id float64 1.55k 110k ⌀ | title stringlengths 1 256 ⌀ | template_id float64 0 6 ⌀ | doi stringlengths 39 49 ⌀ | url stringlengths 40 92 ⌀ | authors stringlengths 1 933 ⌀ | protocol_text stringlengths 34 1.08M | steps_list stringlengths 2 269k |
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92,584 | Glucosylceramide and glucosylsphingosine analysis | 4 | dx.doi.org/10.17504/protocols.io.261gedk8wv47/v2 | https://www.protocols.io/view/glucosylceramide-and-glucosylsphingosine-analysis-c6ngzdbw | Tae-Un Han, Carley Corado | TITLE: Glucosylceramide and glucosylsphingosine analysis
AUTHORS: Tae-Un Han, Carley Corado
[DESCRIPTION]
This protocol was used to analysis glucosylceramide and glucosylsphingosine levels in mouse brain and liver.
It was also described in previous publication (Mol Cell Neurosci. 2020 Jan:102:103451, doi: 10.1016)
[S... | ["[Tissue preparation] The mouse brain and liver tissues (100-300 mg) are homogenized in 2% CHAPS solution (4 mL/g wet tissue) in 2 mL Omni homogenization tubes containing 8 mm ceramic beads", "[Tissue preparation] The homogenates are processed on the Bead Ruptor 24 (Omni International, Kennesaw, GA) for two 30 second ... |
null | null | null | dx.doi.org/10.17504/protocols.io.iyacfse | null | null | TITLE: No Title
AUTHORS:
[STEPS]
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104,309 | Construction of individuals ddRADseq libraries for macro-algae (Kelp) V.3 | 0 | dx.doi.org/10.17504/protocols.io.rm7vzjo68lx1/v3 | https://www.protocols.io/view/construction-of-individuals-ddradseq-libraries-for-dh4v38w6 | Stéphane Mauger, Komlan Avia | TITLE: Construction of individuals ddRADseq libraries for macro-algae (Kelp) V.3
AUTHORS: Stéphane Mauger, Komlan Avia
[DESCRIPTION]
This protocol describes a double digested restriction-site associated DNA (ddRADseq) procedure, that is a variation on the original RAD sequencing method (Davey & Blaxter 2011), which is... | ["[Solutions and buffers preparations] Annealing buffer stock (10x)\nAnnealing buffer composed 100 mM Tris-HCl, pH8; 500 mM NaCl and 10 mM EDTA \n5 mL\n5 mL\n1 mL \n39 mL \n \nHomogenize and autoclave.\n\nStore at Room temperature", "[Solutions and buffers preparations] 5 M sodium chloride solution (NaCl)\n29.2 g \nDis... |
77,107 | EMP 18S Illumina Amplicon Protocol | 1 | dx.doi.org/10.17504/protocols.io.ewov1b6pgr24/v2 | https://www.protocols.io/view/emp-18s-illumina-amplicon-protocol-cpitvken | Linda A. Amaral-Zettler, Markus Bauer, Donna Berg-Lyons, Jason Betley, J. Greg Caporaso, Hugh W. Ducklow, Noah Fierer, Louise Fraser, Jack A. Gilbert, Niall Gormley, James Huntley, Susan M. Huse, Janet K. Jansson, Simon N. Jarman, Rob Knight, Chris L. Lauber, Elizabeth A. McCliment, Sarah M. Owens, Geoff Smith, Luke Th... | TITLE: EMP 18S Illumina Amplicon Protocol
AUTHORS: Linda A. Amaral-Zettler, Markus Bauer, Donna Berg-Lyons, Jason Betley, J. Greg Caporaso, Hugh W. Ducklow, Noah Fierer, Louise Fraser, Jack A. Gilbert, Niall Gormley, James Huntley, Susan M. Huse, Janet K. Jansson, Simon N. Jarman, Rob Knight, Chris L. Lauber, Elizabeth... | ["Amplify samples in triplicate, meaning each sample will be amplified in 3 replicate 25-µL PCR reactions.", "Pool triplicate PCR reactions for each sample into a single volume (75 µL).", "Run amplicons from each sample on an agarose gel.", "Quantify amplicons with Quant-iT PicoGreen dsDNA Assay Kit (follow manufacture... |
62,622 | Dynathri ve CBD Gummies Canada THE MOST POPULAR CBD GUMMY BEARS IN UNITED | 1 | dx.doi.org/10.17504/protocols.io.x54v9ykbpg3e/v1 | https://www.protocols.io/view/dynathri-ve-cbd-gummies-canada-the-most-popular-cb-b9d6r29e | Dynathrive CBD Gummies Canada | TITLE: Dynathri ve CBD Gummies Canada THE MOST POPULAR CBD GUMMY BEARS IN UNITED
AUTHORS: Dynathrive CBD Gummies Canada
[DESCRIPTION]
Dynathrive CBD Gummies Canada
Dynathrive CBD Gummies Canada - You plan to recuperate just as truly feel over and above anyone's expectations previously! However when you are encount... | ["[Dynathri ve CBD Gummies Canada THE MOST POPULAR CBD GUMMY BEARS IN UNITED STATES READ HERE REVIEWS, BENEFITS, SIDE EFFECT, INGREDIENTS, DOES IT REALLY WORK? IS IT SAFE? BUY NOW GET INSTANTLY]", "[Dynathri ve CBD Gummies Canada THE MOST POPULAR CBD GUMMY BEARS IN UNITED STATES READ HERE REVIEWS, BENEFITS, SIDE EFFEC... |
86,378 | Generating regionally specified astrocytes | 4 | dx.doi.org/10.17504/protocols.io.261ged3qov47/v1 | https://www.protocols.io/view/generating-regionally-specified-astrocytes-cykixuue | Tyra Fraser, Lachlan Thompson | TITLE: Generating regionally specified astrocytes
AUTHORS: Tyra Fraser, Lachlan Thompson
[DESCRIPTION]
This protocol outlines the differentiation of stem cells into regionally specific ventral midbrain and cortical astrocytes.
The protocol is a 4–5-month procedure and requires media changes 3 times a week.
[GUIDELIN... | ["[Preparation] Prepare astrocyte base media by combining;\n484.5 mL Advanced DMEM/F12\n5 mLGlutaMAX\n5 mL N2\n5 mL ANTI-ANTI\n500 µL B-27+VITA", "[Sphere formation] Disassociate D13 VMDA diff or D19 Cortex diff using Accutase for3 min to 6 min \nCollect cells as small clumps and inhibit using respective diff media + R... |
55,068 | Transplanting Arabidopsis | 1 | dx.doi.org/10.17504/protocols.io.bzz4p78w | https://www.protocols.io/view/transplanting-arabidopsis-bzz4p78w | Meghan Burns, Lynn Doran | TITLE: Transplanting Arabidopsis
AUTHORS: Meghan Burns, Lynn Doran
[DESCRIPTION]
Transplanting Arabidopsis grown from seed on agar plates to soil trays.
[BEFORE_START]
Seedlings will be ready to transplant in approximately 10 to 14 days after planting when they've formed one to two true leaves. Do not wait too... | ["Look at the Arabidopsis on the agar plate and determine which one looks the best. Typically this means it is the plant with the longest roots and greenest leaves.", "Take a pair of tweezers and place them at the base of the plant. Pull up very slowly, ensuring that all of the roots come out of the agar in one piece."... |
55,541 | Protocol for CUBIC Clearing and Whole Mount Imaging of Mouse Lung Lobes | 1 | dx.doi.org/10.17504/protocols.io.b2gvqbw6 | https://www.protocols.io/view/protocol-for-cubic-clearing-and-whole-mount-imagin-b2gvqbw6 | Justinn Barr, Jamie Verheyden, Xin Sun | TITLE: Protocol for CUBIC Clearing and Whole Mount Imaging of Mouse Lung Lobes
AUTHORS: Justinn Barr, Jamie Verheyden, Xin Sun
[DESCRIPTION]
This protocol is for Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis (CUBIC) of mouse lung tissue for whole lobe imaging using Zeiss Lightshee... | ["[Clearing preparations] Euthanize mice by CO2 inhalation.", "[Clearing preparations] To clear blood from the lungs perfuse transcardially the animals with 1xPBS. Gravity inflate lungs with 4% paraformaldehyde (PFA) and fix overnight at 4 °C .", "[Clearing preparations] Wash the lungs at least 3x for 10 minutes with 1... |
91,850 | UroMOCA and ColoMOCA implantation protocol | 4 | dx.doi.org/10.17504/protocols.io.rm7vzxq78gx1/v1 | https://www.protocols.io/view/uromoca-and-colomoca-implantation-protocol-c5xiy7ke | Brett Hanzlicek, Margot Damsaser, Dennis Bourbeau | TITLE: UroMOCA and ColoMOCA implantation protocol
AUTHORS: Brett Hanzlicek, Margot Damsaser, Dennis Bourbeau
[DESCRIPTION]
This protocol describes the implantation of our bladder (UroMOCA) and bowel device (ColoMOCA) in pigs.
