10.17504/protocols.io.bn3kmgkw | https://www.protocols.io/view/analysis-of-the-chromosomal-localization-of-yeast-bn3kmgkw | Vasso Makrantoni, Daniel Robertson, Adele L. Marston | TITLE: Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation
AUTHORS: Vasso Makrantoni, Daniel Robertson, Adele L. Marston
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A plethora of biological processes like gene transcription, DNA replication, DNA recom... | [] |
43,121 | Multitissue DNA methylome profiling during onset of salmon maturation | 5 | dx.doi.org/10.17504/protocols.io.bncrmav6 | https://www.protocols.io/view/multitissue-dna-methylome-profiling-during-onset-o-bncrmav6 | Amin Mohamed, Bradley Evans, Antonio Reverter, James Kijas | TITLE: Multitissue DNA methylome profiling during onset of salmon maturation
AUTHORS: Amin Mohamed, Bradley Evans, Antonio Reverter, James Kijas
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Background:</div><div class = "text-block">Atlantic salmon farming promotes growth in conditions which mean... | ["[Genomic DNA isolation, WGBS library preparation and sequencing]\nTissue samples were snap frozen in liquid Nitrogen and stored at −80 °C until genomic DNA (gDNA) was extracted using DNeasy blood and tissue kit (QIAGEN). Tissues were lysed in 360 µL of lysis solution on a Precellys 24 homogenizer for 30s at 4.0 ms−1.... |
68,221 | Laser Capture Microdissection of Tissue Functional Units for microPOTS Top-Down Proteomics | 4 | dx.doi.org/10.17504/protocols.io.ewov1nrz7gr2/v1 | https://www.protocols.io/view/laser-capture-microdissection-of-tissue-functional-ceu5tey6 | James M Fulcher, Yen-Chen Liao | TITLE: Laser Capture Microdissection of Tissue Functional Units for microPOTS Top-Down Proteomics
AUTHORS: James M Fulcher, Yen-Chen Liao
[DESCRIPTION]
In this protocol, we describe a detailed procedure for high-resolution top-down proteomic analysis of human pancreatic and kidney tissue sections using a spatial prote... | ["[Tissue collection] Load DMSO as capturing media onto the microPOTS chip. Microdroplet processing in one pot for trace samples (microPOTS) chips are polyproplyene chips designed for benchtop manipulation of low sample volumes ( < 5 μL).", "[Tissue collection] Pipette 1 µL of DMSO onto each microPOT chip well", "[Tis... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.