[STEPS]
SECTION: Anesthesia
1. Anesthetize/prep the animal for surgery.
Aseptic ... | ["[Anesthesia] Anesthetize/prep the animal for surgery.\n\n Aseptic procedures by trained personnel are utilized during the surgery. All tools are sterilized prior to insertion. Drapes will be used and personnel performing surgery will wear clean surgical scrubs, sterile gowns/gloves and... |
47,452 | DNA extraction and quantification | 1 | dx.doi.org/10.17504/protocols.io.bsj4ncqw | https://www.protocols.io/view/dna-extraction-and-quantification-bsj4ncqw | Rene Flores Clavo, Cristian Daniel Asmat Ortega, Nataly Ruiz Quinones | TITLE: DNA extraction and quantification
AUTHORS: Rene Flores Clavo, Cristian Daniel Asmat Ortega, Nataly Ruiz Quinones
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">DNA extraction and quantification</span></div></div>
[STEPS]
?. Resuspend previously grown bact... | ["Resuspend previously grown bacterial cells (approximately 50 mg of bacterial fresh biomass) in 400 µL de TE 1X (10mM of Tris-HCl, 1mM of EDTA pH 8,0).", "Add 1 µL of lysozyme (100mg/ml) shortly vórtex, and incubate for 1 h at 37 °C.", "Add 67,5 µL of SDS 10 % (pre-heated at 65 °C) and 2,5 µL of Proteinase K (10 mg/mL... |
98,364 | Traditional Fishing Knowledge and Fishing Policy Analysis | 0 | dx.doi.org/10.17504/protocols.io.5qpvok547l4o/v1 | https://www.protocols.io/view/traditional-fishing-knowledge-and-fishing-policy-a-dca42sgw | Kai Bloom | TITLE: Traditional Fishing Knowledge and Fishing Policy Analysis
AUTHORS: Kai Bloom
[DESCRIPTION]
Examine how traditional fishing knowledge is not being passed down to the new generations of fishermen in Mo'orea, French Polynesia, thus causing fishing policy to be simplified. Additionally, examine the effects that sim... | ["[Examining The Loss of Traditional Fishing Knowledge] Identify groups of local fishermen, both with traditional knowledge and new-generation fishermen", "[Examining The Loss of Traditional Fishing Knowledge] Conduct interviews with fishermen", "[Examining The Loss of Traditional Fishing Knowledge] Transcribe and docu... |
43,598 | Staining of single-cell suspensions for scMEP mass cytometry analysis | 4 | dx.doi.org/10.17504/protocols.io.bntnmeme | https://www.protocols.io/view/staining-of-single-cell-suspensions-for-scmep-mass-bntnmeme | Felix Hartmann | TITLE: Staining of single-cell suspensions for scMEP mass cytometry analysis
AUTHORS: Felix Hartmann
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Staining of single-cell suspensions for scMEP analysis by mass cytometry (CyTOF). This protocol includes 1) surface staining for 30min at RT, 2) PFA-fi... | ["[Obtain single cell suspension]\nPrepare single cell suspensions in FACS tubes as described in the following protocol:\n{\"blocks\":[{\"key\":\"d7ogq\",\"text\":\"Preparation of single cell suspensions to be analyzed for metabolic and phenotypic features by scMEP mass cytometry analysis. This protocols spans 1) optio... |
32,435 | Materials and Methods - Transcriptomic profiling identifies novel transcripts, isomorphs and lncRNAs in Paracoccidioides brasiliensis | null | dx.doi.org/10.17504/protocols.io.bbwtipen | null | Fabiano Menegidio, David Aciole Barbosa, Regina Costa de Oliveira, Daniela L. Jabes, Luiz R. Nunes | TITLE: Materials and Methods - Transcriptomic profiling identifies novel transcripts, isomorphs and lncRNAs in Paracoccidioides brasiliensis
AUTHORS: Fabiano Menegidio, David Aciole Barbosa, Regina Costa de Oliveira, Daniela L. Jabes, Luiz R. Nunes
[STEPS]
?. | [] |
92,763 | Baermann Funnel Extraction | 1 | null | https://www.protocols.io/view/baermann-funnel-extraction-c6t3zeqn | Casey A Schlenker | TITLE: Baermann Funnel Extraction
AUTHORS: Casey A Schlenker
[DESCRIPTION]
Baermann Funnel Extraction
[STEPS]
SECTION: Sample Preparation
1. Sieve soil to 2mm
SECTION: Sample Preparation
2. Weigh out 50 g in a weigh boat
SECTION: Sample Preparation
3. Place tissue paper on top of Baermann funnel apparatus
SECTION: S... | ["[Sample Preparation] Sieve soil to 2mm", "[Sample Preparation] Weigh out 50 g in a weigh boat", "[Sample Preparation] Place tissue paper on top of Baermann funnel apparatus", "[Sample Preparation] Place weighed and sieved soil on top of filter paper", "[Sample Preparation] Fill Baermann funnel with DI water using a D... |
25,033 | Processing Single Cell Calling Card Sequencing Data | null | dx.doi.org/10.17504/protocols.io.4phgvj6 | null | Arnav Moudgil, Rob Mitra | TITLE: Processing Single Cell Calling Card Sequencing Data
AUTHORS: Arnav Moudgil, Rob Mitra
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Here we present a computational pipeline for processing single cell calling card (scCC) data. These data will have been generated from single cell RNA-se... | ["[Preamble]\nThe objective of this protocol is to take sequencing reads from single cell calling cards (scCC) library and process them into a CCF (calling card format; .ccf) file. A CCF file is a modified BED file (BED3+3) that concisely enumerates every transposition event in the sequenced library.CCF files typically... |
75,472 | Protocol for investigating neuroprotective effect of the methanol leaf extract of Mallotus oppositifolius in lipopolysaccharide neuro-inflammation-associated depression in mice. | 1 | dx.doi.org/10.17504/protocols.io.bp2l693kzlqe/v1 | https://www.protocols.io/view/protocol-for-investigating-neuroprotective-effect-cmxqu7mw | KENNEDY KWAMI EDEM KUKUIA, Patrick Amoateng, Ferka Yaw Takyi, Kelvin Kofi Adutwum-Ofosu, Frimpong Appiah | TITLE: Protocol for investigating neuroprotective effect of the methanol leaf extract of Mallotus oppositifolius in lipopolysaccharide neuro-inflammation-associated depression in mice.
AUTHORS: KENNEDY KWAMI EDEM KUKUIA, Patrick Amoateng, Ferka Yaw Takyi, Kelvin Kofi Adutwum-Ofosu, Frimpong Appiah
[DESCRIPTION]
Despit... | ["[COLLECTION OF MALLOTUS OPPOSITIFOLIUS LEAVES] Mallotus oppositifolius plant is common shrub. However, for this work, we collected the plant material from Centre for Plant Medicine Research, Akuapem-Mampong in the Eastern Region of Ghana. The geographical coordinates have been provided in the attached document.\nSome... |
103,987 | Techniques for the detection and counting of starch particles in the atmosphere | 0 | dx.doi.org/10.17504/protocols.io.n2bvjnzzwgk5/v1 | https://www.protocols.io/view/techniques-for-the-detection-and-counting-of-starc-dhst36en | Claudio F. Pérez, Soledad Maciel Ramos Mejía, María Gassmann, Rubén F. Iacono, Mauricio C. De Marzi, Mauro Covi | TITLE: Techniques for the detection and counting of starch particles in the atmosphere
AUTHORS: Claudio F. Pérez, Soledad Maciel Ramos Mejía, María Gassmann, Rubén F. Iacono, Mauricio C. De Marzi, Mauro Covi
[DESCRIPTION]
Suspended particles in the atmosphere (aerosols) can impact visibility, the radiation budget, arc... | ["[Sampling design] Decide what type of sampling to deploy in the field (fig.1).", "[Preparation of adhesive surfaces] Prepare sticky slides spreading the adhesive mixture with a soft brush. The duration of exposure varies according to the research goals.", "[Preparation of the adhesive mixture and mounting media] Seve... |
66,121 | What is TruKeto? | 1 | dx.doi.org/10.17504/protocols.io.ewov1nbwygr2/v1 | https://www.protocols.io/view/what-is-truketo-ccthswj6 | orgtruketo | TITLE: What is TruKeto?
AUTHORS: orgtruketo
[DESCRIPTION]
TruKetoDietary improvements can help a person with getting more fit quickly and train their body to rely upon fat stores for heat creation. Without a doubt, even yet, somewhat level of weight decrease regimens work. Before purchasing any fat-consuming thing, ... | [] |
44,737 | Modified Moore Swab Method for Microbiological Surface Waters Sampling | 1 | dx.doi.org/10.17504/protocols.io.bpw9mph6 | https://www.protocols.io/view/modified-moore-swab-method-for-microbiological-sur-bpw9mph6 | Enrique Delgado | TITLE: Modified Moore Swab Method for Microbiological Surface Waters Sampling
AUTHORS: Enrique Delgado
[DESCRIPTION]
This protocol describes the use of modified Moore swabs to collect surface water samples for microbiological analysis.
[STEPS]
SECTION: Modified Moore Swab (MMS) Preparation
1. Filtration cloth. Cut ... | ["[Modified Moore Swab (MMS) Preparation] Filtration cloth. Cut a 92 x 95 cm2 cheesecloth grade #90 piece and fold it horizontally. Fold it again to its half size and stretch. Fold again each of the sides until they touch each other and press it to get the cloth as flat as possible. Then, tightly roll the cheesecloth t... |
98,644 | SARS-CoV-2 incursion scenario in the city Fantastica v2 | 1 | dx.doi.org/10.17504/protocols.io.kqdg326xpv25/v1 | https://www.protocols.io/view/sars-cov-2-incursion-scenario-in-the-city-fantasti-dcju2unw | Benjamin Schwessinger | TITLE: SARS-CoV-2 incursion scenario in the city Fantastica v2
AUTHORS: Benjamin Schwessinger
[DESCRIPTION]
This protocol is part of the ANU Biosecurity mini-research project #2 "An SARS-CoV-2 incursion scenario: Genomics, phylogenetics, and incursions." This mini-research project aims to highlight the power of combin... | ["Now you import all the data needed in Geneious. Track and drop the \"FantasticaSARSCoV2Sequences\" and the reference sequence \"MN908947.3\" into this in class folder.\n\n \nYou are ready for all your analysis now.", "[Section II: Generation of a multiple sequence alignment in Geneious] Now you will select all sequen... |
33,091 | Association between the rs1544410 polymorphism in the vitamin D receptor (VDR) gene and insulin secretion after gestational diabetes mellitus | null | dx.doi.org/10.17504/protocols.io.bcjbiuin | https://www.protocols.io/view/association-between-the-rs1544410-polymorphism-in-bcjbiuin | Nael Shaat, Anastasia Katsarou, Bushra Shahida, Rashmi B Prasad, Karl Kristensen, Tereza Planck | TITLE: Association between the rs1544410 polymorphism in the vitamin D receptor (VDR) gene and insulin secretion after gestational diabetes mellitus
AUTHORS: Nael Shaat, Anastasia Katsarou, Bushra Shahida, Rashmi B Prasad, Karl Kristensen, Tereza Planck
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block... | [] |
92,799 | SCN Egg Extraction Protocol | 4 | dx.doi.org/10.17504/protocols.io.14egn318yl5d/v1 | https://www.protocols.io/view/scn-egg-extraction-protocol-c6u7zezn | Casey A Schlenker | TITLE: SCN Egg Extraction Protocol
AUTHORS: Casey A Schlenker
[DESCRIPTION]
OSU full SCN egg extraction protocol
[STEPS]
SECTION: Core Processing
1. Obtain soil cores, a small plastic bin, and anaccompanying mesh screen. Grab one Ziploc bag/container of a sample and pour it out onto the screen, on top of the bin. Car... | ["[Core Processing] Obtain soil cores, a small plastic bin, and anaccompanying mesh screen. Grab one Ziploc bag/container of a sample and pour it out onto the screen, on top of the bin. Carefully break up large pieces with your fingers by rolling cores against the mesh. Do not crush pieces; break up cores gently to avo... |
60,802 | SJB Artificial Seawater Medium Protocol | 4 | dx.doi.org/10.17504/protocols.io.rm7vzy615lx1/v1 | https://www.protocols.click/view/sjb-artificial-seawater-medium-protocol-b7mark2e | Shelby J Barnes, Michael Henson, Cameron Thrash | TITLE: SJB Artificial Seawater Medium Protocol
AUTHORS: Shelby J Barnes, Michael Henson, Cameron Thrash
[DESCRIPTION]
SJB Artificial Seawater Medium Protocol
SJB Artificial Seawater Medium is an amended version of JW and MWH generations of Thrash Lab artificial seawater medium.
[STEPS]
SECTION: Stock Preparation
... | ["[Stock Preparation] Prepare the following stocks prior to medium assembly.", "[Base Salts] Using an acid-washed and autoclaved one Liter screw-top Pyrex bottle, combine basic salts in 997.4095 mL MilliQ-filtered water. (stock volumes subtracted)", "[Stock Addition] Stocks should be added in a Biosafety cabinet or hoo... |
null | null | null | dx.doi.org/10.17504/protocols.io.nycdfsw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa ... | [] |
85,694 | Analysis of immofluorescence images in ImageJ | 4 | dx.doi.org/10.17504/protocols.io.x54v9py4zg3e/v1 | https://www.protocols.io/view/analysis-of-immofluorescence-images-in-imagej-cxw6xphe | rachel.bates | TITLE: Analysis of immofluorescence images in ImageJ
AUTHORS: rachel.bates
[DESCRIPTION]
Analysis at a single cell level of images taken on confocal
[STEPS]
1. Import image into ImageJ as a TIFF file.
2. Set scale for Image by going to Analyze --> set scale. Set distance in pixels and unit of length to appropriate v... | ["Import image into ImageJ as a TIFF file.", "Set scale for Image by going to Analyze --> set scale. Set distance in pixels and unit of length to appropriate values for objective images were collected with.", "Convert image to 8 bit going to Image --> type --> 8 bit.", "Open up ROI manager using Analyser --> tools --> ... |
42,683 | Lab 1 Notebook | 3 | null | https://www.protocols.io/view/lab-1-notebook-bmw3k7gn | TITLE: Lab 1 Notebook
AUTHORS:
[STEPS] | [] | |
60,196 | Purification of lysosomes using dextran-conjugated SPIONs | 4 | dx.doi.org/10.17504/protocols.io.bp2l61dr1vqe/v1 | https://www.protocols.io/view/purification-of-lysosomes-using-dextran-conjugated-b62crgaw | William Hancock-Cerutti, Arun Kumar Tharkeshwar, Shawn M Ferguson, Pietro De Camilli | TITLE: Purification of lysosomes using dextran-conjugated SPIONs
AUTHORS: William Hancock-Cerutti, Arun Kumar Tharkeshwar, Shawn M Ferguson, Pietro De Camilli
[DESCRIPTION]
This method describes the usage of dextran-conjugated superparamagnetic iron nanoparticles (SPIONs) to purify a lysosome fraction from culture... | [] |
67,401 | Testo Chews Reviews - Is It 100% Safe & Effective For Building Muscle! Price | 3 | dx.doi.org/10.17504/protocols.io.j8nlkkxbdl5r/v1 | https://www.protocols.io/view/testo-chews-reviews-is-it-100-safe-amp-effective-f-cd3hs8j6 | Testo Chews | TITLE: Testo Chews Reviews - Is It 100% Safe & Effective For Building Muscle! Price
AUTHORS: Testo Chews
[DESCRIPTION]
Testo Chews Reviews
[STEPS] | [] |
40,862 | Copy of Enzyme-linked immunosorbent assay (ELISA) for studying the presence of anti-Salmonella antibody in layer hen's egg yolks. | 6 | dx.doi.org/10.17504/protocols.io.bj56kq9e | https://www.protocols.io/view/copy-of-enzyme-linked-immunosorbent-assay-elisa-fo-bj56kq9e | Angel Justiz-Vaillant | TITLE: Copy of Enzyme-linked immunosorbent assay (ELISA) for studying the presence of anti-Salmonella antibody in layer hen's egg yolks.
AUTHORS: Angel Justiz-Vaillant
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Enzyme-linked immunosorbent assay (ELISA) for ... | ["U-shaped bottom's ninety-six well polystyrene microplate purchased at Sigma-Aldrich, St. Louis USA was incubated with (2 µg/well) of the LPS (Sigma –Aldrich) from Salmonella Typhimurium in coating buffer (overnight at 4 ºC.)", "The microtiter plates was washed four times, with 10 % PBS-Tween-20.", "The microplate was... |
60,516 | Next Generation Sequencing of HIV-1 Drug Resistant Mutations | 4 | dx.doi.org/10.17504/protocols.io.bp2l618k5vqe/v2 | https://www.protocols.io/view/next-generation-sequencing-of-hiv-1-drug-resistant-b7ccrisw | Brenna M McGruder Rawson | TITLE: Next Generation Sequencing of HIV-1 Drug Resistant Mutations
AUTHORS: Brenna M McGruder Rawson
[DESCRIPTION]
The Florida Department of Health's Bureau of Public Health Laboratories in Jacksonville has developed a protocol for the Next Generation Sequencing (NGS) of HIV, primarily for the purpose of drug-resista... | ["[RNA Extraction] RNA Extraction has been verified using the following methods", "[RNA Extraction] Qiagen QIAmp Viral RNA Mini Kit (DSP or RUO)\nhttps://www.qiagen.com/us/products/diagnostics-and-clinical-research/sample-processing/qiaamp-viral-rna-kits/", "[RNA Extraction] Thermofisher MagMAX Viral/Pathogen II (MVP I... |
66,344 | What Is Iron Warrior Testo Thrust Review, Side Effects, Benefits, And Price? | 3 | dx.doi.org/10.17504/protocols.io.kqdg3popql25/v1 | https://www.protocols.io/view/what-is-iron-warrior-testo-thrust-review-side-effe-cc2gsybw | Iron Warrior Testo Thrust | TITLE: What Is Iron Warrior Testo Thrust Review, Side Effects, Benefits, And Price?
AUTHORS: Iron Warrior Testo Thrust
[DESCRIPTION]
https://www.outlookindia.com/outlook-spotlight/iron-warrior-testo-thrust-reviews-canada-scam-alert-muscle-flow-pills-website-news-204145
[STEPS] | [] |
9,583 | 4AA Mix | 1 | dx.doi.org/10.17504/protocols.io.mkpc4vn | https://www.protocols.io/view/4aa-mix-mkpc4vn | Erçağ Pinçe | TITLE: 4AA Mix
AUTHORS: Erçağ Pinçe
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">4 amino acid mixture for MMH1 </div></div>
[STEPS]
?. [Mix the ingredients ]
40 mL ddH2O(milliQ)620 mg L-Histidine [100mM]596 mg L-Methionine [100mM] 524 mg L-Leucine [100mM]476 mg L-Threonine [100mM]Stir for overni... | ["[Mix the ingredients ]\n40 mL ddH2O(milliQ)620 mg L-Histidine [100mM]596 mg L-Methionine [100mM] 524 mg L-Leucine [100mM]476 mg L-Threonine [100mM]Stir for overnight at 37.5C. Adjust pH to 7.0.Filter sterilize and store in the cold room(+4°C)"] |
87,697 | Synthetic Procedure of Dimethyl 2-methoxy-4,4'-biphenyldicarboxylate | 6 | dx.doi.org/10.17504/protocols.io.j8nlko9o5v5r/v1 | https://www.protocols.io/view/synthetic-procedure-of-dimethyl-2-methoxy-4-4-39-b-czvrx656 | Lisa.Stanley, Caroline Hoyt, Gregg T. Beckham | TITLE: Synthetic Procedure of Dimethyl 2-methoxy-4,4'-biphenyldicarboxylate
AUTHORS: Lisa.Stanley, Caroline Hoyt, Gregg T. Beckham
[DESCRIPTION]
Lignin represents the largest source of biomass-derived aromatic chemicals and is an ideal supplement or alternative to petroleum-based feedstocks. In connection with eff... | ["[Synthetic Procedure]", "[Synthetic Procedure] Synthesis of Methyl 4-(tosyloxy)benzoate and Methyl 3-methoxy-4-(tosyloxy)benzoate. Methyl paraben (6.6194 g, 0.0435 mol), dimethylaminopyridine (0.5315 g, 0.00435 mol), and triethylamine (8.805 g, 0.0870 mol) were charged into a round-bottom flask. 60 mL of anhydrous di... |
28,243 | MojoSort™ Human Pan Monocyte Isolation Kit Column Protocol | null | dx.doi.org/10.17504/protocols.io.7tthnnn | null | Sam Li | TITLE: MojoSort™ Human Pan Monocyte Isolation Kit Column Protocol
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. T... | ["[Platelet Removal Protocol]\nDilute blood with 2-4 times (volume/volume) 1X PBS.", "[Platelet Removal Protocol]\nCarefully layer diluted blood over 12.5 mL of isolation medium in a 50mL tube.", "[Platelet Removal Protocol]\nCentrifuge at 400xg for 25 minutes at room temperature in a swinging-bucket rotor without the ... |
97,507 | CODA (part 1): setting up environment and preparing sample dataset | HuBMAP | JHU-TMC | 0 | dx.doi.org/10.17504/protocols.io.q26g71rpkgwz/v1 | https://www.protocols.io/view/coda-part-1-setting-up-environment-and-preparing-s-dbgb2jsn | Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Sashank Reddy, Ashley Kiemen, Denis Wirtz | TITLE: CODA (part 1): setting up environment and preparing sample dataset | HuBMAP | JHU-TMC
AUTHORS: Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Sashank Reddy, Ashley Kiemen, Denis Wirtz
[DESCRIPTION]
CODA workflow part 1. setting up environment and preparing dataset
[STEPS]
SECTION: Software requirements
1. MATLAB MA... | ["[Software requirements] MATLAB MATLAB (mathworks.com)\nImage processing toolbox Image Processing Toolbox - MATLAB (mathworks.com)\nDeep learning toolbox Deep Learning Toolbox - MATLAB (mathworks.com)\nMATLAB - Resnet50 model MATLAB resnet50 (mathworks.com)", "[Software requirements] Aperio ImageScope Aperio ImageScop... |
97,099 | USDA LTAR Common Experiment measurement: Concentration of phosphorus, potassium, and sulfur in aboveground biomass | 1 | dx.doi.org/10.17504/protocols.io.14egn6bnql5d/v1 | https://www.protocols.io/view/usda-ltar-common-experiment-measurement-concentrat-da3j2gkn | John L. Kovar, Ann Marie Fortuna | TITLE: USDA LTAR Common Experiment measurement: Concentration of phosphorus, potassium, and sulfur in aboveground biomass
AUTHORS: John L. Kovar, Ann Marie Fortuna
[DESCRIPTION]
An adequate supply of plant nutrients is an important part of sustainable crop production and the overall health of a crop. Knowing the amoun... | ["[Sample collection] In most cases, biomass samples will be collected at each harvest, potentially resulting in multiple samples per year.", "[Sample collection] For the common experiment, it is important to collect samples from BAU and ASP treatments when plants are at the same phenological (developmental) stage.", "... |
101,288 | Fresh 80% EtOH | 0 | dx.doi.org/10.17504/protocols.io.5qpvokkz9l4o/v1 | https://www.protocols.io/view/fresh-80-etoh-de6g3hbw | Menglin WANG | TITLE: Fresh 80% EtOH
AUTHORS: Menglin WANG
[DESCRIPTION]
To make molecular level fresh 80% ethanol.
[STEPS]
SECTION: 80% EtOH
1. Use a 50ml Falcon tube to mix 10 mL and 40 mL .
| ["[80% EtOH] Use a 50ml Falcon tube to mix 10 mL and 40 mL ."] |
null | null | null | dx.doi.org/10.17504/protocols.io.dsm6c5 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
This protocol is a minor modification of that reported by Culley and Steward (2007). As the starting point for this protocol, we assume that viruses have been collected on an aluminum oxide 0.02-µm syringe-tip filter (Anotop, Whatman), but other filters capable of capturing viru... | [] |
50,082 | Evaluation of elasticity of the pancreas by Acoustic Radiation Force Impulse (ARFI) in acute pancreatitis patients: A systematic review and meta-analysis (protocol) | 1 | dx.doi.org/10.17504/protocols.io.bu6anzae | https://www.protocols.io/view/evaluation-of-elasticity-of-the-pancreas-by-acoust-bu6anzae | Kazuma Rifu, Jun Watanabe, Hideki Sasanuma, Nobuyuki Taniguchi | TITLE: Evaluation of elasticity of the pancreas by Acoustic Radiation Force Impulse (ARFI) in acute pancreatitis patients: A systematic review and meta-analysis (protocol)
AUTHORS: Kazuma Rifu, Jun Watanabe, Hideki Sasanuma, Nobuyuki Taniguchi
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div cla... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.nx6dfre | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?.
?. | [] |
100,934 | Metabarcoding PCR Protocol | 0 | dx.doi.org/10.17504/protocols.io.6qpvr8842lmk/v1 | https://www.protocols.io/view/metabarcoding-pcr-protocol-dete3eje | Mirayana Marcelino Barros, Tiago Pereira, Alejandro De Santiago Perez, Hunter Powell, Jenna Brown | TITLE: Metabarcoding PCR Protocol
AUTHORS: Mirayana Marcelino Barros, Tiago Pereira, Alejandro De Santiago Perez, Hunter Powell, Jenna Brown
[DESCRIPTION]
18S Metabarcoding PCR Protocol using Invitrogen Platinum Hot Start PCR 2X Master Mix (REF 13000014) buffer.
[STEPS]
SECTION: Preparing the PCR tubes
1. Label PCR t... | ["[Preparing the PCR tubes] Label PCR tubes and place them under a UV light for 10 min.", "[Metabarcoding PCR] Transfer 18 µL of the master mix to each PCR tube.", "[Metabarcoding PCR] Transfer 5 µL of the DNA template to each PCR tube.", "[Metabarcoding PCR] Transfer 2 µL of the Metabarcoding Primer to the assigned PC... |
null | null | null | dx.doi.org/10.17504/protocols.io.rtrd6m6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Bloodstream infections caused by carbapenemase-producing Enterobacteriaceae (CPE) are associated with treatment failure and increased mortality. Detection of CPE from blood cultures (BC) by standard methods takes 16-72 hours, which can delay the initiation of appropriate anti... | ["Draw 1-3 ml of blood culture fluid from blood culture bottles that have been flagged positive by the blood culture automate (e.g. BD Bactex FX) and that contain Gram-negative bacilli on Gram-stain.\nAdd 1 ml of blood culture fluid in a 1.5 ml reaction tube (e.g. Eppendorf).", "[Hemolysis of red blood cells] {\"blocks... |
105,730 | A systematic review protocol for Biometrics in healthcare: strategies for improving safety and privacy of patients’ records in sub-Saharan Countries | 1 | dx.doi.org/10.17504/protocols.io.261ge589wg47/v1 | https://www.protocols.io/view/a-systematic-review-protocol-for-biometrics-in-hea-djha4j2e | Arnold M Hamapa, Joseph Mumba Zulu, Oswell Khondowe, Choolwe Jacobs, Mercy Wamunyima Monde, Adam Silumbwe, Lydia Hangulu | TITLE: A systematic review protocol for Biometrics in healthcare: strategies for improving safety and privacy of patients’ records in sub-Saharan Countries
AUTHORS: Arnold M Hamapa, Joseph Mumba Zulu, Oswell Khondowe, Choolwe Jacobs, Mercy Wamunyima Monde, Adam Silumbwe, Lydia Hangulu
[DESCRIPTION]
Background: The he... | ["[Methodology] The reviewers will conduct a systematic review of studies that report on strategies for implementing biometric technologies to improve the safety and privacy of patients’ records in sub-Saharan African healthcare settings and what is the impact of these strategies on healthcare outcomes within the conte... |
83,438 | Twenty-Three Body Measurements for the Somatomap App | 1 | dx.doi.org/10.17504/protocols.io.e6nvwdpr2lmk/v1 | https://www.protocols.io/view/twenty-three-body-measurements-for-the-somatomap-a-cvqnw5ve | Bea Calahong, Joel P Diaz-Fong, Sameena Karsan, Jamie Feusner | TITLE: Twenty-Three Body Measurements for the Somatomap App
AUTHORS: Bea Calahong, Joel P Diaz-Fong, Sameena Karsan, Jamie Feusner
[DESCRIPTION]
The present protocol describes the standardized measurements for the 23 body parts corresponding to the Somatomap 3D application. Somatomap 3D is a digital avatar tool which ... | ["[Standard Protocol Measurement] Neck Height:\nAsk the participant to tilt their head backward and look up. Measure from the suprasternal notch to the angle of the mandible.", "[Standard Protocol Measurement] Neck Circumference:\nAsk the participant to look straight ahead of them. Measure the circumference at the 1/2-... |
52,440 | Gradient PCR | 4 | dx.doi.org/10.17504/protocols.io.bxfypjpw | https://www.protocols.io/view/gradient-pcr-bxfypjpw | Ashwinuday | TITLE: Gradient PCR
AUTHORS: Ashwinuday
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol can be used to confirm genes or DNA of interest from a template using PCR. Also to amplify required amount of genes to larger amounts.</div></div>
[STEPS]
?. Prepare the working stock solution of... | ["Prepare the working stock solution of all the reagents required. If already prepared and stored, takeout from the refrigerator and thaw on ice.", "Prepare the following mixes each of total and 5 test samples were made for the 5 different annealing temperatures to be vaired: - Itemsnegative controltest sample (*5)ul... |
45,220 | Dental Calculus Field-Sampling Protocol (Sabin version) | 1 | dx.doi.org/10.17504/protocols.io.bqecmtaw | https://www.protocols.io/view/dental-calculus-field-sampling-protocol-sabin-vers-bqecmtaw | Susanna Sabin, James Fellows Yates | TITLE: Dental Calculus Field-Sampling Protocol (Sabin version)
AUTHORS: Susanna Sabin, James Fellows Yates
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes how to sample dental calculus from skeletal remains for biomolecular analysis. This protocol is particularly recommended ... | ["[Workstation Preparation]\nPut on two pairs of gloves.\nReplacement The outer layer will be replaced after each sampling.", "[Workstation Preparation]\nCover work surface with two layers of aluminum foil.\nReplacement The top layer of foil must be replaced after coming into contact with sampling debris. Alternatively... |
14,292 | Water preservation for flow cytometry | 1 | dx.doi.org/10.17504/protocols.io.kqdg34eo7l25/v1 | https://www.protocols.click/view/water-preservation-for-flow-cytometry-r7ud9nw | Melissa B Duhaime, Rachel Cable | TITLE: Water preservation for flow cytometry
AUTHORS: Melissa B Duhaime, Rachel Cable
[DESCRIPTION]
This method of preservation for flow cytometry was adapted from the TARA Oceans protocol for bacterial and viral flow cytometry.
This version of the protocol was edited by Rachel Cable for use in EXPORTS 2018 cruises a... | ["[Glutaraldehyde preparation] For 1.5 mL sample volume:\nAliquot 7.5 µL of 25% aqueous gluteraldehyde into each cryovial. Store aliquoted glutaraldehyde at 4°C until use.", "[Sample preservation] Using a sterile 10-ml syringe (see bleaching protocol in Step 2 if syringe is being reused) and the prepared filter cartrid... |
22,696 | Estimating the cost of HIV/AIDS and TB services at health facilities in Cambodia | null | dx.doi.org/10.17504/protocols.io.2eggbbw | null | kouland thin, Benjamin Johns | TITLE: Estimating the cost of HIV/AIDS and TB services at health facilities in Cambodia
AUTHORS: kouland thin, Benjamin Johns
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In Cambodia, HIV/AIDS and TB have historically been financed by donors, the Ministry of Health, and</div><div class = "text-bl... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.eq8bdzw | null | null | TITLE: No Title
AUTHORS:
[GUIDELINES]
SOB medium<br />
<ul>
<li>1.0% Bacto-tryptone</li>
<li>0.5% Bacto-yeast extract</li>
<li>10.0 mM NaCl</li>
<li>2.5 mM KCl</li>
<li>10.0 mM MgCl<sub>2</sub></li>
<li>10.0 mM MgSO<sub>4</sub></li>
</ul>
[STEPS]
?.
?. | [] |
93,625 | α-Synuclein Protein Preparation (Large scale) with Sedimentation and Thioflavin T Assays | 2 | dx.doi.org/10.17504/protocols.io.j8nlkop65v5r/v1 | https://www.protocols.io/view/synuclein-protein-preparation-large-scale-with-se-c7nzzmf6 | Michael X. Henderon, Lindsay Meyerdirk | TITLE: α-Synuclein Protein Preparation (Large scale) with Sedimentation and Thioflavin T Assays
AUTHORS: Michael X. Henderon, Lindsay Meyerdirk
[DESCRIPTION]
This is a collection of protocols consisting α-synuclein protein preparation (large scale), thioflavin T Assay, and sedimentation assay.
[STEPS] | [] |
37,721 | Effect of a 90 g/day low-carbohydrate diet on glycemic control, small dense low density lipoprotein, and carotid intima-media thickness in type 2 diabetic patients: an 18month randomized controlled trial protocol | 1 | dx.doi.org/10.17504/protocols.io.bg3zjyp6 | https://www.protocols.io/view/effect-of-a-90-g-day-low-carbohydrate-diet-on-glyc-bg3zjyp6 | Chin-Ying Chen, Wei Sheng Huang, Hui-Chuen Chen, Chin-Hao Chang, Long-Teng Lee, Heng-Shuen Chen, Yow-Der Kang, Wei-Chu Chie, Chyi-Feng Jan, Wei-Dean Wang, Jaw-Shiun Tsai | TITLE: Effect of a 90 g/day low-carbohydrate diet on glycemic control, small dense low density lipoprotein, and carotid intima-media thickness in type 2 diabetic patients: an 18month randomized controlled trial protocol
AUTHORS: Chin-Ying Chen, Wei Sheng Huang, Hui-Chuen Chen, Chin-Hao Chang, Long-Teng Lee, Heng-Shuen ... | ["Inclusion criteria Adults aged 20–80 years with type 2 DM 1. Diabetes for more than one year and had a poorly controlled HbA1c ≥ 58 mmol/mol (7.5%) in the previous three months, regardless of whether they received medications.", "Exclusion criteria 1. Pregnant or lactating women2. Impaired renal function with a seru... |
null | null | null | dx.doi.org/10.17504/protocols.io.ikqccvw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p> A method for concentrating aquatic microbes for single-cell analysis by Raman microspectroscopy. Modified from Hewes and Holm-Hansen (1983): A Method for Recovering Nanoplankton from Filters for Identification with the Microscope: The Filter-Transfer-Freeze (FTF) Techniqu... | [] |
30,706 | Anti-Neu5Gc Antibody Kit Protocol - ELISA | null | dx.doi.org/10.17504/protocols.io.98sh9we | null | Sam Li | TITLE: Anti-Neu5Gc Antibody Kit Protocol - ELISA
AUTHORS: Sam Li
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is essentially the same as a standard ELISA protocol, with one major exception - all animal compounds must be avoided in buffers, etc. because they contain Neu5Gc, which wil... | ["Coat the 96-well ELISA plate with the target molecule in 50mM sodium carbonate-bicarbonate buffer, pH 9.5 (up to 1µg/well in 100µl carbonate buffer, in triplicate) for at least 2 hours at ambient temperature or overnight at 4°C.", "Wash the wells 3 to 5 times with PBS.", "Block wells with PBS-T (200µl/well) for 1 hou... |
45,914 | Effect of Multiple Biofortified Food Crops-based Meals on Micronutrient Status and Immune Function in The First 1000 Days of Life in India: Study Protocol for a Randomized Controlled Feeding Trial v2.0 | 3 | dx.doi.org/10.17504/protocols.io.bq32myqe | https://www.protocols.io/view/effect-of-multiple-biofortified-food-crops-based-m-bq32myqe | Saurabh Mehta, Bryan Gannon, Camille E. Jones, Laura S. Hackl, Shoba A. Udipi, Varsha Thakker, Aparna Thorat, Wesley Bonam, Jere D. Haas, Julia Finkelstein | TITLE: Effect of Multiple Biofortified Food Crops-based Meals on Micronutrient Status and Immune Function in The First 1000 Days of Life in India: Study Protocol for a Randomized Controlled Feeding Trial v2.0
AUTHORS: Saurabh Mehta, Bryan Gannon, Camille E. Jones, Laura S. Hackl, Shoba A. Udipi, Varsha Thakker, Aparna ... | [] |
55,580 | Wastewater QC workflow in GalaxyTrakr (SSQuAWK) | 1 | null | https://www.protocols.io/view/wastewater-qc-workflow-in-galaxytrakr-ssquawk-b2h4qb8w | Jasmine Amirzadegan, Tunc Kayikcioglu, hugh.rand , Ruth Timme, Maria Balkey | TITLE: Wastewater QC workflow in GalaxyTrakr (SSQuAWK)
AUTHORS: Jasmine Amirzadegan, Tunc Kayikcioglu, hugh.rand , Ruth Timme, Maria Balkey
[DESCRIPTION]
PURPOSE:
Step-by-step instructions for checking sequence quality for SARS-CoV-2 wastewater samples using SSQuAWK: SARS - CoV - 2 Sequence Quality Assuranc... | ["[Account set up] Create a GalaxyTrakr account here: https://account.galaxytrakr.org/Account/Register", "[Account set up] Log into your GalaxyTrakr account: https://galaxytrakr.org", "[Create a new history] Create a new history. \n\nWe recommend creating a new history for each new MiSeq Run and including the flow-cell... |
33,448 | SOP Appendix for Thymus | null | dx.doi.org/10.17504/protocols.io.bcwgixbw | null | Franchesca Farris | TITLE: SOP Appendix for Thymus
AUTHORS: Franchesca Farris
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is the appendix for the Thymus SOP. </div></div>
[STEPS]
?. | [] |
62,981 | Rachael Ray CBD Gummies - Rachael Ray CBD Gummies , Quit Smoking and 100% Pain Relief! | 3 | dx.doi.org/10.17504/protocols.io.81wgb6qrylpk/v1 | https://www.protocols.io/view/rachael-ray-cbd-gummies-rachael-ray-cbd-gummies-qu-b9rdr526 | RachaelRaycost | TITLE: Rachael Ray CBD Gummies - Rachael Ray CBD Gummies , Quit Smoking and 100% Pain Relief!
AUTHORS: RachaelRaycost
[DESCRIPTION]
The extraction of this is finished by dependable sources that continually utilize moderate extraction measures. Your body benefits from CBD. It urges you to quiet down and get the recup... | [] |
103,659 | NEB #T4010 Monarch Mag Viral DNA/ RNA Extraction Kit Protocol for KingFisher Flex Automated Isolation of Viral DNA/RNA from Wastewater Samples Following Ceres Nanotrap Enrichment | 0 | dx.doi.org/10.17504/protocols.io.n92ld87o9v5b/v1 | https://www.protocols.io/view/neb-t4010-monarch-mag-viral-dna-rna-extraction-kit-dhgj33un | Juliet Bonnevie | TITLE: NEB #T4010 Monarch Mag Viral DNA/ RNA Extraction Kit Protocol for KingFisher Flex Automated Isolation of Viral DNA/RNA from Wastewater Samples Following Ceres Nanotrap Enrichment
AUTHORS: Juliet Bonnevie
[DESCRIPTION]
The Monarch Mag Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based... | ["[Part I. Prepare the KingFisher Flex Instrument] Ensure the instrument is equipped with the KingFisher Flex 96 Deep Well head and the KingFisher Flex 96 heating block.\nIMPORTANT: The heat block must be compatible with the KingFisher 96 microplate (200 μl).", "[Part I. Prepare the KingFisher Flex Instrument] Ensure t... |
48,177 | MojoSort™ Human CD56 Nanobeads Column Protocol | 4 | null | https://www.protocols.io/view/mojosort-human-cd56-nanobeads-column-protocol-btarnid6 | Ken Lau | TITLE: MojoSort™ Human CD56 Nanobeads Column Protocol
AUTHORS: Ken Lau
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">MojoSort™ Human CD56 Nanobeads Column Protocol</span><span></span></div></div>
[STEPS]
?. [Protocol]
Prepare cells from your tissue of interest or... | ["[Protocol]\nPrepare cells from your tissue of interest or blood without lysing erythrocytes.", "[Protocol]\nIn the final wash of your sample preparation, resuspend the cells in MojoSort™ Buffer by adding up to 4 mL in a 5 mL (12 x 75 mm) polypropylene tube.Note: Keep MojoSort™ Buffer on ice throughout the procedure."... |
34,290 | Supplemental Methods for: Sonographic B-lines, Fluid Resuscitation, and Hypoxemia in Malawian Patients with Suspected Sepsis | null | dx.doi.org/10.17504/protocols.io.bdqsi5we | null | Richard J Wang, Grace Katha, Miriam Phiri, Philip Delbridge, Stephen B Gordon, Carolyn S Calfee, Laurence Huang, Jamie Rylance | TITLE: Supplemental Methods for: Sonographic B-lines, Fluid Resuscitation, and Hypoxemia in Malawian Patients with Suspected Sepsis
AUTHORS: Richard J Wang, Grace Katha, Miriam Phiri, Philip Delbridge, Stephen B Gordon, Carolyn S Calfee, Laurence Huang, Jamie Rylance
[STEPS] | [] |
41,872 | FloodLAMP Inactivation Solution Prep v1.0 | 1 | dx.doi.org/10.17504/protocols.io.bk5qky5w | https://www.protocols.io/view/floodlamp-inactivation-solution-prep-v1-0-bk5qky5w | Randy True | TITLE: FloodLAMP Inactivation Solution Prep v1.0
AUTHORS: Randy True
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The Inactivation Solution includes TCEP, EDTA, NaOH, and water. It should be made in a chemical fume hood, as the TCEP is caustic. It is very straightforward to make if starting with ... | ["[Preparation]\nAdd all TCEP to tube, break glass vial tops off, using the 1000uL pipette extract the liquid with the vial inverted", "[Preparation]\nGet amounts of TCEP, EDTA, NaOH and Ultrapure at volumes shown in chart below:", "[Preparation]\nAdd EDTA", "[Preparation]\nVortex for 10s, be sure the tube is fully clo... |
94,314 | SOP for TriZOL RNA and Protein Extraction | 4 | dx.doi.org/10.17504/protocols.io.e6nvwdyn7lmk/v1 | https://www.protocols.io/view/sop-for-trizol-rna-and-protein-extraction-c8cizsue | Malu G Tansey | TITLE: SOP for TriZOL RNA and Protein Extraction
AUTHORS: Malu G Tansey
[DESCRIPTION]
SOP for TriZOL RNA and Protein Extraction
[STEPS]
SECTION: Lyse Tissue
2. In hood, add (500uL or 1000uL depending on tissue size) ice cold TRIzol to 2mL tube containing sample and 1 tissue lyser bead.
SECTION: Lyse Tissue
3. Place i... | ["[Lyse Tissue] In hood, add (500uL or 1000uL depending on tissue size) ice cold TRIzol to 2mL tube containing sample and 1 tissue lyser bead.", "[Lyse Tissue] Place in Tissue lyser adaptors. Tubes in on “big side” of adaptors, “big side” facing out when lysing. Operate tissue lyser for 2min at 20Hz. Rearrange racks. R... |
null | null | null | dx.doi.org/10.17504/protocols.io.saseaee | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This document aims to standardize the protocol for processing of the pleural fluid and its application to QuantiFERON-TB Gold In-Tube (QFT-GIT; Cellestis Limited, Australia) system.</p>
[GUIDELINES]
<p><u>Goal</u>:</p>
<p>This document aims to standardize the protocol for pr... | [] |
62,169 | Getting started with Micro-Meta App Tutorial | 5 | dx.doi.org/10.17504/protocols.io.36wgq7ddxvk5/v6 | https://www.protocols.io/view/getting-started-with-micro-meta-app-tutorial-b8xzrxp6 | Alessandro Rigano, Mathias Hammer, Joel Ryan, Claire M. Brown, David Grunwald, Caterina Strambio De Castillia | TITLE: Getting started with Micro-Meta App Tutorial
AUTHORS: Alessandro Rigano, Mathias Hammer, Joel Ryan, Claire M. Brown, David Grunwald, Caterina Strambio De Castillia
[DESCRIPTION]
For quality, interpretation, reproducibility, and sharing value, microscopy images should be accompanied by detailed descriptions of... | ["[Before the tutorial] Video introduction", "[Tutorial - 1 - Download and Install Micro-Meta App] Download Micro-Meta App\n\nFollow the instructions in this step and in Video 3 of the tutorial series to download and install the stand-alone version of the Micro-Meta App.", "[Tutorial - 1 - Download and Install Micro-Me... |
23,555 | Canopy Trees Survey Protocol - Forests of Southern Québec | null | dx.doi.org/10.17504/protocols.io.29bgh2n | null | Mark Vellend, Sabine St-Jean, Anna Crofts | TITLE: Canopy Trees Survey Protocol - Forests of Southern Québec
AUTHORS: Mark Vellend, Sabine St-Jean, Anna Crofts
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Here, we describe the standardized protocol used by the </span><a href="http://www.caboscience.org/" style = "text-decoration:unde... | ["[Fieldwork Preparation]\nUndergo site reconnaissance. Select plot locations as follows:To fulfill the needs of locality-specific research questions, plots should span the gradients of interest (e.g., elevation and logging history), with relatively even coverage at different positions along the gradients. The aim is t... |
null | null | null | dx.doi.org/10.17504/protocols.io.e8mbhu6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This is a protocol for transfection of H4 (Human Brain Neuroglioma) in either 10% or 100% FBS using FuGENE HD. </p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
104,387 | Generating Nuclei Segmentations for vEM datasets using Cellpose | 0 | dx.doi.org/10.17504/protocols.io.n2bvjnq8pgk5/v1 | https://www.protocols.io/view/generating-nuclei-segmentations-for-vem-datasets-u-dh7b39in | Emma Avetissian, Alyson Petruncio, Jeff Rhoades, Aubrey Weigel, CellMap Project Team | TITLE: Generating Nuclei Segmentations for vEM datasets using Cellpose
AUTHORS: Emma Avetissian, Alyson Petruncio, Jeff Rhoades, Aubrey Weigel, CellMap Project Team
[DESCRIPTION]
This protocol details the process of generating nucleus segmentations in FIB-SEM (Focused Ion Beam Scanning Electron Microscopy) image stac... | ["[Introduction:] The purpose of this protocol is to provide comprehensive instructions on how to create nuclei segmentations in FIB-SEM datasets on OpenOrganelle.org using Cellpose. \n\nCellpose is a generalist algorithm developed by Stringer et al., (2020) to segment cellular structures. Cellpose also provides softwa... |
null | null | null | dx.doi.org/10.17504/protocols.io.k7nczme | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>In recent years, fruit flies (Drosophilidae) in cherry trees in northern China have become an increasingly serious problem. In this study, we examined the species composition and dynamics of fruit flies in cherry orchards by lure traps placed at different heights, and in thr... | [] |
93,471 | Protein PEGylation Protocol - Full Martini Coarse Grained v2.0 Protocol (MARTINI, INSANE, Gromacs simulations) | 5 | dx.doi.org/10.17504/protocols.io.eq2lyjbjqlx9/v1 | https://www.protocols.io/view/protein-pegylation-protocol-full-martini-coarse-gr-c7h7zj9n | compt.biology.agutierrez, Carmen Ili Gangas | TITLE: Protein PEGylation Protocol - Full Martini Coarse Grained v2.0 Protocol (MARTINI, INSANE, Gromacs simulations)
AUTHORS: compt.biology.agutierrez, Carmen Ili Gangas
[DESCRIPTION]
The construction of coarse-grained systems from atomistic models is a key strategy in simulating molecular-level biological systems. T... | ["[INTRODUCTION] The field of improving proteins via PEGylation remains a vibrant area of research, yet a profound understanding of the interactions between polymers and proteins is still in its infancy. To shed light on these interactions and enable predictive insights, Molecular Dynamics (MD) simulations offer a powe... |
19,564 | Supplemental II - Assay Buffer for Hexokinase Activity and step-by-step guide for radiochemical filter paper assay for HK Activity Assay | null | dx.doi.org/10.17504/protocols.io.xckfiuw | null | Janne R. Hingst, Rie D. Bjerre, Jørgen F. P. Wojtaszewski | TITLE: Supplemental II - Assay Buffer for Hexokinase Activity and step-by-step guide for radiochemical filter paper assay for HK Activity Assay
AUTHORS: Janne R. Hingst, Rie D. Bjerre, Jørgen F. P. Wojtaszewski
[STEPS] | [] |
70,152 | Prospecting for zoonotic pathogens using targeted DNA enrichment | 1 | dx.doi.org/10.17504/protocols.io.5jyl8jnzrg2w/v1 | https://www.protocols.io/view/prospecting-for-zoonotic-pathogens-using-targeted-cgrgtv3w | Egie Enabulele | TITLE: Prospecting for zoonotic pathogens using targeted DNA enrichment
AUTHORS: Egie Enabulele
[DESCRIPTION]
There are over 60 zoonoses linked to small mammals, including some of the most devastating pathogens in human history. Meanwhile, millions of museum-archived tissues are available to understand natural history... | ["[DNA extraction protocol (slight modification from DNeasy® Blood and Tissue Kit protocol for tissues)] Put ~ 10 mg – 20 mg of tissue in 1.5 mL microcentrifuge tubes (Note: for lysed tissue, put 180 µL of lysate in 1.5 mL microcentrifuge tubes and proceed to step iii)", "[DNA extraction protocol (slight modification f... |
18,531 | Lysis Aspergillus niger, extracting and purifying DNA | null | dx.doi.org/10.17504/protocols.io.wcbfasn | null | Laure Leynaud-Kieffer | TITLE: Lysis Aspergillus niger, extracting and purifying DNA
AUTHORS: Laure Leynaud-Kieffer
[STEPS]
?. [Grow the Biomass]
-4 days before the lysis, inoculate on MMA (Minimal Media Agar - see Transformation protocol for MMA recipe) at 30oC the strain that you want to analyze.
?. [Harvest the spores]
-Once enough bioma... | ["[Grow the Biomass]\n-4 days before the lysis, inoculate on MMA (Minimal Media Agar - see Transformation protocol for MMA recipe) at 30oC the strain that you want to analyze.", "[Harvest the spores]\n-Once enough biomass grew on the slants, you are ready to do the lysis-Make sure that before you use the entire slant ... |
80,974 | Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs) | 1 | dx.doi.org/10.17504/protocols.io.rm7vzbp14vx1/v1 | https://www.protocols.io/view/localised-axotomy-of-human-cortical-neurons-cns-fr-ctbnwime | Quyen Do, Federico Nebuloni, Richard Wade-Martins | TITLE: Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs)
AUTHORS: Quyen Do, Federico Nebuloni, Richard Wade-Martins
[DESCRIPTION]
This protocol describes the process followed to perform localised axotomy of iPSC-derived human cortical axons cultured within fluid-walled dumbb... | ["[Localised axotomy assay] On Day 20, remove the FC40 overlay from the Petri dish where human Cortical Neurons (CNs) were cultured inside fluid-walled dumbbells.", "[Localised axotomy assay] Gently add ~2 ml of coating-prep medium (see Materials) to destroy every fluid wall without peeling cells off the substrate.", "... |
71,479 | MAP-2 & GFAP Staining Protocol | 4 | dx.doi.org/10.17504/protocols.io.q26g7y4b1gwz/v1 | https://www.protocols.io/view/map-2-amp-gfap-staining-protocol-ch2xt8fn | Machlusil Husna, Kusworini Handono, Hidayat Sujuti, Aulanni'am, Ettie Rukmigarsari | TITLE: MAP-2 & GFAP Staining Protocol
AUTHORS: Machlusil Husna, Kusworini Handono, Hidayat Sujuti, Aulanni'am, Ettie Rukmigarsari
[DESCRIPTION]
Neurodegeneration due to neurotoxicity is one of the phenomena in temporal lobe epilepsy. Experimentally, hippocampal excitotoxicity process can occur due to kainic acid e... | ["[Sample Preparation] -Tissue slices were fixed using 4% PFA for 15 minutes\n-Wash with PBS for 3 times (5 minutes each)\n-Remove PBS and add 0,1% Triton X-100 for 30 minutes\n-Wash with PBS for 3 times (5 minutes each)\n-Add 10% BSA for 30 minutes at room temperature", "[Assay Procedure] -Incubate with primary antibo... |
11,564 | Expression and Purification of TailSpike 1 (TSP1) Protein from E. coli | 1 | dx.doi.org/10.17504/protocols.io.pikdkcw | https://www.protocols.io/view/expression-and-purification-of-tailspike-1-tsp1-pr-pikdkcw | Harley King | TITLE: Expression and Purification of TailSpike 1 (TSP1) Protein from E. coli
AUTHORS: Harley King
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocoll adds greater clarity and stepwise-discriptions regarding the purification of tailspike protein 1 (TSP1). The structure of TSP1 is 4OJ5. </... | ["[Prepare LB Broth and Grow Overnight Culture]\nPrepare 5-6L of LB broth in 4 liter baffled flasks. Sterilize for 20min. Cool in 37C with shaking From a plate, inoculate a single colony into 300mL LB broth with 200ul carbenicillin (50mg/ml). Grow Overnight.\nCooling LB broth in shaker at 37C with minimal shaking reduc... |
null | null | null | dx.doi.org/10.17504/protocols.io.e6bbhan | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p><strong>Protocol of dinoflagellate cell transformation</strong></p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
39,535 | Homogenate of A. cervicornis | 4 | null | https://www.protocols.io/view/homogenate-of-a-cervicornis-biupkevn | Stephanie Rosales | TITLE: Homogenate of A. cervicornis
AUTHORS: Stephanie Rosales
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is to study disease in </span><span style = "font-style:italic;">Acropora cervicornis</span><span> by using a disease homogenate method. These methods are adapted from m... | ["Collect fragments of A. cervicornis showing signs of active white-band disease. These fragments are preferably collected from the same area and with similar disease progression.", "Hold diseased fragment over Ziploc bag (** this will require two people**) and remove tissue by air brushing.Per fragment, remove ~10 cm2... |
41,823 | RNAPath Target Identification via Fluorescent Hybridization | 4 | dx.doi.org/10.17504/protocols.io.bk37kyrn | https://www.protocols.io/view/rnapath-target-identification-via-fluorescent-hybr-bk37kyrn | Devin Willis | TITLE: RNAPath Target Identification via Fluorescent Hybridization
AUTHORS: Devin Willis
[DESCRIPTION]
<div class = "text-blocks"></div>
[STEPS]
?. [Sample Preperation]
Suspend RNA pellet in
[TE Buffer]
?. [Hybridization]
Add to the RNA suspension
[Molecular Instruments COVID Probe Set]
?. [Hybridization]
Place Mole... | ["[Sample Preperation]\nSuspend RNA pellet in\n[TE Buffer]", "[Hybridization]\nAdd to the RNA suspension\n[Molecular Instruments COVID Probe Set]", "[Hybridization]\nPlace Molecular Instruments Alexa Flour 488 B1 HCR Amplifier in for\n90 °C", "[Hybridization]\nRemove Molecular Instruments Alexa Flour 488 B1 HCR Ampli... |
108,810 | S. palustris leaves sampling and RNA extraction | 0 | dx.doi.org/10.17504/protocols.io.6qpvr85jzlmk/v1 | https://www.protocols.io/view/s-palustris-leaves-sampling-and-rna-extraction-dnhi5b4e | Maria Stefanie Dwiyanti | TITLE: S. palustris leaves sampling and RNA extraction
AUTHORS: Maria Stefanie Dwiyanti
[DESCRIPTION]
This is a protocol for leaf sampling of S. palustris in natural habitats as well as RNA extraction method using CTAB+PVP buffer.
The extraction will require 3 days.
[BEFORE_START]
1. Confirm the SDS (Safety Data She... | ["[Collecting S. palustris from field] Prepare RNAlater solution in 50 ml falcon tubes.", "[Collecting S. palustris from field] Choose young leaves without fiber. \n \nCut leaves into small pieces into the RNAlater solution. Proportion of leaves and RNAlater solution should be maximum 1:5.", "[Collecting S. palustris f... |
91,119 | B-5 BLOOD SHIPPING | 4 | dx.doi.org/10.17504/protocols.io.4r3l22bopl1y/v1 | https://www.protocols.io/view/b-5-blood-shipping-c48pyzvn | REDI-NET Consortium | TITLE: B-5 BLOOD SHIPPING
AUTHORS: REDI-NET Consortium
[DESCRIPTION]
This protocol describes blood shipping.
[GUIDELINES]
OBJECTIVE
To outline steps for proper packaging and shipping of preserved blood samples from a REDI-NET Silver Lab to a REDI-NET Gold Lab.
SUMMARY/SCOPE
The overarching aim of the REDI-NET is t... | ["[SAMPLE PREPARATION] Primary holding sample holding containers must be leak-proof. Samples should be preserved in appropriate preservative, if applicable (REDI-NET Blood Processing SOP B-2). Ensure that the lids are tightly closed to prevent leaking of storage media while in transi.", "[SAMPLE PREPARATION] Pack vials... |
39,177 | Nickel-NTA Protein Purification | 4 | dx.doi.org/10.17504/protocols.io.bihhkb36 | https://www.protocols.io/view/nickel-nta-protein-purification-bihhkb36 | Nay Chi Khin, Diep Ganguly, Suyan Yee, Kai Xun Chan | TITLE: Nickel-NTA Protein Purification
AUTHORS: Nay Chi Khin, Diep Ganguly, Suyan Yee, Kai Xun Chan
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Protocol to purify 6xHis-tagged recombinant proteins expressed in </span><span style = "font-style:italic;">E. coli</span><span> using Ni-NTA pull... | ["[Prepare buffers]\nBuffer recipesResuspension buffer50 mM Tris-HCl, pH 8.02 mM EDTA1X Binding buffer 50 mM sodium phosphate buffer, pH 8.0 (ensure >1 pH units away from pI of expressed protein)500 mM sodium chloride0.5% Triton X-10010% glycerol10 mM imidazoleN.b. This should reflect the cell lysis buffer (e.g. https:... |
null | null | null | dx.doi.org/10.17504/protocols.io.ng8dbzw | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol is a modification of a previously published method by Wilgenburg et al (<a href="https://www.ncbi.nlm.nih.gov/pubmed/23951090" target="_blank">PLoS One, 2013</a>) to obtain monocytes and Dendritic cells (DCs) from induced Pluripotent Stem Cells (iPSCs) lines. </... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.gvtbw6n | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol describes the library preparation for Illumina sequencing. It accompanies the <em>GigaScience</em> publication:</p>
<p> </p>
<p>Benjamin Istace, et al. (2017) De novo assembly and population genomic survey of natural yeast isolates with the Oxford Nanopore MinIO... | [] |
null | null | null | dx.doi.org/10.17504/protocols.io.hvdb626 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Luminex Milliplex Cytokine/Chemokine 22-plex MAG manufacturer's protocol.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
105,883 | Immunofluorescence protocol for FFPE human post-mortem brain sections to detect alpha-synuclein and tau pathology | 0 | dx.doi.org/10.17504/protocols.io.n92ld8nxxv5b/v1 | https://www.protocols.io/view/immunofluorescence-protocol-for-ffpe-human-post-mo-djm34k8n | Felicia Suteja, Hongyun Li, YuHong Fu | TITLE: Immunofluorescence protocol for FFPE human post-mortem brain sections to detect alpha-synuclein and tau pathology
AUTHORS: Felicia Suteja, Hongyun Li, YuHong Fu
[DESCRIPTION]
This protocol details the method for preparing and staining human formalin-fixed, paraffin embedded post mortem brain tissue to detect al... | ["[Day I - Tissue Prep] Place slides with FFPE human brain tissues on a slide rack and bake in a 60 °C oven for 60 min", "[De-paraffinize] 2. Continue using the slide rack, submerge slides in the following solution to de-paraffinize: \n a. HistoChoice: 2 x 7 min\n b. 100% ethanol: 2 x 3 min \n c. 95% eth... |
null | null | null | dx.doi.org/10.17504/protocols.io.mcec2te | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>This protocol provides an efficient and standardized way to prepare samples ready for mass spec analysis.</p>
[BEFORE_START]
<p>Prepare solutions as described in the steps.</p>
[STEPS]
?.
?.
?.
?.
?.
?.
?.
?.
?.
?.
?. | [] |
21,848 | n-PCR for a single sample | null | dx.doi.org/10.17504/protocols.io.zjyf4pw | null | Matias Ruggieri, Carolina Berini, Nicolas Ducasa, Miroslav Malkovsky, Paul Fisch, Mirna Biglione | TITLE: n-PCR for a single sample
AUTHORS: Matias Ruggieri, Carolina Berini, Nicolas Ducasa, Miroslav Malkovsky, Paul Fisch, Mirna Biglione
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">HTLV-1 diagnosis using PCR is based on the amplification of the viral DNA sequences. This is usually conducted us... | ["Equipment and MaterialsPipettes10µl presterilized filter tips20µl presterilized filter tips200µl presterilized filter tips1000µl presterilized filter tipsThermal Cycle (we used TProfessional TRIO)Electrophoresis equipment", "Reagents and ChemicalsTaq DNA Polymerase kit (1000 U), by Qiagen, Catalog #: Cat No./ID: 2012... |
81,270 | 3. Taxon Group: Bryozoa | 4 | dx.doi.org/10.17504/protocols.io.x54v9d6mpg3e/v1 | https://www.protocols.io/view/3-taxon-group-bryozoa-ctkwwkxe | John Bishop, Inez Januszczak | TITLE: 3. Taxon Group: Bryozoa
AUTHORS: John Bishop, Inez Januszczak
[DESCRIPTION]
This is part of the collection "DToL Taxon-specific Standard Operating Procedures (SOPs) for Marine Metazoa", lead by the Other Metazoa Working Group. The SOP collection contains guidance on how to process the various marine Metazoa spe... | [] |
77,070 | Synchronization of kinetic and kinematic hand tasks during awake craniotomy | 5 | dx.doi.org/10.17504/protocols.io.5jyl89638v2w/v1 | https://www.protocols.io/view/synchronization-of-kinetic-and-kinematic-hand-task-cphnvj5e | ltaquet, bjconway, Timothy F Boerger, Sarah C Young, stschwartz, Brian Schmit, maxkrucoff | TITLE: Synchronization of kinetic and kinematic hand tasks during awake craniotomy
AUTHORS: ltaquet, bjconway, Timothy F Boerger, Sarah C Young, stschwartz, Brian Schmit, maxkrucoff
[DESCRIPTION]
Title: Synchronization of kinetic and kinematic hand tasks with electrocorticography and cortical stimulation during awake ... | ["[Constructing Synchronization Circuit] Obtaining Circuit Parts and Devices\n\nThe TekScan and CyberGlove system devices can be procured directly from the companies. Any clinical EEG device used for seizure monitoring during an awake craniotomy and cortical mapping can be used if it has at least two parallel DC channe... |
71,032 | Fast scan cyclic voltammetry | 4 | dx.doi.org/10.17504/protocols.io.kxygx9py4g8j/v1 | https://www.protocols.io/view/fast-scan-cyclic-voltammetry-chkyt4xw | Harry Xenias, Savio Chan, Loukia Parisiadou | TITLE: Fast scan cyclic voltammetry
AUTHORS: Harry Xenias, Savio Chan, Loukia Parisiadou
[DESCRIPTION]
This protocol describes the ex vivo fast-scan cyclic voltammetry (FSCV) technique for detecting dopamine within the dorsal striatum in mice.
[STEPS]
SECTION: Carbon fiber electrode preparation
1. Suction a single c... | ["[Carbon fiber electrode preparation] Suction a single carbon fiber (7μm diameter, Goodfellow) into a capillary tube (Sutter).", "[Carbon fiber electrode preparation] Load the carbon fiber/capillary tube into a puller (Narishinge), and under the microscope, hand-cut (scalpel blade) fiber tips 30-100μm past the capilla... |
29,295 | Chondrocyte isolation from human cartilage | null | dx.doi.org/10.17504/protocols.io.8uphwvn | null | Sarah Snelling | TITLE: Chondrocyte isolation from human cartilage
AUTHORS: Sarah Snelling
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A standard methodology used within our group for extracting chondrocytes from human cartilage obtained as waste tissue at the time of joint replacement. Chondrocytes obtained thr... | ["[Collagenase preparation]\nMake up Collagenase solution (1mg/ml) in standard medium, allow to dissolve and sterile filter through a 0.45um followed by 0.2um syringe filter. The 0.45um filter step is essential to enable efficient filtration. Collagenase mixture can be used fresh or immediately frozen and used up to 6 ... |
20,780 | UC Davis - Erectile Dysfunction | null | dx.doi.org/10.17504/protocols.io.yikfucw | null | Li-Ming Jin | TITLE: UC Davis - Erectile Dysfunction
AUTHORS: Li-Ming Jin
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Erectile function is well regulated by vasorelaxant and vasoconstrictive mechanisms, which can be perturbed by... | ["Rats or mice are anesthetized with an intraperitoneal injection with 50 mg pentobarbital/kg weight.", "A midline incision was made from umbilicus to pubis.", "The penis was denuded of skin, and the prepuce circumcised. The testes and epididymides were repositioned into the abdomen after they were divided from their s... |
104,749 | Manual Aseptic Sort Procedure for BD FACS Aria II/III/Fusion | 0 | null | https://www.protocols.io/view/manual-aseptic-sort-procedure-for-bd-facs-aria-ii-diim4cc6 | Jamie C Tijerina | TITLE: Manual Aseptic Sort Procedure for BD FACS Aria II/III/Fusion
AUTHORS: Jamie C Tijerina
[DESCRIPTION]
This is an alternative to the automated aseptic sort procedure designed to decontaminate the lines of the BD FACS Aria Fusion instrument. Bypasses filters and other internal components that may be damaged by 10%... | ["Perform decontamination of tank (see SOP). The preference is to use a spare 10L main tank when performing this procedure.", "Fill the tank with 4L of 10% bleach.", "Connect the air line and the sheath line directly to the tank. Do not connect the filter as it cannot withstand bleach.", "Place an already damaged nozzl... |
null | null | null | dx.doi.org/10.17504/protocols.io.mqcc5sw | null | null | TITLE: No Title
AUTHORS:
[STEPS]
?.
?.
?. | [] |
84,914 | Canine Enteric Virus Detection Assays | 1 | dx.doi.org/10.17504/protocols.io.e6nvwd5z9lmk/v1 | https://www.protocols.io/view/canine-enteric-virus-detection-assays-cw6sxhee | Come J Thieulent, Mariano Carossino, Laura Peak, Udeni B. R. Balasuriya | TITLE: Canine Enteric Virus Detection Assays
AUTHORS: Come J Thieulent, Mariano Carossino, Laura Peak, Udeni B. R. Balasuriya
[DESCRIPTION]
The Canine Enteric Virus Detection Assays is intended as an in vitro veterinary reagent set, based on Reverse Transcription quantitative PCR (RT-qPCR), for the detection of canine... | ["[Reaction Set Up] Thaw all reagents on ice.", "[Reaction Set Up] Centrifuge all reagents on a benchtop centrifuge to ensure no liquid is in cap and keep on ice", "[Reaction Set Up] Setup the Master Mix according to the following table 1:", "[PROGRAMMING THE THERMOCYCLER] Select the following fluorescence channels: AB... |
21,768 | ALM Window Surgery | 1 | dx.doi.org/10.17504/protocols.io.zhgf33w | https://www.protocols.io/view/alm-window-surgery-zhgf33w | JJ Kim | TITLE: ALM Window Surgery
AUTHORS: JJ Kim
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Head Post and Cranial Window Outline</div><div class = "text-block">Janelia Research Campus</div><div class = "text-block">Svoboda Lab</div><div class = "text-block">Bryan Maclennan (December 2013)</div><div cl... | ["[Animal Preparation]\nWeigh the mouse and transfer it to a new cage if needed (i.e. if it's group housed, or if its cage is dirty.)If mice do not have ear tags - denote first mouse removed as the first animal number etc.and cross out number on the original cage.If the mice have ear tags, use the animal number that co... |
82,373 | Bone Marrow Derived Macrophage (BMDM) differentiation and maintenance | 4 | dx.doi.org/10.17504/protocols.io.j8nlkw7bwl5r/v1 | https://www.protocols.io/view/bone-marrow-derived-macrophage-bmdm-differentiatio-cupdwvi6 | Narayana Yadavalli, Shawn M. Ferguson | TITLE: Bone Marrow Derived Macrophage (BMDM) differentiation and maintenance
AUTHORS: Narayana Yadavalli, Shawn M. Ferguson
[DESCRIPTION]
This protocol describes the isolation of bone marrow derived macrophages.
[STEPS]
1.
Euthanize mice via CO2 or isoflurane inhalation and cervical dislocation.
2. Collect femurs ... | ["Euthanize mice via CO2 or isoflurane inhalation and cervical dislocation.", "Collect femurs and flush cavities with 5 mL ice-cold PBS.", "Then, collect bone marrow cells by centrifugation 0.3 rcf, 5 min.", "Resuspend the resulting pellet and differentiate for 6 days in culture media containing: DMEMF12 supplemented w... |
61,524 | Panicle Ratio Network: A high-throughput dynamic phenotype recognition model based on ultra-high-definition unmanned aerial vehicle images for rice panicle analysis in fields | 1 | dx.doi.org/10.17504/protocols.io.bp2l6176rvqe/v1 | https://www.protocols.io/view/panicle-ratio-network-a-high-throughput-dynamic-ph-b8bursnw | Ziyue Guo, Chenghai Yang, Wangnen Yang, Guoxing Chen, Zhao Jiang, Botao Wang, Jian Zhang | TITLE: Panicle Ratio Network: A high-throughput dynamic phenotype recognition model based on ultra-high-definition unmanned aerial vehicle images for rice panicle analysis in fields
AUTHORS: Ziyue Guo, Chenghai Yang, Wangnen Yang, Guoxing Chen, Zhao Jiang, Botao Wang, Jian Zhang
[DESCRIPTION]
In order to increase the ... | ["High-resolution images of rice at heading stage were obtained by camera equipment.", "After image collection, some plots were selected to investigate their panicle number and tillering number.", "The plot to be observed was cropped from the original image, and the corresponding plot image was annotated according to t... |
null | null | null | dx.doi.org/10.17504/protocols.io.i3mcgk6 | null | null | TITLE: No Title
AUTHORS:
[DESCRIPTION]
<p>Long-read sequencing (e.g. Nanopore's MinION or PacBio) has the potential to dramatically improve genome assembly but the quality of the reads is critically dependent upon the quality of the input gDNA. <br /><br />Here I describe the extraction of high molecular weight DNA f... | [] |
20,084 | Making Differentiation Media for SH-SY5Y | null | dx.doi.org/10.17504/protocols.io.xuufnww | null | Kenneth Schackart | TITLE: Making Differentiation Media for SH-SY5Y
AUTHORS: Kenneth Schackart
[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This describes how to make differentiation medium for SH-SY5Y using all-</span><span style = "font-style:italic;">trans-</span><span>retinoic acid.</span></div></div>
[ST... | ["[Make ATRA Stock Solution]\nIn a 15 mL centrifuge tube, add ethanol andautoclaved deionized water. This will make a 95% ethanol solution.\n14 ml\n0 ml", "[Make ATRA Stock Solution]\nWeigh out ATRA.\n5 mg\nATRA is light sensitive, so do this step with the lights off.", "[Make ATRA Stock Solution]\nTransfer of 95% EtO... |
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